intercellular junctions

Summary

Summary: Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)

Top Publications

  1. pmc Occludin: a novel integral membrane protein localizing at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Biol 123:1777-88. 1993
  2. ncbi Endothelial cell-to-cell junctions: molecular organization and role in vascular homeostasis
    Gianfranco Bazzoni
    Istituto di Ricerche Farmacologiche Mario Negri, Via Eritrea 62, I 20157 Milan, Italy
    Physiol Rev 84:869-901. 2004
  3. ncbi Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis
    P Carmeliet
    Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
    Cell 98:147-57. 1999
  4. ncbi The control of vascular integrity by endothelial cell junctions: molecular basis and pathological implications
    Elisabetta Dejana
    IFOM, The FIRC Institute of Molecular Oncology, Milan, Italy
    Dev Cell 16:209-21. 2009
  5. ncbi The PAR-aPKC system: lessons in polarity
    Atsushi Suzuki
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Cell Sci 119:979-87. 2006
  6. pmc Intercellular junction assembly, dynamics, and homeostasis
    Kathleen J Green
    Northwestern University Feinberg School of Medicine, Department of Pathology, R H Lurie Comprehensive Cancer Center, 303 E Chicago Ave Chicago, Illinois 60611, USA
    Cold Spring Harb Perspect Biol 2:a000125. 2010
  7. ncbi Endothelial cell-cell junctions: happy together
    Elisabetta Dejana
    Department of Biomolecular and Biotechnological Sciences, School of Sciences, Milan University, Italy
    Nat Rev Mol Cell Biol 5:261-70. 2004
  8. pmc The conditional inactivation of the beta-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility
    Anna Cattelino
    FIRC Institute of Molecular Oncology, 16 20139, Milan, Italy
    J Cell Biol 162:1111-22. 2003
  9. ncbi Cellular and molecular analyses of vascular tube and lumen formation in zebrafish
    Suk Won Jin
    Department of Biochemistry and Biophysics and Cardiovascular Research Institute, Program in Developmental Biology, University of California San Francisco, 1550 Fourth Street, San Francisco, CA 94143, USA
    Development 132:5199-209. 2005
  10. ncbi The immunoglobulin-like cell adhesion molecule nectin and its associated protein afadin
    Yoshimi Takai
    Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Annu Rev Cell Dev Biol 24:309-42. 2008

Detail Information

Publications283 found, 100 shown here

  1. pmc Occludin: a novel integral membrane protein localizing at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Biol 123:1777-88. 1993
    ..These findings revealed that an integral membrane protein localizing at tight junctions is now identified, which we designated as "occludin."..
  2. ncbi Endothelial cell-to-cell junctions: molecular organization and role in vascular homeostasis
    Gianfranco Bazzoni
    Istituto di Ricerche Farmacologiche Mario Negri, Via Eritrea 62, I 20157 Milan, Italy
    Physiol Rev 84:869-901. 2004
    b>Intercellular junctions mediate adhesion and communication between adjoining endothelial and epithelial cells. In the endothelium, junctional complexes comprise tight junctions, adherens junctions, and gap junctions...
  3. ncbi Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis
    P Carmeliet
    Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
    Cell 98:147-57. 1999
    ..Thus, VE-cadherin/ beta-catenin signaling controls endothelial survival...
  4. ncbi The control of vascular integrity by endothelial cell junctions: molecular basis and pathological implications
    Elisabetta Dejana
    IFOM, The FIRC Institute of Molecular Oncology, Milan, Italy
    Dev Cell 16:209-21. 2009
    ..In this review we discuss recent findings derived from these approaches and their possible integration in a common picture...
  5. ncbi The PAR-aPKC system: lessons in polarity
    Atsushi Suzuki
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Cell Sci 119:979-87. 2006
    ....
  6. pmc Intercellular junction assembly, dynamics, and homeostasis
    Kathleen J Green
    Northwestern University Feinberg School of Medicine, Department of Pathology, R H Lurie Comprehensive Cancer Center, 303 E Chicago Ave Chicago, Illinois 60611, USA
    Cold Spring Harb Perspect Biol 2:a000125. 2010
    ..We will explore signaling and trafficking machineries that drive assembly and remodeling and how these mechanisms are co-opted in human disease...
  7. ncbi Endothelial cell-cell junctions: happy together
    Elisabetta Dejana
    Department of Biomolecular and Biotechnological Sciences, School of Sciences, Milan University, Italy
    Nat Rev Mol Cell Biol 5:261-70. 2004
  8. pmc The conditional inactivation of the beta-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility
    Anna Cattelino
    FIRC Institute of Molecular Oncology, 16 20139, Milan, Italy
    J Cell Biol 162:1111-22. 2003
    ..Cultured beta-catenin -/- endothelial cells showed a different organization of intercellular junctions with a decrease in alpha-catenin in favor of desmoplakin and marked changes in actin cytoskeleton...
  9. ncbi Cellular and molecular analyses of vascular tube and lumen formation in zebrafish
    Suk Won Jin
    Department of Biochemistry and Biophysics and Cardiovascular Research Institute, Program in Developmental Biology, University of California San Francisco, 1550 Fourth Street, San Francisco, CA 94143, USA
    Development 132:5199-209. 2005
    ..These studies provide the tools and a cellular framework for the investigation of mutations affecting vasculogenesis in zebrafish...
  10. ncbi The immunoglobulin-like cell adhesion molecule nectin and its associated protein afadin
    Yoshimi Takai
    Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Annu Rev Cell Dev Biol 24:309-42. 2008
    ..Additionally, nectins regulate many other cellular activities (such as movement, proliferation, survival, differentiation, polarization, and the entry of viruses) in cooperation with other CAMs and cell surface membrane receptors...
  11. ncbi Molecular anatomy of intercellular junctions in brain endothelial and epithelial barriers: electron microscopist's view
    Andrzej W Vorbrodt
    Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, NY 10314, USA
    Brain Res Brain Res Rev 42:221-42. 2003
    ..we have tried to summarize the current knowledge on the distribution of important molecular components of intercellular junctions-both tight junctions (TJs) and adherens junctions (AJs)-at the level of ultrastructure...
  12. ncbi Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocr Rev 25:747-806. 2004
    ..Other factors such as proteases, protease inhibitors, GTPases, kinases, and phosphatases also come into play. In this review, we focus on this cellular phenomenon, recapping recent developments in the field...
  13. ncbi Angiopoietin-1 is an antipermeability and anti-inflammatory agent in vitro and targets cell junctions
    J R Gamble
    Vascular Biology Laboratory, Division of Immunology, Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science and the University of Adelaide, South Australia
    Circ Res 87:603-7. 2000
    ..Furthermore, angiopoietin-1 inhibits TNF-alpha-stimulated leukocyte transmigration. Angiopoietin-1 may thus have a major role in maintaining the integrity of endothelial monolayers...
  14. pmc Cdc42 GEF Tuba regulates the junctional configuration of simple epithelial cells
    Tetsuhisa Otani
    Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Kyoto 606 8501, Japan
    J Cell Biol 175:135-46. 2006
    ..These results suggest that Tuba controls the shaping of cell junctions through the local activation of Cdc42 and its effectors...
  15. ncbi Localization of apical epithelial determinants by the basolateral PDZ protein Scribble
    D Bilder
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 403:676-80. 2000
    ..Our results show that the lateral domain of epithelia, particularly the septate junction, functions in restricting apical membrane identity and correctly placing adherens junctions...
  16. pmc Growth control by committee: intercellular junctions, cell polarity, and the cytoskeleton regulate Hippo signaling
    Julian C Boggiano
    Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA
    Dev Cell 22:695-702. 2012
    ..review, we discuss recent findings that emphasize the cell cortex-specifically the actin cytoskeleton, intercellular junctions, and protein complexes that determine cell polarity-as a key site for Hippo pathway regulation...
  17. ncbi Regulation of cadherin-mediated adhesion in morphogenesis
    Barry M Gumbiner
    Department of Cell Biology, University of Virginia, School of Medicine, PO Box 800732, Charlottesville, Virginia 22908 0732, USA
    Nat Rev Mol Cell Biol 6:622-34. 2005
    ..Signal-transduction pathways impinge on the catenins (cytoplasmic cadherin-associated proteins), which transduce changes across the membrane to alter the state of the cadherin adhesive bond...
  18. ncbi Regulation of vascular endothelial barrier function by Epac, a cAMP-activated exchange factor for Rap GTPase
    Xavier Cullere
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA, USA
    Blood 105:1950-5. 2005
    ..Thus, Epac/Rap activation represents a new pathway for regulating endothelial cell barrier function...
  19. ncbi Nectin and afadin: novel organizers of intercellular junctions
    Yoshimi Takai
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Cell Sci 116:17-27. 2003
    ..adhesion system, consisting of nectin and afadin, also has roles in organization of a variety of intercellular junctions either in cooperation with, or independently of, cadherin...
  20. pmc Stabilizing the VE-cadherin-catenin complex blocks leukocyte extravasation and vascular permeability
    Dörte Schulte
    Department of Vascular Cell Biology, Max Planck Institute of Molecular Biomedicine, Munster, Germany
    EMBO J 30:4157-70. 2011
    ..Furthermore, in these tissues, plasticity of the VE-cadherin-catenin complex is central for the leukocyte diapedesis mechanism...
  21. pmc Selective uncoupling of p120(ctn) from E-cadherin disrupts strong adhesion
    M A Thoreson
    Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2175, USA
    J Cell Biol 148:189-202. 2000
    ..Our data suggest that p120 directly or indirectly regulates the E-cadherin-mediated transition to tight cell-cell adhesion, possibly blocking subsequent events necessary for reorganization of the actin cytoskeleton and compaction...
  22. ncbi Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission
    Stefanie Sowinski
    Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London, SW7 2AZ, UK
    Nat Cell Biol 10:211-9. 2008
    ..These data lead us to propose that HIV-1 can spread using nanotubular connections formed by short-term intercellular unions in which T cells specialize...
  23. pmc Vascular endothelial-cadherin stabilizes at cell-cell junctions by anchoring to circumferential actin bundles through alpha- and beta-catenins in cyclic AMP-Epac-Rap1 signal-activated endothelial cells
    Kazuomi Noda
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Suita, Osaka 565 8565, Japan
    Mol Biol Cell 21:584-96. 2010
    ....
  24. ncbi Differential function of Tie2 at cell-cell contacts and cell-substratum contacts regulated by angiopoietin-1
    Shigetomo Fukuhara
    Department of Structural Analysis, National Cardiovascular Center Research Institute, 5 7 1 Fujishirodai, Suita, Osaka 565 8565, Japan
    Nat Cell Biol 10:513-26. 2008
    ....
  25. pmc A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions
    W A Muller
    Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021
    J Exp Med 170:399-414. 1989
    ..This protein may mediate endothelial-specific functions restricted to the intercellular domain. It may also serve as a unique cell surface marker for the identification and purification of human endothelial cells...
  26. ncbi Epac1 regulates integrity of endothelial cell junctions through VE-cadherin
    Matthijs R H Kooistra
    Department of Physiological Chemistry, Centre for Biomedical Genetics, University Medical Center, Utrecht, The Netherlands
    FEBS Lett 579:4966-72. 2005
    ..From these results we conclude that in human umbilical vein endothelial cells Epac1 controls VE-cadherin-mediated cell junction formation and induces reorganization of the actin cytoskeleton...
  27. ncbi Basal-to-apical cadherin flow at cell junctions
    Yoshiko Kametani
    Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Nat Cell Biol 9:92-8. 2007
    ..We propose that cadherin flow may provide a mechanism for facilitating the sliding of the two contacting cell membranes in morphogenetically active cell sheets...
  28. pmc Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Aichi, Japan
    J Cell Biol 127:1617-26. 1994
    ..The coincidence of the sequence necessary for the ZO-1 association with that for the TJ localization suggests that the association with underlying cytoskeletons through ZO-1 is required for occludin to be localized at TJ...
  29. pmc Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction
    K Mandai
    Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, Kobe 651 22, Japan
    J Cell Biol 139:517-28. 1997
    ..These results suggest that l-afadin serves as a linker of the actin cytoskeleton to the plasma membrane at cell-to-cell AJ...
  30. pmc alpha-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells
    M Watabe-Uchida
    Department of Biophysics, Faculty of Science, Kyoto University, Kitashirakawa, Sakyo ku, Kyoto 606 8502, USA
    J Cell Biol 142:847-57. 1998
    ..These results indicate that the alphaE-catenin-vinculin interaction plays a role in the assembly of the apical junctional complex in epithelia...
  31. ncbi Directed actin polymerization is the driving force for epithelial cell-cell adhesion
    V Vasioukhin
    Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, The University of Chicago, Illinois 60637, USA
    Cell 100:209-19. 2000
    ..Taken together, a dynamic mechanism for intercellular adhesion is unveiled involving calcium-activated filopodia penetration and VASP/Mena-dependent actin reorganization/polymerization...
  32. ncbi A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene
    R G Fehon
    Department of Zoology, Duke University, Durham, North Carolina 27708 0325
    Development 120:545-57. 1994
    ..These data indicate that D4.1 is associated with the septate junction, and suggest that it may play a role in cell-cell interactions that are essential for normal development...
  33. pmc A unique role for nonmuscle myosin heavy chain IIA in regulation of epithelial apical junctions
    Andrei I Ivanov
    Epithelial Pathobiology Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, United States of America
    PLoS ONE 2:e658. 2007
    ..These findings provide the first direct non-pharmacological evidence of myosin II-dependent regulation of AJC dynamics in mammalian epithelia and highlight a unique role of NMMIIA in junctional biogenesis...
  34. ncbi A monoclonal antibody to vascular endothelial-cadherin inhibits tumor angiogenesis without side effects on endothelial permeability
    Monica Corada
    FIRC Institute of Molecular Oncology, Milan, Italy
    Blood 100:905-11. 2002
    ..In contrast, in growing vessels, where endothelial cells are migrating and junctions are weaker, antibody binding to EC4 may be sufficient to disrupt cell-to-cell adhesion and inhibit assembly of new vascular structures...
  35. ncbi Interaction of nectin with afadin is necessary for its clustering at cell-cell contact sites but not for its cis dimerization or trans interaction
    M Miyahara
    Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, 2 2 10 Murotani, Nishi ku, Kobe 651 2241, Japan
    J Biol Chem 275:613-8. 2000
    ..On the basis of these observations, we propose a model for the mechanism of cell-cell adhesion of nectin and roles of afadin in this mechanism...
  36. ncbi Plakoglobin: a protein common to different kinds of intercellular adhering junctions
    P Cowin
    Cell 46:1063-73. 1986
    ..also occurs at the plaques of other types of adhering junctions, including the vinculin-actin-associated intercellular junctions, e.g., the zonula adhaerens of epithelial cells and the endothelial, lens, and Sertoli cell junctions...
  37. ncbi The parting of the endothelium: miracle, or simply a junctional affair?
    C Johnson-Leger
    Department of Pathology, Centre Medical Universitaire, Switzerland
    J Cell Sci 113:921-33. 2000
    ..Passage of the leukocyte across the endothelium appears to require delocalization of certain endothelial cell molecules and proteolytic degradation of junctional complexes...
  38. pmc Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice
    Momina Mirza
    Department of Women s and Children s Health, Karolinska Institute and University Hospital, Stockholm, Sweden
    PLoS ONE 7:e37523. 2012
    ..The results show that CAR plays an essential role in development of the lymphatic vasculature in the mouse embryo by promoting appropriate formation of lymphatic endothelial cell-cell junctions...
  39. ncbi The biology of the desmosome-like junction a versatile anchoring junction and signal transducer in the seminiferous epithelium
    Pearl P Y Lie
    Population Council, Center for Biomedical Research, New York, New York, USA
    Int Rev Cell Mol Biol 286:223-69. 2011
    ..We place special emphasis on the emerging role of desmosome-like junctions as signal transducers during germ cell movement across the seminiferous epithelium...
  40. pmc Plasmodium falciparum adhesion on human brain microvascular endothelial cells involves transmigration-like cup formation and induces opening of intercellular junctions
    Ronan Jambou
    Vascular Immunology Unit, Department of Pathology and Bosch Institute, Sydney Medical School, The University of Sydney, New South Wales, Australia
    PLoS Pathog 6:e1001021. 2010
    ..This is associated with the opening of the intercellular junctions. The transfer of IRBC antigens can thus transform EC into a target for the immune response and contribute ..
  41. ncbi Shroom3-mediated recruitment of Rho kinases to the apical cell junctions regulates epithelial and neuroepithelial planar remodeling
    Tamako Nishimura
    RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Development 135:1493-502. 2008
    ..These results demonstrate that the Shroom3-ROCK interaction is crucial for the regulation of epithelial and neuroepithelial cell arrangement and remodeling...
  42. ncbi Localization of proteins to the apico-lateral junctions of Drosophila epithelia
    D F Woods
    Developmental Biology Center, University of California, Irvine, USA
    Dev Genet 20:111-8. 1997
    ....
  43. ncbi Role of afadin in vascular endothelial growth factor- and sphingosine 1-phosphate-induced angiogenesis
    Hideto Tawa
    Division of Signal Transduction, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    Circ Res 106:1731-42. 2010
    ..It is not known whether afadin, an adherens junction protein that connects immunoglobulin-like adhesion molecule nectins to the actin cytoskeleton and binds activated Rap1, plays a role in angiogenesis...
  44. ncbi Breaking the VE-cadherin bonds
    Julie Gavard
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    FEBS Lett 583:1-6. 2009
    ..These findings provide evidence on how endothelial cell-cell junctions impact the vascular network, and change our perception about normal and aberrant angiogenesis...
  45. pmc N-cadherin association with lipid rafts regulates its dynamic assembly at cell-cell junctions in C2C12 myoblasts
    Marie Causeret
    Centre de Recherches de Biochimie Macromoleculaire, Centre National de la Recherche Scientifique Formation de Recherche en Evolution 2593, 34293 Montpellier, France
    Mol Biol Cell 16:2168-80. 2005
    ..Homophilic interactions and F-actin association of N-cadherin are both required for its association to lipid rafts. We thus identify lipid rafts as new regulators of cadherin-mediated cell adhesion...
  46. pmc Characterization of integrin-tetraspanin adhesion complexes: role of tetraspanins in integrin signaling
    F Berditchevski
    CRC Institute for Cancer Studies, The University of Birmingham, Edgbaston, Birmingham, United Kingdom B15 2TA
    J Cell Biol 146:477-92. 1999
    ..These results show that tetraspanins can modulate integrin signaling, and point to a mechanism by which TM4SF proteins regulate cell motility...
  47. ncbi Inhibition of invasion of epithelial cells by Tiam1-Rac signaling
    P L Hordijk
    Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, Netherlands
    Science 278:1464-6. 1997
    ..These data suggest an invasion-suppressor role for Tiam1 and Rac in epithelial cells...
  48. pmc Myosin IXa regulates epithelial differentiation and its deficiency results in hydrocephalus
    Marouan Abouhamed
    Institute of General Zoology and Genetics, Westfalian Wilhelms University, 48149 Munster, Germany
    Mol Biol Cell 20:5074-85. 2009
    ..resulted in a distorted ependyma due to irregular epithelial cell morphology and altered organization of intercellular junctions. Ependymal cells occasionally delaminated, forming multilayered structures that bridged the CSF-filled ..
  49. ncbi Loss of epithelial integrity resulting from E-cadherin dysfunction predisposes airway epithelial cells to adenoviral infection
    Y Man
    Cell Biology Department and Clinical Virology Department, Medicines Research Centre, GlaxoWellcome, Stevenage, United Kingdom
    Am J Respir Cell Mol Biol 23:610-7. 2000
    ..cadherins are important because these adhesion molecules regulate formation and maintenance of epithelial intercellular junctions. To study the importance of airway epithelial integrity in determining susceptibility to virus infection, ..
  50. pmc E-cadherin and APC compete for the interaction with beta-catenin and the cytoskeleton
    J Hülsken
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    J Cell Biol 127:2061-9. 1994
    ....
  51. ncbi Missing-in-metastasis MIM/MTSS1 promotes actin assembly at intercellular junctions and is required for integrity of kidney epithelia
    Juha Saarikangas
    Institute of Biotechnology, PO Box 56, University of Helsinki, 00014 Finland
    J Cell Sci 124:1245-55. 2011
    ..mice displayed a severe urinary concentration defect caused by compromised integrity of kidney epithelia intercellular junctions, which led to bone abnormalities and end-stage renal failure...
  52. pmc Structure and function of intercellular junctions in human cervical and vaginal mucosal epithelia
    Caitlin D Blaskewicz
    Department of Molecular Medicine, Obstetrics and Gynecology, and Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Biol Reprod 85:97-104. 2011
    ..To further define the molecular structure of the intercellular junctions, select junctional molecules were localized in the endocervical, ectocervical, and vaginal epithelium by ..
  53. pmc Differential localization of VE- and N-cadherins in human endothelial cells: VE-cadherin competes with N-cadherin for junctional localization
    P Navarro
    Laboratory of Vascular Biology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy
    J Cell Biol 140:1475-84. 1998
    ..Competition between cadherins for their clustering at intercellular junctions in the same cell has never been described before...
  54. pmc Nectin/PRR: an immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with Afadin, a PDZ domain-containing protein
    K Takahashi
    Takai Biotimer Project, ERATO, Japan Science and Technology Corp, c o JCR Pharmaceuticals Co, Ltd, Kobe 651 2241, Japan
    J Cell Biol 145:539-49. 1999
    ..We rename PRR as nectin (taken from the Latin word "necto" meaning "to connect")...
  55. ncbi The HA proteins of botulinum toxin disrupt intestinal epithelial intercellular junctions to increase toxin absorption
    Takuhiro Matsumura
    Laboratory for Infection Cell Biology, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Cell Microbiol 10:355-64. 2008
    ..These studies show that the 16S BoNT complex is a multifunctional protein assembly equipped with the machinery to efficiently breach the intestinal barrier and act systemically on peripheral nerves...
  56. ncbi Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia
    M Koppen
    Program in Cellular and Molecular Biology, University of Wisconsin Madison, 1117 West Johnson Street, Madison, Wisconsin 53706, USA
    Nat Cell Biol 3:983-91. 2001
    ..We conclude that LET-413 and DLG-1 cooperatively control AJM-1 localization and that AJM-1 controls the integrity of a distinct apical junctional domain in C. elegans...
  57. pmc Actomyosin contractility and Discs large contribute to junctional conversion in guiding cell alignment within the Drosophila embryonic epithelium
    Robert P Simone
    University of Pennsylvania Medical School, Department of Cell and Developmental Biology, 421 Curie Boulevard, Philadelphia, PA 19104 6048, USA
    Development 137:1385-94. 2010
    ..Indeed, depletion of Myosin II or dlg disrupts these processes. These results show that tight spatial regulation of actomyosin contractility is required to produce this high-energy arrangement of cells...
  58. pmc Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: function in junctional complexes and leukocyte adhesion
    Chrystelle Lamagna
    Department of Pathology and Immunology, Centre Medical Universitaire, 1204 Geneva, Switzerland
    Mol Biol Cell 16:4992-5003. 2005
    ..We demonstrate that the modulation of JAM-C localization in junctional complexes is a new regulatory mechanism for alpha(M)beta2-dependent adhesion of leukocytes...
  59. ncbi Willin and Par3 cooperatively regulate epithelial apical constriction through aPKC-mediated ROCK phosphorylation
    Takashi Ishiuchi
    RIKEN Center for Developmental Biology, Chuo Ku, Kobe 650 0047, Japan
    Nat Cell Biol 13:860-6. 2011
    ..Thus, we have uncovered a Willin/Par3-aPKC-ROCK pathway that controls epithelial apical morphology...
  60. pmc VE-PTP maintains the endothelial barrier via plakoglobin and becomes dissociated from VE-cadherin by leukocytes and by VEGF
    Astrid F Nottebaum
    Max Planck Institute of Molecular Biomedicine, Munster, Germany
    J Exp Med 205:2929-45. 2008
    ..In conclusion, leukocytes interacting with endothelial cells rapidly dissociate VE-PTP from VE-cadherin, weakening endothelial cell contacts via a mechanism that requires plakoglobin but not beta-catenin...
  61. pmc Regulated assembly of tight junctions by protein kinase C
    R O Stuart
    Department of Medicine, Brigham and Women s Hospital, Boston, MA, USA
    Proc Natl Acad Sci U S A 92:6072-6. 1995
    ..Identity of the 330- and 65-kDa phosphoproteins remains to be determined, but the 65-kDa protein may be occludin. The mass of this complex and the incorporation of ZO-1 into the Triton X-100-insoluble cytoskeleton were not PKC dependent...
  62. ncbi Organization and function of septate junctions: an evolutionary perspective
    Swati Banerjee
    Department of Cell and Molecular Physiology, Curriculum in Neurobiology, UNC Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7545, USA
    Cell Biochem Biophys 46:65-77. 2006
    In most cell types, distinct forms of intercellular junctions have been visualized at the ultrastructural level...
  63. pmc N-cadherin/p120 catenin association at cell-cell contacts occurs in cholesterol-rich membrane domains and is required for RhoA activation and myogenesis
    Nicolas Taulet
    Centre de Recherche de Biochimie Macromoleculaire, Universités Montpellier 2 et 1, CNRS, Unité Mixte de Recherche 5237, Institut Federatif de Recherche 122, 1919 Route de Mende, 34293 Montpellier, France
    J Biol Chem 284:23137-45. 2009
    ..Thus, a functional N-cadherin-catenin complex occurs in cholesterol-rich membrane microdomains which allows the recruitment of RhoA and the regulation of its activity during myogenesis induction...
  64. ncbi The slit diaphragm: a signaling platform to regulate podocyte function
    Tobias B Huber
    Pathology and Immunology, Washington University St Louis, St Louis, Missouri, USA
    Curr Opin Nephrol Hypertens 14:211-6. 2005
    ..This review focuses on the most recent evidence elucidating the dynamic functions of the slit diaphragm and stresses the importance of slit diaphragm proteins in mediating signal transduction in the podocyte...
  65. pmc Molecular characterization and tissue distribution of ZO-2, a tight junction protein homologous to ZO-1 and the Drosophila discs-large tumor suppressor protein
    L A Jesaitis
    Program in Cell and Development Biology, Harvard Medical School, Boston, Massachusetts 02115
    J Cell Biol 124:949-61. 1994
    ..Nagafuchi, S. Tsukita, and Sh. Tsukita. 1991. J. Cell Biol. 115:1449-1462). Thus it appears that ZO-2 is not a component of the fascia adherens, and that unlike ZO-1, this protein is restricted to the epithelial tight junction...
  66. ncbi Intercellular junctions and cellular polarity: the PAR-aPKC complex, a conserved core cassette playing fundamental roles in cell polarity
    S Ohno
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9, Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Curr Opin Cell Biol 13:641-8. 2001
    ..Recent studies on the role of this PAR-aPKC complex in epithelial cell polarization provide new insights into the molecular basis of epithelial junctional formation and cell polarity...
  67. ncbi Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members
    M Aurrand-Lions
    Department of Pathology, Centre Medical Universitaire, Geneva, Switzerland
    Blood 98:3699-707. 2001
    ..Furthermore, it was shown that JAM-1 and JAM-2 differentially regulate paracellular permeability, suggesting that the presence of JAM-1, -2, or -3 in vascular junctions may play a role in regulating vascular function in vivo...
  68. ncbi Cooperative regulation of cell polarity and growth by Drosophila tumor suppressors
    D Bilder
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Science 289:113-6. 2000
    ..scrib, dlg, and lgl show strong genetic interactions. Our data indicate that the three tumor suppressors act together in a common pathway to regulate cell polarity and growth control...
  69. pmc A functional assay for proteins involved in establishing an epithelial occluding barrier: identification of a uvomorulin-like polypeptide
    B Gumbiner
    J Cell Biol 102:457-68. 1986
    ..cm2. When the monolayers were incubated at 37 degrees C without Ca2+, the intercellular junctions opened and the transmonolayer resistance dropped to the value of a bare filter, i.e., less than 40 ohm ...
  70. pmc Feedback interactions between cell-cell adherens junctions and cytoskeletal dynamics in newt lung epithelial cells
    C M Waterman-Storer
    Department of Cell Biology and Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 11:2471-83. 2000
    ..These results indicate that a MT plus end capping protein is regulated by cell-cell contact, and in turn, that MT growth regulates the maintenance of adherens junctions contacts in epithelia...
  71. ncbi Breaking down the blood-brain barrier: signaling a path to cerebral malaria?
    Sue Adams
    Malaria Research Group, Nuffield Dept of Clinical Laboratory Sciences, Oxford Wellcome Centre for Tropical and Infectious Diseases, John Radcliffe Hospital, Oxford, UK OX3 9DU
    Trends Parasitol 18:360-6. 2002
    ..Understanding how this happens and how it contributes to the pathogenesis of coma may provide new opportunities for the treatment of cerebral malaria...
  72. ncbi Focal adhesion kinase: in command and control of cell motility
    Satyajit K Mitra
    The Scripps Research Institute, Department of Immunology, IMM21 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 6:56-68. 2005
    ..Through multifaceted and diverse molecular connections, FAK can influence the cytoskeleton, structures of cell adhesion sites and membrane protrusions to regulate cell movement...
  73. ncbi Isometric contraction of Dupuytren's myofibroblasts is inhibited by blocking intercellular junctions
    Jennifer S N Verhoekx
    1 Kennedy Institute of Rheumatology, University of Oxford, London, UK 2 Department of Plastic and Reconstructive Surgery, Erasmus Medical Centre, Rotterdam, The Netherlands
    J Invest Dermatol 133:2664-71. 2013
    ..Therefore, we studied intercellular junctions of primary human MFs derived from patients with Dupuytren's disease, a fibrotic disorder of the dermis ..
  74. ncbi Protecting your tail: regulation of cadherin degradation by p120-catenin
    Andrew P Kowalczyk
    Departments of Dermatology and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Curr Opin Cell Biol 16:522-7. 2004
    ....
  75. ncbi Beyond the gap: functions of unpaired connexon channels
    Daniel A Goodenough
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Nat Rev Mol Cell Biol 4:285-94. 2003
    ..Recent studies indicate that connexons are also active in single plasma membranes and that they might be essential in intercellular signalling beyond their incorporation into gap junctions...
  76. ncbi Biology and pathology of nectins and nectin-like molecules
    Toshiaki Sakisaka
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Osaka 565 0871, Japan
    Curr Opin Cell Biol 16:513-21. 2004
    ..On the other hand, nectins and Necls serve as viral receptors and are associated with human diseases (including cancer) when mutated or upregulated...
  77. ncbi Role of Lgl/Dlg/Scribble in the regulation of epithelial junction, polarity and growth
    Tomoyuki Yamanaka
    Laboratory for Structural Neuropathology, RIKEN Brain Science Institute, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Front Biosci 13:6693-707. 2008
    ....
  78. pmc Defects in nuclear and cytoskeletal morphology and mitochondrial localization in spermatozoa of mice lacking nectin-2, a component of cell-cell adherens junctions
    M J Bouchard
    Departments of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Mol Cell Biol 20:2865-73. 2000
    ..The structural defects observed in spermatozoa of nectin-2(-/-) mice suggest a role for this protein in organization and reorganization of the cytoskeleton during spermiogenesis...
  79. pmc A mechanism of Rap1-induced stabilization of endothelial cell--cell junctions
    Jian J Liu
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 22:2509-19. 2011
    ..These studies establish that the direct physical interaction of Rap1 with KRIT1 enables the translocation of microtubule-sequestered KRIT1 to junctions, thereby supporting junctional integrity and cardiovascular development...
  80. pmc The keratin-binding protein Albatross regulates polarization of epithelial cells
    Masahiko Sugimoto
    Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya 464 8681, Japan
    J Cell Biol 183:19-28. 2008
    ..We propose that keratins and the keratin-binding protein Albatross are important for epithelial cell polarization...
  81. pmc Ectoplasmic specialization: a friend or a foe of spermatogenesis?
    Helen H N Yan
    Center for Biomedical Research, Population Council, New York, NY 10021, USA
    Bioessays 29:36-48. 2007
    ..We discuss herein the mechanisms that maintain the cross-talk between ES and blood-testis barrier to facilitate cell movement and orientation in the seminiferous epithelium...
  82. ncbi Reprogramming of cell junction modules during stepwise epithelial to mesenchymal transition and accumulation of malignant features in vitro in a prostate cell model
    Xi Song Ke
    The Gade Institute, University of Bergen, Bergen, Norway
    Exp Cell Res 317:234-47. 2011
    ..Reprogramming of cell junction modules is involved in both steps...
  83. ncbi Congenital nephrotic syndrome in mice lacking CD2-associated protein
    N Y Shih
    Center for Immunology and Department of Pathology, Washington University, Saint Louis, MO 63110, USA
    Science 286:312-5. 1999
    ..Supporting a role for CD2AP in the specialized cell junction known as the slit diaphragm, CD2AP associated with nephrin, the primary component of the slit diaphragm...
  84. pmc Mouse middle ear ion homeostasis channels and intercellular junctions
    Lisa M Morris
    Oregon Hearing Research Center, Department of Otolaryngology Head and Neck Surgery, Oregon Health and Science University, Portland, Oregon, United States of America
    PLoS ONE 7:e39004. 2012
    ..The middle ear contains homeostatic mechanisms that control the movement of ions and fluids similar to those present in the inner ear, and are altered during inflammation...
  85. pmc Disruption of Nfic causes dissociation of odontoblasts by interfering with the formation of intercellular junctions and aberrant odontoblast differentiation
    Tae Yeon Lee
    Department of Conservative Dentistry and Dental Research Institute, College of Dentistry, Seoul National University, 28 Yeon Gun Dong, Jong Ro Gu, Seoul 110 749, Korea
    J Histochem Cytochem 57:469-76. 2009
    ..The most striking changes observed in these aberrant odontoblasts were the loss of intercellular junctions and the decreased expression of ZO-1 and occludin...
  86. ncbi Molecular characterization and spatial distribution of SAP97, a novel presynaptic protein homologous to SAP90 and the Drosophila discs-large tumor suppressor protein
    B M Müller
    Center for Molecular Neurobiology, University of Hamburg, Germany
    J Neurosci 15:2354-66. 1995
    ..These data suggest that members of the SAP90/SAP97 subfamily may be involved in the site specific assembly, stability or functions of membrane specialization at sites of cell-cell contact...
  87. ncbi Role of transglutaminase 1 in stabilisation of intercellular junctions of the vascular endothelium
    Werner Baumgartner
    Institute of Anatomy and Cell Biology, University of Wurzburg, Koellikerstrasse 6, 97070 Wurzburg, Germany
    Histochem Cell Biol 122:17-25. 2004
    ..of MyEnd cells to barrier-breaking conditions correlated strongly with translocation of TGase1 to intercellular junctions. Simultaneous inhibition of intracellular and extracellular TGase activity by monodansylcadaverine (MDC) ..
  88. ncbi A niche maintaining germ line stem cells in the Drosophila ovary
    T Xie
    Department of Embryology, Howard Hughes Medical Institute, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
    Science 290:328-30. 2000
    ..Our studies implicate one of the somatic cell types, the cap cells, as a key niche component...
  89. ncbi Epidermal growth factor induces tyrosine phosphorylation and reorganization of the tight junction protein ZO-1 in A431 cells
    C M Van Itallie
    Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520 8019, USA
    J Cell Sci 108:1735-42. 1995
    ..polarized epithelial cells, ZO-1 is restricted to the cytoplasmic surface of the most apical of the intercellular junctions, the tight junction...
  90. ncbi The cell junction protein VAB-9 regulates adhesion and epidermal morphology in C. elegans
    Jeffrey S Simske
    Department of Zoology, University of Wisconsin, Madison, 1117 West Johnson Street, Madison, WI 53706 USA
    Nat Cell Biol 5:619-25. 2003
    ..Thus, VAB-9 represents the first characterized tetraspan adherens junction protein in C. elegans and defines a new family of such proteins in higher eukaryotes...
  91. pmc Molecular mechanism for orienting membrane and actin dynamics to nascent cell-cell contacts in epithelial cells
    Marc D H Hansen
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305 5426, USA
    J Biol Chem 277:45371-6. 2002
    ..This shift may reflect a diffusion trapping mechanism by which these Rac1 complexes are localized to cadherin-mediated cell-cell contacts through an interaction with annexin II...
  92. ncbi Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts
    Pipsa Saharinen
    Molecular Cancer Biology Laboratory and Ludwig Institute for Cancer Research, Biomedicum Helsinki, University of Helsinki, Haartmaninkatu 8, P O B 63, 00014 Helsinki, Finland
    Nat Cell Biol 10:527-37. 2008
    ..This novel type of cellular microenvironment-dependent receptor tyrosine kinase activation may explain some of the effects of angiopoietins in angiogenesis and vessel stabilization...
  93. pmc EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2
    Jodi L Klessner
    Departments of Pathology and Dermatology, and the R H Lurie Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
    Mol Biol Cell 20:328-37. 2009
    ..Here, we show that EGFR inhibition stabilizes Dsg2 at intercellular junctions by interfering with its accumulation in an internalized cytoplasmic pool...
  94. ncbi Mesenchymal-epithelial transitions: spontaneous and cumulative syntheses of epithelial marker molecules and their assemblies to novel cell junctions connecting human hematopoietic tumor cells to carcinomatoid tissue structures
    Werner W Franke
    Helmholtz Group for Cell Biology, German Cancer Research Center, Heidelberg, Germany
    Int J Cancer 129:2588-99. 2011
    ..We discuss molecular mechanisms involved in the formation and function of these junctions, also with respect to tumor spread and metastasis, as well as diagnostic and therapeutic consequences...
  95. pmc Establishment and characterization of a novel untransfected corneal endothelial cell line from New Zealand white rabbits
    Tingjun Fan
    Research Institute of Corneal Tissue Engineering, Ocean University of China, Shandong, China
    Mol Vis 15:1070-8. 2009
    ..To establish and characterize a novel untransfected corneal endothelial cell line from New Zealand white rabbits (NRCE cell line) for studies on corneal endothelial cells...
  96. pmc The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions
    E Willott
    Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510
    Proc Natl Acad Sci U S A 90:7834-8. 1993
    ..These results suggest a protein family specialized for signal transduction on the cytoplasmic surface of intercellular junctions. These results also provide biochemical evidence for similarity between invertebrate septate and ..
  97. ncbi Dynamic regulation of epithelial cell fate and barrier function by intercellular junctions
    Stefan Koch
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA
    Ann N Y Acad Sci 1165:220-7. 2009
    ..Epithelial barrier function is provided by tightly regulated intercellular junctions, which consist of a plethora of membrane-associated and transmembrane proteins organized in discreet, ..
  98. ncbi Spatial organization of signal transduction molecules in the NK cell immune synapses during MHC class I-regulated noncytolytic and cytolytic interactions
    Y M Vyas
    Immunology Program, Sloan-Kettering Institute for Cancer Research, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA
    J Immunol 167:4358-67. 2001
    ..We also demonstrate, in three-cell conjugates, that a single NK cell forms a cytolytic synapse with a susceptible target cell in the presence of both susceptible and nonsusceptible target cells...
  99. pmc Peripheral myelin protein 22 is a constituent of intercellular junctions in epithelia
    L Notterpek
    Department of Neuroscience, McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 98:14404-9. 2001
    ..These studies support a role for PMP22 at intercellular junctions of epithelia and may indicate a similar function in myelinating Schwann cells...
  100. ncbi Endocytosis of the apical junctional complex: mechanisms and possible roles in regulation of epithelial barriers
    Andrei I Ivanov
    Epithelial Pathobiology Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Room 115, 615 Michael Street, Atlanta, GA 30322, USA
    Bioessays 27:356-65. 2005
    ..Potential biological roles for AJC endocytosis in maintaining functional apical junctions, reversible opening of epithelial barrier and disruption of intercellular adhesion are also discussed...
  101. ncbi LET-23 receptor localization by the cell junction protein LIN-7 during C. elegans vulval induction
    J S Simske
    Department of Developmental Biology, Stanford University Medical School, California 94305 5427, USA
    Cell 85:195-204. 1996
    ..These results suggest that proper localization of LET-23 receptor to the Pn.p cell junctions is required for signaling activity...

Research Grants62

  1. Connexins in Neuronal and Glial Gap Junctions in the Central Nervous System
    John E Rash; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Gap junctions are microscopic intercellular junctions that provide for direct intercellular ionic, electrical, and metabolic coupling between nerve cells in the brain, retina, and spinal cord...
  2. MECHANISM OF ENDOTOXIN ABSORPTION IN ALCOHOLISM
    Radhakrishna Rao; Fiscal Year: 2013
    ..acetaldehyde disrupts intestinal epithelial barrier function by inducing phosphorylation of proteins of intercellular junctions, and the probiotic L. plantarum prevents such cellular damage by acetaldehyde...
  3. Structure and Function of Endothelial Fenestrae
    RADU VIRGIL STAN; Fiscal Year: 2010
    ..specific organelles such as transendothelial channels, fenestrae, vesiculo-vacuolar organelles as well as intercellular junctions), with the exception of caveolae and the intercellular junctions, there is very little to no biochemical ..
  4. STUDIES ON OVARIAN RING CANALS IN DROSOPHILA
    Lynn Cooley; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Ring canals are stable intercellular junctions that form cytoplasmic bridges between cells...
  5. Cutaneous wound remodeling by re-differentiating keratinocytes expressing CXCR3 l
    ARTHUR HUEN; Fiscal Year: 2013
    ..In the second aim, the hypothesis that re-establishment of intercellular junctions by keratinocytes is a trigger for chemokine expression will be examined...
  6. Contribution of occludin to intestinal homeostasis and disease
    Le Shen; Fiscal Year: 2013
    ..The epithelial barrier is maintained by the continuous layer of epithelial cells and the intercellular junctions, primarily the tight junction, that join them...
  7. Engineering of Human Corneal Endothelial Grafts
    Ying Ting Zhu; Fiscal Year: 2012
    ..signaling, our novel engineering strategy may control cellular proliferation without disrupting their intercellular junctions, hence avoiding both the use of enzymatically dissociated single cells and the risk of losing the normal ..
  8. Molecular Biology and Function of PECAM-1
    Peter J Newman; Fiscal Year: 2013
    ..an inhibitory receptor on platelets and leukocytes, and is also a prominent component of endothelial cell intercellular junctions. The goal of this proposal is to improve our understanding of the function of this novel vascular cell ..
  9. Cytoskeletal regulation of intestinal barrier function and response to pathogens
    John Garber; Fiscal Year: 2013
    ..how factors that regulate the actin cytoskeleton influence the stability and dynamic function of intercellular junctions within the intestinal epithelium...
  10. PROTEINS OF THE ENDOTHELIAL CELL SURFACE
    Jordan S Pober; Fiscal Year: 2013
    ..Surface proteins localized to the intercellular junctions of ECs control vascular permeability...
  11. CATENINS, CADHERINS AND THEIR INTERACTIONS
    William I Weis; Fiscal Year: 2012
    The assembly of cells into tissues depends upon formation of intercellular junctions. In adherens junctions, the catenins link transmembrane cadherin cell adhesion molecules to the actin-based cytoskeleton, and in desmosomes an ..
  12. TRANSPORT ACROSS CAPILLARY ENDOTHELIUM
    Fitz Roy E Curry; Fiscal Year: 2013
    ..do not respond to thrombin and permeability is low because strong adhesion forces maintain tight intercellular junctions. We propose experiments to induce the contractile phenotype in microvessels and tissues, which initially ..
  13. Role of cell adhesion in organizing membrane growth.
    CHARLES A YEAMAN; Fiscal Year: 2013
    ..of intercellular contact, and subsequently move apart to facilitate the development of different types of intercellular junctions. The specific aims of this study are: 1) to investigate the function of Ral-Exocyst complexes in ..
  14. In vivo mechanisms for epithelial cell polarization
    STEPHEN THOMAS ARMENTI; Fiscal Year: 2013
    ..One of the earliest events in epithelial differentiation is the formation of intercellular junctions required for cell-cell adhesion, barrier function and apicobasal polarity...
  15. Role of the Na K ATPase Beta 1 subunit in alveolar epithelial integrity
    Olga Vagin; Fiscal Year: 2013
    ..regulatory subunits of the Na,K- ATPase activity, which is also implicated in the impairment of intercellular junctions. In preliminary experiments, we observed that hypoxia impairs intercellular adhesion and up-regulates ..
  16. Cadherin Mediated Cell-Cell Interactions in the Bone Microenvironment
    Roberto Civitelli; Fiscal Year: 2013
    ..cadherin deficiency negatively affects the Wnt/[unreadable]-catenin system and reduces the abundance of intercellular junctions (adherens and gap junctions), in vitro...
  17. Mechanisms of Aqueous Humor Homeostasis -- Role of Monocytes
    Jorge A Alvarado; Fiscal Year: 2013
    ..to envision the role of intracellular signaling pathways, and important mediators including cytokines and intercellular junctions. The model specifically considers the role of monocytes and monocyte secreted cytokines...
  18. ESOPHAGEAL CYTOPROTECTION--AGENTS AND MECHANISMS
    Roy Orlando; Fiscal Year: 2009
    ..In this grant, we focus on the barrier properties of the EE, and especially that of the intercellular junctions (ICJs)...
  19. FUNCTION OF NEUREXINS
    Thomas C Sudhof; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Synapses are intercellular junctions that mediate neuronal communication, and likely involve many cell-adhesion molecules that connect pre- and postsynaptic neurons to each other...
  20. Characterizing the role of male circumcision in HIV sexual transmission
    MINH HIEN TRUONG DINH; Fiscal Year: 2013
    ..Tissue will be studied for target cells, keratin, and intercellular junctions. Long-term tissue explants inoculated with SIV GFP-Vpx constructs will be used to study early SIV ..
  21. Integrating cells into the auditory epithelium of deaf ears
    Yehoash Raphael; Fiscal Year: 2013
    ..and test extent of integration of cells inserted into the endolymph while potassium levels are low and intercellular junctions are open (Aim 3)...
  22. Modulating the BBB to Improve Drug Delivery to the Brain
    Teruna J Siahaan; Fiscal Year: 2013
    ..modulating the adherens junction of the BBB using E-cadherin peptides can enhance the porosity of the intercellular junctions and, thus, enhance the paracellular permeation of small and large anticancer drugs...
  23. Intestinal Epithelial Tight Junction Structure-Function
    Asma Nusrat; Fiscal Year: 2013
    ..Epithelial barrier function is regulated by a series of intercellular junctions that encompass the tight junction (TJ), adherens junction (AJ) and desmosomes (DMs)...
  24. LOCATION OF PORE SYSTEMS IN CAPILLARY WALLS
    George Palade; Fiscal Year: 2000
    ..the concept/process of transcytosis in the handling of macromolecules in continuous endothelia bounded by intercellular junctions. Morphological and cell fractionation methods will be employed to study the entrance and exit of the ..
  25. Desmoglein 2 Function in Cell Adhesion and Pemphigus
    MY MAHONEY; Fiscal Year: 2003
    Epidermal keratinocytes are held together by intercellular junctions called desmosomes, which are critical for the maintenance of cell-cell adhesion, tissue integrity, and organ function...
  26. Asymmetric flow FFF and Composition Gradient/Light Scattering System
    Ewa Folta Stogniew; Fiscal Year: 2007
    ..involved in acute renal injury or intestinal inflammation, dopamine transporters, prolactin receptor, intercellular junctions, remodeling complexes at locus control regions, nuclear pore complex, infection by group A streptococci, ..
  27. MALE REPRODUCTIVE TOXICITY AND ESTROGENICITY OF PAHS
    Samir Raychoudhury; Fiscal Year: 1999
    ..SPECIFIC AIM D: To determine the effects of PAHs exposure on specific intercellular junctions of the seminiferous epithelium...
  28. Communication Between Laminin 5 and Gap Junctions
    William Carter; Fiscal Year: 2005
    ..Cell-cell interactions are mediated by at least three types of intercellular junctions: adherens junctions (AJs), desmosomes and gap junctions (GJs)...
  29. Modulation of the Intercellular Junction Cadherins
    Teruna Siahaan; Fiscal Year: 2001
    The long-term objective of this project is to understand how to modulate tight intercellular junctions for improving drug delivery by regulating protein interactions that mediate the intercellular junctions...
  30. MODULATION OF GENE EXPRESSION IN CELL CULTURE
    RODY COX; Fiscal Year: 1980
    ..The morphological differentiation consists of the development of specialized intercellular junctions - desmosomes and the organization of microfilaments into tonofilaments which resemble those found in ..
  31. Adherens Junction Integrity and Barrier Function
    Fred Minnear; Fiscal Year: 2005
    ..We propose that the barrier-enhancing activity of S1P and cAMP is directed at the intercellular junctions. Alternatively, S1P and cAMP may function via different mechanisms...
  32. GROWTH FACTOR MODULATION OF REEPITHELIALIZATION
    LAURIE HUDSON; Fiscal Year: 2004
    ..have been reported to disappear during the migratory phase of reepithelialization and reassembly of these intercellular junctions marks the end of keratinocyte migration; however, the mechanisms underlying dynamic regulation of ..
  33. CD11B/CD18 AND NEUTROPHIL EPITHELIAL INTERACTIONS
    CHARLES PARKOS; Fiscal Year: 2003
    ..superfamily member termed junction adhesion molecule (JAM) that is concentrated at cell-to-cell borders (intercellular junctions) of murine endothelium and epithelium was shown to participate in monocyte transmigration across mouse ..
  34. MODULATION OF THE ENDOTHELIUM BY THROMBIN AND SHEAR
    Marilyn Woolkalis; Fiscal Year: 2004
    ..VE-cadherin complexes, which form the calcium-dependent intercellular junctions of endothelial cells, are targets of signaling pathways activated by thrombin receptors and fluid shear ..
  35. INTESTINAL MUCOSAL WOUND RESEALING IN SURGICAL DISEASES
    Asma Nusrat; Fiscal Year: 2002
    ..extensions at the leading edge and is dependent on dynamic cytoskeletal remodeling with modification of intercellular junctions. To test this hypothesis experiments have been proposed utilizing an in vitro model of wound resealing...
  36. IN VITRO MODEL OF THE BLOOD-BRAIN BARRIER
    Luz Claudio; Fiscal Year: 2001
    ..molecule that initially forces the plasma membranes into close apposition and then triggers formation of intercellular junctions. Previous experiments in HeLa cells, a carcinoma cell line, and preliminary experiments in endothelial ..
  37. Blood-brain barrier traversal by African trypanosomes
    DENNIS GRAB; Fiscal Year: 2006
    ..b. gambiense parasites bind at or near intercellular junctions and cross the in vitro BBB model paracellularly more efficiently than do animal infective T. b. brucei...
  38. TRANSMISSION ELECTRON MICROSCOPE
    Barry Timms; Fiscal Year: 1992
    ..and morphology of lung tissue in animal models of pulmonary edema, in addition to examining the intercellular junctions and integrity of monolayer cultures of pulmonary alveolar epithelium. Dr...
  39. MRI Measure of Blood Brain Barrier Permeability
    James Ewing; Fiscal Year: 2006
    ..barrier is a unique feature of cerebral vasculature, characterized at the capillary level by tight intercellular junctions devoid of fenestrae, and isolating the brain from the rest of the body...
  40. Regulation Epithelial Cell Growth by the Tight Junction
    Vivian Tang; Fiscal Year: 2006
    ..because they retain key functional characteristics of the intestinal epithelium including formation of intercellular junctions, generation of a polarized phenotype, exhibition of contact-inhibition of growth, responsiveness to ..
  41. CELLULAR BIOLOGY OF BRAIN MICROVESSELS
    Sami Harik; Fiscal Year: 1992
    ..To accomplish this, the brain capillary endothelium is endowed with unique features, such as tight intercellular junctions, transporters for essential water-soluble molecules such as glucose and amino acids, and enzyme system ..
  42. PECAM AND CALCIUM SIGNALING AND TRANSMIGRATION
    Steven Albelda; Fiscal Year: 2002
    ..PECAM-1) is a member of the immunoglobulin superfamily that is expressed at high levels on endothelial intercellular junctions and regulates transendothelial migration of leukocytes through unknown mechanisms...
  43. CADHERIN DYNAMICS AND GLAUCOMA
    W Daniel Stamer; Fiscal Year: 2010
    ..extracellular matrix materials of the juxtacanalicular tissue;or (ii) alterations in the function of the intercellular junctions (and associated border pores) of the inner wall of Schlemm's canal...
  44. INTERACTION OF ENDOTHELIAL CELLS AND LEUKOCYTES
    Samuel Silverstein; Fiscal Year: 1993
    ..The cells exhibit many of the ultrastructural and functional properties of endothelium in vivo. Their intercellular junctions stain with silver; they exhibit electron dense plaques characteristic of tight junctions at zones of ..
  45. REGULATION OF CELL-TO-CELL CHANNELS IN THE LENS
    ROSS JOHNSON; Fiscal Year: 1990
    The fiber cells in the vertebrate lens are connected by unusually extensive intercellular junctions. It has been suggested that these junctions contain cell-to-cell channels, based on demonstrations of the intercellular movement of small ..
  46. EBV BMRF-2 Protein in Infection of Oral Epithelial Cells
    Sharof Tugizov; Fiscal Year: 2007
    ..progeny virions to the basolateral membrane and may facilitate translocation of virions across lateral intercellular junctions. The goal of this study is to characterize molecular mechanisms of BMRF-2/beta1 integrin interactions and ..
  47. INTERCELLULAR JUNCTIONS
    BRUCE STEVENSON; Fiscal Year: 2000
    The complex mechanisms of cell-cell interaction, as mediated by intercellular junctions, are now being understood at the molecular level...
  48. Intestinal Barrier Function and Type 1 Diabetes
    Josef Neu; Fiscal Year: 2005
    ..that the nature of this permeability defect relates to differences in the proteins of the intestinal intercellular junctions. Manipulation of the junctional defect by specific nutrients holds exciting promise in the prevention of ..
  49. Functions of the Epithelial Septate Junction
    Richard Fehon; Fiscal Year: 2009
    ..b>Intercellular junctions, including adherens, tight, and septate junctions, have well-defined structural functions in ..
  50. DERMAL ENDOTHELIAL INTERCELLULAR JUNCTIONS
    Andrew Kowalczyk; Fiscal Year: 2001
    ..area of research focusing on the molecular and cellular biology of dermal microvascular endothelial intercellular junctions. The research will be carried out at Northwestern University Medical School where the faculty have ..
  51. P71 IN DERMAL MICROVASCULAR ENDOTHELIAL JUNCTION ASSEMBL
    Andrew Kowalczyk; Fiscal Year: 2001
    ..Adhesive intercellular junctions that form between adjacent endothelial cells are thought to play crucial roles in endothelial barrier ..
  52. PECAM-1'S INVOLVEMENT IN ANGIOGENESIS
    Horace Delisser; Fiscal Year: 2002
    ..One important cell adhesion molecule on endothelial cells is PECAM-1 where it concentrates at intercellular junctions. Recently, data has implicated PECAM-1 in in vivo angiogenesis...
  53. Cellular and Molecular Mechanisms of Vascular Permeability
    RADU STAN; Fiscal Year: 2009
    ..specific organelles such as transendothelial channels, fenestrae, vesiculo-vacuolar organelles as well as intercellular junctions), there is very little to no biochemical evidence on the molecular composition of the structures involved,..
  54. Molecular & functional aspects of vascular permeability
    RADU STAN; Fiscal Year: 2004
    ..e. caveolae, transendothelial channels, fenestrae and intercellular junctions), there is very little to no biochemical evidence on the molecular composition of the structures involved,..
  55. Ethanol, Tat and dysfunction of brain endothelium
    Michal Toborek; Fiscal Year: 2004
    ..stress can lead to decreased expression of specific transmembrane proteins, such as occludin, which build intercellular junctions, i.e., the structures responsible for the integrity of the brain endothelium and functional BBB...
  56. Characterization of G Protein, Rap1, and its Role in Prostate Cancer
    VERONICA HENDERSON; Fiscal Year: 2009
    ..This process results in breakdown of intercellular junctions which results to increased cell migratory potential...
  57. INTERLEUKIN 6 AND HUMAN BILIARY EPITHELIAL PROLIFERATION
    Anthony Demetris; Fiscal Year: 2006
    ..matrix and immunosuppressive drugs on biliary epithelial cell mitogenesis, apoptosis and maintenance of intercellular junctions, in vitro. b...
  58. BIOMEDICAL RESEARCH SUPPORT GRANT
    Linda Phillips; Fiscal Year: 1991
    ..These projects involve the plasma membrane and intercellular junctions, endosomes, the endoplasmic reticulum and membrane trafficking, interphase nd mitotic microtubule arrays, ..
  59. CELL ADHESION IN DEVELOPMENT OF THE EYE
    Urs Rutishauser; Fiscal Year: 1999
    ..addressed in this proposal are the migration and positioning of cells, the formation of specialized intercellular junctions, and promotion of target-dependent changes in neuronal biochemistry...
  60. Chemokine Receptor Chimeras by Synthetic Gene Library
    Michael Dustin; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  61. An in vivo model to study blood-testis barrier dynamics
    C Yan Cheng; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  62. Junction Dynamics and Male Fertility Regulation
    C Yan Cheng; Fiscal Year: 2008
    ..In summary, these studies not only will define the regulation of AJ dynamics, they will identify new leads and innovative approaches for male contraceptive development. [unreadable] [unreadable]..