bacterial fimbriae

Summary

Summary: Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).

Top Publications

  1. ncbi Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili
    L A Pratt
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
    Mol Microbiol 30:285-93. 1998
  2. ncbi Type IV pili and twitching motility
    John S Mattick
    ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
    Annu Rev Microbiol 56:289-314. 2002
  3. ncbi Type IV pilus structure and bacterial pathogenicity
    Lisa Craig
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Rev Microbiol 2:363-78. 2004
  4. pmc Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek
    Sean Paul Nuccio
    Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, One Shields Ave, Davis, CA 95616 8645, USA
    Microbiol Mol Biol Rev 71:551-75. 2007
  5. ncbi Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation
    Anton V Zavialov
    Department of Molecular Biosciences Structural Biology, Uppsala Biomedical Center, Swedish University of Agricultural Sciences, Box 590, SE 751 24 Uppsala, Sweden
    Cell 113:587-96. 2003
  6. ncbi X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli
    D Choudhury
    Department of Molecular Biology, Uppsala Biomedical Center, Swedish University of Agricultural Sciences, Box 590, S 753 24 Uppsala, Sweden
    Science 285:1061-6. 1999
  7. pmc Direct observation of extension and retraction of type IV pili
    J M Skerker
    Rowland Institute for Science, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 98:6901-4. 2001
  8. ncbi Structural basis of chaperone function and pilus biogenesis
    F G Sauer
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Science 285:1058-61. 1999
  9. ncbi Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions
    Lisa Craig
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
    Mol Cell 23:651-62. 2006
  10. ncbi Complete genome sequence of Salmonella enterica serovar Typhimurium LT2
    M McClelland
    Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, California 92121, USA
    Nature 413:852-6. 2001

Research Grants

Detail Information

Publications248 found, 100 shown here

  1. ncbi Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili
    L A Pratt
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
    Mol Microbiol 30:285-93. 1998
    ..In light of the observations presented here, a working model is discussed that describes the roles of both motility and type I pili in biofilm development...
  2. ncbi Type IV pili and twitching motility
    John S Mattick
    ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
    Annu Rev Microbiol 56:289-314. 2002
    ..Twitching motility is also controlled by a range of signal transduction systems, including two-component sensor-regulators and a complex chemosensory system...
  3. ncbi Type IV pilus structure and bacterial pathogenicity
    Lisa Craig
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Rev Microbiol 2:363-78. 2004
  4. pmc Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek
    Sean Paul Nuccio
    Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, One Shields Ave, Davis, CA 95616 8645, USA
    Microbiol Mol Biol Rev 71:551-75. 2007
    ..The proposed classification system offers a simple and convenient method for assigning newly discovered chaperone/usher systems to one of the six major phylogenetic groups...
  5. ncbi Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation
    Anton V Zavialov
    Department of Molecular Biosciences Structural Biology, Uppsala Biomedical Center, Swedish University of Agricultural Sciences, Box 590, SE 751 24 Uppsala, Sweden
    Cell 113:587-96. 2003
    ..The observed conformational change suggests that the chaperone traps a high-energy folding intermediate of Caf1. A model is proposed in which release of the subunit allows folding to be completed, driving fiber formation...
  6. ncbi X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli
    D Choudhury
    Department of Molecular Biology, Uppsala Biomedical Center, Swedish University of Agricultural Sciences, Box 590, S 753 24 Uppsala, Sweden
    Science 285:1061-6. 1999
    ..A donor strand complementation mechanism in which the chaperone donates a strand to complete the pilin domain explains the basis for both chaperone function and pilus biogenesis...
  7. pmc Direct observation of extension and retraction of type IV pili
    J M Skerker
    Rowland Institute for Science, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 98:6901-4. 2001
    ..Occasionally, cell bodies came free and were pulled forward by pilus retraction. Thus, type IV pili are linear actuators that extend, attach at their distal tips, exert substantial force, and retract...
  8. ncbi Structural basis of chaperone function and pilus biogenesis
    F G Sauer
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Science 285:1058-61. 1999
    ..The structure of the PapD-PapK complex also suggests that during pilus biogenesis, every subunit completes the immunoglobulin-like fold of its neighboring subunit via a mechanism termed donor strand exchange...
  9. ncbi Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions
    Lisa Craig
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
    Mol Cell 23:651-62. 2006
    ..These results clarify T4P multifunctionality and assembly-disassembly while suggesting unified assembly mechanisms for T4P, archaeal flagella, and type II secretion system filaments...
  10. ncbi Complete genome sequence of Salmonella enterica serovar Typhimurium LT2
    M McClelland
    Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, California 92121, USA
    Nature 413:852-6. 2001
    ..Most of these homologues were previously unknown, and 50 may be exported to the periplasm or outer membrane, rendering them accessible as therapeutic or vaccine targets...
  11. pmc Single pilus motor forces exceed 100 pN
    Berenike Maier
    Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, New York, NY 10027, USA
    Proc Natl Acad Sci U S A 99:16012-7. 2002
    ..The results are most consistent with a model in which the ATPase PilT forms an oligomer that disassembles the pilus by a cooperative conformational change...
  12. ncbi Intracellular bacterial biofilm-like pods in urinary tract infections
    Gregory G Anderson
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Science 301:105-7. 2003
    ..The discovery of intracellular biofilm-like pods explains how bladder infections can persist in the face of robust host defenses...
  13. ncbi Development of intracellular bacterial communities of uropathogenic Escherichia coli depends on type 1 pili
    Kelly J Wright
    Department of Molecular Microbiology, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Cell Microbiol 9:2230-41. 2007
    ..Thus, this work changes the extracellular colonization functional paradigm of pili by demonstrating their intracellular role in biofilm formation...
  14. pmc Salmonella enterica serovar Typhi possesses a unique repertoire of fimbrial gene sequences
    S M Townsend
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University, College Station, Texas 77843, USA
    Infect Immun 69:2894-901. 2001
    ..However, the serotype Typhi genome differed from that of all other Salmonella serotypes investigated in that it contained a unique combination of putative fimbrial operons...
  15. ncbi Assembly of pili on the surface of Corynebacterium diphtheriae
    Hung Ton-That
    Committee on Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Mol Microbiol 50:1429-38. 2003
    ..This covalent tethering of adjacent pilin subunits appears to have evolved in many Gram-positive pathogens that encode sortase and pilin subunit genes with sorting signals and pilin motifs...
  16. ncbi Streptococcus pneumoniae contains 3 rlrA pilus variants that are clonally related
    Monica Moschioni
    Novartis Vaccines, Siena, Italy
    J Infect Dis 197:888-96. 2008
    ..Limited data are available on the prevalence of the rlrA operon in pneumococci; therefore, we investigated its distribution and its antigenic variation among disease-causing strains...
  17. ncbi Assembly of pili in Gram-positive bacteria
    Hung Ton-That
    Committee on Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Trends Microbiol 12:228-34. 2004
  18. pmc Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria
    Luciano A Marraffini
    Department of Microbiology, Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Microbiol Mol Biol Rev 70:192-221. 2006
    ..This review focuses on the mechanisms of surface protein anchoring to the cell wall envelope by sortases and the role that these enzymes play in bacterial physiology and pathogenesis...
  19. ncbi Stabilizing isopeptide bonds revealed in gram-positive bacterial pilus structure
    Hae Joo Kang
    Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland 1010, New Zealand
    Science 318:1625-8. 2007
    ..The present structure explains the strength and stability of such Gram-positive pili and could facilitate vaccine development...
  20. pmc The Myxococcus xanthus pilQ (sglA) gene encodes a secretin homolog required for type IV pilus biogenesis, social motility, and development
    D Wall
    Departments of Biochemistry and Developmental Biology, Stanford University, Stanford, California 94305
    J Bacteriol 181:24-33. 1999
    ..Many differences between pilQ1 and pilQ+ strains have been noted in the literature. We discuss some of these observations and how they may be rationalized in the context of our molecular and functional findings...
  21. pmc Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility
    Kenneth A Satyshur
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Structure 15:363-76. 2007
    ..We present a model for ATP binding leading to dramatic PilT domain motions, engagement of the arginine wire, and subunit communication in this hexameric motor. Our conclusions apply to the entire type II/IV secretion ATPase family...
  22. ncbi Exploring the 3D molecular architecture of Escherichia coli type 1 pili
    Erik Hahn
    Institute of Veterinary Anatomy, University of Zurich, Switzerland
    J Mol Biol 323:845-57. 2002
    ....
  23. pmc Type IV pili: paradoxes in form and function
    Lisa Craig
    Molecular Biology and Biochemistry Department, Simon Fraser University, 8888 University Dr, Burnaby, BC, Canada V5A 1S6
    Curr Opin Struct Biol 18:267-77. 2008
    ..This review will summarize the most recent structural advances on Type IV pili and their assembly components and highlight their significance...
  24. pmc A sticky chain model of the elongation and unfolding of Escherichia coli P pili under stress
    Magnus Andersson
    Department of Physics, Umea University, SE 901 87 Umea, Sweden
    Biophys J 90:1521-34. 2006
    ..Entities such as the free energy, the stiffness, the elastic elongation, the opening length of the various bonds, and the number of PapA units in the rod are determined...
  25. pmc Salmonella enterica serovar Typhimurium requires the Lpf, Pef, and Tafi fimbriae for biofilm formation on HEp-2 tissue culture cells and chicken intestinal epithelium
    Nathan A Ledeboer
    Department of Microbiology, Roy J and Lucille A Carver School of Medicine, University of Iowa, Iowa City, IA 52242 1109
    Infect Immun 74:3156-69. 2006
    ..An understanding of these interactions is likely to provide new insights for intervention strategies in Salmonella colonization and infection...
  26. ncbi Expression of cellulose and curli fimbriae by Escherichia coli isolated from the gastrointestinal tract
    Werner Bokranz
    Department of Cell Biology and Immunology, Gesellschaft fur Biotechnologische Forschung, 38124 Braunschweig, Germany
    J Med Microbiol 54:1171-82. 2005
    ..In contrast to S. enterica, at 37 degrees C pellicle formation occurred in the absence of cellulose and curli fimbriae. The gastrointestinal tract represents a source of biofilm-forming bacteria, which can spread to susceptible sites...
  27. ncbi Weapons of mass retraction
    Lori L Burrows
    Department of Surgery, University of Toronto and Programme in Infection, Immunity, Injury and Repair, Hospital for Sick Children Research Institute, Toronto, ON, Canada
    Mol Microbiol 57:878-88. 2005
    ..This review focuses on recent discoveries relating to the assembly and function of T4P in generation of twitching motility...
  28. ncbi Identification of Escherichia coli O157 : H7 genes influencing colonization of the bovine gastrointestinal tract using signature-tagged mutagenesis
    Francis Dziva
    Mammalian Enteric Pathogens Group, Division of Microbiology, Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, UK
    Microbiology 150:3631-45. 2004
    ..Our data provide valuable new information for the development of intervention strategies...
  29. ncbi Variations of Porphyromonas gingivalis fimbriae in relation to microbial pathogenesis
    Atsuo Amano
    Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, Suita Osaka, Japan
    J Periodontal Res 39:136-42. 2004
    ..In this short review, variations of fimbriae in relation to microbial pathogenesis are discussed...
  30. pmc DNA adenine methylation regulates virulence gene expression in Salmonella enterica serovar Typhimurium
    Roberto Balbontin
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, 41080 Seville, Spain
    J Bacteriol 188:8160-8. 2006
    ..The requirement for DNA adenine methylation in the regulation of specific virulence genes suggests that certain defects of Salmonella Dam(-) mutants in the mouse model may be caused by altered patterns of gene expression...
  31. pmc Evidence that focal adhesion complexes power bacterial gliding motility
    Tâm Mignot
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Science 315:853-6. 2007
    ..The Frz chemosensory system coordinated the two motility systems. The dynamics of protein cluster localization suggest that intracellular motors and force transmission by dynamic focal adhesions can power bacterial motility...
  32. pmc Housekeeping sortase facilitates the cell wall anchoring of pilus polymers in Corynebacterium diphtheriae
    Anu Swaminathan
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Mol Microbiol 66:961-74. 2007
    ....
  33. ncbi Characterization of the pilF-pilD pilus-assembly locus of Neisseria gonorrhoeae
    N E Freitag
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620, USA
    Mol Microbiol 16:575-86. 1995
    ..Although expression of orfX and pilD appears to be transcriptionally coupled, mutants containing transposon insertions in orfX expressed pili. Unlike either pilF or pilD mutants, orfX mutants were also competent for DNA transformation...
  34. pmc The Salmonella enterica serotype Typhimurium lpf, bcf, stb, stc, std, and sth fimbrial operons are required for intestinal persistence in mice
    Eric H Weening
    Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, One Shields Ave, Davis, CA 95616 8645, USA
    Infect Immun 73:3358-66. 2005
    ..Collectively, these data show that six fimbrial operons (lpf, bcf, stb, stc, std, and sth) contribute to long-term intestinal carriage of S. enterica serotype Typhimurium in genetically resistant mice...
  35. pmc Regulation of type 1 fimbriae by unlinked FimB- and FimE-like recombinases in uropathogenic Escherichia coli strain CFT073
    Andrew Bryan
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA
    Infect Immun 74:1072-83. 2006
    ..Together these results indicate that ipuA has fimB-like on-to-off and off-to-on fim switching activity and that ipbA has the ability to switch fim from the off to the on orientation...
  36. pmc Physical properties of Escherichia coli P pili measured by optical tweezers
    Jana Jass
    Department of Microbiology and Immunology, The Lawson Health Research Institute, University of Western Ontario, London, Ontario, N6A 4V2, Canada
    Biophys J 87:4271-83. 2004
    ..This unfolding can elongate the pilus up to 7 +/- 2 times. In region III, pili elongate in a nonlinear manner as a result of stretching until the bond ruptures...
  37. pmc Utilization of an intracellular bacterial community pathway in Klebsiella pneumoniae urinary tract infection and the effects of FimK on type 1 pilus expression
    David A Rosen
    Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
    Infect Immun 76:3337-45. 2008
    ..Thus, K. pneumoniae appears programmed for minimal expression of type 1 pili, which may explain, in part, why K. pneumoniae is a less prevalent etiologic agent of UTI than UPEC...
  38. ncbi Klebsiella pneumoniae type 3 pili facilitate adherence and biofilm formation on abiotic surfaces
    Patrick Di Martino
    Laboratoire ERRMECe équipe Microbiologie, U F R Sciences et Techniques, 95302 Cergy Pontoise, France
    Res Microbiol 154:9-16. 2003
    ..Thus, type 3 pili constitute the main K. pneumoniae adhesive factor, facilitating adherence and biofilm formation on abiotic surfaces of strains of different origins...
  39. ncbi Use of Lactococcus lactis expressing pili from group B Streptococcus as a broad-coverage vaccine against streptococcal disease
    Scilla Buccato
    Novartis Vaccines, 1 53100 Siena, Italy
    J Infect Dis 194:331-40. 2006
    ..These data pave the way to the design of pilus-based, multivalent live vaccines against streptococcal pathogens...
  40. ncbi Pilus-mediated adhesion of Neisseria meningitidis: the essential role of cell contact-dependent transcriptional upregulation of the PilC1 protein
    M K Taha
    unité des Neisseria, Institut Pasteur, Paris, France
    Mol Microbiol 28:1153-63. 1998
    ..Taken together, these data demonstrate that cell contact-dependent upregulation of the transcription of pilC1 at PC1.3 is essential for meningococcal pilus-mediated adhesion...
  41. pmc Bundle-forming pili and EspA are involved in biofilm formation by enteropathogenic Escherichia coli
    Cristiano G Moreira
    Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
    J Bacteriol 188:3952-61. 2006
    ..Taken together, these studies allowed us to propose a model of EPEC biofilm formation...
  42. ncbi Reconstitution of pilus assembly reveals a bacterial outer membrane catalyst
    Mireille Nishiyama
    Institute of Molecular Biology and Biophysics, Eidgenossische Technische Hochschule ETH Zurich, 8093 Zurich, Switzerland
    Science 320:376-9. 2008
    ..Our results contribute to a mechanistic understanding of protein-catalyzed biogenesis of supramolecular protein complexes at the bacterial outer cell membrane...
  43. pmc Neisseria gonorrhoeae PilV, a type IV pilus-associated protein essential to human epithelial cell adherence
    H C Winther-Larsen
    Biotechnology Center of Oslo and Department of Microbiology, Institute of Pharmacy, University of Oslo, 0316 Oslo, Norway
    Proc Natl Acad Sci U S A 98:15276-81. 2001
    ..Purified Tfp from pilV mutants contain PilC adhesin at reduced levels. Taken together, these data support a model in which PilV functions in adherence by promoting the functional display of PilC in the context of the pilus fiber...
  44. ncbi The distinct binding specificities exhibited by enterobacterial type 1 fimbriae are determined by their fimbrial shafts
    Matthew J Duncan
    Molecular Genetics and Microbiology and Pathology, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 280:37707-16. 2005
    ..The capacity of type 1 fimbrial shafts to modulate the tissue tropism of different enterobacterial species represents a novel function for these highly organized structures...
  45. pmc Group A Streptococcus produce pilus-like structures containing protective antigens and Lancefield T antigens
    Marirosa Mora
    Chiron Vaccines, Via Fiorentina 1, 53100 Siena, Italy
    Proc Natl Acad Sci U S A 102:15641-6. 2005
    ..The data indicate that induction of a protective immune response against these structures may be a useful strategy for development of a vaccine against disease caused by GAS infection...
  46. ncbi Type II protein secretion and its relationship to bacterial type IV pili and archaeal flagella
    Christopher R Peabody
    Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093 0116, USA
    Microbiology 149:3051-72. 2003
    ..The archaeal TM proteins are twice as large as the bacterial TM proteins, suggesting an oligomeric structure for the latter...
  47. pmc A novel EspA-associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells
    S Knutton
    Institute of Child Health, University of Birmingham, Birmingham B16 8ET, UK
    EMBO J 17:2166-76. 1998
    ....
  48. pmc Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli
    Danelle S Eto
    Division of Cell Biology and Immunology, Pathology Department, University of Utah, Salt Lake City, Utah, United States of America
    PLoS Pathog 3:e100. 2007
    ..Cumulatively, these results indicate that beta1 and alpha3 integrins are functionally important receptors for type 1 pili-expressing bacteria within the urinary tract and possibly at other sites within the host...
  49. ncbi Identification and characterization of pilG, a highly conserved pilus-assembly gene in pathogenic Neisseria
    T Tønjum
    Kaptein W Wilhelmsen og Frues Bakteriologiske Institutt, Rikshospitalet National Hospital, University of Oslo, Norway
    Mol Microbiol 16:451-64. 1995
    ..These findings could not be ascribed to pilin-gene alterations or to polarity exerted on pilD expression. The results indicated that PilG exerts its own independent role in neisserial pilus biogenesis...
  50. ncbi Shear-dependent 'stick-and-roll' adhesion of type 1 fimbriated Escherichia coli
    Wendy E Thomas
    Department of Bioengineering, University of Washington, Seattle, WA, 98195, USA
    Mol Microbiol 53:1545-57. 2004
    ..This 'stick-and-roll' adhesion could allow type 1 fimbriated bacteria to move along mannosylated surfaces under relatively low flow conditions and to accumulate preferentially in high shear regions...
  51. ncbi Type 1 fimbriae and extracellular polysaccharides are preeminent uropathogenic Escherichia coli virulence determinants in the murine urinary tract
    Farah K Bahrani-Mougeot
    Division of Infectious Diseases, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Microbiol 45:1079-93. 2002
    ..These results confirm the pre-eminence of type 1 fimbriae, establish the importance of extracellular polysaccharides in the pathogenesis of UTI and identify new urovirulence determinants...
  52. ncbi Synergistic biofilm formation by Treponema denticola and Porphyromonas gingivalis
    Mitsunori Yamada
    Department of Oral Biology, State University of New York, Buffalo, NY 14214, USA
    FEMS Microbiol Lett 250:271-7. 2005
    ..gingivalis. The major fimbriae and Arg-gingipain B of P. gingivalis also play important roles in biofilm formation with T. denticola...
  53. pmc Evolutionary and functional relationships of colonization factor antigen i and other class 5 adhesive fimbriae of enterotoxigenic Escherichia coli
    Ravi P Anantha
    Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910 7500, USA
    Infect Immun 72:7190-201. 2004
    ..These results were corroborated with similar results from the Caco-2 cell adherence assay. Our findings suggest that the minor subunits of class 5 fimbriae may be superior to whole fimbriae in inducing antiadhesive immunity...
  54. ncbi Multiple insertions of fimbrial operons correlate with the evolution of Salmonella serovars responsible for human disease
    A Folkesson
    Mikrobiologiskt och Tumörbiologiskt Centrum, Karolinska Institutet, Box 280, Tomtebodavagen 34, S 17177 Stockholm, Sweden
    Mol Microbiol 33:612-22. 1999
    ..coli. It is suggested that the multiple insertions of fimbrial genes that have occurred in the cs7 region have contributed to phylogenetic diversity and host adaptation of Salmonella spp...
  55. ncbi FGL chaperone-assembled fimbrial polyadhesins: anti-immune armament of Gram-negative bacterial pathogens
    Anton Zavialov
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden
    FEMS Microbiol Rev 31:478-514. 2007
    ..The FGL chaperone-assembled fimbrial polyadhesins are attractive targets for vaccine and drug design...
  56. pmc Multiple genes repress motility in uropathogenic Escherichia coli constitutively expressing type 1 fimbriae
    Amy N Simms
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Bacteriol 190:3747-56. 2008
    ..Our results indicate that repression of motility in CFT073 fim L-ON is not solely due to the constitutive expression of type 1 fimbriae on the surfaces of the bacteria and that multiple genes may contribute to this repression...
  57. pmc Snapshots of usher-mediated protein secretion and ordered pilus assembly
    E T Saulino
    Department of Molecular Microbiology and Microbial Pathogenesis, Washington University School of Medicine, St Louis, MO 63110 1010, USA
    Proc Natl Acad Sci U S A 97:9240-5. 2000
    ..Specialized pilin subunits play unique roles both in this multimerization and in the final ultrastructure of the adhesive pilus...
  58. ncbi Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype
    Takahiro Kato
    Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, Suita Osaka, Japan
    Cell Microbiol 9:753-65. 2007
    ..In a mouse abscess model, the type II clones significantly induced serum IL-1beta and IL-6, as well as other infectious symptoms. These results suggest that type II fimbriae are a critical determinant of P. gingivalis virulence...
  59. pmc Covert operations of uropathogenic Escherichia coli within the urinary tract
    Jean M Bower
    Pathology Department, Division of Cell Biology and Immunology, University of Utah School of Medicine, 30 North 1900 East, Salt Lake City, Utah 84132 2501, USA
    Traffic 6:18-31. 2005
    ..Here we review the diverse mechanisms and consequences of host cell invasion by UPEC, focusing also on the impact of these processes on the persistence and recurrence of UTIs...
  60. pmc The affinity of the FimH fimbrial adhesin is receptor-driven and quasi-independent of Escherichia coli pathotypes
    Julie Bouckaert
    Department of Ultrastructure, Vrije Universiteit Brussel, Flanders Interuniversity Institute for Biotechnology VIB, Pleinlaan 2, 1050 Brussels, Belgium
    Mol Microbiol 61:1556-68. 2006
    ..Our results imply that the carbohydrate expression profile of targeted host tissues and of natural inhibitors in urine, such as Tamm-Horsfall protein, are stronger determinants of adhesion than FimH variation...
  61. pmc Structure and assembly of P-pili: a protruding hinge region used for assembly of a bacterial adhesion filament
    Xiang Qi Mu
    Department of Physiology and Biophysics, Boston University School of Medicine, 715 Albany Street W302, Boston, MA 02118, USA
    Proc Natl Acad Sci U S A 103:9861-6. 2006
    ..This model of the P-pilus filament provides an insight into the mechanism of assembly of a macromolecular complex essential for initiation of kidney infection by these bacteria...
  62. ncbi Structural basis of tropism of Escherichia coli to the bladder during urinary tract infection
    Chia Suei Hung
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Microbiol 44:903-15. 2002
    ..coli (EHEC) possess a sequence variation within the mannose-binding pocket of FimH, suggesting a naturally occurring mechanism of attenuation in EHEC bacteria that would prevent them from being targeted to the urinary tract...
  63. ncbi Dancing with the host; flow-dependent bacterial adhesion
    Ralph R Isberg
    Howard Hughes Medical Institute, Boston, MA 02111, USA
    Cell 110:1-4. 2002
    ..Some pili promote efficient adhesion only when they are subjected to shear stress, as found during the flow of blood over endothelium or mucous over the surface of the epithelium...
  64. ncbi Bacterial fimbriae activate human peripheral blood monocytes utilizing TLR2, CD14 and CD11a/CD18 as cellular receptors
    Tomohiko Ogawa
    Department of Oral Microbiology, Asahi University School of Dentistry, Gifu, Japan
    Eur J Immunol 32:2543-50. 2002
    b>Bacterial fimbriae are associated with a specific adherence factor, adhesin, in their microbial etiology...
  65. ncbi A bacteriophage encoding a pathogenicity island, a type-IV pilus and a phage receptor in cholera bacteria
    D K Karaolis
    Center for Vaccine Development, Division of Hospital Epidemiology, University of Maryland School of Medicine, Baltimore 21201, USA
    Nature 399:375-9. 1999
    ..Our results are the first description of a phage that encodes a receptor for another phage and of a virus-virus interaction that is necessary for bacterial pathogenicity...
  66. ncbi Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants
    Mikkel Klausen
    Molecular Microbial Ecology Group, BioCentrum DTU, Building 301, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Mol Microbiol 48:1511-24. 2003
    ..Bacterial migration prevented the formation of larger microcolonial structures in the wild-type biofilms. The results are discussed in relation to the current model for P. aeruginosa biofilm development...
  67. pmc The molecular switch that activates the cell wall anchoring step of pilus assembly in gram-positive bacteria
    Anjali Mandlik
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
    Proc Natl Acad Sci U S A 105:14147-52. 2008
    ..Cell wall anchoring ensues when the pilus polymer assembled on the pilus-specific sortase is transferred to the minor pilin presented by the housekeeping sortase via lysine-mediated transpeptidation...
  68. ncbi Differential binding to and biofilm formation on, HEp-2 cells by Salmonella enterica serovar Typhimurium is dependent upon allelic variation in the fimH gene of the fim gene cluster
    Jennifer D Boddicker
    Department of Microbiology, College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Mol Microbiol 45:1255-65. 2002
    ..Indeed, the increased efficiency of biofilm formation in the murine intestinal tract confirmed the role of one of the fimH alleles in this process...
  69. pmc Assembly of fimbrial structures in Pseudomonas aeruginosa: functionality and specificity of chaperone-usher machineries
    Ségolène Ruer
    Laboratoire d Ingenierie des Systemes Macromoleculaires, CNRS IBSM UPR9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 189:3547-55. 2007
    ..Finally, we observed that the CupB and CupC systems are specialized in the assembly of their own fimbrial subunits and are not exchangeable...
  70. pmc Chaperone-subunit-usher interactions required for donor strand exchange during bacterial pilus assembly
    Michelle M Barnhart
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110 1093, USA
    J Bacteriol 185:2723-30. 2003
    ..Furthermore, the donor strand exchange assembly mechanism between subunits was found to be necessary for subunit translocation across the outer membrane usher...
  71. pmc The presence of the pilus locus is a clonal property among pneumococcal invasive isolates
    Sandra I Aguiar
    Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina Universidade de Lisboa, Lisboa, Portugal
    BMC Microbiol 8:41. 2008
    ....
  72. pmc Twitching motility contributes to the role of pili in corneal infection caused by Pseudomonas aeruginosa
    Irandokht Zolfaghar
    School of Optometry, University of California, Berkeley, California 94720, USA
    Infect Immun 71:5389-93. 2003
    ..02). Thus, twitching motility contributed to the role of pili in corneal disease but was not involved in the role of pili in adherence to or invasion of corneal epithelial cells...
  73. ncbi Type 1 pili-mediated adherence of Escherichia coli strain LF82 isolated from Crohn's disease is involved in bacterial invasion of intestinal epithelial cells
    J Boudeau
    Pathogenie Bacterienne Intestinale, Laboratoire de Bacteriologie, Faculte de Pharmacie, Universite d Auvergne, 28, Place Henri Dunant, 63001 Clermont Ferrand, France
    Mol Microbiol 39:1272-84. 2001
    ....
  74. pmc A second pilus type in Streptococcus pneumoniae is prevalent in emerging serotypes and mediates adhesion to host cells
    Fabio Bagnoli
    Department of Cellular Microbiology and Bioinformatics, Novartis Vaccines, University of Siena, Siena 53100, Italy
    J Bacteriol 190:5480-92. 2008
    ..Thus, pneumococci encode at least two types of pili that play a role in the initial host cell contact to the respiratory tract and are potential antigens for inclusion in a new generation of pneumococcal vaccines...
  75. pmc Sortase-mediated assembly and surface topology of adhesive pneumococcal pili
    Stefan Fälker
    Department of Bacteriology, Swedish Institute for Infectious Disease Control, SE 171 82 Solna, Sweden
    Mol Microbiol 70:595-607. 2008
    ..In contrast, SrtD does not act as a pilus subunit polymerase, but instead is required for wild-type focal presentation of the pilus at the cell surface...
  76. ncbi Streptococcus pyogenes pili promote pharyngeal cell adhesion and biofilm formation
    Andrea G O Manetti
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100, Siena, Italy
    Mol Microbiol 64:968-83. 2007
    ..These data support the role of pili in GAS adherence and colonization and suggest a general role of pili in all pathogenic streptococci...
  77. ncbi Chaperone-assisted self-assembly of pili independent of cellular energy
    F Jacob-Dubuisson
    Department of Molecular Microbiology, Washington University Medical School, St Louis, Missouri 63110 1093
    J Biol Chem 269:12447-55. 1994
    ..We suggest that the secretion of pili across the outer membrane may be independent of cellular energy and thermodynamically driven...
  78. ncbi Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli
    Caroline Blumer
    Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg Universitat Mainz, Becherweg 15, 55099 Mainz, Germany
    Microbiology 151:3287-98. 2005
    ..Together, the data show a role for LrhA as a repressor of type 1 fimbrial expression, and thus as a regulator of the initial stages of biofilm development and, presumably, bacterial adherence to epithelial host cells also...
  79. pmc The complete general secretory pathway in gram-negative bacteria
    A P Pugsley
    Unité de Génétique Moléculaire, Institut Pasteur, Paris, France
    Microbiol Rev 57:50-108. 1993
    ..This recently discovered branch pathway comprises at least 14 gene products. Other, simpler terminal branches of the GSP are also used by gram-negative bacteria to secrete a more limited range of extracellular proteins...
  80. ncbi Structural analysis of the Saf pilus by electron microscopy and image processing
    Osman Salih
    Institute of Structural Molecular Biology at UCL Birkbeck, London WC1E 7HX, UK
    J Mol Biol 379:174-87. 2008
    ..Short intersubunit linker regions provide the molecular basis for flexibility of the Saf pilus by acting as molecular hinges allowing a large range of movement between consecutive subunits in the fibre...
  81. ncbi Fiber assembly by the chaperone-usher pathway
    Frederic G Sauer
    Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Ave, New Haven, CT 06536, USA
    Biochim Biophys Acta 1694:259-67. 2004
    ..Chaperone dissociation releases the folding energy that drives subunit incorporation into the fiber, which grows through a pore formed by the outer-membrane usher...
  82. pmc High-force generation is a conserved property of type IV pilus systems
    Martin Clausen
    Department for Biology, Westfalische Wilhelms Universitat, Munster, Germany
    J Bacteriol 191:4633-8. 2009
    ..Thus, M. xanthus harbors at least two different retraction motors. Our results demonstrate that the major physical properties are conserved between bacteria that are phylogenetically distant and pursue very different lifestyles...
  83. pmc P. gingivalis accelerates gingival epithelial cell progression through the cell cycle
    Masae Kuboniwa
    Department of Preventive Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan
    Microbes Infect 10:122-8. 2008
    ..The ability of P. gingivalis, a common inhabitant of the subgingival crevice, to accelerate cell cycling could have biological consequences for barrier and signaling functions, and for physiological status, of the gingival epithelium...
  84. pmc Catch-bond model derived from allostery explains force-activated bacterial adhesion
    Wendy Thomas
    Department of Bioengineering, University of Washington, Seattle, Washington, USA
    Biophys J 90:753-64. 2006
    ..This strongly suggests that FimH forms allosteric catch bonds. The model advances our understanding of both catch bonds and the role of allostery in regulating protein activity...
  85. ncbi Combining sites of bacterial fimbriae
    Henri De Greve
    Department of Molecular and Cellular Interactions, VIB, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    Curr Opin Struct Biol 17:506-12. 2007
    ..Finally, glycan recognition by fimbrial adhesins has often been found to coincide with the binding of cell-surface integrins and components of the extracellular matrix, such as collagen IV and laminin...
  86. pmc Uropathogenic Escherichia coli potentiates type 1 pilus-induced apoptosis by suppressing NF-kappaB
    D J Klumpp
    Department of Urology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Infect Immun 69:6689-95. 2001
    ..These results suggest that UPEC blocks NF-kappaB and thereby enhances type 1 pili-induced apoptosis as a component of the uropathogenic program...
  87. ncbi Genetic and functional evidence that Type IV pili are required for social gliding motility in Myxococcus xanthus
    S S Wu
    Department of Biochemistry, Stanford University School of Medicine, CA 94305, USA
    Mol Microbiol 18:547-58. 1995
    ..These results indicate that the pili of M. xanthus belong to the Type IV family of pili, and demonstrate that these pili are actually required for social motility...
  88. ncbi (p)ppGpp regulates type 1 fimbriation of Escherichia coli by modulating the expression of the site-specific recombinase FimB
    Anna Aberg
    Department of Molecular Biology, Umea University, S 90187 Umea, Sweden
    Mol Microbiol 60:1520-33. 2006
    ..Our findings suggest that the (p)ppGpp-stimulated expression of type 1 fimbriae may be relevant during the interaction of pathogenic E. coli with the host...
  89. pmc A pneumococcal pilus influences virulence and host inflammatory responses
    M A Barocchi
    Chiron Vaccines, 53100 Siena, Italy
    Proc Natl Acad Sci U S A 103:2857-62. 2006
    ....
  90. pmc Roles of the sortases of Streptococcus pneumoniae in assembly of the RlrA pilus
    Julianna LeMieux
    Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    J Bacteriol 190:6002-13. 2008
    ....
  91. pmc CD46-independent binding of neisserial type IV pili and the major pilus adhesin, PilC, to human epithelial cells
    Marieluise Kirchner
    Max Planck Institute for Infection Biology Department of Molecular Biology, Schumannstrasse 21 22, 10117 Berlin, Germany
    Infect Immun 73:3072-82. 2005
    ..These data support the notion that pilus-mediated gonococcal infection of epithelial cells can occur in a CD46-independent manner, thus questioning the function of CD46 as an essential pilus receptor for pathogenic neisseriae...
  92. ncbi Impact of biofilm matrix components on interaction of commensal Escherichia coli with the gastrointestinal cell line HT-29
    X Wang
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Box 280, 17177, Stockholm, Sweden
    Cell Mol Life Sci 63:2352-63. 2006
    ..In addition, a high immunostimulatory flagellin was identified. Thus, the consequences of expression of extracellular matrix components on commensal bacterial-host interactions are complex...
  93. pmc The 'P-usher', a novel protein transporter involved in fimbrial assembly and TpsA secretion
    Ségolène Ruer
    UPR9027 CNRS, Systémes membranaires et pathogénicité chez Pseudomonas aeruginosa, LISM, IBSM, Marseille, France
    EMBO J 27:2669-80. 2008
    ..The P-usher defines a novel transport pathway involving a molecular machine made with old spare parts...
  94. pmc Meningococcal type IV pili recruit the polarity complex to cross the brain endothelium
    Mathieu Coureuil
    Universite Paris Descartes, Faculte de Medecine, Inserm U 570, 75015 Paris, France
    Science 325:83-7. 2009
    ....
  95. ncbi Assembly of pili on the surface of Bacillus cereus vegetative cells
    Jonathan M Budzik
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 66:495-510. 2007
    ..B. anthracis sortase A (srtA), but not sortase B (srtB) or C (srtC), is required for proper anchoring of pili to the bacterial envelope, suggesting that BcpA/BcpB pili are linked to peptidoglycan cross-bridges...
  96. pmc 3D structure/function analysis of PilX reveals how minor pilins can modulate the virulence properties of type IV pili
    Sophie Helaine
    Institut National de la Sante et de la Recherche Medicale, U570, 75015 Paris, France
    Proc Natl Acad Sci U S A 104:15888-93. 2007
    ....
  97. ncbi Type 1 fimbriae deliver an LPS- and TLR4-dependent activation signal to CD14-negative cells
    M Hedlund
    Department of Laboratory Medicine, Division of Microbiology, University of Lund, Solvegatan 23, S 223 62 Lund, Sweden
    Mol Microbiol 39:542-52. 2001
    ..The results illustrate how the host "sees" LPS and other microbial products not as purified molecules but as complexes, and that fimbriae determine the molecular context in which LPS is presented to host cells...
  98. ncbi Helicobacter exploits integrin for type IV secretion and kinase activation
    Terry Kwok
    Department of Medical Microbiology, Otto von Guericke University, Leipziger Strasse 44, D 39120 Magdeburg, Germany
    Nature 449:862-6. 2007
    ..Our findings provide insights into the role of integrins in H.-pylori-induced pathogenesis. CagL may be exploited as a new molecular tool for our further understanding of integrin signalling...
  99. ncbi The type-4 pilus is the major virulence-associated adhesin of Pseudomonas aeruginosa--a review
    H P Hahn
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    Gene 192:99-108. 1997
    ..In addition to the major pilus adhesin, exoenzyme S and, as recent studies indicate, flagella, are further protein adhesins of Pa with GSL receptor binding specificities similar to those of pili...
  100. ncbi Crystal structure of chaperone protein PapD reveals an immunoglobulin fold
    A Holmgren
    Department of Molecular Biology, Biomedical Centre, Uppsala, Sweden
    Nature 342:248-51. 1989
    ..The chaperone protein PapD mediates assembly of pili in Escherichia coli. Its polypeptide chain folds into two immunoglobulin-type domains that are homologous in sequence to the human lymphocyte differentiation antigen Leu-1/CD5...
  101. pmc Porphyromonas gingivalis fimbria-dependent activation of inflammatory genes in human aortic endothelial cells
    Hsin Hua Chou
    School of Dentistry, Taipei Medical University, Taipei, Taiwan
    Infect Immun 73:5367-78. 2005
    ..gingivalis fimbria-mediated invasion upregulates inflammatory gene expression in HAEC and in aortic tissue and indicates that invasive P. gingivalis infection accelerates inflammatory responses directly in the aorta...

Research Grants65

  1. ROLE OF TNF IN BLADDER INFLAMMATION
    David J Klumpp; Fiscal Year: 2010
    ..This project will determine the prevalence of RANTES as a novel IC biomarker in urines and then quantify the efficacy of mechanistically-directed therapies in murine models. ..
  2. Biacore T100
    John Carson; Fiscal Year: 2006
    ..This quantitative information will enable us to understand how molecular interaction networks mediate specific cell biological processes and how such networks are disrupted in disease [unreadable] [unreadable]..
  3. EFFICACY OF E COLI PILUS VACCINE FOR URINARY INFECTION
    Joan Fusco; Fiscal Year: 1990
    ..These experiments should provide the first supportive evidence that a multiple-pilus vaccine can be efficacious for UTI before extensive human clinical testing begins."..
  4. Bacterial interactions in biofilms
    Richard Hull; Fiscal Year: 2004
    ..Results of the proposed experiments may illuminate new targets for intervention therapies that disrupt biofilm formation. This may lead to more effective treatment of catheter-associated infections. ..
  5. Development of an additive model to study the significance of heat-labile and hea
    Weiping Zhang; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  6. Genetica Analysis of S. Pyogenes Virulence Factors
    JUNE SCOTT; Fiscal Year: 2008
    ..This will suggest new targets for development of therapeutic interventions to prevent and/or ameliorate the many types of GAS diseases. ..
  7. Salmonella Invasion Gene Regulation
    Bradley Jones; Fiscal Year: 2004
    ..abstract_text> ..
  8. Pathogenesis of EAST1 toxin in ETEC associated diarrhea disease
    Weiping Zhang; Fiscal Year: 2008
    ..The determination of virulence significance of EAST1 toxin in diarrheal disease will provide essential information to prevent or control this disease. [unreadable] [unreadable] [unreadable] [unreadable]..
  9. Proline uptake in staphylococcus aureus pathogenesis
    WILLIAM SCHWAN; Fiscal Year: 2002
    ..The results of this study will help us understand the role of proline transport in S. aureus infections. ..
  10. Novel Mechanism of Uranium Reduction Via Microbial Nanowires
    Gemma Reguera; Fiscal Year: 2011
    ....
  11. OmpR and acid regulation of Escherichia coli fim genes
    WILLIAM SCHWAN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  12. Mucosal immune response to oral cholera vaccine Peru 15
    Firdausi Qadri; Fiscal Year: 2007
    ..cholerae. ..
  13. INTERCELLULAR SIGNALING IN MYXOCOCCUS XANTHUS
    Wenyuan Shi; Fiscal Year: 2010
    ....
  14. Chemical genetic approach to Vibrio cholerae pathogensis
    DEBORAH HUNG; Fiscal Year: 2007
    ..John Mekalanos, thus allowing her to become a productive, independent physician-scientist. ..
  15. Glycosylation & Biogenesis of Streptococcal Fimbriae
    Hui Wu; Fiscal Year: 2007
    ..Therefore, elucidation of Fap1 glycosylation may provide a framework for understanding the role of glycoslyation in bacterial pathogenesis. ..
  16. Functional Analysis of a Streptococcal GPIb Adhesin
    Hui Wu; Fiscal Year: 2007
    ..This information may lead to new approaches for prevention and treatment of infective endocarditis and other cardiovascular diseases. [unreadable] [unreadable]..
  17. Role of OxyR in P. aeruginosa Biofilm Resistance to H202
    DANIEL HASSETT; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  18. pilE mRNA Analysis in Gonococci
    Stuart Hill; Fiscal Year: 2007
    ..These studies should provide unique opportunities for the development of novel pharmaceuticals for therapeutic intervention into gonorrheal or meningococcal disease. [unreadable] [unreadable] [unreadable] [unreadable]..
  19. Intergeneric Signaling Molecules of S. cristatus
    Hua Xie; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  20. Genetic Analysis-Adhesion of S Sanguis to S Pellicle
    Hui Wu; Fiscal Year: 2008
    ..abstract_text> ..
  21. Mucosal Lymphocyte Homing in the Head and Neck
    David Pascual; Fiscal Year: 2008
    ..n. challenge with CT. From this work, we will learn how routes of immunizations can be used to effectively target vaccines to nonintestinal mucosal sites. ..
  22. Type III Secretion in Bordetella
    JEFFERY MILLER; Fiscal Year: 2008
    ..3. Investigate the effects of type III secretion and other BtrS-regulated phenotypes during respiratory tract infection by B. pertussis and B. bronchiseptica. ..
  23. H. hepaticus: the pathogenesis of IBD
    David Schauer; Fiscal Year: 2007
    ..It is hoped that these studies will lead to the development of new strategies for the treatment and the prevention of IBD. ..
  24. The eae Gene Cluster of Enteropathogenic E.coli
    Michael Donnenberg; Fiscal Year: 2007
    ..The significance of this work is that it will lead to a better understanding of how bacteria inject proteins into host cells and interfere with pathways that protect the cells from death and maintain barrier function. ..
  25. Mucosal Therapy for Autoimmunity
    David Pascual; Fiscal Year: 2008
    ..Studies in Specific Aim 3 will determine the responsible dendritic cell subset that supports regulatory T cell development. ..
  26. Arabidopsis basal innate immunity to bacterial infection
    Sheng Yang He; Fiscal Year: 2010
    ..Food poisoning caused by human pathogen contamination is a major public health concern. ..
  27. 2006 Periodontal Diseases Gordon Research Conference
    Michael Curtis; Fiscal Year: 2006
    ..Funding is requested in order to support the travel costs and meeting expenses of speakers and to provide meeting grants for junior research workers at the beginning of their research careers. [unreadable] [unreadable] [unreadable]..
  28. Molecular Basis for Pseudomonas Recognition of Epithelia
    BARBARA KAZMIERCZAK; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  29. RECOMBINANT LISTERIA VACCINES FOR MELANOMA
    JEFFERY MILLER; Fiscal Year: 2003
    ..monocytogenes. L. innocua is a non-pathogenic member of the Listeria genus. By transferring genes from L. monocytogenes, we will attempt to construct chimeric strains that are immunogenic yet avirulent. ..
  30. B.pseudomallei bioterrorism and quorum sensing
    DANIEL HASSETT; Fiscal Year: 2003
    ..These studies will provide important information that will lead to countermeasures against a potential bioterrorist attack using the potentially lethal gram-negative bacterium, B. pseudomallei. ..
  31. Type IV Pilins as Vaccines against Bioterrorism Threats
    Michael Donnenberg; Fiscal Year: 2004
    ....
  32. TARGETED DNA DELIVERY FOR SALIVARY IGA RESPONSES
    David Pascual; Fiscal Year: 2004
    ..These studies will provide the basis for future development of subunit vaccines to target the NALT for eliciting protective immunity in the oral cavity and SALT. ..
  33. INTERLEUKIN-12 REGULATION BY DR-FIMBRIATED E. COLI
    Pawel Goluszko; Fiscal Year: 2002
    ..The investigators propose to evaluate in three Specific Aims the general hypothesis that cross-linking of DAF on human monocytes/macrophages by E. coli bearing Dr fimbriae inhibits IL-2 production. ..
  34. Salmonella Gene Expression in Complex Environments
    Michael McClelland; Fiscal Year: 2004
    ..As a class, these methods should be applicable to studying gene expression in any bacterial or eukaryotic microbial community. ..
  35. BORDETELLA VIRULENCE REGULON IN VITRO AND IN VIVO
    JEFFERY MILLER; Fiscal Year: 2005
    ..Differences in expression profiles between B. pertussis, B. parapertussis and B. bronchiseptica will also be determined in an effort to understand subspecies-specific differences in Bordetella-host interactions. ..
  36. MUCOSAL TRANSGENE VACCINATION FOR HIV
    David Pascual; Fiscal Year: 2003
    ..These studies will provide the basis for future development of subunit vaccines to target the NALT for eliciting protective immunity via the common mucosal immune system. ..
  37. Chronic Prostatitis Collaborative Research Network
    Anthony Schaeffer; Fiscal Year: 2007
    ..abstract_text> ..
  38. IRON, FUR AND ALGINATE PRODUCTION IN P AERUGINOSA
    DANIEL HASSETT; Fiscal Year: 2002
    ..Information derived from the studies proposed herein will advance the current knowledge about the role of these factors in the pathogenesis of P. aeruginosa infections in CF. ..
  39. Virulence inhibitors as candidate therapeutics in CF
    Stephen Lory; Fiscal Year: 2005
    ....
  40. Virulence Typing of Pseudomonas Clinical Isolates
    BARBARA KAZMIERCZAK; Fiscal Year: 2006
    ..This can have significant consequences both for public health, by limiting the emergence of multi-drug resistant pathogens in our hospitals, and for containing the cost of medical care. [unreadable] [unreadable]..
  41. Haemophilus ducreyi Resistance to Antimicrobial Peptides
    MARGARET BAUER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  42. MUCOSAL IMMUNITY ELICITED BY SALMONELLA VACCINE DELIVERY
    David Pascual; Fiscal Year: 2009
    ..Thus, from these studies we will learn the specific mechanisms induced by this anti-inflammatory vaccine. ..
  43. Mucosal Vaccines for Plague
    David Pascual; Fiscal Year: 2006
    ..pestis challenge. From these studies, we will produce efficacious vaccines to plague, as well as a supporting vaccine regimen, that will confer protective immunity in the mucosal and systemic immune compartments. ..
  44. YagZ Pilus Adhesin of Attaching and Effacing E. coli
    Jorge A Giron; Fiscal Year: 2010
    ..Specific Aim 1: To define the role of YagZ pili in AEEC adhesion Specific Aim 2. To determine how YagZ pilus regulation occurs in AEEC Specific Aim 3. To determine how YagZ pili biogenesis occurs in AEEC ..
  45. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2010
    ..These detailed structural studies will provide a long-awaited molecular mechanism for the origin licensing stage of the intricate replication initiation process. ..
  46. Diversity-Generating Retroelements in Phage and Bacterial Genomes
    Jeffery F Miller; Fiscal Year: 2010
    ..2. Probe the receptor repertoire and the structural basis of ligand recognition by a DGR-encoded receptor protein. 3. Investigate DGR function in the human gastrointestinal commensal Bacteroides thetaiotaomicron. ..
  47. Inhibitors of c-di-GMP synthesis and degradation
    Stephen Lory; Fiscal Year: 2010
    ..abstract_text> ..
  48. INFLAMMATORY PROCESS PERIODONTAL WOUND HEALING
    Salomon Amar; Fiscal Year: 2002
    ..Aim-2 will focus on gaining insight into the molecular mechanisms which underlie changes in granulation tissue and matrix formation when IL-1 and TNF soluble blockers are injected in periodontal wounds. ..
  49. 0157:H7 INTIMIN AND STX--PATHOGENESIS IN A MOUSE MODEL
    David Schauer; Fiscal Year: 2003
    ..It should also lead to the development of a mouse model for studies of O157:H7 pathogenesis and for testing preventive and therapeutic approaches directed against this important foodborne illness. ..
  50. Novel YcbQ Pili of Enterohemorrhagic E. coli O157:H7
    Jorge Giron; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  51. Signal Trans. and Intestinal Colonization by V.Cholerae
    KENNETH PETERSON; Fiscal Year: 2006
    ..cholerae carrying mutations within acfBC genes; (4) recombinase-based in vitro expression technology (RIVET) will elucidate the role of AcfB in promoting maximal levels of pilus synthesis. ..
  52. Analysis of P. aeruginosa genome diversity and evolution
    Stephen Lory; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  53. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
    ..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
  54. Inhibition of HIV Infection by Oral Bacterial Component
    Hua Xie; Fiscal Year: 2003
    ..Furthermore, the study of P. gingivalis inhibitory protein may lead to a discovery of an additional target to complement the current anti-HIV targets which are either reverse transcriptase or protease. ..
  55. TLR5 Antagonists for Infectious Disease Therapy
    Stephen Lory; Fiscal Year: 2008
    ..The results of this Phase I project will provide in vivo validated TLR5 antagonists for the development of therapeutics to reduce morbidity and mortality in bacterial sepsis and chronic infections. [unreadable] [unreadable] [unreadable]..
  56. Helicobacter hepaticus: pathogenesis of inflammatory bowel disease
    David Schauer; Fiscal Year: 2008
    ..hepaticus. ..
  57. Proteogenome of Anaerobic P. aeruginosa in CF Mucus
    DANIEL HASSETT; Fiscal Year: 2003
    ..Our LONG TERM GOAL is to identify cellular targets that inhibit or significantly compromise the denitrification process that is occurring by P. aeruginosa in the CF airways. ..
  58. E. coli for prevention of Catheter UTI in SCI Patients
    BARBARA TRAUTNER; Fiscal Year: 2006
    ..The candidate plans to combine her clinical background in infectious diseases with the mentored research preformed under this award in order to reduce the secondary infectious complications of mobility disorders. ..
  59. Elucidating Novel Topological Features in Protein Structures
    Todd O Yeates; Fiscal Year: 2010
    ..The proteins to be studied here possess unique features that make them valuable in efforts to achieve a fundamental understanding of protein structure and stability. ..
  60. Treatment and/or Prevention of Urinary Tract Infections
    BARBARA TRAUTNER; Fiscal Year: 2008
    ..An agent to prevent and treat UTI that does not encourage microbial resistance would have a significant impact on public health. [unreadable] [unreadable] [unreadable]..
  61. Systemic Endothelial Consequences of Periodontal Disease
    Salomon Amar; Fiscal Year: 2010
    ..The proposed studies will provide new insights into how periodontal disease contributes to cardiovascular disease risk in human subjects and may lead to new approaches to therapy. ..
  62. New approaches to studying host-chlamydia interactions
    Joanne Engel; Fiscal Year: 2006
    ..trachomatis infection. Together, these studies will significantly advance our knowledge of infectious disease pathogenesis and pave the way for new therapies and vaccines. ..
  63. Clinical Investigation of E.coli Biofilm Formation in the Human Bladder
    BARBARA TRAUTNER; Fiscal Year: 2007
    ..No effective strategy exists to prevent CAUTI in persons who require ongoing catheter use. Identifying genes that are required to cause CAUTI will lead to new strategies to prevent this illness. [unreadable] [unreadable] [unreadable]..
  64. Gonococcal Pilus Structure and Function
    H Seifert; Fiscal Year: 2008
    ..These studies will provide new findings and insights into the structure and functions of the gonococcal pilus. ..