cell membrane

Summary

Summary: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

Top Publications

  1. ncbi Lipid rafts as a membrane-organizing principle
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Science 327:46-50. 2010
  2. ncbi Mechanisms of TGF-beta signaling from cell membrane to the nucleus
    Yigong Shi
    Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544, USA
    Cell 113:685-700. 2003
  3. ncbi Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu
    Stuart J D Neil
    Aaron Diamond AIDS Research Center and Laboratory of Retrovirology, The Rockefeller University, 455 First Avenue, New York, New York 10016, USA
    Nature 451:425-30. 2008
  4. ncbi Imaging intracellular fluorescent proteins at nanometer resolution
    Eric Betzig
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Science 313:1642-5. 2006
  5. pmc Membrane lipids: where they are and how they behave
    Gerrit van Meer
    Bijvoet Center and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Nat Rev Mol Cell Biol 9:112-24. 2008
  6. ncbi Phosphoinositides in cell regulation and membrane dynamics
    Gilbert Di Paolo
    Department of Pathology and Cell Biology, The Taub Institute for Research on Alzheimer s Disease and the Aging Brain, Columbia University Medical Center, New York, New York 10032, USA
    Nature 443:651-7. 2006
  7. ncbi Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?
    Kim A Brogden
    Department of Periodontics and Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, Iowa 52242, USA
    Nat Rev Microbiol 3:238-50. 2005
  8. pmc The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein
    Nanette Van Damme
    The San Diego Department of Veterans Affairs Healthcare System, San Diego, CA 92161, USA
    Cell Host Microbe 3:245-52. 2008
  9. ncbi Calcium signaling
    David E Clapham
    Aldo R Castañeda Professor of Cardiovascular Research, Professor of Neurobiology, Harvard Medical School, Howard Hughes Medical Institute, Enders 1309, Children s Hospital Boston, Boston, MA 02115, USA
    Cell 131:1047-58. 2007
  10. pmc Cells release prions in association with exosomes
    Benoit Fevrier
    Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, Institut Curie, 75005 Paris, France
    Proc Natl Acad Sci U S A 101:9683-8. 2004

Detail Information

Publications343 found, 100 shown here

  1. ncbi Lipid rafts as a membrane-organizing principle
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Science 327:46-50. 2010
    ..Here we review the evidence for how this principle combines the potential for sphingolipid-cholesterol self-assembly with protein specificity to selectively focus membrane bioactivity...
  2. ncbi Mechanisms of TGF-beta signaling from cell membrane to the nucleus
    Yigong Shi
    Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544, USA
    Cell 113:685-700. 2003
    ..This article reviews these latest advances and presents our current understanding on the mechanisms of TGF-beta signaling from cell membrane to the nucleus.
  3. ncbi Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu
    Stuart J D Neil
    Aaron Diamond AIDS Research Center and Laboratory of Retrovirology, The Rockefeller University, 455 First Avenue, New York, New York 10016, USA
    Nature 451:425-30. 2008
    ..Vpu co-localized with CD317 and inhibited these effects. Inhibition of Vpu function and consequent mobilization of tetherin's antiviral activity is a potential therapeutic strategy in HIV/AIDS...
  4. ncbi Imaging intracellular fluorescent proteins at nanometer resolution
    Eric Betzig
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Science 313:1642-5. 2006
    ....
  5. pmc Membrane lipids: where they are and how they behave
    Gerrit van Meer
    Bijvoet Center and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Nat Rev Mol Cell Biol 9:112-24. 2008
    ..enzymes, translocases and transporters, plus the intrinsic physical phase behaviour of lipids and their interactions with membrane proteins, to create the unique compositions and multiple functionalities of their individual membranes?..
  6. ncbi Phosphoinositides in cell regulation and membrane dynamics
    Gilbert Di Paolo
    Department of Pathology and Cell Biology, The Taub Institute for Research on Alzheimer s Disease and the Aging Brain, Columbia University Medical Center, New York, New York 10032, USA
    Nature 443:651-7. 2006
    ..Their functions, besides classical signal transduction at the cell surface, include regulation of membrane traffic, the cytoskeleton, nuclear events and the permeability and transport functions of membranes...
  7. ncbi Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?
    Kim A Brogden
    Department of Periodontics and Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, Iowa 52242, USA
    Nat Rev Microbiol 3:238-50. 2005
    ..In this review the different models of antimicrobial-peptide-induced pore formation and cell killing are presented...
  8. pmc The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein
    Nanette Van Damme
    The San Diego Department of Veterans Affairs Healthcare System, San Diego, CA 92161, USA
    Cell Host Microbe 3:245-52. 2008
    ....
  9. ncbi Calcium signaling
    David E Clapham
    Aldo R Castañeda Professor of Cardiovascular Research, Professor of Neurobiology, Harvard Medical School, Howard Hughes Medical Institute, Enders 1309, Children s Hospital Boston, Boston, MA 02115, USA
    Cell 131:1047-58. 2007
    ..Also discussed is the highly localized nature of Ca(2+)-mediated signal transduction and its specific roles in excitability, exocytosis, motility, apoptosis, and transcription...
  10. pmc Cells release prions in association with exosomes
    Benoit Fevrier
    Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, Institut Curie, 75005 Paris, France
    Proc Natl Acad Sci U S A 101:9683-8. 2004
    ..Furthermore, we found that exosomes bearing PrPsc are infectious. Our data suggest that exosomes may contribute to intercellular membrane exchange and the spread of prions throughout the organism...
  11. ncbi TGF-beta signalling from cell membrane to nucleus through SMAD proteins
    C H Heldin
    Ludwig Institute for Cancer Research, Biomedical Centre, Uppsala, Sweden
    Nature 390:465-71. 1997
    ..proteins has unravelled the mechanisms by which transforming growth factor-beta (TGF-beta) signals from the cell membrane to the nucleus...
  12. ncbi Revitalizing membrane rafts: new tools and insights
    Kai Simons
    Max Planck Institute of Cell Biology and Genetics, Dresden, Germany
    Nat Rev Mol Cell Biol 11:688-99. 2010
    ....
  13. ncbi Cellular motility driven by assembly and disassembly of actin filaments
    Thomas D Pollard
    Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 112:453-65. 2003
    ..Profilin catalyzes the exchange of ADP for ATP, refilling the pool of ATP-actin monomers bound to profilin, ready for elongation...
  14. ncbi Exosomes--vesicular carriers for intercellular communication
    Mikael Simons
    Max Planck Institute for Experimental Medicine, Gottingen, Germany
    Curr Opin Cell Biol 21:575-81. 2009
    ..Only recently light have been shed on the subcellular compartments and mechanisms involved in their biogenesis and secretion opening new avenues to understand their functions...
  15. pmc Molecular basis of bacterial outer membrane permeability revisited
    Hiroshi Nikaido
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
    Microbiol Mol Biol Rev 67:593-656. 2003
    ....
  16. ncbi The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:389-99. 1992
    ..These data imply that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors...
  17. pmc STIM1, an essential and conserved component of store-operated Ca2+ channel function
    Jack Roos
    Torrey Pines Therapeutics, Inc, La Jolla, CA 92037, USA
    J Cell Biol 169:435-45. 2005
    ..We propose that STIM1, a ubiquitously expressed protein that is conserved from Drosophila to mammalian cells, plays an essential role in SOC influx and may be a common component of SOC and CRAC channels...
  18. ncbi Patched1 regulates hedgehog signaling at the primary cilium
    Rajat Rohatgi
    Departments of Developmental Biology, Genetics, and Bioengineering and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 317:372-6. 2007
    ..When Shh bound to Ptc1, Ptc1 left the cilia, leading to accumulation of Smo and activation of signaling. Thus, primary cilia sense Shh and transduce signals that play critical roles in development, carcinogenesis, and stem cell function...
  19. ncbi A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells
    R A Black
    Immunex Corporation, Seattle, Washington 98101, USA
    Nature 385:729-33. 1997
    ..Our results should facilitate the development of therapeutically useful inhibitors of TNF-alpha release, and they indicate that an important function of adamalysins may be to shed cell-surface proteins...
  20. pmc Tetherin inhibits HIV-1 release by directly tethering virions to cells
    David Perez-Caballero
    Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA
    Cell 139:499-511. 2009
    ....
  21. ncbi Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore
    L Song
    Department of Biochemistry, University of Chicago, 920 East 58 Street, Chicago, IL 60637, USA
    Science 274:1859-66. 1996
    ....
  22. ncbi Endocytic recycling
    Frederick R Maxfield
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA
    Nat Rev Mol Cell Biol 5:121-32. 2004
    ..The molecular basis for many of these sorting processes is only partly understood...
  23. ncbi Membrane recognition by phospholipid-binding domains
    Mark A Lemmon
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 809C Stellar Chance Laboratories, 422 Curie Boulevard, Philadelphia, Pennsylvania 19104 6059, USA
    Nat Rev Mol Cell Biol 9:99-111. 2008
    ..A full appreciation of these processes is crucial for understanding how protein localization and membrane topography and trafficking are regulated in cells...
  24. ncbi Functions of cell surface heparan sulfate proteoglycans
    M Bernfield
    Division of Developmental and Newborn Biology, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Biochem 68:729-77. 1999
    ..This chapter focuses on the mechanisms underlying these activities and on the cellular functions that they regulate...
  25. pmc The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia
    Hua Jin
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305 5345, USA
    Cell 141:1208-19. 2010
    ..Thus, we propose that trafficking of BBSome cargoes to cilia entails the coupling of BBSome coat polymerization to the recognition of sorting signals by the BBSome...
  26. ncbi AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding
    Bettina Strack
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 114:689-99. 2003
    ..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
  27. ncbi Seven-transmembrane receptors
    Kristen L Pierce
    The Howard Hughes Medical Institute and the Department of Medicine, Box 3821, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Rev Mol Cell Biol 3:639-50. 2002
    ..Recent findings indicate that the classical models of G-protein coupling and activation of second-messenger-generating enzymes do not fully explain their remarkably diverse biological actions...
  28. pmc Endocytosis and signalling: intertwining molecular networks
    Alexander Sorkin
    Department of Pharmacology, University of Colorado Denver School of Medicine, Aurora, 80010, USA
    Nat Rev Mol Cell Biol 10:609-22. 2009
    ..The mechanistic and functional principles that underlie the relationship between signalling and endocytosis in cell biology are becoming increasingly evident across many systems...
  29. pmc Virulence and immunomodulatory roles of bacterial outer membrane vesicles
    Terri N Ellis
    Box 3711, Biochemistry Department, Duke University Medical Center, Durham, NC 27710, USA
    Microbiol Mol Biol Rev 74:81-94. 2010
    ..Vesicles display antigens and can elicit potent inflammatory and immune responses. In sum, OM vesicles are likely to play a significant role in the virulence of Gram-negative bacterial pathogens...
  30. ncbi Orai1 is an essential pore subunit of the CRAC channel
    Murali Prakriya
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611, USA
    Nature 443:230-3. 2006
    ..These changes in ion selectivity provide strong evidence that Orai1 is a pore subunit of the CRAC channel...
  31. ncbi CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry
    M Vig
    Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Science 312:1220-3. 2006
    ..Although overexpression of CRACM1 did not affect CRAC currents, RNAi-mediated knockdown disrupted its activation. CRACM1 could be the CRAC channel itself, a subunit of it, or a component of the CRAC signaling machinery...
  32. ncbi Membrane curvature and mechanisms of dynamic cell membrane remodelling
    Harvey T McMahon
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 438:590-6. 2005
    ..This is seen during vesicle budding and in the formation of microenvironments. On a larger scale, membrane curvature is a prime player in growth, division and movement...
  33. ncbi An introduction to TRP channels
    I Scott Ramsey
    Howard Hughes Medical Institute, Cardiovascular Department, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Physiol 68:619-47. 2006
    ..The TRP channel gene family and its nomenclature, the encoded proteins and alternatively spliced variants, and the rapidly expanding pharmacology of TRP channels are summarized in online supplemental material...
  34. pmc Lipid A modification systems in gram-negative bacteria
    Christian R H Raetz
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Biochem 76:295-329. 2007
    ..Although not required for growth, the modification enzymes modulate virulence of some gram-negative pathogens. Heterologous expression of lipid A modification enzymes may enable the development of new vaccines...
  35. pmc PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function
    Michelle M Hill
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    Cell 132:113-24. 2008
    ..We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae...
  36. ncbi BAR domains as sensors of membrane curvature: the amphiphysin BAR structure
    Brian J Peter
    Medical Research Council MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 303:495-9. 2004
    ..From this, we predict that BAR domains are in many protein families, including sorting nexins, centaurins, and oligophrenins. The universal and minimal BAR domain is a dimerization, membrane-binding, and curvature-sensing module...
  37. ncbi Protein translocation across the bacterial cytoplasmic membrane
    Arnold J M Driessen
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Haren, The Netherlands
    Annu Rev Biochem 77:643-67. 2008
    ..This review summarizes the present knowledge of the mechanism and structure of the Sec translocase, with a special emphasis on unresolved questions and topics of current research...
  38. ncbi Annexins: linking Ca2+ signalling to membrane dynamics
    Volker Gerke
    Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Munster, Germany
    Nat Rev Mol Cell Biol 6:449-61. 2005
    ..These and related properties enable annexins to participate in several otherwise unrelated events that range from membrane dynamics to cell differentiation and migration...
  39. ncbi SNAP receptors implicated in vesicle targeting and fusion
    T Sollner
    Rockefeller Research Laboratory, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    Nature 362:318-24. 1993
    ....
  40. ncbi Auxin transport inhibitors block PIN1 cycling and vesicle trafficking
    N Geldner
    , , Auf der Morgenstelle 3, , Germany
    Nature 413:425-8. 2001
    ..In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors...
  41. pmc Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits
    Shawn M Ferguson
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    Dev Cell 17:811-22. 2009
    ..Our data also demonstrate that the relationship between dynamin and actin is intimately connected to dynamin's endocytic role and that dynamin terminates a powerful actin- and BAR protein-dependent tubulating activity...
  42. pmc Plasma membrane contributes to the formation of pre-autophagosomal structures
    Brinda Ravikumar
    Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2XY, UK
    Nat Cell Biol 12:747-57. 2010
    ....
  43. pmc SNX27 mediates retromer tubule entry and endosome-to-plasma membrane trafficking of signalling receptors
    Paul Temkin
    Department of Psychiatry, University of California at San Francisco, San Francisco, California 94158, USA
    Nat Cell Biol 13:715-21. 2011
    ..The present results identify a role for retromer in endocytic trafficking of signalling receptors, in regulating a receptor-linked signalling pathway, and in mediating direct endosome-to-plasma membrane traffic...
  44. pmc Cell surface-localized matrix metalloproteinase-9 proteolytically activates TGF-beta and promotes tumor invasion and angiogenesis
    Q Yu
    Molecular Pathology Unit, Massachusetts General Hospital, and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02129 USA
    Genes Dev 14:163-76. 2000
    ..These observations suggest that coordinated CD44, MMP-9, and TGF-beta function may provide a physiological mechanism of tissue remodeling that can be adopted by malignant cells to promote tumor growth and invasion...
  45. pmc The clathrin adaptor AP-1A mediates basolateral polarity
    Diego Gravotta
    Margaret Dyson Vision Research Institute, Department of Ophthalmology, Weill Cornell Medical College, New York, NY 10065, USA
    Dev Cell 22:811-23. 2012
    ..The basolateral sorting function of AP-1A reported here establishes AP-1 as a major regulator of epithelial polarity...
  46. ncbi Direct observation of the nanoscale dynamics of membrane lipids in a living cell
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 457:1159-62. 2009
    ..The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells...
  47. ncbi Nanotubular highways for intercellular organelle transport
    Amin Rustom
    Interdisciplinary Center of Neuroscience IZN, Institute of Neurobiology, University of Heidelberg, INF 364, Heidelberg 69120, Germany
    Science 303:1007-10. 2004
    ..Accordingly, we propose a novel biological principle of cell-to-cell interaction based on membrane continuity and intercellular transfer of organelles...
  48. pmc Membrane fission is promoted by insertion of amphipathic helices and is restricted by crescent BAR domains
    Emmanuel Boucrot
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Cell 149:124-36. 2012
    ..This fission mechanism gives a new framework for understanding membrane scission in the absence of mechanoenzymes such as dynamin and suggests how Arf and Sar proteins work in vesicle scission...
  49. ncbi PIN proteins perform a rate-limiting function in cellular auxin efflux
    Jan Petrasek
    Institute of Experimental Botany, The Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic
    Science 312:914-8. 2006
    ..This suggests a direct involvement of PINs in catalyzing cellular auxin efflux...
  50. pmc The Atg16L complex specifies the site of LC3 lipidation for membrane biogenesis in autophagy
    Naonobu Fujita
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 19:2092-100. 2008
    ..We propose that the Atg16L complex is a new type of E3-like enzyme that functions as a scaffold for LC3 lipidation by dynamically localizing to the putative source membranes for autophagosome formation...
  51. pmc Coordinated protein sorting, targeting and distribution in polarized cells
    Ira Mellman
    Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Nat Rev Mol Cell Biol 9:833-45. 2008
    ..The integration of these mechanisms into a complex and dynamic network is crucial for normal tissue function and is often defective in disease states...
  52. pmc A molecular network for de novo generation of the apical surface and lumen
    David M Bryant
    Department of Anatomy, University of California, San Francisco, CA 94143 2140, USA
    Nat Cell Biol 12:1035-45. 2010
    ..This Rab11a-directed network directs Cdc42-dependent apical exocytosis during lumen formation, revealing an interaction between the machineries of vesicular transport and polarization...
  53. ncbi Crystal structure of rhodopsin: A G protein-coupled receptor
    K Palczewski
    Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA
    Science 289:739-45. 2000
    ..Identification of a set of residues that mediate interactions between the transmembrane helices and the cytoplasmic surface, where G-protein activation occurs, also suggests a possible structural change upon photoactivation...
  54. pmc STIM1 clusters and activates CRAC channels via direct binding of a cytosolic domain to Orai1
    Chan Young Park
    Department of Neurobiology, Stanford University School of Medicine, CA 94305, USA
    Cell 136:876-90. 2009
    ..These studies establish a molecular mechanism for store-operated Ca(2+) entry in which the direct binding of STIM1 to Orai1 drives the accumulation and the activation of CRAC channels at ER-PM junctions...
  55. ncbi Mechanisms of antimicrobial peptide action and resistance
    Michael R Yeaman
    Division of Infectious Diseases, Harbor University of California Los Angeles Medical Center, Torrance, California, USA
    Pharmacol Rev 55:27-55. 2003
    ..From these perspectives, the intention of this review is to illustrate the contemporary structural and functional themes among mechanisms of antimicrobial peptide action and resistance...
  56. pmc HIV-1 accessory protein Vpu internalizes cell-surface BST-2/tetherin through transmembrane interactions leading to lysosomes
    Yukie Iwabu
    Department of Pathology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    J Biol Chem 284:35060-72. 2009
    ..Also, we propose the following configuration of BST-2 in tethering virions to the cell surface; each of the dimerized BST-2 molecules acts as a bridge between viral and cell membranes...
  57. ncbi The phosphoinositide 3-kinase pathway
    Lewis C Cantley
    Department of Cell Biology, Harvard Medical School and Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA 02115 5713, USA
    Science 296:1655-7. 2002
    ..The PI3K pathway is implicated in human diseases including diabetes and cancer, and understanding the intricacies of this pathway may provide new avenues for therapuetic intervention...
  58. ncbi Functions of lipid rafts in biological membranes
    D A Brown
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794 5215, USA
    Annu Rev Cell Dev Biol 14:111-36. 1998
    ..Rafts may also function in sorting and trafficking through the secretory and endocytic pathways...
  59. ncbi Crystal structure of the sodium-potassium pump
    J Preben Morth
    Centre for Membrane Pumps in Cells and Disease PUMPKIN, Danish National Research Foundation, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Nature 450:1043-9. 2007
    ..The carboxy terminus of the alpha-subunit is contained within a pocket between transmembrane helices and seems to be a novel regulatory element controlling sodium affinity, possibly influenced by the membrane potential...
  60. pmc Emerging roles for lipids in shaping membrane-protein function
    Rob Phillips
    Department of Applied Physics, California Institute of Technology, Pasadena, California 91125, USA
    Nature 459:379-85. 2009
    ..The free-energy cost of such perturbations can be estimated quantitatively, and measurements of channel gating in model systems of membrane proteins with their lipid partners are now confirming predictions of simple models...
  61. ncbi The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:401-10. 1992
    ..We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin...
  62. ncbi The fluid mosaic model of the structure of cell membranes
    S J Singer
    Science 175:720-31. 1972
    ..Such membrane transformations may be of crucial importance in the induction of an antibody response to an antigen, as well as iv other processes of cell differentiation...
  63. ncbi A modular design for the clathrin- and actin-mediated endocytosis machinery
    Marko Kaksonen
    Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
    Cell 123:305-20. 2005
    ....
  64. ncbi Dynamin: functional design of a membrane fission catalyst
    Sandra L Schmid
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Cell Dev Biol 27:79-105. 2011
    ....
  65. pmc High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor
    Vadim Cherezov
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 318:1258-65. 2007
    ....
  66. ncbi Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments
    Julien Guizetti
    Institute of Biochemistry, Department of Biology, Swiss Federal Institute of Technology Zurich ETHZ, Schafmattstrasse 18, Zurich, Switzerland
    Science 331:1616-20. 2011
    ..The identification of contractile filament helices at the intercellular bridge has broad implications for the understanding of cell division and of ESCRT-III-mediated fission of large membrane structures...
  67. pmc Influenza virus M2 protein mediates ESCRT-independent membrane scission
    Jeremy S Rossman
    Department of Biochemistry, Northwestern University, Evanston, IL 60208, USA
    Cell 142:902-13. 2010
    ..These data suggest that M2 mediates the final steps of budding for influenza viruses, bypassing the need for host ESCRT proteins...
  68. ncbi Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria
    Julia Domínguez-Escobar
    Max Planck Institute of Biochemistry, Martinsried, Germany
    Science 333:225-8. 2011
    ..Patch motility was largely powered by cell wall synthesis, and MreB polymers restricted diffusion of patch components in the membrane and oriented patch motion...
  69. pmc Mis-assembly of clathrin lattices on endosomes reveals a regulatory switch for coated pit formation
    L h Wang
    Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235
    J Cell Biol 123:1107-17. 1993
    ..These effects suggest that CADs reverse an on/off switch that controls AP-2 binding to membranes. We conclude that cells have a mechanism for switching on and off AP-2 binding during the endocytic cycle...
  70. pmc A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages
    N Araki
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 135:1249-60. 1996
    ..These findings indicate that PI 3-kinase is not necessary for receptor-mediated stimulation of pseudopod extension, but rather functions in the closure of macropinosomes and phagosomes into intracellular organelles...
  71. pmc ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis
    Stephanie Robert
    Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    Cell 143:111-21. 2010
    ....
  72. ncbi Peripheral protein quality control removes unfolded CFTR from the plasma membrane
    Tsukasa Okiyoneda
    Department of Physiology, and Groupe de Recherche Axé sur la Structure des Protéine GRASP McGill University, Montreal, Quebec H3G 1Y6, Canada
    Science 329:805-10. 2010
    ....
  73. ncbi Membrane-derived microvesicles: important and underappreciated mediators of cell-to-cell communication
    J Ratajczak
    The Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 20:1487-95. 2006
    ....
  74. ncbi Auxin inhibits endocytosis and promotes its own efflux from cells
    Tomasz Paciorek
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, 72076 Tubingen, Germany
    Nature 435:1251-6. 2005
    ....
  75. ncbi Intracellular functions of N-linked glycans
    A Helenius
    Institute of Biochemistry, , , , Switzerland
    Science 291:2364-9. 2001
    ..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
  76. ncbi Packaging of prions into exosomes is associated with a novel pathway of PrP processing
    L J Vella
    Department of Biochemistry and Molecular Biology, The University of Melbourne, Parkville, Victoria 3010, Australia
    J Pathol 211:582-90. 2007
    ..These data suggest that exosomes represent a potent pool of prion infectivity and provide a mechanism for studying prion spread and PrP processing in cells endogenously expressing PrP...
  77. ncbi Defective membrane repair in dysferlin-deficient muscular dystrophy
    Dimple Bansal
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, Iowa 52242, USA
    Nature 423:168-72. 2003
    ..Our findings show that disruption of the muscle membrane repair machinery is responsible for dysferlin-deficient muscle degeneration, and highlight the importance of this basic cellular mechanism of membrane resealing in human disease...
  78. ncbi Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein
    H Bueler
    Institut für Molekularbiologie I, Universitat Zurich, Switzerland
    Nature 356:577-82. 1992
    ..It is now feasible to determine whether mice devoid of PrPC can propagate prions and are susceptible to scrapie pathogenesis...
  79. pmc The bacterial cell envelope
    Thomas J Silhavy
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Cold Spring Harb Perspect Biol 2:a000414. 2010
    ..Threading through these layers of peptidoglycan are long anionic polymers, called teichoic acids. The composition and organization of these envelope layers and recent insights into the mechanisms of cell envelope assembly are discussed...
  80. pmc TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation
    Björn F Lillemeier
    Howard Hughes Medical Institute, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
    Nat Immunol 11:90-6. 2010
    ..This separation versus physical juxtapositioning of receptor domains and domains containing downstream signaling molecules in quiescent versus activated T cells may be a general feature of plasma membrane-associated signal transduction...
  81. pmc Exosome function: from tumor immunology to pathogen biology
    Jeffrey S Schorey
    Center for Global Health and Infectious Diseases, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    Traffic 9:871-81. 2008
    ..In this review, we summarize our understanding of exosome biogenesis but focus primarily on new insights into exosome function. We also discuss their possible use as disease biomarkers and vaccine candidates...
  82. ncbi The third helix of the Antennapedia homeodomain translocates through biological membranes
    D Derossi
    Centre National de la Recherche Scientifique Unité de Recherche Associée 1414, Ecole Normale Superieure, Paris, France
    J Biol Chem 269:10444-50. 1994
    ..Finally, substitution of two tryptophans by two phenylalanines strongly diminishes translocation, raising the possibility that the internalization of the third helix is not solely based on its general hydrophobicity...
  83. pmc HIV enters cells via endocytosis and dynamin-dependent fusion with endosomes
    Kosuke Miyauchi
    Institute of Human Virology and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Cell 137:433-44. 2009
    ..These findings imply that HIV-1 infects cells via endocytosis and envelope glycoprotein- and dynamin-dependent fusion with intracellular compartments...
  84. ncbi Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
    N Yang
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
    Nature 393:809-12. 1998
    ..Furthermore, insulin and an active form of Rac increase the activity of LIMK-1. Taken together, our results indicate that LIMK-1 participates in Rac-mediated actin cytoskeletal reorganization, probably by phosphorylating cofilin...
  85. ncbi Caspase-1 activation of lipid metabolic pathways in response to bacterial pore-forming toxins promotes cell survival
    Laure Gurcel
    Department Microbiology and Molecular Medicine, University of Geneva, 1 rue Michel Servet, CH 1211 Geneva 4, Switzerland
    Cell 126:1135-45. 2006
    ....
  86. ncbi Autophagy controls Salmonella infection in response to damage to the Salmonella-containing vacuole
    Cheryl L Birmingham
    Infection, Immunity, Injury and Repair Program, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 281:11374-83. 2006
    ..Our findings support a role for autophagy in innate immunity and demonstrate that Salmonella infection is a powerful model to study the autophagy process...
  87. pmc Simian immunodeficiency virus envelope glycoprotein counteracts tetherin/BST-2/CD317 by intracellular sequestration
    Ravindra K Gupta
    Medical Research Council Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, 46 Cleveland Street, London W1T 4JF, United Kingdom
    Proc Natl Acad Sci U S A 106:20889-94. 2009
    ..Our observations emphasize the importance of tetherin in protecting mammals against viral infection and suggest that HIV-1 Vpu inhibitors may select active envelope mutants...
  88. pmc A comprehensive comparison of transmembrane domains reveals organelle-specific properties
    Hayley J Sharpe
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Cell 142:158-69. 2010
    ..The pervasive presence of organelle-specific features among the TMDs of a particular organelle has implications for TMD prediction, regulation of protein activity by location, and sorting of proteins and lipids in the secretory pathway...
  89. pmc Repulsion of superinfecting virions: a mechanism for rapid virus spread
    Virginie Doceul
    Department of Virology, Faculty of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Science 327:873-6. 2010
    ..Additional spreading mechanisms may exist for other viruses that also spread faster than predicted by replication kinetics...
  90. pmc CXCR7 functions as a scavenger for CXCL12 and CXCL11
    Ulrike Naumann
    Institute for Research in Biomedicine, Bellinzona, Switzerland
    PLoS ONE 5:e9175. 2010
    ..The function of CXCR7 is controversial. Some studies suggest a signaling activity in mammalian cells and zebrafish embryos, while others indicate a decoy activity in fish. Here we investigated the two propositions in human tissues...
  91. pmc A core function for p120-catenin in cadherin turnover
    Michael A Davis
    Department of Cancer Biology, Vanderbilt University, Nashville, TN 37232 6840, USA
    J Cell Biol 163:525-34. 2003
    ..The data reveal a core function of p120 in cadherin complexes, and strongly predict a dose-dependent loss of E-cadherin in tumors that partially or completely down-regulate p120...
  92. pmc Retroviruses human immunodeficiency virus and murine leukemia virus are enriched in phosphoinositides
    Robin Chan
    Centre for Life Sciences CeLS, Yong Loo Lin School of Medicine, National University of Singapore, 28 Medical Drive, Level 04 21, Singapore 117607, Singapore
    J Virol 82:11228-38. 2008
    ..Overall, these results suggest that HIV-1 and other retroviruses bud from cholesterol-rich regions of the plasma membrane and exploit matrix/PI(4,5)P(2) interactions for particle release from cells...
  93. pmc Vpu enhances HIV-1 virus release in the absence of Bst-2 cell surface down-modulation and intracellular depletion
    Eri Miyagi
    Laboratory of Molecular Microbiology, Viral Biochemistry Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
    Proc Natl Acad Sci U S A 106:2868-73. 2009
    ....
  94. ncbi Membrane phosphatidylserine regulates surface charge and protein localization
    Tony Yeung
    Division of Cell Biology, Hospital for Sick Children, Toronto M5G 1X8, Canada
    Science 319:210-3. 2008
    ..More strongly cationic proteins, normally associated with the plasma membrane, relocalized to endocytic compartments when the plasma membrane surface charge decreased on calcium influx...
  95. pmc Structures and mechanisms of viral membrane fusion proteins: multiple variations on a common theme
    Judith M White
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908 0732, USA
    Crit Rev Biochem Mol Biol 43:189-219. 2008
    ..Clearly, highly divergent proteins have converged on the same overall strategy to mediate fusion, an essential step in the life cycle of every enveloped virus...
  96. pmc Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways
    J D Axelrod
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 USA
    Genes Dev 12:2610-22. 1998
    ..The PCP signal may selectively result in focal Fz activation and asymmetric relocalization of Dsh to the membrane, where Dsh effects cytoskeletal reorganization to orient prehair initiation...
  97. ncbi Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis
    Pankaj Dhonukshe
    Center for Plant Molecular Biology ZMBP, Auf der Morgenstelle 3, University of Tubingen, D 72076 Tubingen, Germany
    Curr Biol 17:520-7. 2007
    ..Our data demonstrate that clathrin-dependent endocytosis is operational in plants and constitutes the predominant pathway for the internalization of numerous plasma-membrane-resident proteins including PIN auxin efflux carriers...
  98. pmc Plasma membrane rafts play a critical role in HIV-1 assembly and release
    A Ono
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 4, Room 307, 9000 Rockville Pike, Bethesda, MD 20892-0460, USA
    Proc Natl Acad Sci U S A 98:13925-30. 2001
    ..These results identify the association of Gag with plasma membrane rafts as an important step in HIV-1 replication. These findings may lead to novel strategies for suppressing HIV-1 replication in vivo...
  99. pmc The structure of an integrin/talin complex reveals the basis of inside-out signal transduction
    Nicholas J Anthis
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    EMBO J 28:3623-32. 2009
    ..These results show key structural features that explain the ability of talin to mediate inside-out TM signalling...
  100. pmc Intercellular junction assembly, dynamics, and homeostasis
    Kathleen J Green
    Northwestern University Feinberg School of Medicine, Department of Pathology, R H Lurie Comprehensive Cancer Center, 303 E Chicago Ave Chicago, Illinois 60611, USA
    Cold Spring Harb Perspect Biol 2:a000125. 2010
    ..We will explore signaling and trafficking machineries that drive assembly and remodeling and how these mechanisms are co-opted in human disease...
  101. ncbi Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking
    Anne J Ridley
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, 91 Riding House Street, London W1W 7BS, UK
    Trends Cell Biol 16:522-9. 2006
    ....

Research Grants73

  1. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
    ..microscopy techniques and analytical approaches to study the behavior of IgE receptors in the mast cell membrane. We have discovered that the plasma membrane has a rich topography, that we believe is critical to the ..
  2. Structure and function of urea transporters
    Ming Zhou; Fiscal Year: 2013
    ..by applicant): Urea transporters (UT) are integral membrane proteins that facilitate transport of urea across cell membrane. UTs are highly expressed in many mammalian tissues, including kidney, liver, and brain...
  3. Regulation of B Cell Function by Membrane-Cytoskeletal Remodeling
    Neetu Gupta; Fiscal Year: 2012
    ..All these events are associated with dynamic morphological and compositional reorganization of the B cell membrane. Membrane remodeling requires membrane uncoupling from the underlying cortical cytoskeleton but its regulation ..
  4. Patient-Derived Stem Cells for Phosphoproteomic Profiling Neuropsychopathology
    Dieter A Wolf; Fiscal Year: 2011
    ..also suggested that lithium administration reduces translocation of Protein Kinase C from the cytosol to the cell membrane {Hahn, 1999 #158}, &inhibits GSK3 activity in mice (Catapano &Manji, 2008)...
  5. Regulation of Cell Signaling by Human Biliverdin Reductase
    Mahin D Maines; Fiscal Year: 2013
    ..that govern adaptive and protective regulatory mechanisms of the cell by relaying signals initiated at the cell membrane to the nucleus of the cell for generating appropriate response...
  6. TARGETING THE MARCKS-FAMILY PROTEINS TO INHIBIT NEUTROPHIL MIGRATION
    Mary K Sheats; Fiscal Year: 2013
    ..Alanine-Rich C-Kinase Substrate (MARCKS) family of proteins, MARCKS and MARCKS-like 1 (MARCKSL1), are known cell membrane and actin binding proteins regulated by protein kinase C (PKC) phosphorylation and calcium/calmodulin binding...
  7. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2013
    ..These properties stem from the way S. mutans inserts proteins into its cytoplasmic membrane. This research will explain how proteins get into and through the membrane of this and other related pathogens. ..
  8. Obesity: neural control of body weight and metabolism
    Barry E Levin; Fiscal Year: 2013
    ..regulators of leptin sensitivity and that altered regulation of trafficking of the leptin receptor to the cell membrane may be a critical determinant of the inborn leptin resistance of DIO rats...
  9. Neuropeptide regulation of neurohypophyseal function
    Francis W Flynn; Fiscal Year: 2012
    ..a hyperosmotic challenge NK3R expressed by VP magnocellular neurons were shown to be translocated from the cell membrane to the cell nucleus by confocal microscopy, immuno-electron microscopy and Western blot...
  10. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    Quentin J Baca; Fiscal Year: 2010
    ..it is clear that a critical aspect of activation is the regulation of receptor mobility in the plane of the T cell membrane. The focus of this proposal is to investigate biologically important mechanisms of membrane receptor mobility ..
  11. MECHANISM OF DRUG TRANSPORT IN LUNG CANCER CELLS
    Sanjay Awasthi; Fiscal Year: 2011
    ..These proteins are key elements of cell membrane plasticity, actin-cytoskeletal regulation and cell migration (cancer cell invasion and metastasis), receptor-..
  12. Mechanistic Characterization and Genetic Analysis of Systemic Iron Homeostasis
    Xin Du; Fiscal Year: 2011
    ..of TMPRSS6, hoping to gain insight on the proximal events in signal transduction initiated by TMPRSS6 on the cell membrane;2...
  13. Mechanisms of Cochlear Oxidative Stress Injury and Strategies to Protect Hearing
    Michael J Brenner; Fiscal Year: 2013
    ..This work will better define the role of cell membrane channels, transcription factors, and other molecular targets in gentamicin-induced hearing loss...
  14. EFFECT OF LIPIDS ON VASCULAR GRAFT HEALING
    Linda M Graham; Fiscal Year: 2013
    ..Old and lysoPC increase cellular production of reactive oxygen species, increase cell membrane fluidity, and open ion channels. These effects can inhibit EC migration...
  15. Gene Transcription in SLE
    George C Tsokos; Fiscal Year: 2012
    ..It is hypothesized that cell membrane-initiated signaling processes lead to increased expression and activation of transcription repressors and ..
  16. Site-directed Chemotherapy for Breast Cancer using Novel Angiogenesis Inhibitor
    Shaker A Mousa; Fiscal Year: 2010
    ..acid (tetrac), a deaminated thyroid hormone analog, capable of preventing the binding of T4 to 1vB3 at the cell membrane exerted profound inhibitory effects on cellular proliferation and angiogenesis...
  17. Varicella zoster virus: molecular controls of cell fusion-dependent pathogenesis
    Ann Arvin; Fiscal Year: 2013
    ..along with the gH/gL heterodimer is known to be critical for fusion of the virion envelope with the target cell membrane during herpesvirus entry...
  18. Inhibitors of the Y. pestis cell surface plasminogen activator for biodefense
    Marjorie H Barnes; Fiscal Year: 2010
    ..In preliminary studies, reliable assays were developed for inhibitors of cell membrane-associated Pla. Recombinant E. coli strains producing active outer- membrane anchored Y...
  19. Molecular physiology of voltage-gated Ca2+ channels
    Jian Yang; Fiscal Year: 2013
    ..By studying a novel form of activity-dependent fragmentation of VGCCs on the cell membrane, this work may lead to a better understanding of VGCC function and regulation in physiological and ..
  20. Functional Studies of Variants of the Bipolar Risk Gene CACNA1C in Human Neurons
    JEN PAN; Fiscal Year: 2013
    ..ion channels are expressed in all excitable and many non-excitable cells to transduce electric signals at the cell membrane into chemical potentials of calcium influx...
  21. Identification of Mechanoresponsive Promoter Elements in Chondrogenesis
    Dominik R Haudenschild; Fiscal Year: 2013
    ..Traditional studies of mechanotransduction events have focused on cell membrane-associated proteins and cytosolic signal transduction proteins...
  22. Inhibition of angiogenesis by thrombospondin-1 (A2)
    JOHN W LAWLER; Fiscal Year: 2012
    ..We hypothesize that the CD36/VEGFR2/integrin complexes in the endothelial cell membrane mediate 3TSR-induced apoptosis and facilitates cross-talk between pro- and anti-angiogenic signal transduction ..
  23. GENETICS AND VIRULENCE OF PNEUMOCOCCAL CAPSULAR TYPES
    JANET L YOTHER; Fiscal Year: 2012
    ..By understanding the mechanisms involved in this process, we may be better able to develop means for treating and preventing these infections. ..
  24. Steroid-Based Contrast Agents for Magnetic Resonance Imaging of Endocrine Disease
    Thomas J Meade; Fiscal Year: 2013
    ..have identified gadolinium (Gd)-conjugated progesterone derivatives as compounds capable of traversing the cell membrane, binding to progesterone receptors, initiating gene transcription, and enhancing contrast in mammalian tissues ..
  25. Mechanistic Studies of HCV E2
    Joseph Marcotrigiano; Fiscal Year: 2013
    ..as pestiviruses, alphaviruses, and other flaviviruses, HCV infection requires fusion between viral and host cell membrane before the viral genome can be released into the host cell cytosol...
  26. Liver Cell Membrane Protein-Expression and Function
    Allan W Wolkoff; Fiscal Year: 2012
    This is a Program Project to continue investigation of the functions and trafficking of specific liver cell membrane proteins. Four projects involving 15 faculty investigators representing 6 academic departments are proposed...
  27. Single RNA sensitive probes for studying viral replication and budding
    Philip J Santangelo; Fiscal Year: 2013
    ..In preliminary experiments, MTRIPs, when delivered via cell membrane permeabilization with streptolysin O (SLO), bound specifically and rapidly to RNA (<10 minutes) and allowed ..
  28. Mechanisms of Pseudomonas Resistance to Membrane Permeabilization by SP-A
    Gee W Lau; Fiscal Year: 2012
    ..We propose three aims to examine the mechanisms by which flagellum, PchA and PtsP pathways maintain LPS and cell membrane integrity, and regulate the bacterial processes that inactivate SP-A, to confer resistance to killing by SP-A-..
  29. Sphingolipids in Acute Kidney Injury
    MARK DOUGLAS OKUSA; Fiscal Year: 2013
    ..to a series of bioactive intermediates including sphingosine 1-phosphate (S1P) following stimulation of cell membrane receptors or injury to plasma membranes...
  30. Novel Erythroid Cell Membrane Protein
    Emery H Bresnick; Fiscal Year: 2010
    ....
  31. The Role of CRACM1 in the Pathogenesis of Systemic Lupus Erythematosus
    Peter C Chien; Fiscal Year: 2013
    ..of mast cell signaling and identified Calcium-Release-Activated Calcium Channel Modulator 1 (CRACM1) as a key cell membrane pore responsible for calcium flux...
  32. VOLUME REGULATION IN NORMAL AND CATARACTOUS LENSES
    Richard T Mathias; Fiscal Year: 2013
    ..Osmotic studies of isolated fiber cell membrane vesicles will determine water permeability and its regulation by pH and calcium...
  33. INTRACOCHLEAR ELECTROCHEMICAL GRADIENTS
    Alexander A Spector; Fiscal Year: 2012
    ..proteins that facilitate the movement of anions (negatively charged ions such as chloride) through the cell membrane. The structure and function of prestin are analyzed and the contributions of the lateral wall membranes to the ..
  34. Molecular elucidation of the CBM complex in NF-kappaB activation by antigen recep
    Hao Wu; Fiscal Year: 2013
    ..These translocations likely activate NF-kB through MALT1 and Bcl10 overexpression. Collectively, these studies suggest that the CBM complex is an attractive therapeutic target neoplastic disorders of the lymphocytes. ..
  35. Genetics and Genomics of the yidC paralogs in Streptococcus mutans
    SARA MARIE RASER PALMER; Fiscal Year: 2013
    ..The data generated will provide essential new information that can be used to design novel and more effective therapies to combat dental caries and other bacterial infections. ..
  36. Computational and Cell Culture Models for Mucus Clearance
    Richard Superfine; Fiscal Year: 2010
    ..The particulates, pathogens and allergens are in a diffusive race for the cell membrane before they can be cleared by the entraining flow of mucus...
  37. Acquisition of a TIRF/Widefield fluorescence microscope for cell biology and neur
    Bryon D Grove; Fiscal Year: 2013
    ..an imaging technology that allows very precise realtime fluorescent imaging of dynamic molecular events at the cell membrane surface, and state of the art widefield fluorescence microscopy are necessary for investigators of the ..
  38. HA Surface Presented Yeast Flu Vaccine and Its Enhancement by CD154 Codisplay
    Kaiming Ye; Fiscal Year: 2012
    ..facilitate the recognition of antigens by host immune systems and mediate an immunoadjuvant effect with yeast cell membrane components...
  39. Study of the HFE Transferrin Receptor interaction in mammalian iron homeostasis
    Paul Schmidt; Fiscal Year: 2010
    ..class l-like gene that forms a protein-protein complex with TFR soon after synthesis and is transported to the cell membrane. This interaction causes a conformational change in TFR1, reducing the affinity of the receptor for holo-..
  40. Calcium-sensing Receptor and Keratinocyte Differentiation
    Chia Ling Tu; Fiscal Year: 2013
    ..The CaR not only localizes on the cell membrane to detect changes in Ca2+o, but also forms a protein complex with modulators of Ca2+i stores and store-..
  41. 2011 Cell-Cell Fusion Gordon Research Conference and Seminar
    NANCY RYAN GRAY; Fiscal Year: 2011
    ..be covered by the invited speakers, along with the latest progress on the basic mechanisms and applications of cell membrane fusion in vitro and in vivo...
  42. Legionella pneumophila developmental cycle & virulence
    PAUL STOKES HOFFMAN; Fiscal Year: 2011
    ..These studies should also provide insights relevant to other opportunistic environmental pathogens including oxiella, Burkholderia and species of Mycobacterium. ..
  43. A NEW TECHNIQUE FOR TREATING HEMORRHAGIC SHOCK
    Sufan Chien; Fiscal Year: 2013
    ..Furthermore, it i well known that highly charged molecules like ATP do not normally cross the cell membrane. We have developed a technique for intracellular Mg-ATP delivery (named ATP- vesicles or VitaSolTM)...
  44. INFIDELITY OF CYTOSINE METHYLATION AND HUMAN CANCER
    Lawrence C Sowers; Fiscal Year: 2013
    ..In human tissues, reactive molecules generated by immune cells must cross the cell membrane and enter the nucleus in order to react with the DNA...
  45. A Novel IgE Cancer Therapeutic Specific for the Epithelial Membrane Protein-2
    TRACY RUTH DANIELS-WELLS; Fiscal Year: 2013
    ..membrane protein involved in cell adhesion, invasion, and signal transduction through the regulation of cell membrane composition...
  46. Lipid Translocating Glycosyltransferases in Congenital Neurological Disease
    PETER AB ORLEAN; Fiscal Year: 2010
    ..pathways leading to these protein modifications include steps in which sugar molecules are moved across a cell membrane and then attached to protein, and enzymes involved in seven such steps are affected in congenital ..
  47. Membrane skeleton regulation of cell shape and interactions in lens development
    Velia M Fowler; Fiscal Year: 2013
    ..of a long-range spectrin-actin network linked to its attachment proteins (NrCAM, N-cadherin) forms a fiber cell membrane subdomain that excludes connexins, thereby 'corralling'the connexins into the large gap junction ..
  48. Alcohol increases tight junction permeability &disrupts translocation of ZO-1
    Samantha Simet; Fiscal Year: 2012
    ..We have recently observed that alcohol impairs ZO-1 and claudin-1 translocation to the cell membrane and increases tight junction permeability resulting in "leakier" airway epithelial cells...
  49. The Role of Intracellular Metabotropic Glutamate Receptor 5 at the Synapse
    CAROLYN ANN PURGERT; Fiscal Year: 2012
    ..The native ligand of mGluR5, glutamate, can cross the cell membrane via various transporters and exchangers;therefore, glutamate released at a synapse can activate both cell ..
  50. The S1P1 receptor regulates sarcomere assembly and cardiac development
    Roland S Wu; Fiscal Year: 2013
    ..S1P is a product of cell membrane metabolism and found ubiquitously throughout the body...
  51. Regulation of Pore Formation by Type III Secretion System
    Gloria I Viboud; Fiscal Year: 2010
    ..Wild type Yersinia does not disrupt the integrity of the host cell membrane during the translocation process...
  52. Pneumocystis carinii: Macrophage &Epithelial Activation
    Andrew H Limper; Fiscal Year: 2013
    ..We also observed that host cell membrane lactosylceramide strongly participates in inflammatory activation in response to Pc ? -1,6 glucans...
  53. Studies of YlxM of Streptococcus mutans, a newly identified component of the Sign
    MATTHEW LAWRENCE WILLIAMS; Fiscal Year: 2013
    ..Additionally this research represents the basis of a doctoral dissertation project. Hence, this fellowship application will assist in trainin a student with an interest in oral health research to become an independent investigator. ..
  54. GENETIC STUDIES OF GLUTAMINE SYNTHETASE IN BACTERIA
    SYDNEY GOVONS KUSTU; Fiscal Year: 2010
    ..New biochemical pathways are rare and their characterization is useful in the annotation of genomes and in studies of the metabolic and regulatory interlocks important to cellular self-replication. ..
  55. Facilitating large scale biomass generation: acquisition of an automated 100 L fe
    Roger D Kornberg; Fiscal Year: 2010
    ..cell adhesion through the surface receptor, and binding of regulatory proteins to the SNAREs responsible for cell membrane fusion. All of this work entails X-rays crystallographic analysis, and depends on bacterial fermentation...
  56. Molecular Mechanisms of Albumin Trafficking in Podocytes
    Judith T Blaine; Fiscal Year: 2013
    ..Specifically, we will test the hypothesis that albumin endocytosis occurs at the basal cell membrane and is mediated by megalin and that albumin degradation occurs in the lysosome...
  57. 12-HETrE regulation of platelets
    MICHAEL ALLAN HOLINSTAT; Fiscal Year: 2013
    ..Since DGLA is highly expressed in the phospholipids of the cell membrane and 12-LOX metabolites can be released into circulation in [unreadable]M concentrations, delineating the ..
  58. THYROID PHYSIOLOGY STUDIES OF INHERITED DISORDERS
    Samuel Refetoff; Fiscal Year: 2013
    ..mediation of thyroid hormone (TH) effects by studying syndromes of reduced sensitivity to TH due to defects in cell membrane transport, metabolism and action...
  59. Mechanism of Apoptosis and Inhibition of Tumor Progression and Metastasis by Par-
    Vivek M Rangnekar; Fiscal Year: 2012
    ..e., from the endoplasmic reticulum to the Golgi, and then to the cell membrane)...
  60. Membrane Protein Trafficking in Photoreceptors
    Wolfgang Baehr; Fiscal Year: 2013
    ..emerge from the trans-Golgi network (TGN) and transport to the base of the cilium where fusion with the cell membrane occurs. Finally, cargo is assembled for intraflagellar transport...
  61. Recombinant and Chemo-/Bio-Orthogonal Synthesis of Liposomal Thrombomodulin
    Xue Long Sun; Fiscal Year: 2013
    ..in which lipid composition closely resembles that of cell membranes, have been extensively studied as cell membrane model as well as carrier for delivering certain vaccines, enzymes, drugs, or genes to their active sites...
  62. Oxidative and nitrosative stress resistance of Cryptococcus neoformans
    Jennifer K Lodge; Fiscal Year: 2010
    ..of the thiol peroxidase and that Tsa1 is localized in the cell periphery, the precise localization (cell wall, cell membrane, membrane associated, or mitochondrial) of the thiol peroxidases and thioredoxin and the potential for ..
  63. MAST CELL MEMBRANE GLYCOLIPID EXPRESSION AND FUNCTION
    Michael Raizman; Fiscal Year: 1991
    ..proposal is to develop basic research skills in immunology and biochemistry through the investigation of mast cell membrane glycosphingolipids...
  64. Analysis of Polyhydroxyalkanoate Inclusion Biogenesis
    Douglas Dennis; Fiscal Year: 2009
    ..The purpose of this research is to test this preliminary model in hopes of adapting the strategy of biogenesis for medical applications in which inclusions could be used as drug delivery vehicles. ..
  65. CELLULAR AND MOLECULAR STUDIES OF VESTIBULAR HAIR CELLS
    MANNING CORREIA; Fiscal Year: 2007
    The long-term goal of this project is to understand the mechanisms by which ion channels in the vestibular hair cell membrane modulate the cell's membrane potential...
  66. Role of Red Cell Membrane in Malaria Parasite Release
    Manjit Hanspal; Fiscal Year: 2006
    ..FP-2 mediates the cleavage of erythrocyte membrane skeletal proteins that are vital to the stability of red cell membrane, thus modulating the parasite release in vivo...
  67. Noninvasive assessment of skeletal muscle integrity and function with statin use
    Jill Slade; Fiscal Year: 2009
    ..Statin effects include myocyte apoptosis, decreased cell membrane integrity, mitochondrial myopathy, diminished cytoskeletal integrity and diminished muscle repair...
  68. Real-time microviscosity measurement tools for the cell
    Mark A Haidekker; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Microviscosity in the cell membrane and the cell cytoplasm are important parameters in cell signaling and disease...
  69. TRANSPORT OF AN E COLI VIRULENCE FACTOR--THE K1 CAPSULE
    Richard Silver; Fiscal Year: 2000
    ....
  70. PROTEIN KINASE C MEDIATION OF EXOCRINE PANCREATIC SECRET
    Dexter Louie; Fiscal Year: 1992
    ..We will demonstrate that translocation of PKC from cytosol to the cell membrane is an essential step for the initial release of amylase and that this is mediated by increased intracellular ..
  71. REGULATION OF NEURONAL CALCIUM CHANNELS
    JEFFRY LANSMAN; Fiscal Year: 2000
    ..metabotropic glutamate receptors (mGluRs) elevate intracellular Ca2+ by increasing Ca2+ influx across the cell membrane in addition to releasing this cation from intracellular stores via an increase in inositol triphosphate...
  72. GENETIC INTERACTIONS IN RED CELL MEMBRANE DISEASE
    LUANNE PETERS; Fiscal Year: 2003
    ..1) interactions between the membrane-spanning domain of band 3 and plasma membrane lipids stabilize the red cell membrane. The ability of the membrane-spanning and cytoplasmic domains of band 3 to independently stabilize the RBC ..
  73. Identification small molecule inhibitors of the bacterial morphogenetic complex
    JAMES GOBER; Fiscal Year: 2009
    ....