mononuclear leukocytes

Summary

Summary: Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.

Top Publications

  1. ncbi Quantitative expression of toll-like receptor 1-10 mRNA in cellular subsets of human peripheral blood mononuclear cells and sensitivity to CpG oligodeoxynucleotides
    Veit Hornung
    Department of Internal Medicine, Division of Clinical Pharmacology, University of Munich, Munich, Germany
    J Immunol 168:4531-7. 2002
  2. ncbi Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8
    Florian Heil
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Trogerstr 9, D 81675 Munich, Germany
    Science 303:1526-9. 2004
  3. pmc Tumor necrosis factor alpha protects against lethal West Nile virus infection by promoting trafficking of mononuclear leukocytes into the central nervous system
    Bimmi Shrestha
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA
    J Virol 82:8956-64. 2008
  4. ncbi HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor
    Y Feng
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases NIAID, NIH, Bethesda, MD 20892, USA
    Science 272:872-7. 1996
  5. pmc Interferon-inducible gene expression signature in peripheral blood cells of patients with severe lupus
    Emily C Baechler
    Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:2610-5. 2003
  6. ncbi Coordinate overexpression of interferon-alpha-induced genes in systemic lupus erythematosus
    Kyriakos A Kirou
    Hospital for Special Surgery, and Weill Medical College of Cornell University, New York, New York 10021, USA
    Arthritis Rheum 50:3958-67. 2004
  7. pmc Cellular tumor necrosis factor, gamma interferon, and interleukin-6 responses as correlates of immunity and risk of clinical Plasmodium falciparum malaria in children from Papua New Guinea
    Leanne J Robinson
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, Australia
    Infect Immun 77:3033-43. 2009
  8. ncbi Protective vaccination against experimental canine visceral leishmaniasis using a combination of DNA and protein immunization with cysteine proteinases type I and II of L. infantum
    Sima Rafati
    Molecular Immunology Lab, Department of Immunology, Pasteur Institute of Iran, P O Box 11365 6699, Tehran, Iran
    Vaccine 23:3716-25. 2005
  9. ncbi Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G: a possible role in the resistance to HIV of HIV-exposed seronegative individuals
    Mara Biasin
    Department of Immunology, Dipartimento di Scienze Precliniche Laboratorio Interdisciplinare Tecnologie Avanzate Vialba, Milano University Medical School, Milano, Italy
    J Infect Dis 195:960-4. 2007
  10. ncbi Transcription factor and drug-metabolizing enzyme gene expression in lymphocytes from healthy human subjects
    Gerard Siest
    Equipe Institut National de la Santé et de la Recherche Médicale Génétique Cardiovasculaire CIC 9501, Faculte de Pharmacie, Universite Henri Poincare Nancy I, Nancy, France
    Drug Metab Dispos 36:182-9. 2008

Research Grants

  1. PATHOPHYSIOLOGY OF THE ENDOTHELIUM
    Francis W Luscinskas; Fiscal Year: 2013
  2. ROLE OF MONONUCLEAR LEUKOCYTES IN IMMUNITY
    Samuel Silverstein; Fiscal Year: 2009
  3. Vascular adhesion protein-1 (VAP-1) as a therapeutic target in stroke
    DALE ALAN PELLIGRINO; Fiscal Year: 2013
  4. Alpha M Beta 2 integrin blockade for acute inflammatory neuropathies
    EROBOGHENE EKAMERENO UBOGU; Fiscal Year: 2013
  5. Mechanisms of risk for sulfoniamide hypersensitivity
    Lauren A Trepanier; Fiscal Year: 2013
  6. Fibronectin peptide antagonism for chronic inflammatory neuropathies
    EROBOGHENE EKAMERENO UBOGU; Fiscal Year: 2012
  7. alpha4beta1 Intergrin Signaling In Immunity And Arthritis
    GREGG JOSHUA SILVERMAN; Fiscal Year: 2010
  8. MONONUCLEAR LEUKOCYTES AND IMMUNITY
    Samuel Silverstein; Fiscal Year: 2003
  9. DIETARY LIPIDS--EFFECTS ON LIPID-LIPOPROTEIN METABOLISM
    D Illingworth; Fiscal Year: 1991
  10. Carbon monoxide therapy for severe pulmonary arterial hypertension
    Roberto F Machado; Fiscal Year: 2013

Detail Information

Publications263 found, 100 shown here

  1. ncbi Quantitative expression of toll-like receptor 1-10 mRNA in cellular subsets of human peripheral blood mononuclear cells and sensitivity to CpG oligodeoxynucleotides
    Veit Hornung
    Department of Internal Medicine, Division of Clinical Pharmacology, University of Munich, Munich, Germany
    J Immunol 168:4531-7. 2002
    ..In addition, selective regulation of TLR7 expression in PDC and B cells by CpG ODN revealed TLR7 as a candidate TLR potentially involved in modulating the recognition of CpG motifs...
  2. ncbi Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8
    Florian Heil
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Trogerstr 9, D 81675 Munich, Germany
    Science 303:1526-9. 2004
    ..These data suggest that ssRNA represents a physiological ligand for TLR7 and TLR8...
  3. pmc Tumor necrosis factor alpha protects against lethal West Nile virus infection by promoting trafficking of mononuclear leukocytes into the central nervous system
    Bimmi Shrestha
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA
    J Virol 82:8956-64. 2008
    ..Our data are most consistent with a model in which interaction of TNF-alpha with TNF-R1 protects against WNV infection by regulating migration of protective inflammatory cells into the brain during acute infection...
  4. ncbi HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor
    Y Feng
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases NIAID, NIH, Bethesda, MD 20892, USA
    Science 272:872-7. 1996
    ..Fusin messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Fusin acted preferentially for T cell line-tropic isolates, in comparison to its activity with macrophagetropic HIV-1 isolates...
  5. pmc Interferon-inducible gene expression signature in peripheral blood cells of patients with severe lupus
    Emily C Baechler
    Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:2610-5. 2003
    ..These results provide insights into the genetic pathways underlying SLE, and identify a subgroup of patients who may benefit from therapies targeting the IFN pathway...
  6. ncbi Coordinate overexpression of interferon-alpha-induced genes in systemic lupus erythematosus
    Kyriakos A Kirou
    Hospital for Special Surgery, and Weill Medical College of Cornell University, New York, New York 10021, USA
    Arthritis Rheum 50:3958-67. 2004
    ....
  7. pmc Cellular tumor necrosis factor, gamma interferon, and interleukin-6 responses as correlates of immunity and risk of clinical Plasmodium falciparum malaria in children from Papua New Guinea
    Leanne J Robinson
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, Australia
    Infect Immun 77:3033-43. 2009
    ..falciparum may therefore be important correlates of immunity and risk of symptomatic malaria episodes and thus warrant detailed investigation in relation to the development and implementation of effective vaccines...
  8. ncbi Protective vaccination against experimental canine visceral leishmaniasis using a combination of DNA and protein immunization with cysteine proteinases type I and II of L. infantum
    Sima Rafati
    Molecular Immunology Lab, Department of Immunology, Pasteur Institute of Iran, P O Box 11365 6699, Tehran, Iran
    Vaccine 23:3716-25. 2005
    ..We propose that the combination of DNA and recombinant protein vaccination using CPs could be instrumental to control (VL) in dogs...
  9. ncbi Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G: a possible role in the resistance to HIV of HIV-exposed seronegative individuals
    Mara Biasin
    Department of Immunology, Dipartimento di Scienze Precliniche Laboratorio Interdisciplinare Tecnologie Avanzate Vialba, Milano University Medical School, Milano, Italy
    J Infect Dis 195:960-4. 2007
    ..Higher APOBEC3G expression correlated with a reduced susceptibility of PBMCs to in vitro infection with the HIV-1(Ba-L) R5 strain. APOBEC3G could be important in modulating in vivo susceptibility to sexually transmitted HIV infection...
  10. ncbi Transcription factor and drug-metabolizing enzyme gene expression in lymphocytes from healthy human subjects
    Gerard Siest
    Equipe Institut National de la Santé et de la Recherche Médicale Génétique Cardiovasculaire CIC 9501, Faculte de Pharmacie, Universite Henri Poincare Nancy I, Nancy, France
    Drug Metab Dispos 36:182-9. 2008
    ..The expression of these genes in lymphocytes could be a useful tool for further studying the physiological and pathological variations of DME and TF related to environment, to drug intake, and to cardiovascular metabolic cycles...
  11. ncbi Association of early interferon-gamma production with immunity to clinical malaria: a longitudinal study among Papua New Guinean children
    Marthe C D'Ombrain
    Infection and Immunity Division, The Walter and Eliza Hall Institute of Medical Research, University of Melbourne, Parkville, Victoria, Australia
    Clin Infect Dis 47:1380-7. 2008
    ..The question remains whether similar heterogeneity is observed in malaria-exposed individuals and whether high, medium, or low IFN-gamma responsiveness is differentially associated with protective immunity or morbidity...
  12. pmc A small molecule that inhibits the interaction of paxillin and alpha 4 integrin inhibits accumulation of mononuclear leukocytes at a site of inflammation
    Christiane Kummer
    Department of Medicine, University of California at San Diego, La Jolla, California 92093, USA
    J Biol Chem 285:9462-9. 2010
    ..Administration of this compound to mice led to impaired recruitment of mononuclear leukocytes to a site of inflammation in vivo, whereas an isomer that does not inhibit the alpha 4-paxillin ..
  13. ncbi Unblinded pilot study of autologous transplantation of bone marrow mononuclear cells in patients with thromboangiitis obliterans
    Koji Miyamoto
    Department of Medicine, National Cardiovascular Center, 5 7 1 Fujishiro dai, Suita, Osaka 565 8565, Japan
    Circulation 114:2679-84. 2006
    ..The purpose of this study was to assess the long-term safety and efficacy of bone marrow mononuclear cell transplantation in patients with thromboangiitis obliterans...
  14. ncbi Generation of highly suppressive adaptive CD8(+)CD25(+)FOXP3(+) regulatory T cells by continuous antigen stimulation
    Milada Mahic
    Biotechnology Centre and Centre for Molecular Medicine Norway, Nordic EMBL Partnership, University of Oslo, Norway
    Eur J Immunol 38:640-6. 2008
    ..However, neither blocking antibodies to CTLA-4, CD80 nor CD86 could reverse CD8(+) T(R)-mediated suppression, indicating that other mechanism(s) must be employed by these cells...
  15. pmc Regulated expression of microRNAs in normal and polycythemia vera erythropoiesis
    Hana Bruchova
    Hematology Division, Department of Pathology, University of Utah, School of Medicine, Salt Lake City, UT 84132, USA
    Exp Hematol 35:1657-67. 2007
    ..Because microRNAs (miRNAs) play a regulatory role in hematopoiesis, we studied miRNA expressions in PV and normal erythropoiesis...
  16. pmc Mononuclear leukocytes bind to specific hyaluronan structures on colon mucosal smooth muscle cells treated with polyinosinic acid:polycytidylic acid: inter-alpha-trypsin inhibitor is crucial to structure and function
    Carol A De La Motte
    Department of Colorectal Surgery and Immunology, The Cleveland Clinic Foundation, Cleveland, Ohio, USA
    Am J Pathol 163:121-33. 2003
    ..treatment of human colon M-SMCs in vitro increases cell surface hyaluronan (HA), and nonactivated mononuclear leukocytes bind to virus-induced HA structures by interactions that involve the HA-binding receptor CD44...
  17. ncbi Circulating mononuclear cells in the obese are in a proinflammatory state
    Husam Ghanim
    Division of Endocrinology, Diabetes, and Metabolism, State University of New York at Buffalo, NY, USA
    Circulation 110:1564-71. 2004
    ....
  18. pmc DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets
    Chawaree Chaipan
    Nikolaus Fiebiger Center for Molecular Medicine, University Erlangen Nurnberg, Glückstrasse 6, 91054 Erlangen, Germany
    J Virol 80:8951-60. 2006
    ..Our results identify CLEC-2 as a novel HIV-1 attachment factor and provide evidence that platelets capture and transfer infectious HIV-1 via DC-SIGN and CLEC-2, thereby possibly facilitating HIV-1 dissemination in infected patients...
  19. pmc Natural killer cells in HIV-1 infection: dichotomous effects of viremia on inhibitory and activating receptors and their functional correlates
    Domenico Mavilio
    Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:15011-6. 2003
    ..This phenomenon is not attributed to a direct HIV-1 infection of NK cells; thus, this study may provide insight into the mechanisms of impaired host defenses in HIV-1 viremic patients...
  20. ncbi Microbial Toll-like receptor ligands differentially regulate CXCL10/IP-10 expression in fibroblasts and mononuclear leukocytes in synergy with IFN-gamma and provide a mechanism for enhanced synovial chemokine levels in septic arthritis
    Paul Proost
    Laboratory of Molecular Immunology, Rega Institute, K U Leuven, Leuven, Belgium
    Eur J Immunol 33:3146-53. 2003
    ....
  21. ncbi The envelope protein of a human endogenous retrovirus-W family activates innate immunity through CD14/TLR4 and promotes Th1-like responses
    Alexandre Rolland
    Laboratoire d Immunochimie, Institut National de la Santé et de la Recherche Médicale Unité 548, Commissariat a l Energie Atomique, Grenoble, France
    J Immunol 176:7636-44. 2006
    ..Finally, ENV-SU endowed dendritic cells with the capacity to support a Th1-like type of Th cell differentiation. The data are discussed in the context of immune responses and chronic proinflammatory disorders...
  22. pmc Highly sensitive methods based on seminested real-time reverse transcription-PCR for quantitation of human immunodeficiency virus type 1 unspliced and multiply spliced RNA and proviral DNA
    Alexander O Pasternak
    Department of Medical Microbiology, Laboratory of Experimental Virology, Center for Infection and Immunity Amsterdam, Academic Medical Center, University of Amsterdam, Meibergdreef 15, Amsterdam 1105 AZ, The Netherlands
    J Clin Microbiol 46:2206-11. 2008
    ..These easy-to-perform methods can be widely used in research, including clinical studies, to monitor intracellular processes of virus replication...
  23. ncbi The prolonged culture of human immunodeficiency virus type 1 in primary lymphocytes increases its sensitivity to neutralization by soluble CD4
    Pavel Pugach
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA
    Virology 321:8-22. 2004
    ..The loss of neutralization resistance is of no relevance under in vitro conditions, but NAbs would presumably be a counter-selection pressure against such adaptive changes in vivo, at least when the humoral immune response is intact...
  24. pmc Relationships between infectious titer, capsid protein levels, and reverse transcriptase activities of diverse human immunodeficiency virus type 1 isolates
    Andre J Marozsan
    Division of Infectious Diseases, BRB 1029, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    J Virol 78:11130-41. 2004
    ..Finally, the replication efficiency or fitness of both the NSI/R5 and SI/X4 HIV-1 isolates was not linked to the titers of the virus stocks...
  25. ncbi Impact of 5 years of maximally successful highly active antiretroviral therapy on CD4 cell count and HIV-1 DNA level
    Jean Paul Viard
    Service d immunologie clinique, Universite Rene Descartes, Faculte de Medecine Necker Enfants Malades, Paris, France
    AIDS 18:45-9. 2004
    ..e., constant plasma HIV-1 RNA load suppression...
  26. pmc Immunization with H1, HASPB1 and MML Leishmania proteins in a vaccine trial against experimental canine leishmaniasis
    J Moreno
    WHO Collaborating Centre for Leishmaniasis, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Spain
    Vaccine 25:5290-300. 2007
    ..The results demonstrate that HASPB1 and H1 antigens in combination with Montanide were able to induce partial protection against canine leishmaniasis, even under extreme experimental challenge conditions...
  27. ncbi Role of mononuclear cells and inflammatory cytokines in pancreatic cancer-related cachexia
    Marc E Martignoni
    Department of General Surgery, University of Heidelberg, Germany
    Clin Cancer Res 11:5802-8. 2005
    ..In this study, we investigated which factors are associated with the cachectic status of the patients and evaluated cachexia-promoting capacity of cancer and inflammatory cells...
  28. ncbi Interferon-alpha response to swine arterivirus (PoAV), the porcine reproductive and respiratory syndrome virus
    E Albina
    CNEVA Ploufragan, Unité de Virologie et Immunologie Porcines, France
    J Interferon Cytokine Res 18:485-90. 1998
    ..This suppressive effect was lost when the virus was inactivated by UV light. Our results suggest that downregulation of IFN-alpha production may play an important part in enabling PoAV to replicate in cell cultures and in pigs...
  29. ncbi Peripheral blood mononuclear cell gene expression profiles identify emergent post-traumatic stress disorder among trauma survivors
    R H Segman
    Department of Psychiatry, Hadassah University Hospital, Jerusalem, Israel
    Mol Psychiatry 10:500-13, 425. 2005
    ..Replications in larger samples, as well as studies focusing on specific markers within the signatures discovered, are warranted to confirm and extend the diagnostic utility and pathogenetic implications of our results...
  30. ncbi Effects of docosahexaenoic acid-rich n-3 fatty acid supplementation on cytokine release from blood mononuclear leukocytes: the OmegAD study
    Inger Vedin
    Department of Medicine and the Division of Clinical Nutrition and Metabolism Research, Uppsala University Hospital, Uppsala, Sweden
    Am J Clin Nutr 87:1616-22. 2008
    ..Whereas most studies have explored the effects of predominantly EPA-based n-3 FAs preparations, few have addressed the effects of n-3 FAs preparations with DHA as the main FA...
  31. ncbi High replication fitness and transmission efficiency of HIV-1 subtype C from India: Implications for subtype C predominance
    Milka A Rodriguez
    Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, 426 Parran Hall, GSPH, 130 DeSoto Street, Pittsburgh, PA 15261, USA
    Virology 385:416-24. 2009
    ..These data provide a model to explain the asymmetric distribution of subtype C over other subtypes in India...
  32. ncbi Gene expression analysis in blood cells in response to unmodified and 2'-modified siRNAs reveals TLR-dependent and independent effects
    Lina Cekaite
    Department of Tumour Biology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Montebello, 310, Oslo, Norway
    J Mol Biol 365:90-108. 2007
    ..The data should help to define strategies to either enhance or avoid the non-specific effects of siRNAs in order to develop safe therapeutics...
  33. pmc Construction and analysis of an infectious human Immunodeficiency virus type 1 subtype C molecular clone
    B Renjifo
    Harvard AIDS Institute and Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Virol 75:4964-72. 2001
    ..The clone may also be used in experimental design of vaccine candidates that may be directed against HIV-1 subtype C...
  34. ncbi Enhanced rates and magnitude of immune responses detected against an HIV vaccine: effect of using an optimized process for isolating PBMC
    Lisa S Kierstead
    Vaccine and Biologics Research, Merck Research Laboratories, West Point and Wayne, PA 19087, USA
    AIDS Res Hum Retroviruses 23:86-92. 2007
    ..These studies establish the importance of optimal conditions for PBMC collection and handling to the success of a clinical development program...
  35. ncbi Brain dendritic cells and macrophages/microglia in central nervous system inflammation
    H G Fischer
    Institute for Medical Microbiology and Virology, Heinrich Heine University, Duesseldorf, Germany
    J Immunol 166:2717-26. 2001
    ..These DC functionally mature upon disease onset and can differentiate from resident microglia. Their emergence, maturation, and prolonged activity within the brain might contribute to the chronicity of intracerebral Th1 responses...
  36. ncbi Lectin-like oxidized LDL receptor-1 (LOX-1) supports adhesion of mononuclear leukocytes and a monocyte-like cell line THP-1 cells under static and flow conditions
    Kazutaka Hayashida
    Department of Geriatric Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    FEBS Lett 511:133-8. 2002
    Adhesion of mononuclear leukocytes to vascular endothelial cells appears one of the initial steps in the process of atherogenesis and inflammation...
  37. pmc EphA2 as a glioma-associated antigen: a novel target for glioma vaccines
    Manabu Hatano
    Department of Neurological Surgery, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
    Neoplasia 7:717-22. 2005
    ..Taken together, these data suggest that EphA2883-891 may be an attractive antigen epitope for molecularly targeted glioma vaccines...
  38. ncbi Levels of the potential biomarker p11 in peripheral blood cells distinguish patients with PTSD from those with other major psychiatric disorders
    Tung Ping Su
    Division of Psychiatry, Faculty of Medicine, National Yang Ming University, Taipei, Taiwan
    J Psychiatr Res 43:1078-85. 2009
    ..Our findings suggest that PBMC p11 mRNA expression levels may serve as a potential biomarker to distinguish PTSD from BP, MDD and SCZ...
  39. ncbi High-dose leptin activates human leukocytes via receptor expression on monocytes
    H Zarkesh-Esfahani
    Division of Clinical Sciences (North, University of Sheffield, Sheffield, United Kingdom
    J Immunol 167:4593-9. 2001
    ..This is of relevance when considering leptin therapy and may partly explain the relationship among leptin, proinflammatory cytokines, insulin resistance, and obesity...
  40. ncbi Acute rejection of experimental lung allografts: characterization of intravascular mononuclear leukocytes
    Sonja Blöcher
    Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Justus Liebig University Giessen, University of Giessen Lung Center, Giessen, Germany
    Clin Immunol 124:98-108. 2007
    ..In conclusion, during rejection, monocytes with unusual properties accumulate and T-lymphocytes are activated in lung allograft blood vessels...
  41. pmc Cytokine gene expression in peripheral blood mononuclear cells and tissues of cattle infected with Mycobacterium avium subsp. paratuberculosis: evidence for an inherent proinflammatory gene expression pattern
    Paul M Coussens
    Department of Animal Science and Center for Animal Functional Genomics, Michigan State University, East Lansing Michigan 48824, USA
    Infect Immun 72:1409-22. 2004
    ..avium subsp. paratuberculosis develop in infected cattle and that a likely place for development and expansion of these cell populations is the mesenteric lymph nodes draining sites of infection...
  42. ncbi Therapeutic potential of ex vivo expanded endothelial progenitor cells for myocardial ischemia
    A Kawamoto
    Division of Cardiovascular Research, St Elizabeth's Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA
    Circulation 103:634-7. 2001
    ..CONCLUSIONS: Ex vivo expanded EPCs incorporate into foci of myocardial neovascularization and have a favorable impact on the preservation of left ventricular function...
  43. ncbi Suppression of microRNA-silencing pathway by HIV-1 during virus replication
    Robinson Triboulet
    Laboratoire de Virologie Moleculaire, Institut de Genetique Humaine, Montpellier, France
    Science 315:1579-82. 2007
    ..Our results highlight the involvement of the miRNA-silencing pathway in HIV-1 replication and latency...
  44. doi Evaluating neutralizing antibodies against HIV, SIV, and SHIV in luciferase reporter gene assays
    David C Montefiori
    Duke University Medical Center, Durham, North Carolina, USA
    Curr Protoc Immunol . 2005
    ..Both assays are performed in a 96-well format and use tat-responsive luciferase reporter gene expression as readout...
  45. ncbi Bovine and ovine blood mononuclear leukocytes differ markedly in innate immune responses induced by Class A and Class B CpG-oligodeoxynucleotide
    Angelo Mena
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK Canada S7N 5E3
    Oligonucleotides 13:245-59. 2003
    ..A striking aspect of these results is that such marked differences in CpG-ODN induced innate responses existed both between and within two closely related species...
  46. ncbi Upregulation of interleukin-10 gene expression in the leukocytes of pigs infected with porcine reproductive and respiratory syndrome virus
    Sanipa Suradhat
    The Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Pathumwan, Bangkok 10330, Thailand
    J Gen Virol 84:2755-60. 2003
    ..Our data support the notion that induction of IL-10 production may be one of the strategies used by PRRSV to modulate the host's immune responses, and this may contribute to the unique clinical picture observed following PRRSV infection...
  47. pmc Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation
    Luigi Buonaguro
    Lab, Viral Oncogenesis and Immunotherapies and AIDS Reference Center, Department of Experimental Oncology, Istituto Nazionale Tumori Fond, G, Pascale, 80131 Napoli, Italy
    BMC Bioinformatics 9:S5. 2008
    ..Baculovirus-expressed HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs) induce maturation and activation of monocyte-derived dendritic cells (MDDCs) with a production of Th1- and Th2-specific cytokines...
  48. pmc Identification of candidate genes in scleroderma-related pulmonary arterial hypertension
    Dmitry N Grigoryev
    Division of Allergy and Clinical Immunology, Johns Hopkins University, Baltimore, MD 21224, USA
    Transl Res 151:197-207. 2008
    ..Deciphering the role of genes involved in vascular remodeling and PAH development may reveal new treatment targets for this devastating disorder...
  49. ncbi The association between microsatellite polymorphisms in intron II of the human Toll-like receptor 2 gene and tuberculosis among Koreans
    J J Yim
    Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, and Lung Institute, Seoul National University College of Medicine, Chongno Gu, Seoul, Republic of Korea
    Genes Immun 7:150-5. 2006
    ..This association is correlated with lower expression of TLR2 through weaker promoter activity for genes with shorter GT repeats...
  50. ncbi Residual cell-associated unspliced HIV-1 RNA in peripheral blood of patients on potent antiretroviral therapy represents intracellular transcripts
    Marek Fischer
    Department of Medicine, University Hospital Zurich, Switzerland
    Antivir Ther 7:91-103. 2002
    ..As shown by limiting dilution analysis, HIV-1 infected cells with such a repressed viral transcription pattern were observed at high frequencies in PBMC from untreated patients...
  51. ncbi In vivo transcription of bovine leukemia virus and bovine immunodeficiency-like virus
    Donglai Wu
    National Institute of Animal Health, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
    Virus Res 97:81-7. 2003
    ..These results indicated that BLV can infect T cells and monocytes as well as B cells, but can be expressed by transcription only in B cells. In contrast, BIV can express its transcripts in all infected cells...
  52. ncbi Low expression of interferon-stimulated genes in active multiple sclerosis is linked to subnormal phosphorylation of STAT1
    Xuan Feng
    Department of Neurology, MC 2030, University of Chicago, 5841 South Maryland Ave, Chicago, IL 60637, USA
    J Neuroimmunol 129:205-15. 2002
    ..Excessive P-Tyr-STAT1 responses could induce inflammatory cytokines and demyelination in MS, as in motheaten mice, which have defects in SHP-1 function. Abnormal IFN signaling may predict the course of MS and responses to therapy...
  53. pmc Evolution of a TRIM5-CypA splice isoform in old world monkeys
    Ruchi M Newman
    Department of Microbiology and Molecular Genetics, New England Primate Research Center, Harvard Medical School, Southborough, Massachusetts, United States of America
    PLoS Pathog 4:e1000003. 2008
    ..Whether the formation of novel genes through alternative splicing has played a wider role in the evolution of the TRIM family remains to be investigated...
  54. ncbi Development of a DNA vaccine designed to induce cytotoxic T lymphocyte responses to multiple conserved epitopes in HIV-1
    Cara C Wilson
    University of Colorado Health Sciences Center, Denver, CO 80262, USA
    J Immunol 171:5611-23. 2003
    ..These data indicate that the EP HIV-1090 DNA vaccine may be suitable for inducing relevant HIV-1-specific CTL responses in humans...
  55. ncbi Gene microarray analysis of peripheral blood cells in pulmonary arterial hypertension
    Todd M Bull
    Division of Pulmonary Sciences and Critical Care Medicine, 4200 East 9th Avenue, Denver, CO 80262, USA
    Am J Respir Crit Care Med 170:911-9. 2004
    ..Microarray expression profiling of peripheral blood cells can discriminate between patients with PAH and normal volunteers. These findings may have important implications toward diagnosis, screening, and pathogenesis of this disease...
  56. ncbi Primed peripheral polymorphonuclear leukocyte: a culprit underlying chronic low-grade inflammation and systemic oxidative stress in chronic kidney disease
    Shifra Sela
    Eliachar Research Laboratory, Western Galilee Hospital, Nahariya 22100, Israel
    J Am Soc Nephrol 16:2431-8. 2005
    ..Hence, PMNL priming is a key mediator of low-grade inflammation and OS associated with renal failure, occurring before the onset of RRT and further augmented in chronic HD...
  57. ncbi Isolation and expression profiling of genes upregulated in bone marrow-derived mononuclear cells of rheumatoid arthritis patients
    Nobuo Nakamura
    Center of Arthroplasty, Kyowakai Hospital, Suita, Japan
    DNA Res 13:169-83. 2006
    ..We propose that the expression profiling of these AURA genes may improve our understanding of the pathogenesis of RA...
  58. ncbi Mitogen-induced upregulation of hepatitis C virus expression in human lymphoid cells
    Tram N Q Pham
    Molecular Virology and Hepatology Research, Division of Basic Medical Science, Faculty of Medicine, Health Sciences Centre, Memorial University, St John s, Newfoundland, Canada A1B 3V6
    J Gen Virol 86:657-66. 2005
    ..As such, this method offers a new investigative tool to study HCV lymphotropism and to monitor virus presence during the course of HCV infection...
  59. ncbi Cytokine expression during the outcome of canine experimental infection by Leishmania infantum
    Gabriela M Santos-Gomes
    Unidade de Leishmanioses, Centro de Malaria e Outras Doencas Tropicais, Instituto de Higiene e Medicina Tropical IHMT, Universidade Nova de Lisboa, Rua da Junqueira 96, 1349 008 Lisbon, Portugal
    Vet Immunol Immunopathol 88:21-30. 2002
    ..The predominance of a Th1 type response for a relatively short period indicates that these cytokines are required to control the infection delaying the appearance of progressive disease...
  60. ncbi Differential and competitive activation of human immune cells by distinct classes of CpG oligodeoxynucleotide
    Mayda Gursel
    Section of Retroviral Research, Center for Biologics Evaluation and Research, Food and Drug Administration, Bldg 29A Rm 3D10, 8800 Rockville Pike, Bethesda, MD 20892, USA
    J Leukoc Biol 71:813-20. 2002
    ..Thus, different types of CpG ODN have distinct and in some cases incompatible effects on the same cells, a finding with important implications for the therapeutic use of these agents...
  61. ncbi Early levels of HIV-1 DNA in peripheral blood mononuclear cells are predictive of disease progression independently of HIV-1 RNA levels and CD4+ T cell counts
    Christine Rouzioux
    Laboratoire de Virologie, Paris, France
    J Infect Dis 192:46-55. 2005
    ....
  62. ncbi Marked MMP-2 transcriptional up-regulation in mononuclear leukocytes invading the subarachnoidal space in aseptic suppurative steroid-responsive meningitis-arteritis in dogs
    M Schwartz
    Department of Small Animal Medicine and Surgery, School of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany
    Vet Immunol Immunopathol 133:198-206. 2010
    ....
  63. ncbi Inhibition of HIV strains by GB virus C in cell culture can be mediated by CD4 and CD8 T-lymphocyte derived soluble factors
    Susan Jung
    Institute of Clinical and Molecular Virology, Friedrich Alexander Universitat Erlangen Nurnberg, Germany
    AIDS 19:1267-72. 2005
    ..To address the possibility of direct inhibitory mechanisms, we studied the impact of GBV-C on HIV-1 replication in vitro...
  64. ncbi Measurement of cytokine release at the single cell level using the ELISPOT assay
    Josephine H Cox
    U S Military HIV Research Program, Henry M Jackson Foundation for the Advancement of Military Medicine, 13 Taft Court, Rockville, MD 20850, USA
    Methods 38:274-82. 2006
    ..The history, applications, validation process, and future challenges of the ELISPOT assay are discussed in this chapter...
  65. ncbi Effect of heterosexual intercourse on mucosal alloimmunisation and resistance to HIV-1 infection
    Barry Peters
    Department of Genitourinary Medicine Guy s, King s and St Thomas Hospital Medical and Dental School, London SE1 9RT, UK
    Lancet 363:518-24. 2004
    ..We investigated this hypothesis in people practising unprotected sex and those using protection...
  66. ncbi Evaluation of bioavailability of three types of IFNbeta in multiple sclerosis patients by a new quantitative-competitive-PCR method for MxA quantification
    A Bertolotto
    Centro Sclerosi Multipla and Laboratorio di Neurobiologia Clinica, Divisione Universitaria di Neurologia, Azienda Ospedaliera S Luigi, Universita di Torino, Regione Gonzole 10, 10043 Orbassano, Italy
    J Immunol Methods 256:141-52. 2001
    ..Our data indicate that the bioavailability of the three available types of IFNbeta can be evaluated by MxA qc-PCR...
  67. ncbi High frequency of X4/DM-tropic viruses in PBMC samples from patients with primary HIV-1 subtype-B infection in 1996-2007: the French ANRS CO06 PRIMO Cohort Study
    Pierre Frange
    EA 3620, AP HP, Laboratoire de Virologie, Hopital Necker Enfants Malades, Universite Paris Descartes, Paris, France
    J Antimicrob Chemother 64:135-41. 2009
    ....
  68. ncbi CpG-A-induced monocyte IFN-gamma-inducible protein-10 production is regulated by plasmacytoid dendritic cell-derived IFN-alpha
    Sue E Blackwell
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 170:4061-8. 2003
    ..These studies provide a better understanding of the mechanisms through which CpG ODN create a Th1-like environment...
  69. ncbi A novel source of viable peripheral blood mononuclear cells from leukoreduction system chambers
    Allan B Dietz
    Division of Transfusion Medicine, Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, Minnesota 55095, USA
    Transfusion 46:2083-9. 2006
    ..Buffy coats are becoming less available as a source of research-grade peripheral blood mononuclear cells (PBMNCs). Therefore, alternative sources of these cells were investigated...
  70. pmc Reduced CD4 T cell activation and in vitro susceptibility to HIV-1 infection in exposed uninfected Central Africans
    Evelyne Begaud
    Institut Pasteur, Bangui, CAR
    Retrovirology 3:35. 2006
    ....
  71. ncbi Construction and in vitro properties of SIVmac mutants with deletions in "nonessential" genes
    J S Gibbs
    Harvard Medical School, New England Regional Primate Research Center, Southborough, Massachusetts 01772 9102
    AIDS Res Hum Retroviruses 10:607-16. 1994
    ....
  72. ncbi Transplantation of endothelial progenitor cells for therapeutic neovascularization
    Atsuhiko Kawamoto
    Division of Cardiovascular Research, St Elizabeth s Medical Center, Tufts University School of Medicine, 736 Cambridge Street, Boston, MA, USA
    Cardiovasc Radiat Med 3:221-5. 2002
    ..Further basic research, with improved understanding of the mechanisms governing homing and incorporation of EPCs, will be still necessary to optimize the methodology of the cell therapy...
  73. pmc Prevalence, clinical relevance and characterization of circulating cytotoxic CD4+CD28- T cells in ankylosing spondylitis
    Christina Duftner
    Department of Internal Medicine, University of Innsbruck, Innsbruck, Austria
    Arthritis Res Ther 5:R292-300. 2003
    ..MHC class I recognizing and activating natural killer cell receptors on the surface of CD4+CD28- T cells may be involved in a HLA-B27 mediated co-stimulation of these proinflammatory and cytotoxic cells...
  74. ncbi Human immunodeficiency virus type 1 (HIV-1) circulating recombinant form 02_AG (CRF02_AG) has a higher in vitro replicative capacity than its parental subtypes A and G
    Frank A J Konings
    Department of Microbiology, New York University School of Medicine, New York, New York 10010, USA
    J Med Virol 78:523-34. 2006
    ..This adaptation could contribute to the broader spread of HIV-1 CRF02_AG leading to its predominance in West Central Africa compared to the lower prevalence of its parental subtypes A and G...
  75. ncbi Nef-mediated suppression of T cell activation was lost in a lentiviral lineage that gave rise to HIV-1
    Michael Schindler
    Department of Virology, University of Ulm, 89081 Ulm, Germany
    Cell 125:1055-67. 2006
    ..This function was lost during viral evolution in a lineage that gave rise to HIV-1 and may have predisposed the simian precursor of HIV-1 for greater pathogenicity in humans...
  76. ncbi Fasting induces changes in peripheral blood mononuclear cell gene expression profiles related to increases in fatty acid beta-oxidation: functional role of peroxisome proliferator activated receptor alpha in human peripheral blood mononuclear cells
    Mark Bouwens
    Nutrition, Metabolism, and Genomics Group, Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
    Am J Clin Nutr 86:1515-23. 2007
    ..PBMCs express PPARalpha, making these cells interesting to study FA-dependent gene expression...
  77. pmc Establishing acceptance criteria for cell-mediated-immunity assays using frozen peripheral blood mononuclear cells stored under optimal and suboptimal conditions
    Jeffrey G Smith
    Vaccine and Biologics Research, Merck Research Laboratories, WP26B 1144, Merck and Co, Inc, 770 Sumneytown Pike, West Point, PA 19486, USA
    Clin Vaccine Immunol 14:527-37. 2007
    ..Although all three AC measures could be effectively applied, the apoptosis AC limit applied was best for separating samples that could respond to antigenic stimulation from samples that could not effectively respond...
  78. ncbi A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients
    S Farzana Hussain
    Department of Neurosurgery, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Cancer Res 67:9630-6. 2007
    ....
  79. ncbi Non-canonical Wnt signaling enhances differentiation of human circulating progenitor cells to cardiomyogenic cells
    Masamichi Koyanagi
    Department of Molecular Cardiology, Internal Medicine IV and Institute of Cardiovascular Physiology, University of Frankfurt, Theodor Stern Kai 7, 60590 Frankfurt, Germany
    J Biol Chem 280:16838-42. 2005
    ..Our data demonstrate that Wnt11, but not Wnt3A, augments cardiomyogenic differentiation of human CPCs. Wnt11 promotes cardiac differentiation via the non-canonical PKC-dependent signaling pathway...
  80. pmc Envelope determinants of equine infectious anemia virus vaccine protection and the effects of sequence variation on immune recognition
    Tara L Tagmyer
    E1240 Biomedical Science Tower, Department of Microbiology and Molecular Genetics, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Virol 82:4052-63. 2008
    ....
  81. ncbi Gastrin induces the interaction between human mononuclear leukocytes and endothelial cells through the endothelial expression of P-selectin and VCAM-1
    Sales Ibiza
    Departamento de Farmacología and CIBERehd, Universidad de Valencia, Spain
    Am J Physiol Cell Physiol 297:C1588-95. 2009
    ..Gastrin did not affect the interactions between HUVEC and PMN. Gastrin induces interactions between human mononuclear leukocytes and endothelial cells through the activation of CCK-2 receptors and the enhancement of endothelial P-..
  82. ncbi Multiple sclerosis retrovirus particles and recombinant envelope trigger an abnormal immune response in vitro, by inducing polyclonal Vbeta16 T-lymphocyte activation
    H Perron
    bioMérieux Pierre Fabre, R and D, Chemin de l orme, Marcy l Etoile, 69280, France
    Virology 287:321-32. 2001
    ..Molecular analysis of Vbeta CDR3 showed that Vbeta16 expansions are polyclonal. Our results thus provide evidence that MSRV envelope protein can trigger an abnormal immune response with similar characteristics to that of superantigens...
  83. ncbi Brains and peripheral blood mononuclear cells of multiple sclerosis (MS) patients hyperexpress MS-associated retrovirus/HERV-W endogenous retrovirus, but not Human herpesvirus 6
    Giuseppe Mameli
    Section of Microbiology, Department of Biomedical Sciences, Center of Excellence for Biotechnology Development and Biodiversity Research, University of Sassari, Sassari, Italy
    J Gen Virol 88:264-74. 2007
    ....
  84. ncbi A phase I study of interleukin 12 with trastuzumab in patients with human epidermal growth factor receptor-2-overexpressing malignancies: analysis of sustained interferon gamma production in a subset of patients
    Robin Parihar
    Department of Molecular Virology, Immunology, and Medical Genetics, The Arthur G James Comprehensive Cancer Center and Solove Research Institute, The Ohio State University, Columbus, Ohio 43210, USA
    Clin Cancer Res 10:5027-37. 2004
    ..The IL-12 component was dose-escalated within cohorts of 3 patients (30, 100, 300, or 500 ng/kg). Correlative assays were conducted using serum samples and peripheral blood cells obtained during the course of therapy...
  85. ncbi Comparative analysis of methods for assessment of circulating endothelial progenitor cells
    Jacob George
    The Department of Cardiology, Tel Aviv University, Israel
    Tissue Eng 12:331-5. 2006
    ..These findings may suggest that CD34/KDR is more appropriate for the definition of circulating EPC, whereas CFU numbers are more likely to reflect their ability to proliferate...
  86. ncbi Residual HIV-RNA levels persist for up to 2.5 years in peripheral blood mononuclear cells of patients on potent antiretroviral therapy
    M Fischer
    Department of Internal Medicine, University Hospital Zurich, Switzerland
    AIDS Res Hum Retroviruses 16:1135-40. 2000
    ..69; p = 0.003). These findings support the concept that measurement of HIV-1 RNA in the easily accessible PBMC compartment is relevant for evaluating the potency of current and future antiretroviral therapies...
  87. ncbi Expression of toll-like receptors 2 and 4 in rheumatoid synovial tissue and regulation by proinflammatory cytokines interleukin-12 and interleukin-18 via interferon-gamma
    Timothy R D J Radstake
    University Medical Center Nijmegen, Nijmegen, The Netherlands
    Arthritis Rheum 50:3856-65. 2004
    ..To study the expression of Toll-like receptor 2 (TLR-2) and TLR-4 and its association with proinflammatory cytokines in synovial tissue from patients with rheumatoid arthritis (RA), osteoarthritis (OA), and healthy individuals...
  88. ncbi Functional requirement for SAP in 2B4-mediated activation of human natural killer cells as revealed by the X-linked lymphoproliferative syndrome
    S G Tangye
    Centenary Institute for Cancer Medicine and Cell Biology, and University of Sydney, New South Wales, Australia
    J Immunol 165:2932-6. 2000
    ..Despite this, CD2 or CD16-induced cytotoxicity of SAP-deficient NK cells was similar to that of normal NK cells. Thus, selective impairment of 2B4-mediated NK cell activation may contribute to the immunopathology of XLP...
  89. pmc HIV infection of naturally occurring and genetically reprogrammed human regulatory T-cells
    Kyra Oswald-Richter
    Department of Microbiology and Immunology, Vanderbilt University Medical School, Nashville, Tennessee, USA
    PLoS Biol 2:E198. 2004
    ..Moreover, the ability to reprogram human T-cells into Treg cells in vitro will greatly aid in decoding their mechanism of suppression, their enhanced susceptibility to HIV infection, and the unique markers expressed by this subset...
  90. pmc The replicative fitness of primary human immunodeficiency virus type 1 (HIV-1) group M, HIV-1 group O, and HIV-2 isolates
    Kevin K Ariën
    Centre for AIDS Research, Case Western Reserve University, Cleveland, OH, USA
    J Virol 79:8979-90. 2005
    ..These results suggest that reduced replicative and transmission fitness may be contributing to the low prevalence and limited geographical spread of HIV-2 and group O HIV-1 in the human population...
  91. ncbi The combination of apoptotic U937 cells and lupus IgG is a potent IFN-alpha inducer
    U Båve
    Section of Rheumatology, Department of Medical Sciences, Uppsala University, Sweden
    J Immunol 165:3519-26. 2000
    ..This mechanism for induction of IFN-alpha production could well be operative also in vivo, explain the IFN-alpha production seen in SLE patients, and be important in the pathogenesis of SLE...
  92. pmc Reduced prostaglandin F2 alpha release from blood mononuclear leukocytes after oral supplementation of omega3 fatty acids: the OmegAD study
    Inger Vedin
    Department of Medicine, Caring Sciences and Society, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    J Lipid Res 51:1179-85. 2010
    ..The stimulus-specific PGF(2alpha) release from PBMC after 6 months of oral supplementation with the DHA-rich fish oil might be one event related to reduced inflammatory reactions associated with omega-3 fatty acid intake...
  93. pmc Immune complexes from SLE sera induce IL10 production from normal peripheral blood mononuclear cells by an FcgammaRII dependent mechanism: implications for a possible vicious cycle maintaining B cell hyperactivity in SLE
    J Ronnelid
    Department of Clinical Immunology, Rudbeck Laboratory, University Hospital, Uppsala University, S 751 85 Uppsala, Sweden
    Ann Rheum Dis 62:37-42. 2003
    ..These immune complexes can then stimulate cellular reactions through Fc and complement receptors...
  94. pmc Lymphocyte apoptosis during classical swine fever: implication of activation-induced cell death
    A Summerfield
    Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    J Virol 72:1853-61. 1998
    ..Taken together, these results imply that activation-induced programmed cell death was the mechanism behind lymphopenia during classical swine fever...
  95. pmc HIV-1 escape from a small molecule, CCR5-specific entry inhibitor does not involve CXCR4 use
    Alexandra Trkola
    Division of Infectious Diseases, Department of Medicine, University Hospital Zurich, 8091 Zurich, Switzerland
    Proc Natl Acad Sci U S A 99:395-400. 2002
    ..Instead, HIV-1 acquires the ability to use CCR5 despite the inhibitor, first by requiring lower levels of CCR5 for entry and then probably by using the drug-bound form of the receptor...
  96. pmc Reverse transcriptase inhibitors as potential colorectal microbicides
    Carolina Herrera
    Division of Cellular and Molecular Medicine, St George s University of London, Cranmer Terrace, London SW17 0RE, United Kingdom
    Antimicrob Agents Chemother 53:1797-807. 2009
    ..These findings may have important implications for the rational design of effective rectal microbicides...
  97. ncbi Butter and walnuts, but not olive oil, elicit postprandial activation of nuclear transcription factor kappaB in peripheral blood mononuclear cells from healthy men
    Cecilia Bellido
    Lipids and Arteriosclerosis Unit, Hospital Universitario Reina Sofia, Cordoba, Spain
    Am J Clin Nutr 80:1487-91. 2004
    ....
  98. pmc Cell-to-cell contact results in a selective translocation of maternal human immunodeficiency virus type 1 quasispecies across a trophoblastic barrier by both transcytosis and infection
    S Lagaye
    Institut Pasteur, Unité de Biologie des Rétrovirus, 75 724 Paris Cedex 15, France
    J Virol 75:4780-91. 2001
    ....
  99. ncbi Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo
    Marek Fischer
    Division of Infectious Diseases and Hospital Epidemiology, Department of Medicine, University Hospital Zurich, 8091 Zurich, Switzerland
    Antivir Ther 8:97-104. 2003
    ..In contrast, expression of HIV-UsRNAPBMC increased during STI and consequently provides a more sensitive, albeit not absolute cellular marker of ongoing HIV-1 replication...
  100. ncbi Noninvasive discrimination of rejection in cardiac allograft recipients using gene expression profiling
    M C Deng
    Columbia University, New York, NY, USA
    Am J Transplant 6:150-60. 2006
    ..Additional clinical experience is needed to establish the role of molecular testing for clinical event prediction and immunosuppression management...
  101. pmc Viability and functional activity of cryopreserved mononuclear cells
    A Weinberg
    University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Clin Diagn Lab Immunol 7:714-6. 2000
    ..Preparations with viability greater than or equal to 70% had consistent proliferative responses and were suitable for functional analyses...

Research Grants62

  1. PATHOPHYSIOLOGY OF THE ENDOTHELIUM
    Francis W Luscinskas; Fiscal Year: 2013
    ..CD47 dependent regulation of VLA-4 and LFA-1 integrin adhesion, in the recruitment of inflammatory mononuclear leukocytes into sites of chronic inflammation...
  2. ROLE OF MONONUCLEAR LEUKOCYTES IN IMMUNITY
    Samuel Silverstein; Fiscal Year: 2009
    ..In other words, must cytotoxic lymphocytes reach a critical concentration within a tumor bed to effect a reduction in tumor mass? ..
  3. Vascular adhesion protein-1 (VAP-1) as a therapeutic target in stroke
    DALE ALAN PELLIGRINO; Fiscal Year: 2013
    ..e., mononuclear leukocytes) in the neuropathology accompanying temporary MCAo...
  4. Alpha M Beta 2 integrin blockade for acute inflammatory neuropathies
    EROBOGHENE EKAMERENO UBOGU; Fiscal Year: 2013
    ..and ICAM-1 expressed on activated endoneurial endothelial cells interact and recruit pathogenic mononuclear leukocytes across the blood-nerve barrier in AIDP...
  5. Mechanisms of risk for sulfoniamide hypersensitivity
    Lauren A Trepanier; Fiscal Year: 2013
    ..Specific aim 1b will focus on the observation that peripheral blood mononuclear leukocytes (PBMCs) from HS patients are more susceptible to toxicity from sulfonamide metabolites compared to drug ..
  6. Fibronectin peptide antagonism for chronic inflammatory neuropathies
    EROBOGHENE EKAMERENO UBOGU; Fiscal Year: 2012
    ..hypothesis: Endothelial FN CS1 actively participates in the recruitment of pathogenic hematogenous mononuclear leukocytes across the blood-nerve barrier in CIDP...
  7. alpha4beta1 Intergrin Signaling In Immunity And Arthritis
    GREGG JOSHUA SILVERMAN; Fiscal Year: 2010
    ..We will also test the hypothesis that the a4-paxillin interaction is required for effective trafficking of mononuclear leukocytes to sites of inflammation...
  8. MONONUCLEAR LEUKOCYTES AND IMMUNITY
    Samuel Silverstein; Fiscal Year: 2003
    ..We are exploring the mechanisms that control cell migration, and adhesion; and the effects of different matrix proteins on these processes. ..
  9. DIETARY LIPIDS--EFFECTS ON LIPID-LIPOPROTEIN METABOLISM
    D Illingworth; Fiscal Year: 1991
    ..assessed by serum and urinary excretion of mevalonic acid and cholesterol synthesis in freshly isolated mononuclear leukocytes. These four diets will be fed to subjects consuming a cholesterol-free diet and in the presence of 600 mg/..
  10. Carbon monoxide therapy for severe pulmonary arterial hypertension
    Roberto F Machado; Fiscal Year: 2013
    ..attenuation of pulmonary vascular remodeling;ii) a molecular signature can be defined in peripheral blood mononuclear leukocytes (PBMCs) which predict CO responsiveness...
  11. RENAL CELL CARCINOMA--ADOPTIVE IMMUNOTHERAPY
    KURT STENZEL; Fiscal Year: 1990
    ..successful immunotherapy for human renal cell carcinoma based on the adoptive transfer of autologous, mononuclear leukocytes (PBM) activated in a novel way (combined treatment with sodium periodate (IO-4) and recombinant IL2 (rIL2))..
  12. HYALURONAN-LEUKOCYTE INTERACTIONS IN ASTHMATIC AIRWAYS
    Vincent Hascall; Fiscal Year: 2006
    ..a unique hyaluronan network, synthesized in response to the viral mimic, poly I:C, specifically engages mononuclear leukocytes via a process involving the hyaluronan binding protein, CD44, on the leukocyte surface...
  13. VCAM-1 AND CHEMOKINES AND MONOCYTE RECRUITMENT
    Anthony Rosenzweig; Fiscal Year: 2000
    ..expression of adhesion molecules and cytokines significantly modulates the adherence and infiltration of mononuclear leukocytes in the vessel wall, and that these leukocytes, in turn, influence atherogenesis...
  14. PHOSPHODIESTERASE STUDIES IN ATOPIC DERMATITIS
    Jon Hanifin; Fiscal Year: 1990
    ..Another major effort will be directed at induction of leukocyte activation markers and abnormal PDE mononuclear leukocytes by immune effector factors produced by AD leukocyte cultures and potentially by specific cytokines...
  15. EXTRAVASCULAR THROMBIN REGULATION BY HEPARIN COFACTOR II
    FRANK CHURCH; Fiscal Year: 2004
    ..domain; and three, characterize the ability of HCII to regulate thrombin's cytokine-like activity for mononuclear leukocytes and endothelial cells exposed to pro- and antiatherogenic substances, and to study the in vivo ..
  16. IMMUNOSUPPRESSION BY AN INHIBITOR OF INTERLEUKIN 2
    MARTHA CATHCART; Fiscal Year: 1991
    ..HSF mediates the suppression of antibody production by normal human peripheral blood mononuclear leukocytes and also inhibits interleukin 2 (IL2) production by T lymphocytes...
  17. INTERACTIONS OF ENVIRONMENTAL TOXICANTS WITH LEUKOCYTES
    Michael Trush; Fiscal Year: 2002
    DESCRIPTION: (Adapted from the Investigator's Abstract)Polymorphonuclear (PMN's) and mononuclear leukocytes are important cells in host defense mechanisms...
  18. TRANSENDOTHELIAL MIGRATION MONONUCLEAR LEUKOCYTES HIV1
    Holly Birdsall; Fiscal Year: 2000
    ..goal of this application is to identify mechanisms whereby HIV-1 infection influences the migration of mononuclear leukocytes (MNLs) across vascular endothelial barrier and the accumulation of infected cells in tissues outside of ..
  19. ORIGINS OF CANCER SUSCEPTIBILITY--CELLS VS PLASMA
    GEORGE ROUSH; Fiscal Year: 1993
    ..transferase (ADPRT) and unscheduled DNA synthesis (UDS) are inter-related measures that may be obtained in mononuclear leukocytes from venous blood. They reflect DNA repair, and are affected by pro-oxidant states...
  20. IMMUNOGLOBULIN GENES AS MARKERS FOR RESIDUAL ALL
    Richard Smith; Fiscal Year: 1991
    ..have been prepared and assays constructed which detect 10 leukemic cells in a background of 10 6 normal mononuclear leukocytes. Using these methods, we now propose to develop a quantitative assay for leukemia-specific HVR3 alleles...
  21. EFFECTS OF IMMOBILIZED LIGANDS ON MONONUCLEAR LEUKOCYTES
    Josef Michl; Fiscal Year: 1980
    ..The availibility of the cytoplasmic side of this membrane also allows us to analyze by morphological means the relationship between cytoskeleton and adherent plasma membrane under the various modulating conditions...
  22. Endothelial Regulation of Leukocyte Transmigration
    FRANCIS LUSCINSKAS; Fiscal Year: 2009
    The interaction of neutrophils and mononuclear leukocytes with endothelium plays a pivotal role in leukocyte recruitment in acute and chronic inflammation, immune reactions, vascular injury and atherosclerosis...
  23. TUMOR CYTOLYSIS BY MONONUCLEAR LEUKOCYTES
    Dorothea Zucker Franklin; Fiscal Year: 1990
    Studies will be designed to elucidate how unsensitized mononuclear leukocytes obtained from the peripheral blood of healthy individuals recognize and kill tumor cells...
  24. ROLE OF HYALURONAN IN INFLAMMATORY BOWEL DISEASE
    Scott Strong; Fiscal Year: 2005
    ..whereby virus-infected/viral analog-induced M-SMCs elaborate hyaluronan that engages CD44 expressed by mononuclear leukocytes. We hypothesize that hyaluronan, produced by virus- infected M-SMCs, results in recruitment of leukocytes ..
  25. ENDOTHELIAL CELL DYSFUNCTION IN CHRONIC IMMUNE DISEASES
    FRANCIS LUSCINSKAS; Fiscal Year: 2003
    ..diseases, a characteristic feature is the continued recruitment and accumulation in tissues of mononuclear leukocytes (lymphocytes and monocytes)...
  26. FUNCTION AND REGULATION OF IL 10 IN LUNG CANCER
    Steven Dubinett; Fiscal Year: 2001
    ..While the interaction between lung tumor cells and mononuclear leukocytes is complex, one focus of our in vitro studies will be to identify the mechanism(s) by which human NSCLC ..
  27. IMMUNE-ADRENAL AXIS IN AIDS
    James Melby; Fiscal Year: 1991
    ..In parallel with these studies, mononuclear leukocytes will be harvested to assess their production or cytokines which can stimulate the HPA axis (adrenotropic) ..
  28. STRUCTURE AND FUNCTION OF HUMAN CD8 ALPHA AND CD8 BETA
    Neal Flomenberg; Fiscal Year: 1992
    ..To determine the predominant CD8 Beta isoform expressed by human peripheral mononuclear leukocytes and thymocytes. To identify the requirements for human CD8 beta surface expression...
  29. STRESS AND CNS INFECTION OF AN ALPHA HERPESVIRUS
    Stefanie Rassnick; Fiscal Year: 1999
    ..The effects of stress on the responses of glial cells and mononuclear leukocytes to the viral infection will be examined in relation to the passage and cytopathogenicity of PRV in the CNS ..
  30. ROLE OF MONONUCLEAR LEUKOCYTES IN GRAFT ARTERIOSCLEROSIS
    Anthony Rosenzweig; Fiscal Year: 2001
    ..in transplanted aorta in vivo the molecules that effectively and specifically supported adhesion on mononuclear leukocytes. Finally, they will determine if expression of these molecules leads to recruitment of mononuclear ..
  31. REGULATION OF BREAST CANCER CELL GROWTH BY ONCOSTATIN M
    Jingwen Liu; Fiscal Year: 2004
    ..differentiative activities of OM on breast cancer cells and its unique source of production by activated mononuclear leukocytes suggest that this cytokine might be involved in a host defense to fight tumor progression in vivo...
  32. NOVEL TRANSFUSION BASED IMMUNE MODULATION
    Edgar G Engleman; Fiscal Year: 2006
    ..of investigators who have developed techniques for isolating, growing and activating rare types of mononuclear leukocytes, and demonstrated their therapeutic effects in model systems...
  33. EXTRAVASCULAR TRAFFICKING OF HIV+ CELLS AND PROGNOSIS
    Holly Birdsall; Fiscal Year: 2003
    ....
  34. Genetics, Endocrinology and PTSD Risk in the Population
    Rachel Yehuda; Fiscal Year: 2006
    ..The development of models of prevention and treatment of PTSD can only occur following an understanding of individual risk factors and their interactions with event exposure. ..
  35. Psychobiology of PTSD: A Decade of Progress
    Rachel Yehuda; Fiscal Year: 2005
    ..We also expect the conference to be attractive to educated lay individuals such as consumers, family members, advocates, and members of the press who are interested in tracking the development of knowledge in this area. ..
  36. Biology of Risk of PTSD in Holocaust Survivor Offspring
    Rachel Yehuda; Fiscal Year: 2006
    ..The subjects will be further subdivided so that there are 40 in each group with and without PTSD; half of each subgroup will have a current depressive disorder. Thus, we will recruit a total of 20 subjects in each of 12 subgroups. ..
  37. Relationship between Bio and Psych Correlates of PTSD
    Rachel Yehuda; Fiscal Year: 2005
    ..abstract_text> ..
  38. Role of Mitochondria in HIV Lipoatrophy
    Grace McComsey; Fiscal Year: 2009
    ..abstract_text> ..
  39. Reconstitution of Protective CMV Immunity and Immune Regulation After HAART
    Adriana Weinberg; Fiscal Year: 2008
    ..These analyses will define the role of TREG in the inadequate CMV immune reconstitution of HAART recipients. [unreadable] [unreadable] [unreadable] [unreadable]..
  40. Metabolic Consequences of Thymidine Sparing Regimens
    Grace A McComsey; Fiscal Year: 2010
    ..at the top of each printed page and each continuation page. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..
  41. HIV-1 Proviral DNA and Monocyte Phenotype in Relation to Neurocognitive Function
    Bruce T Shiramizu; Fiscal Year: 2010
    ..This resource-rich team is well-positioned to conduct this investigation into the pathogenesis of HAD. ..
  42. BIOLOGY OF PEDIATRIC HEPATITIS C INFECTION
    William Borkowsky; Fiscal Year: 2001
    ..The influence of both maternal and child alleles on transmission will be assessed. The correlation of immune responses measured in AIM 3 and the clinical outcome of HCV infection with HLA alleles in the child will be studied. ..
  43. ABI PRISM 7700 SEQUENCE DETECTION SYSTEM
    William Borkowsky; Fiscal Year: 2001
    ..The principal investigator will be responsible for helping design primers and probes for the instrument as well as insure that the assay and those who manage it undergo appropriate quality control procedures. ..
  44. Cellular pharmacology of tenofovir and emtricitabine for HIV prophylaxis
    Peter Anderson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  45. T Cell Immunity & Liver Fibrosis in HCV/HIV Coinfection
    Chihiro Morishima; Fiscal Year: 2003
    ..Finally, we will test the hypotheses that HCV/HIV coinfected subjects treated with HAART will demonstrate improved HCV-specific CD4+ T cell function that is not fibrogenic, and is sequestered to the liver. ..
  46. Activated Immune Parameters Associated with HIV & Aging
    Bruce Shiramizu; Fiscal Year: 2004
    ....
  47. PEDIATRIC AIDS CLINICAL TRIALS UNIT
    William Borkowsky; Fiscal Year: 2002
    ..This effort will also be helped by established links to previously funded community resources, our community advisory board, and by our own local General Clinical Research Center. ..
  48. Pleasant Events and Moods: A Time-Series Analysis
    Brent Mausbach; Fiscal Year: 2006
    ..The remaining participants will receive no feedback. All participants will receive a one-month follow-up to determine if those receiving detailed feedback experience fewer negative emotions and more positive emotions. ..
  49. Genetic-determinants of protease inhibitor pharmacology
    Peter Anderson; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  50. Real-time Minimal Residual Disease assay of Pediatric B-Cell Non-Hodgkin Lymphoma
    Bruce Shiramizu; Fiscal Year: 2007
    ..The assay developed by this exploratory proposal could be incorporated into pediatric B-cell NHL studies to assess MRD on therapy or to assess the efficacy of innovative therapies. [unreadable] [unreadable] [unreadable]..
  51. Uridine Supplementation for HIV lipoatrophy
    Grace McComsey; Fiscal Year: 2007
    ..On-study subjects will continue to be followed per study protocol for a total of 12 months. The last 6 months of the grant will be dedicated to processing of samples and analysis of all data. [unreadable] [unreadable]..
  52. UPLC-MS for pharmacology studies in HIV
    Peter Anderson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  53. TAP-1/E7 INTERACTION: ROLE IN RESPIRATORY PAPILLOMAS
    Andrea Vambutas; Fiscal Year: 2004
    ..To determine whether the interaction between TAP-1 and E7 in naturally occurring infections sufficient to result in a loss of peptide transport and whether this loss correlates with viral protein load and the patients clinical course. ..
  54. PARTIAL OPIOIDS--POTENTIAL TREATMENTS FOR COCAINE ABUSE
    Jean Bidlack; Fiscal Year: 2001
    ..The characterization of novel cyclazocine derivatives will identify potential medications for treating cocaine and heroin abuse and will suggest the basic mechanisms responsible for the drug's action. ..
  55. Determinants of Innate Immunity to Group B Streptococci
    Ofer Levy; Fiscal Year: 2005
    ..abstract_text> ..
  56. Adenosine Shapes Neonatal Toll-like Receptor Function
    Ofer Levy; Fiscal Year: 2010
    ..As a whole, these studies will deepen our understanding of how the unique physiology of the human newborn fundamentally alters innate immunity at birth. ..
  57. PATHOGENESIS OF OROPHARYNGEAL CANDIDIASIS
    Scott Filler; Fiscal Year: 2004
    ..They will determine the contribution of these candidate genes to virulence by constructing of these second generation mutants with oral epithelial cells in vitro and their virulence in the rat model of oropharyngeal infection. ..