blastomeres

Summary

Summary: The undifferentiated cells produced by early cleavages of the fertilized egg (ZYGOTE) inside the intact ZONA PELLUCIDA. Blastomeres are formed during the MORULA and the BLASTULA stages of the preimplantation EMBRYO.

Top Publications

  1. pmc Deterministic and stochastic allele specific gene expression in single mouse blastomeres
    Fuchou Tang
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 6:e21208. 2011
  2. pmc Preclinical validation of a microarray method for full molecular karyotyping of blastomeres in a 24-h protocol
    D S Johnson
    Gene Security Network, Inc, 2686 Middlefield Road, Suite C, Redwood City, CA 94063, USA
    Hum Reprod 25:1066-75. 2010
  3. ncbi Sequential comprehensive chromosome analysis on polar bodies, blastomeres and trophoblast: insights into female meiotic errors and chromosomal segregation in the preimplantation window of embryo development
    Antonio Capalbo
    Centre for Reproductive Medicine, Genera, Clinica Valle Giulia, Via G De Notaris 2b, Rome 00197, Italy
    Hum Reprod 28:509-18. 2013
  4. pmc Developmental bias in cleavage-stage mouse blastomeres
    Inna Tabansky
    The Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA
    Curr Biol 23:21-31. 2013
  5. ncbi Developmental fate and lineage commitment of singled mouse blastomeres
    Chanchao Lorthongpanich
    Mammalian Development Laboratory, Institute of Medical Biology, 8A Biomedical Grove, 06 06 Immunos, Singapore 138648
    Development 139:3722-31. 2012
  6. ncbi Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos
    C E Rocheleau
    Department of Cell Biology, Program in Molecular Medicine, University of Massachusetts Cancer Center, Worcester 01605, USA
    Cell 90:707-16. 1997
  7. pmc Functional interaction between p90Rsk2 and Emi1 contributes to the metaphase arrest of mouse oocytes
    Maria Paola Paronetto
    Department of Public Health and Cell Biology, Section of Anatomy, University of Rome Tor Vergata, Rome, Italy
    EMBO J 23:4649-59. 2004
  8. ncbi The use of morphokinetics as a predictor of embryo implantation
    Marcos Meseguer
    Instituto Valenciano de Infertilidad, Universidad de Valencia, Plaza de la Policia Local, 3, Valencia 46015, Spain
    Hum Reprod 26:2658-71. 2011
  9. pmc SpolvlgA is a DDX3/PL10-related DEAD-box RNA helicase expressed in blastomeres and embryonic cells in planarian embryonic development
    Jordi Solana
    Departament de Genetica, Facultat de Biologia, Universitat de Barcelona, Av Diagonal 645, Edifici annex, Planta 1, 08028 Barcelona, Catalunya, Spain
    Int J Biol Sci 5:64-73. 2009
  10. ncbi Breakage-fusion-bridge cycles leading to inv dup del occur in human cleavage stage embryos
    Thierry Voet
    Center for Human Genetics, KULeuven UZ Gasthuisberg, Herestraat 49, Leuven, Belgium
    Hum Mutat 32:783-93. 2011

Detail Information

Publications259 found, 100 shown here

  1. pmc Deterministic and stochastic allele specific gene expression in single mouse blastomeres
    Fuchou Tang
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 6:e21208. 2011
    ..Here we performed single cell RNA-Seq analysis of single blastomeres of mouse embryos, which revealed significant changes in the transcriptome...
  2. pmc Preclinical validation of a microarray method for full molecular karyotyping of blastomeres in a 24-h protocol
    D S Johnson
    Gene Security Network, Inc, 2686 Middlefield Road, Suite C, Redwood City, CA 94063, USA
    Hum Reprod 25:1066-75. 2010
    ..Preimplantation genetic screening (PGS) has been used in an attempt to determine embryonic aneuploidy. Techniques that use new molecular methods to determine the karyotype of an embryo are expanding the scope of PGS...
  3. ncbi Sequential comprehensive chromosome analysis on polar bodies, blastomeres and trophoblast: insights into female meiotic errors and chromosomal segregation in the preimplantation window of embryo development
    Antonio Capalbo
    Centre for Reproductive Medicine, Genera, Clinica Valle Giulia, Via G De Notaris 2b, Rome 00197, Italy
    Hum Reprod 28:509-18. 2013
    ..optimal stage from oocyte through preimplantation embryo development for biopsy and preimplantation genetic screening (PGS) to detect abnormal chromosome segregation patterns in eggs or embryos from advanced maternal age (AMA) patients?..
  4. pmc Developmental bias in cleavage-stage mouse blastomeres
    Inna Tabansky
    The Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA
    Curr Biol 23:21-31. 2013
    The cleavage-stage mouse embryo is composed of superficially equivalent blastomeres that will generate both the embryonic inner cell mass (ICM) and the supportive trophectoderm (TE)...
  5. ncbi Developmental fate and lineage commitment of singled mouse blastomeres
    Chanchao Lorthongpanich
    Mammalian Development Laboratory, Institute of Medical Biology, 8A Biomedical Grove, 06 06 Immunos, Singapore 138648
    Development 139:3722-31. 2012
    ..Here, we investigate whether cell-cell interaction can influence the fate specification of embryonic blastomeres by sequentially separating the blastomeres in two-cell stage mouse embryos and continuing separation after each ..
  6. ncbi Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos
    C E Rocheleau
    Department of Cell Biology, Program in Molecular Medicine, University of Massachusetts Cancer Center, Worcester 01605, USA
    Cell 90:707-16. 1997
    ..We present evidence that there may be partially redundant inputs into endoderm specification and that a subset of these genes appear also to function in determining cytoskeletal polarity in certain early blastomeres.
  7. pmc Functional interaction between p90Rsk2 and Emi1 contributes to the metaphase arrest of mouse oocytes
    Maria Paola Paronetto
    Department of Public Health and Cell Biology, Section of Anatomy, University of Rome Tor Vergata, Rome, Italy
    EMBO J 23:4649-59. 2004
    ....
  8. ncbi The use of morphokinetics as a predictor of embryo implantation
    Marcos Meseguer
    Instituto Valenciano de Infertilidad, Universidad de Valencia, Plaza de la Policia Local, 3, Valencia 46015, Spain
    Hum Reprod 26:2658-71. 2011
    ..In order to compare a large number of embryos, with minimal variation in culture conditions, we have used an automatic embryo monitoring system...
  9. pmc SpolvlgA is a DDX3/PL10-related DEAD-box RNA helicase expressed in blastomeres and embryonic cells in planarian embryonic development
    Jordi Solana
    Departament de Genetica, Facultat de Biologia, Universitat de Barcelona, Av Diagonal 645, Edifici annex, Planta 1, 08028 Barcelona, Catalunya, Spain
    Int J Biol Sci 5:64-73. 2009
    ..Expression in adult worms is also monitored and is found in the planarian germ line, where it is showed to be expressed in spermatogonia, spermatocytes and differentiating spermatids...
  10. ncbi Breakage-fusion-bridge cycles leading to inv dup del occur in human cleavage stage embryos
    Thierry Voet
    Center for Human Genetics, KULeuven UZ Gasthuisberg, Herestraat 49, Leuven, Belgium
    Hum Mutat 32:783-93. 2011
    ..Based on the copy number changes that were observed in the blastomeres it was hypothesized that chromosome breakages and fusions occur frequently in cleavage stage human embryos and ..
  11. ncbi A quantitative approach to the study of cell shapes and interactions during early chordate embryogenesis
    Olivier Tassy
    IBDM Institut de Biologie du Développement de Marseille, UMR6216, CNRS Université de la Méditerranée, Campus de Luminy, Case 907, 13288 Marseille Cedex 9, France
    Curr Biol 16:345-58. 2006
    ..We applied this approach to early ascidian embryos, chosen because of their simplicity and their phylogenetic proximity to vertebrates...
  12. ncbi Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells
    D N Wells
    AgResearch, Ruakura Research Centre, PB 3123, Hamilton, New Zealand
    Biol Reprod 60:996-1005. 1999
    ..Additionally, some first-generation morulae were recloned by fusing blastomeres to S-phase cytoplasts...
  13. ncbi Furrow-specific endocytosis during cytokinesis of zebrafish blastomeres
    Bo Feng
    Laboratory of Fish Developmental Genetics, Institute of Molecular Agrobiology, National University of Singapore, 1 Research Link, NUS, 117604, Singapore
    Exp Cell Res 279:14-20. 2002
    ..These data suggest that furrow-specific endocytosis is an integral part of cytokinesis...
  14. pmc RNA-Seq analysis to capture the transcriptome landscape of a single cell
    Fuchou Tang
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, UK
    Nat Protoc 5:516-35. 2010
    ..Compared with cDNA microarray techniques, our assay can capture up to 75% more genes expressed in early embryos. This protocol can generate deep-sequencing libraries for 16 single-cell samples within 6 d...
  15. ncbi Stochastic patterning in the mouse pre-implantation embryo
    Jens Erik Dietrich
    Max Planck Institute of Immunobiology, Department of Developmental Biology, Freiburg i Br, Germany
    Development 134:4219-31. 2007
    ..In the first phase, starting after compaction, blastomeres show highly variable Cdx2 and Nanog protein levels...
  16. pmc Dynamic assembly of brambleberry mediates nuclear envelope fusion during early development
    Elliott W Abrams
    Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, 1211 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6058, USA
    Cell 150:521-32. 2012
    To accommodate the large cells following zygote formation, early blastomeres employ modified cell divisions...
  17. ncbi Donor cells at the G1 phase enhance homogeneous gene expression among blastomeres in bovine somatic cell nuclear transfer embryos
    Daisaku Iwamoto
    Department of Genetic Engineering, Kinki University, Kinokawa, Wakayama, Japan
    Cell Reprogram 14:20-8. 2012
    ..Moreover, half of the G1-SCNT embryos exhibited homogeneous luminescence among the blastomeres, whereas more than half of the G0-SCNT embryos exhibited mosaic luminescence...
  18. ncbi Production of loach (Misgurnus anguillicaudatus) germ-line chimera using transplantation of primordial germ cells isolated from cryopreserved blastomeres
    G S Yasui
    Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Sciences, Graduate School of Fisheries Sciences, Hokkaido University, 3 1 1, Hakodate, Hokkaido, 041 8611, Japan
    J Anim Sci 89:2380-8. 2011
    An efficient procedure for the cryopreservation of fish blastomeres followed by restoration through germ-line chimera formation was established...
  19. ncbi (beta)-catenin mediates the specification of endoderm cells in ascidian embryos
    K Imai
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Development 127:3009-20. 2000
    ..and obtained the following results: (1) During cleavages, (beta)-catenin accumulated in the nuclei of vegetal blastomeres, suggesting that it plays a role in the specification of endoderm...
  20. ncbi Origins and rates of aneuploidy in human blastomeres
    Matthew Rabinowitz
    Natera Inc, Gene Security Network, Inc, Redwood City, California, USA
    Fertil Steril 97:395-401. 2012
    ..To characterize chromosomal error types and parental origin of aneuploidy in cleavage-stage embryos using an informatics-based technique that enables the elucidation of aneuploidy-causing mechanisms...
  21. pmc Poor correlations in the levels of pathogenic mitochondrial DNA mutations in polar bodies versus oocytes and blastomeres in humans
    Nadine Gigarel
    Universite Paris Descartes, Faculte de Medecine, Unité INSERM U781, Service de Genetique Medicale, Hôpital Necker Enfants Malades Assistance Publique Hôpitaux de Paris, 149 rue de Sevres, 75743 Paris Cedex 15, France
    Am J Hum Genet 88:494-8. 2011
    ..this point, we compared the mutant levels in 51 first polar bodies (PBs) and their counterpart (oocytes, blastomeres, or whole embryos), at risk of having (1) the "MELAS" m...
  22. ncbi Dynamic microtubules and specification of the zebrafish embryonic axis
    S Jesuthasan
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    Curr Biol 7:31-42. 1997
    ..The early events in development are under the control of maternal genes but are relatively unexplored. Here, the process of axis specification is investigated...
  23. ncbi Single cell CGH analysis reveals a high degree of mosaicism in human embryos from patients with balanced structural chromosome aberrations
    H Malmgren
    Department of Molecular Medicine, Karolinska Institutet, Karolinska Hospital, S 171 76 Stockholm, Sweden
    Mol Hum Reprod 8:502-10. 2002
    We have performed comparative genomic hybridization (CGH) analysis of single blastomeres from human preimplantation embryos of patients undergoing preimplantation genetic diagnosis (PGD) for inherited structural chromosome aberrations ..
  24. ncbi The fate of isolated blastomeres with respect to germ cell formation in the amphipod crustacean Parhyale hawaiensis
    Cassandra G Extavour
    Department of Zoology, Laboratory for Development and Evolution, University of Cambridge, Downing Street, Cambridge, CB2 3EJ Cambridgeshire, United Kingdom
    Dev Biol 277:387-402. 2005
    ..hawaiensis embryogenesis. I determine the capacity of individual blastomeres to form germ cells by isolating blastomeres at early cleavage stages and provide experimental evidence for ..
  25. doi Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos
    Ban Sato
    Department of Developmental Biology, National Research Institute for Child Health and Development, 2 1 0 1 Okura, Setagaya Ku, Tokyo 157 8535, Japan
    Biochem Biophys Res Commun 364:838-43. 2007
    ..that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between ..
  26. ncbi The C. elegans MEX-1 protein is present in germline blastomeres and is a P granule component
    S Guedes
    Department of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Development 124:731-9. 1997
    In the nematode Caenorhabditis elegans, germ cells arise from early embryonic cells called germline blastomeres. Cytoplasmic structures called P granules are present in the fertilized egg and are segregated into each of the germline ..
  27. ncbi mRNA-Seq whole-transcriptome analysis of a single cell
    Fuchou Tang
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, UK
    Nat Methods 6:377-82. 2009
    ..Finally, for Dicer1(-/-) and Ago2(-/-) (Eif2c2(-/-)) oocytes, we found that 1,696 and 1,553 genes, respectively, were abnormally upregulated compared to wild-type controls, with 619 genes in common...
  28. ncbi Differential mitochondrial distribution in human pronuclear embryos leads to disproportionate inheritance between blastomeres: relationship to microtubular organization, ATP content and competence
    J Van Blerkom
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80302 and Colorado Reproductive Endocrinology, Rose Medical Center, Denver, CO 80220, USA
    Hum Reprod 15:2621-33. 2000
    ..oocytes and cleavage stage embryos, followed either by measurements of the net ATP content of individual blastomeres or anti-tubulin immunofluorescence to determine the relationship between mitochondrial distribution and ..
  29. ncbi Chromosome abnormalities identified by comparative genomic hybridization in embryos from women with repeated implantation failure
    L Voullaire
    Murdoch Children s Research Institute, Royal Children s Hospital, Parkville, Victoria 3052, Australia
    Mol Hum Reprod 8:1035-41. 2002
    Using comparative genomic hybridization, we have detected chromosome abnormality in 76/126 (60%) single blastomeres biopsied prior to implantation from embryos from 20 women with repeated implantation failure following IVF...
  30. ncbi Chiral blastomere arrangement dictates zygotic left-right asymmetry pathway in snails
    Reiko Kuroda
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro ku, Tokyo 153 8902, Japan
    Nature 462:790-4. 2009
    ..Similar chiral blastomere configuration mechanisms may also operate upstream of the Nodal pathway in left-right patterning of deuterostomes/vertebrates...
  31. ncbi Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape
    Yoko Kurotaki
    Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Science 316:719-23. 2007
    ..Rather, both the boundary between two-cell blastomeres and the E-Ab axis of the blastocyst align relative to the ellipsoidal shape of the zona pellucida (ZP), an ..
  32. doi Human embryonic stem cell lines generated without embryo destruction
    Young Chung
    Cell Stem Cell 2:113-7. 2008
  33. pmc Functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis
    Amparo Galan
    Valencia Node of The National Stem Cell Bank, Centro de Investigacion Principe Felipe, Valencia, Spain
    PLoS ONE 5:e13615. 2010
    ..Forty-nine blastomeres from 5- to 8-cell human embryos have been investigated following an efficient single-cell cDNA amplification ..
  34. pmc Early, nonciliary role for microtubule proteins in left-right patterning is conserved across kingdoms
    Maria Lobikin
    Department of Biology, Center for Regenerative and Developmental Biology, Tufts University, Medford, MA 02155, USA
    Proc Natl Acad Sci U S A 109:12586-91. 2012
    ..The origin of laterality is cytoplasmic, ancient, and highly conserved across kingdoms, a fundamental feature of the cytoskeleton that underlies chirality in cells and multicellular organisms...
  35. ncbi Sequential signaling crosstalk regulates endomesoderm segregation in sea urchin embryos
    Aditya J Sethi
    National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA
    Science 335:590-3. 2012
    ..Thus, we report that endomesoderm segregation is a progressive process, requiring a succession of regulatory interactions between cWnt and Notch signaling...
  36. ncbi Initial differentiation of blastomeres in 4-cell human embryos and its significance for early embryogenesis and implantation
    Robert G Edwards
    Reproductive BioMedicine Online, Duck End Farm, Dry Drayton, Cambridge CB3 8DB, UK
    Reprod Biomed Online 11:206-18. 2005
    ..Knowledge of the properties of individual blastomeres should help in improving assisted human reproduction, performing preimplantation genetic diagnosis, and perhaps ..
  37. pmc Normal bias in the direction of fetal rotation depends on blastomere composition during early cleavage in the mouse
    Richard L Gardner
    Department of Biology, University of York, York, United Kingdom
    PLoS ONE 5:e9610. 2010
    ..Reconstructing cleavage stages with correspondingly positioned blastomeres from appropriate numbers of conceptuses with similar division planes provides a way of testing whether they ..
  38. ncbi Does the S phase have an impact on the accuracy of comparative genomic hybridization profiles in single fibroblasts and human blastomeres?
    Laia Ramos
    Unitat de Biologia Cel lular i Genètica Mèdica Facultat de Medicina, Departament de Biologia Cel lular, Fisiologia i Immunologia, Universitat Autonoma de Barcelona, Bellaterra, Spain
    Fertil Steril 101:488-95. 2014
    ..To investigate if there is an association between single-cell replicative stage and the segmental chromosome imbalances detected by comparative genomic hybridization (CGH)...
  39. ncbi Identical triplets and twins developed from isolated blastomeres of 8- and 16-cell mouse embryos supported with tetraploid blastomeres
    Andrzej K Tarkowski
    Department of Embryology, Institute of Zoology, Faculty of Biology, Warsaw University, Warsaw, Poland
    Int J Dev Biol 49:825-32. 2005
    We studied the developmental potential of single blastomeres from early cleavage mouse embryos...
  40. ncbi Predictability of dorso-ventral asymmetry in the cleavage stage zebrafish embryo: an analysis using lithium sensitivity as a dorso-ventral marker
    P Aanstad
    Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, UK
    Mech Dev 88:33-41. 1999
    ..Consequently, the dorso-ventral axis corresponds to the long axis of the embryo. Because the effect of lithium treatment is short-lived, the dorso-ventral axis must be specified in zebrafish already at the 32 cell stage...
  41. ncbi Production of chimeric embryos by aggregation of bovine egfp eight-cell stage blastomeres with two-cell fused and asynchronic embryos
    M I Hiriart
    Laboratorio de Biotecnología Animal, University of Buenos Aires, Buenos Aires, Argentina
    Theriogenology 80:357-64. 2013
    ..This technique will have great application for multiplication of embryos of high genetic value or transgenic embryos and also with the generation of truly bovine embryonic stem cells and induced pluripotent stem cells...
  42. pmc Reprogramming after chromosome transfer into mouse blastomeres
    Dieter Egli
    Stowers Medical Institute, Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02140, USA
    Curr Biol 19:1403-9. 2009
    ..Here, we used chromosome transplantation techniques to investigate whether the blastomeres of two-cell-stage mouse embryos can reprogram more differentiated cells...
  43. ncbi Binucleated and micronucleated blastomeres in embryos derived from human assisted reproduction cycles
    James Meriano
    LifeQuest Centre for Reproductive Medicine, 655 Bay St Suite 1800 18th Floor, Toronto, Ontario, M5G 2K4, Canada
    Reprod Biomed Online 9:511-20. 2004
    ..common multinucleation phenotypes observed in the authors' clinic, binucleated (BN) and micronucleated (MN) blastomeres, and included all intracytoplasmic sperm injection (ICSI) patients <40 years of age with at least one ..
  44. ncbi Spatial arrangement of individual 4-cell stage blastomeres and the order in which they are generated correlate with blastocyst pattern in the mouse embryo
    Karolina Piotrowska-Nitsche
    Wellcome Trust Cancer Research UK, Gurdon Institute, Tennis Court Road, Cambridge CB2 1QR, UK
    Mech Dev 122:487-500. 2005
    In the unperturbed development of the mouse embryo one of the 2-cell blastomeres tends to contribute its progeny predominantly to the embryonic and the other to the abembryonic part of the blastocyst...
  45. ncbi Xenopus p21-activated kinase 5 regulates blastomeres' adhesive properties during convergent extension movements
    Sandrine Faure
    Centre de Recherches en Biochimie Macromoléculaire, FRE 2593 CNRS, 34293 Montpellier, France
    Dev Biol 277:472-92. 2005
    ..Taken together, our results suggest that X-PAK5 regulates convergent extension movements in vivo by modulating the calcium-mediated cell-cell adhesion...
  46. ncbi Sex determination of bovine embryo blastomeres by fluorogenic probes
    J Virta
    Animal Production Research, Animal Breeding, MTT Agrifood Research, Jokioinen, Finland
    Theriogenology 57:2229-36. 2002
    ..preamplification, polymerase chain reaction (PCR) method for the determination of sex in bovine embryo blastomeres. For this aim, ZFX/ZFY sequences were preamplified simultaneously with other genomic regions...
  47. ncbi Removal of vegetal yolk causes dorsal deficencies and impairs dorsal-inducing ability of the yolk cell in zebrafish
    T Mizuno
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya, 464 8602, Japan
    Mech Dev 81:51-63. 1999
    ..the yolk cell obtained from the vegetal-removed embryos lost the ability to induce goosecoid in normal blastomeres while the normal yolk cell frequently did so in normal and vegetal-removed embryos...
  48. pmc Reprogramming of early embryonic blastomeres into endodermal progenitors by a Caenorhabditis elegans GATA factor
    J Zhu
    Department of Molecular, Cellular, and Developmental Biology and Neuroscience Research Institute, University of California, Santa Barbara, California 93106 USA
    Genes Dev 12:3809-14. 1998
    ..This suggests that a primary function of these maternal factors is to regulate zygotic end-1 expression, which is then sufficient to initiate the entire program for endoderm development...
  49. ncbi Tight junctions in early amphibian development: detection of junctional cingulin from the 2-cell stage and its localization at the boundary of distinct membrane domains in dividing blastomeres in low calcium
    P Cardellini
    Dipartimento di Biologia, Universita di Padova, Italy
    Dev Dyn 207:104-13. 1996
    ..In embryos incubated in low calcium medium for 30 min, newly divided blastomeres failed to completely adhere to each other...
  50. pmc Mouse cloning and somatic cell reprogramming using electrofused blastomeres
    Amjad Riaz
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 1st Beichen West Road, Beijing 100101, China
    Cell Res 21:770-8. 2011
    ..embryos, arrested in mitosis, can support developmental reprogramming of nuclei from donor cells ranging from blastomeres to somatic cells...
  51. ncbi Transcriptome fingerprint of bovine 2-cell stage blastomeres is directly correlated with the individual developmental competence of the corresponding sister blastomere
    Eva Held
    Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany
    Biol Reprod 87:154. 2012
    ..This study sought to find a direct correlation between transcriptome fingerprint of blastomeres of bovine 2-cell stage embryos with developmental competence of the corresponding sister blastomeres...
  52. ncbi Contribution of early cells to the fate map of the zebrafish gastrula
    K A Helde
    Department of Human Genetics, Eccles Institute of Human Genetics, University of Utah, Salt Lake City 84112
    Science 265:517-20. 1994
    ..because of variability in both the position of the future dorsoventral axis with respect to the early cleavage blastomeres and the scattering of daughter cells as the gastrula is formed...
  53. ncbi Conversion of zebrafish blastomeres to an endodermal fate by TGF-beta-related signaling
    N Peyrieras
    INSERM U368, Ecole Normale Superieure, Paris, France
    Curr Biol 8:783-6. 1998
    ..Activation of TARAM-A was found to drive blastomeres towards an endodermal fate...
  54. ncbi Internalization of cellular fragments in a human embryo: time-lapse recordings
    T Hardarson
    Department of Obstetrics and Gynaecology, Goteborg University, SU Sahlgrenska, 413 45 Gothenburg, Sweden
    Reprod Biomed Online 5:36-8. 2002
    ..The fragment, created during the first mitotic cleavage was reabsorbed into one of the mother blastomeres in less than 5 min...
  55. ncbi Laser zona drilling does not induce hsp70i transcription in blastomeres of eight-cell mouse embryos
    Cristina Hartshorn
    Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Fertil Steril 84:1547-50. 2005
    ..with a 1,480-nm laser induces heat shock in eight-cell embryos, we measured hsp70i RNA levels in sets of single blastomeres isolated after laser treatment of mouse embryos that had or had not been heated at 43 degrees C...
  56. ncbi Differential expression of Axin1, Cdc25c and Cdkn2d mRNA in 2-cell stage mouse blastomeres
    Jian hong Sun
    Institute of Biological Engineering, College of Veterinary Medicine, Northwest A and F University, Yangling, Shaanxi Province 712100, P R China
    Zygote 20:305-10. 2012
    There is increasing evidence to show that 2-cell stage mouse blastomeres have differing developmental properties...
  57. ncbi Cryopreservation of chum salmon blastomeres by the straw method
    Satoshi Kusuda
    Hokkaido Fish Hatchery, Kitakashiwagi, Eniwa, Hokkaido 061 1433, Japan
    Cryobiology 45:60-7. 2002
    ..genetic resources of chum salmon, Oncorhynchus keta, optimum conditions for cryopreservation of isolated blastomeres were investigated...
  58. ncbi Embryo quality and impact of specific embryo characteristics on ongoing implantation in unselected embryos derived from modified natural cycle in vitro fertilization
    Marie José Pelinck
    Department of Obstetrics and Gynaecology, University Medical Center Groningen, Groningen, The Netherlands
    Fertil Steril 94:527-34. 2010
    ..To study the implantation potential of unselected embryos derived from modified natural cycle IVF according to their morphological characteristics...
  59. ncbi Delay of polarization event increases the number of Cdx2-positive blastomeres in mouse embryo
    Ilona Kondratiuk
    Department of Embryology, Institute of Zoology, University of Warsaw, Miecznikowa 1, 02 096 Warsaw, Poland
    Dev Biol 368:54-62. 2012
    ..Such embryos developed from the blastomeres that were isolated from 8-cell embryos either before or after the compaction, i.e...
  60. ncbi Effect of fragment removal on blastocyst formation and quality of human embryos
    Poopak Eftekhari-Yazdi
    Department of Anatomy, School of Medical Science, Tarbiat Modarres University, PO Box 14115 111, Tehran, Iran
    Reprod Biomed Online 13:823-32. 2006
    ..end of day 6 of culture, in the experimental group especially in grade IV blastocyst rate, size and number of blastomeres in each blastocyst were all improved compared with those of the control group (42.3 versus 20.0%; 19,205...
  61. pmc Transcript profiling of individual twin blastomeres derived by splitting two-cell stage murine embryos
    R Michael Roberts
    Division of Animal Sciences, Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA
    Biol Reprod 84:487-94. 2011
    ..Here we examined whether twin blastomeres from 2-cell stage mouse embryos differ in mRNA content...
  62. pmc Transcriptome asymmetry within mouse zygotes but not between early embryonic sister blastomeres
    Matthew D VerMilyea
    Laboratory of Mammalian Molecular Embryology, RIKEN Center for Developmental Biology, Kobe, Japan
    EMBO J 30:1841-51. 2011
    ..Accordingly, there is transcriptome asymmetry within mouse oocytes, but not between the sister blastomeres of early embryos...
  63. pmc Genome-wide microarray evidence that 8-cell human blastomeres over-express cell cycle drivers and under-express checkpoints
    Ann A Kiessling
    Bedford Stem Cell Research Foundation, 206 Elm St, Suite 106, Somerville, MA 02144, USA
    J Assist Reprod Genet 27:265-76. 2010
    ..To understand cell cycle controls in the 8-Cell human blastomere...
  64. ncbi Towards defining parameters for a successful single embryo transfer in frozen cycles
    R Tang
    Monash IVF, Clayton, Victoria, Australia
    Hum Reprod 21:1179-83. 2006
    ..In this study, we investigated the effect of blastomere lysis and cleavage in singleton frozen embryo transfer (sFET) cycles. Outcomes were compared with the transfer of two embryos in frozen transfer cycles (dFET)...
  65. ncbi Use of picosecond infrared laser for micromanipulation of early mammalian embryos
    Artashes V Karmenyan
    Institute of Biophotonics, National Yang Ming University, Taipei, Taiwan
    Mol Reprod Dev 76:975-83. 2009
    ..for the inactivation (functional enucleation) of oocytes and two-cell mouse embryos and also for the fusion of blastomeres of two-cell mouse embryos...
  66. ncbi Improvement of the developmental ability of nuclear transfer embryos by using blastomeres from in vitro fertilized embryos selected according to the early developmental stage and cell division status as donor cells in cattle
    Yuji Goto
    National Institute of Livestock and Grassland Science, Nagano, Japan
    J Reprod Dev 57:249-55. 2011
    This study was conducted to improve the developmental ability of nuclear transfer (NT) embryos by using blastomeres from in vitro fertilized (IVF) embryos with high quality as donor cells...
  67. ncbi The neglected morula/compact stage embryo transfer
    Jun Tao
    Arizona Institute of Reproductive Medicine, Phoenix, Arizona, USA
    Hum Reprod 17:1513-8. 2002
    ..This retrospective study analysed the outcomes of 339 embryo transfers on either day 3 (n = 97) or day 4 (n = 242), and proposed a grading system for morula/compact embryos...
  68. ncbi Cryopreservation of zebrafish (Danio rerio) blastomeres by controlled slow cooling
    C Lin
    LIRANS Institute of Research in the Applied Natural Sciences, University of Bedfordshire, Bedfordshire, UK
    Cryo Letters 30:132-41. 2009
    ..This study sets out to determine an optimum cryopreservation protocol for blastomeres isolated from 50% epiboly zebrafish embryos. Freezing was performed in 0.25 ml straws in a programmable freezer...
  69. ncbi Maternal transcripts of mitotic checkpoint gene, Xbub3, are accumulated in the animal blastomeres of Xenopus early embryo
    T Goto
    Developmental Biology, Faculty of Science, Kwansei Gakuin University, Nishinomiya, Japan
    DNA Cell Biol 18:227-34. 1999
    ..We compared the quantity of mRNAs among four blastomeres of Xenopus 8-cell-stage embryos by the differential display method...
  70. ncbi National collection of embryo morphology data into Society for Assisted Reproductive Technology Clinic Outcomes Reporting System: associations among day 3 cell number, fragmentation and blastomere asymmetry, and live birth rate
    Catherine Racowsky
    Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Fertil Steril 95:1985-9. 2011
    ..To evaluate the validity of collecting day 3 embryo morphology variables into the Society for Assisted Reproductive Technology Clinic Outcomes Reporting System (SART CORS)...
  71. pmc The competence of Xenopus blastomeres to produce neural and retinal progeny is repressed by two endo-mesoderm promoting pathways
    Bo Yan
    Department of Anatomy and Cell Biology, The George Washington University Medical Center, 2300 Eye Street, N W, Washington, DC 20037, USA
    Dev Biol 305:103-19. 2007
    Only a subset of cleavage stage blastomeres in the Xenopus embryo is competent to contribute cells to the retina; ventral vegetal blastomeres do not form retina even when provided with neuralizing factors or transplanted to the most ..
  72. ncbi Number of blastomeres and distribution of microvilli in cloned mouse embryos during compaction
    Chao Bo Li
    Department of Histology and Embryology, Harbin Medical University, Harbin, China
    Zygote 19:271-6. 2011
    ..by somatic cell nuclear transfer (SCNT) in aspects different from previous studies, not only the number of blastomeres of cloned embryos during the initiation of compaction, but also the distribution of microvilli in cloned, ..
  73. pmc Spatial alignment of the mouse blastocyst axis across the first cleavage plane is caused by mechanical constraint rather than developmental bias among blastomeres
    Vernadeth B Alarcon
    Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, John A Burns School of Medicine at Kakaako, University of Hawaii, Honolulu, Hawaii 96813, USA
    Mol Reprod Dev 75:1143-53. 2008
    ..it is also possible that such alignment is not due to any developmental disparity between the two-cell stage blastomeres, but rather is caused by an extrinsic mechanical constraint that is conferred by an irregular shape of the zona ..
  74. ncbi Rhesus monkey embryos produced by nuclear transfer from embryonic blastomeres or somatic cells
    Shoukhrat M Mitalipov
    Oregon Regional Primate Research Center, Beaverton, OR 97006, USA
    Biol Reprod 66:1367-73. 2002
    ..study was to determine the developmental competence of nuclear transfer (NT) embryos derived from embryonic blastomeres (embryonic cell NT) or fetal fibroblasts (somatic cell NT) as a first step in the production of rhesus monkeys ..
  75. ncbi Midblastula transition (MBT) of the cell cycles in the yolk and pigment granule-free translucent blastomeres obtained from centrifuged Xenopus embryos
    Yasuhiro Iwao
    Department of Biological Science, Faculty of Science, Yamaguchi University, 753 8512 Yamaguchi, Japan
    Dev Growth Differ 47:283-94. 2005
    We obtained translucent blastomeres free of yolk and pigment granules from Xenopus embryos which had been centrifuged at the beginning of the 8-cell stage with cellular integrity...
  76. ncbi Binary specification of the embryonic lineage in Caenorhabditis elegans
    T Kaletta
    Max Planck Institut fur Biochemie, Martinsried, Germany
    Nature 390:294-8. 1997
    ..A central problem in embryonic development is to understand how the developmental potential of blastomeres is restricted to permit the terminal expression of such complex differentiation patterns...
  77. pmc A targeted mutation in the mouse E-cadherin gene results in defective preimplantation development
    D Riethmacher
    Max Delbrueck Laboratorium, Max Planck Gesellschaft, Koeln, Germany
    Proc Natl Acad Sci U S A 92:855-9. 1995
    ..Thus, maternal E-cadherin suffices for initial compaction of the morula but not for further preimplantation development to occur...
  78. ncbi Ultrastructural morphometry of bovine blastocysts produced in vivo or in vitro
    A E Crosier
    Departments of Animal Science, North Carolina State University, Raleigh 27695-7621, USA
    Biol Reprod 64:1375-85. 2001
    ..However, the specific nature of these deviations varied with the type of culture conditions used for in vitro embryo production...
  79. pmc Histone arginine methylation regulates pluripotency in the early mouse embryo
    Maria Elena Torres-Padilla
    The Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 445:214-8. 2007
    ..Levels of H3 methylation at specific arginine residues are maximal in four-cell blastomeres that will contribute to the inner cell mass (ICM) and polar trophectoderm and undertake full development when ..
  80. ncbi Wnt signaling polarizes an early C. elegans blastomere to distinguish endoderm from mesoderm
    C J Thorpe
    Institute of Molecular Biology, University of Oregon, Eugene 97403, USA
    Cell 90:695-705. 1997
    ..Defective mitotic spindle orientations in mom mutant embryos indicate that Wnt signaling influences cytoskeletal polarity in blastomeres throughout the early embryo.
  81. pmc Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell mass in the mouse embryo
    Agnieszka Jedrusik
    Wellcome Trust Cancer Research UK Gurdon Institute, Cambridge CB2 1QN, United Kingdom
    Genes Dev 22:2692-706. 2008
    ..It is initiated via asymmetric divisions of eight- and 16-cell blastomeres that allocate cells to inner and outer positions, each with different developmental fates...
  82. ncbi Markers that define stemness in ESC are unable to identify the totipotent cells in human preimplantation embryos
    G Cauffman
    Department of Embryology and Genetics, UZ Brussel and the Vrije Universiteit Brussel, Laarbeeklaan 101, Brussels 1090, Belgium
    Hum Reprod 24:63-70. 2009
    During human preimplantation development, early blastomeres are believed to be totipotent. It is likely, however, that blastomeres are allocated to a specific lineage prior to any morphological differentiation...
  83. pmc Human embryonic stem cell lines derived from single blastomeres of two 4-cell stage embryos
    Mieke Geens
    Department of Embryology and Genetics, Vrije Universiteit Brussel, 1090 Brussels, Belgium
    Hum Reprod 24:2709-17. 2009
    Recently, we demonstrated that single blastomeres of a 4-cell stage human embryo are able to develop into blastocysts with inner cell mass and trophectoderm...
  84. ncbi skn-1, a maternally expressed gene required to specify the fate of ventral blastomeres in the early C. elegans embryo
    B Bowerman
    Department of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    Cell 68:1061-75. 1992
    ..We propose that the maternally expressed skn-1 gene product acts to specify the fate of the EMS blastomere...
  85. pmc The development of novel quantification assay for mitochondrial DNA heteroplasmy aimed at preimplantation genetic diagnosis of Leigh encephalopathy
    Hiroto Tajima
    Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan
    J Assist Reprod Genet 24:227-32. 2007
    ..To perform preimplantation genetic diagnosis (PGD) of Leigh encephalopathy, we developed a rapid and reliable quantification assay for the percentage of T8993G mtDNA mutation and analyzed various specimens...
  86. ncbi Milder ovarian stimulation for in-vitro fertilization reduces aneuploidy in the human preimplantation embryo: a randomized controlled trial
    Esther B Baart
    Department of Reproductive Medicine and Gynaecology, University Medical Center, Utrecht, The Netherlands
    Hum Reprod 22:980-8. 2007
    ....
  87. ncbi Stem cells and regeneration in planarians
    Mette Handberg-Thorsager
    Genetics Department, University of Barcelona, Av Diagonal 645, Barcelona, Spain
    Front Biosci 13:6374-94. 2008
    ..In the last ten years, molecular, cellular, and bioinformatics tools have been established for use in this model, making it ideally placed for in vivo analysis of stem cells in their natural environment without ethical complications...
  88. ncbi Reliability of short comparative genomic hybridization in fibroblasts and blastomeres for a comprehensive aneuploidy screening: first clinical application
    M Rius
    Unitat de Biologia Cellular i Genetica Medica, Facultat de Medicina, Departament de Biologia Cellular, Fisiologia i Immunologia, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain
    Hum Reprod 25:1824-35. 2010
    ..The aim of this study is to reduce the hybridization period of CGH, in order to make this short-CGH technique suitable for PGS of Day-3 embryos, avoiding the cryopreservation step...
  89. ncbi Parameters guiding selection of best embryos for transfer after cryopreservation: a reappraisal
    Fabrice Guerif
    Biologie de la Reproduction, département de gynécologie obstétrique et reproduction humaine, CHU Bretonneau, 37044 Tours, France
    Hum Reprod 17:1321-6. 2002
    ..The purpose of this study was to evaluate the respective influences of blastomere survival and resumption of mitosis on the outcome of frozen-thawed embryos...
  90. ncbi Preimplantation genetic diagnosis and chromosome analysis of blastomeres using comparative genomic hybridization
    Leeanda Wilton
    Genetic and Molecular Research, Melbourne IVF, 320 Victoria Parade, East Melbourne 3002, Victoria, Australia
    Hum Reprod Update 11:33-41. 2005
    ..CGH has been applied to clinical PGD and has resulted in the birth of healthy babies from embryos whose full karyotype was determined in the preimplantation phase...
  91. ncbi Assessment of beta-HCG, beta-LH mRNA and ploidy in individual human blastomeres
    Christoph Hansis
    Program for In Vitro Fertilization, Reproductive Surgery and Infertility, New York University School of Medicine, 660 First Avenue, 5th Floor, New York, NY 10016, USA
    Reprod Biomed Online 5:156-61. 2002
    In human embryos, blastomeres differentiate into trophectoderm (TE) cells and inner cell mass (ICM) cells of blastocysts...
  92. ncbi A role for Rho-like GTPases in the polarisation of mouse eight-cell blastomeres
    L Clayton
    Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3DY, United Kingdom
    Dev Biol 205:322-31. 1999
    Polarisation of cells during mouse preimplantation development first occurs within blastomeres at the eight-cell stage, as part of a process called compaction...
  93. ncbi Derivation of the first Swiss human embryonic stem cell line from a single blastomere of an arrested four-cell stage embryo
    Anis Feki
    Laboratory of Stem Cell Research, Department of Obstetrics and Gynaecology, Geneva University Hospitals, Geneva, Switzerland
    Swiss Med Wkly 138:540-50. 2008
    ..Moreover, most of the lines have been obtained in media containing animal components such as foetal bovine serum. We aimed to derive hESC lines in xeno-free conditions using spare embryos frozen in Switzerland before 2001...
  94. ncbi Derivation of human embryonic stem cell lines from biopsied blastomeres on human feeders with minimal exposure to xenomaterials
    Dusko Ilic
    SLLSciences StemLifeLine, Inc, San Carlos, California 94070, USA
    Stem Cells Dev 18:1343-50. 2009
    ..In this study, we describe an improved method for efficient derivation of hESC lines from blastomeres of biopsied embryos...
  95. ncbi Individual blastomeres of 16- and 32-cell mouse embryos are able to develop into foetuses and mice
    Andrzej K Tarkowski
    Department of Embryology, University of Warsaw, Miecznikowa 1, 02 096 Warsaw, Poland
    Dev Biol 348:190-8. 2010
    ..To address this question, we studied the developmental potential of single blastomeres derived from 16- and 32-cell stage embryos and supported by carrier, tetraploid blastomeres...
  96. ncbi Effect of blastomere loss on the outcome of frozen embryo replacement cycles
    Tarek El-Toukhy
    Assisted Conception Unit, Guy s and St Thomas Hospital NHS Trust, London, United Kingdom
    Fertil Steril 79:1106-11. 2003
    To assess the impact of survival of cryopreservation and thawing with all blastomeres intact on the outcome of frozen embryo replacement (FER) cycles.
  97. ncbi Potential use of repeated fluorescence in situ hybridization in the same human blastomeres for preimplantation genetic diagnosis
    J Liu
    The Greater Baltimore Medical Center Fertility Center, Maryland 21204, USA
    Fertil Steril 70:729-33. 1998
    ..To assess the feasibility of repeated fluorescence in situ hybridization (FISH) procedures in the same nucleus of a human blastomere...
  98. ncbi Comparison of mitochondrial DNA contents in human embryos with good or poor morphology at the 8-cell stage
    David Pei Cheng Lin
    Institute of Biochemistry, Chung Shan Medical University, ROC, Taichung, Taiwan
    Fertil Steril 81:73-9. 2004
    ..To quantify the mitochondrial DNA contents in human embryos with good or poor quality at the 8-cell stage...
  99. ncbi A default mechanism of spindle orientation based on cell shape is sufficient to generate cell fate diversity in polarised Xenopus blastomeres
    Bernhard Strauss
    The Wellcome Trust Cancer Research UK Gurdon Institute, Cambridge, UK
    Development 133:3883-93. 2006
    ..also take place in the frog blastula, where orientation of the spindle into the apicobasal axis of polarised blastomeres generates inner and outer cells with different fates...
  100. ncbi Differential pattern of Xist RNA accumulation in single blastomeres isolated from 8-cell stage mouse embryos following laser zona drilling
    Cristina Hartshorn
    Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Mol Reprod Dev 64:41-51. 2003
    ..that Xist transcripts are localized on the silenced X chromosome, forming aggregates of variable dimensions in blastomeres of 8-cell embryos...
  101. ncbi Embryonic stem cells from morula
    Nick Strelchenko
    Reproductive Genetics Institute, Chicago, Illinois, USA
    Methods Enzymol 418:93-108. 2006
    ..Technical approaches of freezing morula-derived hESC are discussed...

Research Grants62

  1. Bioelectrical Controls of Morphogenesis
    Michael Levin; Fiscal Year: 2010
    ..unique opportunity to resolve the major puzzle of how early, cytoplasmic events that set up asymmetry in large blastomeres aligned with the embryo's midline may function in other animals (such as mammals) where many small cells ..
  2. CCCH zinc-finger proteins and transcriptional repression in germline blastomeres
    Rueyling Lin; Fiscal Year: 2012
    ..Although PIE-1 protein is in all germline blastomeres, it does not account for repression in P0 or P1...
  3. Novel Roles of Pluripotency Regulators in the Early Mouse Embryo
    Wing H Wong; Fiscal Year: 2012
    ..pluripotency and the ability to self-renew in order to give rise to cell lineages that form the fetus, the blastomeres of the early embryo must establish totipotency and self-renewal abilities to form the blastocyst...
  4. Non-invasive Aneuploidy Screening of Circulating Fetal Cells for Prenatal Diagnos
    Matthew Rabinowitz; Fiscal Year: 2012
    ..commercialized an innovative single cell molecular karyotyping protocol to enable genetic analysis of single blastomeres within 24 hours...
  5. Gene Expression in the Preimplantation Mouse Embryo
    Richard M Schultz; Fiscal Year: 2013
    ..by applicant): The egg-to-embryo transition entails transforming a highly differentiated oocyte into totipotent blastomeres, and represents one of the earliest obstacles that must be successfully hurdled for continued development...
  6. Mechanisms of Contact-Mediated Cell Polarization in the C. elegans Embryo.
    JEREMY FRANKLIN NANCE; Fiscal Year: 2013
    ..These include the polarization of embryonic blastomeres that occurs during compaction and is required for specification of the inner cell mass (which gives rise to the ..
  7. Bioelectrical controls of left-right asymmetry
    Laura N Vandenberg; Fiscal Year: 2010
    ..However, this mechanism relies on the fact that in Xenopus, the cleavage plane of the first large blastomeres normally coincides with the prospective midline of the whole embryo...
  8. Regulators of Dishevelled Function in the Wnt Signaling Pathway
    Athula H Wikramanayake; Fiscal Year: 2013
    ..pole, and these structures correlate with highly restricted activation of Wnt/??-catenin signaling in vegetal blastomeres. In addition, functional studies have shown that Dvl is only able to activate Wnt/??-catenin signaling in ..
  9. Investigating FGF/sFRP interactions during forebrain evolution and development
    ARIEL MATTHEW PANI; Fiscal Year: 2012
    ..Specific Aims I and II will utilize gene knockdowns, mRNA injections into blastomeres, and embryological manipulations of hemichordate embryos to further investigate the evolutionary origins of the ..
  10. Mechanisms of Intracellular Scaling
    Rebecca W Heald; Fiscal Year: 2013
    ..stages of Xenopus laevis embryogenesis, as the ~1 millimeter diameter egg rapidly cleaves to form smaller blastomeres, which by the 15th division are reduced to 40 microns across...
  11. NIH Director's Pioneer Award
    Rebecca W Heald; Fiscal Year: 2010
    ..and other compartments in Xenopus laevis, as the ~1 millimeter diameter egg rapidly cleaves to form smaller blastomeres, which by the 15th division are reduced to 40 microns across...
  12. Novel Informatics for Highly Reliable Multi-Locus Allele Calling for Embryo Scree
    Matthew Rabinowitz; Fiscal Year: 2009
    ..IVF Center, Boston IVF and Huntington Reproductive Center that applies the techniques from aims 1 and 2 to real blastomeres in the IVF context and compare our predictions with truth measured on the child when born...
  13. MOLECULAR ANALYSIS OF CONGENITAL ANOPHTHALMIA
    Thomas Glaser; Fiscal Year: 2004
    ..has the potential to promote neural and pigmented retina formation when ectopically expressed in frog anterior blastomeres (Mathers et al. 1997, Furukawa et al. 1997)...
  14. Mechanisms of Asymmetric RNA segregation in C. elegans Development
    Jason D Lieb; Fiscal Year: 2013
    ..elegans embryo, to characterize the mechanisms of asymmetric distribution of cellular contents in specific blastomeres with modern genomic tools and analysis, followed by tests of function...
  15. ANALYSIS OF CELL LINEAGE IN EMBRYOS
    Roger Pedersen; Fiscal Year: 1992
    ..be performed using microinjected tracers at cleavage and gastrula stages to determine the fate of individual blastomeres in forming the unilaminar marsupial blastocyst, and of individual epiblast cells in forming the primary germ ..
  16. PAF AND PRE-IMPLANTATION/IMPLANTATION PHENOMENA
    Michael Harper; Fiscal Year: 1991
    ..and capacities in relation to embryonic growth and development, and determine their localization on the blastomeres in early stage embryos, and on the cells of the trophoblast and the inner cell mass at the blastocyst stage, 4) ..
  17. RAPID PRENATAL SEXING OF CLEAVING MOUSE EMBRYOS
    JON GORDON; Fiscal Year: 1992
    ..The technique for mouse embryo sexing will be based upon the polymerase chain reaction (PCR). Single blastomeres will be removed from embryos, boiled, and annealed with synthetic oligonucleotides corresponding to a Y-..
  18. The Role of Ductin in Embryonic Morphogenesis
    Michael Levin; Fiscal Year: 2007
    ..data showing that ductin is present in the cell membrane (as well as the cytoplasm) of early embryonic blastomeres consistent with a gap junctional role for ductin...
  19. METABOLIC TESTS OF VIABILITY IN PREIMPLANTATION EMBRYOS
    James Butler; Fiscal Year: 1992
    ..Experiments will be carried out to examine the variation in staining between embryos and between blastomeres of individual embryos...
  20. FUNCTION OF ELECTRICAL COUPLING IN EMBRYONIC TISSUE
    Michael Bennett; Fiscal Year: 1991
    ..Gap junctions in the ascidians show voltage dependence and the blastomeres exhibit two stable resting potentials sufficiently far apart to shut down junctional conductance and uncouple ..
  21. CELL SURFACE ARCHITECTURE OF EMBRYOS AND SPERM
    MARTHA SPIEGELMAN; Fiscal Year: 1980
    ..transmission EM views show that the cell surface is abnormal in tw32/tw32 morulae; microvilli of neighboring blastomeres do not become closely intertwined as in normal morulae that are undergoing compaction...
  22. BLASTULA ADHESION COMPLEXES
    MERRIL HILLE; Fiscal Year: 1990
    ..adhesion complex during development, and the relationship between these changes and the adhesion of blastomeres. (3) To determine the functional domains of this adhesion complex, and the structural requirements for adhesion ..
  23. INTERACTIONS BETWEEN F-ACTIN, MYOSIN II AND MICROTUBULES
    Garrett Odell; Fiscal Year: 2001
    ..will be extended to make a similar description of wildtype F-actin/myosin II/microtubule dynamics in early blastomeres of C...
  24. Mitochondria distribution in cloned pig embryos
    Heide Schatten; Fiscal Year: 2005
    ..Our specific aims are to analyze: 1a) whether differences in mitochondrial distribution occur among individual blastomeres in cohorts of morphologically normal cleavage stage embryos; 1b) whether changes in intracellular pH are ..
  25. MULTIPHOTON LASER SCANNING MICROSCOPE
    JAMES TRUMAN; Fiscal Year: 2000
    ..elegans blastomeres; the dynamics of patterning gene during regeneration in Drosophila imaginal discs; the analysis of steroid ..
  26. ANIMAL MODELS BASED ON NUCLEAR TRANSFER IN THE RAT
    Philip Iannaccone; Fiscal Year: 2000
    ..Recently, nuclear transfer has been shown to produce viable offspring from isolated blastomeres of several mammalian species...
  27. CORTICAL ORGANIZATION IN PREIMPLANTATION MOUSE EMBRYOS
    JAEL SOBEL; Fiscal Year: 1990
    ..of actin filaments, promote microtubule growth, and function in the formation of the polar body; 2) Compacting blastomeres to determine the role of the filaments in surface polarization and blastomere spreading; 3) Blastocysts to ..
  28. EGG CYTOPLASMIC INFORMATION IN CELL DIFFERENTIATION
    J Whittaker; Fiscal Year: 1992
    ..by their differential segregation during early cell divisions into restricted cell lineages: cleavage stage blastomeres in isolation develop and self-differentiate only according to already fixed fates...
  29. NEURONAL LINEAGE DETERMINANTS IN EMBRYOS
    SALLY MOODY; Fiscal Year: 1993
    ..identify and clone cytoplasmic mRNAs or proteins that are involved in neuronal lineage determination of certain blastomeres. The RCDA will relieve her of teaching efforts, giving her time to receive this training and to establish ..
  30. ALKALINE PHOSPHATASE IN THE PREIMPLANTATION MOUSE EMBRYO
    Carol Ziomek; Fiscal Year: 1991
    ..the expression of the embryonic APase will be suppressed by the injection of anti-sense APase RNA into 2-cell blastomeres and by then following the developmental capabilities of these injected cells...
  31. TRANSLATIONAL CONTROL IN C. ELEGANS EMBRYOS
    Craig Hunter; Fiscal Year: 2004
    ..MEX-3 represses pal-1 translation in oocytes and early blastomeres. At the 4-cell stage, this repression is inhibited in the posterior blastomeres and pal-1 is translated in ..
  32. PRODUCTION OF CLONED CALVES FROM ADULT FIBROBLASTS
    GEORGE HOLZER; Fiscal Year: 2000
    ..As controls, in vitro derived blastomeres will serve as nuclear donor cells for nuclear transfer and the establishment of pregnancies...
  33. DETERMINATIVE EVENTS IN CLEAVAGE STAGE EMBRYOS
    Steven Klein; Fiscal Year: 1991
    It is believed that cleavage stage events restrict cell fate because experimental manipulation of blastomeres alters terminal cell differentiation...
  34. CELL ADHESION AND MATRIX IN VERTEBRATE DEVELOPMENT
    Douglas DeSimone; Fiscal Year: 1999
    ..Integrin transcripts will be injected into eggs and specific blastomeres and the adhesion, migration and fate of the progeny determined as development proceeds...
  35. NUCLEAR TRANSFER IN NON-HUMAN PRIMATES
    Steven Voelkel; Fiscal Year: 1991
    ..the most effective means of enucleating oocytes and the extent to which oocytes will fuse with non-primate blastomeres. He plans to obtain oocytes from animals at regional primate facilities that are being sacrificed for other ..
  36. MOLECULAR AND CELLULAR BASIS OF LENS DEVELOPMENT
    Jonathan J Henry; Fiscal Year: 2010
    ....
  37. Spiralian developmental system for the study of multipotency
    Cesar Arenas Mena; Fiscal Year: 2008
    ..The first round of experiments will deplete this histone variant from individual blastomeres of the multipotent terminal growth zone...
  38. MHC-Defined and Identical Twin Monkeys for Research
    Joseph Kemnitz; Fiscal Year: 2005
    ..Additionally, genetically identical macaques will have general utility in biomedical research, especially to the neuroscience community. ..
  39. Role of Par Genes in the Early Mouse Embryo
    VERNADETH ALARCON; Fiscal Year: 2006
    ..An understanding of cell polarization in the mouse embryo should have significant benefit for human health research related to reproductive problems. ..
  40. Specification of embryonic C. elegans mesoderm by a T-box factor
    Morris Maduro; Fiscal Year: 2008
    ..elegans may shed light on how development goes awry, a process which can lead to birth defects or cancer. [unreadable] [unreadable] [unreadable]..
  41. Oxidative Stress and Oocyte Development
    CATHERINE COMBELLES; Fiscal Year: 2007
    ..These findings will enhance our ability to detect and produce oocytes of optimal quality, thereby providing critical foundations for the improvement of female reproductive health. [unreadable] [unreadable] [unreadable]..
  42. REGULATION OF BETA-CATENIN NUCLEARIZATION
    CHARLES ETTENSOHN; Fiscal Year: 2006
    The accumulation of beta-catenin in the nuclei of specific blastomeres is a critical early step in animal development...
  43. IMMUNOLOGIC MECHANISMS IN HUMAN FEMALE INFERTILITY
    John Herr; Fiscal Year: 2002
    ....
  44. A Program for Expanding ELSI Undergraduate Pedagogy
    Ronald Green; Fiscal Year: 2005
    ..This proposal represents a logical next step in the employment of the skills and resources we have developed at Dartmouth for undergraduate ELSI education. ..
  45. BAYLOR BIRCWH PROGRAM
    Joe Simpson; Fiscal Year: 2004
    ..These goals are vital to public health in contributing to the establishment of methods for production of safe, effective, quality herbal extracts for consumer use. ..
  46. TRANSGENIC MODULATION OF GAP JUNCTION INTERACTIONS
    Richard Schultz; Fiscal Year: 2001
    ..1997). Am 5 is to determine whether long term survival of the Cx43 KO mouse may be achieved when Cx43 expression is restored to all neural crest lineages via a Cx43 expression vector driven by the Cx43 promoter. ..
  47. Body Plan Formation in Early Mouse Embryo
    Yusuke Marikawa; Fiscal Year: 2004
    ....
  48. 2003/2005 INTERNATIONAL C. ELEGANS MEETINGS
    Geraldine Seydoux; Fiscal Year: 2004
    ..Previous C. elegans meetings have led to the exchange of knowledge, ideas, methods, mutants, and clones and have been vital in fostering the sense of excitement and the collegiality and cooperativity that characterize this field. ..
  49. Preimplantation Genetic Diagnosis & Genetic Modification
    John Robertson; Fiscal Year: 2003
    ..It will explore whether prevailing conceptions of procreative and family liberty include the right of prospective parents to alter or modify the genome of offspring for medical or non-medical purposes. ..
  50. Mes-1 and Asymmetric Germline Divisions in C. elegans
    LAURA BERKOWITZ; Fiscal Year: 2003
    ..Identification of other genes that function in this process will not only lay the foundation for future experiments but will also advance our understanding of asymmetric divisions. ..
  51. Wnt Signaling in Xenopus Embryonic Development
    Sergei Y Sokol; Fiscal Year: 2010
    ..Since Wnt pathways are activated in many human cancers, including colon carcinomas, melanomas and skin tumors, the proposed experiments are directly relevant to human disease. ..
  52. PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE
    Shoukhrat Mitalipov; Fiscal Year: 2005
    ..The applicants will select the optimal approach to twinning in year 1 and produce 5 sets of identical twins per year during years 2-5 of the study. ..
  53. The Zebrafish Model of Microphthalmia
    Jarema Malicki; Fiscal Year: 2008
    ..These studies will provide insight into the genetic basis of this debilitating disorder that affects 1 in 5,000 children. [unreadable] [unreadable] [unreadable]..
  54. BAC-FISH Assays for Sensitive Karyotyping of Cancer Cells
    Heinz Ulrich Weier; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  55. Impact of Egg Quality on Gene Expression and Behavior
    Richard Schultz; Fiscal Year: 2007
    ..abstract_text> ..
  56. TRANSGENIC MOUSE MODELS OF CRANIOFACIAL DEVELOPMENT
    Richard Behringer; Fiscal Year: 2001
    ..This knowledge should be useful for understanding the etiology and pathogenesis of craniofacial defects. ..
  57. COMPARATIVE GENETICS OF GLI FUNCTION
    Alexander Schier; Fiscal Year: 2008
    ..These comparative genetic studies will allow detailed insights into the conservation and divergence of orthologous genes and will test the value of animal models in understanding human birth defects. ..
  58. NEURONAL LINEAGE DETERMINANTS IN EMBRYOS
    SALLY MOODY; Fiscal Year: 2006
    ..abstract_text> ..
  59. Metastasis-associated kinase in Wnt signaling
    Sergei Y Sokol; Fiscal Year: 2010
    ..The knowledge of the molecular pathways that involve MAK should allow the design of small molecules, which can modulate its enzymatic activity, and may lead to the development of new anti-cancer therapies. ..
  60. Control of cell polarity in the C. elegans zygote
    Geraldine Seydoux; Fiscal Year: 2005
    ..We anticipate that our studies will provide significant insights into these processes by taking advantage of the experimental attributes of a simple model system...
  61. INTERACTION BETWEEN BLASTOCYST AND UTERINE ENDOMETRIUM
    ROBERT MICHAEL ROBERTS; Fiscal Year: 2010
    ..Together these studies are expected to provide information on two key events in early pregnancy, the formation of an early placenta and immediate maternal responses to the presence of a tiny embryo attaching to her uterine wall. ..