retroviridae

Summary

Summary: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).

Top Publications

  1. ncbi LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1
    S Hacein-Bey-Abina
    INSERM Unit 429, Cedex 15, France
    Science 302:415-9. 2003
  2. pmc Hexagonal assembly of a restricting TRIM5alpha protein
    Barbie K Ganser-Pornillos
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 108:534-9. 2011
  3. ncbi Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector
    Andrea L Szymczak
    Nat Biotechnol 22:589-94. 2004
  4. ncbi Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics
    Toshio Kitamura
    Divisions of Cellular Therapy and Hematopoietic Factors, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Exp Hematol 31:1007-14. 2003
  5. pmc GABA-cAMP response element-binding protein signaling regulates maturation and survival of newly generated neurons in the adult hippocampus
    Ravi Jagasia
    Research Group Adult Neural Stem Cells and Neurogenesis, Institute of Developmental Genetics, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, 85764 Munich Neuherberg, Germany
    J Neurosci 29:7966-77. 2009
  6. ncbi Seizure-associated, aberrant neurogenesis in adult rats characterized with retrovirus-mediated cell labeling
    Sebastian Jessberger
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Neurosci 27:9400-7. 2007
  7. pmc LEDGF hybrids efficiently retarget lentiviral integration into heterochromatin
    Rik Gijsbers
    Division of Molecular Medicine, Katholieke Universiteit Leuven, Leuven, Belgium
    Mol Ther 18:552-60. 2010
  8. pmc Retroviral intasome assembly and inhibition of DNA strand transfer
    Stephen Hare
    Division of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Nature 464:232-6. 2010
  9. ncbi Targeting of incoming retroviral Gag to the centrosome involves a direct interaction with the dynein light chain 8
    Coralie Petit
    CNRS UPR9051, Hopital Saint Louis, Conventionné par l Université Paris 7, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France
    J Cell Sci 116:3433-42. 2003
  10. ncbi The APOBEC3 cytidine deaminases: an innate defensive network opposing exogenous retroviruses and endogenous retroelements
    Ya Lin Chiu
    Gladstone Institute of Virology and Immunology, San Francisco, California 94158, USA
    Annu Rev Immunol 26:317-53. 2008

Detail Information

Publications272 found, 100 shown here

  1. ncbi LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1
    S Hacein-Bey-Abina
    INSERM Unit 429, Cedex 15, France
    Science 302:415-9. 2003
    ..Thus, retrovirus vector insertion can trigger deregulated premalignant cell proliferation with unexpected frequency, most likely driven by retrovirus enhancer activity on the LMO2 gene promoter...
  2. pmc Hexagonal assembly of a restricting TRIM5alpha protein
    Barbie K Ganser-Pornillos
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 108:534-9. 2011
    ..Capsid recognition therefore involves a synergistic combination of direct binding interactions, avidity effects, templated assembly, and lattice complementarity...
  3. ncbi Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector
    Andrea L Szymczak
    Nat Biotechnol 22:589-94. 2004
    ....
  4. ncbi Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics
    Toshio Kitamura
    Divisions of Cellular Therapy and Hematopoietic Factors, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Exp Hematol 31:1007-14. 2003
    ..In this review, we describe retrovirus-mediated strategies used for investigation of gene functions and function-based screening strategies...
  5. pmc GABA-cAMP response element-binding protein signaling regulates maturation and survival of newly generated neurons in the adult hippocampus
    Ravi Jagasia
    Research Group Adult Neural Stem Cells and Neurogenesis, Institute of Developmental Genetics, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, 85764 Munich Neuherberg, Germany
    J Neurosci 29:7966-77. 2009
    ..These results indicate that CREB signaling is a central pathway in adult hippocampal neurogenesis, regulating the development and survival of new hippocampal neurons downstream of GABA-mediated excitation...
  6. ncbi Seizure-associated, aberrant neurogenesis in adult rats characterized with retrovirus-mediated cell labeling
    Sebastian Jessberger
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Neurosci 27:9400-7. 2007
    ....
  7. pmc LEDGF hybrids efficiently retarget lentiviral integration into heterochromatin
    Rik Gijsbers
    Division of Molecular Medicine, Katholieke Universiteit Leuven, Leuven, Belgium
    Mol Ther 18:552-60. 2010
    ..Thus, engineered LEDGF/p75 chimeras provide technology for controlling integration site selection by lentiviral vectors...
  8. pmc Retroviral intasome assembly and inhibition of DNA strand transfer
    Stephen Hare
    Division of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Nature 464:232-6. 2010
    ..Our findings define the structural basis of retroviral DNA integration, and will allow modelling of the HIV-1 intasome to aid in the development of antiretroviral drugs...
  9. ncbi Targeting of incoming retroviral Gag to the centrosome involves a direct interaction with the dynein light chain 8
    Coralie Petit
    CNRS UPR9051, Hopital Saint Louis, Conventionné par l Université Paris 7, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France
    J Cell Sci 116:3433-42. 2003
    ..Finally, we describe a direct interaction between Gag and dynein light chain 8 that probably accounts for the specific routing of the incoming capsids to the centrosome prior to nuclear import of the viral genome...
  10. ncbi The APOBEC3 cytidine deaminases: an innate defensive network opposing exogenous retroviruses and endogenous retroelements
    Ya Lin Chiu
    Gladstone Institute of Virology and Immunology, San Francisco, California 94158, USA
    Annu Rev Immunol 26:317-53. 2008
    ....
  11. ncbi Gene therapy for immunodeficiency due to adenosine deaminase deficiency
    Alessandro Aiuti
    San Raffaele Telethon Institute for Gene Therapy, Milan, Italy
    N Engl J Med 360:447-58. 2009
    ..We investigated the long-term outcome of gene therapy for severe combined immunodeficiency (SCID) due to the lack of adenosine deaminase (ADA), a fatal disorder of purine metabolism and immunodeficiency...
  12. ncbi High-definition mapping of retroviral integration sites identifies active regulatory elements in human multipotent hematopoietic progenitors
    Claudia Cattoglio
    Istituto Scientifico H San Raffaele, Milan, Italy
    Blood 116:5507-17. 2010
    ....
  13. pmc Human Trim5α has additional activities that are uncoupled from retroviral capsid recognition
    Semih U Tareen
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA
    Virology 409:113-20. 2011
    ..Our results demonstrate functions of Trim5α that are not dependent on recognizing the retroviral capsid...
  14. ncbi Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes
    Trond Aasen
    Center of Regenerative Medicine in Barcelona, Dr Aiguader 88, 08003 Barcelona, Spain
    Nat Biotechnol 26:1276-84. 2008
    ..Our findings provide an experimental model for investigating the bases of cellular reprogramming and highlight potential advantages of using keratinocytes to generate patient-specific iPS cells...
  15. ncbi Correction of ADA-SCID by stem cell gene therapy combined with nonmyeloablative conditioning
    Alessandro Aiuti
    San Raffaele Telethon Institute for Gene Therapy HSR TIGET, Milan, Italy
    Science 296:2410-3. 2002
    ..Both patients are currently at home and clinically well, with normal growth and development. These results indicate the safety and efficacy of HSC gene therapy combined with nonmyeloablative conditioning for the treatment of SCID...
  16. pmc Detecting statistically significant common insertion sites in retroviral insertional mutagenesis screens
    Jeroen de Ridder
    Information and Communication Theory Group, Faculty of Electrical Engineering, Mathematics, and Computer Science, Delft University of Technology, Delft, The Netherlands
    PLoS Comput Biol 2:e166. 2006
    ..Still, with the FWE under control, application of our method resulted in the discovery of eight novel CISs, which each have a probability less than 5% of being false detections...
  17. pmc Restriction of multiple divergent retroviruses by Lv1 and Ref1
    Theodora Hatziioannou
    Aaron Diamond AIDS Research Center and The Rockefeller University, 455 First Avenue, New York, NY 10016, USA
    EMBO J 22:385-94. 2003
    ..These data suggest that restriction factors in human and non-human primate cells are able to recognize and block infection by multiple, widely divergent retroviruses and that the factors themselves may be related...
  18. pmc Integration profile of retroviral vector in gene therapy treated patients is cell-specific according to gene expression and chromatin conformation of target cell
    Luca Biasco
    San Raffaele Telethon Institute for Gene Therapy HSR TIGET, Milano, Italy
    EMBO Mol Med 3:89-101. 2011
    ..Our study shows that MLV vector insertional profile is cell-specific according to the genetic/chromatin state of the target cell both in vitro and in vivo in patients several years after GT...
  19. pmc Multiple integrated copies and high-level production of the human retrovirus XMRV (xenotropic murine leukemia virus-related virus) from 22Rv1 prostate carcinoma cells
    Emily C Knouf
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    J Virol 83:7353-6. 2009
    ....
  20. pmc Single-chain VαVβ T-cell receptors function without mispairing with endogenous TCR chains
    D H Aggen
    Department of Biochemistry, University of Illinois, Urbana, IL 61801, USA
    Gene Ther 19:365-74. 2012
    ..Such transduced T cells can respond to the targeted antigen independent of CD3 subunits via the introduced scTv, without the transduced T cells acquiring any new undefined and potentially dangerous specificities...
  21. ncbi Efficiency of T-cell receptor expression in dual-specific T cells is controlled by the intrinsic qualities of the TCR chains within the TCR-CD3 complex
    Mirjam H M Heemskerk
    Department of Hematology, Leiden University Medical Center, C2 R, PO Box 9600, 2300 RC Leiden, The Netherlands
    Blood 109:235-43. 2007
    ..In conclusion, introduced, endogenous, and chimeric TCRs compete for cell-surface expression in favor of the TCR-CD3 complex with best-pairing properties...
  22. ncbi Retroviruses and the placenta
    David Haig
    Department of Organismic and Evolutionary Biology, Harvard University, Cambridge MA 02138, USA
    Curr Biol 22:R609-13. 2012
    ..The evolutionary interplay between retroviruses and host defenses may have contributed to the remarkable diversity of form among mammalian placentas and to mechanisms of genomic imprinting...
  23. ncbi Identification of cooperative genes for NUP98-HOXA9 in myeloid leukemogenesis using a mouse model
    Masayuki Iwasaki
    Department of Carcinogenesis, Japanese Foundation for Cancer Research, Tokyo, Japan
    Blood 105:784-93. 2005
    ..These genes cooperated with NUP98-HOXA9 in transforming NIH 3T3 cells. The system described here is a powerful tool to identify cooperative oncogenes and will assist in the clarification of the multistep process of carcinogenesis...
  24. pmc Contribution of SAM and HD domains to retroviral restriction mediated by human SAMHD1
    Tommy E White
    Department of Microbiology and Immunology, Albert Einstein College of Medicine Bronx, NY 10461, USA
    Virology 436:81-90. 2013
    ....
  25. pmc Host restriction factors blocking retroviral replication
    Daniel Wolf
    HHMI, Department of Biochemistry, Columbia University, New York, NY 10032, USA
    Annu Rev Genet 42:143-63. 2008
    ..This review is a survey of the best-characterized restriction factors capable of inhibiting retroviral replication and aims to highlight the diversity of strategies used for this task...
  26. ncbi A sensitive retroviral pseudotype assay for influenza H5N1-neutralizing antibodies
    Nigel J Temperton
    MRC UCL Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, 46 Cleveland Street, London, United Kingdom
    Influenza Other Respir Viruses 1:105-12. 2007
    ..We have used retroviral pseudotypes bearing influenza H5 hemagglutinin (HA) as safe, surrogate viruses for influenza neutralization assays which can be carried out at Biosafety Level 2...
  27. ncbi The role of apoptosis in creating and maintaining luminal space within normal and oncogene-expressing mammary acini
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 111:29-40. 2002
    ..Hence, apoptosis can counteract increased proliferation to maintain luminal space, suggesting that tumor cells must restrain apoptosis to populate the lumen...
  28. ncbi Retroviral pseudotransduction for targeted cell manipulation
    Melanie Galla
    Division of Experimental Hematology, Cincinnati Children s Hospital Research Foundation, Cincinnati, OH 45229, USA
    Mol Cell 16:309-15. 2004
    ..Thus, retrovirally delivered mRNA may serve as an immediate translation template if not being reverse transcribed. This approach allows multiple modifications for targeted and reversible cell manipulation with nucleic acids...
  29. pmc A versatile approach to multiple gene RNA interference using microRNA-based short hairpin RNAs
    Xiaocui Zhu
    The Alliance for Cellular Signaling, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    BMC Mol Biol 8:98. 2007
    ..Effective and stable knockdown of multiple gene targets by RNA interference is often necessary to overcome isoform redundancy, but it remains a technical challenge when working with intractable cell systems...
  30. pmc Identification of two intracellular mechanisms leading to reduced expression of oncoretrovirus envelope glycoproteins at the cell surface
    M P Grange
    INSERM U332, Institut Cochin de Genetique Moleculaire, 75014 Paris, France
    J Virol 74:11734-43. 2000
    ..These results indicate that retroviruses have evolved two distinct mechanisms to ensure a similar biological feature: low concentrations of their glycoproteins at the cell surface...
  31. ncbi Improved expression and reactivity of transduced tumor-specific TCRs in human lymphocytes by specific silencing of endogenous TCR
    Sachiko Okamoto
    Center for Cell and Gene Therapy, Takara Bio, Inc, Shiga, Japan
    Cancer Res 69:9003-11. 2009
    ..In consequence, the vector-transduced lymphocytes showed enhanced cytotoxic activity against antigen-expressing tumor cells. Therefore, our novel TCR gene therapy may open a new gate for effective immunotherapy in cancer patients...
  32. pmc MET and MYC cooperate in mammary tumorigenesis
    Alana L Welm
    G W Hooper Research Foundation and Department of Microbiology and Immunology, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 102:4324-9. 2005
    ..The approach described here should provide a useful model with which to efficiently test effects of various genes on tumor development in the breast...
  33. ncbi Human T-lymphocyte cytotoxicity and proliferation directed by a single chimeric TCRzeta /CD28 receptor
    John Maher
    Department of Human Genetics Medicine, Gene Transfer and Somatic Cell Engineering Laboratory, and Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Nat Biotechnol 20:70-5. 2002
    ..These findings have important implications for adoptive immunotherapy of cancer, especially in the context of tumor cells that fail to express major histocompatibility complex antigens and co-stimulatory molecules...
  34. pmc Localization of a retroviral element within the rd gene coding for the beta subunit of cGMP phosphodiesterase
    C Bowes
    Jules Stein Eye Institute, University of California School of Medicine, Los Angeles 90024
    Proc Natl Acad Sci U S A 90:2955-9. 1993
    ..Reverse transcription-PCR demonstrates that the integrated Xmv-28 sequences are transcribed in the retina. The provirus is present in every strain of rd mouse tested...
  35. ncbi Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
    ..These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required...
  36. pmc AML1-ETO inhibits maturation of multiple lymphohematopoietic lineages and induces myeloblast transformation in synergy with ICSBP deficiency
    Maike Schwieger
    Department of Cell and Virus Genetics, Heinrich Pette Institut für Experimentelle Virologie und Immunologie, D 20251 Hamburg, Germany
    J Exp Med 196:1227-40. 2002
    ..Our findings demonstrate that AML1-ETO synergizes with an ICSBP deficiency to induce myeloblastic transformation in the BM, reminiscent of AML...
  37. pmc Deciphering the code for retroviral integration target site selection
    Federico Andrea Santoni
    Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland
    PLoS Comput Biol 6:e1001008. 2010
    ....
  38. pmc Charged polymers modulate retrovirus transduction via membrane charge neutralization and virus aggregation
    Howard E Davis
    Center for Engineering in Medicine Surgical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Biophys J 86:1234-42. 2004
    ....
  39. ncbi SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells
    I Chueh Huang
    Pulmonary Division, Children s Hospital, Children s Hospital Laboratory of Molecular Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:3198-203. 2006
    ..Our data indicate that two coronaviruses that utilize a common receptor nonetheless enter cells through distinct mechanisms...
  40. pmc Receptors and entry cofactors for retroviruses include single and multiple transmembrane-spanning proteins as well as newly described glycophosphatidylinositol-anchored and secreted proteins
    J Overbaugh
    Division of Human Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave, Seattle, WA 98109 1024, USA
    Microbiol Mol Biol Rev 65:371-89, table of contents. 2001
    ..In this review, we will focus on the various strategies used by mammalian retroviruses to gain entry into the cell. The choice of receptors will also be discussed in light of pressures that drive viral evolution and persistence...
  41. pmc Dynamics of gene-modified progenitor cells analyzed by tracking retroviral integration sites in a human SCID-X1 gene therapy trial
    Gary P Wang
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6076, USA
    Blood 115:4356-66. 2010
    ..The approaches to sequencing and bioinformatics analysis reported here should be widely useful in assessing the outcome of gene therapy trials...
  42. ncbi TRIM family proteins: retroviral restriction and antiviral defence
    Sebastien Nisole
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7151, Universite Paris 7, Hopital Saint Louis, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France
    Nat Rev Microbiol 3:799-808. 2005
    ..These findings are reviewed here, and the possibility that TRIMs represent a new and widespread class of antiviral proteins involved in innate immunity is also considered...
  43. pmc Expression pattern of the transcription factor Olig2 in response to brain injuries: implications for neuronal repair
    Annalisa Buffo
    Institute for Stem Cell Research, National Research Center for Environment and Health, Neuherberg Munich, Germany
    Proc Natl Acad Sci U S A 102:18183-8. 2005
    ..These data, therefore, imply Olig2 as a repressor of neurogenesis in cells reacting to brain injury and open innovative perspectives toward evoking endogenous neuronal repair...
  44. pmc Use of endogenous retroviral sequences (ERVs) and structural markers for retroviral phylogenetic inference and taxonomy
    Patric Jern
    Department of Medical Sciences, Uppsala University, Uppsala, Sweden
    Retrovirology 2:50. 2005
    ..The aim was to find traits useful for classification and evolutionary studies of retroviruses. Some of these traits have been used by others, but they are here tested in a wider context than before...
  45. pmc Restriction by APOBEC3 proteins of endogenous retroviruses with an extracellular life cycle: ex vivo effects and in vivo "traces" on the murine IAPE and human HERV-K elements
    Cécile Esnault
    Unité des rétrovirus endogènes et éléments rétroïdes des eucaryotes supérieurs, CNRS UMR 8122, Institut Gustave Roussy, 39 rue Camille Desmoulins, F 94805 Villejuif, and Université Paris Sud, Orsay, F 91405, France
    Retrovirology 5:75. 2008
    ..In addition, APOBEC3 proteins can severely restrict the intracellular transposition of a series of retroelements with a strictly intracellular life cycle, including the murine IAP and MusD LTR-retrotransposons...
  46. ncbi A large number of the human microRNAs target lentiviruses, retroviruses, and endogenous retroviruses
    Shazia T Hakim
    Department of Microbiology, Jinnah University for Women, Karachi 74600, Pakistan
    Biochem Biophys Res Commun 369:357-62. 2008
    ..e. HERV-K). Implications of these observations in RE disease and therapy are discussed...
  47. ncbi Enhanced functionality of T cell receptor-redirected T cells is defined by the transgene cassette
    Matthias Leisegang
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13092, Berlin, Germany
    J Mol Med (Berl) 86:573-83. 2008
    ..Differences in expression were independent of copy number integration as shown by real-time PCR. Thus, linking TCRalpha- and beta-chain genes by a 2A peptide is superior to an IRES for TCR expression and T cell function...
  48. ncbi Improving transcriptional termination of self-inactivating gamma-retroviral and lentiviral vectors
    Axel Schambach
    1Department of Experimental Hematology, Hannover Medical School, Hannover, Germany
    Mol Ther 15:1167-73. 2007
    ..Importantly, the 2xSV USE was superior to the wPRE in suppressing transcriptional read-through, improving not only vector efficiency but potentially also biosafety...
  49. ncbi Transfer of mRNA encoding recombinant immunoreceptors reprograms CD4+ and CD8+ T cells for use in the adoptive immunotherapy of cancer
    K Birkholz
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Gene Ther 16:596-604. 2009
    ..RNA electroporation of T cells thereby provides a versatile tool for transient immunoreceptor expression, which may be of advantage in avoiding the persistence of unintended autoaggression...
  50. pmc TRIM28 mediates primer binding site-targeted silencing of Lys1,2 tRNA-utilizing retroviruses in embryonic cells
    Daniel Wolf
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 105:12521-6. 2008
    ..These results generalize the role of TRIM28 in retroviral restriction and suggest that this system has evolved to restrict multiple retroviruses...
  51. ncbi Growth factor treatment and genetic manipulation stimulate neurogenesis and oligodendrogenesis by endogenous neural progenitors in the injured adult spinal cord
    Yasuo Ohori
    Division of Developmental Biology, Cincinnati Children s Hospital Research Foundation, Cincinnati, Ohio 45229 3039, USA
    J Neurosci 26:11948-60. 2006
    ..These results demonstrate that endogenous neural progenitors can be manipulated to replace neurons and oligodendrocytes lost to insults in the injured spinal cord...
  52. pmc Relationships of gag-pol diversity between Ty3/Gypsy and Retroviridae LTR retroelements and the three kings hypothesis
    Carlos Llorens
    Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Polígono de coma S N, Paterna, Valencia, Spain
    BMC Evol Biol 8:276. 2008
    The origin of vertebrate retroviruses (Retroviridae) is yet to be thoroughly investigated, but due to their similarity and identical gag-pol (and env) genome structure, it is accepted that they evolve from Ty3/Gypsy LTR retroelements the ..
  53. pmc Modulation of retroviral restriction and proteasome inhibitor-resistant turnover by changes in the TRIM5alpha B-box 2 domain
    Felipe Diaz-Griffero
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    J Virol 81:10362-78. 2007
    ..However, testing of an array of mutants revealed that the rapid turnover and retroviral restriction functions of this B-box 2 region are separable...
  54. pmc Absence of xenotropic murine leukaemia virus-related virus in UK patients with chronic fatigue syndrome
    Harriet C T Groom
    Division of Virology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Retrovirology 7:10. 2010
    ....
  55. pmc Directing astroglia from the cerebral cortex into subtype specific functional neurons
    Christophe Heinrich
    Department of Physiological Genomics, Institute of Physiology, Ludwig Maximilians University Munich, Munich, Germany
    PLoS Biol 8:e1000373. 2010
    ..Moreover, neuronal reprogramming of astroglia is not restricted to postnatal stages but can also be achieved from terminally differentiated astroglia of the adult cerebral cortex following injury-induced reactivation...
  56. pmc Immunotherapy of murine retrovirus-induced acquired immunodeficiency by CD4 T regulatory cell depletion and PD-1 blockade
    Wen Li
    Department of Microbiology and Immunology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
    J Virol 85:13342-53. 2011
    ....
  57. pmc The use of chromatin insulators to improve the expression and safety of integrating gene transfer vectors
    David W Emery
    University of Washington Department of Medicine, Division of Medical Genetics, and Institute for Stem Cell and Regenerative Medicine, Seattle, WA 98109, USA
    Hum Gene Ther 22:761-74. 2011
    ....
  58. pmc The programmed death-1 and interleukin-10 pathways play a down-modulatory role in LP-BM5 retrovirus-induced murine immunodeficiency syndrome
    Kathy A Green
    Department of Microbiology and Immunology, Dartmouth Medical School, 1 Medical Center Drive, Lebanon, NH 03756, USA
    J Virol 82:2456-69. 2008
    ..Thus, it appears that PD-1/PD-L interactions and IL-10 are primarily important in moderating the effects of LP-BM5-induced disease in B6 mice...
  59. ncbi Advances in viral-vector systemic cytokine gene therapy against cancer
    Lihua Liu
    Research Center, the Fourth Clinical Hospital of Hebei Medical University and Hebei Cancer Institute, 12 Jiankanglu, Shijiazhuang, 050011, China
    Vaccine 28:3883-7. 2010
    ..Additionally, we will also discuss and propose future perspectives about cancer gene therapy...
  60. pmc Jaagsiekte sheep retrovirus utilizes a pH-dependent endocytosis pathway for entry
    Pascale Bertrand
    Department of Microbiology and Immunology, McGill University, Montreal, Canada
    J Virol 82:2555-9. 2008
    ..Altogether, this study reveals that JSRV utilizes a pH-dependent, dynamin-associated endocytosis pathway for entry that differs from the classical pH-dependent entry pathway of vesicular stomatitis virus...
  61. ncbi Transiently redirected T cells for adoptive transfer
    Hilde Almåsbak
    Section for Immunology, Radiumhospitalet, Oslo University Hospital, Oslo, Norway
    Cytotherapy 13:629-40. 2011
    ..We demonstrate that transfection of T cells with messenger (m) RNA coding for CAR is an alternative strategy...
  62. ncbi Retroviral vectors for high-level transgene expression in T lymphocytes
    Boris Engels
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13092 Berlin, Germany
    Hum Gene Ther 14:1155-68. 2003
    ..In summary, MP71 vectors are useful tools for stable, high-level gene expression in T lymphocytes, for example, in the expression of T cell receptor genes...
  63. ncbi Transcription of two human genes from a bidirectional endogenous retrovirus promoter
    Catherine A Dunn
    Terry Fox Laboratory, BC Cancer Agency, 675 West 10th Avenue, Vancouver, BC, Canada V5Z 1L3
    Gene 366:335-42. 2006
    ..Finally, we show that the ERV LTR also exists in the genomes of several non-human primates, and present evidence that potential transcription factor binding sites in the core region have been maintained throughout primate evolution...
  64. ncbi Replication-competent vectors and empty virus-like particles: new retroviral vector designs for cancer gene therapy or vaccines
    Charlotte Dalba
    Biologie et Thérapeutique des Pathologies Immunitaires, CNRS, Université Pierre et Marie Curie Paris, UMR 7087, Paris, France
    Mol Ther 15:457-66. 2007
    ....
  65. pmc Disrupted-In-Schizophrenia 1 regulates integration of newly generated neurons in the adult brain
    Xin Duan
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, 733 N Broadway, Baltimore, MD 21205, USA
    Cell 130:1146-58. 2007
    ....
  66. pmc Targeting of murine leukemia virus gag to the plasma membrane is mediated by PI(4,5)P2/PS and a polybasic region in the matrix
    E Hamard-Peron
    LaboRetro, INSERM U758, ENS de Lyon, IFR128, 69364 Lyon, France
    J Virol 84:503-15. 2010
    ....
  67. pmc Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery
    Melanie Galla
    Department of Experimental Hematology, Institute of Transplantation Immunology, Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, Germany
    Nucleic Acids Res 39:7147-60. 2011
    ..Transposase-mediated gene transfer can thus be tuned to maintain high efficiency in the absence of overt cell damage...
  68. pmc Cross-clade inhibition of HIV-1 replication and cytopathology by using RNase P-associated external guide sequences
    Gunter Kraus
    Department of Microbiology, University of Miami, 1550 NW 10th Avenue R 138, Miami, FL 33136, USA
    Proc Natl Acad Sci U S A 99:3406-11. 2002
    ..Viral replication and cytopathology associated with infection of multiple HIV-1 clades can be effectively inhibited in CD4(+) cells expressing the RNase P-associated U5 EGS (560) molecule...
  69. pmc Fusogenicity of Jaagsiekte sheep retrovirus envelope protein is dependent on low pH and is enhanced by cytoplasmic tail truncations
    Marceline Côté
    McGill University, Department of Microbiology and Immunology, 3775 University St, Montreal, QC H3A 2B4, Canada
    J Virol 82:2543-54. 2008
    ....
  70. ncbi The position of the AUG start codon in MFG-based γ-retroviral vectors has a dramatic effect on translation-dependent protein expression
    Timothy L Frankel
    Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    J Gene Med 13:478-86. 2011
    ..Little is known about the impact of variations in start codon location within MFG-based vectors on protein expression...
  71. ncbi Poised for contagion: evolutionary origins of the infectious abilities of invertebrate retroviruses
    H S Malik
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 USA
    Genome Res 10:1307-18. 2000
    ..This has implications for the evolutionary history of retroviruses as well as for the potential ability of all LTR-retrotransposable elements to become infectious agents...
  72. pmc The MN1-TEL fusion protein, encoded by the translocation (12;22)(p13;q11) in myeloid leukemia, is a transcription factor with transforming activity
    A Buijs
    Department of Genetics, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Mol Cell Biol 20:9281-93. 2000
    ..The transactivating capacity of MN1-TEL depended on both the DBD of TEL and sequences in MN1. MN1-TEL contributes to leukemogenesis by a mechanism distinct from that of other chimeric proteins containing TEL...
  73. pmc Rational optimization of reprogramming culture conditions for the generation of induced pluripotent stem cells with ultra-high efficiency and fast kinetics
    Jiekai Chen
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine at Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Cell Res 21:884-94. 2011
    ..This new paradigm not only provides a rationale to further improve iPSC generation but also simplifies the conceptual understanding of reprogramming by defined factors...
  74. ncbi Synthetically functionalized retroviruses produced from the bioorthogonally engineered cell surface
    Shirley Wong
    Medicinal Chemistry and Pharmacology Program, University of California, Irvine, California 92697, United States
    Bioconjug Chem 22:151-5. 2011
    ....
  75. pmc Genotoxicity of retroviral hematopoietic stem cell gene therapy
    Grant D Trobridge
    Washington State University, Department of Pharmaceutical Sciences and School of Molecular Biosciences, P O Box 646534, Pullman, WA 99164 6534, USA
    Expert Opin Biol Ther 11:581-93. 2011
    ..These genotoxicity studies are critical to advancing HSC gene therapy...
  76. ncbi Mutation of CpGs in the murine stem cell virus retroviral vector long terminal repeat represses silencing in embryonic stem cells
    C Scott Swindle
    Department of Microbiology, Division of Development and Clinical Immunology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 279:34-41. 2004
    ..Furthermore, distinct mechanisms are suggested for establishment and maintenance of the silenced proviral state...
  77. ncbi Primary polyclonal human T lymphocytes targeted to carcino-embryonic antigens and neural cell adhesion molecule tumor antigens by CD3zeta-based chimeric immune receptors
    David E Gilham
    CRC Department of Medical Oncology, Paterson Institute for Cancer Research, University of Manchester, UK
    J Immunother 25:139-51. 2002
    ..Viability was improved by a costimulatory signal indicating that such signals may be vital in the maintenance of long-term functional activity of receptor modified T lymphocytes...
  78. ncbi TCR reconstitution in Jurkat reporter cells facilitates the identification of novel tumor antigens by cDNA expression cloning
    Corlien A Aarnoudse
    Department of Clinical Oncology, Leiden University Medical Center, Leiden, The Netherlands
    Int J Cancer 99:7-13. 2002
    ..Our results illustrate that TCR-reconstituted Jurkat reporter cells are a useful tool in the identification of novel tumor antigens by cDNA expression cloning...
  79. pmc Retroviral DNA integration: ASLV, HIV, and MLV show distinct target site preferences
    Rick S Mitchell
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    PLoS Biol 2:E234. 2004
    ..Thus, each of the three retroviruses studied showed unique integration site preferences, suggesting that virus-specific binding of integration complexes to chromatin features likely guides site selection...
  80. ncbi Identification of candidate cooperative genes of the Apc mutation in transformation of the colon epithelial cell by retroviral insertional mutagenesis
    Miwa Tanaka
    Department of Carcinogenesis, The Cancer Institute, Japanese Foundation for Cancer Research, 3 10 6 Ariake, Koto ku, Tokyo 135 8550, Japan
    Cancer Sci 99:979-85. 2008
    ..These data suggest the importance of cytoskeletal function in Apc-related tumor development and the usefulness of RIM in non-hematopoietic tissues, providing new insight into the early stage of colon carcinogenesis...
  81. pmc Recent advances in lentiviral vector development and applications
    Janka Matrai
    Flanders Institute for Biotechnology, VIB, Vesalius Research Center, University of Leuven, Leuven, Belgium
    Mol Ther 18:477-90. 2010
    ..These recent advances underscore the improved safety and efficacy of LVs with important implications for clinical trials...
  82. pmc Network dynamics of eukaryotic LTR retroelements beyond phylogenetic trees
    Carlos Llorens
    Institut Cavanilles de Biodiversitat i Biologia Evolutiva ICBiBE, Universitat de Valencia, Paterna, Valencia, Spain
    Biol Direct 4:41. 2009
    ..viruses evolve from LTR retrotransposons and that this whole system clusters into five families: Ty3/Gypsy, Retroviridae, Ty1/Copia, Bel/Pao and Caulimoviridae...
  83. pmc Cell-culture assays reveal the importance of retroviral vector design for insertional genotoxicity
    Ute Modlich
    Department of Experimental Hematology, Hannover Medical School, Carl Neuberg Strasse 1, D 30625 Hannover, Germany
    Blood 108:2545-53. 2006
    ..Additional modifications of SIN vectors may further increase safety. Improved cell-culture assays will likely play an important role in the evaluation of insertional mutagenesis...
  84. pmc Hare TRIM5α restricts divergent retroviruses and exhibits significant sequence variation from closely related lagomorpha TRIM5 genes
    Adam J Fletcher
    MRC Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, Windeyer Building, London, United Kingdom
    J Virol 84:12463-8. 2010
    ..We propose that, like primates, lagomorphs have been subject to selective pressure from TRIM5-sensitive viruses, possibly related to the endogenous lentivirus RELIK found in both rabbits and hares...
  85. pmc T cell receptor gene therapy for cancer
    Thomas M Schmitt
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Hum Gene Ther 20:1240-8. 2009
    ..Here we outline the challenges of TCR gene therapy and the advances that have been made toward realizing the promise of this approach...
  86. pmc Advanced phase I/II studies of targeted gene delivery in vivo: intravenous Rexin-G for gemcitabine-resistant metastatic pancreatic cancer
    Sant P Chawla
    Sarcoma Oncology Center, Santa Monica, California, USA
    Mol Ther 18:435-41. 2010
    ....
  87. ncbi Integration of retroviral vectors induces minor changes in the transcriptional activity of T cells from ADA-SCID patients treated with gene therapy
    Barbara Cassani
    San Raffaele Telethon Institute for Gene Therapy HSR TIGET, Milan, Italy
    Blood 114:3546-56. 2009
    ..The trials described herein have been registered at http://www.clinicaltrials.gov as #NCT00598481 and #NCT00599781...
  88. ncbi E-cadherin contributes to the bystander effect of TK/GCV suicide therapy and enhances its antitumoral activity in pancreatic cancer models
    L García-Rodriguez
    Programa Gens i Malaltia, Centre de Regulacio Genomica, Parc de Recerca Biomedica de Barcelona, UPF, Barcelona, Spain
    Gene Ther 18:73-81. 2011
    ..Thus, we propose that by restoring E-cadherin in pancreatic tumor cells we will improve TK/GCV therapy, both by enhancing the bystander effect and by facilitating the induction of apoptosis...
  89. ncbi Gene therapy of thyroid cancer via retrovirally-driven combined expression of human interleukin-2 and herpes simplex virus thymidine kinase
    Luisa Barzon
    Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Via Gabelli 63, I 35121 Padova, Italy
    Eur J Endocrinol 148:73-80. 2003
    ..e. thymidine kinase of herpes simplex virus, HSV-TK, and interleukin-2, IL-2) and evaluated its efficiency in vitro and in vivo...
  90. ncbi PDGF stimulates the massive expansion of glial progenitors in the neonatal forebrain
    M C Assanah
    Department of Neurological Surgery, Columbia University, New York, New York 10032, USA
    Glia 57:1835-47. 2009
    ..These findings reveal the inherent growth factor responsiveness and tumorigenic potential of PDGFRalpha+ progenitors and highlight the importance of paracrine signaling in stimulating glioma growth and infiltration...
  91. pmc Phase I/II and phase II studies of targeted gene delivery in vivo: intravenous Rexin-G for chemotherapy-resistant sarcoma and osteosarcoma
    Sant P Chawla
    The Sarcoma Oncology Center, Santa Monica, California, USA
    Mol Ther 17:1651-7. 2009
    ..9 months. These studies suggest that Rexin-G is safe, may help control tumor growth, and may possibly improve survival in chemotherapy-resistant sarcoma and osteosarcoma...
  92. pmc Transcriptional enhancers induce insertional gene deregulation independently from the vector type and design
    Giulietta Maruggi
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, Modena, Italy
    Mol Ther 17:851-6. 2009
    ..This study indicates that insertional gene activation is determined by the characteristics of the transcriptional regulatory elements carried by the vector, and is largely independent from the vector type or design...
  93. pmc An expanded clade of rodent Trim5 genes
    Semih U Tareen
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA
    Virology 385:473-83. 2009
    ..Sampling six rodent Trim5 genes failed to reveal antiviral activities against a set of eight retroviral challenges, although we predict that such activities exist...
  94. ncbi Glial progenitors in adult white matter are driven to form malignant gliomas by platelet-derived growth factor-expressing retroviruses
    Marcela Assanah
    Department of Pathology, Columbia University, New York, New York 10032, USA
    J Neurosci 26:6781-90. 2006
    ....
  95. pmc Retina regeneration in the chick embryo is not induced by spontaneous Mitf downregulation but requires FGF/FGFR/MEK/Erk dependent upregulation of Pax6
    Jason R Spence
    Department of Zoology, Miami University, Oxford, Ohio 45056, USA
    Mol Vis 13:57-65. 2007
    ..To elucidate the early cellular events that take place during induction of retina regeneration in the embryonic chick, focusing on the relationship between fibroblast growth factor (FGF) signaling and the regulation of Pax6 and Mitf...
  96. pmc RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells
    Y Ishida
    Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, 200 Longwood Avenue, Boston, MA 02115, USA
    Nucleic Acids Res 27:e35. 1999
    ....
  97. ncbi Functional association of cyclophilin A with HIV-1 virions
    M Thali
    Division of Human Retrovirology, Dana Farber Cancer Institute, Boston, Massachusetts
    Nature 372:363-5. 1994
    ..Moreover, SDZ NIM811 inhibited the replication of HIV-1 but was inactive against SIVMAC, a primate immunodeficiency virus closely related to HIV-1, which does not incorporate cyclophilin A...
  98. ncbi Overexpression of the myeloid leukemia-associated Hoxa9 gene in bone marrow cells induces stem cell expansion
    Unnur Thorsteinsdottir
    Laboratory of Molecular Genetics of Hemopoietic Stem Cells, Clinical Research Institute of Montreal, Quebec, Canada
    Blood 99:121-9. 2002
    ..Interestingly, despite specifically directing high expression of Hoxa9 in T and B lymphoid lineages, none of the Hoxa9 transgenic mice developed lymphoid malignancies for the observation period of more than 18 months...
  99. pmc RANK is the intrinsic hematopoietic cell surface receptor that controls osteoclastogenesis and regulation of bone mass and calcium metabolism
    J Li
    Departments of Cell Biology, Amgen Inc, One Amgen Center Drive, Thousand Oaks, CA 91320, USA
    Proc Natl Acad Sci U S A 97:1566-71. 2000
    ....
  100. ncbi Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy
    Ryosuke Uchibori
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan
    J Gene Med 11:373-81. 2009
    ..For efficient therapeutic application, we developed retroviral vector-producing MSCs that enhance tumor transduction via progeny vector production...
  101. pmc Similar MLL-associated leukemias arising from self-renewing stem cells and short-lived myeloid progenitors
    Antonio Cozzio
    Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Genes Dev 17:3029-35. 2003
    ....

Research Grants76

  1. Characterization of the antiviral and nuclear regulatory functions of FEZ1 &NEK1
    MOJGAN HOSSEINI NAGHAVI; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Human immunodeficiency virus type 1 (HIV-1) is a member of the retroviridae that causes Acquired Immunodeficiency Syndome (AIDS), which affects over 33.3 million people worldwide...
  2. Upregulation of ZAP expression as a novel approach for anti-alphavirus therapy
    Margaret R Macdonald; Fiscal Year: 2013
    ..mediates potent inhibition of alphaviruses, including the prototype Sindbis virus, as well as viruses in the Retroviridae and Filoviridae families...
  3. Genome wide siRNA screen for identifying host factors required for ZAP function
    Margaret R Macdonald; Fiscal Year: 2013
    ..ZAP) is a host protein that demonstrates potent inhibition of viruses in this genus, as well as viruses in the Retroviridae and Filoviridae families...
  4. STRUCTURE AND ASSEMBLY OF THE HIV1 CAPSID PROTEIN
    Christopher Hill; Fiscal Year: 2001
    ..it contains a 20 amino acid stretch, termed the major homology region (MHR), which is highly conserved across retroviridae 3-5...
  5. CONSTRUCTION AND ANALYSIS OF RETROVIRUS MUTANTS
    Stephen Goff; Fiscal Year: 2003
    ..In each case, these tests will be applied to existing panels of mutants to help localize the regions needed for these activities. ..
  6. OVERCOMING POSITION EFFECTS WITH CHROMATIN INSULATORS
    David Emery; Fiscal Year: 2008
    ..abstract_text> ..
  7. Molecular Therapy Core Centers
    Arthur Miller; Fiscal Year: 2008
    ..abstract_text> ..
  8. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
  9. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
  10. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  11. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    ..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
  12. Lanthanides as Novel Anti-Arthritic Agents
    Christopher Evans; Fiscal Year: 2004
    ..A successful outcome to these studies will have identified the basis for a potential new, very inexpensive anti-rheumatic agent whose mode of action differs from that of existing drugs. ..
  13. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  14. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
    ..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. [unreadable] [unreadable] [unreadable]..
  15. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  16. Clinical Trial Planning Grant: Lentiviral Vector for HIV-1
    Donald Kohn; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  17. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  18. Novel Nanostructured Calcium Phosphate for Gene Delivery to Bone
    Christopher Evans; Fiscal Year: 2005
    ..2. To evaluate the ability of nCaP-vector constructs to enhance osteogenic responses in vivo. ..
  19. NEURODEGENERATIVE MECHANISMS IN ALZHEIMER'S DISEASE
    Piruz Nahreini; Fiscal Year: 2004
    ....
  20. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
  21. AAOS/NIH Mulit-Year Research Symposia for 2005-2009
    Christopher Evans; Fiscal Year: 2005
    ....
  22. Conference--American Society of Gene Therapy
    Donald Kohn; Fiscal Year: 2008
    ..The annual ASGT meeting is the premier meeting in the field of gene therapy and serves as a rich source of information for the future generation of gene therapists. ..
  23. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  24. Delivery of Therapeutic Genes to Osteoarthritic Joints
    Christopher Evans; Fiscal Year: 2002
    ....
  25. GENE EXPRESSION IN BETA-CELLS BY LENTIVIRAL VECTORS
    Donald Kohn; Fiscal Year: 2003
    ..g. beta-cell precursors), or as reporter genes to indicate the transcriptional status of the insulin or PDX-1 genes, e.g. in response to glucose concentrations, hormones, growth factors, genes, or drugs. ..
  26. Gene Therapy for hemophilia using muscle-expressed FVIIa
    Katherine A High; Fiscal Year: 2011
    ..The proposed work will allow us to increase the therapeutic applicability of our approach and enhance its safety, prior to a clinical application. ..
  27. GFP-Based Assays to Probe Transcriptional Controls(RMI)
    Richard Katz; Fiscal Year: 2005
    ..The assays have the potential to reveal new lead compounds, as well as new gene targets for therapeutics. ..
  28. Translational Control of Retroviral Unspliced mRNA
    KATHLEEN A BORIS LAWRIE; Fiscal Year: 2010
    ..abstract_text> ..
  29. Discovery of Epigenetic Marks in Human Cells by High Throughput siRNA Screening
    RICHARD ALAN KATZ; Fiscal Year: 2010
    ..This process, termed "epigenetic silencing," is reversible, and the goal of the proposed research is to identify novel cellular processes that cause epigenetic silencing such that new therapies can be devised. ..
  30. Identification of Host Genes for Retroviral Replication
    Suzanne Sandmeyer; Fiscal Year: 2006
    ..This approach has high potential for opening unexpected avenues in retrovirus research and identifying novel targets for therapeutics. ..
  31. Ras signaling in leukemogenesis
    Ruibao Ren; Fiscal Year: 2010
    ..The ultimate goal of these studies is to identify critical molecular events in Ras leukemogenesis, allowing therapeutic interventions of leukemias involving Ras. ..
  32. mTOR Signaling In Chronic Myelogenous Leukemia
    Hung Fan; Fiscal Year: 2009
    ..abstract_text> ..
  33. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  34. Retrovirus Restriction by Trima5alpha
    Paul Bieniasz; Fiscal Year: 2009
    ....
  35. MOLECULAR MECHANISMS IN NEOPLASIA
    Paul Neiman; Fiscal Year: 2008
    ..In this fashion we hope to establish whether Myc-induced genomic instability merits wider exploration in human neoplasm. [unreadable] [unreadable] [unreadable] [unreadable]..
  36. LENTIVIRAL VARIATION AND AIDS
    Julie Overbaugh; Fiscal Year: 2008
    ..Collectively these studies will define the interplay between the properties of the infecting virus, the immune response and replication fitness in SIV/HIV pathogenesis. ..
  37. Role of cytokines in retroviral neuropathogenesis
    Karin Peterson; Fiscal Year: 2005
    ..Therefore, we will also analyze how infected microglia cells regulate TNF alpha and MCP-1production, in vivo. ..
  38. Peroxisome Membrane Biogenesis
    Stephen Gould; Fiscal Year: 2004
    ..abstract_text> ..
  39. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  40. Institute of Human Virology Annual Meeting 2002-2004
    Robert Gallo; Fiscal Year: 2002
    ..abstract_text> ..
  41. PREVENTION OF HIV SHEDDING IN WOMEN--TRIAL OF VITAMIN A
    Julie Overbaugh; Fiscal Year: 2003
    ..If this preliminary result is confirmed in a full-scale clinical trial, vitamin A supplementation may offer a simple inexpensive intervention to decrease heterosexual and vertical transmission of HIV-1 in the developing world. ..
  42. MOLECULAR BIOLOGY OF JAAGSIEKTE SHEEP RETROVIRUS
    Hung Fan; Fiscal Year: 2003
    ..These experiments will provide insight into JSRV-induced lung cancer in sheep, and possibly into human BAC. ..
  43. Workshop On Experimental Models from Bursa of Fabricius
    Paul Neiman; Fiscal Year: 2002
    ..This workshop will serve to stimulate further development and application, and to educate interested young scientists to the opportunities presented. ..
  44. INHIBITOR FORMATION IN GENE THERAPY FOR HEMOPHILIA
    Katherine High; Fiscal Year: 2001
    ..These studies will involve a collaboration between the P.I.'s lab and the laboratory of Dr. Hildegund Ertl, an immunologist with experience in characterizing immune responses in the setting of viral vectors. ..
  45. REGULATION OF FELV REPLICATION AND PATHOGENESIS
    Julie Overbaugh; Fiscal Year: 2004
    ..In this regard, it is striking that in both FeLV and HIV infection, there is selection for T-cell tropic, cytopathic variants in the host, despite the many differences between these viruses. ..
  46. EXPRESSION AND PATHOGENESIS OF MURINE LEUKEMIA VIRUS
    Hung Fan; Fiscal Year: 2005
    ..low expression. Together, these experiments will provide new and important insights into leukemogenesis by MuLVs. ..
  47. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
    ..abstract_text> ..
  48. REGULATION OF IMMUNOGLOBULIN GENE EXPRESSION IN B CELLS
    Ruibao Ren; Fiscal Year: 2003
    ..The rules of combinatorial transcription activation will also allow the design of novel transcription regulatory sequences to direct therapeutic expression of exogenous genes in selected cells. ..
  49. IDENTIFICATION OF TARGETS OF BCR ABL IN THE LEUKEMOGENIC
    Ruibao Ren; Fiscal Year: 2005
    ..These studies will help to further design rational therapeutic interventions for CML and to understand the mechanisms involved in leukemogenesis in general. ..
  50. Rapid screen for HIV TM membrane interactions/drugs
    Robert Garry; Fiscal Year: 2004
    ..The approaches piloted here may be useful for developing drugs against the AIDS virus and a broad range of important human viral pathogens with similar fusion proteins. ..
  51. HYPOXIC STRESS MECHANISMS IN RADIATION AND CHEMOTHERAPY
    Keith Laderoute; Fiscal Year: 2009
    ..An understanding of these MTF-1-dependent properties should aid in the development of novel prognostic and new modalities for neoplastic diseases. ..
  52. LENTIVIRAL VECTOR TRANSFER TO HEMATOPOIETIC STEM CELLS
    Donald Kohn; Fiscal Year: 2005
    ..This "intracellular immunization" approach may provide a therapeutic measure against HIV-1 that adds to pre-existing therapies, such as anti-retroviral drugs. ..
  53. ROLE OF AMPK IN TUMOR DEVELOPMENT AND THERAPY
    Keith R Laderoute; Fiscal Year: 2010
    ..Ultimately, we intend to determine whether the AMPK "emergency stress response" can be exploited for the therapy or prognosis of human cancer. ..
  54. CONFERENCE--AQUATIC ANIMAL MODELS OF HUMAN DISEASE
    MICHAEL SCHMALE; Fiscal Year: 2003
    ..The investigators strongly believe the forum provided by this type of interdisciplinary meeting is unique and is critical to the advancement of these diverse model systems as useful resources for the biomedical research community. ..
  55. Novel Approaches to Detect Virus-Cancer Associations
    Paul Ahlquist; Fiscal Year: 2005
    ..The methods developed also will be applicable to probe for virus involvement in other chronic or acute disorders. ..
  56. HIV-1 TROPISM AND CHEMOKINE RECEPTORS
    BRYAN CULLEN; Fiscal Year: 2002
    ..The final specific aim focusses on the promoter regions of CCR5, the identification of transcription factors that may interact with the promoter, and the use of antisense strategies to block CCR5 expression. ..
  57. Enhancement of the glycosylation machinery of the hen bioreactor.
    ALEX HARVEY; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  58. Enhancement of Gene Therapy Outcomes for Fabry Disease
    Jeffrey Medin; Fiscal Year: 2004
    ..Hypothesis: Co-overexpression of the prosaposin gene, a protein co-factor for the a-gal A enzyme, leads to higher catalytic activity in genetically corrected cells than overexpression of a-gal A alone. ..
  59. Cytocidal Therapy In Vivo for Drug-Resistant Tumors
    Jason Koutcher; Fiscal Year: 2007
    ..If validated clinically, the proposed therapy will open the way for cure of metastatic breast cancer, and the therapeutic strategy likely will apply to other drug-resistant types of cancer. ..
  60. Optimizing Chemotherapy Dose Using 31P NMR Spectroscopy
    Jason Koutcher; Fiscal Year: 2006
    ..If measurements of 6PG in peripheral blood lymphocytes correlate with tumor 6PG, future studies would not require further NMR studies. [unreadable] [unreadable]..
  61. MUTATIONS IN DEVELOPMENTAL PATHWAYS BY ENU MUTAGENESIS
    Monica Justice; Fiscal Year: 2005
    ..Further, models of pediatric metabolic disorders and endocrinopathies will be isolated and characterized. ..
  62. Lentivirus Gene Therapy for Farber Disease in NHPs
    Jeffrey Medin; Fiscal Year: 2007
    ..This proposed study would thus be the first to fully test LV-mediatedcorrection for an LSD in a more clinically relevant model; that of NHPs. ..
  63. FOAMY VIRUS INFECTION IN VITRO AND IN VIVO
    Maxine Linial; Fiscal Year: 2005
    ..These experiments will be will be important for the assessment of the utility of foamy viruses as vectors for gene therapy. ..
  64. TLS and TLS Fusion Proteins in Leukemia
    Liu Yang; Fiscal Year: 2005
    ..It is envisioned that successful completion of this project will unveil potential therapeutic targets in the treatment of human leukemias characterized by the t(16;21) translocation. ..
  65. Retrovirus Isolation from Primary Biliary Cirrhosis
    Andrew Mason; Fiscal Year: 2004
    ....
  66. The Enzymology of M-MuLV and HIV Replication
    JAMES CHAMPOUX; Fiscal Year: 2009
    ..The analysis of reverse transcriptase fidelity during displacement synthesis will directly contribute to our understanding of how the virus rapidly evolves during progression to AIDS. ..
  67. GENE THERAPY FOR HURLERS DISEASE
    Donald Kohn; Fiscal Year: 2001
    ..Successful tolerance induction in murine and canine models of MPS I will be an Important step toward application of gene therapy in human patients. ..
  68. EUKARYOTIC TOPOISOMERASE I
    James J Champoux; Fiscal Year: 2010
    ....
  69. DNA BINDING BY HUMAN TOPOISOMERASE I
    JAMES CHAMPOUX; Fiscal Year: 2003
    ..abstract_text> ..
  70. CHONDROGENESIS AND HISTONE MODIFICATION ENZYMES
    Liu Yang; Fiscal Year: 2007
    ....
  71. Protein Therapy for Junctional Epidermolysis Bullosa
    Vitali Alexeev; Fiscal Year: 2006
    ..abstract_text> ..
  72. Cellular Response to Retroviral DNA Integration
    Rene Daniel; Fiscal Year: 2007
    ..They should also significantly enhance our understanding of the function of these important cellular pathways. [unreadable] [unreadable]..
  73. Multifunctional nanoparticles for targeted DNA vaccine delivery
    Chun Wang; Fiscal Year: 2008
    ..With further development, the proposed nanoparticle technology is expected to have a significant impact on gene-based immunotherapy for treating cancer and infectious diseases. [unreadable] [unreadable] [unreadable]..
  74. Polymer-based Controlled Release Systems: Gene Delivery
    Chun Wang; Fiscal Year: 2004
    ..abstract_text> ..