sars virus

Summary

Summary: A species of CORONAVIRUS causing atypical respiratory disease (SEVERE ACUTE RESPIRATORY SYNDROME) in humans. The organism is believed to have first emerged in Guangdong Province, China, in 2002.

Top Publications

  1. ncbi Bats are natural reservoirs of SARS-like coronaviruses
    Wendong Li
    Institute of Zoology, Chinese Academy of Sciences CAS, Beijing, China
    Science 310:676-9. 2005
  2. pmc A transmembrane serine protease is linked to the severe acute respiratory syndrome coronavirus receptor and activates virus entry
    Ana Shulla
    Department of Microbiology and Immunology, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USA
    J Virol 85:873-82. 2011
  3. ncbi Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Christian Drosten
    Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases, Hamburg, Germany
    N Engl J Med 348:1967-76. 2003
  4. pmc In vitro reconstitution of SARS-coronavirus mRNA cap methylation
    Mickaël Bouvet
    Architecture et Fonction des Macromolecules Biologiques, CNRS and Universités d Aix Marseille I et II, UMR 6098, ESIL case 925, Marseille, France
    PLoS Pathog 6:e1000863. 2010
  5. pmc Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus
    Boyd Yount
    Department of Epidemiology and Microbiology, University of North Carolina, Chapel Hill, NC 27599 7435, USA
    Proc Natl Acad Sci U S A 100:12995-3000. 2003
  6. pmc Inhibitors of cathepsin L prevent severe acute respiratory syndrome coronavirus entry
    Graham Simmons
    Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 102:11876-81. 2005
  7. pmc Recombination, reservoirs, and the modular spike: mechanisms of coronavirus cross-species transmission
    Rachel L Graham
    Department of Epidemiology, School of Public Health, University of North Carolina at Chapel Hill, 2105D McGaveran Greenberg Hall, CB7, Chapel Hill, NC 27599, USA
    J Virol 84:3134-46. 2010
  8. pmc Severe acute respiratory syndrome coronavirus nsp1 suppresses host gene expression, including that of type I interferon, in infected cells
    Krishna Narayanan
    Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555 1019, USA
    J Virol 82:4471-9. 2008
  9. pmc Severe acute respiratory syndrome coronavirus triggers apoptosis via protein kinase R but is resistant to its antiviral activity
    Verena Krähling
    Department of Virology, Philipps University Marburg, Hans Meerwein Strasse 2, 35043 Marburg, Germany
    J Virol 83:2298-309. 2009
  10. pmc DC-SIGN and DC-SIGNR interact with the glycoprotein of Marburg virus and the S protein of severe acute respiratory syndrome coronavirus
    Andrea Marzi
    Institute for Clinical and Molecular Virology, University Erlangen Nurnberg, Nikolaus Fiebiger Center, Glückstrasse 6, D 91054 Erlangen, Germany
    J Virol 78:12090-5. 2004

Research Grants

  1. Viral and Host Determinants of New World Alphaviral Entry
    ASIM AMINSHARIF AHMED; Fiscal Year: 2013
  2. NOX1 and NOX2 as Therapeutic Targets in Influenza
    JOHN DAVID LAMBETH; Fiscal Year: 2013
  3. Coronavirus RNA Replication
    DAVID ALVIN BRIAN; Fiscal Year: 2012
  4. Molecular Biology of Eukaryotic Viruses
    Bert L Semler; Fiscal Year: 2012
  5. IMMUNITY AND VIRUS DISEASE
    Raymond M Welsh; Fiscal Year: 2013
  6. Immune Regulation of Virus Clearance and Tissue Injury at Sites of Infection
    Thomas J Braciale; Fiscal Year: 2013
  7. The Mechanisms of IFITM-Mediated Restriction
    I Chueh Huang; Fiscal Year: 2013
  8. Point of care microfluidic diagnosis system for rapid and sensitive detection of
    Michel G Bergeron; Fiscal Year: 2013
  9. Yeast Based Assays for Chemical Screens Against SARS-CoV Targets
    DANIEL A contact ENGEL; Fiscal Year: 2010
  10. MOLECULAR BIOLOGY OF CORONAVIRUS INDUCED DEMYELINATION
    Susan R Weiss; Fiscal Year: 2013

Detail Information

Publications359 found, 100 shown here

  1. ncbi Bats are natural reservoirs of SARS-like coronaviruses
    Wendong Li
    Institute of Zoology, Chinese Academy of Sciences CAS, Beijing, China
    Science 310:676-9. 2005
    ..The human and civet isolates of SARS-CoV nestle phylogenetically within the spectrum of SL-CoVs, indicating that the virus responsible for the SARS outbreak was a member of this coronavirus group...
  2. pmc A transmembrane serine protease is linked to the severe acute respiratory syndrome coronavirus receptor and activates virus entry
    Ana Shulla
    Department of Microbiology and Immunology, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USA
    J Virol 85:873-82. 2011
    ..These findings indicate that a cell surface complex comprising a primary receptor and a separate endoprotease operates as a portal for activation of SARS-CoV cell entry...
  3. ncbi Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Christian Drosten
    Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases, Hamburg, Germany
    N Engl J Med 348:1967-76. 2003
    ..The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent...
  4. pmc In vitro reconstitution of SARS-coronavirus mRNA cap methylation
    Mickaël Bouvet
    Architecture et Fonction des Macromolecules Biologiques, CNRS and Universités d Aix Marseille I et II, UMR 6098, ESIL case 925, Marseille, France
    PLoS Pathog 6:e1000863. 2010
    ....
  5. pmc Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus
    Boyd Yount
    Department of Epidemiology and Microbiology, University of North Carolina, Chapel Hill, NC 27599 7435, USA
    Proc Natl Acad Sci U S A 100:12995-3000. 2003
    ....
  6. pmc Inhibitors of cathepsin L prevent severe acute respiratory syndrome coronavirus entry
    Graham Simmons
    Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 102:11876-81. 2005
    ..The requirement for cathepsin L proteolysis identifies a previously uncharacterized class of inhibitor for SARS-CoV infection...
  7. pmc Recombination, reservoirs, and the modular spike: mechanisms of coronavirus cross-species transmission
    Rachel L Graham
    Department of Epidemiology, School of Public Health, University of North Carolina at Chapel Hill, 2105D McGaveran Greenberg Hall, CB7, Chapel Hill, NC 27599, USA
    J Virol 84:3134-46. 2010
    ..We pay particular attention to how changes in the Spike attachment protein, both within and outside of the receptor binding domain, mediate the emergence of coronaviruses in new host populations...
  8. pmc Severe acute respiratory syndrome coronavirus nsp1 suppresses host gene expression, including that of type I interferon, in infected cells
    Krishna Narayanan
    Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555 1019, USA
    J Virol 82:4471-9. 2008
    ..These data demonstrated that SARS-CoV nsp1 suppressed host innate immune functions, including type I IFN expression, in infected cells and suggested that SARS-CoV nsp1 most probably plays a critical role in SARS-CoV virulence...
  9. pmc Severe acute respiratory syndrome coronavirus triggers apoptosis via protein kinase R but is resistant to its antiviral activity
    Verena Krähling
    Department of Virology, Philipps University Marburg, Hans Meerwein Strasse 2, 35043 Marburg, Germany
    J Virol 83:2298-309. 2009
    ..Furthermore, our data suggest that viral activation of PKR can lead to apoptosis via a pathway that is independent of eIF2alpha phosphorylation...
  10. pmc DC-SIGN and DC-SIGNR interact with the glycoprotein of Marburg virus and the S protein of severe acute respiratory syndrome coronavirus
    Andrea Marzi
    Institute for Clinical and Molecular Virology, University Erlangen Nurnberg, Nikolaus Fiebiger Center, Glückstrasse 6, D 91054 Erlangen, Germany
    J Virol 78:12090-5. 2004
    ..SIGNR1, a murine DC-SIGN homologue, also enhanced infection driven by MARV and Ebola virus GP and could be targeted to assess the role of attachment factors in filovirus infection in vivo...
  11. ncbi A review of studies on animal reservoirs of the SARS coronavirus
    Zhengli Shi
    State Key Laboratory of Virology and Joint Lab of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China
    Virus Res 133:74-87. 2008
    ..Apart from masked palm civets and bats, 29 other animal species had been tested for the SARS-CoV, and the results are summarized in this paper...
  12. pmc Achieving a golden mean: mechanisms by which coronaviruses ensure synthesis of the correct stoichiometric ratios of viral proteins
    Ewan P Plant
    Laboratory of Hepatitis and Related Emerging Agents, Division of Emerging and Transfusion Transmitted Diseases, Office of Blood Research and Review, CBER, FDA, 8800 Rockville Pike, HFM310, Bethesda, Maryland 20892, USA
    J Virol 84:4330-40. 2010
    ..The findings of these analyses all support a "golden mean" model in which viruses use both programmed ribosomal frameshifting and translational attenuation to control the relative ratios of their encoded proteins...
  13. pmc Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells
    Amy C Sims
    Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    J Virol 87:3885-902. 2013
    ....
  14. ncbi Identification of a minimal peptide derived from heptad repeat (HR) 2 of spike protein of SARS-CoV and combination of HR1-derived peptides as fusion inhibitors
    I Jung Liu
    Institute of Microbiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    Antiviral Res 81:82-7. 2009
    ..39+/-0.05 microM and combination index of 0.75+/-0.15, suggesting a common strategy to achieve promising inhibition by HR1 peptide for other class I envelope viruses...
  15. pmc SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells
    Eric C Mossel
    Colorado State University, Fort Collins, CO 80523, USA
    Virology 372:127-35. 2008
    ..Hence, differentiated adult human alveolar type II cells were infectible but alveolar type I-like cells and alveolar macrophages did not support productive infection...
  16. pmc A double-inactivated severe acute respiratory syndrome coronavirus vaccine provides incomplete protection in mice and induces increased eosinophilic proinflammatory pulmonary response upon challenge
    Meagan Bolles
    Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    J Virol 85:12201-15. 2011
    ....
  17. ncbi Aetiology: Koch's postulates fulfilled for SARS virus
    Ron A M Fouchier
    Department of Virology, Erasmus Medical Centre, 3015 GE Rotterdam, The Netherlands
    Nature 423:240. 2003
  18. pmc The RNA polymerase activity of SARS-coronavirus nsp12 is primer dependent
    Aartjan J W te Velthuis
    Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Nucleic Acids Res 38:203-14. 2010
    ..The SARS-CoV nsp12 is primer dependent on both homo- and heteropolymeric templates, supporting the likeliness of a close enzymatic collaboration with the intriguing RNA primase activity that was recently proposed for coronavirus nsp8...
  19. ncbi Comparative evaluation of two severe acute respiratory syndrome (SARS) vaccine candidates in mice challenged with SARS coronavirus
    Raymond H See
    University of British Columbia Centre for Disease Control, Vancouver, BC V5Z 4R4, Canada
    J Gen Virol 87:641-50. 2006
    ..Finally, the sera of vaccinated mice contained antibodies to S, further suggesting a role for this protein in conferring protective immunity against SARS-CoV infection...
  20. pmc A noncovalent class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication
    Kiira Ratia
    Center for Pharmaceutical Biotechnology and Department of Medicinal Chemistry and Pharmacognosy, University of Illinois, Chicago, IL 60607, USA
    Proc Natl Acad Sci U S A 105:16119-24. 2008
    ....
  21. ncbi Lung pathology of fatal severe acute respiratory syndrome
    John M Nicholls
    Department of Pathology, University of Hong Kong, Hong Kong Special Administrative Region, China
    Lancet 361:1773-8. 2003
    ..Severe acute respiratory syndrome (SARS) is a novel infectious disease with global impact. A virus from the family Coronaviridae has been identified as the cause, but the pathogenesis is still unclear...
  22. ncbi Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus
    Wenhui Li
    Partners AIDS Research Center, Brigham and Women s Hospital, Department of Medicine Microbiology and Molecular Genetics, Boston, Massachusetts 02115, USA
    Nature 426:450-4. 2003
    ..Finally, anti-ACE2 but not anti-ACE1 antibody blocked viral replication on Vero E6 cells. Together our data indicate that ACE2 is a functional receptor for SARS-CoV...
  23. pmc Ecoepidemiology and complete genome comparison of different strains of severe acute respiratory syndrome-related Rhinolophus bat coronavirus in China reveal bats as a reservoir for acute, self-limiting infection that allows recombination events
    Susanna K P Lau
    State Key Laboratory of Emerging Infectious Diseases, Research Centre of Infection and Immunology, Department of Microbiology, The University of Hong Kong, Hong Kong, Hong Kong
    J Virol 84:2808-19. 2010
    ..Such frequent recombination, coupled with rapid evolution especially in ORF7b/ORF8 region, in these animals may have accounted for the cross-species transmission and emergence of SARS...
  24. pmc The spike protein of SARS-CoV--a target for vaccine and therapeutic development
    Lanying Du
    Lindsley F Kimball Research Institute, New York Blood Center, 310 East 67th Street, New York, NY 10065, USA
    Nat Rev Microbiol 7:226-36. 2009
    ..In this Review, we highlight recent advances in the development of vaccines and therapeutics based on the S protein...
  25. ncbi Immunopathogenesis of coronavirus infections: implications for SARS
    Stanley Perlman
    Interdisciplinary Program in Immunology, University of Iowa, Iowa City, Iowa 52242, USA
    Nat Rev Immunol 5:917-27. 2005
    ..It is hoped that lessons from such studies will help us to understand more about the pathogenesis of SARS in humans and to prevent or control outbreaks of SARS in the future...
  26. pmc Synthetic recombinant bat SARS-like coronavirus is infectious in cultured cells and in mice
    Michelle M Becker
    Departments of Pediatrics and Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 105:19944-9. 2008
    ....
  27. ncbi SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells
    I Chueh Huang
    Pulmonary Division, Children s Hospital, Children s Hospital Laboratory of Molecular Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:3198-203. 2006
    ..Our data indicate that two coronaviruses that utilize a common receptor nonetheless enter cells through distinct mechanisms...
  28. ncbi A novel coronavirus associated with severe acute respiratory syndrome
    Thomas G Ksiazek
    Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USA
    N Engl J Med 348:1953-66. 2003
    ..We conducted studies to identify the etiologic agent of this outbreak...
  29. pmc Severe acute respiratory syndrome coronavirus open reading frame (ORF) 3b, ORF 6, and nucleocapsid proteins function as interferon antagonists
    Sarah A Kopecky-Bromberg
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    J Virol 81:548-57. 2007
    ..ORF 6 protein, but not ORF 3b or N protein, inhibited nuclear translocation but not phosphorylation of STAT1. Thus, it appears that these three interferon antagonists of SARS-CoV inhibit the interferon response by different mechanisms...
  30. ncbi Binding mechanism of coronavirus main proteinase with ligands and its implication to drug design against SARS
    Kuo Chen Chou
    Gordon Life Science Institute, 7088 Arbor Valley, Kalamazoo, MI 49009, USA
    Biochem Biophys Res Commun 308:148-51. 2003
    ..Meanwhile, the idea of how to develop inhibitors of the SARS enzyme based on the knowledge of its own peptide substrates (the so-called "distorted key" approach) was also briefly elucidated...
  31. pmc Severe acute respiratory syndrome coronavirus evades antiviral signaling: role of nsp1 and rational design of an attenuated strain
    Marc G Wathelet
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0576, USA
    J Virol 81:11620-33. 2007
    ..However, mutant virus replication was strongly attenuated in cells with an intact IFN response. Thus, nsp1 is likely a virulence factor that contributes to pathogenicity by favoring SARS-CoV replication...
  32. pmc The papain-like protease of severe acute respiratory syndrome coronavirus has deubiquitinating activity
    Naina Barretto
    Department of Microbiology and Immunology, Loyola University Chicago, Stritch School of Medicine, 2160 South First Avenue, Bldg 105, Maywood, IL 60153, USA
    J Virol 79:15189-98. 2005
    ..This similarity in the substrate recognition sites should be considered during the development of SARS-CoV PLpro inhibitors...
  33. pmc Entry from the cell surface of severe acute respiratory syndrome coronavirus with cleaved S protein as revealed by pseudotype virus bearing cleaved S protein
    Rie Watanabe
    Department of Virology III, National Institute of Infectious Diseases, Murayama Branch, 4 7 1 Gakuen, Mushai Murayama, Tokyo 208 0011, Japan
    J Virol 82:11985-91. 2008
    ..Those results indicate that SARS-CoV with a cleaved S protein is able to enter cells directly from the cell surface and agree with the previous observation of the protease-mediated cell surface entry of SARS-CoV...
  34. pmc Inhibition of Beta interferon induction by severe acute respiratory syndrome coronavirus suggests a two-step model for activation of interferon regulatory factor 3
    Martin Spiegel
    Abteilung Virologie, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Freiburg, D 79008 Freiburg, Germany
    J Virol 79:2079-86. 2005
    ..In order to escape activation of the IFN system, SARS-CoV appears to block a step after the early nuclear transport of IRF-3...
  35. pmc Distinct severe acute respiratory syndrome coronavirus-induced acute lung injury pathways in two different nonhuman primate species
    Saskia L Smits
    Department of Virology, Erasmus Medical Center, P O Box 2040, 3000 CA Rotterdam, Netherlands
    J Virol 85:4234-45. 2011
    ..Induction of distinct proinflammatory genes after SARS-CoV infection in different nonhuman primate species needs to be taken into account when analyzing outcomes of intervention strategies in these species...
  36. pmc Supramolecular architecture of severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy
    Benjamin W Neuman
    Department of Molecular and Integrative Neuroscience, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
    J Virol 80:7918-28. 2006
    ..Our results contribute to the understanding of the assembly pathway used by coronaviruses and other pleomorphic viruses and provide the first detailed view of coronavirus ultrastructure...
  37. pmc Mice susceptible to SARS coronavirus
    David E Wentworth
    New York Department of Health, Albany 12201 2002, USA
    Emerg Infect Dis 10:1293-6. 2004
    ..When BALB/c mice were simultaneously inoculated intranasally and orally, replication of SARS-CoV was found in both lung and intestinal tissue...
  38. pmc A 219-mer CHO-expressing receptor-binding domain of SARS-CoV S protein induces potent immune responses and protective immunity
    Lanying Du
    Lindsley F Kimball Research Institute, New York Blood Center, New York, New York 10065, USA
    Viral Immunol 23:211-9. 2010
    ..These results suggest that the recombinant RBD219-CHO protein has great potential for the development of an effective and safe SARS subunit vaccine...
  39. pmc Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodies
    Hong Zhang
    Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, China
    J Virol 78:6938-45. 2004
    ..Identification of a conserved antigenic determinant on the S2 domain of the SARS-CoV S protein, which has the potential for inducing neutralizing antibodies, has implications in the development of effective vaccines against SARS-CoV...
  40. pmc Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice
    Baojing Lu
    State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan
    Immunology 130:254-61. 2010
    ....
  41. pmc Self-assembly of severe acute respiratory syndrome coronavirus membrane protein
    Ying Tzu Tseng
    Department of Medical Research and Education, Taipei Veterans General Hospital, Taiwan
    J Biol Chem 285:12862-72. 2010
    ..The data suggest that multiple SARS-CoV M regions are involved in M self-assembly and subcellular localization...
  42. ncbi Prior immunization with severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV
    Fumihiko Yasui
    Department of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Japan
    J Immunol 181:6337-48. 2008
    ..These findings increase our understanding of the pathogenesis of SARS...
  43. pmc The SARS-coronavirus nsp7+nsp8 complex is a unique multimeric RNA polymerase capable of both de novo initiation and primer extension
    Aartjan J W te Velthuis
    Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, PO Box 9600, 2300RC Leiden, The Netherlands
    Nucleic Acids Res 40:1737-47. 2012
    ..Finally, site-directed mutagenesis of conserved D/ExD/E motifs was employed to identify residues crucial for nsp(7+8) RdRp activity...
  44. pmc Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis
    Chung Y Cheung
    Department of Microbiology, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong, Special Administrative Region, People s Republic of China
    J Virol 79:7819-26. 2005
    ..The poor induction of IFN-beta, a key component of innate immunity, and the ability of the virus to induce chemokines could explain aspects of the pathogenesis of SARS...
  45. ncbi SARS corona virus peptides recognized by antibodies in the sera of convalescent cases
    Jian Ping Guo
    Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Virology 324:251-6. 2004
    ..The findings have implications for monitoring humoral responses to SARS-CoV as well as for developing a successful SARS vaccine...
  46. pmc Immunization with SARS coronavirus vaccines leads to pulmonary immunopathology on challenge with the SARS virus
    CHIEN TE TSENG
    Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America
    PLoS ONE 7:e35421. 2012
    ..Evaluations of an inactivated whole virus vaccine in ferrets and nonhuman primates and a virus-like-particle vaccine in mice induced protection against infection but challenged animals exhibited an immunopathologic-type lung disease...
  47. ncbi Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus
    Seisuke Hatakeyama
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Tokyo, Japan
    Virology 380:99-108. 2008
    ..These results indicated that the minimum portion of N required for the interaction with M and pseudoparticle formation consists of amino acids 301-422...
  48. pmc Severe acute respiratory syndrome coronavirus ORF6 antagonizes STAT1 function by sequestering nuclear import factors on the rough endoplasmic reticulum/Golgi membrane
    Matthew Frieman
    Department of Epidemiology, School of Public Health, University of North Carolina at Chapel Hill, 3304 Hooker Research Center, Chapel Hill, NC 27599 7435, USA
    J Virol 81:9812-24. 2007
    ..We discuss the likely implications of these data on SARS-CoV replication and pathogenesis...
  49. ncbi Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein
    José L Nieto-Torres
    Department of Molecular and Cell Biology, Centro Nacional de Biotecnologia CNB CSIC, Darwin 3, Campus Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Virology 415:69-82. 2011
    ..A topological conformation in which SARS-CoV E protein amino terminus is oriented towards the lumen of intracellular membranes and carboxy terminus faces cell cytoplasm is proposed...
  50. pmc Severe acute respiratory syndrome coronavirus M protein inhibits type I interferon production by impeding the formation of TRAF3.TANK.TBK1/IKKepsilon complex
    Kam Leung Siu
    Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong
    J Biol Chem 284:16202-9. 2009
    ..Taken together, our findings reveal a new mechanism by which SARS coronavirus circumvents the production of type I interferons...
  51. pmc Chimeric severe acute respiratory syndrome coronavirus (SARS-CoV) S glycoprotein and influenza matrix 1 efficiently form virus-like particles (VLPs) that protect mice against challenge with SARS-CoV
    Ye V Liu
    Novavax Inc, 9920 Belward Campus Drive, Rockville, MD 20850, United States
    Vaccine 29:6606-13. 2011
    ..We developed a novel method to produce high levels of a recombinant SARS virus-like particles (VLPs) vaccine containing the SARS spike (S) protein and the influenza M1 protein using the ..
  52. ncbi A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses
    Martin Spruth
    Baxter Vaccines, Biomedical Research Centre, Uferstr 15, 2304 Orth Donau, Austria
    Vaccine 24:652-61. 2006
    ..Protection of mice was correlated to antibody titre against the SARS-CoV S protein and neutralising antibody titre...
  53. ncbi Severe acute respiratory syndrome coronavirus nucleocapsid protein confers ability to efficiently produce virus-like particles when substituted for the human immunodeficiency virus nucleocapsid domain
    Shui Mei Wang
    Institute of Public Health, National Yang Ming University, Taipei, Taiwan, ROC
    J Biomed Sci 15:719-29. 2008
    ..The results suggest that the domain conferring the ability to direct VLP assembly and release in SARS-CoV N is largely contained between residues 168 and 421...
  54. ncbi Genetic analysis of the SARS-coronavirus spike glycoprotein functional domains involved in cell-surface expression and cell-to-cell fusion
    Chad M Petit
    Division of Biotechnology and Molecular Medicine BIOMMED, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA
    Virology 341:215-30. 2005
    ..This genetic analysis reveals that the SARS-CoV S glycoprotein contains extracellular domains that regulate cell fusion as well as distinct endodomains that function in intracellular transport, cell-surface expression, and cell fusion...
  55. pmc Molecular determinants for subcellular localization of the severe acute respiratory syndrome coronavirus open reading frame 3b protein
    Eric C Freundt
    Laboratory of Immunology, Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 83:6631-40. 2009
    ..The findings reported here reveal that for multilocalized proteins, consideration of the spatiotemporal distribution may be crucial for understanding viral protein behavior and function...
  56. pmc Identification of Hepta- and Octo-Uridine stretches as sole signals for programmed +1 and -1 ribosomal frameshifting during translation of SARS-CoV ORF 3a variants
    Xiaoxing Wang
    Department of Biological Sciences, National University of Singapore, Singapore 117543
    Nucleic Acids Res 34:1250-60. 2006
    ..Taken together, this study identifies the hepta- and octo-uridine stretches that function as sole elements for efficient +1 and -1 ribosomal frameshift events...
  57. ncbi Mitochondrial location of severe acute respiratory syndrome coronavirus 3b protein
    Xiaoling Yuan
    Department of Pathophysiology, Beijing Institute of Radiation Medicine, Beijing 100850, China
    Mol Cells 21:186-91. 2006
    ..These results provide new directions for studies of the role of SARS-CoV 3b protein in SARS pathogenesis...
  58. ncbi Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein forms a dimer through its C-terminal domain
    I Mei Yu
    Department of Biological Sciences and the Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 280:23280-6. 2005
    ..These results suggest that the N protein oligomerization involves the C-terminal residues 285-422, and this region is a good target for mutagenic studies to disrupt N protein self-association and virion assembly...
  59. pmc Cellular immune responses to severe acute respiratory syndrome coronavirus (SARS-CoV) infection in senescent BALB/c mice: CD4+ T cells are important in control of SARS-CoV infection
    Jun Chen
    Laboratory of Infectious Diseases, National Institute for Allergy and Infectious Diseases, NIH, Bethesda, Maryland 20892 3203, USA
    J Virol 84:1289-301. 2010
    ..Our findings provide new insights into the pathogenesis of SARS, demonstrating the important role of CD4(+) but not CD8(+) T cells in primary SARS-CoV infection in this model...
  60. ncbi Over-expression of severe acute respiratory syndrome coronavirus 3b protein induces both apoptosis and necrosis in Vero E6 cells
    Sehaam Khan
    Collaborative Anti Viral Research Group, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore
    Virus Res 122:20-7. 2006
    ..To our knowledge, this is the first report of the induction of necrosis by a SARS-CoV protein...
  61. ncbi Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development
    Hongyuan Shen
    Institute of Molecular and Cell Biology, Proteos, Singapore, Singapore
    J Virol Methods 160:48-56. 2009
    ..This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken...
  62. pmc Structural characterization of the fusion-active complex of severe acute respiratory syndrome (SARS) coronavirus
    Paolo Ingallinella
    Istituto di Ricerche di Biologia Molecolare P Angeletti, Via Pontina Km 30 600, 00040 Pomezia, Italy
    Proc Natl Acad Sci U S A 101:8709-14. 2004
    ..Inhibitors binding to HR regions of fusion proteins have been shown to be efficacious against many viruses, notably HIV. Our results may help in the design of anti-SARS therapeutics...
  63. ncbi Severe acute respiratory syndrome coronavirus papain-like protease suppressed alpha interferon-induced responses through downregulation of extracellular signal-regulated kinase 1-mediated signalling pathways
    Shih Wein Li
    Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan, ROC
    J Gen Virol 92:1127-40. 2011
    ..Overall results proved downregulation of ERK1 by ubiquitin proteasomes and suppression of interaction between ERK1 and STAT1 as type I IFN antagonist function of SARS-CoV PLpro...
  64. pmc The SARS coronavirus E protein interacts with PALS1 and alters tight junction formation and epithelial morphogenesis
    Kim Tat Teoh
    HKU Pasteur Research Centre, Pokfulam, Hong Kong SAR China
    Mol Biol Cell 21:3838-52. 2010
    ..We speculate that hijacking of PALS1 by SARS-CoV E plays a determinant role in the disruption of the lung epithelium in SARS patients...
  65. pmc Different host cell proteases activate the SARS-coronavirus spike-protein for cell-cell and virus-cell fusion
    Graham Simmons
    Blood Systems Research Institute and Department of Laboratory Medicine, University of California, San Francisco, CA, USA
    Virology 413:265-74. 2011
    ..Thus, different host cell proteases activate SARS-S for virus-cell and cell-cell fusion and SARS-S cleavage at R667 and 758-RXXR-762 can be dispensable for SARS-S activation...
  66. pmc Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus
    W C Yam
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Pokfulam, Hong Kong SAR, People s Republic of China
    J Clin Microbiol 41:4521-4. 2003
    ..Testing more than one respiratory specimen will maximize the sensitivity of PCR assays for SARS CoV...
  67. ncbi SARS-CoV accessory protein 3b induces AP-1 transcriptional activity through activation of JNK and ERK pathways
    Bhavna Varshney
    Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110018, India
    Biochemistry 50:5419-25. 2011
    ....
  68. ncbi SARS-CoV nucleocapsid protein antagonizes IFN-β response by targeting initial step of IFN-β induction pathway, and its C-terminal region is critical for the antagonism
    Xiaolu Lu
    State Key Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, Luojia Hill, Wuhan, People s Republic of China
    Virus Genes 42:37-45. 2011
    ..These results contribute to our further understanding of the pathogenesis of SARS-CoV...
  69. pmc Anti-severe acute respiratory syndrome coronavirus spike antibodies trigger infection of human immune cells via a pH- and cysteine protease-independent FcγR pathway
    Martial Jaume
    HKU Pasteur Research Centre, Dexter H C Man Building, 8 Sassoon Road, Pokfulam, Hong Kong SAR, China
    J Virol 85:10582-97. 2011
    ..Our results suggest a novel mechanism by which SARS-CoV can enter target cells and illustrate the potential pitfalls associated with immunization against it. These findings should prompt further investigations into SARS pathogenesis...
  70. ncbi Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus
    Xiaoling Yuan
    Department of Pathophysiology, Beijing Institute of Radiation Medicine, No 27 Taiping road, Beijing 100850, China
    Virus Res 114:70-9. 2005
    ..These results provide a new insight for further functional studies of SARS-CoV 3b protein...
  71. pmc Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses
    Yukinobu Tohya
    Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555 1019, USA
    J Virol 83:5282-8. 2009
    ..The results of our studies suggested a conserved function among nsp1 proteins of SARS-CoV and group 2 bat CoVs...
  72. ncbi SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway
    Hongliang Wang
    National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences, Beijing 100005, China
    Cell Res 18:290-301. 2008
    ..Endocytic entry of SARS-CoV may expand the cellular range of SARS-CoV infection, and our findings here contribute to the understanding of SARS-CoV pathogenesis, providing new information for anti-viral drug research...
  73. pmc The coronavirus spike protein induces endoplasmic reticulum stress and upregulation of intracellular chemokine mRNA concentrations
    Gijs A Versteeg
    Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, LUMC E4 P, P O Box 9600, 2300 RC Leiden, The Netherlands
    J Virol 81:10981-90. 2007
    ..However, at that point in infection, translation of host mRNA is already severely reduced in infected cells, preventing the synthesis of CXCL2 and ER stress proteins despite their increased mRNA concentrations...
  74. pmc Genomic characterization of severe acute respiratory syndrome-related coronavirus in European bats and classification of coronaviruses based on partial RNA-dependent RNA polymerase gene sequences
    Jan Felix Drexler
    Institute of Virology, University of Bonn Medical Centre, 53127 Bonn, Germany
    J Virol 84:11336-49. 2010
    ..Critical spike domains 472 and 487 were identical and similar, respectively. This study underlines the importance of assessments of the zoonotic potential of widely distributed bat-borne CoV...
  75. pmc Quantitative proteomics analysis reveals BAG3 as a potential target to suppress severe acute respiratory syndrome coronavirus replication
    Liang Zhang
    Division of Research, Singapore Health Research Facilities, 7 Hospital Drive, Singapore 169611, Republic of Singapore
    J Virol 84:6050-9. 2010
    ..By correlating the proteomic data with these functional studies, the findings of this study provide important information for understanding SARS-CoV replication...
  76. pmc Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV replication
    Ji An Pan
    State Key Laboratory of Virology and Modern Virology Research Centre, College of Life Sciences, Wuhan University, Wuhan, People s Republic of China
    PLoS ONE 3:e3299. 2008
    ..Collectively, our findings provide a basis and platform for further characterization of the functions and mechanisms of coronavirus proteins...
  77. ncbi The 3a accessory protein of SARS coronavirus specifically interacts with the 5'UTR of its genomic RNA, Using a unique 75 amino acid interaction domain
    Kulbhushan Sharma
    Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Road, New Delhi 110067, India
    Biochemistry 46:6488-99. 2007
    ..have shown that the 3a protein is capable of binding specifically to the 5' untranslated region (5'UTR) of the SARS virus genomic RNA...
  78. pmc Differential downregulation of ACE2 by the spike proteins of severe acute respiratory syndrome coronavirus and human coronavirus NL63
    Ilona Glowacka
    Institute of Virology, OE 5230, Hannover Medical School, Carl Neuberg Str 1, 30625 Hannover, Germany
    J Virol 84:1198-205. 2010
    ..Finally, SARS-CoV but not NL63 replicated efficiently in ACE2-positive Vero cells and reduced ACE2 expression, indicating robust receptor interference in the context of SARS-CoV but not NL63 infection...
  79. pmc Exacerbated innate host response to SARS-CoV in aged non-human primates
    Saskia L Smits
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    PLoS Pathog 6:e1000756. 2010
    ..Thus, ALI in SARS-CoV-infected aged macaques developed as a result of an exacerbated innate host response. The anti-inflammatory action of type I IFN reveals a potential intervention strategy for virus-induced ALI...
  80. pmc Molecular mapping of the RNA Cap 2'-O-methyltransferase activation interface between severe acute respiratory syndrome coronavirus nsp10 and nsp16
    Adrien Lugari
    IMR Laboratory, UPR 3243, Institut de Microbiologie de la Méditérannée, CNRS and Aix Marseille Universités, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Biol Chem 285:33230-41. 2010
    ..Thus, the nsp10-nsp16 interface may represent an attractive target for antivirals against human and animal pathogenic coronaviruses...
  81. pmc Viral evolution and the emergence of SARS coronavirus
    Edward C Holmes
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
    Philos Trans R Soc Lond B Biol Sci 359:1059-65. 2004
    ....
  82. pmc The nsp2 replicase proteins of murine hepatitis virus and severe acute respiratory syndrome coronavirus are dispensable for viral replication
    Rachel L Graham
    Department of Pediatrics, Vanderbilt University Medical Center, D6217 MCN, Nashville, TN 37232 2581, USA
    J Virol 79:13399-411. 2005
    ..These findings also provide a system for the study of determinants of nsp targeting and function...
  83. pmc SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum
    Kevin Knoops
    Section Electron Microscopy, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
    PLoS Biol 6:e226. 2008
    ..Together with biochemical studies of the viral enzyme complex, our ultrastructural description of this "replication network" will aid to further dissect the early stages of the coronavirus life cycle and its virus-host interactions...
  84. pmc Modulation of the unfolded protein response by the severe acute respiratory syndrome coronavirus spike protein
    Ching Ping Chan
    Department of Biochemistry, The University of Hong Kong, 3 F Laboratory Block, Faculty of Medicine Building, 21 Sassoon Road, Hong Kong
    J Virol 80:9279-87. 2006
    ..Taken together, our findings suggest that SARS-CoV S protein specifically modulates the UPR to facilitate viral replication...
  85. ncbi Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the severe-acute respiratory syndrome-coronavirus (SARS-CoV) receptor, angiotensin-converting enzyme-2 (ACE2)
    Daniel W Lambert
    Proteolysis Research Group, School of Biochemistry and Microbiology, University of Leeds, Leeds LS2 9JT, United Kingdom
    J Biol Chem 280:30113-9. 2005
    ..The identification of ADAM17 as the protease responsible for ACE2 shedding may provide new insight into the physiological roles of ACE2...
  86. ncbi The Immunobiology of SARS*
    Jun Chen
    Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, Maryland 20892, USA
    Annu Rev Immunol 25:443-72. 2007
    ..Innate immunity is important for viral clearance in the mouse model. Virus-specific neutralizing antibodies that develop during convalescence prevent reinfection in animal models...
  87. pmc Pathogenicity of severe acute respiratory coronavirus deletion mutants in hACE-2 transgenic mice
    Marta L DeDiego
    Department of Molecular and Cell Biology, Centro Nacional de Biotecnologia CSIC, Campus Universidad Autonoma, Darwin 3, Cantoblanco, 28049 Madrid, Spain
    Virology 376:379-89. 2008
    ..These data indicate that E gene might be a virulence factor influencing replication level, tissue tropism and pathogenicity of SARS-CoV, suggesting that DeltaE attenuated viruses are promising vaccine candidates...
  88. ncbi The SARS-Coronavirus PLnc domain of nsp3 as a replication/transcription scaffolding protein
    Isabelle Imbert
    Centre National de la Recherche Scientifique and Universités d Aix Marseille I et II, UMR 6098, Architecture et Fonction des Macromolecules Biologiques, Ecole Supérieure d Ingénieurs de Luminy Case 925, Marseille Cedex 9, France
    Virus Res 133:136-48. 2008
    ..The interaction map thus provides a sum of the interactions that may occur at some point during coronavirus RNA synthesis and provides a framework for future research...
  89. pmc Detection of novel SARS-like and other coronaviruses in bats from Kenya
    Suxiang Tong
    Gastroenteritis and Respiratory Virus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Emerg Infect Dis 15:482-5. 2009
    ..The sequence diversity suggests that bats are well-established reservoirs for and likely sources of coronaviruses for many species, including humans...
  90. pmc A severe acute respiratory syndrome coronavirus that lacks the E gene is attenuated in vitro and in vivo
    Marta L DeDiego
    Department of Molecular and Cell Biology, Centro Nacional de Biotecnologia, CSIC, Darwin 3, Campus Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
    J Virol 81:1701-13. 2007
    ..Therefore, the SARS-CoV that lacks the E gene is attenuated in hamsters, might be a safer research tool, and may be a good candidate for the development of a live attenuated SARS-CoV vaccine...
  91. pmc Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo
    Susanne Pfefferle
    Clinical Virology Group, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Virol J 6:131. 2009
    ..Because attenuation was focused on the early phase of infection in-vivo, ORF 7b might have contributed to the delayed accumulation of virus in patients that was suggested to have limited the spread of the SARS epidemic...
  92. pmc A severe acute respiratory syndrome-associated coronavirus-specific protein enhances virulence of an attenuated murine coronavirus
    Lecia Pewe
    Department of Pediatrics, University of Iowa, Medical Laboratories 2042, Iowa City, 52242, USA
    J Virol 79:11335-42. 2005
    ....
  93. ncbi Mechanism of the maturation process of SARS-CoV 3CL protease
    Min Feng Hsu
    Institute of Biochemical Sciences, National Taiwan University, Taipei 106
    J Biol Chem 280:31257-66. 2005
    ..Taken together, this study provides insights into the maturation process of the SARS 3CL(pro) from the polyprotein and design of new structure-based inhibitors...
  94. ncbi Pathogenetic mechanisms of severe acute respiratory syndrome
    Yong Guo
    Department of Pathology, School of Basic Medical Sciences, Peking Beijing University, 38 Xueyuan Road, 100083 Beijing, China
    Virus Res 133:4-12. 2008
    ..The implications of the proposed pathogenesis for prevention, diagnosis and therapy of the disease are discussed...
  95. pmc Conformational reorganization of the SARS coronavirus spike following receptor binding: implications for membrane fusion
    Daniel R Beniac
    Viral Diseases Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
    PLoS ONE 2:e1082. 2007
    ..The SARS-CoV spike provides an ideal model system to study receptor binding and membrane fusion in the native state, employing cryo-electron microscopy and single-particle image analysis...
  96. ncbi The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: expression and functional characterization
    Hua Geng
    Department of Biochemistry, MMW509C, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China
    FEBS Lett 579:6763-8. 2005
    ..Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis...
  97. ncbi Severe acute respiratory syndrome: identification of the etiological agent
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
    Trends Mol Med 9:325-7. 2003
  98. pmc Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2'-O-methyltransferase nsp10/nsp16 complex
    Etienne Decroly
    Centre National de la Recherche Scientifique and Université de la Méditerranée, UMR 6098, Architecture et Fonction des Macromolecules Biologiques, Marseille, France
    PLoS Pathog 7:e1002059. 2011
    ....
  99. ncbi Lack of support for an association between CLEC4M homozygosity and protection against SARS coronavirus infection
    Nelson Leung Sang Tang
    Nat Genet 39:691-2; author reply 694-6. 2007
  100. pmc Mild severe acute respiratory syndrome
    Gang Li
    Emerg Infect Dis 9:1182-3. 2003
  101. pmc Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus
    Jincun Zhao
    Department of Immunology, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100083, China
    J Virol 81:6079-88. 2007
    ..These data provide useful insights regarding immunity against SARS-CoV and have the potential to help guide the design of peptide-based vaccines...

Research Grants64

  1. Viral and Host Determinants of New World Alphaviral Entry
    ASIM AMINSHARIF AHMED; Fiscal Year: 2013
    ..Children's Hospital Boston is committed to this career development plan and has assured the candidate will be able to devote at least 85% full-time effort to the activities described in this proposal. ..
  2. NOX1 and NOX2 as Therapeutic Targets in Influenza
    JOHN DAVID LAMBETH; Fiscal Year: 2013
    ....
  3. Coronavirus RNA Replication
    DAVID ALVIN BRIAN; Fiscal Year: 2012
    ..Knowing in detail what molecular interactions lead to coronavirus replication and recombination, the focus of this proposal, are essential for the development of stable and safe recombinant vaccines and the design of anti-viral drugs. ..
  4. Molecular Biology of Eukaryotic Viruses
    Bert L Semler; Fiscal Year: 2012
    ....
  5. IMMUNITY AND VIRUS DISEASE
    Raymond M Welsh; Fiscal Year: 2013
    ..abstract_text> ..
  6. Immune Regulation of Virus Clearance and Tissue Injury at Sites of Infection
    Thomas J Braciale; Fiscal Year: 2013
    ..To determine the impact of viral infection on the production of Te-derived IL- 10. The proposed studies are designed to complement ongoing related studies in Projects 2, 3 and 4. ..
  7. The Mechanisms of IFITM-Mediated Restriction
    I Chueh Huang; Fiscal Year: 2013
    ..I am situated in a highly supportive and intellectually rich environment in which I can pursue my scientific and career development goals. ..
  8. Point of care microfluidic diagnosis system for rapid and sensitive detection of
    Michel G Bergeron; Fiscal Year: 2013
    ..These POCTs will help public health authorities to define new operational strategies for both lab-based and field-based applications. ..
  9. Yeast Based Assays for Chemical Screens Against SARS-CoV Targets
    DANIEL A contact ENGEL; Fiscal Year: 2010
    ..We hypothesize that we can learn about the biology of SARS-CoV by identifying which chemical compounds can inhibit it, and then study how they work. ..
  10. MOLECULAR BIOLOGY OF CORONAVIRUS INDUCED DEMYELINATION
    Susan R Weiss; Fiscal Year: 2013
    ..Such studies will be applicable to understanding the pathogenesis of virus-induced CNS disease and, in the long term, contribute to vaccine design. ..
  11. Role of anti-SARS-CoV T cell response in pathogenesis
    Stanley Perlman; Fiscal Year: 2013
    ..Identification of rDC defects as critical in the increased susceptibility to viral respiratory infections will provide a novel therapeutic target in infected patients. ..
  12. STRUCTURE AND ASSEMBLY OF VIRUSES
    STEPHEN COPLAN HARRISON; Fiscal Year: 2012
    ..abstract_text> ..
  13. SARS Coronavirus: Inhibition of Entry
    Kathryn Holmes; Fiscal Year: 2009
    ..This research will help to explain the origin of SARS-CoV, explain the pathology of SARS, develop candidate vaccines and drugs against SARS, and test them in new animal models for SARS. ..
  14. Inhibition of the Innate Immune Response by the SARS Coronavirus
    MATTHEW BRYAN FRIEMAN; Fiscal Year: 2010
    ..Using a newly created mouse adapted SARS virus, which unlike the wild type isolates of SARS, does cause significant pathology and death in mice, we will ..
  15. Correlation of Sendai Virus Mutations with Pathogenicity
    NANCY LUGENE MCQUEEN; Fiscal Year: 2012
    With the emergence of new human viral pathogens such as the SARS virus, Nipah virus, and the avian influenza viruses, the importance of investigations on the genetic basis of viral infections becomes clear...
  16. Targeting matrix metalloproteinases to limit immunopathology in airborne infectio
    SUWAN JAYASINGHE; Fiscal Year: 2013
    ..g., SARS coronavirus). We will establish a new therapeutic paradigm targeting excessive host MMP activity to improve outcomes in pulmonary infection. ..
  17. Priming and fusion activation of the SARS coronavirus spike glycoprotein
    Gary R Whittaker; Fiscal Year: 2010
    ....
  18. Memory T cell subsets and pulmonary immunity
    David L Woodland; Fiscal Year: 2010
    ..for the generation of vaccines designed to promote protective immunity against pulmonary pathogens, such as SARS virus and avian influenza...
  19. Receptor recognition mechanisms of coronaviruses
    Fang Li; Fiscal Year: 2013
    ....
  20. Role of the Epithelial Growth Factor Receptor in SARS Coronavirus Pathogenesis
    MATTHEW BRYAN FRIEMAN; Fiscal Year: 2013
    ..These studies will identify the specific cell types that lead to acute lung disease induction and progression, allowing for cell directed therapies and targets interventions. ..
  21. Epidemiology of Infectious Diseases and Biodefense
    George R Seage; Fiscal Year: 2013
    ....
  22. Positive Strand RNA Viruses
    David L Woodland; Fiscal Year: 2013
    ....
  23. Determinants of AAV Lung Tropism
    Aravind Asokan; Fiscal Year: 2013
    ..If successful, this knowledge may facilitate improvements in AAV vectors as well as in the design of preclinical/clinical studies focused on gene therapy of airway diseases such as cystic fibrosis. ..
  24. SARS Coronavirus Virulence Factors and Lung Pathogenesis
    Marc G Wathelet; Fiscal Year: 2010
    ..abstract_text> ..
  25. Antigenic Relationships of SARS and Animal Coronaviruses
    Linda Saif; Fiscal Year: 2006
    ..from civet cats and raccoon dogs in China and from a recent human SARS case in China suggesting the latter SARS virus reemerged from an animal reservoir. SARS CoV experimentally infects macaques, ferrets and cats...
  26. Structure and Mechanism of Programmed Ribosomal Frameshifting in SARS coronavirus
    VICTORIA MANUEL D'SOUZA; Fiscal Year: 2013
    ....
  27. Task 19/D21 NHP FOR SARS CORONAVIRUS VACCINE AND DRUG TESTING
    KATHLEEN KOOTZ; Fiscal Year: 2009
    ..In addition, it can perform testing of therapeutics for safety and pharmacology, in small animals as well as non-human primates, under GLP where appropriate. ..
  28. EFFECTORS AND IINHIBITORS OF SARS VIRUS POLYMERASE
    Neerja Kaushik Basu; Fiscal Year: 2006
    ..Further, analysis of the sensitivity of the SARS-CoV RdRp towards a panel of known and novel inhibitors of polymerases will provide new leads in the quest for specific antiviral agents against the SARS-CoV RdRp. ..
  29. T Cell Function in Pulmonary Viral Pulmonary Infection and Injury
    Thomas J Braciale; Fiscal Year: 2012
    ..To define the mechanism through which Te-derived IL-10 regulates pulmonary inflammation and injury. ..
  30. Development of a SARS Coronavirus Vaccine
    Jeffrey Ulmer; Fiscal Year: 2004
    ..An extension of these during the final stages of Specific Aim 4. ..
  31. Yeast-Based High-throughput Assays for Proteases of Category A, B, & C Viruses
    Hong Fang; Fiscal Year: 2009
    ....
  32. Photonic structures for direct ultrasensitive virus detection
    Benjamin L Miller; Fiscal Year: 2012
    ..Applications include the need to monitor emerging viral threats (H5N1 influenza and the SARS virus, for example), and the importance of understanding the long-term implications of nearly undetectable reservoirs ..
  33. Remodeling SARS Coronavirus Genome Regulatory Networks
    Ralph Baric; Fiscal Year: 2004
    ..abstract_text> ..
  34. Protein interactions by analytical ultracentrifugation
    PETER GETTINS; Fiscal Year: 2007
    ..The knowledge gained will be invaluable for design of therapeutics against such infections or in amelioration of diseases associated with aberrant functioning of processes such as blood coagulation. [unreadable] [unreadable] [unreadable]..
  35. Primate enteroviruses transmission in Bangladesh
    Lisa Jones Engel; Fiscal Year: 2007
    ..Ultimately, this data will provide insight into the potential emergence of NHP-borne pathogens and help to deter the spread of emerging zoonotic threats. [unreadable] [unreadable]..
  36. Synthetic Peptide SARS Coronavirus Diagnostic Kit
    Chang Wang; Fiscal Year: 2005
    ..The peptides and kits will be manufactured and characterized with cGMP standards. Large-scale clinical trials will be done at facilities in Asia on extensive collections of SARS positive sera. ..
  37. Analysis of the SARS Virus S glycoproteins
    Paul Bates; Fiscal Year: 2005
    ..Achieving these Specific Aims will not only significantly enhance our understanding of the SARS virus, but will likely aid in the development of vaccines for SARS and in design of therapeutics that block SARS S ..
  38. SARS Coronavirus Reverse Genetics and Pathogenesis
    PAUL MASTERS; Fiscal Year: 2005
    ..It will also generate valuable tools to test antiviral drugs, reveal targets for such drugs, and provide a means to manipulate SARS-CoV for vaccine design. ..
  39. Multiplexed Detection of Bioterror Agents
    Francis Barany; Fiscal Year: 2004
    ..In addition, the LDR/PCR virulence gene test from Aim 2 will be expanded to include the major BT toxin and virulence genes. Once verified, our tests will be validated with clinical samples at the CDC. ..
  40. Analytical Ultracentrifuge Purchase
    Robert Hodges; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  41. Development of Recombinant SARS VLP Vaccines
    Gale Smith; Fiscal Year: 2007
    ..Toxicology studies of the SARS vaccine candidate will be conducted in rabbits. A clinical protocol for a Phase I clinical study will be prepared, and an IND application will be submitted to CBER/FDA. [unreadable] [unreadable]..
  42. Development of Proteosome-adjuvanted nasal SARS vaccines
    David Burt; Fiscal Year: 2004
    ..Formulations inducing optimal levels of SARS virus neutralizing activity in the serum of immunized animals will be further examined in ferret challenge models to ..
  43. Ultrasensitive Bispecific Antibody Assay for SARS Virus
    Mavanur Suresh; Fiscal Year: 2009
    ..to NP (or SG) and HRPO;(c) To optimize an ultrasensitive molecular velcro homosandwich assay of the whole SARS virus on microplates(d) To develop an immunoswab or dipstick assay for samples collected from throat or sputum for ..
  44. Rhabdovirus-based recombinant vaccines against SARS
    Bernhard Dietzschold; Fiscal Year: 2006
    ..Prophylactic immunization is the most effective solution to control SARS in humans and to eradicate the SARS virus reservoirs in animals...
  45. Extramural Research Facilities Construction Bioenvironme
    LANCE PERRYMAN; Fiscal Year: 2005
    ..When completed, the BHRB Expansion will be an integral part of a research complex devoted to safe and efficient study of infectious diseases. ..
  46. Virus-host interactions in replication complex assembly
    David Miller; Fiscal Year: 2009
    ..abstract_text> ..
  47. Development of VLP Vaccines for SARS Prevention
    Richard Compans; Fiscal Year: 2005
    ..systemic immunization via intramuscular injection), with the aim to develop candidate vaccines for large-scale production. ..
  48. Structure of SARS Coronavirus Spike Glycoprotein
    Michael Root; Fiscal Year: 2005
    ..These experiments will explore the structure and function of the SCV spike glycoprotein, essential information for the future development of antiviral therapeutics and vaccines. ..
  49. SARS Coronavirus Interaction with Monocytes Macrophages
    Arlene Collins; Fiscal Year: 2006
    ..abstract_text> ..
  50. Functions of Ub-like Proteins and Proteases
    KEITH WILKINSON; Fiscal Year: 2009
    ..We will determine the enzymatic specificity of the DUB activity exhibited by the SARS virus papain-like protease using the panel of UBL-X reagents...
  51. SARS Vaccine Development
    Jack Gorski; Fiscal Year: 2009
    ..In 2002-2004 SARS was a global public health problem, and it remains so today as the viral reservoir persists in the environment for possible re-emergence. ..
  52. Immunostimulatory Patch To Enhance Biodefense Vaccines
    Larry Ellingsworth; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  53. Antivirals directed to novel targets in the virus-coded proteinase of adenovirus
    WALTER MANGEL; Fiscal Year: 2007
    ..abstract_text> ..
  54. Developing Vaccine Candidates for the SARS Coronavirus
    Ralph Baric; Fiscal Year: 2009
    ....
  55. 2-5A Antisense for Degradation of SARS Coronavirus RNA
    Hagen Cramer; Fiscal Year: 2004
    ..We propose to develop an effective and marketable 2-5A antisense drug for the treatment of SARS infection. ..
  56. Therapeutic and Diagnostic Antibodies Against SARS
    EILEEN TOZER; Fiscal Year: 2006
    ..Specific Aim 3: Test efficacy of primary lead candidates for neutralization of SARS in novel in vitro infection model system. ..
  57. Development of a plant-derived virus-like particle vaccine against SARS-CoV
    BRENDA HOGUE; Fiscal Year: 2009
    ..The long term goal of this project is to develop a novel subunit vaccine that elicits protection against SARS coronavirus. ..
  58. Identification of SARS Antigens for Serodiagnosis
    Andrew Levin; Fiscal Year: 2005
    ..The target antigen will be the SARS virus spike glycoprotein, a potent immunogen in other coronaviruses...
  59. Rapid Detection of Agents that Cause Respiratory Disease
    JAMES PRUDENT; Fiscal Year: 2004
    ..years that targets 15 agents that cause infections in the respiratory tract that may be misdiagnosed for the SARS virus. Our preliminary targets have genomes constructed from RNA or DNA and therefore the test will be required to ..
  60. Virion Solvent Treatment for SARS Vaccine Preparation
    Moiz Kitabwalla; Fiscal Year: 2004
    ..Specific Aims are (1) Optimize proprietary solvent treatment method for SARS virus based on previous virus experience and evaluate native viral protein structure and viral envelope changes post ..
  61. STUDIES OF A SARS-CORONAVIRUS RECEPTOR
    Michael Farzan; Fiscal Year: 2008
    ..These studies will provide a comprehensive description of the S-protein/ACE2 association and of the role of ACE2 in S-protein-mediated fusion. ..
  62. DESIGN AND SYNTHESIS OF NONPEPTIDE PROTEASE INHIBITORS
    Arun K Ghosh; Fiscal Year: 2010
    ..This research integrates organic synthesis, protein-ligand x-ray crystallography, molecular modeling and in-depth virus and cell-biological studies to design the next generation of HIV-1 protease inhibitors. ..
  63. DEVELOPMENT OF LIGAND ASSISTED ASYMMETRIC SYNTHESES
    Arun Ghosh; Fiscal Year: 2001
    ..Besides the broad range of scope and generality, this line of research will provide excellent opportunities to teach and train graduate and undergraduate students in the laboratory. ..
  64. Assembly and release of the SARS coronavirus
    Carolyn Machamer; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..