bacteriophage p22

Summary

Summary: A species of temperate bacteriophage in the genus P22-like Viruses, family PODOVIRIDAE, that infects Salmonella species. The genome consists of double-stranded DNA, terminally redundant, and circularly permuted.

Top Publications

  1. pmc Phase variation controls expression of Salmonella lipopolysaccharide modification genes by a DNA methylation-dependent mechanism
    S E Broadbent
    Centre for Immunology and Infection, Hull York Medical School and the Department of Biology, University of York, York, UK
    Mol Microbiol 77:337-53. 2010
  2. pmc Genomic analysis of bacteriophage epsilon 34 of Salmonella enterica serovar Anatum (15+)
    Robert Villafane
    Ponce School of Medicine, Department of Microbiology, Ponce, Puerto Rico 00732, USA
    BMC Microbiol 8:227. 2008
  3. ncbi Evolution of mosaically related tailed bacteriophage genomes seen through the lens of phage P22 virion assembly
    Sherwood R Casjens
    Pathology Department, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Virology 411:393-415. 2011
  4. pmc Decoding bacteriophage P22 assembly: identification of two charged residues in scaffolding protein responsible for coat protein interaction
    Juliana R Cortines
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    Virology 421:1-11. 2011
  5. pmc Corrected sequence of the bacteriophage p22 genome
    Marisa L Pedulla
    Pittsburgh Bacteriophage Institute Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Bacteriol 185:1475-7. 2003
  6. ncbi Crystal structure of Escherichia coli phage HK620 tailspike: podoviral tailspike endoglycosidase modules are evolutionarily related
    Stefanie Barbirz
    Physikalische Biochemie, Universitat Potsdam, Karl Liebknecht Str 24 25, 14476 Golm, Germany
    Mol Microbiol 69:303-16. 2008
  7. pmc Tailspike interactions with lipopolysaccharide effect DNA ejection from phage P22 particles in vitro
    Dorothee Andres
    Department of Biochemistry and Biology, Laboratory of Physical Biochemistry, University of Potsdam, 14476 Potsdam Golm, Germany
    J Biol Chem 285:36768-75. 2010
  8. ncbi Structure of phage P22 cell envelope–penetrating needle
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    Nat Struct Mol Biol 14:1221-6. 2007
  9. ncbi Carbohydrate binding of Salmonella phage P22 tailspike protein and its role during host cell infection
    Dorothee Andres
    Physikalische Biochemie, Universitat Potsdam, Karl Liebknecht Strasse 24 25, 14476 Golm, Germany
    Biochem Soc Trans 38:1386-9. 2010
  10. ncbi Extension to 2268 atoms of direct methods in the ab initio determination of the unknown structure of bacteriophage P22 lysozyme
    Blaine H M Mooers
    Institute of Molecular Biology, Howard Hughes Medical Institute and Department of Physics, 1229 University of Oregon, Eugene, OR 97403 1229, USA
    Acta Crystallogr D Biol Crystallogr 62:165-76. 2006

Scientific Experts

Detail Information

Publications148 found, 100 shown here

  1. pmc Phase variation controls expression of Salmonella lipopolysaccharide modification genes by a DNA methylation-dependent mechanism
    S E Broadbent
    Centre for Immunology and Infection, Hull York Medical School and the Department of Biology, University of York, York, UK
    Mol Microbiol 77:337-53. 2010
    ..Most Salmonella gtr operons share the key regulatory elements that are identified here as essential for this epigenetic phase variation...
  2. pmc Genomic analysis of bacteriophage epsilon 34 of Salmonella enterica serovar Anatum (15+)
    Robert Villafane
    Ponce School of Medicine, Department of Microbiology, Ponce, Puerto Rico 00732, USA
    BMC Microbiol 8:227. 2008
    ....
  3. ncbi Evolution of mosaically related tailed bacteriophage genomes seen through the lens of phage P22 virion assembly
    Sherwood R Casjens
    Pathology Department, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Virology 411:393-415. 2011
    ..Here we review and examine the mosaicism within fifty-seven clusters of virion assembly genes from bacteriophage P22 and its "close" relatives...
  4. pmc Decoding bacteriophage P22 assembly: identification of two charged residues in scaffolding protein responsible for coat protein interaction
    Juliana R Cortines
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    Virology 421:1-11. 2011
    ..The 303 amino acid bacteriophage P22 scaffolding protein is mostly helical, and its C-terminal helix-turn-helix (HTH) domain binds to the coat ..
  5. pmc Corrected sequence of the bacteriophage p22 genome
    Marisa L Pedulla
    Pittsburgh Bacteriophage Institute Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Bacteriol 185:1475-7. 2003
    We report the first accurate genome sequence for bacteriophage P22, correcting a 0.14% error rate in previously determined sequences...
  6. ncbi Crystal structure of Escherichia coli phage HK620 tailspike: podoviral tailspike endoglycosidase modules are evolutionarily related
    Stefanie Barbirz
    Physikalische Biochemie, Universitat Potsdam, Karl Liebknecht Str 24 25, 14476 Golm, Germany
    Mol Microbiol 69:303-16. 2008
    ..We propose that the tailspike genes of P22, Sf6 and HK620 have a common precursor and are not mosaics of unrelated gene fragments...
  7. pmc Tailspike interactions with lipopolysaccharide effect DNA ejection from phage P22 particles in vitro
    Dorothee Andres
    Department of Biochemistry and Biology, Laboratory of Physical Biochemistry, University of Potsdam, 14476 Potsdam Golm, Germany
    J Biol Chem 285:36768-75. 2010
    Initial attachment of bacteriophage P22 to the Salmonella host cell is known to be mediated by interactions between lipopolysaccharide (LPS) and the phage tailspike proteins (TSP), but the events that subsequently lead to DNA injection ..
  8. ncbi Structure of phage P22 cell envelope–penetrating needle
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    Nat Struct Mol Biol 14:1221-6. 2007
    b>Bacteriophage P22 infects Salmonella enterica by injecting its genetic material through the cell envelope. During infection, a specialized tail needle, gp26, is injected into the host, likely piercing a hole in the host cell envelope...
  9. ncbi Carbohydrate binding of Salmonella phage P22 tailspike protein and its role during host cell infection
    Dorothee Andres
    Physikalische Biochemie, Universitat Potsdam, Karl Liebknecht Strasse 24 25, 14476 Golm, Germany
    Biochem Soc Trans 38:1386-9. 2010
    TSPs (tailspike proteins) are essential infection organelles of bacteriophage P22. Upon infection, P22TSP binds to and cleaves the O-antigen moiety of the LPS (lipopolysaccharide) of its Salmonella host...
  10. ncbi Extension to 2268 atoms of direct methods in the ab initio determination of the unknown structure of bacteriophage P22 lysozyme
    Blaine H M Mooers
    Institute of Molecular Biology, Howard Hughes Medical Institute and Department of Physics, 1229 University of Oregon, Eugene, OR 97403 1229, USA
    Acta Crystallogr D Biol Crystallogr 62:165-76. 2006
    The X-ray crystal structure of the previously unknown bacteriophage P22 lysozyme, the product of gene 19, has been determined ab initio by direct methods using the program SIR2002...
  11. pmc Detection of intermediates and kinetic control during assembly of bacteriophage P22 procapsid
    Roman Tuma
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Mol Biol 381:1395-406. 2008
    b>Bacteriophage P22 serves as a model for the assembly and maturation of other icosahedral double-stranded DNA viruses...
  12. pmc Unraveling the role of the C-terminal helix turn helix of the coat-binding domain of bacteriophage P22 scaffolding protein
    G Pauline Padilla-Meier
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA
    J Biol Chem 287:33766-80. 2012
    ..In bacteriophage P22, the association of scaffolding and coat proteins is mediated mainly by ionic interactions...
  13. pmc Subunit conformations and assembly states of a DNA-translocating motor: the terminase of bacteriophage P22
    Daniel Nemecek
    School of Biological Sciences, University of Missouri Kansas City, 5100 Rockhill Road, Kansas City, MO 64110, USA
    J Mol Biol 374:817-36. 2007
    b>Bacteriophage P22, a podovirus infecting strains of Salmonella typhimurium, packages a 42-kbp genome using a headful mechanism...
  14. pmc Determinants of bacteriophage P22 polyhead formation: the role of coat protein flexibility in conformational switching
    Margaret M Suhanovsky
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA
    Mol Microbiol 77:1568-82. 2010
    We have investigated determinants of polyhead formation in bacteriophage P22 in order to understand the molecular mechanism by which coat protein assembly goes astray...
  15. ncbi Determination of antioxidant capacity using the biological system bacteriophage P22/bacterium Salmonella typhimurium
    Maria S Gião
    Escola Superior de Biotecnologia, Universidade Catolica Portuguesa, Rua Dr Antonio Bernardino de Almeida, Porto, Portugal
    J Agric Food Chem 57:22-5. 2009
    ..The P22/Salmonella typhimurium model system thus proved to be useful in the assessment of antioxidant capacity in vivo, at least using those two alternative model antioxidants...
  16. pmc Foldon-guided self-assembly of ultra-stable protein fibers
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Protein Sci 17:1475-85. 2008
    ..This engineered protein fiber was constructed on the molecular framework of bacteriophage P22 tail needle gp26, by fusing its helical core to the foldon domain of phage T4 fibritin...
  17. pmc The P22 tail machine at subnanometer resolution reveals the architecture of an infection conduit
    Gabriel C Lander
    National Resource for Automated Molecular Microscopy, The Scripps Institute, La Jolla, CA 92037, USA
    Structure 17:789-99. 2009
    The portal channel is a key component in the life cycle of bacteriophages and herpesviruses. The bacteriophage P22 portal is a 1 megadalton dodecameric oligomer of gp1 that plays key roles in capsid assembly, DNA packaging, assembly of ..
  18. pmc Determination of stoichiometry and conformational changes in the first step of the P22 tail assembly
    Kristina Lorenzen
    Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research, Utrecht Institute for Chemistry, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    J Mol Biol 379:385-96. 2008
    ..In this study, we have investigated the stoichiometry of the in vitro-assembled portal protein of bacteriophage P22 and characterized its association with the tail factor gp4...
  19. pmc 'Let the phage do the work': using the phage P22 coat protein structures as a framework to understand its folding and assembly mutants
    Carolyn M Teschke
    Department of Molecular and Cell Biology, 91 N Eagleville Rd, U 3125, University of Connecticut, Storrs, CT 06269 3125, USA
    Virology 401:119-30. 2010
    ..Here we highlight the pseudo-atomic structures of phage P22 coat protein and rationalize several decades of data about P22 coat protein folding, assembly and maturation generated from a combination of genetics and biochemistry...
  20. pmc A conformational switch in bacteriophage p22 portal protein primes genome injection
    Hongjin Zheng
    Department of Biochemistry, University of Washington, 1705 NE Pacific Street, Seattle, WA 98195, USA
    Mol Cell 29:376-83. 2008
    ..We have used electron cryomicroscopy to determine the structure of bacteriophage P22 portal protein in both the procapsid and mature capsid conformations...
  21. pmc Structural plasticity of the phage P22 tail needle gp26 probed with xenon gas
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Protein Sci 18:537-48. 2009
    The tail needle, gp26, is a highly stable homo-trimeric fiber found in the tail apparatus of bacteriophage P22. In the mature virion, gp26 is responsible for plugging the DNA exit channel, and likely plays an important role in ..
  22. ncbi Domain study of bacteriophage p22 coat protein and characterization of the capsid lattice transformation by hydrogen/deuterium exchange
    Sebyung Kang
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Mol Biol 347:935-48. 2005
    ..The dsDNA bacteriophage P22 is used as a model system to study the assembly and maturation of icosahedral dsDNA viruses...
  23. ncbi Preliminary crystallographic analysis of the bacteriophage P22 portal protein
    Gino Cingolani
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Struct Biol 139:46-54. 2002
    ..We have studied the portal protein of bacteriophage P22, which is the largest connector characterized among the tailed bacteriophages...
  24. ncbi Role of gene 10 protein in the hierarchical assembly of the bacteriophage P22 portal vertex structure
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E Adams Street, Syracuse, New York 13210, USA
    Biochemistry 46:8776-84. 2007
    ..The assembled portal vertex structure represents both a membrane-binding and penetrating device as well as a plug that retains the pressurized phage DNA inside the capsid...
  25. ncbi Structural transformations accompanying the assembly of bacteriophage P22 portal protein rings in vitro
    S D Moore
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 276:6779-88. 2001
    The Salmonella typhimurium bacteriophage P22 assembles an icosahedral capsid precursor called a procapsid. The oligomeric portal protein ring, located at one vertex, comprises the conduit for DNA entry and exit...
  26. pmc Binding of the bacteriophage P22 N-peptide to the boxB RNA motif studied by molecular dynamics simulations
    Ranjit P Bahadur
    School of Engineering and Science, Jacobs University Bremen, Bremen, Germany
    Biophys J 97:3139-49. 2009
    ..It indicates that electrostatic interactions near RNA molecules can lead to a shift in the equilibrium toward the bound form of an interacting partner before it enters the binding pocket...
  27. ncbi Coat protein fold and maturation transition of bacteriophage P22 seen at subnanometer resolutions
    Wen Jiang
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nat Struct Biol 10:131-5. 2003
    b>Bacteriophage P22 is a prototypical biological machine used for studying protein complex assembly and capsid maturation. Using cryo-EM, we solved the structures of P22 before and after the capsid maturation at 8.5 A and 9...
  28. pmc Assembly architecture and DNA binding of the bacteriophage P22 terminase small subunit
    Daniel Nemecek
    School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110 2499, USA
    J Mol Biol 383:494-501. 2008
    Morphogenesis of bacteriophage P22 involves the packaging of double-stranded DNA into a preassembled procapsid...
  29. ncbi Morphing molecular specificities between Arm-peptide and NUT-RNA in the antitermination complexes of bacteriophages lambda and P22
    Naomi C Franklin
    Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112 0840, USA
    Mol Microbiol 52:815-22. 2004
    ..Intermediate sequences may show no function with either BOX-B, or weak function with both BOX-B(lambda) and BOX-B(22), the latter suggesting possible evolutionary paths for specificity shifts...
  30. pmc Nucleotide sequence of the head assembly gene cluster of bacteriophage L and decoration protein characterization
    Eddie B Gilcrease
    Division of Cell Biology and Immunology, Department of Pathology, University of Utah Medical School, Salt Lake City, UT 84132, USA
    J Bacteriol 187:2050-7. 2005
    The temperate Salmonella enterica bacteriophage L is a close relative of the very well studied bacteriophage P22. In this study we show that the L procapsid assembly and DNA packaging genes, which encode terminase, portal, scaffold, and ..
  31. ncbi An intersubunit active site between supercoiled parallel beta helices in the trimeric tailspike endorhamnosidase of Shigella flexneri Phage Sf6
    Jürgen J Müller
    Max Delbruck Centrum fur Molekulare Medizin, Robert Rossle Str 10, 13125 Berlin, Germany
    Structure 16:766-75. 2008
    ..Sf6 TSP may serve as an example for the evolution of different host specificities on a similar general architecture...
  32. ncbi Cryo-EM asymmetric reconstruction of bacteriophage P22 reveals organization of its DNA packaging and infecting machinery
    Juan Chang
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Structure 14:1073-82. 2006
    ..particle cryo-electron microscopy and asymmetric 3D reconstruction reveal the organization of the complete bacteriophage P22 virion, including the protein channel through which DNA is first packaged and later ejected...
  33. pmc Interactions of phage P22 tails with their cellular receptor, Salmonella O-antigen polysaccharide
    U Baxa
    Universtät Regensburg, Institut fur Biophysik und Physikalische Biochemie, Germany
    Biophys J 71:2040-8. 1996
    b>Bacteriophage P22 binds to its cell surface receptor, the repetitive O-antigen structure in Salmonella lipopolysaccharide, by its six homotrimeric tailspikes...
  34. ncbi Separating lysozyme from bacteriophage P22 in two-phase aqueous micellar systems
    Daniel T Kamei
    Department of Chemical Engineering, Room 66 444, MIT, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Biotechnol Bioeng 80:233-6. 2002
    ..net positively charged protein, lysozyme, in the micelle-rich phase from 75 to 95%, while still maintaining approximately the same yield of a model net negatively charged virus, bacteriophage P22, in the micelle-poor phase (97% vs. 98%).
  35. ncbi Crystal structure of P22 tailspike protein: interdigitated subunits in a thermostable trimer
    S Steinbacher
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Martinsried, Germany
    Science 265:383-6. 1994
    ..The interdigitation of the polypeptide chains at the carboxyl termini is important to protrimer formation in the folding pathway and to thermostability of the mature protein...
  36. ncbi Identification of subunit-subunit interactions in bacteriophage P22 procapsids by chemical cross-linking and mass spectrometry
    Sebyung Kang
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Proteome Res 5:370-7. 2006
    ..The dsDNA bacteriophage P22 is used as a model system to study the assembly and maturation of icosahedral dsDNA viruses...
  37. ncbi Binding-induced stabilization and assembly of the phage P22 tail accessory factor gp4
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750, E Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 363:558-76. 2006
    To infect and replicate, bacteriophage P22 injects its 43 kbp genome across the cell wall of Salmonella enterica serovar Typhimurium...
  38. pmc Crystal structure of phage P22 tailspike protein complexed with Salmonella sp. O-antigen receptors
    S Steinbacher
    Abteilung Strukturforschung, Max Planck Institut fur Biochemie, Martinsried, Germany
    Proc Natl Acad Sci U S A 93:10584-8. 1996
    ..Kinetics of binding and cleavage suggest a role of the receptor destroying endorhamnosidase activity primarily for detachment of newly assembled phages...
  39. ncbi Phage P22 tailspike protein: crystal structure of the head-binding domain at 2.3 A, fully refined structure of the endorhamnosidase at 1.56 A resolution, and the molecular basis of O-antigen recognition and cleavage
    S Steinbacher
    Max Planck Institut fur Biochemie, Abteilung für Strukturforschung, Martinsried, Germany
    J Mol Biol 267:865-80. 1997
    ..In the final step of bacteriophage P22 assembly six homotrimeric tailspike molecules are non-covalently attached to the DNA injection apparatus, ..
  40. ncbi Homology between two different Salmonella phages: Salmonella enterica serovar Typhimurium phage P22 and Salmonella enterica serovar Anatum var. 15 + phageepsilon34
    Clari J Salgado
    UCC School of Medicine, Bayamon, Puerto Rico 00960
    Virus Genes 29:87-98. 2004
    ..Significantly this report suggests a general structural motif for part of the TSP of phages and suggests that a more detailed comparative analysis of these TSPs is warranted...
  41. ncbi DNA packaging and delivery machines in tailed bacteriophages
    John E Johnson
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Opin Struct Biol 17:237-43. 2007
    ....
  42. ncbi The tailspike protein of Shigella phage Sf6. A structural homolog of Salmonella phage P22 tailspike protein without sequence similarity in the beta-helix domain
    Alexander Freiberg
    Physikalische Biochemie, Universitat Potsdam, Karl Liebknecht Strasse 24 25, D 14476 Golm, Germany
    J Biol Chem 278:1542-8. 2003
    ..The observations strongly suggest that the tailspike of phage Sf6 is a trimeric parallel beta-helix protein with high structural similarity to its functional homolog from phage P22...
  43. pmc The interdigitated beta-helix domain of the P22 tailspike protein acts as a molecular clamp in trimer stabilization
    Jason F Kreisberg
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Protein Sci 11:820-30. 2002
    ..This interdigitated beta-helix appears to function as a molecular clamp insuring thermostable subunit association in the native trimer...
  44. ncbi Molecular genetics of bacteriophage P22 scaffolding protein's functional domains
    Peter R Weigele
    Department of Pathology, University of Utah School of Medicine, 50 North 1900 East, Salt Lake City, UT 84132, USA
    J Mol Biol 348:831-44. 2005
    The assembly intermediates of the Salmonella bacteriophage P22 are well defined but the molecular interactions between the subunits that participate in its assembly are not...
  45. ncbi Nucleotide sequence of coliphage HK620 and the evolution of lambdoid phages
    A J Clark
    Department of Molecular and Cell Biology, Division of Life Sciences, MS74 157, Berkeley, CA 94720, USA
    J Mol Biol 311:657-79. 2001
    ..We propose that these boundary sequences are foci of genetic recombination that serve to assort the modules and make the metabolic domain highly mosaic genetically...
  46. ncbi Development of a novel method of lytic phage delivery by use of a bacteriophage P22 site-specific recombination system
    Ratree Platt
    Department of Veterinary Microbiology and Preventive Medicine, 1802 Elwood Drive, Veterinary Medical Research Institute, Building 6, Iowa State University, Ames 50011, USA
    FEMS Microbiol Lett 223:259-65. 2003
    ..accomplished by development of a plasmid-based system utilizing the site-specific recombination machinery of bacteriophage P22 to integrate DNA constructs into the host chromosome...
  47. ncbi The DNA site utilized by bacteriophage P22 for initiation of DNA packaging
    Hongyu Wu
    Department of Pathology, University of Utah Medical Center, Salt Lake City 84132, USA
    Mol Microbiol 45:1631-46. 2002
    ..Viruses like bacteriophage P22, which have partially circularly permuted, double-stranded virion DNAs, encapsidate DNA through processive ..
  48. pmc Crystallogenesis of bacteriophage P22 tail accessory factor gp26 at acidic and neutral pH
    Gino Cingolani
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:477-82. 2006
    ..These crystals diffract X-rays to beyond 2.0 angstroms resolution. Structural analysis of gp26 crystallized at acidic, neutral and alkaline pH is in progress...
  49. pmc An elongated spine of buried core residues necessary for in vivo folding of the parallel beta-helix of P22 tailspike adhesin
    Ryan Simkovsky
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 103:3575-80. 2006
    ..Such a stepwise folding pathway offers a new target for drug design against this class of microbial virulence factors...
  50. ncbi Characterization of the protrimer intermediate in the folding pathway of the interdigitated beta-helix tailspike protein
    Christopher B Benton
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 41:5093-103. 2002
    ..This late step may confer the detergent-resistance, protease-resistance, and thermostability of the native trimer...
  51. pmc Cotranslational folding promotes beta-helix formation and avoids aggregation in vivo
    Michael S Evans
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA
    J Mol Biol 383:683-92. 2008
    ....
  52. pmc Three-dimensional structure of the bacteriophage P22 tail machine
    Liang Tang
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 24:2087-95. 2005
    The tail of the bacteriophage P22 is composed of multiple protein components and integrates various biological functions that are crucial to the assembly and infection of the phage...
  53. pmc GroEL/S substrate specificity based on substrate unfolding propensity
    Kristin N Parent
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA
    Cell Stress Chaperones 12:20-32. 2007
    ..Our data also suggest that GroEL/S can be induced by increasing the population of unfolding intermediates...
  54. ncbi Quantitative analysis of multi-component spherical virus assembly: scaffolding protein contributes to the global stability of phage P22 procapsids
    Kristin N Parent
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3125, USA
    J Mol Biol 359:1097-106. 2006
    ..When the relative concentration is very high, too many nuclei form, leading to kinetically trapped assembly intermediates...
  55. ncbi Highly discriminatory binding of capsid-cementing proteins in bacteriophage L
    Liang Tang
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Structure 14:837-45. 2006
    ..Sequence analysis shows striking similarity between the C-terminal portion of phage L Dec protein and five regions in the long tail fiber of a T4-like phage, suggesting functional parallelism between them...
  56. ncbi Chemical chaperone-mediated protein folding: stabilization of P22 tailspike folding intermediates by glycerol
    Rajesh Mishra
    Department of Biochemistry and Biology, Potsdam University, D 14476 Potsdam Golm, Germany
    Biol Chem 388:797-804. 2007
    ..Our results suggest that the chemical chaperone effect of polyols observed in the folding of large proteins is due to preferential hydration favoring structure formation in folding intermediates...
  57. pmc Protein folding failure sets high-temperature limit on growth of phage P22 in Salmonella enterica serovar Typhimurium
    Welkin H Pope
    Massachusetts Institute of Technology, 77 Massachusetts Ave 68 330, Cambridge, MA 02139, USA
    Appl Environ Microbiol 70:4840-7. 2004
    ..Thus, we believe that the folding pathway of the tailspike adhesin sets the high-temperature limit for P22 formation in Salmonella serovar Typhimurium...
  58. ncbi Impact of in vitro assembly defects on in vivo function of the phage P22 portal
    Ying Sun
    Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri Kansas City, 5100 Rockhill Road, Kansas City, MO 64110, USA
    Virology 365:336-45. 2007
    ..Both C153S and C173S portals function at levels close to wt. The results suggest that C283S and C516S mutations may be exploited for improved characterization of the folding and assembly pathway of P22 portal protein...
  59. ncbi Bacteriophage p22 portal vertex formation in vivo
    Sean D Moore
    Department of Microbiology BBRB 416 6, University of Alabama at Birmingham, 845 19th St South, Birmingham, AL 35294, USA
    J Mol Biol 315:975-94. 2002
    ..Previous studies have suggested that the portal protein of bacteriophage P22 is not essential for shell assembly; however, when assembled in the absence of functional portal protein, ..
  60. ncbi Mud-P22
    Jenny A Crain
    Department of Biology, Center for Microbial Sciences, San Diego State University, San Diego, CA, USA
    Methods Enzymol 421:249-59. 2007
    ..Although Mud-P22 has not yet been used in other species, this approach should be applicable in a variety of other bacteria as well...
  61. doi Development of bacteriophage p22 as a platform for molecular display: genetic and chemical modifications of the procapsid exterior surface
    Sebyung Kang
    Department of Microbiology, University of Alabama at Birmingham, BBRB 416 845 19th St South, Birmingham, AL 35294 2170, USA
    Chembiochem 9:514-8. 2008
  62. ncbi Rapid unfolding of a domain populates an aggregation-prone intermediate that can be recognized by GroEL
    Shannon M Doyle
    Department of Molecular and Cell Biology, University of Connecticut, 91 N Eagleville Road, Storrs, CT 06269 3125, USA
    J Mol Biol 332:937-51. 2003
    ..Thus, the tsf coat proteins aggregate in vivo because of an increased propensity to populate this unfolding intermediate...
  63. ncbi The structure of an infectious P22 virion shows the signal for headful DNA packaging
    Gabriel C Lander
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 312:1791-5. 2006
    ..We propose that the tight dsDNA spooling activates the switch that signals the headful chromosome packing density to the particle exterior...
  64. pmc A P22 scaffold protein mutation increases the robustness of head assembly in the presence of excess portal protein
    Sean D Moore
    Department of Microbiology, University of Alabama at Birmingham, 845 South 19th Street, Birmingham, AL 35295, USA
    J Virol 76:10245-55. 2002
    ....
  65. pmc Role for cysteine residues in the in vivo folding and assembly of the phage P22 tailspike
    C Haase-Pettingell
    Department of Biology, Masschusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Protein Sci 10:397-410. 2001
    ..The formation or reduction of the transient interchain disulfide bonds in the protrimer may be the locus of these kinetic functions...
  66. ncbi A concerted mechanism for the suppression of a folding defect through interactions with chaperones
    Shannon M Doyle
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 3125, USA
    J Biol Chem 279:17473-82. 2004
    Specific amino acid substitutions confer a temperature-sensitive-folding (tsf) phenotype to bacteriophage P22 coat protein. Additional amino acid substitutions, called suppressor substitutions (su), relieve the tsf phenotype...
  67. pmc Pressure dissociation studies provide insight into oligomerization competence of temperature-sensitive folding mutants of P22 tailspike
    Brian G Lefebvre
    Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA
    Protein Sci 13:1538-46. 2004
    Several temperature-sensitive folding (tsf) mutants of the tailspike protein from bacteriophage P22 have been found to fold with lower efficiency than the wild-type sequence, even at lowered temperatures...
  68. ncbi Hydrogen-deuterium exchange as a probe of folding and assembly in viral capsids
    R Tuma
    Department of Microbiology, The University of Alabama at Birmingham, Birmingham, AL, 35294-2170, USA
    J Mol Biol 306:389-96. 2001
    ..The capsid expansion (maturation) accompanying DNA packaging in the dsDNA bacteriophage P22 represents an experimentally accessible case of such a transformation...
  69. pmc Penton release from P22 heat-expanded capsids suggests importance of stabilizing penton-hexon interactions during capsid maturation
    Carolyn M Teschke
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA
    Biophys J 84:2585-92. 2003
    ..In this work, we examine bacteriophage P22 morphogenesis by comparing three-dimensional structures of capsids expanded both in vitro by heat treatment ..
  70. ncbi Folding of phage P22 coat protein monomers: kinetic and thermodynamic properties
    Eric Anderson
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3125, USA
    Virology 313:184-97. 2003
    ..Additionally, two native states were identified, suggesting that the several conformers required to assemble an icosahedral capsid may be found in solution before assembly ensues...
  71. ncbi Structural roles of subunit cysteines in the folding and assembly of the DNA packaging machine (portal) of bacteriophage P22
    Arantxa Rodriguez-Casado
    Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri Kansas City, 64110 2499, USA
    Biochemistry 42:3437-45. 2003
    The DNA packaging machine (portal assembly) of bacteriophage P22 is constructed from 12 copies of a multidomain 725-residue subunit comprising a complex alpha/beta fold...
  72. ncbi Structure of bacteriophage P22 portal protein in relation to assembly: investigation by Raman spectroscopy
    A Rodriguez-Casado
    Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri Kansas City, Kansas City, Missouri 64110 2499, USA
    Biochemistry 40:13583-91. 2001
    ..This motif is proposed as an evolutionarily conserved domain involved in DNA translocation...
  73. pmc Orientation determination by wavelets matching for 3D reconstruction of very noisy electron microscopic virus images
    Ali Samir Saad
    Department of Biomedical Technology, College of Applied Medical Sciences, King Saud University, P, O, Box, 10219, Riyadh 11433, Kingdom of Saudi Arabia
    BMC Struct Biol 5:5. 2005
    ..In order to speed the retrieval of the best match between projections from a model and real virus particle, a hierarchical correlation matching method is also proposed...
  74. ncbi A second-site suppressor of a folding defect functions via interactions with a chaperone network to improve folding and assembly in vivo
    Kristin N Parent
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3125, USA
    Mol Microbiol 54:1036-50. 2004
    ..Here we report evidence that su substitutions that rescue bacteriophage P22 temperature-sensitive-folding (tsf) coat protein variants function in a novel way...
  75. pmc Consolidating critical binding determinants by noncyclic rearrangement of protein secondary structure
    Ramon K Tabtiang
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 102:2305-9. 2005
    ..Noncyclic rearrangement of secondary structure may allow grouping of critical active-site residues in other proteins and could be a useful tool for protein design and minimization...
  76. ncbi Bacteriophage P22 tail accessory factor GP26 is a long triple-stranded coiled-coil
    Dewan Andrews
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E Adams St, Syracuse, NY 13078, USA
    J Biol Chem 280:5929-33. 2005
    ..By analogy with viral trimeric coiled-coil class I membrane fusion proteins, gp26 may represent the membrane-penetrating device used by the phage to pierce the host outer membrane...
  77. ncbi Nucleotide sequence of the bacteriophage P22 genes required for DNA packaging
    K Eppler
    Department of Cellular, Viral, and Molecular Biology, University of Utah Medical Center, Salt Lake City 84132
    Virology 183:519-38. 1991
    The mechanism of DNA packaging by dsDNA viruses is not well understood in any system. In bacteriophage P22 only five genes are required for successful condensation of DNA within the capsid...
  78. ncbi Arc repressor-operator DNA interactions and contribution of Phe10 to binding specificity
    Lubomír Dostál
    AG Biopolymerspektroskopie, Max Delbrück Centrum für Molekulare Medizin Berlin Buch, Robert Rossle Strasse 10, D 13092 Berlin, Germany
    Biochemistry 44:8387-96. 2005
    Solution properties of Arc repressors (wild-type and F10H variant) from Salmonella bacteriophage P22 and their complexes with operator DNA (Arc-wt-DNA and Arc-F10H-DNA) were characterized by circular dichroism, fluorescence, and Raman ..
  79. ncbi The Shigella flexneri bacteriophage Sf6 tailspike protein (TSP)/endorhamnosidase is related to the bacteriophage P22 TSP and has a motif common to exo- and endoglycanases, and C-5 epimerases
    J E Chua
    Department of Microbiology and Immunology, University of Adelaide, South Australia
    Microbiology 145:1649-59. 1999
    ..an open reading frame (ORF1) of 1872 bp (624 amino acids) bearing amino acid sequence homology to the bacteriophage P22 TSP N-terminal head-binding domain...
  80. pmc Structure of the receptor-binding protein of bacteriophage det7: a podoviral tail spike in a myovirus
    Monika Walter
    Physical Biochemistry, University of Potsdam, Karl Liebknecht Str 24 25, Haus 25, D 14476 Potsdam Golm, Germany
    J Virol 82:2265-73. 2008
    ..6-A resolution, is very similar to that of bacteriophage P22 tail spike...
  81. pmc The isolation and characterization of mutants of the integration host factor (IHF) of Escherichia coli with altered, expanded DNA-binding specificities
    E C Lee
    Department of Microbiology, University of Illinois, Urbana 61801
    EMBO J 11:305-13. 1992
    ..IHF is a sequence-specific DNA-binding protein and bends the DNA when it binds. We have used the bacteriophage P22-based challenge phage selection to isolate suppressor mutants with altered, expanded DNA binding ..
  82. ncbi New structural insights from Raman spectroscopy of proteins and their assemblies
    George J Thomas
    Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri Kansas City, 5100 Rockhill Road, 64110 2499, USA
    Biopolymers 67:214-25. 2002
    ..band for each of the eight cysteines per 666-residue subunit in the trimeric tailspike of icosahedral bacteriophage P22. The Raman sulfhydryl signatures of the wild-type tailspike and eight single-site cysteine to serine mutants ..
  83. ncbi Generation of deletions and duplications using transposons as portable regions of homology with emphasis on mud and Tn10 transposons
    Kelly T Hughes
    Department of Biology, University of Utah, Salt Lake City, UT, USA
    Methods Enzymol 421:51-68. 2007
    ..any region of the Salmonella enterica chromosome between the points of two Mud insertions in a simple bacteriophage P22 transductional cross...
  84. pmc Cryo-reconstructions of P22 polyheads suggest that phage assembly is nucleated by trimeric interactions among coat proteins
    Kristin N Parent
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA
    Phys Biol 7:045004. 2010
    b>Bacteriophage P22 forms an isometric capsid during normal assembly, yet when the coat protein (CP) is altered at a single site, helical structures (polyheads) also form...
  85. ncbi Cell division inhibition gene dicB is regulated by a locus similar to lambdoid bacteriophage immunity loci
    S Bejar
    Centre de Recherches de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France
    Mol Gen Genet 212:11-9. 1988
    ..Sequence comparisons indicate that dicA and dicC are similar to genes c2 and cro respectively of bacteriophage P22, and carry similarly organized tandem operators, indicating a common evolutionary origin for dicAC and P22 ..
  86. ncbi Implementation of p22 viral capsids as nanoplatforms
    Sebyung Kang
    Department of Chemistry and Biochemistry and Center for Bio Inspired Nanomaterials, Montana State University, Bozeman, MT 59717, USA
    Biomacromolecules 11:2804-9. 2010
    ..of viral capsids and generated two morphologically different types of viral nanoplatforms from the bacteriophage P22 capsids...
  87. ncbi Secondary structure switching in Cro protein evolution
    Tracey Newlove
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85701 USA
    Structure 12:569-81. 2004
    We report the solution structure of the Cro protein from bacteriophage P22. Comparisons of its sequence and structure to those of lambda Cro strongly suggest an alpha-to-beta secondary structure switching event during Cro evolution...
  88. pmc P22 coat protein structures reveal a novel mechanism for capsid maturation: stability without auxiliary proteins or chemical crosslinks
    Kristin N Parent
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
    Structure 18:390-401. 2010
    ..3D image reconstruction, and comparative modeling to derive two independent, pseudoatomic models of bacteriophage P22 CP: before and after maturation...
  89. ncbi Review: conformation and folding of novel beta-structural elements in viral fiber proteins: the triple beta-spiral and triple beta-helix
    Anna Mitraki
    Institut de Biologie Structurale CEA CNRS UJF, Grenoble, France
    J Struct Biol 137:236-47. 2002
    ..Elongated enzymes such as pectate lyase and the bacteriophage P22 tailspike protein contain single-stranded beta-helices...
  90. pmc An automated procedure for detecting protein folds from sub-nanometer resolution electron density
    Reza Khayat
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 170:513-21. 2010
    ..The identified folds in the last three examples can be used as templates for comparative modeling of the bacteriophage P22 tail-machine (a 3MDa complex composed of 39 protein subunits).
  91. pmc Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication
    C Hatt
    Department of Microbiology, Faculty of Medicine University of Southampton, Southampton General Hospital, UK
    Nucleic Acids Res 16:4053-67. 1988
    ..Homology of the predicted polypeptide product of an open reading frame to the E. coli dnaB protein and the analogous product of gene 12 of bacteriophage P22 is described.
  92. pmc Peering down the barrel of a bacteriophage portal: the genome packaging and release valve in p22
    Jinghua Tang
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093 0378, USA
    Structure 19:496-502. 2011
    ....
  93. pmc Engineering nanocages with polyglutamate domains for coupling to hydroxyapatite biomaterials and allograft bone
    Bonnie K Culpepper
    Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Biomaterials 34:2455-62. 2013
    ..Taken together these results establish a new mechanism for the directed coupling of nanocage drug delivery systems to a variety of HA-containing materials commonly used in diverse bone therapies...
  94. ncbi Fabrication of ordered nanostructures of sulfide nanocrystal assemblies over self-assembled genetically engineered P22 coat protein
    Liming Shen
    Center for Materials for Information Technology, University of Alabama, Tuscaloosa, Alabama 35487, USA
    J Am Chem Soc 132:17354-7. 2010
    ....
  95. ncbi Directed self-assembly of CdS quantum dots on bacteriophage P22 coat protein templates
    Anup Kale
    Centre for Materials for Information Technology, The University of Alabama, Tuscaloosa 35487, AL, USA
    Nanotechnology 24:045603. 2013
    ..The bacteriophage P22 shell is comprised of hexameric and pentameric clusters of subunits known as capsomeres...
  96. ncbi Structure of the coat protein-binding domain of the scaffolding protein from a double-stranded DNA virus
    Y Sun
    Comprehensive Cancer Center, Birmingham, AL, 35294, USA
    J Mol Biol 297:1195-202. 2000
    ..In both bacteriophage P22 and the herpesviruses the extreme scaffold C terminus is highly charged, is predicted to be an amphipathic ..
  97. ncbi Influence of bacteriophage P22 on the inflammatory mediator gene expression in chicken macrophage HD11 cells infected with Salmonella Typhimurium
    Juhee Ahn
    Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, Gangwon, South Korea
    FEMS Microbiol Lett 352:11-7. 2014
    ..mediator genes was determined in ST(P22-) - and ST(P22+) -infected HD11 cells treated with and without bacteriophage P22 at 1 and 24 hpi using quantitative RT-PCR...
  98. pmc Alteration of T4 lysozyme structure by second-site reversion of deleterious mutations
    A R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester 01655, USA
    Protein Sci 6:2418-25. 1997
    ..Some variants of proteins bearing primary substitutions at the positions of Asp 20 and Ala 98 are inferred to have significantly altered structures...
  99. ncbi Biophysical properties of regions flanking the bHLH-Zip motif in the p22 Max protein
    Sharon E Pursglove
    Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden
    Biochem Biophys Res Commun 323:750-9. 2004
    ..Thus, the previously observed functionalities of these Max regions in DNA binding, phosphorylation, and apoptosis are suggested to be linked to structural properties...
  100. ncbi Cloning and characterization of a gene encoding 22 kDa functional protein of bacteriophage MB78
    Lalita Gupta
    Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, U P, India
    J Biochem Mol Biol 38:161-6. 2005
    ..3'end of the fragment has high sequence homology to the EaA and EaI proteins of bacteriophage P22, a close relative of MB78 phage.
  101. pmc Polyhead formation in phage P22 pinpoints a region in coat protein required for conformational switching
    Kristin N Parent
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA
    Mol Microbiol 65:1300-10. 2007
    ..Our results suggest that a previously identified surface-exposed loop in coat protein is critical in conformational switching of subunits during both procapsid assembly and maturation...

Research Grants32

  1. STRUCTURAL STUDIES OF NUCLEOPROTEIN COMPLEXES
    Edward H Egelman; Fiscal Year: 2012
    ..The proposed focus is on capsid proteins from bacteriophage P22, Infectious Bursal Disease Virus, and Murine Leukemia virus...
  2. Structures of the portal vertex in dsDNA viruses and virus-infected cells
    Wah Chiu; Fiscal Year: 2011
    ..will be applied to the Herpes simplex virus type 1 (HSV-1) along with a similar and simpler dsDNA virus, bacteriophage P22 of Salmonella as a testbed for methodology development and testing...
  3. Engineered virus-like nanoparticles targeting immunity needed for pan flu vaccine
    Allen G Harmsen; Fiscal Year: 2013
    ..We will use the VLP, derived from the bacteriophage P22, to encapsulate NP fragments (and/or csHA) selectively on the interior and display csHA (and/or NP fragments)..
  4. Electron cryo-microscopy of phage P22
    Kristin N Parent; Fiscal Year: 2010
    ..b>Bacteriophage P22 shares features of assembly and a high degree of structural conservation among the major CPs of numerous ..
  5. Uniform length DNA for paired end nextgen sequencing via in vitro packaging
    Frederick R Blattner; Fiscal Year: 2010
    ..The potential impact this technology will have on diagnosis, staging and treatment of human disease is immense. ..
  6. Phage-based targeted imaging probes and their application to vascular diseases
    Trevor Douglas; Fiscal Year: 2013
    ..Based on strong preliminary results, both in vitro and in vivo, our approach will combine the use of bacteriophage P22 capsids as biotemplates for construction of high performance multifunctional MR contrast agents...
  7. DNA packaging and delivery by dsDNA viruses
    Sherwood R Casjens; Fiscal Year: 2012
    ..and function focuses on the study of the proteins that are part of the portal vertex molecular machine of bacteriophage P22. The roles of these proteins, which form the motor that packages DNA and releases DNA during injection, are ..
  8. Multisubunit viral ATPases that couple ATP-hydrolysis to genome translocation
    Gino Cingolani; Fiscal Year: 2013
    ..will use a combination of crystallographic and biochemical techniques to characterize the structure of the bacteriophage P22 genome-packaging motor...
  9. Multisubunit viral ATPases that couple ATP-hydrolysis to genome translocation
    Gino Cingolani; Fiscal Year: 2011
    ..will use a combination of crystallographic and biochemical techniques to characterize the structure of the bacteriophage P22 genome-translocating motor...
  10. Understanding the Protein:Protein Interactions Required for Virus Assembly
    Carolyn M Teschke; Fiscal Year: 2013
    ..Capsid assembly will be investigated using bacteriophage P22 as a model dsDNA virus...
  11. Acquisition of a 200 kV Cryo-Electron Microscope
    David Chaplin; Fiscal Year: 2005
    ..These include determination of the mechanisms underlying maturation of the bacteriophage P22 (P. Prevelige), analysis of interactions between an RNA viral genome and the viral capsid (A...
  12. VIRAL REGULATORY MECHANISMS IN INFECTION AND LYSOGENY
    Ethel Jackson; Fiscal Year: 1980
    ..understanding the regulatory mechanisms which govern the life cycle of Salmonella typhimurium temperate bacteriophage P22. These studies approach two central questions...
  13. STRUCTURAL BASIS OF GROEL/SUBSTRATE INTERACTIONS
    JOHN ROSENBERG; Fiscal Year: 2000
    ..Additional goals would be to cocrystallize GroEL with additional substrate proteins including the bacteriophage P22 capsid, mouse aldehyde dehydrogenase and proteolytic fragments of the HK97 capsid...
  14. STRUCTURAL BASIS OF BACTERIOPHAGE P22 ASSEMBLY
    Wah Chiu; Fiscal Year: 2002
    ..b>Bacteriophage P22 is among the most comprehensively studied bacteriophages using biochemical and genetic tools...
  15. FOLDING OF PHAGE P22 STRUCTURAL PROTEINS
    CAROLYN TESCHKE; Fiscal Year: 1999
    ..Coat protein from the Salmonella bacteriophage P22 provides a unique model system with which to study the features of in vivo folding intermediates that are ..
  16. SUBUNIT INTERACTIONS DURING ICOSAHEDRAL CAPSID ASSEMBLY
    Peter Prevelige; Fiscal Year: 2007
    ..Therefore, we will study the structural transformation accompanying DNA packaging in bacteriophage P22 and the portal or connector motor protein complex function in bacteriophage Phi-29...
  17. BACTERIOPHAGE P22 ESSENTIAL RECOMBINATION FUNCTION
    ANTHONY POTEETE; Fiscal Year: 1993
    ..The system to be studied is the homologous recombination system of bacteriophage P22, which consists of the protein products of four phage genes: Erf, the key component, a strand-exchanging ..
  18. ENERGETIC ANALYSIS OF PROTEIN/DNA RECOGNITION
    Bruce Tidor; Fiscal Year: 2001
    ..two transcriptional regulatory proteins, the CI repressor from bacteriophage 434 and the Arc repressor from bacteriophage P22. The long-term objective of this work is to understand the relationship between the structure of these ..
  19. Cryoprobe Accessory for a 600 MHz NMR Spectrometer
    NEPALLI KRISHNA; Fiscal Year: 2006
    ..A-l, interferon-tau, cardiac muscle regulatory proteins, interaction of calmodulin with HIV gp41, bacteriophage P22 scaffolding protein, drug/DNA complexes, surface oligosaccahrides isolated from the Bacillus anthracis ..
  20. Plant Viruses as Platforms for Biomaterials
    John Johnson; Fiscal Year: 2006
    ....
  21. HIGH RESOLUTION OF STRUCTURAL STUDIES OF INSECT VIRUSES
    John Johnson; Fiscal Year: 2001
    ....
  22. FASEB CONFERENCE: VIRUS ASSEMBLY
    John Johnson; Fiscal Year: 2002
    ..We also seek to encourage unconventional aspects of virology as represented at our last meeting by presentations describing plant viruses as bio materials and nano reaction vessels. ..
  23. STRUCTURES, INTERACTIONS AND DYNAMICS OF NUCLEIC ACIDS
    George Thomas; Fiscal Year: 2006
    ..These studies will build upon the foundation established during prior support periods and are expected to yield new insights into biologically important DNA structural phenomena. [unreadable] [unreadable]..
  24. FOLDING OF PHAGE P22 STRUCTURAL PROTEINS
    CAROLYN TESCHKE; Fiscal Year: 2003
    ..The range of approaches will allow insight into the factors which control protein folding and assembly in vivo. This insight is important since misfolding and/or protein misassembly has been implicated in serious diseases. ..
  25. Biophysical studies of a Sulfolobus icosahedral virus
    Reza Khayat; Fiscal Year: 2007
    ..Detailed characterization of Archaea viruses may also expand our knowledge of viral evolution. ..
  26. Architecture of Viral DNA Delivery Machinery
    Gino Cingolani; Fiscal Year: 2008
    ..To achieve this ambitious task, we will study bacteriophage P22 DNA-delivery machinery...
  27. MOLECULAR STRUCTURE AND INTERACTIONS OF VIRUSES
    George Thomas; Fiscal Year: 2008
    ..The biological significance of the proposed research derives from the critical need for fundamental knowledge about the molecular basis of virus assembly in order to understand and control viral diseases. ..
  28. Function of SecA2 in Mycobacterium tuberculosis protein export
    CAROLYN TESCHKE; Fiscal Year: 2008
    ..In order to develop logical targets for new drugs, the physiology of Mycobacteria must be better understood. [unreadable] [unreadable] [unreadable]..