bacteriophage t7

Summary

Summary: Virulent bacteriophage and type species of the genus T7-like Phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.

Top Publications

  1. ncbi Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution
    S DOUBLIE
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 391:251-8. 1998
  2. ncbi Predicting evolution from genomics: experimental evolution of bacteriophage T7
    J J Bull
    Integrative Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, TX, USA
    Heredity (Edinb) 100:453-63. 2008
  3. pmc The phenotype-fitness map in experimental evolution of phages
    James J Bull
    The Institute for Cellular and Molecular Biology, Center for Computational Biology and Bioinformatics, Section of Integrative Biology, The University of Texas at Austin, Austin, Texas, United States of America
    PLoS ONE 6:e27796. 2011
  4. pmc Choreography of bacteriophage T7 DNA replication
    Seung Joo Lee
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Curr Opin Chem Biol 15:580-6. 2011
  5. pmc Slow fitness recovery in a codon-modified viral genome
    J J Bull
    The Institute for Cellular and Molecular Biology, The University of Texas at Austin
    Mol Biol Evol 29:2997-3004. 2012
  6. ncbi Inhibition of Escherichia coli RNA polymerase by bacteriophage T7 gene 2 protein
    S Nechaev
    Rutgers, The State University, Piscataway, NJ, 08854, USA
    J Mol Biol 289:815-26. 1999
  7. pmc Molecular mechanism of transcription inhibition by phage T7 gp2 protein
    Vladimir Mekler
    Department of Molecular Biology and Biochemistry, Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    J Mol Biol 413:1016-27. 2011
  8. pmc Testing optimality with experimental evolution: lysis time in a bacteriophage
    Richard H Heineman
    Section of Integrative Biology, University of Texas, Austin, Texas 78712, USA
    Evolution 61:1695-709. 2007
  9. pmc T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site
    Beatriz Cámara
    Department of Microbiology and Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, United Kingdom
    Proc Natl Acad Sci U S A 107:2247-52. 2010
  10. ncbi Structure of a T7 RNA polymerase elongation complex at 2.9 A resolution
    Tahir H Tahirov
    High Throughput Factory, RIKEN Harima Institute at SPring 8, 1 1 1 Kouto, Mikazuki cho, Sayo, Hyogo 679 5148, Japan
    Nature 420:43-50. 2002

Detail Information

Publications261 found, 100 shown here

  1. ncbi Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution
    S DOUBLIE
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 391:251-8. 1998
    ..Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a nucleoside triphosphate in the polymerase active site...
  2. ncbi Predicting evolution from genomics: experimental evolution of bacteriophage T7
    J J Bull
    Integrative Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, TX, USA
    Heredity (Edinb) 100:453-63. 2008
    ..of molecular biology has been exploited in designing and interpreting experimental evolution studies with bacteriophage T7. The modest size of its genome (40 kb dsDNA) and the ease of making genetic constructs, combined with the ..
  3. pmc The phenotype-fitness map in experimental evolution of phages
    James J Bull
    The Institute for Cellular and Molecular Biology, Center for Computational Biology and Bioinformatics, Section of Integrative Biology, The University of Texas at Austin, Austin, Texas, United States of America
    PLoS ONE 6:e27796. 2011
    ..In conjunction with data provided here, the new understanding appears to resolve a discrepancy between the reported fitness of phage T7 and the substantially lower value calculated from its phenotype-fitness map...
  4. pmc Choreography of bacteriophage T7 DNA replication
    Seung Joo Lee
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Curr Opin Chem Biol 15:580-6. 2011
    ..The lagging strand is synthesized discontinuously within a loop that forms and resolves during each cycle of Okazaki fragment synthesis. The synthesis of a primer as well as the termination of a fragment signal loop resolution...
  5. pmc Slow fitness recovery in a codon-modified viral genome
    J J Bull
    The Institute for Cellular and Molecular Biology, The University of Texas at Austin
    Mol Biol Evol 29:2997-3004. 2012
    ..Overall, the study supports the premise that codon-modified viruses recover fitness slowly, although the evolution is substantially more rapid than expected from the design principle...
  6. ncbi Inhibition of Escherichia coli RNA polymerase by bacteriophage T7 gene 2 protein
    S Nechaev
    Rutgers, The State University, Piscataway, NJ, 08854, USA
    J Mol Biol 289:815-26. 1999
    The 64 amino acid residue product of bacteriophage T7 gene 2 (gp2) binds the Escherichia coli RNA polymerase and inhibits transcription...
  7. pmc Molecular mechanism of transcription inhibition by phage T7 gp2 protein
    Vladimir Mekler
    Department of Molecular Biology and Biochemistry, Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    J Mol Biol 413:1016-27. 2011
    ..The inhibition of RNAP interactions with single-stranded segments of the transcription bubble by gp2 is a novel effect, which may occur via allosteric mechanism that is set in motion by the gp2 binding to the β' jaw...
  8. pmc Testing optimality with experimental evolution: lysis time in a bacteriophage
    Richard H Heineman
    Section of Integrative Biology, University of Texas, Austin, Texas 78712, USA
    Evolution 61:1695-709. 2007
    ..Here we evolved lysis time in bacteriophage T7, a virus of Escherichia coli...
  9. pmc T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site
    Beatriz Cámara
    Department of Microbiology and Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, United Kingdom
    Proc Natl Acad Sci U S A 107:2247-52. 2010
    ....
  10. ncbi Structure of a T7 RNA polymerase elongation complex at 2.9 A resolution
    Tahir H Tahirov
    High Throughput Factory, RIKEN Harima Institute at SPring 8, 1 1 1 Kouto, Mikazuki cho, Sayo, Hyogo 679 5148, Japan
    Nature 420:43-50. 2002
    The single-subunit bacteriophage T7 RNA polymerase carries out the transcription cycle in an identical manner to that of bacterial and eukaryotic multisubunit enzymes...
  11. pmc The role of the T7 Gp2 inhibitor of host RNA polymerase in phage development
    Dhruti Savalia
    Waksman Institute for Microbiology, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA
    J Mol Biol 402:118-26. 2010
    b>Bacteriophage T7 relies on its own RNA polymerase (RNAp) to transcribe its middle and late genes. Early genes, which include the viral RNAp gene, are transcribed by the host RNAp from three closely spaced strong promoters-A1, A2, and A3...
  12. pmc The bacteriophage t7 virion undergoes extensive structural remodeling during infection
    Bo Hu
    Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, TX 77030, USA
    Science 339:576-9. 2013
    ..After ejection, the extended phage tail collapsed or disassembled, which allowed resealing of the infected cell membrane. These structural studies provide a detailed series of intermediates during phage infection...
  13. ncbi T7 phage display of Ep15 peptide for the detection of WNV IgG
    Sebastien Herrmann
    Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Virol Methods 141:133-40. 2007
    ....
  14. ncbi Isolation and identification of fxsA, an Escherichia coli gene that can suppress F exclusion of bacteriophage T7
    W F Wang
    Department of Microbiology and Institute for Cell and Molecular Biology, University of Texas, Austin, TX 78712 1095, USA
    J Mol Biol 292:485-99. 1999
    A selection for mutants of Escherichia coli that survive coexpression of bacteriophage T7 gene 10 and plasmid F pifA has allowed the identification of a newly defined genetic locus, fxsA. fxsA is located at 94.1 min on the E...
  15. pmc Single-molecule studies reveal dynamics of DNA unwinding by the ring-shaped T7 helicase
    Daniel S Johnson
    Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, NY 14853, USA
    Cell 129:1299-309. 2007
    ..However, an active unwinding model fully supports the data even though the helicase on its own does not unwind at its optimal rate. This work offers insights into possible ways helicase activity is enhanced by associated proteins...
  16. ncbi Peptidoglycan hydrolytic activities associated with bacteriophage virions
    Michael Moak
    Molecular Genetics and Microbiology, and Institute for Cell and Molecular Biology, University of Texas, Austin, TX 78712 1095, USA
    Mol Microbiol 51:1169-83. 2004
    ..These studies lead to a general mechanism describing how phage genomes are transported across the bacterial cell wall...
  17. pmc Localization of the Escherichia coli RNA polymerase beta' subunit residue phosphorylated by bacteriophage T7 kinase Gp0.7
    Elena Severinova
    Waksman Institute, Piscataway, NJ 08854, USA
    J Bacteriol 188:3470-6. 2006
    During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7...
  18. pmc The arginine finger of bacteriophage T7 gene 4 helicase: role in energy coupling
    Donald J Crampton
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:4373-8. 2004
    The DNA helicase encoded by gene 4 of bacteriophage T7 couples DNA unwinding to the hydrolysis of dTTP...
  19. ncbi Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7
    Geok Hun Tan
    Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    J Med Virol 77:475-80. 2005
    ..The recombinant phage exhibited the antigenic and immunogenic characteristics of HBsAg, illustrating its potential as an immunological reagent and vaccine...
  20. ncbi Quasi-atomic model of bacteriophage t7 procapsid shell: insights into the structure and evolution of a basic fold
    Xabier Agirrezabala
    Department of Structure of Macromolecules, Centro Nacional de Biotecnologia, CSIC, C Darwin 3, Cantoblanco, 28049 Madrid, Spain
    Structure 15:461-72. 2007
    ..are emerging as one such family, and we have studied the possible existence of the HK97-like fold in bacteriophage T7. The procapsid structure at approximately 10 A resolution was used to obtain a quasi-atomic model by fitting ..
  21. ncbi Structure and function of hexameric helicases
    S S Patel
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Annu Rev Biochem 69:651-97. 2000
    ..binding was observed in many, and sequential NTPase catalysis has been observed in two proteins, gp4 of bacteriophage T7 and rho of Escherichia coli...
  22. ncbi Crystal structure of the Holliday junction resolving enzyme T7 endonuclease I
    J M Hadden
    Astbury Centre for Structural Molecular Biology, School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    Nat Struct Biol 8:62-7. 2001
    ..T7 endonuclease I therefore represents the first crystal structure of a junction resolving enzyme that is a member of the nuclease superfamily of enzymes...
  23. pmc Substitutions in the Escherichia coli RNA polymerase inhibitor T7 Gp2 that allow inhibition of transcription when the primary interaction interface between Gp2 and RNA polymerase becomes compromised
    Andrey Shadrin
    Section of Microbiology and MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, UK
    Microbiology 158:2753-64. 2012
    The Escherichia coli-infecting bacteriophage T7 encodes a 7 kDa protein, called Gp2, which is a potent inhibitor of the host RNA polymerase (RNAp). Gp2 is essential for T7 phage development. The interaction site for Gp2 on the E...
  24. pmc Bypass of a nick by the replisome of bacteriophage T7
    Bin Zhu
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 286:28488-97. 2011
    ..In bacteriophage T7, movement of either the DNA helicase or the DNA polymerase alone terminates upon encountering a nick in ..
  25. pmc Molecular rearrangements involved in the capsid shell maturation of bacteriophage T7
    Alina Ionel
    Department of Macromolecular Structure, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Darwin 3, Cantoblanco, 28049 Madrid, Spain
    J Biol Chem 286:234-42. 2011
    ....
  26. ncbi A novel nucleotide kinase encoded by gene 1.7 of bacteriophage T7
    Ngoc Q Tran
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 77:492-504. 2010
    Gene 1.7 of bacteriophage T7 confers sensitivity of both phage T7 and its host Escherichia coli to dideoxythymidine (ddT). We have purified the product of gene 1.7, gp1.7. It exists in two forms of molecular weight 22,181 and 17,782...
  27. ncbi The Trp cage motif as a scaffold for the display of a randomized peptide library on bacteriophage T7
    Richard E Herman
    Nastech Pharmaceutical Company, Inc, Bothell, Washington 98021, USA
    J Biol Chem 282:9813-24. 2007
    ....
  28. ncbi Effects of anticancer drugs on transcription in vitro
    D Wilmanska
    Department of General Chemistry, Institute of Physiology and Biochemistry, Medical University of Lodz, Poland
    Z Naturforsch C 56:886-91. 2001
    ..The results indicate that the effects primarily depend on sequence specificity and secondarily on the dissociation rate of ligands from their complexes with DNA...
  29. ncbi Amino acid changes in a unique sequence of bacteriophage T7 DNA polymerase alter the processivity of nucleotide polymerization
    X M Yang
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 272:6599-606. 1997
    ..The observed dissociation constant of the gene 5 protein K(300,302,304)E-thioredoxin complex is 7-fold higher than that of the wild type complex. The altered polymerase also has a lower binding affinity for double-stranded DNA...
  30. ncbi Inadequate inhibition of host RNA polymerase restricts T7 bacteriophage growth on hosts overexpressing udk
    Udi Qimron
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    Mol Microbiol 67:448-57. 2008
    ..5 that affect its interaction with T7 RNA polymerase, also reduce the interference with T7 growth by host RNA polymerase. We propose a general model for the requirement of host RNA polymerase inhibition...
  31. ncbi Analysis of the effect of bulk at N2-alkylguanine DNA adducts on catalytic efficiency and fidelity of the processive DNA polymerases bacteriophage T7 exonuclease- and HIV-1 reverse transcriptase
    Jeong Yun Choi
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 279:19217-29. 2004
    ..with the processive DNA polymerases human immunodeficiency virus, type 1, reverse transcriptase (RT), and bacteriophage T7 exonuclease(-) (T7(-))...
  32. pmc Structure of the receptor-binding carboxy-terminal domain of bacteriophage T7 tail fibers
    Carmela Garcia-Doval
    Department of Molecular Structure, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, E 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 109:9390-5. 2012
    The six bacteriophage T7 tail fibers, homo-trimers of gene product 17, are thought to be responsible for the first specific, albeit reversible, attachment to Escherichia coli lipopolysaccharide...
  33. ncbi The linker region between the helicase and primase domains of the gene 4 protein of bacteriophage T7. Role in helicase conformation and activity
    Seung Joo Lee
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:23384-93. 2004
    The gene 4 protein of bacteriophage T7 provides both helicase and primase activities...
  34. pmc Gp15 and gp16 cooperate in translocating bacteriophage T7 DNA into the infected cell
    Chung Yu Chang
    Section of Molecular Genetics and Microbiology, and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Virology 398:176-86. 2010
    Loss of up to four amino acids from the C terminus of the 1318 residue bacteriophage T7 gp16 allows plaque formation at normal efficiencies...
  35. ncbi Specific single-stranded breaks in mature bacteriophage T7 DNA
    S A Khan
    Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284 7760, USA
    Virology 211:329-31. 1995
    ..We propose that the T7 nicks are produced by premature activity of the T7 terminase during DNA packaging...
  36. pmc The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase
    X Cheng
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, NY 11724
    Proc Natl Acad Sci U S A 91:4034-8. 1994
    The lysozyme of bacteriophage T7 is a bifunctional protein that cuts amide bonds in the bacterial cell wall and binds to and inhibits transcription by T7 RNA polymerase...
  37. pmc C-terminal phenylalanine of bacteriophage T7 single-stranded DNA-binding protein is essential for strand displacement synthesis by T7 DNA polymerase at a nick in DNA
    Sharmistha Ghosh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 284:30339-49. 2009
    Single-stranded DNA-binding protein (gp2.5), encoded by gene 2.5 of bacteriophage T7, plays an essential role in DNA replication...
  38. pmc Escherichia coli K1's capsule is a barrier to bacteriophage T7
    Dean Scholl
    The National Institute of Mental Health, National Institutes of Health, Building 36, Room 3D20, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Appl Environ Microbiol 71:4872-4. 2005
    ..This observation suggests that the capsule plays an important role as a defense against some phages that recognize structures beneath it and that the K1-specific phages evolved to counter this physical barrier...
  39. pmc Evolution at a high imposed mutation rate: adaptation obscures the load in phage T7
    R Springman
    Institute for Cellular and Molecular Biology, Center for Computational Biology and Bioinformatics, Section of Integrative Biology, Section of Molecular Genetics and Microbiology, University of Texas, Austin, Texas 78712, USA
    Genetics 184:221-32. 2010
    ..The impact of a high mutation rate on long-term viral fitness was tested here. A large population of the DNA bacteriophage T7 was grown with a mutagen, producing a genomic rate of 4 nonlethal mutations per generation, two to three ..
  40. ncbi Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7
    Ying Li
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 11:784-90. 2004
    ..the structural basis for this, we determined crystal structures of a replicative DNA polymerase from bacteriophage T7 in complex with nucleotide substrates and a DNA template containing a cis-syn cyclobutane pyrimidine dimer (..
  41. ncbi Molecular mechanisms in bacteriophage T7 procapsid assembly, maturation, and DNA containment
    Mario E Cerritelli
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Adv Protein Chem 64:301-23. 2003
    b>Bacteriophage T7 is a double-stranded DNA bacteriophage that has attracted particular interest in studies of gene expression and regulation and of morphogenesis, as well as in biotechnological applications of expression vectors and phage ..
  42. ncbi Inhibition of bacteriophage T7 RNA polymerase in vitro transcription by DNA-binding pyrrolo[2,1-c][1,4]benzodiazepines
    M S Puvvada
    Gene Targeted Drug Design Research Group, School of Pharmacy and Biomedical Science, University of Portsmouth, U K
    Biochemistry 36:2478-84. 1997
    ....
  43. ncbi Changing the enzymatic activity of T7 endonuclease by mutations at the beta-bridge site: alteration of substrate specificity profile and metal ion requirements by mutation distant from the catalytic domain
    Chudi Guan
    New England Biolabs, Inc, 32 Tozer Rd, Beverly, Massachusetts 01915, USA
    Biochemistry 43:4313-22. 2004
    ..Our results suggest that the enzyme recognizes its substrates by DNA conformation exclusion and offer a simple explanation for the broad substrate specificity of phage resolvase...
  44. pmc Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage
    Udi Qimron
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:19039-44. 2006
    ..coli lipopolysaccharide receptor for T7 adsorption leads to T7 resistance. Selection of T7 phage that can recognize these altered receptors has enabled the construction of phage to which the host is 100-fold less resistant...
  45. pmc DNA synthesis provides the driving force to accelerate DNA unwinding by a helicase
    Natalie M Stano
    Department of Biochemistry, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Nature 435:370-3. 2005
    ..The ring-shaped helicase of bacteriophage T7 translocates along single-stranded (ss)DNA at a speed of 130 bases per second; however, T7 helicase slows ..
  46. ncbi Complete nucleotide sequence and likely recombinatorial origin of bacteriophage T3
    Maria I Pajunen
    Department of Medical Biochemistry and Molecular Biology, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, Finland
    J Mol Biol 319:1115-32. 2002
    ..This represents the first experimentally observed recombination between lytic phages whose normal hosts are different bacterial genera...
  47. ncbi Binding of the priming nucleotide in the initiation of transcription by T7 RNA polymerase
    Iaroslav Kuzmine
    Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Biol Chem 278:2819-23. 2003
    ....
  48. ncbi Role of T7 RNA polymerase His784 in start site selection and initial transcription
    Luis G Brieba
    Department of Biochemistry, University of Texas Health Sciences Center, 7703 Floyd Curl Drive, San Antonio, Texas 78284 7760, USA
    Biochemistry 41:5144-9. 2002
    ..This interaction is also important for holding short transcripts within the transcription complex during initial transcription...
  49. ncbi Essential lysine residues in the RNA polymerase domain of the gene 4 primase-helicase of bacteriophage T7
    S J Lee
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:49419-26. 2001
    ..In the bacteriophage T7 replication system, DNA primase is encoded by gene 4 of the phage...
  50. pmc Interactions of Escherichia coli thioredoxin, the processivity factor, with bacteriophage T7 DNA polymerase and helicase
    Sharmistha Ghosh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:32077-84. 2008
    ..of 71 amino acid residues, designated the thioredoxin binding domain (TBD), located in the thumb subdomain of bacteriophage T7 gene 5 DNA polymerase. The initial designation of thioredoxin as a processivity factor was premature...
  51. ncbi Autoantibodies as potential biomarkers for nasopharyngeal carcinoma
    Yong qing Tong
    Cancer Research Institute, Xiang Ya School of Medicine, Central South University, Hunan, PR China
    Proteomics 8:3185-93. 2008
    ..Studies of the corresponding proteins may have significances in tumor biology, novel drug development, and immunotherapy...
  52. ncbi Genomic analysis of bacteriophages SP6 and K1-5, an estranged subgroup of the T7 supergroup
    D Scholl
    Section of Biochemical Genetics, The National Institute of Mental Health, NIH, 9000 Rockville Pike, Bethesda, MD 20895, USA
    J Mol Biol 335:1151-71. 2004
    ....
  53. pmc The complex between a four-way DNA junction and T7 endonuclease I
    Anne Cécile Déclais
    Cancer Research UK Nucleic Acid Structure Research Group, Department of Biochemistry, MSI WTB complex, The University of Dundee, Dundee DD1 5EH, UK
    EMBO J 22:1398-409. 2003
    ..This shows how the enzyme is selective for the structure of a four-way junction, such that both continuous strands can be accommodated into the two active sites so that a productive resolution event is possible...
  54. ncbi Experimental evolution yields hundreds of mutations in a functional viral genome
    J J Bull
    Section of Integrated Biology, University of Texas at Austin, Austin, TX 78712, USA
    J Mol Evol 57:241-8. 2003
    Two lines of the bacteriophage T7 were grown to fix mutations indiscriminately, using a combination of population bottlenecks and mutagenesis...
  55. ncbi A system for the directed evolution of the insecticidal protein from Bacillus thuringiensis
    Hiroshi Ishikawa
    Graduate School of Bio Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Naka cho 2 24 16, Koganei, Tokyo 184 8588, Japan
    Mol Biotechnol 36:90-101. 2007
    ..These results indicate that a method combining T7 phage display with selection using cadherin-like protein-coated magnetic beads can be used to increase the activity of easily obtained, low-activity Cry toxins from bacteria...
  56. ncbi A positive charge at position 33 of thioredoxin primarily affects its interaction with other proteins but not redox potential
    Tiao Yin Lin
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China
    Biochemistry 43:945-52. 2004
    ..Together with the G33D mutation, the overall results show that a charged residue at the first X has a greater influence on the molecular interaction of the protein than the redox potential...
  57. pmc Promiscuous usage of nucleotides by the DNA helicase of bacteriophage T7: determinants of nucleotide specificity
    Ajit K Satapathy
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 284:14286-95. 2009
    The multifunctional protein encoded by gene 4 of bacteriophage T7 (gp4) provides both helicase and primase activity at the replication fork...
  58. ncbi T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries
    Lauren R H Krumpe
    Basic Research Program, SAIC Frederick, Inc, Frederick, MD, USA
    Proteomics 6:4210-22. 2006
    ..To demonstrate their utility, several of the T7-displayed peptide libraries were screened for streptavidin- and neutravidin-binding phage. Novel binding motifs were identified for each protein...
  59. ncbi Changes in bacteriophage T7 virion structure at the initiation of infection
    Priscilla Kemp
    Molecular Genetics and Microbiology, University of Texas, Austin, 78712, USA
    Virology 340:307-17. 2005
    Five proteins are ejected from the bacteriophage T7 virion at the initiation of infection...
  60. ncbi A general mechanism for viral resistance to suicide gene expression
    J J Bull
    Section of Integrative Biology, University of Texas, Austin 78712 1023, USA
    J Mol Evol 53:47-54. 2001
    b>Bacteriophage T7 was challenged with either of two toxic genes expressed from plasmids. Each plasmid contained a different gene downstream of a T7 promoter; cells harboring each plasmid caused an infection by wild-type T7 to abort...
  61. ncbi The crystal structure of the bifunctional primase-helicase of bacteriophage T7
    Eric A Toth
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell 12:1113-23. 2003
    Within minutes after infecting Escherichia coli, bacteriophage T7 synthesizes many copies of its genomic DNA...
  62. pmc Effects of Escherichia coli physiology on growth of phage T7 in vivo and in silico
    Lingchong You
    Department of Chemical Engineering, University of Wisconsin Madison, Madison, Wisconsin 53706 1691, USA
    J Bacteriol 184:1888-94. 2002
    ..Finally, the simulation was used to follow how bottlenecks to phage growth shift in response to variations in host or phage functions...
  63. ncbi Expression and purification of histidine-tagged bacteriophage T7 DNA polymerase
    Marina Schlicke
    Applied Molecular Evolution, Institute for Biology II, University of Leipzig, Liebigstrasse 18, D 04103 Leipzig, Germany
    Protein Expr Purif 39:247-53. 2005
    ..This phenomenon is also observed with bacteriophage T7 gene 5 protein, the phage-encoded subunit of T7 DNA polymerase, if expression is based on the T5 promoter/lac ..
  64. pmc A unique loop in T7 DNA polymerase mediates the binding of helicase-primase, DNA binding protein, and processivity factor
    Samir M Hamdan
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:5096-101. 2005
    b>Bacteriophage T7 DNA polymerase (gene 5 protein, gp5) interacts with its processivity factor, Escherichia coli thioredoxin, via a unique loop at the tip of the thumb subdomain...
  65. ncbi Acidic residues in the nucleotide-binding site of the bacteriophage T7 DNA primase
    Seung Joo Lee
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:26984-91. 2005
    ..Like other prokaryotic homologs, the primase domain of the gene 4 helicase-primase of bacteriophage T7 contains a zinc motif and a catalytic core...
  66. ncbi Lagging strand synthesis in coordinated DNA synthesis by bacteriophage t7 replication proteins
    JoonSoo Lee
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA
    J Mol Biol 316:19-34. 2002
    The proteins of bacteriophage T7 DNA replication mediate coordinated leading and lagging strand synthesis on a minicircle template...
  67. ncbi Structure of the connector of bacteriophage T7 at 8A resolution: structural homologies of a basic component of a DNA translocating machinery
    Xabier Agirrezabala
    Centro Nacional de Biotecnologia, CSIC, Campus de la Universidad Autonoma de Madrid, Cantoblanco 28049 Madrid, Spain
    J Mol Biol 347:895-902. 2005
    The three-dimensional structure of the bacteriophage T7 head-to-tail connector has been obtained at 8A resolution using cryo-electron microscopy and single-particle analysis from purified recombinant connectors...
  68. ncbi Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA
    M D Walkinshaw
    Institute of Cell and Molecular Biology, The King s Buildings, University of Edinburgh, EH9 3JR, Edinburgh, United Kingdom
    Mol Cell 9:187-94. 2002
    ..This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of negative charges along the phosphate ..
  69. ncbi The DNA binding domain of the gene 2.5 single-stranded DNA-binding protein of bacteriophage T7
    Edel M Hyland
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:7247-56. 2003
    Gene 2.5 of bacteriophage T7 encodes a single-stranded DNA-binding protein that is essential for viral survival...
  70. pmc Distinguishing between selection and population expansion in an experimental lineage of bacteriophage T7
    Matthew W Hahn
    Evolution, Ecology, and Organismal Biology Group, Department of Biology, Duke University, Durham, North Carolina 27708, USA
    Genetics 161:11-20. 2002
    ..Here, we characterize DNA polymorphism data over time for four genes in bacteriophage T7. Our experiment ran for 2500 generations and populations were sampled after 500, 2000, and 2500 generations...
  71. ncbi A second symmetry mismatch at the portal vertex of bacteriophage T7: 8-fold symmetry in the procapsid core
    Mario E Cerritelli
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, MSC 8025, Bethesda, MD 20892 8025, USA
    J Mol Biol 327:1-6. 2003
    ..We have investigated the core structure by cryo-electron microscopy and image analysis of procapsids and find that it observes 8-fold symmetry. Stoichiometry data indicate that its major constituent is an octamer of gp15...
  72. ncbi Effect of single-stranded DNA-binding proteins on the helicase and primase activities of the bacteriophage T7 gene 4 protein
    Zheng Guo He
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:22190-7. 2004
    Gene 4 protein (gp4) of bacteriophage T7 provides two essential functions at the T7 replication fork, primase and helicase activities. Previous studies have shown that the single-stranded DNA-binding protein of T7, encoded by gene 2...
  73. ncbi Essential amino acid residues in the single-stranded DNA-binding protein of bacteriophage T7. Identification of the dimer interface
    Lisa F Rezende
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:50643-53. 2002
    Gene 2.5 of bacteriophage T7 is an essential gene that encodes a single-stranded DNA-binding protein. T7 phage with gene 2.5 deleted can grow only on Escherichia coli cells that express gene 2.5 from a plasmid...
  74. ncbi Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase
    Y Whitney Yin
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520 8114, USA
    Science 298:1387-95. 2002
    To make messenger RNA transcripts, bacteriophage T7 RNA polymerase (T7 RNAP) undergoes a transition from an initiation phase, which only makes short RNA fragments, to a stable elongation phase. We have determined at 2...
  75. ncbi T7 DNA helicase: a molecular motor that processively and unidirectionally translocates along single-stranded DNA
    Dong Eun Kim
    Department of Biochemistry, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Mol Biol 321:807-19. 2002
    ..Here, we measure the translocation and energy coupling efficiency of a replicative DNA helicase from bacteriophage T7 that is a member of a class of helicases that assembles into ring-shaped hexamers...
  76. pmc The DNA-unwinding mechanism of the ring helicase of bacteriophage T7
    Yong Joo Jeong
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 101:7264-9. 2004
    ..b>Bacteriophage T7 helicase functions as a hexameric ring to drive the replication complex by separating the DNA strands during ..
  77. ncbi A single-stranded DNA-binding protein of bacteriophage T7 defective in DNA annealing
    Lisa F Rezende
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:29098-105. 2003
    ..In bacteriophage T7-infected cells, the product of viral gene 2.5, a single-stranded DNA-binding protein, performs this function...
  78. ncbi Multiple roles of T7 RNA polymerase and T7 lysozyme during bacteriophage T7 infection
    Xing Zhang
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    J Mol Biol 340:707-30. 2004
    ....
  79. ncbi Modular architecture of the bacteriophage T7 primase couples RNA primer synthesis to DNA synthesis
    Masato Kato
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 11:1349-60. 2003
    ..We report a crystal structure of bacteriophage T7 primase that reveals its two domains and the presence of two Mg(2+) ions bound to the active site...
  80. ncbi The complete sequence of marine bacteriophage VpV262 infecting vibrio parahaemolyticus indicates that an ancestral component of a T7 viral supergroup is widespread in the marine environment
    Stephen C Hardies
    Department of Biochemistry, MSC 7760, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr, San Antonio, TX 78229 3900, USA
    Virology 310:359-71. 2003
    ..In the early and replicative segments, these phages have participated in extensive interchange with the viral gene pool. VpV262 carries a different replicative module than SIO1 and the T7-like phages...
  81. ncbi T7 lysozyme represses T7 RNA polymerase transcription by destabilizing the open complex during initiation
    Natalie M Stano
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 279:16136-43. 2004
    b>Bacteriophage T7 lysozyme binds to T7 RNA polymerase and inhibits transcription initiation and the transition from initiation to elongation...
  82. ncbi Bacteriophage T7 DNA ejection into cells is initiated by an enzyme-like mechanism
    Priscilla Kemp
    Section of Molecular Genetics and Microbiology, and Institute for Cell and Molecular Biology, University of Texas, Austin, TX 78712 1095, USA
    Mol Microbiol 53:1251-65. 2004
    In a normal infection about 850 bp of the bacteriophage T7 genome is ejected into the cell, the remainder of the genome is internalized through transcription by Escherichia coli and then T7 RNA polymerase...
  83. ncbi Experimental genomic evolution: extensive compensation for loss of DNA ligase activity in a virus
    D Rokyta
    Section of Integrative Biology, University of Texas, Austin, TX 78712, USA
    Mol Biol Evol 19:230-8. 2002
    Deletion of the viral ligase gene drastically reduced the fitness of bacteriophage T7 on a ligase-deficient host...
  84. pmc T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries
    S Danner
    Skirball Institute of Biomolecular Medicine and Department of Microbiology, New York University School of Medicine, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 98:12954-9. 2001
    ....
  85. pmc An in trans interaction at the interface of the helicase and primase domains of the hexameric gene 4 protein of bacteriophage T7 modulates their activities
    Bin Zhu
    Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 284:23842-51. 2009
    ..The two activities reside in a single polypeptide encoded by gene 4 of bacteriophage T7. Their coexistence within the same polypeptide facilitates their coordination during DNA replication...
  86. pmc Computation, prediction, and experimental tests of fitness for bacteriophage T7 mutants with permuted genomes
    D Endy
    The Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, CA 94704, USA
    Proc Natl Acad Sci U S A 97:5375-80. 2000
    We created a simulation based on experimental data from bacteriophage T7 that computes the developmental cycle of the wild-type phage and also of mutants that have an altered genome order...
  87. ncbi Oligomeric states of bacteriophage T7 gene 4 primase/helicase
    Donald J Crampton
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    J Mol Biol 360:667-77. 2006
    Electron microscopic and crystallographic data have shown that the gene 4 primase/helicase encoded by bacteriophage T7 can form both hexamers and heptamers...
  88. ncbi Stopped-flow kinetic analysis of the interaction of Escherichia coli RNA polymerase with the bacteriophage T7 A1 promoter
    R S Johnson
    Department of Biochemistry, East Carolina University School of Medicine, Greenville, NC, 27858, USA
    J Mol Biol 283:353-70. 1998
    ..analysis of open complex formation between Escherichia coli RNA polymerase and the A1 promoter from bacteriophage T7 by monitoring alterations in the intrinsic protein fluorescence of RNA polymerase in stopped-flow kinetic ..
  89. pmc Hexagonally packed DNA within bacteriophage T7 stabilized by curvature stress
    T Odijk
    Faculty of Chemical Engineering and Materials Science, Delft University of Technology, The Netherlands
    Biophys J 75:1223-7. 1998
    A continuum computation is proposed for the bending stress stabilizing DNA that is hexagonally packed within bacteriophage T7. Because the inner radius of the DNA spool is rather small, the stress of the curved DNA genome is strong enough ..
  90. ncbi The C-terminal residues of bacteriophage T7 gene 4 helicase-primase coordinate helicase and DNA polymerase activities
    Seung Joo Lee
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:25841-9. 2006
    The gene 4 protein of bacteriophage T7 plays a central role in DNA replication by providing both helicase and primase activities...
  91. ncbi Evolutionary design on a budget: robustness and optimality of bacteriophage T7
    L You
    Department of Biomedical Engineering and Institute for Genome Sciences and Policy, Duke University, Durham, NC 27708, USA
    Syst Biol (Stevenage) 153:46-52. 2006
    ..In particular, the strong correlation between fitness and growth efficiency suggests that T7 may have evolved to maximise its growth rate by minimising waste of finite resources...
  92. pmc Exchange of DNA polymerases at the replication fork of bacteriophage T7
    Donald E Johnson
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:5312-7. 2007
    ....
  93. ncbi Nucleic acid unwinding by hepatitis C virus and bacteriophage t7 helicases is sensitive to base pair stability
    Ilker Donmez
    Department of Biochemistry, University of Medicine and Dentistry, New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 282:21116-23. 2007
    ..Modeling of the dependence provided the degree of the active involvement of helicase in base pair destabilization during the unwinding process and distinguished between passive and active mechanisms of unwinding...
  94. ncbi Bi-functional activities of chimeric lysozymes constructed by domain swapping between bacteriophage T7 and K11 lysozymes
    Ethel H Alcantara
    Research Center for Bio Medicinal Resources and Department of Life Science and Technology, Pai Chai University, 439 6 Doma dong, Seo Gu, Daejeon 302 735, Korea
    J Biochem Mol Biol 40:539-46. 2007
    The lysozymes encoded by bacteriophage T7 and K11 are both bifunctional enzymes sharing an extensive sequence homology (75%)...
  95. ncbi Coupling of rRNA transcription and ribosomal assembly in vivo. Formation of active ribosomal subunits in Escherichia coli requires transcription of rRNA genes by host RNA polymerase which cannot be replaced by bacteriophage T7 RNA polymerase
    B T Lewicki
    Max Planck Institut fur Molekulare Genetik, Abteilung Wittmann, Berlin, Germany
    J Mol Biol 231:581-93. 1993
    ..T7 RNA polymerase cannot replace the host RNA polymerase in this process at 37 degrees C...
  96. pmc Real-time single-molecule observation of rolling-circle DNA replication
    Nathan A Tanner
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Nucleic Acids Res 37:e27. 2009
    ..This method allows for rapid and precise characterization of the kinetics of DNA synthesis and the effects of replication inhibitors...
  97. pmc Transcription processing at 1,N2-ethenoguanine by human RNA polymerase II and bacteriophage T7 RNA polymerase
    Alexandra Dimitri
    Department of Biology, New York University, New York, NY 10003, USA
    J Mol Biol 375:353-66. 2008
    ..Our results show that 1,N(2)-epsilon G acts as a partial block to the bacteriophage T7 RNA polymerase (RNAP), which allows nucleotide incorporation in the growing RNA with the selectivity A>G&..
  98. ncbi A mutational analysis of DNA mimicry by ocr, the gene 0.3 antirestriction protein of bacteriophage T7
    Augoustinos S Stephanou
    EaStCHEM School of Chemistry, The University of Edinburgh, The King s Buildings, Edinburgh EH9 3JJ, UK
    Biochem Biophys Res Commun 378:129-32. 2009
    The ocr protein of bacteriophage T7 is a structural and electrostatic mimic of approximately 24 base pairs of double-stranded B-form DNA...
  99. pmc DNA recognition by the DNA primase of bacteriophage T7: a structure-function study of the zinc-binding domain
    Barak Akabayov
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biochemistry 48:1763-73. 2009
    Synthesis of oligoribonucleotide primers for lagging-strand DNA synthesis in the DNA replication system of bacteriophage T7 is catalyzed by the primase domain of the gene 4 helicase-primase...
  100. ncbi Motors, switches, and contacts in the replisome
    Samir M Hamdan
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Annu Rev Biochem 78:205-43. 2009
    ..The replisome of bacteriophage T7 contains a minimum of proteins, thus facilitating its study...
  101. ncbi DNA primase acts as a molecular brake in DNA replication
    Jong Bong Lee
    Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, 250 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 439:621-4. 2006
    ..Here we use single-molecule techniques to study the kinetics of a multiprotein replication complex from bacteriophage T7 and to characterize the effect of primase activity on fork progression...

Research Grants62

  1. Pyrophosphorolysis-activatable helicase dependent amplification assay for lung ca
    BERTRAND LEMIEUX; Fiscal Year: 2007
    ..In addition, some native enzymes (like the bacteriophage T7 DNA polymerase) have reduced specificity for deoxynucleotide triphosphates (dNTP) and may thus be ideal for ..
  2. Mechanisms of transcription initiation in mitochondria
    Dmitry Temiakov; Fiscal Year: 2013
    ..single-subunit RNAPs that is distinct from the multi-subunit cellular RNAPs but distantly related to RNAPs of bacteriophage T7, the pol I familty of DNA polymerases, and single-subunit RNAPs from chloroplasts...
  3. Polymerase Interactions with Carcinogen-modified DNA
    F Peter Guengerich; Fiscal Year: 2013
    ..polymerases will be done using rapid fluorescence kinetics (Dpo4 tryptophan mutants and a coumarin-labeled bacteriophage T7 polymerase mutant) and H-D exchange kinetics (Dpo4), with a view to events in incorporation opposite DNA ..
  4. Engineering Bacteriophage T7 RNA Polymerase/Promoter System for Mammalian Synthet
    NANETTE REGINA BOYLE; Fiscal Year: 2013
    ..The bacteriophage T7 RNA polymerase/promoter system is an excellent starting point for this project research due to its rapid ..
  5. MECHANISTIC STUDIES OF HEXAMERIC HELICASES
    Smita S Patel; Fiscal Year: 2013
    ..We propose studies of replicative enzymes of the bacteriophage T7 and the human mitochondria...
  6. Analysis of Protein-Ligand Binding on the Proteomic Scale
    Michael C Fitzgerald; Fiscal Year: 2012
    ..coli) bacteriophage T7 proteome, to analysis of protein-protein interactions in a subset of 11 proteins involved in a yeast cell-..
  7. Primer Synthesis and Handoff by Bacteriophage T7 DNA Primase
    ALFREDO JOSE HERNANDEZ; Fiscal Year: 2013
    ..are conserved among all biological entities, the relatively simplicity of the replication machinery of bacteriophage T7 makes it an ideal model system to study mechanistic aspects of DNA replication...
  8. Initiation and Elongation in T7 RNA Polymerase
    Craig T Martin; Fiscal Year: 2012
    ..and eukaryotic RNA polymerases, and exciting new structures for the single subunit phage polymerase from bacteriophage T7. The latter presents an ideal model system for the study of fundamental issues in transcription...
  9. Dynamics, Control, and Design of Bacteriophage T7
    Drew Endy; Fiscal Year: 2010
    ..progress on these questions we plan to work with a relatively well-characterized natural biological system, bacteriophage T7. Specifically, we propose to: (1) Design and construct a synthetic bacteriophage T7 genome that is based on ..
  10. Molecular tools for Bunyavirus antiviral screening
    PAUL OLIVO; Fiscal Year: 2005
    ..The screening tools will be based on virus promoter-driven reporter gene constructs generated by bacteriophage T7 RNA polymerase or mammalian RNA polymerase I...
  11. CYTOCHROME P-450XVII GENE STRUCTURE AND REGULATION
    H Fevold; Fiscal Year: 1990
    ..DNA sequencing will be performed by the dideoxy chain termination technique of Sanger, as modified to use the bacteriophage T7 DNA polymerase...
  12. Transcription coupled DNA repair in E. Coli
    Philip Hanawalt; Fiscal Year: 2006
    ..Therefore, we will focus upon the monomeric RNAP of bacteriophage T7 and the multisubunit RNAP of Escherichia coli. I...
  13. Identification of Novel Genes Expressed in Bowel Cancer Using Change Mediated Ant
    Jeffrey Hillman; Fiscal Year: 2007
    ..of human genomic DNA and cDNA from colorectal cancer tissue samples of subjects will be constructed in bacteriophage T7. Sufficient independent clones will be obtained to assure complete coverage of these libraries...
  14. ANTISENSE RNA INHIBITION OF HIV VIA RETROVIRAL VECTORS
    Eli Gilboa; Fiscal Year: 1991
    ..types of promoters for antisense RNA expression: mammalian RNA polymerase II, human t-RNA (pol III), and a bacteriophage T7 promoter, (b) the use of two retroviral vector systems: the previously characterized N2 vector, and double ..
  15. RANDOM SINGLE CHAIN ANTIBODY LIBRARY
    JAMES LARRICK; Fiscal Year: 1992
    ..The SC-Ab library will be expressed in a modified lambda vector (lambdal lox) under the control of a bacteriophage T7 promoter which allows IPTG induced production of target proteins in amounts exceeding 50% of total cell ..
  16. NOVEL SYSTEM FOR REGULATED HIGH LEVEL GENE EXPRESSION
    Thomas Fuerst; Fiscal Year: 1992
    ..To this end, the use of regulatory components from the E. coli lactose operon to regulate the expression of bacteriophage T7 RNA polymerase in a single vaccinia virus-based vector system was investigated...
  17. MOLECULAR BASIS OF FUNCTION OF T7 RNA POLYMERASE
    RICHARD IKEDA; Fiscal Year: 1993
    ..An understanding of these rudimentary processes would then allow the study of the more complex processes involved in the regulation of gene expression in bacteriophage T7.
  18. REPLICATION OF T7 DNA
    F Studier; Fiscal Year: 1992
    b>Bacteriophage T7 is a relatively simple, well characterized virus that infects E. coli. It contains a single molecule of linear DNA almost 40,000 base pair long whose complete nucleotide sequence is known...
  19. HYPEREXPRESSION SYSTEM FOR RECOMBINANT BCG VACCINES
    Charles Stover; Fiscal Year: 1992
    ..to develop a BCG vector/host system incorporating BCG stress promotors with transcriptional components from bacteriophage T7 (T7 RNA polymerase) and the E...
  20. RNA POLYMERASE STRUCTURE AND FUNCTION
    William McAllister; Fiscal Year: 2007
    The overall goal of this project is to characterize fundamental aspects of the transcription process using bacteriophage T7 RNA polymerase (RNAP) as a model system, and to interpret this process with regard to the organization and ..
  21. MECHANISMS OF DNA DOUBLE STRAND BREAK REPAIR
    WARREN MASKER; Fiscal Year: 2000
    ..This project will use a model DNA replication system based upon bacteriophage T7 infected Escherichia coli to study the mechanisms by which double strand breaks are repaired...
  22. Structural Studies of the T7 DNA Replisome
    Ying Li; Fiscal Year: 2004
    ..The bacteriophage T7 DNA replication system will be used as the model system in this study...
  23. DNA Damage Clusters: Repair in Mammalian Cells
    Betsy Sutherland; Fiscal Year: 2004
    ..Further, knowledge of conditions affecting formation of clustered damages, and cellular paths for dealing with them could allow the design of more effective radiation therapies. ..
  24. FASEB CONFERENCE: HELICASES: STRUCTURE, FUNCTION
    Smita Patel; Fiscal Year: 2003
    ..This is the second FASEB meeting on this subject and only the fourth meeting dedicated to helicases since discovery of the first helicase about 25 years ago. ..
  25. MECHANISM AND REGULATION OF TRANSCRIPTION INITIATION
    Smita Patel; Fiscal Year: 2006
    ..abstract_text> ..
  26. Elementary Steps in DNA Polymerization
    Kenneth Johnson; Fiscal Year: 2008
    ..abstract_text> ..
  27. KINETIC ANALYSIS OF MICROTUBULE-DEPENDENT ATPASES
    Kenneth Johnson; Fiscal Year: 2003
    ..The combination of approaches outlined here will provide rigorous and direct information to define the structural and mechanistic basis for force production by kinesin. ..
  28. Enhanced Capillary DNA Sequencing
    Philip Serwer; Fiscal Year: 2004
    ..Each adaptation will help solve a current genomic problem, for example, either closing of a partially assembled genome sequence or performing high accuracy analysis of mutations. ..
  29. DNA Packaging Motors
    Philip Serwer; Fiscal Year: 2005
    ..Analysis of bacteriophage DNA packaging motors is expected to provide a basis for understanding the role of motors in disease, especially disease caused by a virus that has a DNA packaging motor. ..
  30. Assembly Visualized Routine Flourescence Microscopy
    Philip Serwer; Fiscal Year: 2008
    ..Applications are anticipated in the analysis of the assembly of disease-causing viruses and determining pathways of oncogenesis. ..
  31. BIOSYNTHESIS OF ENKEPHALIN OPIOID PEPTIDES
    Vivian Hook; Fiscal Year: 2009
    ..These findings will enhance our knowledge of the complexity of the endogenous opioid system. ..
  32. Small Molecular Therapeutics for Friedreich's Ataxia
    Joel M Gottesfeld; Fiscal Year: 2010
    ..These repeats inactivate the gene, and we have developed small molecules that reverse this inactivation by targeting the GAA repeats. ..
  33. 2008 Single Molecule Approaches to Biology Gordon Research Conference
    Taekjip Ha; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  34. Polyamide-Chl inhibition of cancer cell proliferation
    JOEL GOTTESFELD; Fiscal Year: 2009
    ..The mechanism of inhibition of transcription by polyamides will be assessed both in vitro, in cell culture and in animal models...
  35. Poliovirus Receptor and Poliovirus Pathogenesis
    Eckard Wimmer; Fiscal Year: 2009
    ....
  36. HIV-Hepatitis C Virus Interactions and Pathogenesis
    Raymond T Chung; Fiscal Year: 2010
    ..These studies will shed considerable light on HIV-HCV pathogenic interactions and offer a means of productively disrupting these interactions. ..
  37. POMC Processing and Beta-Endorphine-Related Opioid Peptides
    Vivian Y H Hook; Fiscal Year: 2010
    ..New findings from this project will enhance our knowledge of the complexity of biosynthetic mechanisms for endogenous opioid and neuropeptide systems. ..
  38. Structure and Function of IRES Elements
    Eckard Wimmer; Fiscal Year: 2006
    ..We will investigate the molecular reason for this attenuation. Finally, we will determine whether we can exchange viral IRESs also IRESs of cellular mRNA. ..
  39. SMALL MOLECULE REGULATION OF BREAST CANCER EXPRESSION
    JOEL GOTTESFELD; Fiscal Year: 2002
    ..Toxicity of the polyamides will be assessed in cell-based assays and non-specific effects of the polyamides on genome-wide gene expression will be monitored using high density DNA array/hybridization methodology. ..
  40. FASEB CONFERENCE: VIRUS ASSEMBLY
    John Johnson; Fiscal Year: 2002
    ..We also seek to encourage unconventional aspects of virology as represented at our last meeting by presentations describing plant viruses as bio materials and nano reaction vessels. ..
  41. EUROPIC america 2002: a conference for picornaviruses
    Eckard Wimmer; Fiscal Year: 2002
    ..The over will discuss the difficulties (circulation of vaccine strains, recent outbreak of poliomyelitis in the Western Hemisphere) that have emerged during the current, historic effect to globally eradicate poliovirus. ..
  42. CONFERENCE ON DNA REPLICATION AND RECOMBINATION
    KENNETH MARIANS; Fiscal Year: 2002
    ..Given the rapid pace of progress in these fields over the last three years, it should prove to be a timely and exciting meeting. ..
  43. HIGH RESOLUTION OF STRUCTURAL STUDIES OF INSECT VIRUSES
    John Johnson; Fiscal Year: 2001
    ....
  44. Packaging of Ebola Virus RNA into Budding VLPs
    RONALD HARTY; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  45. Evolution of Human C-cluster Enteroviruses
    Eckard Wimmer; Fiscal Year: 2003
    ..To assess this scenario we propose to establish a model system to test possibilities of speciation of C-cluster enteroviruses. ..
  46. Specificity of Protein-DNA/RNA Interactions
    JOEL GOTTESFELD; Fiscal Year: 2004
    ..Thus, understanding the basis for nucleic acid recognition and dimerization specificity may provide the necessary background for development of the novel therapeutic approaches. ..
  47. A universal DNA endonuclease
    Shuang yong Xu; Fiscal Year: 2005
    ..For isothermal applications of the universal nuclease at 50oC-70oC, the nuclease domain will be derived from thermophilic endonucleases. ..
  48. Chemical Genetics:Cellular Regulators of HCV (RMI)
    Raymond Chung; Fiscal Year: 2004
    ..Second, discovery of hit compounds that positively or negatively regulate HCV replication will ultimately help lead to identification of novel cellular targets that can be exploited to further interrupt the viral lifecycle. ..
  49. Regulation of HIV transcription by Tat and P-TEPb
    TSAFI PE ERY; Fiscal Year: 2004
    ..We will explore the ability of HIC and granulin to regulate transactivation of the HIV promoter and define the sequences of these proteins necessary for binding to cyclin T1 and Tat. ..
  50. Compensating Tat/TAR mutations in drug-resistant HIV-1
    TSAFI PE ERY; Fiscal Year: 2005
    ..To sequence and analyze HIV-1 Tat genes and the TAR element of viruses isolated from HIV-1 drug-resistant patients. 2. To characterize the replicative fitness and function of selected Tat and TAR variants. ..
  51. Budding of Negative-sense RNA Viruses
    RONALD HARTY; Fiscal Year: 2006
    ....
  52. Cloning and Engineering of Nicking enzymes
    Shuang yong Xu; Fiscal Year: 2004
    ..abstract_text> ..
  53. IMMUNOGLOBULIN AND T CELL RECEPTOR GENE ASSEMBLY
    David Schatz; Fiscal Year: 2006
    ..Overall, our experiments will address both the targeting and rejoining functions of the RAG proteins, with the long term goal of linking these fundamental activities to chromosomal translocations found in human malignancies. ..
  54. Serpin Protease Inhibitors & Opioid Neuropeptides
    Vivian Hook; Fiscal Year: 2006
    ..Serpin regulation of opioid peptides that mediate analgesia and stress may lead to development of new therapeutic approaches in pain and neurologic disease. ..
  55. Plant Viruses as Platforms for Biomaterials
    John Johnson; Fiscal Year: 2006
    ....
  56. A Genetic System Able to Screen for Active DNA Demethylation
    Shuang yong Xu; Fiscal Year: 2006
    ..In addition, knowledge of the mechanism of demethylation could be crucial in converting fully differentiated adult stem cells into totipotent stem cells. [unreadable] [unreadable] [unreadable]..
  57. Reagents for Preparing Structure-Free DNA and RNA
    Howard Gamper; Fiscal Year: 2007
    ....
  58. INHIBITION OF HIV GENE TRANSCRIPTION BY SMALL MOLECULES
    JOEL GOTTESFELD; Fiscal Year: 2004
    ..Given our success in inhibition of virus replication in cell culture, we propose collaborative preclinical studies with the Mosier laboratory to determine the effectiveness of polyamides in the human PBL-SCID mouse model. ..
  59. PICORNAVIRUS MOLECULAR BIOLOGY
    Eckard Wimmer; Fiscal Year: 2007
    ..Our studies may lead to the development of new[unreadable] vaccines and of novel drugs directed against picornavirus[unreadable] infection.[unreadable]..
  60. Analysis of the Ebola Virus VP24 Channel Protein
    RONALD HARTY; Fiscal Year: 2003
    ..Overall this application will address key mechanisms of Ebola virus pathogenesis that may lead to the development of novel paradigms with direct impact on the development of therapeutic approaches. ..
  61. Histone Deacetylase Inhibitors as Therapeutics for Friedreich's ataxia
    JOEL GOTTESFELD; Fiscal Year: 2007
    ..We are developing small molecules to reverse this effect, with the hope that these molecules may be therapeutic for FRDA. [unreadable] [unreadable] [unreadable]..
  62. HOST IMMUNOMODULATION BY PARASITES
    Ramaswamy Kalyanasundaram; Fiscal Year: 2004
    ..Overall, the findings from these studies we believe might help improve per-cutaneous vaccination against this disease using defined candidate vaccine antigens against schistosomiasis. ..