bacteriophages

Summary

Summary: Viruses whose hosts are bacterial cells.

Top Publications

  1. ncbi CRISPR/Cas, the immune system of bacteria and archaea
    Philippe Horvath
    Danisco France SAS, BP10, F 86220 Dangé Saint Romain, France
    Science 327:167-70. 2010
  2. ncbi The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
    Josiane E Garneau
    Departement de Biochimie, de microbiologie et de bio informatique, Faculte des sciences et de genie, Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Felix d Herelle Reference Center for Bacterial Viruses, Universite Laval, Quebec City, Quebec G1V 0A6, Canada
    Nature 468:67-71. 2010
  3. pmc CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea
    Luciano A Marraffini
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Nat Rev Genet 11:181-90. 2010
  4. ncbi Bacteriophage resistance mechanisms
    Simon J Labrie
    Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Rev Microbiol 8:317-27. 2010
  5. pmc Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion
    Harald Brussow
    Nestle, Research Center, Vers chez les Blanc, CH 1000 Lausanne, Switzerland
    Microbiol Mol Biol Rev 68:560-602, table of contents. 2004
  6. ncbi Virus population dynamics and acquired virus resistance in natural microbial communities
    Anders F Andersson
    Departments of Earth and Planetary Science and Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720, USA
    Science 320:1047-50. 2008
  7. ncbi CRISPR/Cas system and its role in phage-bacteria interactions
    Hélène Deveau
    Departement de Biochimie, Microbiologie et Bio informatique, Faculte des sciences et de genie, Groupe de Recherche en Ecologie Buccale, Universite Laval, Quebec City, Quebec, G1V 0A6, Canada
    Annu Rev Microbiol 64:475-93. 2010
  8. pmc The human gut virome: inter-individual variation and dynamic response to diet
    Samuel Minot
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Genome Res 21:1616-25. 2011
  9. pmc The CRISPR system: small RNA-guided defense in bacteria and archaea
    Fedor V Karginov
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Mol Cell 37:7-19. 2010
  10. ncbi Ecology of prokaryotic viruses
    Markus G Weinbauer
    Department of Biological Oceanography, Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands
    FEMS Microbiol Rev 28:127-81. 2004

Detail Information

Publications291 found, 100 shown here

  1. ncbi CRISPR/Cas, the immune system of bacteria and archaea
    Philippe Horvath
    Danisco France SAS, BP10, F 86220 Dangé Saint Romain, France
    Science 327:167-70. 2010
    ..CRISPR features may be exploited for typing purposes, epidemiological studies, host-virus ecological surveys, building specific immunity against undesirable genetic elements, and enhancing viral resistance in domesticated microbes...
  2. ncbi The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
    Josiane E Garneau
    Departement de Biochimie, de microbiologie et de bio informatique, Faculte des sciences et de genie, Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Felix d Herelle Reference Center for Bacterial Viruses, Universite Laval, Quebec City, Quebec G1V 0A6, Canada
    Nature 468:67-71. 2010
    ..It was recently demonstrated that these variable loci can incorporate spacers from infecting bacteriophages and then provide immunity against subsequent bacteriophage infections in a sequence-specific manner...
  3. pmc CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea
    Luciano A Marraffini
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Nat Rev Genet 11:181-90. 2010
    ..repeats (CRISPRs) are involved in a more recently discovered interference pathway that protects cells from bacteriophages and conjugative plasmids...
  4. ncbi Bacteriophage resistance mechanisms
    Simon J Labrie
    Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Rev Microbiol 8:317-27. 2010
    ..In this Review, we highlight the most important antiviral mechanisms of bacteria as well as the counter-attacks used by phages to evade these systems...
  5. pmc Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion
    Harald Brussow
    Nestle, Research Center, Vers chez les Blanc, CH 1000 Lausanne, Switzerland
    Microbiol Mol Biol Rev 68:560-602, table of contents. 2004
    Comparative genomics demonstrated that the chromosomes from bacteria and their viruses (bacteriophages) are coevolving...
  6. ncbi Virus population dynamics and acquired virus resistance in natural microbial communities
    Anders F Andersson
    Departments of Earth and Planetary Science and Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720, USA
    Science 320:1047-50. 2008
    ..Only the most recently acquired spacers match coexisting viruses, which suggests that community stability is achieved by rapid but compensatory shifts in host resistance levels and virus population structure...
  7. ncbi CRISPR/Cas system and its role in phage-bacteria interactions
    Hélène Deveau
    Departement de Biochimie, Microbiologie et Bio informatique, Faculte des sciences et de genie, Groupe de Recherche en Ecologie Buccale, Universite Laval, Quebec City, Quebec, G1V 0A6, Canada
    Annu Rev Microbiol 64:475-93. 2010
    ..This review describes the recent progress made in this fast-expanding field...
  8. pmc The human gut virome: inter-individual variation and dynamic response to diet
    Samuel Minot
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Genome Res 21:1616-25. 2011
    ..Thus these data provide an overview of the composition of the human gut virome and associate virome structure with diet...
  9. pmc The CRISPR system: small RNA-guided defense in bacteria and archaea
    Fedor V Karginov
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Mol Cell 37:7-19. 2010
    ..Here we review the inner workings of this adaptable and heritable immune system and draw comparisons to small RNA-guided defense mechanisms in eukaryotic cells...
  10. ncbi Ecology of prokaryotic viruses
    Markus G Weinbauer
    Department of Biological Oceanography, Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands
    FEMS Microbiol Rev 28:127-81. 2004
    ..Genomics or proteomics are providing us now with powerful tools in phage ecology, but final testing will have to be performed in the environment...
  11. pmc Viruses in the faecal microbiota of monozygotic twins and their mothers
    Alejandro Reyes
    Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, Missouri 63108, USA
    Nature 466:334-8. 2010
    ..These results indicate that a predatory viral-microbial dynamic, manifest in a number of other characterized environmental ecosystems, is notably absent in the very distal intestine...
  12. ncbi Viral metagenomics
    Robert A Edwards
    Department of Biology, Center for Microbial Sciences, San Diego State University, 5500 Campanile Drive, San Diego, California 92182, USA
    Nat Rev Microbiol 3:504-10. 2005
    ..Metagenomic analyses of uncultured viral communities circumvent these limitations and can provide insights into the composition and structure of environmental viral communities...
  13. ncbi Rapidly evolving CRISPRs implicated in acquired resistance of microorganisms to viruses
    Gene W Tyson
    Department of Environmental Science, Policy and Management, University of California, Berkeley, Berkeley, CA 94720, USA
    Environ Microbiol 10:200-7. 2008
    ..Results reinforce the importance of phage-host interactions in shaping microbial ecology and evolution over very short time scales...
  14. ncbi CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies
    C Pourcel
    GPMS, Institut de Genetique et Microbiologie, Universite Paris XI, 91405 Orsay Cedex, France
    Microbiology 151:653-63. 2005
    ..This is believed to be the first time that the origin of the spacers in CRISPR elements has been explained. The CRISPR structure provides a new and robust identification tool...
  15. ncbi Phage abortive infection in lactococci: variations on a theme
    Marie Christine Chopin
    Unité de Génétique Microbienne, INRA, CRJJ, 78352 Jouy en Josas Cedex, France
    Curr Opin Microbiol 8:473-9. 2005
    ..Abis are widespread in bacteria, and recent analysis indicates that Abis might have additional roles other than conferring phage resistance...
  16. pmc Antagonistic coevolution accelerates molecular evolution
    Steve Paterson
    School of Biological Sciences, Biosciences Building, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK
    Nature 464:275-8. 2010
    ..These results demonstrate, at both the genomic and phenotypic level, that antagonistic coevolution is a cause of rapid and divergent evolution, and is likely to be a major driver of evolutionary change within species...
  17. pmc The phage-host arms race: shaping the evolution of microbes
    Adi Stern
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Bioessays 33:43-51. 2011
    ..The commonalities between defense mechanisms suggest avenues for the discovery of novel forms of these mechanisms based on their evolutionary traits...
  18. pmc Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin
    Petr G Leiman
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47906, USA
    Proc Natl Acad Sci U S A 106:4154-9. 2009
    ..We propose that T6SS is a multicomponent structure whose extracellular part resembles both structurally and functionally a bacteriophage tail, an efficient machine that translocates proteins and DNA across lipid membranes into cells...
  19. pmc Bacteriophage endolysins: a novel anti-infective to control Gram-positive pathogens
    Vincent A Fischetti
    Laboratory of Bacterial Pathogenesis, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Int J Med Microbiol 300:357-62. 2010
    ..Here we review the current literature showing the effectiveness of these enzymes in controlling a variety of infections...
  20. ncbi Explaining microbial population genomics through phage predation
    Francisco Rodriguez-Valera
    Departmento de Producción Vegetal y Microbiología, Universidad Miguel Hernandez, San Juan de Alicante, Spain
    Nat Rev Microbiol 7:828-36. 2009
    ..Periodic selection and phage predation dynamics are not mutually exclusive; we compare their predictions to shed light on the ecological circumstances under which each type of dynamics could predominate...
  21. pmc Nasty viruses, costly plasmids, population dynamics, and the conditions for establishing and maintaining CRISPR-mediated adaptive immunity in bacteria
    Bruce R Levin
    Department of Biology, Emory University, Atlanta, Georgia, United States of America
    PLoS Genet 6:e1001171. 2010
    ..I suggest protocols for estimating these parameters and outline the design of experiments to evaluate the validity of these models and test these hypotheses...
  22. ncbi Structural changes induced by a lytic bacteriophage make ciprofloxacin effective against older biofilm of Klebsiella pneumoniae
    Vivek Verma
    Department of Microbiology, Basic Medical Sciences BMS Block, Panjab University, Chandigarh, India
    Biofouling 26:729-37. 2010
    ..However, application of phage and antibiotic in combination resulted in slightly increased biofilm eradication confirming the speculation that antibiotic efficacy can be augmented by bacteriophage...
  23. ncbi Host-parasite coevolutionary arms races give way to fluctuating selection
    Alex R Hall
    Department of Zoology, University of Oxford, Oxford OX1 3PS, UK
    Ecol Lett 14:635-42. 2011
    ..Given that costs of generalism are reported for many other systems, arms races may generally give way to fluctuating selection in antagonistically coevolving populations...
  24. ncbi A bacteriolytic agent that detects and kills Bacillus anthracis
    Raymond Schuch
    Laboratory of Bacteriql Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021, USA
    Nature 418:884-9. 2002
    ..The lytic specificity of PlyG was also exploited as part of a rapid method for the identification of B. anthracis. We conclude that PlyG is a tool for the treatment and detection of B. anthracis...
  25. ncbi Whole genome sequencing of meticillin-resistant Staphylococcus aureus
    M Kuroda
    Hiramatsu, Department of Bacteriology, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan
    Lancet 357:1225-40. 2001
    ..Investigation of many newly identified gene products, including the 70 putative virulence factors, will greatly improve our understanding of the biology of staphylococci and the processes of infectious diseases caused by S aureus...
  26. pmc Engineered bacteriophage targeting gene networks as adjuvants for antibiotic therapy
    Timothy K Lu
    Harvard Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 106:4629-34. 2009
    ..This work establishes a synthetic biology platform for the rapid translation and integration of identified targets into effective antibiotic adjuvants...
  27. ncbi Community genomics among stratified microbial assemblages in the ocean's interior
    Edward F DeLong
    Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 311:496-503. 2006
    ..Comparative genomic analyses of stratified microbial communities have the potential to provide significant insight into higher-order community organization and dynamics...
  28. ncbi Environmental genome shotgun sequencing of the Sargasso Sea
    J Craig Venter
    Institute for Biological Energy Alternatives, 1901 Research Boulevard, Rockville, MD 20850, USA
    Science 304:66-74. 2004
    ..2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity...
  29. ncbi Bacteriophage endolysins--current state of research and applications
    Martin J Loessner
    Institute of Food Science and Nutrition, Swiss Federal Institute of Technology ETH, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    Curr Opin Microbiol 8:480-7. 2005
    ..Clearly, phage endolysins represent great tools for use in molecular biology, biotechnology and in medicine, and we are just beginning to tap this potential...
  30. pmc The phage abortive infection system, ToxIN, functions as a protein-RNA toxin-antitoxin pair
    Peter C Fineran
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom
    Proc Natl Acad Sci U S A 106:894-9. 2009
    ..This is the first demonstration of a novel mechanistic class of TA systems and of an Abi system functioning in different bacterial genera, both with implications for the dynamics of phage-bacterial interactions...
  31. pmc Probing individual environmental bacteria for viruses by using microfluidic digital PCR
    Arbel D Tadmor
    Department of Biochemistry and Molecular Biophysics, California Institute of Technology, Pasadena, CA 91125, USA
    Science 333:58-62. 2011
    ..Our approach does not require culturing hosts or viruses and provides a method for examining virus-bacterium interactions in many environments...
  32. pmc Genomics of bacterial and archaeal viruses: dynamics within the prokaryotic virosphere
    Mart Krupovic
    Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Departement de Microbiologie, 25 rue du Dr Roux, 75015 Paris, France
    Microbiol Mol Biol Rev 75:610-35. 2011
    ..majority of characterized prokaryotic viruses belong to the order Caudovirales, double-stranded DNA (dsDNA) bacteriophages with tails...
  33. pmc Targeted bacterial immunity buffers phage diversity
    Jan O Haerter
    Center for Models of Life, Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, 2100 Copenhagen Ø, Denmark
    J Virol 85:10554-60. 2011
    ..Coexistence is predicted to be a consequence of the presence of many interdependent species that stress but do not overrun the bacterial defense system...
  34. pmc Virulent bacteriophage for efficient biocontrol of Listeria monocytogenes in ready-to-eat foods
    Susanne Guenther
    Institute of Food Science and Nutrition, ETH Zurich, Schmelzbergstrasse 7, LFV B20, 8092 Zurich, Switzerland
    Appl Environ Microbiol 75:93-100. 2009
    ..b>Bacteriophages are natural enemies of bacteria and are suitable candidates for the environmentally friendly biocontrol of ..
  35. pmc Pulmonary bacteriophage therapy on Pseudomonas aeruginosa cystic fibrosis strains: first steps towards treatment and prevention
    Eric Morello
    Molecular Biology of the Gene in Extremophiles Unit, Department of Microbiology, Institut Pasteur, Paris, France
    PLoS ONE 6:e16963. 2011
    ..New bacteriophages were isolated from environmental samples and characterized...
  36. ncbi Innate and adaptive immunity in bacteria: mechanisms of programmed genetic variation to fight bacteriophages
    David Bikard
    Laboratory of Bacteriology, The Rockefeller University, 10065 New York, NY, USA
    Curr Opin Immunol 24:15-20. 2012
    Bacteria are constantly challenged by bacteriophages (viruses that infect bacteria), the most abundant microorganism on earth. Bacteria have evolved a variety of immunity mechanisms to resist bacteriophage infection...
  37. ncbi Recent advances in bacteriophage therapy: how delivery routes, formulation, concentration and timing influence the success of phage therapy
    Elizabeth M Ryan
    School of Pharmacy, Queens University Belfast, Belfast, UK
    J Pharm Pharmacol 63:1253-64. 2011
    b>Bacteriophages are bacteria-specific viruses that infect and, in the case of obligately lytic phages, destroy their host bacteria. Phage therapy has been used therapeutically to combat bacterial infections since their discovery...
  38. ncbi Application of bacteriophages for detection and control of foodborne pathogens
    Steven Hagens
    Institute of Food Science and Nutrition, ETH Zurich, Schmelzbergstrasse 7, CH 8092, Zurich, Switzerland
    Appl Microbiol Biotechnol 76:513-9. 2007
    ..This review intends to briefly summarize and explain the principles and current standing of these approaches...
  39. pmc Different classes of antibiotics differentially influence shiga toxin production
    Colleen Marie McGannon
    Citizen Schools, Albuquerque, New Mexico 87110, USA
    Antimicrob Agents Chemother 54:3790-8. 2010
    ..Remarkably, very high levels of Stx were detected even when growth of O157:H7 was completely suppressed by CIP. In contrast, azithromycin significantly reduced Stx levels even when O157:H7 viability remained high...
  40. pmc Complete WO phage sequences reveal their dynamic evolutionary trajectories and putative functional elements required for integration into the Wolbachia genome
    Kohjiro Tanaka
    National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba 305 8566, Japan
    Appl Environ Microbiol 75:5676-86. 2009
    ..Here, we report the genome and structure of active bacteriophages from a Wolbachia endosymbiont...
  41. ncbi Bacteriophage lytic enzymes: novel anti-infectives
    Vincent A Fischetti
    Laboratory of Bacterial Pathogenesis, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Trends Microbiol 13:491-6. 2005
    ..Thus, lysins could be an effective anti-infective in an age of mounting antibiotic resistance...
  42. pmc Toxin gene expression by shiga toxin-producing Escherichia coli: the role of antibiotics and the bacterial SOS response
    P T Kimmitt
    The Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom
    Emerg Infect Dis 6:458-65. 2000
    ..These features and the use of SOS-inducing antibiotics in clinical practice and animal husbandry may account for the recent emergence of STEC disease...
  43. pmc Prevalence, conservation and functional analysis of Yersinia and Escherichia CRISPR regions in clinical Pseudomonas aeruginosa isolates
    K C Cady
    Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
    Microbiology 157:430-7. 2011
    ..This work provides both the first examination to our knowledge of CRISPR regions within clinical P. aeruginosa isolates and a collection of defined CRISPR-positive and -negative strains for further CRISPR and cas gene studies...
  44. pmc Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes
    Terry Fei Fan Ng
    College of Marine Science, University of South Florida, St Petersburg, Florida, United States of America
    PLoS ONE 6:e20579. 2011
    ..Numerous bacteriophages and insect viruses were also detected, including a novel densovirus likely infecting Culex erythrothorax...
  45. pmc Metagenomic analysis of the viral communities in fermented foods
    Eun Jin Park
    Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130 701, Republic of Korea
    Appl Environ Microbiol 77:1284-91. 2011
    ..There were some discrepancies between the prediction of bacteriophages hosts via homology comparison and bacterial distribution, as determined from 16S rRNA gene sequencing...
  46. pmc Termination and antitermination: RNA polymerase runs a stop sign
    Thomas J Santangelo
    Department of Microbiology and The RNA Group, The Ohio State University, Columbus, Ohio 43210, USA
    Nat Rev Microbiol 9:319-29. 2011
    ..This Review discusses the actions of bacterial and phage antiterminators that allow RNA polymerase to override a terminator when the circumstances demand it...
  47. pmc Comparative metagenomics of microbial traits within oceanic viral communities
    Itai Sharon
    Faculty of Biology, Technion, Israel Institute of Technology, Haifa, Israel
    ISME J 5:1178-90. 2011
    ....
  48. pmc Multiscale model of CRISPR-induced coevolutionary dynamics: diversification at the interface of Lamarck and Darwin
    Lauren M Childs
    School of Biology and School of Mathematics, Georgia Institute of Technology, 310 Ferst Dr, Atlanta, Georgia 30332, USA
    Evolution 66:2015-29. 2012
    ..We close by discussing how our explicit eco-evolutionary model of CRISPR immunity can help guide efforts to understand the drivers of diversity seen in microbial communities where CRISPR systems are active...
  49. pmc Metagenomic detection of phage-encoded platelet-binding factors in the human oral cavity
    Dana Willner
    Department of Biology, San Diego State University, San Diego, CA 92182, USA
    Proc Natl Acad Sci U S A 108:4547-53. 2011
    ..Our results indicate that the oral cavity is a reservoir for pblA and pblB genes and for phage SM1 itself. Further studies will determine the association between pblA and pblB genes in the oral cavity and the risk of endocarditis...
  50. pmc Prokaryotic homologs of Argonaute proteins are predicted to function as key components of a novel system of defense against mobile genetic elements
    Kira S Makarova
    National Center for Biotechnology Information, NLM, National Institutes of Health, Bethesda, Maryland 20894, USA
    Biol Direct 4:29. 2009
    ..Many prokaryotes also encode homologs of Argonaute-PIWI proteins but their functions remain unknown...
  51. pmc Bacteriophage lysins as effective antibacterials
    Vincent A Fischetti
    Laboratory of Bacterial Pathogenesis, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Curr Opin Microbiol 11:393-400. 2008
    ..Thus, lysins may be a much needed anti-infective in an age of mounting antibiotic resistance...
  52. ncbi Current insights into phage biodiversity and biogeography
    Rebecca Vega Thurber
    Florida International University, Department of Biological Sciences, Biscayne Bay Campus, Marine Science 355, 3000 NE 151st Street, North Miami, FL 33181, USA
    Curr Opin Microbiol 12:582-7. 2009
    ..This review discusses currents in the field of phage ecology with particular focus on efforts to characterize phage diversity and biogeography across various spatial and temporal scales...
  53. pmc Identification of the receptor-binding protein in 936-species lactococcal bacteriophages
    Kitt Dupont
    Department of Food Science, The Royal Veterinary and Agricultural University, DK 1958 Frederiksberg C, Denmark
    Appl Environ Microbiol 70:5818-24. 2004
    ..Interestingly, sk1-like phages bind to and infect a defined group of L. lactis subsp. cremoris strains, while bIL170-like phages bind to and infect a defined group of L. lactis subsp. lactis strains...
  54. pmc Rapid diversification of coevolving marine Synechococcus and a virus
    Marcia F Marston
    Department of Biology and Marine Biology, Roger Williams University, Bristol, RI 02809, USA
    Proc Natl Acad Sci U S A 109:4544-9. 2012
    ..of the viral candidates were tail fiber genes, thought to be the primary determinants of host range in tailed bacteriophages, highlighting the difficulty in generalizing results from bacteriophage infecting γ-Proteobacteria...
  55. ncbi Muralytic activity and modular structure of the endolysins of Pseudomonas aeruginosa bacteriophages phiKZ and EL
    Yves Briers
    Division of Gene Technology, Department of Biosystems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B 3001 Leuven, Belgium
    Mol Microbiol 65:1334-44. 2007
    ..Both KZ144 and EL188 appear to be a natural chimeric enzyme, originating from a recombination of a cell wall-binding domain encoded by a Bacillus or Clostridium species and a catalytic domain of an unknown ancestor...
  56. ncbi Reticulate representation of evolutionary and functional relationships between phage genomes
    Gipsi Lima-Mendez
    Service de Bioinformatique des Génomes et des Réseaux BiGRe, Universite Libre de Bruxelles, Bruxelles, Belgium
    Mol Biol Evol 25:762-77. 2008
    ..These 2 classification schemes provide an automatic and dynamic way of representing the relationships within the phage population and can be extended to include newly sequenced phage genomes, as well as other types of genetic elements...
  57. ncbi Phage as agents of lateral gene transfer
    Carlos Canchaya
    Nestle Research Centre, CH 1000 Lausanne 26, Vers chez les Blanc, Switzerland
    Curr Opin Microbiol 6:417-24. 2003
    ..Occasionally, phages are also involved in the lateral transfer of other mobile DNA elements or bacterial DNA. Recent advances in the field of genomics have revealed a major impact by phages on bacterial chromosome evolution...
  58. pmc Complete bacteriophage transfer in a bacterial endosymbiont (Wolbachia) determined by targeted genome capture
    Bethany N Kent
    Department of Biological Sciences, Vanderbilt University, USA
    Genome Biol Evol 3:209-18. 2011
    ..To test whether bacteriophages transfer as single genes or larger regions between coinfections, we sequenced the genome of the obligate ..
  59. pmc Phage WO of Wolbachia: lambda of the endosymbiont world
    Bethany N Kent
    Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA
    Trends Microbiol 18:173-81. 2010
    ..In this sense, phage WO might be likened to phage lambda of the endosymbiont world...
  60. pmc Shiga toxin as a bacterial defense against a eukaryotic predator, Tetrahymena thermophila
    William Lainhart
    Department of Biological Sciences, University at Buffalo, Cooke Hall, North Campus, Buffalo, NY 14260, USA
    J Bacteriol 191:5116-22. 2009
    ..The genes that encode these exotoxins are commonly found in bacterial viruses (bacteriophages)...
  61. ncbi Phage regulatory circuits and virulence gene expression
    Matthew K Waldor
    Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, Boston, MA 02111, USA
    Curr Opin Microbiol 8:459-65. 2005
    ..Clearly bacteriophages are important vectors for disseminating virulence genes, but, in addition, do phage regulatory circuits ..
  62. pmc Viruses' life history: towards a mechanistic basis of a trade-off between survival and reproduction among phages
    Marianne De Paepe
    Laboratoire de Genetique Moleculaire, Evolutive et Medicale, University of Paris 5, INSERM, Paris, France
    PLoS Biol 4:e193. 2006
    ..Moreover, such a positive correlation between mortality and multiplication reveals an underexplored trade-off in host-parasite interactions...
  63. ncbi Taking aim on bacterial pathogens: from phage therapy to enzybiotics
    Juan A Hermoso
    Grupo de Cristalografia Macromolecular y Biologia Estructural, Instituto Quimica Fisica Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain
    Curr Opin Microbiol 10:461-72. 2007
    The bactericidal activity of bacteriophages has been used to treat human infections for years as an alternative or a complement to antibiotic therapy...
  64. ncbi Phages will out: strategies of host cell lysis
    I Young
    Dept of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
    Trends Microbiol 8:120-8. 2000
    ..By contrast, lytic single-stranded RNA and DNA phages accomplish lysis by producing a single lysis protein without muralytic activity...
  65. ncbi CRISPR--a widespread system that provides acquired resistance against phages in bacteria and archaea
    Rotem Sorek
    Rotem Sorek, Victor Kunin and Philip Hugenholtz are at the Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, California 94598, USA
    Nat Rev Microbiol 6:181-6. 2008
    ..CRISPR arrays, together with a group of associated proteins, confer resistance to phages, possibly by an RNA-interference-like mechanism. This Progress discusses the structure and function of this newly recognized antiviral mechanism...
  66. pmc Interaction between bacteriophage DMS3 and host CRISPR region inhibits group behaviors of Pseudomonas aeruginosa
    Michael E Zegans
    Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
    J Bacteriol 191:210-9. 2009
    ..Our observations suggest a role for CRISPR regions in modifying the effects of lysogeny on P. aeruginosa...
  67. ncbi Preventing biofilms of clinically relevant organisms using bacteriophage
    Rodney M Donlan
    Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Mail Stop C 16, 1600 Clifton Road, N E, Atlanta, GA 30333, USA
    Trends Microbiol 17:66-72. 2009
    ..Phage mixtures or engineered phages could provide effective strategies to overcome these obstacles. Lytic bacteriophages could become a new class of anti-biofilm agents.
  68. pmc Quality-controlled small-scale production of a well-defined bacteriophage cocktail for use in human clinical trials
    Maya Merabishvili
    Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia
    PLoS ONE 4:e4944. 2009
    ..small-scale, laboratory-based, production and quality control of a cocktail, consisting of exclusively lytic bacteriophages, designed for the treatment of Pseudomonas aeruginosa and Staphylococcus aureus infections in burn wound ..
  69. ncbi Biodiversity and biogeography of phages in modern stromatolites and thrombolites
    Christelle Desnues
    Department of Biology, San Diego State University, San Diego, California 92182, USA
    Nature 452:340-3. 2008
    ..Taken together, these results prove that viruses in modern microbialites display biogeographical variability and suggest that they may be derived from an ancient community...
  70. ncbi Phage integrases: biology and applications
    Amy C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    J Mol Biol 335:667-78. 2004
    ..Directed evolution can be used to increase further the affinity of an integrase for a particular native sequence, opening up additional applications for genomic modification...
  71. ncbi Bacteriophages as potential new therapeutics to replace or supplement antibiotics
    Mzia Kutateladze
    G Eliava Institute of Bacteriophages, Microbiology and Virology, Tbilisi, Georgia, USA
    Trends Biotechnol 28:591-5. 2010
    Over recent decades, a growing body of literature has validated the use of bacteriophages for therapy and prophylaxis in the war against drug-resistant bacteria...
  72. pmc Clostridium perfringens bacteriophages ΦCP39O and ΦCP26F: genomic organization and proteomic analysis of the virions
    Bruce S Seal
    Richard B Russell Agricultural Research Center, USDA, Athens, GA 30605, USA
    Arch Virol 156:25-35. 2011
    ..material, sewage and poultry processing drainage water were screened for virulent Clostridium perfringens bacteriophages. Viruses isolated from broiler chicken offal washes (O) and poultry feces (F), designated ΦCP39O and ΦCP26F, ..
  73. pmc Microbiological and molecular assessment of bacteriophage ISP for the control of Staphylococcus aureus
    Katrien Vandersteegen
    Division of Gene Technology, Katholieke Universiteit Leuven, Heverlee, Belgium
    PLoS ONE 6:e24418. 2011
    ..No toxic or virulence-associated proteins were observed. The microbiological and molecular characterization of ISP supports its application in a phage cocktail for therapeutic purposes...
  74. ncbi Determination of bacteriophage genome size by pulsed-field gel electrophoresis
    Erika Lingohr
    Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada
    Methods Mol Biol 502:19-25. 2009
    ..2 to 40-50 kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE), which extends the range of molecular separation from 200 bp to 12 Mb...
  75. ncbi A modular view of the bacteriophage genomic space: identification of host and lifestyle marker modules
    Gipsi Lima-Mendez
    Department of Applied Biological Sciences, Faculty of Sciences, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium
    Res Microbiol 162:737-46. 2011
    ..e. novel module combinations) and obtain insight into the host range for this particular group, highlighting the utility of prophage prediction to better characterize phage diversity...
  76. ncbi Bacteriophage therapy: a revitalized therapy against bacterial infectious diseases
    Shigenobu Matsuzaki
    Department of Molecular Microbiology and Infections, Kochi Medical School, Kohasu, Nankoku, Japan
    J Infect Chemother 11:211-9. 2005
    ..Phage therapy will compensate for unavoidable complications of chemotherapy such as the appearance of multidrug resistance or substituted microbism...
  77. ncbi Coevolution with viruses drives the evolution of bacterial mutation rates
    Csaba Pal
    Department of Zoology, University of Oxford, Oxford OX1 3PS, UK
    Nature 450:1079-81. 2007
    ..Given their ubiquity, bacteriophages may play an important role in the evolution of bacterial mutation rates.
  78. pmc Genotypic and phenotypic diversity among induced, stx2-carrying bacteriophages from environmental Escherichia coli strains
    Cristina Garcia-Aljaro
    Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain
    Appl Environ Microbiol 75:329-36. 2009
    Shiga toxin 2 (stx(2)) gene-carrying bacteriophages have been shown to convert Escherichia coli strains to Shiga toxin-producing Escherichia coli (STEC). In this study, 79 E...
  79. ncbi Bacteriophages as twenty-first century antibacterial tools for food and medicine
    Damien Maura
    Institut Pasteur, Molecular Biology of the Gene in Extremophiles Unit, Department of Microbiology, 75015 Paris, France
    Appl Microbiol Biotechnol 90:851-9. 2011
    ..Within these contexts, studies on the use of bacteriophages in both medicine and the food industry have recently flourished...
  80. ncbi Viral control of bacterial biodiversity--evidence from a nutrient-enriched marine mesocosm experiment
    Ruth Anne Sandaa
    University of Bergen, PO Box 7800, Department of Biology, N 5020 Bergen, Norway
    Environ Microbiol 11:2585-97. 2009
    ....
  81. ncbi Bacteriophage flux in endosymbionts (Wolbachia): infection frequency, lateral transfer, and recombination rates
    Seth R Bordenstein
    The Marine Biological Laboratory, Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Woods Hole, Massachusetts, USA
    Mol Biol Evol 21:1981-91. 2004
    ..endosymbionts typically lack one of the major contributors of genomic flux in the free-living microbial world-bacteriophages. This study yields three results that show bacteriophages have, to the contrary, been influential in the ..
  82. ncbi Bacteriophage therapy: exploiting smaller fleas
    Stan Deresinski
    Department of Medicine, Division of Infectious Disease and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA
    Clin Infect Dis 48:1096-101. 2009
    Although bacteriophages have been used for the treatment of patients with bacterial infections in some regions of the world for >9 decades, adequate clinical trials of the safety and efficacy of the treatment have not been reported...
  83. pmc ACLAME: a CLAssification of Mobile genetic Elements, update 2010
    Raphael Leplae
    Bioinformatique des Génomes et des Réseaux, Universite Libre de Bruxelles, Boulevard du Triomphe, 1050 Bruxelles, Belgium
    Nucleic Acids Res 38:D57-61. 2010
    ..ulb.ac.be). The BLAST interface for querying the database has been extended and numerous tools for in-depth analysis of the results have been added...
  84. ncbi Epitope identification and discovery using phage display libraries: applications in vaccine development and diagnostics
    Lin Fa Wang
    CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong 3220, Victoria, Australia
    Curr Drug Targets 5:1-15. 2004
    ..Instead, this review aims to focus on specific applications related to the discovery and identification of epitopes which have potential as vaccine candidates or can be used in disease diagnosis...
  85. ncbi Isolation and characterization of the bacteriophage WO from Wolbachia, an arthropod endosymbiont
    Yukiko Fujii
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biochem Biophys Res Commun 317:1183-8. 2004
    ..This bacteriophage is the only known mobile genetic element potentially used for transformation of Wolbachia...
  86. ncbi The bacteriophage DNA packaging motor
    Venigalla B Rao
    Department of Biology, The Catholic University of America, Washington, D C 20064, USA
    Annu Rev Genet 42:647-81. 2008
    An ATP-powered DNA translocation machine encapsidates the viral genome in the large dsDNA bacteriophages. The essential components include the empty shell, prohead, and the packaging enzyme, terminase...
  87. ncbi Photosynthesis genes in marine viruses yield proteins during host infection
    Debbie Lindell
    Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 438:86-9. 2005
    ..We thus propose that the phage genes are functional in photosynthesis and that they may be increasing phage fitness by supplementing the host production of these proteins...
  88. pmc Bacteriophage genomics
    Graham F Hatfull
    Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Curr Opin Microbiol 11:447-53. 2008
    ..With an estimated 10(31) bacterial and archael viruses in the biosphere, our view of the virosphere will draw into sharper focus as further bacteriophage genomes are characterized...
  89. pmc Analysis of high-throughput sequencing and annotation strategies for phage genomes
    Matthew R Henn
    The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, United States of America
    PLoS ONE 5:e9083. 2010
    ..Of the approximately 550 phage genomes currently available in the public domain, fewer than 5% are marine phage...
  90. pmc Diversity among the tailed-bacteriophages that infect the Enterobacteriaceae
    Sherwood R Casjens
    Department of Pathology, Room 5200K EEJMRB, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Res Microbiol 159:340-8. 2008
    ..More recent horizontal exchange of phage tail fiber and early proteins between the categories occurs, but is probably not extremely rapid...
  91. ncbi Bacteriophage observations and evolution
    H W Ackermann
    Department of Medical Biology, Faculty of Medicine, Laval University, QC G1K 7P4, Quebec, Canada
    Res Microbiol 154:245-51. 2003
    b>Bacteriophages are classified into one order and 13 families. Over 5100 phages have been examined in the electron microscope since 1959. At least 4950 phages (96%) are tailed. They constitute the order Caudovirales and three families...
  92. ncbi Bacteriophage therapy
    W C Summers
    Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Annu Rev Microbiol 55:437-51. 2001
    In 1917, bacteriophages were recognized as epizootic infections of bacteria and were almost immediately deployed for antibacterial therapy and prophylaxis...
  93. pmc Genomes of the T4-related bacteriophages as windows on microbial genome evolution
    Vasiliy M Petrov
    Department of Biochemistry, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA, USA
    Virol J 7:292. 2010
    The T4-related bacteriophages are a group of bacterial viruses that share morphological similarities and genetic homologies with the well-studied Escherichia coli phage T4, but that diverge from T4 and each other by a number of ..
  94. pmc The tripartite associations between bacteriophage, Wolbachia, and arthropods
    Seth R Bordenstein
    Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, The Marine Biological Laboratory, Woods Hole, Massachusetts, USA
    PLoS Pathog 2:e43. 2006
    ..Clarifying the roles of lytic and lysogenic phage development in Wolbachia biology will effectively structure inquiries into this research topic...
  95. pmc Genomic and functional analyses of Rhodococcus equi phages ReqiPepy6, ReqiPoco6, ReqiPine5, and ReqiDocB7
    E J Summer
    Center for Phage Technology, Texas A and M University, College Station, TX 77843 2128, USA
    Appl Environ Microbiol 77:669-83. 2011
    ..Application of DocB7 lysates to soils amended with a host strain of R. equi reduced recoverable bacterial CFU, suggesting that phage may be useful in limiting R. equi load in the environment while foals are susceptible to infection...
  96. ncbi Phages across the biosphere: contrasts of viruses in soil and aquatic environments
    Sharath Srinivasiah
    University of Delaware, Delaware Biotechnology Institute, 15 Innovation Way Newark, DE 19711, USA
    Res Microbiol 159:349-57. 2008
    ..This review briefly summarizes recent investigations of viral abundance and diversity in soil environments...
  97. ncbi Phage display: selecting straws instead of a needle from a haystack
    Miha Vodnik
    Department of Pharmaceutical Biology, Faculty of Pharmacy, Askerceva 7, Ljubljana, Slovenia
    Molecules 16:790-817. 2011
    ..This article thoroughly reviews and discusses already identified and new target-unrelated peptides and suggests strategies to avoid their isolation...
  98. ncbi Phage T5 straight tail fiber is a multifunctional protein acting as a tape measure and carrying fusogenic and muralytic activities
    Pascale Boulanger
    Institut de Biochimie et Biophysique Moleculaire et Cellulaire, Universite Paris Sud, Orsay, France
    J Biol Chem 283:13556-64. 2008
    ..The C-terminal region would gain access to the host envelope, permitting the local degradation of the peptidoglycan and the formation of the DNA pore by fusion of the two membranes...
  99. ncbi Therapeutic use of bacteriophages
    James Soothill
    Lancet Infect Dis 4:544-5. 2004
  100. ncbi Basic phage electron microscopy
    Hans W Ackermann
    Department of Medical Microbiology, Faculty of Medicine, Laval University, Quebec, QC, Canada
    Methods Mol Biol 501:113-26. 2009
    ..The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages. Particular problems are encountered in photography, calibration of magnification, measurements, and interpretation of artifacts...
  101. ncbi Bacterial viruses as human vaccines?
    Jason R Clark
    Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, Scotland, UK
    Expert Rev Vaccines 3:463-76. 2004
    b>Bacteriophages (or phages) are viruses of bacteria, consisting of nucleic acid packaged within a protein coat...

Research Grants63

  1. Characterization of new toxins (YmgD and YdfD) from E.coli, targeting cell wall
    Hisako Masuda; Fiscal Year: 2012
    ..The TA systems are now widely found not only on plasmids and on bacteriophages, but also in the chromosome of almost all bacteria. E...
  2. CSHL 2010 Conference on Molecular Genetics and Bacteria & Phages
    David J Stewart; Fiscal Year: 2010
    ..Much as plants and animals have viruses, so bacteria can be infected by bacteriophages that alter their metabolism and biochemistry...
  3. 2012 CSHL Bacteria &Phages Conference
    David J Stewart; Fiscal Year: 2012
    ..Much as plants and animals have viruses, so bacteria can be infected by bacteriophages that alter their metabolism and biochemistry...
  4. Structure and mechanism of CRISPR interference.
    Ailong Ke; Fiscal Year: 2013
    ..combination of CRISPR-associated (Cas) proteins extracts recognizable features from the genomes of viruses (bacteriophages) and plasmids as protospacers that are subsequently incorporated as spacers at the 5'end of genomic ..
  5. Isolation of new phage enzymes to kill B. anthracis
    Vincent A Fischetti; Fiscal Year: 2013
    ..Using a collection of novel bacteriophages isolated from a variety of environments, we showed that stable lysogens of B...
  6. PHAGE T4 HEAD ASSEMBLY AND INITIATION OF INFECTION
    Lindsay W Black; Fiscal Year: 2013
    ..PUBLIC HEALTH RELEVANCE: Viral nucleic acid packaging occurs by a highly conserved mechanism among dsDNA bacteriophages and many DNA viruses of clinical importance including Herpes and Adenoviruses...
  7. Helper phage mobilization of S. aureus superantigen pathogenicity islands
    Gail E Christie; Fiscal Year: 2010
    ..Understanding the mechanism by which these pathogenicity islands are mobilized by staphylococcal bacteriophages will assist in the development of strategies to inhibit the transfer of toxin genes between S...
  8. Structural basis for pathogenicity island mobilization in S. aureus
    Terje Dokland; Fiscal Year: 2013
    ..the emergence of antibiotic-resistant Staphylococcus strains and the renewed interest in therapeutic use of bacteriophages to treat such infections, the phage-induced mobilization of SaPI1 will be an important consideration...
  9. Genome packaging in DNA viruses
    Liang Tang; Fiscal Year: 2013
    ..a key step in morphogenesis of large double-stranded DNA (dsDNA) viruses including tailed double-stranded DNA bacteriophages and herpesviruses, and is essential for assembly of infectious progeny virions...
  10. Microbiome analysis of human oral bacteriophage communities
    DAVID TEVIS PRIDE; Fiscal Year: 2013
    ..Many such viruses are bacteriophages that specifically infect bacteria...
  11. Structural studies of bacteriophage T4 and their potential medical applications
    MICHAEL ROSSMANN; Fiscal Year: 2009
    PROJECT SUMMARY Most bacterial viruses ("bacteriophages" or just "phage") are highly efficient in their ability to infect their often very specific bacterial hosts...
  12. Role of viral and cellular recombination proteins in HSV DNA replication
    Sandra K Weller; Fiscal Year: 2013
    ..that HSV DNA replication is more complex and may involve recombination-dependent replication reminiscent of bacteriophages lambda and T4. We have previously shown that HSV encodes a two-subunit viral recombinase (UL12 and ICP8)...
  13. Gene Silencing by Small RNAs
    JAMES WAVELL AIKEN; Fiscal Year: 2011
    ..to eukaryotes;bacteria utilize small RNAs, notably those made from CRISPR loci that silence the expression of bacteriophages, transposons and plasmids...
  14. Using CRISPR immunity to prevent the spread of virulence traits among pathogens
    Luciano A Marraffini; Fiscal Year: 2012
    ..loci, and their associated cas (CRISPR associated) genes, encode a sequence-specific defense mechanism against bacteriophages and plasmids, the main vehicles of HGT, known as CRISPR interference. CRISPR/Cas systems are extremely diverse...
  15. Generating a library of antimicrobial R-type pyocins against enteric bacteria
    DEAN M SCHOLL; Fiscal Year: 2011
    ..agents can be retargeted to other bacteria by modifying their tail fibers with the related tail proteins of bacteriophages. We therefore hypothesize that R-type pyocins can be used as a versatile platform to target a broad range of ..
  16. Structure and Function of the Herpesvirus Capsid and its DNA-Packaging Machinery
    James F Conway; Fiscal Year: 2013
    ..Parallels with dsDNA bacteriophages suggest that the process of translocating the dsDNA chromosome into the herpesvirus capsid is powered by a ..
  17. Structural studies of bacteriophage T4 and their potential medical applications
    Michael G Rossmann; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Most bacterial viruses ("bacteriophages" or just "phages") are highly efficient in their ability to infect their often very specific bacterial hosts...
  18. MOLECULAR BIOLOGY AND VIRULENCE OF CTX PHAGE
    Matthew K Waldor; Fiscal Year: 2013
    ..Many determinants of virulence and antibiotic resistance are borne by mobile elements, such as plasmids and bacteriophages, which are capable of horizontal transmission...
  19. Engineered R-type pyocins as bactericidal agents against E.coli O157:H7
    DEAN M SCHOLL; Fiscal Year: 2011
    ..agents can be retargeted to other bacteria by modifying their tail fibers with the related tail proteins of bacteriophages. Our leading product candidate, AvR2-V10, consists of the tail fiber of the bacteriophage ?V10 fused to an R2-..
  20. Therapeutic potential of Enterococcus faecalis bacteriophage phiEf11
    Roy H Stevens; Fiscal Year: 2010
    ..faecalis infections is the use of E. faecalis-specific bacterial viruses (bacteriophages) that can lyse and kill cells of these bacteria in the course of a normal lytic infective cycle...
  21. Characterization of the Lysogenic Pathway in P. acnes Bacteriophages
    LAURA JANE MARINELLI; Fiscal Year: 2013
    ..by applicant): The long-term goal of this proposal it to enhance our understanding of acne, in particular the bacteriophages that infect and kill Propionibacterium acnes - a bacterium that contributes to the pathogenesis of the skin ..
  22. Host Genetic Requirements for Viral Infection: A Systems Virology Approach
    NATHANIEL DAVID MAYNARD; Fiscal Year: 2011
    ..interplay between the isc and tus pathways in lambda phage infection and should be relevant to other tailed bacteriophages and mammalian viruses (like HIV) which use "slippery sequences" to control gene expression ratios...
  23. Experimental viral evolution at high mutation rate
    JAMES JEFFREY BULL; Fiscal Year: 2013
    ..Here, viruses (bacteriophages) will be grown in vitro at different levels of mutagenesis and the evolutionary consequences of that ..
  24. Metagenomic study of the human skin microbiome associated with acne
    Huiying Li; Fiscal Year: 2010
    ..These microbes include small eukaryotes, bacteria, viruses and bacteriophages. Third, we will examine the interactions between the microbes and the host by transcriptional profiling of ..
  25. DNA packaging and delivery by dsDNA viruses
    Sherwood R Casjens; Fiscal Year: 2012
    ..The viruses chosen for this study, the tailed-bacteriophages, have direct medical importance since they are the most abundant `life form'on Earth and have huge ..
  26. Deriving P. acnes Bacteriophages from Skin for Acne Therapy
    Robert L Modlin; Fiscal Year: 2012
    ..We hypothesize that P. acnes bacteriophages, and/or their parts, can be identified as having a broad range of anti-P. acnes activity...
  27. Pleiotropy, Epistasis, and the Biophysical Adaptation of ssDNA Bacteriophages
    Darin R Rokyta; Fiscal Year: 2013
    ..The proposed experimental system will serve as a model for studying the implications of this type of selection and provide insights into the evolution of infectious diseases. ..
  28. DEFINING THE HUMAN VIROME IN IMMUNOCOMPROMISED CHILDREN
    Gregory A Storch; Fiscal Year: 2013
    ..viruses infecting humans, including viruses causing acute, persistent, or latent infection, but not including bacteriophages or viruses integrated into the human genome without evidence of replicative capacity...
  29. MECHANISTIC STUDIES ON VIRAL ASSEMBLY
    CARLOS CATALANO; Fiscal Year: 1999
    ..Similar mechanisms for DNA packaging have been proposed for all of the double-stranded DNA bacteriophages and may also apply to mammalian viruses such as adenovirus and herpesvirus...
  30. Mechanisms of Sequence-Based Resistance to Viruses and Plasmids in Eubacteria
    Erik J Sontheimer; Fiscal Year: 2010
    ..of many eubacteria and nearly all archaebacteria, have been shown to confer sequence-based immunity against bacteriophages. CRISPR loci are accompanied by a set of cas (CRISPR-associated) genes that are likely to encode protein ..
  31. The Human Vaginal Microbiome and Bacterial Vaginosis
    Bryan White; Fiscal Year: 2010
    ..obtained from non-BV and BV women (3) use a metagenomic approach to understand viral (both human viruses and bacteriophages) diversity and fungal diversity as it relates to BV, and (4) examine the temporal dynamics of the vaginal ..
  32. Mechanistic Studies on a Viral DNA Packaging Machine
    CARLOS CATALANO; Fiscal Year: 2009
    ..in the assembly of many double-stranded DNA viruses, including poxvirus, adenovirus, herpesviruses, and many bacteriophages. Terminase enzymes are common to these viruses and function to package viral DNA into the capsid...
  33. CONFERENCE ON POSITIVE-STRAND RNA VIRUSES
    MARGO BRINTON; Fiscal Year: 1992
    ..families of animal viruses, the majority of the plant viruses, one family of insect viruses and one family of bacteriophages are of this type. Diseases caused by these viruses are of medical, agricultural, and economic significance...
  34. Functional Genomics of Mycobacterium ulcerans Bacteriophages
    STEVEN GAINES CRESAWN; Fiscal Year: 2010
    ..Mycobacterium ulcerans, the causative agent of Buruli ulcer, is susceptible to infection and lysis by bacteriophages. Because bacteriophages have evolved strategies to manipulate the molecular machinery of their hosts, they are ..
  35. Shiga toxin-encoding phage adsorption and toxin release
    MARCIA GOLDBERG; Fiscal Year: 2007
    ..STEC are NIAID Category B Priority pathogens. In many E. coli strains, the stx genes are encoded on lambdoid bacteriophages, and are transmissible from strain to strain by lysogenic conversion...
  36. CONFERENCE ON POSITIVE-STRAND RNA VIRUSES
    MARGO BRINTON; Fiscal Year: 2001
    ..families of animal viruses, eighteen families of plant viruses, one family of insect viruses and one family of bacteriophages. Diseases caused by positive strand RNA viruses are of medical, agricultural and economic significance...
  37. DNA DAMAGE, REPAIR, AND MUTAGENESIS
    IRWIN TESSMAN; Fiscal Year: 1992
    ..The source of damage that will be primarily studied will be UV irradiation. The prokaryotic E. coli with its bacteriophages and the eukaryotic alga Chlorella with its chlorellophages are the organisms that will be studied because they ..
  38. DNA Packaging Motors
    Philip Serwer; Fiscal Year: 2005
    ..The DNA packaging motors to be studied are those of bacteriophages f29, T3 and T7...
  39. HOST RANGE DIVERSITY OF BACTERIOPHAGE FOR Y. PESTIS
    David Martin; Fiscal Year: 2006
    ..As a countermeasure to such a threat, we propose to develop a library of bacteriophages ("phages") specific for Y...
  40. RELEASE AND HOST INACTIVATION OF SSRNA BACTERIOPHAGES
    JEANNE POINDEXTER; Fiscal Year: 1992
    ....
  41. PHAGE-ENCODED FUNCTIONS IN ENTEROHEMORRHAGIC E.COLI
    GAIL CHRISTIE; Fiscal Year: 2001
    ..Genes for both Shiga toxin types in E. coli have been shown to be encoded on lysogenic lambdoid bacteriophages. These Shiga toxin-encoding phages have played an important role in transmitting the stx genes during the ..
  42. NEW ANIMAL MODEL FOR EHEC PATHOGENESIS AND PREVENTION
    Joan Butterton; Fiscal Year: 2004
    ..Strategies have been developed to lysogenize C. rodentium with antibiotic-marked Stx1- and Stx2-expressing bacteriophages as well as to express toxin components from plasmid vectors...
  43. STRUCTURE OF BACTERIAL MEMBRANE PROTEINS
    R Garavito; Fiscal Year: 1992
    ..in creating a defensive barrier against adverse environments, in providing binding sites for the attachment of bacteriophages and bacterial-targeted toxins (e.g...
  44. LIMITATION OF SALMONELLA COLONIZATION WITH PHAGE THERAPY
    John Morris; Fiscal Year: 2000
    We propose to explore the utility of bacteriophages in reducing Salmonella contamination in poultry...
  45. VIBRIO CHOLERAE PATHOGENICITY ISLAND
    David Karaolis; Fiscal Year: 2003
    ..Toxigenic V. cholerae appears to emerge via a multi-step process following the sequential infection of two bacteriophages, initially by VPIphage (whose genome represents a large pathogenicity island) then CTXphage (encoding cholera ..
  46. GENETIC EXCHANGE BY A BACTERIOPHAGE FROM BARTONELLA
    MICHAEL MINNICK; Fiscal Year: 1999
    ..Gene transfer is mediated by bacterial conjugation, transduction via bacteriophages, or natural transformation...
  47. DETERMINANTS OF VIRULENCE IN GROUP A STREPTOCOCCI
    JOSEPH FERRETTI; Fiscal Year: 2002
    ..Gene loss and reacquisition via bacteriophages have been recognized to be key aspects in the evolution and transfer of extracellular bacterial virulence ..
  48. BV ETIOLOGY, NATURAL HISTORY, AND SEXUAL TRANSMISSION
    Maria Wawer; Fiscal Year: 2004
    ..using DNA homology and assessed for H2O2 production) and c) the potential presence of lactobacillus bacteriophages, whose possible role in Lactobacillus depletion will be explored...
  49. NEUTRON DIFFRACTION STUDIES OF VIRUSES
    Lee Makowski; Fiscal Year: 1992
    The structures of the filamentous bacteriophages Pf1 and fd will be studied using coordinated neutron and x-ray fiber diffraction and solid-state NMR spectroscopy...
  50. Mouse Models for EHEC Disease
    Salvatore Cilmi; Fiscal Year: 2005
    ..rodentium with antibiotic-marked Stxl-, Stx2-, and hybrid Stx-expressing bacteriophages. Toxin production, phage induction, and lysogen stability will be examined in vitro...
  51. FASEB CONFERENCE ON VIRAL ASSEMBLY
    Roger Burnett; Fiscal Year: 1992
    ..This led to an interchange and merging of knowledge from research workers in the fields of animal viruses and bacteriophages. Until the advent of the FASEB meetings, these communities had grown apart and so had no common forum...
  52. Acquisition of a 200 kV Cryo-Electron Microscope
    David Chaplin; Fiscal Year: 2005
    ..Luo). Additional studies of assembly of bacteriophages P2 and P4 will be performed by new recruit T. Dokland...
  53. THE BACTERIAL SURFACE AND ITS MULTIFUNCTIONAL DOMAINS
    Manfred Bayer; Fiscal Year: 1990
    ..Furthermore, adhesion areas serve as injection sites for a large variety of bacteriophages. We propose to study a) the ultra-structure and the formation of adhesion sites in relation to cell growth, ..
  54. Structure and Function of Phi29 hexameric pRNA
    Peixuan Guo; Fiscal Year: 2009
    ..feature in the maturation of linear dsDNA viruses, including herpes viruses, adenoviruses, and the ds-DNA bacteriophages, is that the lengthy genome is translocated with remarkable velocity into a limited space within a preformed ..
  55. Novel Integrative Genetic Elements of Mycoplasmas
    Michael Calcutt; Fiscal Year: 2003
    Although horizontal gene transfer by plasmids and bacteriophages has long been known to disseminate key traits among bacterial populations, only recently has the contribution of mobile chromosomal elements been fully realized...
  56. Regulation of restriction-modification genes expression
    Konstantin Severinov; Fiscal Year: 2007
    ..regulation by genetically mobile R-M systems will complement experiments aimed at characterizing molecular mechanisms of host transcription regulation employed by bacteriophages, which are at the core of the parent NIH grant.
  57. ENZYME STEREOCHEMISTRY AND MECHANISM
    DONALD ABERHART; Fiscal Year: 1990
    ..course of deoxycytidylate hydroxymethylase, a methylenetetrahydrofolate-dependent enzyme induced by T-even bacteriophages in E. coli, will be determined...
  58. Bacteriophage-based probiotic preparations and their effect on the gut microflora
    Alexander Sulakvelidze; Fiscal Year: 2007
    ..However, a potentially useful probiotic intervention that has not received much attention is to use bacteriophages to target "problem" bacterial species in the human Gl tract...
  59. STRUCTURAL BASIS OF BACTERIOPHAGE P22 ASSEMBLY
    Wah Chiu; Fiscal Year: 2002
    b>Bacteriophages have long been used as model systems for the study of gene expression, macromolecular assembly, and for cloning vectors in genetic engineering...
  60. Mechanism of Lambda Terminase DNA Processing
    NASIB MALUF; Fiscal Year: 2005
    Double stranded DNA viruses such as adenovirus, the herpesviruses, and several bacteriophages contain a class of enzymes called terminases that function to insert (package) the viral genome into a preformed capsid...
  61. GENE REGULATION BY TRASCRIPTION ANTITERMINATION
    Asis Das; Fiscal Year: 1999
    ..elucidation of fundamental mechanisms that control transcription elongation and termination in bacteria and bacteriophages. Two broad areas of work on elongation control, seeking to achieve several major objectives by combined ..
  62. NMR STUDIES OF BIOLOGICAL SUPRAMOLECULAR STRUCTURES
    STANLEY OPELLA; Fiscal Year: 1992
    ..are integral parts of supramolecular structures and to apply these methods to the study of the filamentous bacteriophages. A direct consequence of the research support by this grant is that solid- state NMR spectroscopy is now on ..
  63. STRUCTURE OF ENGINEERED BACTERIOPHAGE PARTICLES
    Lee Makowski; Fiscal Year: 1992
    ..studies have demonstrated the ease and utility of displaying foreign peptides on the surface of filamentous bacteriophages for applications ranging from screening of epitope libraries to the production of vaccines...