- An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus
The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
Plant J 33:949-56. 2003
- Auxin inhibits endocytosis and promotes its own efflux from cells
Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, 72076 Tubingen, Germany
Nature 435:1251-6. 2005
- Tobacco industry control of menthol in cigarettes and targeting of adolescents and young adults
Jennifer M Kreslake
Tobacco Control Research Program, Division of Public Health Practice, Harvard School of Public Health, 401 Park Dr, Boston, MA 02215, USA harvard edu
Am J Public Health 98:1685-92. 2008
We examined whether tobacco manufacturers manipulate the menthol content of cigarettes in an effort to target adolescents and young adults.
- Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis
John Innes Centre, Colney, Norwich NR4 7UH, Institute of Food Research, Colney, Norwich NR4 7UH, UK
EMBO J 19:6150-61. 2000
..It belongs to a novel group of plant R2R3 MYB proteins involved in transcriptional silencing. The balance between MYB activators and repressors on common target promoters may provide extra flexibility in transcriptional control...
- PIN proteins perform a rate-limiting function in cellular auxin efflux
Institute of Experimental Botany, The Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic
Science 312:914-8. 2006
..Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to ..
- Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10
Richard V Espley
HortResearch, Mt Albert Research Centre, Private Bag 92169, Auckland, New Zealand
Plant J 49:414-27. 2007
..accumulation in both heterologous and homologous systems, generating pigmented patches in transient assays in tobacco leaves and highly pigmented apple plants following stable transformation with constitutively expressed MdMYB10...
- Virus-induced gene silencing in tomato
Department of Molecular, Cellular and Developmental Biology, OML 451, Yale University, PO Box 208104, New Haven, CT 06520 8104, USA
Plant J 31:777-86. 2002
We have previously demonstrated that a tobacco rattle virus (TRV)-based vector can be used in virus-induced gene silencing (VIGS) to study gene function in Nicotiana benthamiana...
- Cytotoxicity evaluation of electronic cigarette vapor extract on cultured mammalian fibroblasts (ClearStream-LIFE): comparison with tobacco cigarette smoke extract
Abich srl, biological and chemical toxicology research laboratory, Verbania VB, Italy
Inhal Toxicol 25:354-61. 2013
..Electronic cigarettes (ECs) are used as alternatives to smoking; however, data on their cytotoxic potential are scarce...
- Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation
Botanical Institute, Zurich Basel Plant Science Centre, University of Basel, Hebelstrasse 1, CH 4056 Basel, Switzerland
Cell 125:749-60. 2006
..These results demonstrate that EFR is the EF-Tu receptor and that plant defense responses induced by PAMPs such as EF-Tu reduce transformation by Agrobacterium...
- Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation
Institut fur Botanik und Botanischer Garten, Molekulare Entwicklungsbiologie der Pflanzen, Universitat Munster, Schlossplatz 4, 48149 Munster, Germany
Plant J 40:428-38. 2004
..the zinc finger protein lesion simulating disease 1 (LSD1) from Arabidopsis as well as the dimer formation of the tobacco 14-3-3 protein T14-3c...
- Root-specific reduction of cytokinin causes enhanced root growth, drought tolerance, and leaf mineral enrichment in Arabidopsis and tobacco
Institute of Biology Applied Genetics, Dahlem Centre of Plant Sciences, Freie Universitat Berlin, D 14195 Berlin, Germany
Plant Cell 22:3905-20. 2010
..We generated transgenic Arabidopsis thaliana and tobacco (Nicotiana tabacum) plants with enhanced root-specific degradation of the hormone cytokinin, a negative regulator ..
- A bacterial virulence protein suppresses host innate immunity to cause plant disease
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
Science 313:220-3. 2006
..HopM1 mediates the destruction of AtMIN7 via the host proteasome. Our results illustrate a strategy by which a bacterial pathogen exploits the host proteasome to subvert host immunity and causes infection in plants...
- EffiCiency and Safety of an eLectronic cigAreTte (ECLAT) as tobacco cigarettes substitute: a prospective 12-month randomized control design study
Centro per la prevenzione e cura del tabagismo, Azienda Ospedaliero Universitaria Policlinico V Emanuele, Universita di Catania, Catania, Italy
PLoS ONE 8:e66317. 2013
..Users report buying them to help quit smoking, to reduce cigarette consumption, to relieve tobacco withdrawal symptoms, and to continue having a 'smoking' experience, but with reduced health risks...
- The TGB1 movement protein of Potato virus X reorganizes actin and endomembranes into the X-body, a viral replication factory
Institute of Molecular Plant Sciences, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
Plant Physiol 158:1359-70. 2012
..Our results indicate that the actin/endomembrane-reorganizing properties of TGB1 function to compartmentalize the viral gene products of PVX infection...
- Caterpillar-induced nocturnal plant volatiles repel conspecific females
C M De Moraes
USDA-ARS, CMAVE, PO Box 14565, Gainesville, Florida 32604, USA
Nature 410:577-80. 2001
..Here we present chemical and behavioural assays showing that tobacco plants (Nicotiana tabacum) release herbivore-induced volatiles during both night and day...
- Recombinant protein production in a variety of Nicotiana hosts: a comparative analysis
Andrew J Conley
Department of Biology, University of Western Ontario, London, ON, Canada
Plant Biotechnol J 9:434-44. 2011
..have been used to produce a wide range of vaccines, antibodies, biopharmaceuticals and industrial enzymes, tobacco has the most established history for the production of recombinant proteins...
- Jasmonate and ppHsystemin regulate key Malonylation steps in the biosynthesis of 17-Hydroxygeranyllinalool Diterpene Glycosides, an abundant and effective direct defense against herbivores in Nicotiana attenuata
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany 07745
Plant Cell 22:273-92. 2010
..HGL-DTGs) that occur in concentrations equivalent to starch (mg/g fresh mass) in aboveground tissues of coyote tobacco (Nicotiana attenuata) and differ in their sugar moieties and malonyl sugar esters (0-2)...
- Stable internal reference genes for normalization of real-time RT-PCR in tobacco (Nicotiana tabacum) during development and abiotic stress
Gregor W Schmidt
Discipline of Genetics, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA, Australia
Mol Genet Genomics 283:233-41. 2010
..b>Tobacco (Nicotiana tabacum) is an important model in studies of plant gene expression, but stable reference genes have ..
- Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure
Institute of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
Plant Biotechnol J 6:392-402. 2008
..beta1,2-xylosyltransferase (XylT) and alpha1,3-fucosyltransferase (FucT) genes in Nicotiana benthamiana, a tobacco-related plant species widely used for recombinant protein expression...
- Metformin prevents tobacco carcinogen--induced lung tumorigenesis
Regan M Memmott
National Cancer Institute Navy Medical Oncology, Room 5101, Building 8, 8901 Wisconsin Avenue, Bethesda, MD 20889, USA
Cancer Prev Res (Phila) 3:1066-76. 2010
Activation of the mammalian target of rapamycin (mTOR) pathway is an important and early event in tobacco carcinogen-induced lung tumorigenesis, and therapies that target mTOR could be effective in the prevention or treatment of lung ..
- A draft genome sequence of Nicotiana benthamiana to enhance molecular plant-microbe biology research
Boyce Thompson Institute for Plant Research, Ithaca, NY, USA
Mol Plant Microbe Interact 25:1523-30. 2012
..The sequence will also be useful for comparative genomics in the Solanaceae family as shown here by the discovery of microsynteny between N. benthamiana and tomato in the region encompassing the Pto and Prf genes...
- Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice
Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA, Deparment of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA and Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68588, USA
Nucleic Acids Res 41:e188. 2013
..systems leading to successful expression of the Cas9/sgRNA system in two dicot plant species, Arabidopsis and tobacco, and two monocot crop species, rice and sorghum...
- 22-Nucleotide RNAs trigger secondary siRNA biogenesis in plants
Ho Ming Chen
Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan
Proc Natl Acad Sci U S A 107:15269-74. 2010
..Our data demonstrate that the function of small RNAs is influenced by size, and that a length of 22 nt facilitates the triggering of secondary siRNA production...
- Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice
R D Hautamaki
Department of Internal Medicine, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, MO 63110, USA
Science 277:2002-4. 1997
..Thus, macrophage elastase is probably sufficient for the development of emphysema that results from chronic inhalation of cigarette smoke...
- Methods to study PAMP-triggered immunity using tomato and Nicotiana benthamiana
Hanh P Nguyen
Boyce Thompson Institute for Plant Research, Ithaca, NY, USA
Mol Plant Microbe Interact 23:991-9. 2010
..These methods will facilitate limited genetic screens and detailed characterization of potential PTI-related genes in model and economically important Solanaceae spp.-pathogen interactions...
- Analysis of potato virus X replicase and TGBp3 subcellular locations
Department of Entomology and Plant Pathology, Noble Research Center, Oklahoma State University, Stillwater, OK 74078, USA
Virology 393:272-85. 2009
..Cerulenin, a drug that inhibits de novo membrane synthesis, also inhibited PVX replication. These combined data indicate that PVX replication relies on ER-derived membrane recruitment and membrane proliferation...
- Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response
Laboratory of Defense in Plant Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi 464 8601, Japan
Plant Cell 23:1153-70. 2011
..Thus, MAPK-mediated phosphorylation of WRKY8 has an important role in the defense response through activation of downstream genes...
- Cellular pathways for viral transport through plasmodesmata
Institut de Biologie Moleculaire des Plantes du CNRS, Universite de Strasbourg, 12 rue du Général Zimmer, 67084, Strasbourg, France
Protoplasma 248:75-99. 2011
..These mechanisms are supported by viral movement proteins and by other virus-encoded factors that interact among themselves and with plant cellular components to facilitate virus movement in a coordinated and regulated fashion...
- Herbivore-associated elicitors: FAC signaling and metabolism
Max Planck Institute for Chemical Ecology, Department of Molecular Ecology, Hans Knoell Str 8, D 07745 Jena, Germany
Trends Plant Sci 16:294-9. 2011
..The interaction between Nicotiana attenuata and the specialist lepidopteran Manduca sexta presents a relevant biological system to understand HAE perception and signal transduction systems in plants...
- Recombinant plant-expressed tumour-associated MUC1 peptide is immunogenic and capable of breaking tolerance in MUC1.Tg mice
The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ, USA
Plant Biotechnol J 9:991-1001. 2011
..The results indicate that a plant-derived human tumour-associated antigen is equivalent to the human antigen in the context of immune recognition...
- Defensive function of herbivore-induced plant volatile emissions in nature
Department of Molecular Ecology, Max-Planck-Institute for Chemical Ecology, Jena 07745, Germany
Science 291:2141-4. 2001
..As a consequence, a plant could reduce the number of herbivores by more than 90% by releasing volatiles. These results confirm that indirect defenses can operate in nature...
- Differential auxin-transporting activities of PIN-FORMED proteins in Arabidopsis root hair cells
Department of Biological Sciences and Genomics and Breeding Institute, Seoul National University, Seoul 151 742, Korea
Plant Physiol 153:1046-61. 2010
..The auxin efflux activity of PIN8, which had not been previously demonstrated, was further confirmed using a tobacco (Nicotiana tabacum) cell assay system...
- Tobacco leaves and roots differ in the expression of proline metabolism-related genes in the course of drought stress and subsequent recovery
Institute of Experimental Botany, v v i, Academy of Sciences of the Czech Republic, Rozvojova 263, 165 02 Prague 6, Lysolaje, Czech Republic
J Plant Physiol 168:1588-97. 2011
..The lack of activation of tobacco PDH genes and incomplete reduction of the P5CS A gene in leaves within 24-h of rehydration may reflect the need ..
- Nonessential plastid-encoded ribosomal proteins in tobacco: a developmental role for plastid translation and implications for reductive genome evolution
Tobias T Fleischmann
Max Planck Institut fur Molekulare Pflanzenphysiologie, D 14476 Potsdam Golm, Germany
Plant Cell 23:3137-55. 2011
..Although plastid translational activity is essential for cell survival in tobacco (Nicotiana tabacum), individual plastid ribosomal proteins can be nonessential...
- A review and assessment of menthol employed as a cigarette flavoring ingredient
J Daniel Heck
Scientific Affairs, AW Spears Research Center, Lorillard Tobacco Company, PO Box 21688, Greensboro, NC 27420 1688, USA
Food Chem Toxicol 48:S1-38. 2010
..critically reviewed and a broad convergence of findings supports a judgment that menthol employed as a cigarette tobacco flavoring ingredient does not meaningfully affect the inherent toxicity of cigarette smoke or the human risks ..
- R2R3-NaMYB8 regulates the accumulation of phenylpropanoid-polyamine conjugates, which are essential for local and systemic defense against insect herbivores in Nicotiana attenuata
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D 07745 Jena, Germany
Plant Physiol 152:1731-47. 2010
..attenuata are consistent with the predictions of the optimal defense theory: plants preferentially protect their most fitness-enhancing and vulnerable parts, young tissues and reproductive organs, to maximize their fitness...
- Insects betray themselves in nature to predators by rapid isomerization of green leaf volatiles
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans Knöll Str 8, DE 07745 Jena, Germany
Science 329:1075-8. 2010
..Hence, attacking herbivores initiate the release of an indirect defense a full day before the attacked plants manufacture their own defensive compounds...
- Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol
Ying Bo Mao
National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, P R China
Nat Biotechnol 25:1307-13. 2007
- Egr-1 regulates autophagy in cigarette smoke-induced chronic obstructive pulmonary disease
Zhi Hua Chen
Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
PLoS ONE 3:e3316. 2008
..Autophagy is a degradative process involving lysosomal turnover of cellular components, though its role in human diseases remains unclear...
- Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis
De Yu Xie
Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
Science 299:396-9. 2003
..Ectopic expression of BAN in tobacco flower petals and Arabidopsis leaves results in loss of anthocyanins and accumulation of CTs.
- Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco
The National Key Facility for Crop Genetic Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
J Exp Bot 60:3781-96. 2009
..Ectopic expression of the GmERF3 gene in transgenic tobacco plants induced the expression of some PR genes and enhanced resistance against infection by Ralstonia ..
- Tobacco smoke carcinogens, DNA damage and p53 mutations in smoking-associated cancers
Gerd P Pfeifer
Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California, CA 91010, USA
Oncogene 21:7435-51. 2002
..p53 mutations are frequent in tobacco-related cancers and the mutation load is often higher in cancers from smokers than from nonsmokers...
- Modulation of transcriptome and metabolome of tobacco by Arabidopsis transcription factor, AtMYB12, leads to insect resistance
National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226 001, India
Plant Physiol 152:2258-68. 2010
..We have expressed an Arabidopsis (Arabidopsis thaliana) transcription factor, AtMYB12, in tobacco (Nicotiana tabacum), which resulted in enhanced expression of genes involved in the phenylpropanoid pathway, ..
- Epigenomic alterations and gene expression profiles in respiratory epithelia exposed to cigarette smoke condensate
Thoracic Oncology Section, Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
Oncogene 29:3650-64. 2010
Limited information is available regarding epigenomic events mediating initiation and progression of tobacco-induced lung cancers...
- An efficient plant viral expression system generating orally immunogenic Norwalk virus-like particles
The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ 85287 5401, USA
Vaccine 26:1846-54. 2008
..We now overcome these constraints with an efficient tobacco mosaic virus (TMV)-derived transient expression system using leaves of Nicotiana benthamiana...
- Determination of tobacco-specific nitrosamines in replacement liquids of electronic cigarettes by liquid chromatography-tandem mass spectrometry
Hyun Ji Kim
Department of Environmental Science, Kongju National University, Kongju 314 701, South Korea
J Chromatogr A 1291:48-55. 2013
A liquid chromatography-tandem mass spectrometric method was described to detect tobacco-specific nitrosamines (TSNAs) in replacement liquids of electronic cigarettes...
- A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Ibaraki, Japan
Plant Cell 6:251-64. 1994
..We analyzed the rd29A promoter in both transgenic Arabidopsis and tobacco and identified a novel cis-acting, dehydration-responsive element (DRE) containing 9 bp, TACCGACAT, that is ..
- Lung carcinogenesis by tobacco smoke
Stephen S Hecht
Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
Int J Cancer 131:2724-32. 2012
..37 million deaths per year...
- British American Tobacco on Facebook: undermining Article 13 of the global World Health Organization Framework Convention on Tobacco Control
A27 School of Public Health, Room 129A, University of Sydney, Sydney, NSW 2006, Australia
Tob Control 19:e1-9. 2010
The World Health Organization Framework Convention on Tobacco Control (WHO FCTC) bans all forms of tobacco advertising, promotion and sponsorship...
- Induction and suppression of RNA silencing by an animal virus
Department of Plant Pathology and Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA
Science 296:1319-21. 2002
..These findings establish RNA silencing as an adaptive antiviral defense in animal cells. B2 also inhibits RNA silencing in transgenic plants, providing evidence for a conserved RNA silencing pathway in the plant and animal kingdoms...
- Silencing of aphid genes by dsRNA feeding from plants
Department of Disease and Stress Biology, The John Innes Centre, Norwich Research Park, Norwich, United Kingdom
PLoS ONE 6:e25709. 2011
- Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum)
Department of Agronomy and James D, Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China
BMC Plant Biol 12:28. 2012
..Over 4,000 miRNAs and several classes of siRNAs have been identified in plants, but in tobacco only computational prediction has been performed and no tobacco-specific miRNA has been experimentally identified...
- A comparative study of the mutagenicity of various types of tobacco products
W S Rickert
Labstat International ULC, 262 Manitou Drive, Kitchener, Ont, Canada N2C 1L3
Regul Toxicol Pharmacol 48:320-30. 2007
Toxicological data are an important aspect of tobacco product characterization...
- Pseudomonas syringae hijacks plant stress chaperone machinery for virulence
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
Proc Natl Acad Sci U S A 107:13177-82. 2010
..Enhanced susceptibility of Hsp70-depleted plants to nonpathogenic strains of P. syringae supports a defense-promoting role for Hsp70...
- Engineering mammalian mucin-type O-glycosylation in plants
Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Flakkebjerg, 4200 Slagelse, Denmark
J Biol Chem 287:11911-23. 2012
..The observed hydroxyproline modifications, however, call for additional future engineering efforts...
- Expression of a wheat MYB gene in transgenic tobacco enhances resistance to Ralstonia solanacearum, and to drought and salt stresses
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Funct Integr Genomics 11:431-43. 2011
..The TaPIMP1 gene was transferred into tobacco (Nicotiana tabacum L.) cultivar W38 by Agrobacterium-mediated transformation...
- A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development
Applied Research Department, Philip Morris International, Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland
Theor Appl Genet 123:219-30. 2011
b>Tobacco (Nicotiana tabacum L.) is a species in the large family of the Solanaceae and is important as an agronomic crop and as a model system in plant biotechnology...
- The less harmful cigarette: a controversial issue. a tribute to Ernst L. Wynder
American Health Foundation, Valhalla, New York 10595, USA
Chem Res Toxicol 14:767-90. 2001
..Laboratory assays with tobacco tar on mouse skin and smoke inhalation experiments with hamsters provided further evidence for this relationship...
- Engineered polyamine catabolism preinduces tolerance of tobacco to bacteria and oomycetes
Panagiotis N Moschou
Department of Biology, University of Crete, Crete, 71409 Heraklion, Greece
Plant Physiol 149:1970-81. 2009
..In wild-type tobacco (Nicotiana tabacum 'Xanthi') plants, infection by the compatible pathogen Pseudomonas syringae pv tabaci resulted ..
- Glycolate oxidase modulates reactive oxygen species-mediated signal transduction during nonhost resistance in Nicotiana benthamiana and Arabidopsis
Clemencia M Rojas
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73402, USA
Plant Cell 24:336-52. 2012
..Here we show that GOX is an alternative source for the production of H(2)O(2) during both gene-for-gene and nonhost resistance responses...
- MYB8 controls inducible phenolamide levels by activating three novel hydroxycinnamoyl-coenzyme A:polyamine transferases in Nicotiana attenuata
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany 07745
Plant Physiol 158:389-407. 2012
..In the native tobacco (Nicotiana attenuata), the accumulation of two major PAs, caffeoylputrescine and dicaffeoylspermidine (DCS), ..
- Quantitative proteomics reveals a dynamic association of proteins to detergent-resistant membranes upon elicitor signaling in tobacco
Institut National de Recherche Agronomique, Unité Mixte de Recherche Plante Microbe Environnement 1088 CNRS 5184 Université de Bourgogne, 17 rue Sully, BP 86510 F 21000 Dijon, France
Mol Cell Proteomics 8:2186-98. 2009
..used (14)N/(15)N metabolic labeling to compare, using a global quantitative proteomics approach, the content of tobacco detergent-resistant membranes extracted from cells treated or not with cryptogein, an elicitor of defense ..
- Cigarette smoke extract induces prolonged endoplasmic reticulum stress and autophagic cell death in human umbilical vein endothelial cells
Division of Experimental Pathophysiology and Immunology, Laboratory of Autoimmunity, Biocenter, Innsbruck Medical University, Innsbruck, Austria
Cardiovasc Res 92:141-8. 2011
- Internalization of flax rust avirulence proteins into flax and tobacco cells can occur in the absence of the pathogen
Division of Plant Science, Research School of Biology, College of Medicine, Biology, and Environment, Australian National University, Canberra, Australian Capital Territory 0200, Australia
Plant Cell 22:2017-32. 2010
..Transient expression of secreted AvrL567 and AvrM proteins fused to cerulean fluorescent protein in tobacco (Nicotiana tabacum) and flax cells resulted in intracellular accumulation of the fusion proteins...
- Cigarettes butts and the case for an environmental policy on hazardous cigarette waste
Thomas E Novotny
Center for Tobacco Control Research and Education University of California San Francisco, San Francisco, CA 94143, USA
Int J Environ Res Public Health 6:1691-705. 2009
..Additional research is needed on the various policy options, including behavioral research on the impact of banning the sale of filtered cigarettes altogether...
- Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants
Icon Genetics, Biozentrum Halle, Weinbergweg 22, D 06120 Halle Saale, Germany
Nat Biotechnol 23:718-23. 2005
..tumefaciens, the high expression yield obtained with viral vectors, and the post-translational capabilities of a plant), does not require genetic modification of plants and is faster than other existing methods...
- Silencing of hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis
Institut de Biologie Moleculaire des Plantes, Unite Propre de Recherche, 2357 du Centre National de la Recherche Scientifique, Universite Louis Pasteur, 67000 Strasbourg, France
Plant Cell 16:1446-65. 2004
..Specific antibodies raised against the recombinant tobacco (Nicotiana tabacum) acyltransferase revealed the accumulation of the enzyme in stem vascular tissues of tobacco, ..
- PIP1 plasma membrane aquaporins in tobacco: from cellular effects to function in plants
Department of Plant Physiology and Biophysics, University of Wurzburg, D 97082 Wurzburg, Germany
Plant Cell 14:869-76. 2002
..The tobacco plasma membrane aquaporin NtAQP1 was used to elucidate this issue...
- Begomovirus-whitefly mutualism is achieved through repression of plant defences by a virus pathogenicity factor
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China
Mol Ecol 21:1294-304. 2012
..As the test plant is an important economic crop, the results also have substantial implications for developing novel strategies for management of crop viruses and the insect vectors associated with them...
- Expression of a Phytophthora sojae necrosis-inducing protein occurs during transition from biotrophy to necrotrophy
Agriculture and Agri Food Canada, 1391 Sandford Street, London, Ontario, Canada N5V 4T3
Plant J 32:361-73. 2002
..This study suggests that the P. sojae necrosis-inducing protein facilitates the colonization of host tissues during the necrotrophic phase of growth...
- Cytokinins mediate resistance against Pseudomonas syringae in tobacco through increased antimicrobial phytoalexin synthesis independent of salicylic acid signaling
Dominik K Grosskinsky
Institute for Plant Sciences, Department of Plant Physiology, University of Graz, 8010 Graz, Austria
Plant Physiol 157:815-30. 2011
..level of bactericidal activities and up-regulated synthesis of the two major antimicrobial phytoalexins in tobacco (Nicotiana tabacum), scopoletin and capsidiol...
- MAPK target networks in Arabidopsis thaliana revealed using functional protein microarrays
Sorina C Popescu
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
Genes Dev 23:80-92. 2009
..Our predicted MKK-MPK phosphorylation network constitutes a valuable resource to understand the function and specificity of MPK signaling systems...
- Pectin methylesterase, a regulator of pollen tube growth
Biology Department, University of Massachusetts, Amherst, Massachusetts 01003, USA
Plant Physiol 138:1334-46. 2005
- Nicotiana benthamiana: its history and future as a model for plant-pathogen interactions
Michael M Goodin
Department of Plant Pathology, University of Kentucky, Lexington 40546, USA
Mol Plant Microbe Interact 21:1015-26. 2008
..Finally, we propose that N. benthamiana is well situated to become a premier plant cell biology model, particularly for the virology community, who as a group were the first to recognize the potential of this unique Australian native...
- The transcriptional innate immune response to flg22. Interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis
The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, United Kingdom
Plant Physiol 135:1113-28. 2004
..Twenty-one tobacco Avr9/Cf-9 rapidly elicited (ACRE) cDNA full-length sequences were used to search for their Arabidopsis orthologs (..
- A viral protein suppresses RNA silencing and binds silencing-generated, 21- to 25-nucleotide double-stranded RNAs
Agricultural Biotechnology Center, Plant Biology Institute, PO Box 411, H 2101, Godollo, Hungary
EMBO J 21:3070-80. 2002
..Moreover, the observation that p19-expressing transgenic plants show altered leaf morphology might indicate that the p19-targeted PTGS pathway is also important in the regulation of plant development...
- Enhancers and core promoter elements are essential for the activity of a cryptic gene activation sequence from tobacco, tCUP
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario
Mol Genet Genomics 265:763-70. 2001
..We have recently isolated such a cryptic sequence from tobacco, tCUP, that can act as a promoter...
- In planta protein sialylation through overexpression of the respective mammalian pathway
Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
J Biol Chem 285:15923-30. 2010
..Besides the biotechnological impact of the achievement, this work may serve as a general model for the manipulation of complex traits into plants...
- Identification and characterization of microRNAs and their target genes in tobacco (Nicotiana tabacum)
Taylor P Frazier
Department of Biology, East Carolina University, Greenville, NC 27858, USA
Planta 232:1289-308. 2010
..Since a list of miRNAs has never been generated for tobacco, we employed genome survey sequence analysis to computationally identify 259 potentially conserved tobacco miRNAs,..
- Indirect activation of a plant nucleotide binding site-leucine-rich repeat protein by a bacterial protease
Department of Biology, Indiana University, Bloomington, IN 47405 7107, USA
Proc Natl Acad Sci U S A 104:2531-6. 2007
..Combined, these data suggest that ATP versus ADP binding functions as a molecular switch that is flipped by cleavage of PBS1...
- Chloroplast-derived enzyme cocktails hydrolyse lignocellulosic biomass and release fermentable sugars
Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32816 2364, USA
Plant Biotechnol J 8:332-50. 2010
..xylan esterase, beta glucosidase and lipase genes from bacteria or fungi were expressed in Escherichia coli or tobacco chloroplasts. A PCR-based method was used to clone genes without introns from Trichoderma reesei genomic DNA...
- The ups and downs of genome size evolution in polyploid species of Nicotiana (Solanaceae)
I J Leitch
Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK
Ann Bot 101:805-14. 2008
..Recent advances in understanding phylogenetic relationships of Nicotiana species and dating polyploid formation enable a temporal dimension to be added to the analysis of genome size evolution in these polyploids...
- Evaluation of the potential effects of ingredients added to cigarettes. Part 2: chemical composition of mainstream smoke
INBIFO Institut für biologische Forschung, Fuggerstr 3, D 51149, Koln, Germany
Food Chem Toxicol 40:93-104. 2002
..caused by the higher transfer rates of the added ingredients to the smoke compared to the transfer from the tobacco part of the filler...
- Tobacco smoking and oral clefts: a meta-analysis
Department of Medicine and Therapeutics, University of Aberdeen, Foresterhill, Scotland
Bull World Health Organ 82:213-8. 2004
..To examine the association between maternal smoking and non-syndromic orofacial clefts in infants...
- A small-cell lung cancer genome with complex signatures of tobacco exposure
Erin D Pleasance
Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK
Nature 463:184-90. 2010
Cancer is driven by mutation. Worldwide, tobacco smoking is the principal lifestyle exposure that causes cancer, exerting carcinogenicity through >60 chemicals that bind and mutate DNA...
- Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Imogen A Sparkes
School of Life Sciences, Oxford Brookes University, Oxford OX3 0BP, UK
Nat Protoc 1:2019-25. 2006
..Transient transformation of tobacco leaf epidermal cells is a relatively fast technique to assess expression of genes of interest...
- Tobacco smoke: involvement of reactive oxygen species and stable free radicals in mechanisms of oxidative damage, carcinogenesis and synergistic effects with other respirable particles
Department of Chemistry, Free Radical Research Group, University of Athens, University Campus Zografou, 15784 Athens, Greece
Int J Environ Res Public Health 6:445-62. 2009
b>Tobacco smoke contains many toxic, carcinogenic and mutagenic chemicals, as well as stable and unstable free radicals and reactive oxygen species (ROS) in the particulate and the gas phase with the potential for biological oxidative ..
- Transcriptional programming and functional interactions within the Phytophthora sojae RXLR effector repertoire
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China
Plant Cell 23:2064-86. 2011
..In support of this hypothesis, misexpression of key immediate-early and early effectors severely reduced the virulence of P. sojae transformants...
- Class VIII myosins are required for plasmodesmatal localization of a closterovirus Hsp70 homolog
Department of Botany and Plant Pathology, Oregon State University, Cordley Hall 2082, Corvallis, OR 97331, USA
J Virol 82:2836-43. 2008
..Interestingly, plasmodesmatal localization of the Tobacco mosaic virus movement protein and Arabidopsis protein RGP2 was not affected by myosin VIII tail overexpression...
- Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic
Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, D 14476 Potsdam Golm, Germany
Plant J 57:436-45. 2009
- Superwobbling facilitates translation with reduced tRNA sets
Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, D 14476 Potsdam Golm, Germany
Nat Struct Mol Biol 15:192-8. 2008
..We also show that the price for superwobbling is a reduced translational efficiency, which explains why most organisms prefer pairs of isoaccepting tRNAs over the superwobbling mechanism...
- Tobacco, a highly efficient green bioreactor for production of therapeutic proteins
Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7
Biotechnol Adv 28:214-21. 2010
..b>Tobacco has been and will continue to be a major crop for molecular farming and offers several practical advantages over ..
- Rapid Akt activation by nicotine and a tobacco carcinogen modulates the phenotype of normal human airway epithelial cells
Kip A West
Cancer Therapeutics Branch, Cell and Cancer Biology Branch, National Cancer Institute, Bethesda, Maryland 20889, USA
J Clin Invest 111:81-90. 2003
b>Tobacco-related diseases such as lung cancer cause over 4.2 million deaths annually, with approximately 400,000 deaths per year occurring in the US...
- The complete nucleotide sequence and multipartite organization of the tobacco mitochondrial genome: comparative analysis of mitochondrial genomes in higher plants
Center for Gene Research, Nagoya University, Chikusa ku, 464 0812 Nagoya, Japan
Mol Genet Genomics 272:603-15. 2005
b>Tobacco is a valuable model system for investigating the origin of mitochondrial DNA (mtDNA) in amphidiploid plants and studying the genetic interaction between mitochondria and chloroplasts in the various functions of the plant cell...
- Plant pathogen recognition mediated by promoter activation of the pepper Bs3 resistance gene
Institute of Biology, Department of Genetics, Martin Luther University Halle Wittenberg, D 06099 Halle Saale, Germany
Science 318:645-8. 2007
..Our data suggest a recognition mechanism in which the Avr protein binds and activates the promoter of the cognate R gene...
- Distribution of fitness and virulence effects caused by single-nucleotide substitutions in Tobacco Etch virus
Instituto de Biología Molecular y Celular de Plantas CSIC UPV, Avenida de los Naranjos s n, 46022 Valencia, Spain
J Virol 81:12979-84. 2007
..Here we used site-directed mutagenesis to create a collection of 66 clones of Tobacco etch potyvirus, each carrying a different, randomly chosen, single-nucleotide substitution...
- Next generation sequencing reveals genome downsizing in allotetraploid Nicotiana tabacum, predominantly through the elimination of paternally derived repetitive DNAs
School of Biological and Chemical Sciences, Queen Mary University of London, London, United Kingdom
Mol Biol Evol 28:2843-54. 2011
..Thus, the three genomes of N. sylvestris, N. tomentosiformis, and N. tabacum have experienced different evolutionary trajectories, with genomes that are dynamic, stable, and downsized, respectively...
- Non-food/feed seeds as biofactories for the high-yield production of recombinant pharmaceuticals
Department of Biotechnology, University of Verona, Verona, Italy
Plant Biotechnol J 9:911-21. 2011
..A vector designed for direct subcloning by Gateway® recombination was developed and tested in Arabidopsis, tobacco and petunia plants for the production of a chimeric form (GAD67/65) of the 65 kDa isoform of glutamic acid ..
- Beyond light and mild: cigarette brand descriptors and perceptions of risk in the International Tobacco Control (ITC) Four Country Survey
Department of Health Studies and Gerontology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada
Addiction 106:1166-75. 2011
..To examine perceptions of risk related to type of cigarette brand...
- Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway
Unité Mixte de Recherche 619, Physiologie et Biotechnologie Végétales, Universite Bordeaux 1, Universite Bordeaux 2, Institut National de la Recherche Agonomique, Centre de Recherche de Bordeaux, 33883 Villenave d Ornon, France
Plant Physiol 140:499-511. 2006
..Overexpression of VvMYB5a in tobacco (Nicotiana tabacum) affects the expression of structural genes controlling the synthesis of phenylpropanoid and ..
- Cloning and molecular characterization of a mitogen-activated protein kinase gene from Poncirus trifoliata whose ectopic expression confers dehydration/drought tolerance in transgenic tobacco
Xiao San Huang
Key Laboratory of Horticultural Plant Biology of the Ministry of Education, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
J Exp Bot 62:5191-206. 2011
..Transgenic overexpression of PtrMAPK in tobacco confers dehydration and drought tolerance...