genetically modified animals

Summary

Summary: ANIMALS whose GENOME has been altered by GENETIC ENGINEERING, or their offspring.

Top Publications

  1. ncbi A Drosophila model of Parkinson's disease
    M B Feany
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 404:394-8. 2000
  2. ncbi Entrainment of the circadian clock in the liver by feeding
    K A Stokkan
    National Science Foundation Center for Biological Timing and Department of Biology, University of Virginia, P O Box 400328, Charlottesville, VA 22904 4328, USA
    Science 291:490-3. 2001
  3. ncbi Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
    L Timmons
    Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
    Gene 263:103-12. 2001
  4. ncbi Tissue-specific activities of C. elegans DAF-16 in the regulation of lifespan
    Nataliya Libina
    Department of Biochemistry and Biophysics, Mission Bay Genentech Hall, 600 16th Street, Room S312D, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell 115:489-502. 2003
  5. ncbi Spatiotemporal rescue of memory dysfunction in Drosophila
    Sean E McGuire
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 302:1765-8. 2003
  6. ncbi In vivo imaging of embryonic vascular development using transgenic zebrafish
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD NIH, Building 6B, 6 Center Drive, Bethesda, MD 20892, USA
    Dev Biol 248:307-18. 2002
  7. pmc Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31
    Amy C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
    Genetics 166:1775-82. 2004
  8. ncbi Effective targeted gene 'knockdown' in zebrafish
    A Nasevicius
    Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Department of Genetics, Cell Biology and Development, Minneapolis, Minnesota, USA
    Nat Genet 26:216-20. 2000
  9. ncbi Ablation of insulin-producing neurons in flies: growth and diabetic phenotypes
    Eric J Rulifson
    Department of Developmental Biology, Beckman Center B300, Stanford University, Stanford, CA 94305 5329, USA
    Science 296:1118-20. 2002
  10. ncbi Animal models of human disease: zebrafish swim into view
    Graham J Lieschke
    Cancer and Haematology Division, Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, 3050, Australia
    Nat Rev Genet 8:353-67. 2007

Research Grants

  1. Targeted delivery of butyrate to the colon in mice
    SHARON FLEMING; Fiscal Year: 2006
  2. Transgenesis and Xenotransplantation
    Jorge A Piedrahita; Fiscal Year: 2013
  3. Targeted genome modification of guinea pig and sheep using engineered zinc finger
    Hilda Holcombe; Fiscal Year: 2011
  4. Targeted genome modification of guinea pig and sheep using engineered zinc finger
    Hilda Holcombe; Fiscal Year: 2012
  5. The Role of AP-1 in the Gonadotrope
    Djurdjica Coss; Fiscal Year: 2013
  6. Regulation and Function of Tissue Kallikrein
    Julie Chao; Fiscal Year: 2012
  7. Animal Models and Drug Discovery
    Garret A Fitzgerald; Fiscal Year: 2011
  8. Cytokine regulation of memory CD8 T cell responses
    Kimberly D Klonowski; Fiscal Year: 2013
  9. Striatin, Aldosterone and Hypertension
    Gordon H Williams; Fiscal Year: 2013
  10. ETHANOL SENSITIVITY OF NATIVE AND CLONED NMDA RECEPTORS
    John J Woodward; Fiscal Year: 2013

Detail Information

Publications335 found, 100 shown here

  1. ncbi A Drosophila model of Parkinson's disease
    M B Feany
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 404:394-8. 2000
    ..Our Drosophila model thus recapitulates the essential features of the human disorder, and makes possible a powerful genetic approach to Parkinson's disease...
  2. ncbi Entrainment of the circadian clock in the liver by feeding
    K A Stokkan
    National Science Foundation Center for Biological Timing and Department of Biology, University of Virginia, P O Box 400328, Charlottesville, VA 22904 4328, USA
    Science 291:490-3. 2001
    ..They also raise the possibility that peripheral circadian oscillators like those in the liver may be coupled to the SCN primarily through rhythmic behavior, such as feeding...
  3. ncbi Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
    L Timmons
    Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
    Gene 263:103-12. 2001
    ....
  4. ncbi Tissue-specific activities of C. elegans DAF-16 in the regulation of lifespan
    Nataliya Libina
    Department of Biochemistry and Biophysics, Mission Bay Genentech Hall, 600 16th Street, Room S312D, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell 115:489-502. 2003
    ..We suggest that this network of tissue interactions and feedback regulation allows the tissues to equilibrate and fine-tune their expression of downstream genes, which, in turn, coordinates their rates of aging within the animal...
  5. ncbi Spatiotemporal rescue of memory dysfunction in Drosophila
    Sean E McGuire
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 302:1765-8. 2003
    ..The TARGET system offers general utility in simultaneously addressing issues of when and where gene products are required...
  6. ncbi In vivo imaging of embryonic vascular development using transgenic zebrafish
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD NIH, Building 6B, 6 Center Drive, Bethesda, MD 20892, USA
    Dev Biol 248:307-18. 2002
    ....
  7. pmc Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31
    Amy C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
    Genetics 166:1775-82. 2004
    ..These experiments demonstrate the potential for precise genetic engineering of the Drosophila genome with the phiC31 integrase system and will likely benefit research in Drosophila and other insects...
  8. ncbi Effective targeted gene 'knockdown' in zebrafish
    A Nasevicius
    Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Department of Genetics, Cell Biology and Development, Minneapolis, Minnesota, USA
    Nat Genet 26:216-20. 2000
    ..Conserved vertebrate processes and diseases are now amenable to a systematic, in vivo, reverse-genetic paradigm using zebrafish embryos...
  9. ncbi Ablation of insulin-producing neurons in flies: growth and diabetic phenotypes
    Eric J Rulifson
    Department of Developmental Biology, Beckman Center B300, Stanford University, Stanford, CA 94305 5329, USA
    Science 296:1118-20. 2002
    ..Interestingly, the phenotype of flies lacking IPCs includes certain features of diabetes mellitus...
  10. ncbi Animal models of human disease: zebrafish swim into view
    Graham J Lieschke
    Cancer and Haematology Division, Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, 3050, Australia
    Nat Rev Genet 8:353-67. 2007
    ..This Review surveys the achievements and potential of zebrafish for modelling human diseases and for drug discovery and development...
  11. ncbi An evolutionarily conserved function of the Drosophila insulin receptor and insulin-like peptides in growth control
    W Brogiolo
    Zoologisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057, Zurich, Switzerland
    Curr Biol 11:213-21. 2001
    ....
  12. ncbi A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish
    Koichi Kawakami
    Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Dev Cell 7:133-44. 2004
    ..We propose our gene trap approach should facilitate studies of vertebrate development and organogenesis...
  13. ncbi A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
    Georg Dietzl
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, A 1030 Vienna, Austria
    Nature 448:151-6. 2007
    ..Our transgenic RNAi library thus opens up the prospect of systematically analysing gene functions in any tissue and at any stage of the Drosophila lifespan...
  14. pmc Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish
    Kazuhide Asakawa
    Division of Molecular and Developmental Biology, National Institute of Genetics, and Department of Genetics, Graduate University for Advanced Studies, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Proc Natl Acad Sci U S A 105:1255-60. 2008
    ..These studies illustrate that our Gal4FF gene trap and enhancer trap methods should be an important resource for genetic analysis of neuronal functions and behavior in vertebrates...
  15. ncbi Reiterative use of the EGF receptor triggers differentiation of all cell types in the Drosophila eye
    M Freeman
    MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
    Cell 87:651-60. 1996
    ..These results broaden our view of eye development to include the whole ommatidium and suggest that reiterative activation of DER is critical for triggering the differentiation of all cell types...
  16. ncbi Histone deacetylase inhibitors arrest polyglutamine-dependent neurodegeneration in Drosophila
    J S Steffan
    Department of Psychiatry and Human Behavior, Gillespie 2121, University of California, Irvine, California 92697, USA
    Nature 413:739-43. 2001
    ..These findings raise the possibility that therapy with HDAC inhibitors may slow or prevent the progressive neurodegeneration seen in Huntington's disease and other polyglutamine-repeat diseases, even after the onset of symptoms...
  17. ncbi GAL4 system in Drosophila: a fly geneticist's Swiss army knife
    Joseph B Duffy
    Department of Biology, Indiana University, 1001 E 3rd Street, Bloomington, IN 47405, USA
    Genesis 34:1-15. 2002
  18. pmc Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system
    M Maduro
    Department of Biological Sciences, University of Alberta, Edmonton, Canada
    Genetics 141:977-88. 1995
    ..Expression of an unc-119/lacZ fusion in transgenic animals is seen in many neurons, suggesting that the unc-119 mutant phenotype is due to a defect in the nervous system...
  19. ncbi Germ-line transmission of a myocardium-specific GFP transgene reveals critical regulatory elements in the cardiac myosin light chain 2 promoter of zebrafish
    Chiu Ju Huang
    Institute of Molecular and Cell Biology, National Taiwan University, Taipei, Taiwan
    Dev Dyn 228:30-40. 2003
    ....
  20. ncbi Sirtuin 2 inhibitors rescue alpha-synuclein-mediated toxicity in models of Parkinson's disease
    Tiago Fleming Outeiro
    Alzheimer s Research Unit, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129, USA
    Science 317:516-9. 2007
    ..Furthermore, the inhibitors protected against dopaminergic cell death both in vitro and in a Drosophila model of Parkinson's disease. The results suggest a link between neurodegeneration and aging...
  21. pmc Alpha-synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson's models
    Antony A Cooper
    School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
    Science 313:324-8. 2006
    ..Thus, synucleinopathies may result from disruptions in basic cellular functions that interface with the unique biology of particular neurons to make them especially vulnerable...
  22. ncbi Rest in Drosophila is a sleep-like state
    J C Hendricks
    Center for Sleep and Respiratory Neurobiology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    Neuron 25:129-38. 2000
    ..Finally, normal homeostatic regulation depends on the timeless but not the period central clock gene. Understanding the molecular features of Drosophila rest should shed new light on the mechanisms and function of sleep...
  23. pmc Dissecting the pathological effects of human Abeta40 and Abeta42 in Drosophila: a potential model for Alzheimer's disease
    Koichi Iijima
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 101:6623-8. 2004
    ..Moreover, Drosophila may serve as a model for facilitating the understanding of molecular mechanisms underlying Abeta toxicity and the discovery of novel therapeutic targets for AD...
  24. ncbi Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning
    Liangxue Lai
    Department of Animal Science, University of Missouri, Columbia, MO 65211, USA
    Science 295:1089-92. 2002
    ..These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes...
  25. pmc Late-acting dominant lethal genetic systems and mosquito control
    Hoang Kim Phuc
    Department of Zoology, University of Oxford, South Parks Road, Oxford, UK
    BMC Biol 5:11. 2007
    ..Additionally, vector populations with strong density-dependent effects will tend to be resistant to SIT-based control as the population-reducing effect of induced sterility will tend to be offset by reduced density-dependent mortality...
  26. ncbi Mitochondrial dysfunction in Drosophila PINK1 mutants is complemented by parkin
    Jeehye Park
    Nature 441:1157-61. 2006
    ..Taken together, our genetic evidence clearly establishes that Parkin and PINK1 act in a common pathway in maintaining mitochondrial integrity and function in both muscles and dopaminergic neurons...
  27. ncbi Cellular and molecular analyses of vascular tube and lumen formation in zebrafish
    Suk Won Jin
    Department of Biochemistry and Biophysics and Cardiovascular Research Institute, Program in Developmental Biology, University of California San Francisco, 1550 Fourth Street, San Francisco, CA 94143, USA
    Development 132:5199-209. 2005
    ..These studies provide the tools and a cellular framework for the investigation of mutations affecting vasculogenesis in zebrafish...
  28. ncbi CRY, a Drosophila clock and light-regulated cryptochrome, is a major contributor to circadian rhythm resetting and photosensitivity
    P Emery
    Department of Biology, Brandeis University Waltham, Massachusetts 02454, USA
    Cell 95:669-79. 1998
    ..Taken together with the data in the accompanying paper, we propose that CRY is a major Drosophila photoreceptor dedicated to the resetting of circadian rhythms...
  29. pmc Distinct requirements for somatic and germline expression of a generally expressed Caernorhabditis elegans gene
    W G Kelly
    Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Genetics 146:227-38. 1997
    ..The resulting transgenes show robust expression in both germline and soma. Our results suggest the possibility of concerted mechanisms for silencing unwanted germiline expression of repetitive sequences...
  30. ncbi Formation of the digestive system in zebrafish. I. Liver morphogenesis
    Holly A Field
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, University of California, San Francisco, San Francisco, CA 94143, USA
    Dev Biol 253:279-90. 2003
    ....
  31. pmc Transactivation from Gal4-VP16 transgenic insertions for tissue-specific cell labeling and ablation in zebrafish
    Jon M Davison
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Dev Biol 304:811-24. 2007
    ....
  32. ncbi Spatiotemporal gene expression targeting with the TARGET and gene-switch systems in Drosophila
    Sean E McGuire
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Sci STKE 2004:pl6. 2004
    ..In this protocol, we provide guidelines for the use of these two novel systems, which should have general utility in studying Drosophila biology and in using the fly as a model for human disease...
  33. pmc Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans
    R F Ketting
    The Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, Utrecht, The Netherlands
    Genes Dev 15:2654-9. 2001
    ..dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41)...
  34. pmc Foxn4 directly regulates tbx2b expression and atrioventricular canal formation
    Neil C Chi
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, University of California, San Francisco, San Francisco, California 94158, USA
    Genes Dev 22:734-9. 2008
    ..sli/foxn4 is expressed in the AV canal, and its encoded product binds to a highly conserved tbx2 enhancer domain that contains Foxn4- and T-box-binding sites, both necessary to regulate tbx2b expression in the AV canal...
  35. ncbi Remote control of neuronal activity with a light-gated glutamate receptor
    Stephanie Szobota
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Neuron 54:535-45. 2007
    ..Our studies show that LiGluR provides robust control over neuronal activity, enabling the dissection and manipulation of neural circuitry in vivo...
  36. ncbi Resolution of inflammation by retrograde chemotaxis of neutrophils in transgenic zebrafish
    Jonathan R Mathias
    University of Wisconsin Madison, 2715 Medical Sciences Center, 1300 University Ave, Madison, WI 53706, USA
    J Leukoc Biol 80:1281-8. 2006
    ..The zMPO:GFP zebrafish provides unique insight into the mechanisms of neutrophil-mediated inflammation and thereby offers opportunities to identify new regulators of the inflammatory response in vivo...
  37. ncbi I-SceI meganuclease mediates highly efficient transgenesis in fish
    Violette Thermes
    INRA Junior Group, UPR2197, Institut de Neurobiologie A Fessard, CNRS, Avenue de la Terrasse, 91 198 Gif sur Yvette, France
    Mech Dev 118:91-8. 2002
    ..Meganuclease co-injection thus provides a simple and highly efficient tool to improve transgenesis by microinjection...
  38. ncbi PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C. elegans
    Oliver Hobert
    Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA
    Biotechniques 32:728-30. 2002
  39. ncbi Myc-induced T cell leukemia in transgenic zebrafish
    David M Langenau
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 299:887-90. 2003
    ..This transgenic model provides a platform for drug screens and for genetic screens aimed at identifying mutations that suppress or enhance c-myc- induced carcinogenesis...
  40. ncbi Development of the zebrafish lymphatic system requires VEGFC signaling
    Axel M Küchler
    Exelixis, Inc, 170 Harbor Way, South San Francisco, California 94083, USA
    Curr Biol 16:1244-8. 2006
    ....
  41. ncbi Taste perception and coding in Drosophila
    Natasha Thorne
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, 252 CARL Building Research Drive, Durham, NC 27710 USA
    Curr Biol 14:1065-79. 2004
    ..In Drosophila, a family of gustatory receptors (GRs) expressed in taste neurons is thought to mediate the recognition of sugars and bitter compounds, thereby controlling feeding behavior...
  42. ncbi wishful thinking encodes a BMP type II receptor that regulates synaptic growth in Drosophila
    Hermann Aberle
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Neuron 33:545-58. 2002
    ..The NMJ phenotype is specifically rescued by transgenic expression of Wit only in motoneurons. Thus, Wit appears to function as a presynaptic receptor that regulates synaptic size at the Drosophila NMJ...
  43. ncbi Conditional targeted cell ablation in zebrafish: a new tool for regeneration studies
    Silvia Curado
    Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California at San Francisco, San Francisco, California 94158 2324, USA
    Dev Dyn 236:1025-35. 2007
    ....
  44. pmc An AMPK-FOXO pathway mediates longevity induced by a novel method of dietary restriction in C. elegans
    Eric L Greer
    Department of Genetics, Cancer Biology Program, Stanford University, Stanford, California 94305, USA
    Curr Biol 17:1646-56. 2007
    ..AMP-activated protein kinase (AMPK) is activated by a decrease in energy levels, raising the possibility that AMPK might mediate lifespan extension by DR...
  45. ncbi A sleep-promoting role for the Drosophila serotonin receptor 1A
    Quan Yuan
    Howard Hughes Medical Institute, Center for Sleep and Respiratory Neurobiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Curr Biol 16:1051-62. 2006
    ..Serotonin is an important neurotransmitter known to affect many behaviors, but its role in sleep remains controversial...
  46. ncbi A transgenic Lef1/beta-catenin-dependent reporter is expressed in spatially restricted domains throughout zebrafish development
    Richard I Dorsky
    Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Dev Biol 241:229-37. 2002
    ..We conclude that our TOP-dGFP reporter line faithfully illustrates domains of beta-catenin activity and enables the identification of responsive cell populations...
  47. ncbi Genetic and cellular analyses of zebrafish atrioventricular cushion and valve development
    Dimitris Beis
    Department of Biochemistry and Biophysics and Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
    Development 132:4193-204. 2005
    ..This collection of mutants provides a unique set of tools to further our understanding of the genetic basis of cell behavior and differentiation during AV valve development...
  48. ncbi Expanded polyglutamine protein forms nuclear inclusions and causes neural degeneration in Drosophila
    J M Warrick
    Department of Biology, University of Pennsylvania and University of Pennsylvania Medical School, Philadelphia 19104, USA
    Cell 93:939-49. 1998
    ..Our results demonstrate that cellular mechanisms of human glutamine-repeat disease are conserved in invertebrates. This fly model will aid in identifying additional factors that modulate neurodegeneration...
  49. pmc UNC-6/Netrin induces neuronal asymmetry and defines the site of axon formation
    Carolyn E Adler
    Howard Hughes Medical Institute, Laboratory of Neural Circuits and Behavior, Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nat Neurosci 9:511-8. 2006
    ..The coupling of a directional netrin cue to sustained asymmetric growth via PI3K signaling is reminiscent of polarization in chemotaxing cells...
  50. pmc Temporal analysis of meiotic DNA double-strand break formation and repair in Drosophila females
    S Mehrotra
    Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
    PLoS Genet 2:e200. 2006
    ..From a comparison of the frequency of gamma-His2Av foci and crossovers, it appears that Drosophila females have only a weak mechanism to ensure a crossover in the presence of a low number of DSBs...
  51. ncbi Molecular pathways of neurodegeneration in Parkinson's disease
    Ted M Dawson
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Science 302:819-22. 2003
    ..Strategies aimed at restoring complex I activity, reducing oxidative stress and alpha-synuclein aggregation, and enhancing protein degradation may hold particular promise as powerful neuroprotective agents in the treatment of PD...
  52. ncbi Resetting central and peripheral circadian oscillators in transgenic rats
    S Yamazaki
    NSF Center for Biological Timing and Department of Biology, University of Virginia, Charlottesville, VA 22903-2477, USA
    Science 288:682-5. 2000
    ....
  53. ncbi A versatile vector set for animal transgenesis
    C Horn
    Lehrstuhl fur Genetik, Universitat Bayreuth, Universitätsstrasse 30 NWI, 95447 Bayreuth, Germany
    Dev Genes Evol 210:630-7. 2000
    ..Using this system we demonstrate that our vectors transpose in both Drosophila melanogaster and Drosophila virilis...
  54. ncbi Transgenesis and gene trap methods in zebrafish by using the Tol2 transposable element
    Koichi Kawakami
    Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Methods Cell Biol 77:201-22. 2004
  55. ncbi A transgenic zebrafish model of neutrophilic inflammation
    Stephen A Renshaw
    MRC Centre for Developmental and Biomedical Genetics, Acadamic Unit of Respiratory Medicine, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    Blood 108:3976-8. 2006
    ..fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model...
  56. ncbi Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system
    M Schaefer
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Cell 107:183-94. 2001
    ..Thus, asymmetric activation of heterotrimeric G proteins by a receptor-independent mechanism may orient asymmetric cell divisions in different cell types...
  57. ncbi Resident aliens: the Tc1/mariner superfamily of transposable elements
    R H Plasterk
    Division of Molecular Biology, Netherlands Cancer Institute and Center for Biomedical Genetics, Division of Molecular Biology, Plesmanlaan 121, Amsterdam 1066CX, The Netherlands
    Trends Genet 15:326-32. 1999
    ....
  58. pmc Identification of an outer segment targeting signal in the COOH terminus of rhodopsin using transgenic Xenopus laevis
    B M Tam
    Program in Neuroscience, Department of Pharmacology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Cell Biol 151:1369-80. 2000
    ..The targeting and transport of endogenous wild-type rhodopsin was unaffected by the presence of mislocalized GFP fusion proteins...
  59. pmc Creation of low-copy integrated transgenic lines in Caenorhabditis elegans
    V Praitis
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    Genetics 157:1217-26. 2001
    ..In addition, we find that low-copy integrated lines can also be used to express transgenes in the C. elegans germline, where conventional extrachromosomal arrays typically fail to express due to germline silencing...
  60. ncbi Diverse functions of neuropeptide Y revealed using genetically modified animals
    A Thorsell
    1Department of Neuropharmacology, The Scripps Research Institute, CVN 15, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Neuropeptides 36:182-93. 2002
    ..approaches, such as lack of selectivity of receptor antagonists, can be elegantly circumvented using genetically modified animals. For NPY, mice lacking NPY, the Y1, the Y2 or the Y5 receptors have been generated...
  61. ncbi Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants
    David Traver
    Children s Hospital Boston and the Howard Hughes Medical Institute, 320 Longwood Avenue, Enders 720, Boston, Massachusetts 02115, USA
    Nat Immunol 4:1238-46. 2003
    ..Direct visualization of fluorescent donor cells in embryonic recipients allows engraftment and homing events to be imaged in real time. These results provide a cellular context in which to study the genetics of hematopoiesis...
  62. ncbi Feed-back mechanisms affecting Notch activation at the dorsoventral boundary in the Drosophila wing
    J F de Celis
    Department of Genetics, University of Cambridge, UK
    Development 124:3241-51. 1997
    ..Thus the combined effects of Notch and its target genes cut and wingless regulate the expression of Notch ligands which restrict Notch activity to the dorsoventral boundary...
  63. ncbi A direct role for Sox10 in specification of neural crest-derived sensory neurons
    Thomas J Carney
    Centre for Regenerative Medicine, Developmental Biology Programme, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Development 133:4619-30. 2006
    ..In conjunction with previous findings, these data establish the generality of our model of Sox10 function in NC fate specification...
  64. ncbi Amyloid-beta-induced pathological behaviors are suppressed by Ginkgo biloba extract EGb 761 and ginkgolides in transgenic Caenorhabditis elegans
    Yanjue Wu
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, Maryland 21201, USA
    J Neurosci 26:13102-13. 2006
    ....
  65. pmc High-resolution ChIP-chip analysis reveals that the Drosophila MSL complex selectively identifies active genes on the male X chromosome
    Artyom A Alekseyenko
    Howard Hughes Medical Institute, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Genes Dev 20:848-57. 2006
    ..Distinguishing expressed genes from the bulk of the genome is likely to be an important function common to many chromatin organizing and modifying activities...
  66. ncbi Activation of EGFR and ERK by rhomboid signaling regulates the consolidation and maintenance of sleep in Drosophila
    Krisztina Foltenyi
    Department of Biology, University of California San Diego, 9500 Gilman Dr, La Jolla, California 92093, USA
    Nat Neurosci 10:1160-7. 2007
    ..These results suggest that sleep and its regulation by EGFR signaling may be ancestral to insects and mammals...
  67. pmc A sensory feedback circuit coordinates muscle activity in Drosophila
    Cynthia L Hughes
    Molecular Neurobiology Laboratory, The Salk Institute, 10010 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Mol Cell Neurosci 35:383-96. 2007
    ....
  68. pmc The sex peptide of Drosophila melanogaster: female post-mating responses analyzed by using RNA interference
    Tracey Chapman
    Department of Biology, Darwin Building, and Wolfson Institute for Biomedical Research, Cruciform Building, University College London, Gower Street, London WC1E 6BT, UK
    Proc Natl Acad Sci U S A 100:9923-8. 2003
    ..The prolonged "sperm effect" on female receptivity and egg production is therefore entirely attributable to SP, but sperm are necessary for its occurrence...
  69. ncbi Moesin functions antagonistically to the Rho pathway to maintain epithelial integrity
    Olga Speck
    DCMB Group, Department of Biology, Duke University, Box 91000, Durham, North Carolina 27708, USA
    Nature 421:83-7. 2003
    ..Furthermore, our results show that there is negative feedback between ERM activation and activity of the Rho pathway...
  70. ncbi The cat-1 gene of Caenorhabditis elegans encodes a vesicular monoamine transporter required for specific monoamine-dependent behaviors
    J S Duerr
    Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    J Neurosci 19:72-84. 1999
    ..elegans VMAT constructs and also (at least partially) by human VMAT1 or VMAT2 transgenes. It therefore appears that the function of amine neurotransmitters can be completely dependent on their loading into synaptic vesicles...
  71. ncbi Calcium dysregulation in Alzheimer's disease: recent advances gained from genetically modified animals
    Ian F Smith
    Department of Neurobiology and Behavior, University of California, 1109 Gillespie Neuroscience Building, Irvine CA 92697 4545, USA
    Cell Calcium 38:427-37. 2005
    ..The cause of synaptic dysfunction is unknown but it is likely that amyloid-beta and its ability to disrupt intracellular calcium homeostasis plays a key role in this process...
  72. pmc Genetic modifiers of tauopathy in Drosophila
    Joshua M Shulman
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 165:1233-42. 2003
    ..Our results demonstrate that kinases and phosphatases control Tau-induced neurodegeneration and have important implications for the development of therapies in Alzheimer's disease and related disorders...
  73. ncbi Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors
    Carlos Lois
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 295:868-72. 2002
    ..We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species...
  74. ncbi In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio)
    Guor Mour Her
    Laboratory of Marine Molecular Biology and Biotechnology, Institute of Zoology, Academia Sinica, Nankang, Taipei 115, Taiwan
    FEBS Lett 538:125-33. 2003
    ..Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development...
  75. ncbi C. elegans daf-6 encodes a patched-related protein required for lumen formation
    Elliot A Perens
    Laboratory of Developmental Genetics, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Dev Cell 8:893-906. 2005
    ..We propose that amphid lumen morphogenesis is coordinated by neuron-derived cues and a DAF-6/CHE-14 system that regulates vesicle dynamics during tubulogenesis...
  76. pmc A dual leucine kinase-dependent axon self-destruction program promotes Wallerian degeneration
    Bradley R Miller
    Department of Developmental Biology, Hope Center for Neurological Disorders, Washington University School of Medicine, St Louis, Missouri, USA
    Nat Neurosci 12:387-9. 2009
    ..This pathway also promoted degeneration after chemotherapy exposure and may be a component of a general axon self-destruction program...
  77. ncbi Mammalian tumor xenografts induce neovascularization in zebrafish embryos
    Stefania Nicoli
    Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, Brescia, Italy
    Cancer Res 67:2927-31. 2007
    ..These data show that the zebrafish/tumor xenograft model represents a novel tool for investigating the neovascularization process exploitable for drug discovery and gene targeting in tumor angiogenesis...
  78. ncbi Chaperone suppression of alpha-synuclein toxicity in a Drosophila model for Parkinson's disease
    Pavan K Auluck
    Department of Neuroscience, University of Pennsylvania and University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 295:865-8. 2002
    ..Furthermore, Lewy bodies in human postmortem tissue immunostained for molecular chaperones, also suggesting that chaperones may play a role in Parkinson's disease progression...
  79. ncbi A 3'UTR pumilio-binding element directs translational activation in olfactory sensory neurons
    Julia A Kaye
    Cellular and Developmental Biology Program, 1 Shields Drive, University of California, Davis, Davis, CA 95616, USA
    Neuron 61:57-70. 2009
    ....
  80. ncbi Green fluorescent protein expression in germ-line transmitted transgenic zebrafish under a stratified epithelial promoter from keratin8
    Zhiyuan Gong
    Department of Biological Sciences, National University of Singapore, Singapore
    Dev Dyn 223:204-15. 2002
    ....
  81. pmc Adenomatous polyposis coli-deficient zebrafish are susceptible to digestive tract neoplasia
    Anna Pavlina G Haramis
    Hubrecht Laboratorium, NIOB KNAW, Center for Biomedical Genetics, CT Utrecht, The Netherlands
    EMBO Rep 7:444-9. 2006
    ..Treatment with the chemical carcinogen 7,12-dimethylbenz[a]anthracene accelerates the induction of these lesions. These observations establish apc-mutant zebrafish as a bona fide model for the study of digestive tract cancer...
  82. ncbi Slit coordinates cardiac morphogenesis in Drosophila
    Allison MacMullin
    Department of Biology, McMaster University, LSB 429, 1280 Main St W, Hamilton, ON, Canada L8S 4K1
    Dev Biol 293:154-64. 2006
    ..This indicates that Slit participates in adhesion or adhesion signaling during heart development...
  83. ncbi On the role of RNA amplification in dsRNA-triggered gene silencing
    T Sijen
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Cell 107:465-76. 2001
    ..This amplification mechanism substantially augments the potency of RNAi-based surveillance, while ensuring that the RNAi machinery will focus on expressed mRNAs...
  84. ncbi T-cell responses during pig-to-primate xenotransplantation
    Eduardo Davila
    Department of Pediatrics, Louisiana State University Health Science Center, Stanley S Scott Cancer Center, New Orleans, LA, USA
    Xenotransplantation 13:31-40. 2006
    ..To determine the potential role of T lymphocytes in Xenograft (Xg) rejection, we transplanted transgenic hCD46 porcine hearts heterotopically into baboon recipients...
  85. pmc Neuronal signaling modulates protein homeostasis in Caenorhabditis elegans post-synaptic muscle cells
    Susana M Garcia
    Department of Biochemistry, Molecular Biology and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, Illinois 60208, USA
    Genes Dev 21:3006-16. 2007
    ..These results demonstrate the importance of intercellular communication in intracellular homeostasis...
  86. ncbi Transgenic animal bioreactors
    L M Houdebine
    Unité de biologie du développement et biotechnologie, Institut National de la Recherche Agronomique, Jouy en Josas, France
    Transgenic Res 9:305-20. 2000
    ....
  87. pmc Tools for neuroanatomy and neurogenetics in Drosophila
    Barret D Pfeiffer
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn VA 20147, USA
    Proc Natl Acad Sci U S A 105:9715-20. 2008
    ..We expect that these lines will be valuable tools for neuroanatomy as well as for the elucidation of neuronal circuits and information flow in the fly brain...
  88. ncbi Convergent projections of Drosophila olfactory neurons to specific glomeruli in the antennal lobe
    Q Gao
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Neurosci 3:780-5. 2000
    ..This convergence allows for the formation of an odotopic map in the antennal lobe whose organization could provide a basis for olfactory discrimination in Drosophila...
  89. ncbi Knot/Collier and cut control different aspects of dendrite cytoskeleton and synergize to define final arbor shape
    Shiho Jinushi-Nakao
    RIKEN Brain Science Institute, Wako Shi, Saitama 351 0198, Japan
    Neuron 56:963-78. 2007
    ..Hence, this study gives significant general insight into how multiple transcription factors combine to control class-specific dendritic arbor morphology through controlling different aspects of the cytoskeleton...
  90. ncbi Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing
    A Grishok
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 106:23-34. 2001
    ..Our findings suggest that a common processing machinery generates guide RNAs that mediate both RNAi and endogenous gene regulation...
  91. ncbi Microtubule-induced Pins/Galphai cortical polarity in Drosophila neuroblasts
    Sarah E Siegrist
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
    Cell 123:1323-35. 2005
    ..These results identify a role for microtubules in polarizing the neuroblast cortex, a fundamental step for generating cell diversity through asymmetric cell division...
  92. pmc Setting the tempo in development: an investigation of the zebrafish somite clock mechanism
    Francois Giudicelli
    Vertebrate Development Laboratory, Cancer Research UK London Research Institute, London, United Kingdom
    PLoS Biol 5:e150. 2007
    ....
  93. ncbi Loss of LRRK2/PARK8 induces degeneration of dopaminergic neurons in Drosophila
    Sung Bae Lee
    National Creative Research Initiatives Center for Cell Growth Regulation and Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373 1 Kusong Dong, Yusong gu, Taejon, Republic of Korea
    Biochem Biophys Res Commun 358:534-9. 2007
    ..Collectively, our findings unprecedentedly show in vivo that LRRK2 is critical for the integrity of dopaminergic neurons and intact locomotive activity in Drosophila...
  94. ncbi The Drosophila islet gene governs axon pathfinding and neurotransmitter identity
    S Thor
    Molecular Neurobiology Laboratory, The Salk Institute, San Diego, California 92186, USA
    Neuron 18:397-409. 1997
    ..Ectopic expression of islet induces both specific alterations in pathfinding and changes in neurotransmitter identity. These findings indicate that islet coordinately controls two distinct aspects of neuronal identity...
  95. pmc The Drosophila melanogaster Cajal body
    Ji Long Liu
    Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218, USA
    J Cell Biol 172:875-84. 2006
    ..melanogaster. The identification of these nuclear bodies now permits a broad range of questions to be asked about CB structure and function in a genetically tractable organism...
  96. pmc Functional screening identifies miR-315 as a potent activator of Wingless signaling
    Serena J Silver
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:18151-6. 2007
    ..In summary, activity-based screening can selectively identify miRNAs whose deregulation can lead to interpretable phenotypic consequences...
  97. ncbi A distinct small RNA pathway silences selfish genetic elements in the germline
    Vasily V Vagin
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 313:320-4. 2006
    ..Our data suggest that rasiRNAs protect the fly germline through a silencing mechanism distinct from both the miRNA and RNA interference pathways...
  98. ncbi Live imaging of lymphatic development in the zebrafish
    Karina Yaniv
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, 6B 309, 6 Center Drive, Bethesda, Maryland 20892, USA
    Nat Med 12:711-6. 2006
    ..Our results show lymphatic endothelial cells of the thoracic duct arise from primitive veins through a novel and unexpected pathway...
  99. ncbi Stable integration and conditional expression of electroporated transgenes in chicken embryos
    Yuki Sato
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara, 630 0192, Japan
    Dev Biol 305:616-24. 2007
    ..Thus, the techniques proposed in this study provide a novel approach to study the mechanisms of late organogenesis, for which chickens are most suitable model animals...
  100. pmc Post-integration stability of piggyBac in Aedes aegypti
    Nagaraja Sethuraman
    Center for Biosystems Research, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Insect Biochem Mol Biol 37:941-51. 2007
    ..The post-integration mobility properties of piggyBac in Ae. aegypti enhance the utility of this gene vector for certain applications, particularly those where any level of vector remobilization is unacceptable...
  101. pmc DMY gene induces male development in genetically female (XX) medaka fish
    Masaru Matsuda
    Precursory Research for Embryonic Science and Technology PRESTO, Japan Science and Technology Agency, Kawaguchi 332 0012, Japan
    Proc Natl Acad Sci U S A 104:3865-70. 2007
    ..Our data indicate that DMY is an additional sex-determining gene in vertebrates...

Research Grants63

  1. Targeted delivery of butyrate to the colon in mice
    SHARON FLEMING; Fiscal Year: 2006
    ..allowing the influence of butyrate on colonic disease to be studied in vivo and, eventually, with genetically modified animals, facilitating mechanistic studies...
  2. Transgenesis and Xenotransplantation
    Jorge A Piedrahita; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The ability to efficiently modify the swine genome to generate genetically modified animals has the potential to provide a novel source of cells/tissues for clinical applications...
  3. Targeted genome modification of guinea pig and sheep using engineered zinc finger
    Hilda Holcombe; Fiscal Year: 2011
    ..here is to use ZFN technology in the development of reliable and cost-effective methods for producing genetically modified animals that is applicable across species...
  4. Targeted genome modification of guinea pig and sheep using engineered zinc finger
    Hilda Holcombe; Fiscal Year: 2012
    ..here is to use ZFN technology in the development of reliable and cost-effective methods for producing genetically modified animals that is applicable across species...
  5. The Role of AP-1 in the Gonadotrope
    Djurdjica Coss; Fiscal Year: 2013
    ..aim of this proposal, I will determine the function of AP-1 in reproduction in vivo with the use of genetically modified animals. First, I will assess reproductive function and gonadotropin expression in c-Fos deficient animals and ..
  6. Regulation and Function of Tissue Kallikrein
    Julie Chao; Fiscal Year: 2012
    ..mechanisms of tissue kallikrein in cardiac protection after myocardial infarction in normal and genetically modified animals. Our recent studies have shown that tissue kallikrein gene or protein delivery protects against organ ..
  7. Animal Models and Drug Discovery
    Garret A Fitzgerald; Fiscal Year: 2011
    ..broadly applicable new technologies in bioimaging, biosimulation, and bioinformatics;the generation of genetically modified animals and emerging non-rodent models;and the use of embryonic stem cells, patient-specific induced ..
  8. Cytokine regulation of memory CD8 T cell responses
    Kimberly D Klonowski; Fiscal Year: 2013
    ..In Aim 2 we will use both in vitro culture systems and genetically modified animals to determine how populations of TSLP-conditioned DCs affect CD8 T cell priming, memory development, and ..
  9. Striatin, Aldosterone and Hypertension
    Gordon H Williams; Fiscal Year: 2013
    ..two goals will be assessed in Three SPECIFIC AIMS using a three-level translational approach - humans, genetically modified animals, and cells...
  10. ETHANOL SENSITIVITY OF NATIVE AND CLONED NMDA RECEPTORS
    John J Woodward; Fiscal Year: 2013
    ..Experiments in this aim will utilize expression and recording techniques coupled with the use of genetically modified animals. Aim 3 will test the hypothesis that the behavioral responses to alcohol can be modified by expression ..
  11. 2010 Cardiac Regulatory Mechanisms Gordon Research Conference
    Joan Heller Brown; Fiscal Year: 2010
    ..The increased use of genetically modified animals and the development of defined cardiovascular phenotypes has made the cardiovascular system tractable ..
  12. Endogenous Cannabinoids and Brain Function
    Aron H Lichtman; Fiscal Year: 2013
    ..These genetically modified animals, along with the synthetic probes and newly discovered endocannabinoids, will be used to further ..
  13. Rapid Genetic Engineering of Stem Cells
    Nancy L Allbritton; Fiscal Year: 2012
    ..has been assembled to develop a single platform to generate the gene-targeted cells needed to produce genetically modified animals. This platform will integrate state-of-the-art microtechnologies consisting of microfabricated cell ..
  14. Regulation of mucosal memory CD8 T cells by Thymic Stromal Lymphopoietin
    Kimberly D Klonowski; Fiscal Year: 2010
    ..In Aim 2 we will use both in vitro culture systems and genetically modified animals to determine how populations of TSLP-conditioned DCs affect CD8 T cell priming, memory development, and ..
  15. 2012 Cardiac Regulatory Mechanisms Gordon Research Conference and Gordon Research
    Mark E Anderson; Fiscal Year: 2012
    ..The increased use of genetically modified animals and the development of defined cardiovascular phenotypes has made the cardiovascular system tractable ..
  16. Spinal interneurons: 5-HT-NA receptors, actions circuits
    Elzbieta Jankowska; Fiscal Year: 2012
    ..with motor disorders and provide a reference for studies at different developmental stages and in genetically modified animals. PUBLIC HEALTH RELEVANCE: The results of the study should improve our understanding of how cerebellar ..
  17. Neural Mechanisms of Habit Formation and Maintenance
    Henry Yin; Fiscal Year: 2013
    ..with in vivo multi-electrode and ex vivo whole-cell patch-clamp electrophysiological techniques in genetically modified animals. The proposed experiments will also examine mechanisms underlying habit formation at multiple levels of ..
  18. SUR1 (ABCC8) AND ATRIAL KATP CHANNELS
    Colin G Nichols; Fiscal Year: 2013
    ..subunit expression and consequent functional differences within the mouse heart based on analysis of genetically modified animals, and encompass both mechanistic studies of expression control, and studies of the functional ..
  19. RENOVATION OF FACILITIES FOR SWINE BREEDING
    Craig Beattie; Fiscal Year: 2003
    ..In addition, investigators often use genetically modified animals that may be at increased risk of infectious agents, particularly into a breeding population(s)...
  20. UNIV. OF NEVADA RENO ANIMAL FACILITY IMPROVEMENT PROJECT
    WILLIAM MASTERS; Fiscal Year: 2004
    ..These models often involve specialized mutant or genetically modified animals. These valuable animals are often difficult to replace...
  21. Postnatal development of neurohumoral signaling mechanisms in the gut
    Xiaochun Bian; Fiscal Year: 2010
    ..Mice will also be used in some studies to take advantage of genetically modified animals to test our hypothesis. We will use wild type and tryptophan hydroxylase 1 (TpH1) gene knockout mice...
  22. Role of Nuclear Factor TOX in Germinal Center Reactions
    Jonathan Kaye; Fiscal Year: 2009
    ..Using combinations of these genetically modified animals, we propose here to determine whether TOX also plays a role in germinal center reactions...
  23. CONFERENCE ON CHEMOKINES AND CHEMOKINE RECEPTORS
    Gregory LaRosa; Fiscal Year: 2001
    ..Recently, disease model studies using either genetically modified animals, or treatment with chemokine or receptor blocking agents, have provided in vivo proof of the ..
  24. Chemokines in a novel murine model of DTH in the brain
    Richard Ransohoff; Fiscal Year: 2004
    ..Mouse DTH model will enable us to use several murine reagents not available for rats and use genetically modified animals in the future. 2) Analysis of chemokines and chemokine receptor expression in brain DTH model...
  25. A Novel Mini Gamma Camera for in Vivo Molecular Imaging
    Shi Yin; Fiscal Year: 2003
    Development of genetically modified animals, especially mice and rats has opened a new field of testing and understanding for human diseases and also a new related field of molecular imaging...
  26. CELLULAR FUNCTION OF THE JUXTAGLOMERULAR APPARATUS
    LINDA SAMUELSON; Fiscal Year: 1999
    ..kidney at both the single nephron and organ level, in order to lay the foundation for later studies in genetically modified animals. The second aim is to study the effect of disruption in the regulation of angiotensin and of macula ..
  27. High-End Instrumentation Grant Program
    Cheuk Tang; Fiscal Year: 2009
    ..purpose of this instrument is to allow researchers at our institute to study morphology of wild-type or genetically modified animals, study preclinical drug development or other treatment strategies, detect biomarkers of disease and to ..
  28. AGE RELATED DEGENERATION
    Charles Epstein; Fiscal Year: 2002
    ..b>Genetically modified animals have been used for this purpose and have been found to be a particularly powerful method for producing ..
  29. Vevo 770 High Resolution Ultrasound Imaging System
    Marielle Scherrer Crosbie; Fiscal Year: 2007
    ..We will use this machine to better understand the pathways involved in pulmonary and systemic high blood pressure, atherosclerosis, heart failure, as well as to attempt to image stem cells. [unreadable] [unreadable] [unreadable]..
  30. Mechanisms of Redox Mediated Cardioprotection
    Daret St Clair; Fiscal Year: 2006
    ..b>Genetically modified animals will be used to investigate the link between TNF/TAM and mitochondrial antioxidant status in ..
  31. ANIMAL FACILITIES RENOVATION TO ENHANCE RESEARCH
    RICHARD HUNEKE; Fiscal Year: 2003
    ..This has delayed wider incorporation of genetically modified animals into the research. Finally, space for storing racks and cages is urgently needed...
  32. ENDOTHELIN REGULATION OF KIDNEY FUNCTION
    David Pollock; Fiscal Year: 2007
    ..We will use both pharmacological tools and genetically modified animals to elucidate the pathways described in the above scheme...
  33. CD154-CD13 Pathway in Inflammation-driven Angiogenesis
    Linda Shapiro; Fiscal Year: 2004
    ..infarction and ischemia/reperfusion injury will be evaluated in murine models of myocardial disease in genetically modified animals in vivo...
  34. Blocking airway inflammation with B7-DC cross-linking Ab
    Larry Pease; Fiscal Year: 2009
    ..antibodies will be explored by assessing functional changes in DC and T cells isolated from treated and genetically modified animals and by adoptive transfer of manipulated cells into sensitized or naTve hosts...
  35. Development of Functional MRI for Mouse Phenotyping
    Elizabeth Yoder; Fiscal Year: 2005
    ..The endpoint of the project will be the submission of a novel phenotyping protocol to the Mouse Phenome Database. ..
  36. Affymetrix GCS3000 Instrument System
    Nadereh Jafari; Fiscal Year: 2004
    ..in response to specific hormonal and physiological stimuli, and in pathological and disease states, in genetically modified animals, and pharmacogenomics represents an important technological advance...
  37. Targeted gene delivery into whole, adult animals
    PATRICK FOGARTY; Fiscal Year: 2003
    ..application potential for this vector technology includes human gene therapy and rapid production of genetically modified animals (e.g., therapeutic protein production, agriculture improvement, more relevant disease models, etc.)...
  38. A New CT System for Small-Animal Imaging
    MARTIN CLAJUS; Fiscal Year: 2005
    ..high spatial resolution in the mouse; this is important for evaluating phenotype changes that occur in genetically modified animals. Use of micro-CT is also valuable in evaluating tumor kinetics, i.e...
  39. Pathways to Alcohol Tolerance
    Boris Tabakoff; Fiscal Year: 2006
    ..In all, our studies should provide an excellent foundation for understanding the neuroadaptive processes leading to ethanol tolerance in reference to the anxiolytic action of this drug. ..
  40. Ethanol sensitivity--native /recombinant NMDA receptors
    John Woodward; Fiscal Year: 2006
    ..ethanol sensitivity into neurons grown in culture and in vivo; 2) learn methods used to generate genetically modified animals that express mutant NMDA receptor subunits with altered ethanol sensitivity; 3) learn methods to ..
  41. DERIVATION OF RAT EMBRYONIC STEM CELL LINES
    Jeanne Loring; Fiscal Year: 1992
    ..In phase II, rat genes will be targeted through homologous recombination to genetically alter the ES cells, and these cells will be used to generate germ line chimeras. The chimeras will be bred to produce genetically modified animals.
  42. Reproduction of Transgenic Pigs by Nuclear Transplantation
    Randall S Prather; Fiscal Year: 2010
    ..The third aim is to determine the methylation profile and transcriptional profile of two different types of stem cells as compared to fetal-derived fibroblast-like cells. ..
  43. Transgenic chickens for protein production
    Robert Etches; Fiscal Year: 2004
    ..These attributes make chickens an attractive option for the large-scale production of therapeutic proteins such as monoclonal antibodies. ..
  44. IDENTIFICATION OF STEM CELL MARKERS IN MAMMALIAN TESTES
    Ina Dobrinski; Fiscal Year: 2002
    ..abstract_text> ..
  45. Developmental Compartments of the Zebrafish Neurocranium
    Johann Eberhart; Fiscal Year: 2005
    ..Last, a forward genetic screen will be used to discover new mutations that affect the development of neurocranial cartilages. ..
  46. CD40L IN ALLOGRAFT TOLERANCE AND VIRAL IMMUNITY
    Richard Pierson; Fiscal Year: 2001
    ..Finally, this study will investigate the potential of CD40L inhibition to induce prolonged acceptance of a transplanted organ, and may contribute to development of clinically relevant approaches to the induction of tolerance. ..
  47. Multianalyte Assays for Cardiovascular Disease
    Debra Diz; Fiscal Year: 2005
    ..As such the peptide and protein applications developed in this proposal will be ideal for widespread use. ..
  48. Synaptic Alterations in the Cerebral Cortex during Aging
    A Cuello; Fiscal Year: 2005
    ..abstract_text> ..
  49. Excellence in Cardiovascular Sciences Summer Research
    Debra Diz; Fiscal Year: 2007
    ..Moreover, all students regarded the experience as a positive influence on their career and 76% of those who responded said they plan to pursue some aspect of biomedical research as a career. ..
  50. Transgenic Goats Produced by Germ Cell Transplantation
    Ina Dobrinski; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  51. Tolerance to Carbohydrate Antigens in Transplantation
    David Cooper; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  52. Thromboregulation to Prevent Thrombotic Microangiopathy
    David Cooper; Fiscal Year: 2008
    ..It is anticipated that these approaches will abrogate the development of TM and allow long-term survival of pig organs in baboons. ..
  53. Genetic Hierarchies and Cellular Behaviors during Zebrafish Palatogenesis
    Johann Eberhart; Fiscal Year: 2008
    ..Additionally, two genes I propose to analyze, pitx2 and ephrin-B1, are known to be human craniofacial disease genes. Therefore, my analyses of these genes will provide direct insight into the cause of human disease. ..
  54. Control of V(D)J Recombination at the TCR beta Locus
    Jianzhu Chen; Fiscal Year: 2007
    ..The proposed studies may help elucidate the role of enhancers in regulating DSB repair, V(D)J recombination, genome instability, and tumorigenesis. ..
  55. Role of T Cells in Experimental Spondyloarthropathy
    JOEL TAUROG; Fiscal Year: 2002
    ..These experiments will examine directly the effect of Tap2a on the B27 peptide repertoire and on disease manifestations. ..
  56. Molecular Basis of K+ Current During Secretion
    Keith Nehrke; Fiscal Year: 2002
    ..Finally, we will look at compensatory changes that occur in the expression of functionally-related ion transport proteins in the transgenic strain. ..
  57. FINE MAPPING OF GENES REGULATING HEAT LOSS
    Daniel Pomp; Fiscal Year: 2002
    ..These efforts will contribute to the long-term battle against human obesity by facilitating improved methods for diagnosis, treatment and prevention. ..
  58. STEROL METABOLISM & DIETARY CHOLESTEROL IN SLO SYNDROME
    WILLIAM CONNOR; Fiscal Year: 2002
    ..Developmental testing will be performed. ..
  59. SPECIFICATION OF FAST AND SLOW MUSCLES IN ZEBRAFISH
    Shao Jun Du; Fiscal Year: 2003
    ..The long-term goal of this study is to elucidate the molecular mechanisms behind determination of muscle-specific cell fate during embryonic development. ..
  60. Zebrafish Tauopathy Model for Drug Screening and Target Validation
    Amy Rubinstein; Fiscal Year: 2006
    ..We propose to do this by developing a new animal model of neurological disease that can be used to screen a larger number of compounds than is currently possible. [unreadable] [unreadable] [unreadable]..
  61. Adenosine in trauma and sepsis
    Gyorgy Hasko; Fiscal Year: 2007
    ..abstract_text> ..
  62. ASCB Summer Meeting: Nuclear Architecture and Disease
    Katherine Wilson; Fiscal Year: 2005
    ..The meeting will foster intense informal discussion in search of common principals and will stimulate interest and progress in this emerging 'post-genomic' field. ..