genetically modified organisms

Summary

Summary: Organisms whose GENOME has been changed by a GENETIC ENGINEERING technique.

Top Publications

  1. ncbi Public health issues related with the consumption of food obtained from genetically modified organisms
    Andrea Paparini
    University of Rome Foro Italico IUSM, Rome, Italy
    Biotechnol Annu Rev 10:85-122. 2004
  2. ncbi On a salmon (Oncorhynchus [corrected] keta) liver RNase, belonging to RNase T2 family: primary structure and some properties
    Rie Suzuki
    Department of Applied Microbiology, Hoshi University, Tokyo, Japan
    Biosci Biotechnol Biochem 69:343-52. 2005
  3. ncbi Pleurotus sajor-caju HSP100 complements a thermotolerance defect in hsp104 mutant Saccharomyces cerevisiae
    Jin Ohk Lee
    National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441 707, Korea
    J Biosci 31:223-33. 2006
  4. ncbi Genetically modified organisms: do the benefits outweigh the risks?
    Kristina Hug
    K Hug, Department of Medical Ethics, Lund University, BMC C 13, 221 84 Lund, Sweden
    Medicina (Kaunas) 44:87-99. 2008
  5. ncbi Testing for genetically modified organisms (GMOs): Past, present and future perspectives
    Arne Holst-Jensen
    Department of Feed and Food Safety, National Veterinary Institute, Ullevaalsveien 68, P O Box 750 Sentrum, 0106 Oslo, Norway
    Biotechnol Adv 27:1071-82. 2009
  6. ncbi PCR technology for screening and quantification of genetically modified organisms (GMOs)
    Arne Holst-Jensen
    National Veterinary Institute, P O Box 8156 Dep, 0033, Oslo, Norway
    Anal Bioanal Chem 375:985-93. 2003
  7. ncbi Screening genetically modified organisms using multiplex-PCR coupled with oligonucleotide microarray
    Jia Xu
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China
    Biosens Bioelectron 22:71-7. 2006
  8. ncbi Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast
    Meng Sun
    Department of Biotechnology, College of Life Science, Zhejiang University, Hangzhou 310027, PR China
    Biotechnol Lett 25:1087-92. 2003
  9. pmc Refactoring bacteriophage T7
    Leon Y Chan
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Syst Biol 1:2005.0018. 2005
  10. ncbi Suggestions for the assessment of the allergenic potential of genetically modified organisms
    Armin Spök
    Inter University Research Centre for Technology, Work, and Culture, Graz, Austria
    Int Arch Allergy Immunol 137:167-80. 2005

Detail Information

Publications224 found, 100 shown here

  1. ncbi Public health issues related with the consumption of food obtained from genetically modified organisms
    Andrea Paparini
    University of Rome Foro Italico IUSM, Rome, Italy
    Biotechnol Annu Rev 10:85-122. 2004
    b>Genetically Modified Organisms (GMOs) are a fact of modern agriculture and a major field of discussion in biotechnology...
  2. ncbi On a salmon (Oncorhynchus [corrected] keta) liver RNase, belonging to RNase T2 family: primary structure and some properties
    Rie Suzuki
    Department of Applied Microbiology, Hoshi University, Tokyo, Japan
    Biosci Biotechnol Biochem 69:343-52. 2005
    ..Based on the results, the role of H104, which has been proposed to be a phosphate binding site with a substrate, was reconsidered, and we proposed a revised role of this His residue in the hydrolysis mechanism of RNase T2 family enzymes...
  3. ncbi Pleurotus sajor-caju HSP100 complements a thermotolerance defect in hsp104 mutant Saccharomyces cerevisiae
    Jin Ohk Lee
    National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441 707, Korea
    J Biosci 31:223-33. 2006
    ..These results indicate that PsHSP100 protein is functional as an HSP100 in yeast and could play and important role in thermotolerance in P. sajor-caju...
  4. ncbi Genetically modified organisms: do the benefits outweigh the risks?
    Kristina Hug
    K Hug, Department of Medical Ethics, Lund University, BMC C 13, 221 84 Lund, Sweden
    Medicina (Kaunas) 44:87-99. 2008
    The objective of this literature review is to analyze the implications of using genetically modified organisms (GMOs) as well as international and European position regarding such organisms.
  5. ncbi Testing for genetically modified organisms (GMOs): Past, present and future perspectives
    Arne Holst-Jensen
    Department of Feed and Food Safety, National Veterinary Institute, Ullevaalsveien 68, P O Box 750 Sentrum, 0106 Oslo, Norway
    Biotechnol Adv 27:1071-82. 2009
    ..To better understand the suitability and limitations of detection methodologies the evolution of transformation processes for creation of GMOs is briefly reviewed...
  6. ncbi PCR technology for screening and quantification of genetically modified organisms (GMOs)
    Arne Holst-Jensen
    National Veterinary Institute, P O Box 8156 Dep, 0033, Oslo, Norway
    Anal Bioanal Chem 375:985-93. 2003
    ..In addition, we will discuss some of the major challenges related to determination of the limits of detection (LOD) and quantification (LOQ), and to validation of methods...
  7. ncbi Screening genetically modified organisms using multiplex-PCR coupled with oligonucleotide microarray
    Jia Xu
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China
    Biosens Bioelectron 22:71-7. 2006
    ..It can detect more than 95% of currently commercial GMO plants and the limits of detection are 0.5% for soybean and 1% for maize. This method is proved to be a new method for routine analysis of GMOs...
  8. ncbi Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast
    Meng Sun
    Department of Biotechnology, College of Life Science, Zhejiang University, Hangzhou 310027, PR China
    Biotechnol Lett 25:1087-92. 2003
    ..These experimental results support the possibility of using transgenic chloroplasts of green alga as a mucosal vaccine source...
  9. pmc Refactoring bacteriophage T7
    Leon Y Chan
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Syst Biol 1:2005.0018. 2005
    ..The viability of our initial design suggests that the genomes encoding natural biological systems can be systematically redesigned and built anew in service of scientific understanding or human intention...
  10. ncbi Suggestions for the assessment of the allergenic potential of genetically modified organisms
    Armin Spök
    Inter University Research Centre for Technology, Work, and Culture, Graz, Austria
    Int Arch Allergy Immunol 137:167-80. 2005
    ..g., food). Although there is almost no scientific evidence that genetically modified organisms (GMOs) exhibit increased allergenicity compared with the corresponding wild type significant concerns ..
  11. ncbi Detection of nonauthorized genetically modified organisms using differential quantitative polymerase chain reaction: application to 35S in maize
    Katarina Cankar
    Department of Plant Physiology and Biotechnology, National Institute of Biology, Vecna pot 111, 1000 Ljubljana, Slovenia
    Anal Biochem 376:189-99. 2008
    Detection of nonauthorized genetically modified organisms (GMOs) has always presented an analytical challenge because the complete sequence data needed to detect them are generally unavailable although sequence similarity to known GMOs ..
  12. ncbi Capillary electrophoresis with electrochemiluminescent detection for highly sensitive assay of genetically modified organisms
    Longhua Guo
    Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002, China
    Anal Chem 81:9578-84. 2009
    ..Results showed that, under the optimal conditions, the proposed method can accurately identifying RRS. The corresponding limit of detection (LOD) was below 0.01% with 35 PCR cycles...
  13. ncbi Advances in molecular techniques for the detection and quantification of genetically modified organisms
    Dimitrios S Elenis
    Department of Chemistry, University of Athens, Athens, 15771, Greece
    Anal Bioanal Chem 392:347-54. 2008
    Progress in genetic engineering has led to the introduction of genetically modified organisms (GMOs) whose genomes have been altered by the integration of a novel sequence conferring a new trait...
  14. ncbi Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplast
    Scott Franklin
    Department of Cell Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Plant J 30:733-44. 2002
    ..reinhardtii chloroplast gene expression...
  15. pmc Reduced TOR signaling extends chronological life span via increased respiration and upregulation of mitochondrial gene expression
    Nicholas D Bonawitz
    Department of Pathology, Yale University School of Medicine, New Haven, CT 06520, USA
    Cell Metab 5:265-77. 2007
    ....
  16. ncbi A phase I trial with transgenic bacteria expressing interleukin-10 in Crohn's disease
    Henri Braat
    Department of Experimental Internal Medicine, Academic Medical Centre, Amsterdam, The Netherlands
    Clin Gastroenterol Hepatol 4:754-9. 2006
    ..However, treatment of patients with a living, genetically modified bacterium raises questions about the safety for human subjects per se and the biologic containment of the transgene...
  17. ncbi Detection and traceability of genetically modified organisms in the food production chain
    M Miraglia
    Istituto Superiore di Sanita, Section of Cereal Chemistry, Laboratory of Food, Viale Regina Elena 299, I 00161 Rome, Italy
    Food Chem Toxicol 42:1157-80. 2004
    Both labelling and traceability of genetically modified organisms are current issues that are considered in trade and regulation...
  18. pmc Metabolism of zearalenone by genetically modified organisms expressing the detoxification gene from Clonostachys rosea
    Naoko Takahashi-Ando
    Laboratory for Remediation Research, Plant Science Center, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Appl Environ Microbiol 70:3239-45. 2004
    ..We therefore used egfp::zhd101 to visually monitor the lactonohydrolase activity in genetically modified organisms and evaluated the usefulness of zhd101 for in vivo detoxification of ZEN. While recombinant E...
  19. ncbi Uncorking the biomanufacturing bottleneck
    Alan Dove
    Nat Biotechnol 20:777-9. 2002
  20. ncbi Whole-cell living biosensors--are they ready for environmental application?
    Hauke Harms
    Department of Environmental Microbiology, UFZ Centre for Environmental Research Leipzig Halle GmbH, Permoserstr 15, 04318, Leipzig, Germany
    Appl Microbiol Biotechnol 70:273-80. 2006
    ..This includes the identification of some popular misconceptions about the qualities and shortcomings of bioreporters...
  21. ncbi New trends in bioanalytical tools for the detection of genetically modified organisms: an update
    Elisa Michelini
    Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy
    Anal Bioanal Chem 392:355-67. 2008
    Despite the controversies surrounding genetically modified organisms (GMOs), the production of GM crops is increasing, especially in developing countries...
  22. ncbi Quartz crystal microbalance (QCM) affinity biosensor for genetically modified organisms (GMOs) detection
    Ilaria Mannelli
    Dipartimento di Chimica, Universita degli Studi di Firenze, Polo Scientifico Via della Lastruccia 3, Sesto Fiorentino Florence 50019, Italy
    Biosens Bioelectron 18:129-40. 2003
    A DNA piezoelectric sensor has been developed for the detection of genetically modified organisms (GMOs)...
  23. pmc The real and perceived risks of genetically modified organisms
    Helge Torgersen
    Institute of Technology Assessment of the Austrian Academy of Sciences in Vienna, Austria
    EMBO Rep 5:S17-21. 2004
  24. ncbi Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast
    Andrea L Manuell
    The Department of Cell Biology and The Skaggs Institute for Chemical Biology, La Jolla, CA 92037, USA
    Plant Biotechnol J 5:402-12. 2007
    ..As Chlamydomonas reinhardtii is an edible alga, production of therapeutic proteins in this organism offers the potential for oral delivery of gut-active proteins, such as M-SAA...
  25. ncbi Genetically modified organisms for the environment: stories of success and failure and what we have learned from them
    Ildefonso Cases
    National Center for Biotechnology, CSIC, Madrid, Spain
    Int Microbiol 8:213-22. 2005
    ..In this review, several valuable spin-offs of past research into genetically modified organisms with environmental applications are discussed, along with the impact of Systems Biology and Synthetic ..
  26. ncbi Design of a DNA chip for detection of unknown genetically modified organisms (GMOs)
    Håvard Nesvold
    Department of Informatics, University of Oslo, Blindern, Norway
    Bioinformatics 21:1917-26. 2005
    Unknown genetically modified organisms (GMOs) have not undergone a risk evaluation, and hence might pose a danger to health and environment. There are, today, no methods for detecting unknown GMOs...
  27. ncbi Toward safe genetically modified organisms through the chemical diversification of nucleic acids
    Piet Herdewijn
    Laboratory for Medicinal Chemistry, Rega Institute for Medical Research, Minderbroedersstraat 10, B 3000 Leuven
    Chem Biodivers 6:791-808. 2009
    ....
  28. ncbi The promise and perils of synthetic biology
    Jonathan B Tucker
    Center for Nonproliferation Studies CNS, Monterey Institute of International Studies, USA
    New Atlantis 12:25-45. 2006
  29. ncbi Crossover assurance and crossover interference are distinctly regulated by the ZMM proteins during yeast meiosis
    Miki Shinohara
    Institute for Protein Research, Osaka University, Suita 565 0871, Japan
    Nat Genet 40:299-309. 2008
    ..In contrast, crossover assurance requires all ZMM proteins and full-length synaptonemal complexes...
  30. pmc Identification of a novel mycobacterial 3-hydroxyacyl-thioester dehydratase, HtdZ (Rv0130), by functional complementation in yeast
    Aner Gurvitz
    Section of Physiology of Lipid Metabolism, Institute of Physiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    J Bacteriol 190:4088-90. 2008
    ..Mutant cells expressing HtdZ contained dehydratase activity, recovered their respiratory ability, and partially restored de novo lipoic acid synthesis...
  31. ncbi Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs)
    I Taverniers
    Department for Plant Genetics and Breeding, CLO-Gent, Caritasstraat 21, B-9090 Melle, Belgium
    Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 66:469-72. 2001
    For the quantification of genetically modified organisms (GMOs) in foods and feeds, real-time PCR is currently the most widely applied technique...
  32. ncbi Traceability of genetically modified organisms
    Henk J M Aarts
    Expert Rev Mol Diagn 2:69-76. 2002
    EU regulations stipulate the labeling of food products containing genetically modified organisms (GMOs) unless the GMO content is due to adventitious and unintended 'contamination' and not exceeding the 1% level at ingredient basis...
  33. ncbi Recombinant luminescent bacterial sensors for the measurement of bioavailability of cadmium and lead in soils polluted by metal smelters
    Angela Ivask
    National Institute of Chemical Physics and Biophysics, Laboratory of Molecular Genetics, Akadeemia tee 23, 12618 Tallinn, Estonia
    Chemosphere 55:147-56. 2004
    ..e., in the contact assay). The bioavailability of metals in different soils varied (depending probably on soil type) ranging from 0.5% to 56% for cadmium and from 0.2% to 8.6% for lead...
  34. ncbi Recombinant collagen and gelatin for drug delivery
    David Olsen
    FibroGen, Inc, 225 Gateway Boulevard, South San Francisco, CA 94080, USA
    Adv Drug Deliv Rev 55:1547-67. 2003
    ....
  35. ncbi Conceptualizing risk assessment methodology for genetically modified organisms
    Ryan A Hill
    Environ Biosafety Res 4:67-70. 2005
  36. pmc A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast
    U Gueldener
    Heinrich Heine Universitat, Institut fur Mikrobiologie, Universitatsstrasse 1, Geb 26 12 01 64, 40225 Dusseldorf, Germany
    Nucleic Acids Res 30:e23. 2002
    ..The set of disruption cassettes and Cre expression plasmids described here represents a significant further development of the marker rescue system, which is ideally suited to functional analysis of the yeast genome...
  37. pmc Identification of a mannitol transporter, AgMaT1, in celery phloem
    N Noiraud
    , , 40 Avenue du Recteur Pineau, F-86022 Poitiers Cedex, France
    Plant Cell 13:695-705. 2001
    ....
  38. ncbi A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress
    Gisela Mir
    Departament de Biologia, Universitat de Girona, Campus Montilivi, E 17071 Girona, Spain
    J Exp Bot 55:2483-93. 2004
    ..The putative role of QsMT in oxidative stress, both as a free radical scavenger via its sulphydryl groups or as a copper chelator is discussed...
  39. ncbi A cyanobacterial protein with similarity to phytochelatin synthases catalyzes the conversion of glutathione to gamma-glutamylcysteine and lacks phytochelatin synthase activity
    Emiko Harada
    Leibniz Institut für Pflanzenbiochemie, Stress and Developmental Biology, Weinberg 3, 06120 Halle Saale, Germany
    Phytochemistry 65:3179-85. 2004
    ..Taken together, these data provide further evidence for possible additional functions of phytochelatin synthase-related proteins in glutathione metabolism and provide a lead as to the evolutionary history of phytochelatin synthesis...
  40. ncbi Expression of a wheat cytochrome P450 monooxygenase in yeast and its inhibition by glyphosate
    Wen Sheng Xiang
    State Key Laboratory for Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Science, Beijing 100080, China
    Pest Manag Sci 61:402-6. 2005
    ..A spectral dissociation constant, K(s) of 70 micromol litre(-1) was observed and an IC50 of 11 micromol litre(-1) was found for glyphosate inhibition of CYP71C6v1 P450 activity...
  41. ncbi Isolation of trehalose-6-phosphate phosphatase gene from tobacco and its functional analysis in yeast cells
    Yu Jun Wang
    National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing 100101, China
    J Plant Physiol 162:215-23. 2005
    ..When expressing in yeast tps2 mutant, NtTPPL rescued the mutant phenotype under high temperature. This result indicated that NtTPPL functioned as a trehalose-6-phosphate phosphatase in yeast and may play similar roles in plants...
  42. pmc Identification of Thlaspi caerulescens genes that may be involved in heavy metal hyperaccumulation and tolerance. Characterization of a novel heavy metal transporting ATPase
    Ashot Papoyan
    United States Plant, Soil and Nutrition Laboratory, United States Department of Agriculture, Agricultural Research Service, Cornell University, Ithaca, New York 14853, USA
    Plant Physiol 136:3814-23. 2004
    ..The possibilities for enhancing the metal tolerance and phytoremediation potential of higher plants via expression of these metal-binding peptides are also discussed...
  43. ncbi Engineering of a mammalian O-glycosylation pathway in the yeast Saccharomyces cerevisiae: production of O-fucosylated epidermal growth factor domains
    Yuko Chigira
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1, Higashi, Tsukuba, Ibaraki
    Glycobiology 18:303-14. 2008
    ..Such proteins can be used for the analysis of substrate specificity and the production of antibodies that recognize O-glycosylated EGF domains...
  44. ncbi Purification and immunity analysis of recombinant 6His-HPT protein expressed in E. coli
    Li Chen Yang
    Key Laboratory of Trace Element Nutrition of the Ministry of Health, China, Institute of Nutrition and Food Safety, Chinese Center for Diseases Control and Prevention, Beijing 100050, China
    Biomed Environ Sci 16:149-56. 2003
    ..To obtain HPT protein (Hygromycin B Phosphotransferase), a kind of plant selective maker gene product expressed from E. coli and to prepare the polyclonal antibody (pAbs) against it...
  45. ncbi Chemical sensing of DNT by engineered olfactory yeast strain
    Venkat Radhika
    Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 N Broad Street, Philadelphia, Pennsylvania 19140, USA
    Nat Chem Biol 3:325-30. 2007
    ..With this approach, we have identified the novel rat olfactory receptor Olfr226, which is closely related to the mouse olfactory receptors Olfr2 and MOR226-1, as a 2,4-dinitrotoluene-responsive receptor...
  46. ncbi The strawberry gene Cyf1 encodes a phytocystatin with antifungal properties
    Manuel Martinez
    Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biotecnología UPM, ETSI Agronomos, Ciudad Universitaria s n, E 28040 Madrid, Spain
    J Exp Bot 56:1821-9. 2005
    ..7 x 10(-7) M) and cathepsin B (K(i) 3.3 x 10(-6) M), and was a good inhibitor of the in vitro growth of phytopathogenic fungi Botrytis cinerea (EC(50): 1.90 microM) and Fusarium oxysporum (EC(50): 2.28 microM)...
  47. ncbi Molecular cloning, characterization, and downregulation of an acyltransferase that catalyzes the malonylation of flavonoid and naphthol glucosides in tobacco cells
    Goro Taguchi
    Division of Gene Research, Department of Life Sciences, Research Center for Human and Environmental Sciences, Shinshu University, 3 15 1 Tokida, Ueda 386 8567, Japan
    Plant J 42:481-91. 2005
    ..These results show that NtMaT1 is the main catalyst of malonylation on glucosides of xenobiotic flavonoids and naphthols in tobacco plants...
  48. pmc Cloning and characterization of red clover polyphenol oxidase cDNAs and expression of active protein in Escherichia coli and transgenic alfalfa
    Michael L Sullivan
    United States Dairy Forage Research Center, Agricultural Research Service, United States Department of Agriculture, Madison, Wisconsin 53706, USA
    Plant Physiol 136:3234-44. 2004
    ..Transgenic alfalfa expressing red clover PPO should prove an excellent model system to further characterize the red clover PPO enzymes and PPO-mediated inhibition of postharvest proteolysis in forage plants...
  49. ncbi The heterologous expression of polysaccharidase-encoding genes with oenological relevance in Saccharomyces cerevisiae
    P Van Rensburg
    Department of Viticulture and Oenology, Institute for Wine Biotechnology, Stellenbosch University, Matieland, South Africa
    J Appl Microbiol 103:2248-57. 2007
    ....
  50. pmc Interspecies variation reveals a conserved repressor of alpha-specific genes in Saccharomyces yeasts
    Oliver A Zill
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
    Genes Dev 22:1704-16. 2008
    ..cerevisiae. We suggest that a comprehensive understanding of some genetic pathways may be best attained through the expanded phenotypic space provided by study of those pathways in multiple related organisms...
  51. ncbi The biotrophic, non-appressorium-forming grass pathogen Claviceps purpurea needs a Fus3/Pmk1 homologous mitogen-activated protein kinase for colonization of rye ovarian tissue
    G Mey
    Institut fur Botanik, Westfaelische Wilhelms Universitaet, Muenster, Germany
    Mol Plant Microbe Interact 15:303-12. 2002
    ..Although both fungi drastically differ in their pathogenic strategies, this result indicates that the signal pathway involving CPMK1 is highly conserved...
  52. pmc Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1
    Irina Chernyakov
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Genes Dev 22:1369-80. 2008
    ..The RTD pathway is the first to be implicated in the turnover of mature RNA species from the class of stable RNAs. These results and the results of others demonstrate that tRNA, like mRNA, is subject to multiple quality control steps...
  53. pmc Genome engineering in Bacillus anthracis using Cre recombinase
    Andrei P Pomerantsev
    Bacterial Toxins and Therapeutics Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 4349, USA
    Infect Immun 74:682-93. 2006
    ....
  54. ncbi The human minisatellites MS1, MS32, MS205 and CEB1 integrated into the yeast genome exhibit different degrees of mitotic instability but are all stabilised by RAD27
    Shohreh Maleki
    Department of Genetic and Cellular Toxicology, Stockholm University, S 10691 Stockholm, Sweden
    Curr Genet 41:333-41. 2002
    ....
  55. ncbi Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporum
    Marc Bellion
    Nancy University, Research Unit 1136 INRA UHP Tree Microbe Interactions, BP 239, F 54506 Vandoeuvre les Nancy Cedex, France
    New Phytol 174:151-8. 2007
    ..Additionally, we demonstrated the usefulness of mycorrhizal fungi transformation using Agrobacterium technology to approach gene function...
  56. ncbi Genetic and biochemical studies in yeast reveal that the cotton fibre-specific GhCER6 gene functions in fatty acid elongation
    Yong Mei Qin
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China
    J Exp Bot 58:473-81. 2007
    ..The results suggest that GhCER6 encodes a functional 3-ketoacyl-CoA synthase...
  57. ncbi The NAC domain mediates functional specificity of CUP-SHAPED COTYLEDON proteins
    Ken Ichiro Taoka
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630 0101, Japan
    Plant J 40:462-73. 2004
    ..Taken together, these observations suggest that CUC proteins transactivate the target genes involved in SAM formation and organ separation through a specific interaction between the NAC domain and the promoter region of the target genes...
  58. ncbi [Suppression of nonsense and frameshift mutations obtained by different methods for inactivating the translation termination factor eRF3 in yeast Saccharomyces cerevisiae]
    S P Zadorskiĭ
    Department of Genetics and Breeding, St Petersburg State University, St Petersburg, 199034 Russia
    Genetika 39:489-94. 2003
    ....
  59. ncbi One-pot two-step enzymatic coupling of pyrimidine bases to 2-deoxy-D-ribose-5-phosphate. A new strategy in the synthesis of stable isotope labeled deoxynucleosides
    N Ouwerkerk
    Leiden University, Leiden Institute of Chemistry, Gorlaeus Laboratories, P O Box 9502, 2300 RA Leiden, The Netherlands
    J Org Chem 67:1480-9. 2002
    ....
  60. ncbi The effect of polysaccharide-degrading wine yeast transformants on the efficiency of wine processing and wine flavour
    C Louw
    Institute for Wine Biotechnology, Department of Viticulture and Oenology, Stellenbosch University, Matieland Stellenbosch, ZA 7602, South Africa
    J Biotechnol 125:447-61. 2006
    ..The Pinot Noir wines were also sensorial evaluated and the tasting panel preferred the wines fermented with the recombinant strains...
  61. ncbi Asilomar's legacy in Aotearoa New Zealand
    Tee Rogers-Hayden
    Centre for Environmental Risk, Environmental Sciences, University of East Anglis, Norwich NR4 7TJ, UK
    Sci Cult (Lond) 14:393-410. 2005
  62. ncbi Does proline accumulation play an active role in stress-induced growth reduction?
    Albino Maggio
    Center for Plant Environmental Stress Physiology, Purdue University, 1165 Horticulture Bldg, West Lafayette, Indiana 47907 1165, USA
    Plant J 31:699-712. 2002
    ..As in previous studies with transgenic plants that overaccumulate osmolytes, we observed some increase in relative growth of proline-overaccumulating cells in mild hyperosmotic stress...
  63. pmc Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant tryptophan pathway
    Takuya Kanno
    Cell Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University, Matsuyama 790 8577, Japan
    Plant Physiol 138:2260-8. 2005
    ..Thus, our in vitro approach utilizing structural information of bacterial homologs is a potent technique to generate designer enzymes with predefined functions...
  64. ncbi Genetic engineering regulation in Australia: an 'archaeology' of expertise and power
    Richard Hindmarsh
    Australian School of Environmental Sciences, Griffith University, Nathan, Brisbane, 4111, Australia
    Sci Cult (Lond) 14:373-92. 2005
  65. ncbi The science and politics of plant biotechnology--a personal perspective
    Indra K Vasil
    University of Florida, Box 110690, Gainesville, Florida 32611 0690, USA
    Nat Biotechnol 21:849-51. 2003
  66. ncbi Cloning, expression in yeast, and functional characterization of CYP76A4, a novel cytochrome P450 of petunia that catalyzes (omega-1)-hydroxylation of lauric acid
    Katsutomo Tamaki
    Laboratory of Biotechnology, Hyogo Prefectural Research Institute for Agriculture, Kasai shi, Hyogo, Japan
    Biosci Biotechnol Biochem 69:406-9. 2005
    ..The CYP76A4 cDNA was expressed in yeast Saccharomyces cerevisiae AH22. Recombinant yeast microsomes containing the CYP76A4 hemoprotein were found to catalyze (omega-1)-hydroxylation of lauric acid...
  67. ncbi Copper-dependent production of a Pycnoporus coccineus extracellular laccase in Aspergillus oryzae and Saccharomyces cerevisiae
    Hisashi Hoshida
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube, Japan
    Biosci Biotechnol Biochem 69:1090-7. 2005
    ..Our results indicate that a high copper concentration is required for the production of active laccase in heterologous hosts and that the copper is required for a post-transcriptional process...
  68. ncbi Genetically modified organisms in the United States: implementation, concerns, and public perception
    Max P Oeschger
    Department of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    Adv Biochem Eng Biotechnol 107:57-68. 2007
    ..These crops, and the other, newer GM crops, have produced no problems and have pioneered a silent agricultural revolution in the USA...
  69. ncbi No Munich on GM crops
    Pierre Malvoisin
    Nat Biotechnol 22:1501; discussion 1501. 2004
  70. ncbi Europe sees sharp decline in GMO research
    Peter Mitchell
    Nat Biotechnol 21:468-9. 2003
  71. ncbi Concern about Japan's unclear biotech regulations
    Kazuo N Watanabe
    Nature 421:689. 2003
  72. ncbi Biotechnological production and applications of coenzyme Q10
    Jin Ho Choi
    School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul, 151 742, South Korea
    Appl Microbiol Biotechnol 68:9-15. 2005
    ..The rational design of metabolic pathways in combination with engineering optimization of fermentation processes could facilitate the development of viable bioconversion processes...
  73. ncbi Genetic engineering: unnatural selection
    Allison Snow
    Department of Evolution, Ecology and Organismal Biology, Ohio State University, Columbus, Ohio 43210, USA
    Nature 424:619. 2003
  74. ncbi Metabolic engineering of ketocarotenoids biosynthesis in the unicelullar microalga Chlamydomonas reinhardtii
    Rosa León
    Departamento de Química y Ciencia de Materiales, Facultad de Ciencias Experimentales, Avda Fuerzas Armadas s n, Universidad de Huelva, 21007 Huelva, Spain
    J Biotechnol 130:143-52. 2007
    ..Surprisingly this ketocarotenoid was not astaxanthin nor canthaxanthin. The ketocarotenoid was identified on the basis of its mass spectrum as 3,3'-dihydroxy-beta,epsilon-carotene-4-one (4-keto-lutein) or its isomer ketozeaxanthin...
  75. pmc Efficient prenylation by a plant geranylgeranyltransferase-I requires a functional CaaL box motif and a proximal polybasic domain
    D Caldelari
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 3102, USA
    Plant Physiol 126:1416-29. 2001
    ....
  76. ncbi Tyr152 plays a central role in the catalysis of 1-aminocyclopropane-1-carboxylate synthase
    Jian Feng Li
    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, China
    J Exp Bot 56:2203-10. 2005
    ..It is therefore concluded that Tyr152, especially its hydroxyl group, plays an essential role in the catalysis of ACC synthase. Thus, a revised catalytic model is hereby proposed for functional ACC synthase...
  77. ncbi EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesis
    Monica Goicoechea
    Institut Fédératif de Recherches FR40, Unité mixte de Recherches 5546, Université Paul Sabatier Centre National de la Recherche Scientifique, Pole de Biotechnologie Vegetale, 24 Chemin de Borde Rouge, BP 17 Auzeville, 31326 Castanet Tolosan, France
    Plant J 43:553-67. 2005
    ..Together these results suggest a role for EgMYB2 in the co-ordinated control of genes belonging to the monolignol-specific pathway, and therefore in the biosynthesis of lignin and the regulation of secondary cell wall formation...
  78. pmc Circadian regulation of the PhCCD1 carotenoid cleavage dioxygenase controls emission of beta-ionone, a fragrance volatile of petunia flowers
    Andrew J Simkin
    Horticultural Sciences, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611 0690, USA
    Plant Physiol 136:3504-14. 2004
    ..beta-Ionone emission by flowers occurred principally during daylight hours, paralleling PhCCD1 expression in corollas. The results indicate that PhCCD1 activity and beta-ionone emission are likely regulated at the level of transcript...
  79. ncbi A study in scarlet: enzymes of ketocarotenoid biosynthesis in the flowers of Adonis aestivalis
    Francis X Cunningham
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
    Plant J 41:478-92. 2005
    ..It is proposed that a 3,4-desaturation subsequent to 4-hydroxylation of the beta-ring leads to the formation of a 4-keto-beta-ring via an indirect and unexpected route: a keto-enol tautomerization...
  80. ncbi Interaction of Arabidopsis BRASSINOSTEROID-INSENSITIVE 1 receptor kinase with a homolog of mammalian TGF-beta receptor interacting protein
    Hashimul Ehsan
    Department of Horticultural Science, North Carolina State University, Raleigh, NC 27695, USA
    Plant J 43:251-61. 2005
    ..These findings support a role for TRIP-1 in the molecular mechanisms of BR-regulated plant growth and development, possibly as a cytoplasmic substrate of the BRI1 receptor kinase...
  81. ncbi Contribution of conserved Asn residues to the inhibitory activities of Kunitz-type protease inhibitors from plants
    Shiroh Iwanaga
    Laboratory of Biochemistry, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
    Biosci Biotechnol Biochem 69:220-3. 2005
    ..The conserved Asn residues of Kunitz-type inhibitors play an important role in exhibiting effective inhibitory activity by stabilizing the structures of the primary binding loop and protease-inhibitor complex...
  82. ncbi Gene cloning, overproduction, and characterization of thermolabile alkaline phosphatase from a psychrotrophic bacterium
    Yutaka Suzuki
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Japan
    Biosci Biotechnol Biochem 69:364-73. 2005
    ..Comparison of the amino acid sequences of SIB1 and E. coli APases suggests that decreases in electrostatic interactions and number of disulfide bonds are responsible for the cold-adaptation of SIB1 APase...
  83. pmc Diatom plastids possess a phosphoribulokinase with an altered regulation and no oxidative pentose phosphate pathway
    Andreas K Michels
    Institute of Plant Biochemistry, University of Dusseldorf, 40225 Duesseldorf, Germany
    Plant Physiol 137:911-20. 2005
    ..We discuss our results in the context of rearrangements of the subcellular compartmentation of metabolic pathways due to the peculiar evolution of diatoms by secondary endocytobiosis...
  84. ncbi A polymorphic motif in the small subunit of ADP-glucose pyrophosphorylase modulates interactions between the small and large subunits
    Joanna M Cross
    Program in Plant Molecular and Cellular Biology and Horticultural Sciences, University of Florida, PO Box 110690, 2211 Fifield Hall, Gainesville, FL 32611, USA
    Plant J 41:501-11. 2005
    ..Hence, a small polymorphic motif within the small subunit influences both catalytic and allosteric properties by modulating subunit interactions...
  85. ncbi Analysis of parental strain DNA fragments existing in GEMs-Fhhh
    Chun bo Hao
    The National Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science, Nanjing University, Nanjing 210093, China
    J Environ Sci (China) 15:590-4. 2003
    ....
  86. ncbi Development of a real-time PCR method for the differential detection and quantification of four solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum)
    Maher Chaouachi
    Unité Etude du Polymorphisme des Génomes Végétaux EPGV UR1279, Centre National de Génotypage CNG, 2 rue Gaston Crémieux 91057, CP5721, Evry Cedex, France
    J Agric Food Chem 56:1818-28. 2008
    The labeling of products containing genetically modified organisms (GMO) is linked to their quantification since a threshold for the presence of fortuitous GMOs in food has been established...
  87. ncbi Extracellular plant DNA in Geneva groundwater and traditional artesian drinking water fountains
    John Pote
    Environmental Microbial Genomics Group, Laboratoire AMPERE, Ecole Centrale de Lyon, Universite de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully, France
    Chemosphere 75:498-504. 2009
    ..analysis has become central to work on natural gene exchange, forensic analyses, soil bioremediation, genetically modified organisms, exobiology, and palaeontology, fundamental questions about DNA resistance to degradation remain...
  88. ncbi Establishing a risk-assessment process for release of genetically modified wine yeast into the environment
    Heidi Schoeman
    Institute for Wine Biotechnology, Stellenbosch University, Matieland, ZA, South Africa
    Can J Microbiol 55:990-1002. 2009
    The use and release of genetically modified organisms (GMOs) is an issue of intense public concern and, in the case of food and beverages, products containing GMOs or products thereof carry the risk of consumer rejection...
  89. ncbi [Sustainable production of bulk chemicals by application of "white biotechnology"]
    M K Patel
    Department of Science, Technology and Society, Utrecht University, The Netherlands
    Sheng Wu Gong Cheng Xue Bao 24:2022-6. 2008
    ..bacteria and enzymes can be used for White Biotechnology but, according to many experts in the fields, Genetically Modified Organisms (GMO) will be necessary in order to achieve the high yields, concentrations and productivities that ..
  90. ncbi The role of national ethics commissions in Finland
    Ritva Halila
    National Advisory Board on Health Care Ethics, Ministry of Social Affairs and Health, P O Box 33, FIN 00023 Government, Finland
    Bioethics 17:357-68. 2003
    ..works as a national authority and gives binding opinions and recommendations about the use of genetically modified organisms. The Sub-Committee on Medical Research Ethics acts a national research ethics committee and gives ..
  91. pmc Population effects of growth hormone transgenic coho salmon depend on food availability and genotype by environment interactions
    Robert H Devlin
    Fisheries and Oceans Canada, 4160 Marine Drive, West Vancouver, BC, Canada V7V 1N6
    Proc Natl Acad Sci U S A 101:9303-8. 2004
    Environmental risk assessment of genetically modified organisms requires determination of their fitness and invasiveness relative to conspecifics and other ecosystem members...
  92. ncbi [Genetically modified organisms: a new threat to food safety]
    Liliane Spendeler
    Amigos de la Tierra España, Madrid
    Rev Esp Salud Publica 79:271-82. 2005
    This article analyzes all of the food safety-related aspects related to the use of genetically modified organisms into agriculture and food...
  93. ncbi [Genetically modified organisms--problems and legislation]
    J Drobnik
    Cas Lek Cesk 141:107-11. 2002
    b>Genetically modified organisms are defined by law as entities capable of replication and/or transmission of hereditary material that had been altered by the insertion or removal of a DNA fragment...
  94. ncbi The dilemma of dual use biological research: Polish perspective
    Marek Czarkowski
    Department of Internal Medicine and Endocrinology, Medical University of Warsaw, Banacha 1a, 02 097 Warsaw, Poland
    Sci Eng Ethics 16:99-110. 2010
    ..current actions aiming to provide biological safety were based on developing and passing an act about genetically modified organisms (GMO's) and creating a GMO Committee...
  95. ncbi Ethics, evolution and culture
    Alex Mesoudi
    Centre for Applied Ethics, University of British Columbia, 227 6356 Agricultural Rd, Vancouver V6T 1Z2, BC, Canada
    Theory Biosci 127:229-40. 2008
    ..to rapidly changing technological or social environments, such as norms regarding the acceptability of genetically modified organisms. Here we suggest that a science of morality/ethics can benefit from adopting a cultural evolution or ..
  96. ncbi Identification of potentially emerging food safety issues by analysis of reports published by the European Community's Rapid Alert System for Food and Feed (RASFF) during a four-year period
    G A Kleter
    RIKILT Institute of Food Safety, Wageningen University and Research Center, P O Box 230, NL 6700 AE Wageningen, The Netherlands
    Food Chem Toxicol 47:932-50. 2009
    ..Temporary increases were noted in the incidences of the unauthorized dye Para Red, genetically modified organisms, the pesticide isophenfos-methyl, and herring worm, Anisakis simplex...
  97. ncbi Growth of Lactobacillus plantarum in media containing hydrolysates of fish viscera
    S J Horn
    Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, As, Norway
    J Appl Microbiol 99:1082-9. 2005
    ..To compare growth of Lactobacillus plantarum on media containing hydrolysates (peptones) from cod viscera with growth on commercial media...
  98. ncbi A single mutation in P450BM-3 enhances acyl homoserine lactone: acyl homoserine substrate binding selectivity nearly 250-fold
    Puneet K Chowdhary
    Department of Chemistry, University of Texas at Dallas, Richardson, TX 75080, United States
    J Biotechnol 135:374-6. 2008
    ..The enzymatic inactivation of quorum signals has shown promise for use in genetically modified organisms resistant to pathogens...
  99. ncbi Real-time polymerase chain reaction detection of cauliflower mosaic virus to complement the 35S screening assay for genetically modified organisms
    Katarina Cankar
    National Institute of Biology, Department of Plant Physiology and Biotechnology, Vecna pot 111, 1000 Ljubljana, Slovenia
    J AOAC Int 88:814-22. 2005
    Labeling of genetically modified organisms (GMOs) is now in place in many countries, including the European Union, in order to guarantee the consumer's choice between GM and non-GM products...
  100. ncbi Vector-free cloning of a bacterial endo-1,4-beta-glucanase in Lactobacillus plantarum and its effect on the acidifying activity in silage: use of recombinant cellulolytic Lactobacillus plantarum as silage inoculant
    F Rossi
    Dipartimento Scientifico e Tecnologico, Facolta di Scienze MM FF e NN, Universita degli Studi di Verona, Italy
    Antonie Van Leeuwenhoek 80:139-47. 2001
    ..Therefore two different genetically modified organisms (GMOs) per each wild-type (WT), producing 43-59 U/l cellulase in 16 h, were examined...
  101. ncbi Democracy and the governance of uncertainty. The case of agricultural gene technologies
    L Pellizzoni
    Department of Human Sciences, University of Trieste, Piazzale Europa 1, 34127, Trieste, Italy
    J Hazard Mater 86:205-22. 2001
    The use of genetically modified organisms (GMOs) in agriculture and food production is the object of an intense and divisive debate...

Research Grants62

  1. Eradication of a Primary Filariasis Vector Population at an Endemic Field Site
    STEPHEN LEONARD DOBSON; Fiscal Year: 2010
    ..the proposed strategy employs a naturally occurring bacteria infection and does NOT include genetically modified organisms. The preliminary studies section describes how a l/Vo/bac/7/a-infected Ae...
  2. Biological vector control reducing arboviruses, including Dengue and Chikungunya
    JAMES WILLIAM MAINS; Fiscal Year: 2012
    ..albopictus, without involving genetically modified organisms. In brief, the strategy is to release Ae...
  3. NextGen DNA Binding Proteins Based on TAL Domains
    David J Segal; Fiscal Year: 2013
    ..of which have had transformative impact in the areas of gene regulation, genome engineering, genetically modified organisms, and gene therapy...
  4. Reservoirs of Antibiotic Resistance--Bioinformatics
    Michael Feldgarden; Fiscal Year: 2006
    ..prophylaxis and therapy in human disease, animal husbandry and horticulture and in management of genetically modified organisms. The continued growth of the ROAR network, applied to this question, will thus accelerate the pace of ..
  5. Metabolomics and metabolic compartmentation in the brain
    Julian Griffin; Fiscal Year: 2006
    ..To correlate NMR observable metabolite changes with transcriptional changes. Our data will provide proof-of principle for this methodology, and establish a means for exploring other neurolodegenerative disorders. ..
  6. RADIATION INDUCTION OF GENOMIC REARRANGEMENTS IN YEAST
    Michael Fasullo; Fiscal Year: 2004
    ..abstract_text> ..
  7. Activating liver carcinogens in yeast by expressing CYP450 polymorphisms
    Michael Fasullo; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  8. Targeted Transposons for Gene Therapy
    PERRY HACKETT; Fiscal Year: 2007
    ..3: Evaluate the efficiency of a LTT targeting tether to enhance uptake and transposition of a pKLAT2 transposon vector into liver cells in mice. [unreadable] [unreadable] [unreadable]..
  9. Is S-adenosyl Methionine a Nutrition: Genetic Link in AD?
    Thomas Shea; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  10. Newborn screening for PKU and BH4 responsiveness
    Steven Dobrowolski; Fiscal Year: 2008
    ..High resolution melt profiling will play a role to identify BH4 responsive PKU patients. ..
  11. Analysis of Plasmodium development in the mosquito
    Marcelo Jacobs Lorena; Fiscal Year: 2006
    ..Knockout mutations will be produced and changes of gene expression caused by these mutations will be measured by use of the micro-arrays. ..
  12. Species-specific regulated transposition in mosquitoes
    Anthony James; Fiscal Year: 2008
    ..gambiae and Ae. aegypti. ..
  13. IMPDH as a Drug Target in Cryptosporidium
    Boris Striepen; Fiscal Year: 2008
    ..This work will provide urgently needed molecular reagents to understand the biology of nutrient uptake and drug resistance in this unique parasite. [unreadable] [unreadable] [unreadable]..
  14. LIVER REPOPULATION FOR LIVER INJURY AND GENE THERAPY
    Sanjeev Gupta; Fiscal Year: 2007
    ..Our studies will provide valuable preclinical information for eventual applications of cell therapy in people. ..
  15. Genetic Analysis of Plasmodium falciparum Gametocyte Formation
    Marcelo Jacobs Lorena; Fiscal Year: 2007
    ..A better understanding of P. falciparum gametocytogenesis may lead to the development of means to prevent gametocyte formation and thus, block transmission. [unreadable] [unreadable] [unreadable]..
  16. Ninth International Congress on Toxoplasmosis
    Boris Striepen; Fiscal Year: 2007
    ..gondii that is not often seen in fields as high paced and[unreadable] competitive and has facilitated rapid and sustained progress in research on this pathogen[unreadable] and related parasites. ..
  17. Wolbachia as an Agent for Population Replacement in Anopheles Gambiae
    Jason Rasgon; Fiscal Year: 2008
    ..We propose to evaluate transfer Wolbachia infection into Anopheles gambiae and evaluate the symbiont as a candidate transgene drive system for malaria control. [unreadable] [unreadable] [unreadable]..
  18. Sugar signaling networks detected by high content fluxomics
    Wolf B Frommer; Fiscal Year: 2010
    ..The networks and databases developed in this project promise to provide novel screening methods for novel drugs as well as help identifying new drug targets. ..
  19. Evolution of codon usage bias in the genus Drosophila
    Jeffrey Powell; Fiscal Year: 2009
    ..willistoni, one of the species being sequenced. ..
  20. Measuring Entomological Risk for Dengue
    THOMAS WALLACE SCOTT; Fiscal Year: 2010
    ..Our results will be relevant for dengue prevention programs based on vector control and/or vaccine strategies and will have immediate, practical applications for dengue surveillance and control worldwide. ..
  21. MOLECULAR ANALYSIS OF THE MOSQUITO PERITROPHIC MATRIX
    Marcelo Jacobs Lorena; Fiscal Year: 2004
    ..gambiae is likely to become available in the near future. The characterization of PM protein genes and an understanding of their function may lead to novel strategies for malaria control. ..
  22. H. influenzae Genes Associated with Acute Otitis Media
    Janet R Gilsdorf; Fiscal Year: 2010
    ....
  23. Regulation of APP Degradation and ABeta Secretion
    David Schubert; Fiscal Year: 2008
    ..In addition, understanding how cells regulate the degradation of proteins is critical to the study of a wide range of diseases, particularly those in the nervous system that are characterized by the intracellular protein accumulation. ..
  24. LIVER REPOPULATION FOR LIVER INJURY AND GENE THERAPY
    Sanjeev Gupta; Fiscal Year: 2008
    ..Completion of our studies will generate new knowledge in liver repopulation and cell and gene therapy mechanisms. This will lead to more effective liver-directed cell and gene therapy in people. ..
  25. Controlled Diversity in Bacterial Stress Response
    Adam Arkin; Fiscal Year: 2009
    ..abstract_text> ..
  26. Genetic Studies of African Anopheles Vectors of Malaria
    Jeffrey Powell; Fiscal Year: 2009
    ..Replacing natural vector populations with such strains require that the strain have high fitness. ..
  27. Intermediate Filaments 2008 Gordon Research Conference
    Thomas Shea; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  28. Delivery of Sleeping Beauty Transposons to Dog Liver for Gene Therapy
    PERRY HACKETT; Fiscal Year: 2008
    ..The goal of this project is to develop a transposon delivery system that will allow hydrodynamic delivery of SB transposons in dogs as a model large animal for humans. [unreadable] [unreadable] [unreadable]..
  29. MOLECULAR AND FUNCTIONAL STUDIES OF H INFLUENZAE PILI
    Janet Gilsdorf; Fiscal Year: 2001
    ..The receptor binding domain antibodies will be tested for their ability to interfere with the adherence of piliated Hi to human erythrocytes and respiratory cells. ..
  30. Sterol Biosynthesis in Trypanosomatid Parasites
    Frederick Buckner; Fiscal Year: 2004
    ..The drugs discovered in this research program will hopefully provide better future treatment for patients with these devastating diseases. ..
  31. Nanospheres as Vehicles for Treatment of Neuroblastoma
    Thomas Shea; Fiscal Year: 2004
    ..Resulting data will indicate whether or not nanospheres represent useful delivery agents for cancer therapy. ..
  32. Protein targeting and secretion in Toxoplasma
    Boris Striepen; Fiscal Year: 2004
    ..Mutant analysis will also provide critical information towards the function of the individual secretory organelles involved. ..
  33. The two-edged sword: vaccines as weapons
    Janet Gilsdorf; Fiscal Year: 2003
    ....
  34. MULTIPLE BLOOD FEEDING BY MOSQUITOES
    Thomas Scott; Fiscal Year: 2003
    ..Insecticide treatments at the end of each experiment will kill any surviving released mosquitoes before they could become infective and transmit virus. ..
  35. ADAPTING NAGCAT FOR ETHNIC COMMUNITIES : A RESEARCH MODE
    JOHN SHUTSKE; Fiscal Year: 2002
    ....
  36. ENTOMOLOGICAL ASSUMPTIONS OF DENGUE CONTROL
    Thomas Scott; Fiscal Year: 2002
    ....
  37. RECOMBINANT ANTIBODIES BLOCKING SPOROZOITE TRANSMISSION
    Anthony James; Fiscal Year: 2002
    ..The idea is that results from these research projects will be relatively easily transferred to the P. falciparum sporozoites and their vectors, anopholine mosquitoes. ..
  38. PHOSPHATE CONTROL OF THE ESCHERICHIA COLI PHO REGULON
    Barry Wanner; Fiscal Year: 2001
    ..Common to the three aims is the idea that PhoB, PhoR and PhoU interact. ..
  39. Structure Function in Low Resistance Junctions
    Camillo Peracchia; Fiscal Year: 2005
    ..The relevance of a potential CaM binding site identified in connexins (similar to that of CaM kinase II) will be tested with deletion and mutation of crucial Cx32 residues. ..
  40. INTERFERON-B AND COPOLYMER-I IN MULTIPLE SCLEROSIS.
    Suhayl Dhib Jalbut; Fiscal Year: 2003
    ..The proposed research could help identify components of the immunologic network in MS that are associated with response to treatment, and therefore help in the design of more effective future therapies. ..
  41. Comparative Modeling of Neurodegenerative Diseases
    CHRISTOPHER LINK; Fiscal Year: 2006
    ..These studies will directly test whether there is a common underlying toxic mechanism for these neurodegenerative diseases. [unreadable] [unreadable]..
  42. Genomic-driven Approaches in E.coli Physiology
    Barry Wanner; Fiscal Year: 2005
    ....
  43. EXPRESSION OF EXOGENOUS GENES IN VECTOR MOSQUITOES
    Anthony James; Fiscal Year: 2006
    ..stephensi and An. gambiae expressing reporter genes under the control of promoters derived from genes expressed specifically in the adult female fat body cells. ..
  44. H. influenzae genes associated with COPD
    Janet Gilsdorf; Fiscal Year: 2009
    ..These results will lead to a better understanding of the H. influenzae virulence pathways associated with COPD and lead to novel new strategies for prevention and management of COPD. ..
  45. Sensing Biowarfare Agents by Surface Enhanced Raman
    Donald Moir; Fiscal Year: 2005
    ....
  46. GENERATION OF A DELETION PANEL FOR THE ZEBRAFISH GENOME
    Marnie Halpern; Fiscal Year: 2001
    ..As in non-vertebrate genetic systems, a zebrafish deletion panel is an essential resource, providing the necessary bridge between DNA sequence and gene function. ..
  47. A Novel Family of Neuroprotective Compounds for Stroke
    DAVID R SCHUBERT; Fiscal Year: 2010
    ..abstract_text> ..
  48. Type III Secretion Inhibitors for Anti-Infective Therapy
    Donald Moir; Fiscal Year: 2007
    ..aeruginosa TTSS; (3) Screen a diverse compound library to identify and validate TTSS inhibitors; and (4) Prioritize validated screening hits for in vitro potency, mechanism, spectrum, and selectivity. [unreadable] [unreadable]..
  49. GENETIC AND CHEMICAL APPROACHES TO NOVEL LIPID II BINDING PEPTIDE ANTIBIOTICS
    Xihou Yin; Fiscal Year: 2010
    ..Understanding the molecular basis of enduracidin resistance in the producing organism will help predict how new antibiotics may be affected by existing resistance mechanisms and provide insight into ways that resistance may emerge. ..
  50. Redox Sensitive Proteins in Aging and Neurodegeneration
    David Schubert; Fiscal Year: 2006
    ..These proteins, in turn, will be used as targets for the identification of potentially therapeutic antioxidants that could be useful in preventing age-and disease-associated damage to the CNS. ..
  51. Race and Public Communication about Human Variation
    Celeste Condit; Fiscal Year: 2003
    ..The project will focus on the concerns of African Americans about discrimination and genetics and on attitudes about African Americans and genetics held by European Americans and the general population. ..
  52. DNA helicase and primase inhibitors for biodefense
    Donald Moir; Fiscal Year: 2006
    ..Finally, compounds will be tested for lack of toxicity to mammalian cells in culture, and lack of activity against eukaryotic polymerase alpha and helicase, resulting in a collection of validated hits. ..
  53. Lay and Expert Models of Gene-Environment Interaction
    Celeste Condit; Fiscal Year: 2008
    ..This will help recipients to adopt behaviors that will better stave off the risks identified by the tests. [unreadable] [unreadable] [unreadable]..
  54. BIOCOMPLEXITY:ANALYSIS, DESIGN, EVOLUTION-COMPLEX GENES
    Adam Arkin; Fiscal Year: 2004
    ..abstract_text> ..
  55. Genotype Analysis for Diagnosis of Urea Cycle Disorders
    Steven Dobrowolski; Fiscal Year: 2007
    ..The proposed assay panels provide a means to readily assess gene that are well established to contribute to hyperammonemia. [unreadable] [unreadable] [unreadable]..
  56. Elucidating the Function of Novel Epigenetic Element
    HEATHER TRUE KROB; Fiscal Year: 2005
    ....
  57. Discovery of B. pseudomallei Therapeutics for Biodefense
    Donald Moir; Fiscal Year: 2008
    ..pseudomallei targets. In Phase III, a potent, safe, orally active B. pseudomallei inhibitor will be advanced into IND enabling toxicology and safety pharmacology studies and file an IND. [unreadable] [unreadable] [unreadable]..
  58. FilmArray-A Closed System for Multi-Pathogen Screening
    Steven Dobrowolski; Fiscal Year: 2005
    ..Pouches are designed to assay for candidate infectious agents consistent with clinical presentation. ..
  59. Development and Testing of a Novel Neuroprotective Drug
    David Schubert; Fiscal Year: 2006
    ..The results from these studies will determine the therapeutic potential of this novel compound. [unreadable] [unreadable]..
  60. Analysis of ATP7B in Screening for Wilson Disease
    Steven Dobrowolski; Fiscal Year: 2004
    ..Prospective screening for WD improves efficacy of treatment and quality of life for affected patients. Genotyping is the best option to confirm results based upon ceruloplasmin analysis in asymptomatic patients. ..
  61. PHYSIOLOGY OF NERVE, GLIAL AND MUSCLE CELL LINES
    David Schubert; Fiscal Year: 2005
    ..This work is directly relevant to CNS trauma and stroke as well as to all chronic degenerative diseases where oxidative stress is a pathological hallmark. ..