Genomes and Genes
genetically modified organisms
Summary: Organisms whose GENOME has been changed by a GENETIC ENGINEERING technique.
Publications224 found, 100 shown here
- Public health issues related with the consumption of food obtained from genetically modified organismsAndrea Paparini
University of Rome Foro Italico IUSM, Rome, Italy
Biotechnol Annu Rev 10:85-122. 2004b>Genetically Modified Organisms (GMOs) are a fact of modern agriculture and a major field of discussion in biotechnology...
- On a salmon (Oncorhynchus [corrected] keta) liver RNase, belonging to RNase T2 family: primary structure and some propertiesRie Suzuki
Department of Applied Microbiology, Hoshi University, Tokyo, Japan
Biosci Biotechnol Biochem 69:343-52. 2005..Based on the results, the role of H104, which has been proposed to be a phosphate binding site with a substrate, was reconsidered, and we proposed a revised role of this His residue in the hydrolysis mechanism of RNase T2 family enzymes...
- Pleurotus sajor-caju HSP100 complements a thermotolerance defect in hsp104 mutant Saccharomyces cerevisiaeJin Ohk Lee
National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441 707, Korea
J Biosci 31:223-33. 2006..These results indicate that PsHSP100 protein is functional as an HSP100 in yeast and could play and important role in thermotolerance in P. sajor-caju...
- Genetically modified organisms: do the benefits outweigh the risks?Kristina Hug
K Hug, Department of Medical Ethics, Lund University, BMC C 13, 221 84 Lund, Sweden
Medicina (Kaunas) 44:87-99. 2008The objective of this literature review is to analyze the implications of using genetically modified organisms (GMOs) as well as international and European position regarding such organisms.
- Testing for genetically modified organisms (GMOs): Past, present and future perspectivesArne Holst-Jensen
Department of Feed and Food Safety, National Veterinary Institute, Ullevaalsveien 68, P O Box 750 Sentrum, 0106 Oslo, Norway
Biotechnol Adv 27:1071-82. 2009..To better understand the suitability and limitations of detection methodologies the evolution of transformation processes for creation of GMOs is briefly reviewed...
- PCR technology for screening and quantification of genetically modified organisms (GMOs)Arne Holst-Jensen
National Veterinary Institute, P O Box 8156 Dep, 0033, Oslo, Norway
Anal Bioanal Chem 375:985-93. 2003..In addition, we will discuss some of the major challenges related to determination of the limits of detection (LOD) and quantification (LOQ), and to validation of methods...
- Screening genetically modified organisms using multiplex-PCR coupled with oligonucleotide microarrayJia Xu
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China
Biosens Bioelectron 22:71-7. 2006..It can detect more than 95% of currently commercial GMO plants and the limits of detection are 0.5% for soybean and 1% for maize. This method is proved to be a new method for routine analysis of GMOs...
- Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplastMeng Sun
Department of Biotechnology, College of Life Science, Zhejiang University, Hangzhou 310027, PR China
Biotechnol Lett 25:1087-92. 2003..These experimental results support the possibility of using transgenic chloroplasts of green alga as a mucosal vaccine source...
- Refactoring bacteriophage T7Leon Y Chan
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Mol Syst Biol 1:2005.0018. 2005..The viability of our initial design suggests that the genomes encoding natural biological systems can be systematically redesigned and built anew in service of scientific understanding or human intention...
- Suggestions for the assessment of the allergenic potential of genetically modified organismsArmin Spök
Inter University Research Centre for Technology, Work, and Culture, Graz, Austria
Int Arch Allergy Immunol 137:167-80. 2005..g., food). Although there is almost no scientific evidence that genetically modified organisms (GMOs) exhibit increased allergenicity compared with the corresponding wild type significant concerns ..
- Detection of nonauthorized genetically modified organisms using differential quantitative polymerase chain reaction: application to 35S in maizeKatarina Cankar
Department of Plant Physiology and Biotechnology, National Institute of Biology, Vecna pot 111, 1000 Ljubljana, Slovenia
Anal Biochem 376:189-99. 2008Detection of nonauthorized genetically modified organisms (GMOs) has always presented an analytical challenge because the complete sequence data needed to detect them are generally unavailable although sequence similarity to known GMOs ..
- Capillary electrophoresis with electrochemiluminescent detection for highly sensitive assay of genetically modified organismsLonghua Guo
Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002, China
Anal Chem 81:9578-84. 2009..Results showed that, under the optimal conditions, the proposed method can accurately identifying RRS. The corresponding limit of detection (LOD) was below 0.01% with 35 PCR cycles...
- Advances in molecular techniques for the detection and quantification of genetically modified organismsDimitrios S Elenis
Department of Chemistry, University of Athens, Athens, 15771, Greece
Anal Bioanal Chem 392:347-54. 2008Progress in genetic engineering has led to the introduction of genetically modified organisms (GMOs) whose genomes have been altered by the integration of a novel sequence conferring a new trait...
- Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplastScott Franklin
Department of Cell Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
Plant J 30:733-44. 2002..reinhardtii chloroplast gene expression...
- Reduced TOR signaling extends chronological life span via increased respiration and upregulation of mitochondrial gene expressionNicholas D Bonawitz
Department of Pathology, Yale University School of Medicine, New Haven, CT 06520, USA
Cell Metab 5:265-77. 2007....
- A phase I trial with transgenic bacteria expressing interleukin-10 in Crohn's diseaseHenri Braat
Department of Experimental Internal Medicine, Academic Medical Centre, Amsterdam, The Netherlands
Clin Gastroenterol Hepatol 4:754-9. 2006..However, treatment of patients with a living, genetically modified bacterium raises questions about the safety for human subjects per se and the biologic containment of the transgene...
- Detection and traceability of genetically modified organisms in the food production chainM Miraglia
Istituto Superiore di Sanita, Section of Cereal Chemistry, Laboratory of Food, Viale Regina Elena 299, I 00161 Rome, Italy
Food Chem Toxicol 42:1157-80. 2004Both labelling and traceability of genetically modified organisms are current issues that are considered in trade and regulation...
- Metabolism of zearalenone by genetically modified organisms expressing the detoxification gene from Clonostachys roseaNaoko Takahashi-Ando
Laboratory for Remediation Research, Plant Science Center, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
Appl Environ Microbiol 70:3239-45. 2004..We therefore used egfp::zhd101 to visually monitor the lactonohydrolase activity in genetically modified organisms and evaluated the usefulness of zhd101 for in vivo detoxification of ZEN. While recombinant E...
- Uncorking the biomanufacturing bottleneckAlan Dove
Nat Biotechnol 20:777-9. 2002
- Whole-cell living biosensors--are they ready for environmental application?Hauke Harms
Department of Environmental Microbiology, UFZ Centre for Environmental Research Leipzig Halle GmbH, Permoserstr 15, 04318, Leipzig, Germany
Appl Microbiol Biotechnol 70:273-80. 2006..This includes the identification of some popular misconceptions about the qualities and shortcomings of bioreporters...
- New trends in bioanalytical tools for the detection of genetically modified organisms: an updateElisa Michelini
Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy
Anal Bioanal Chem 392:355-67. 2008Despite the controversies surrounding genetically modified organisms (GMOs), the production of GM crops is increasing, especially in developing countries...
- Quartz crystal microbalance (QCM) affinity biosensor for genetically modified organisms (GMOs) detectionIlaria Mannelli
Dipartimento di Chimica, Universita degli Studi di Firenze, Polo Scientifico Via della Lastruccia 3, Sesto Fiorentino Florence 50019, Italy
Biosens Bioelectron 18:129-40. 2003A DNA piezoelectric sensor has been developed for the detection of genetically modified organisms (GMOs)...
- The real and perceived risks of genetically modified organismsHelge Torgersen
Institute of Technology Assessment of the Austrian Academy of Sciences in Vienna, Austria
EMBO Rep 5:S17-21. 2004
- Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplastAndrea L Manuell
The Department of Cell Biology and The Skaggs Institute for Chemical Biology, La Jolla, CA 92037, USA
Plant Biotechnol J 5:402-12. 2007..As Chlamydomonas reinhardtii is an edible alga, production of therapeutic proteins in this organism offers the potential for oral delivery of gut-active proteins, such as M-SAA...
- Genetically modified organisms for the environment: stories of success and failure and what we have learned from themIldefonso Cases
National Center for Biotechnology, CSIC, Madrid, Spain
Int Microbiol 8:213-22. 2005..In this review, several valuable spin-offs of past research into genetically modified organisms with environmental applications are discussed, along with the impact of Systems Biology and Synthetic ..
- Design of a DNA chip for detection of unknown genetically modified organisms (GMOs)Håvard Nesvold
Department of Informatics, University of Oslo, Blindern, Norway
Bioinformatics 21:1917-26. 2005Unknown genetically modified organisms (GMOs) have not undergone a risk evaluation, and hence might pose a danger to health and environment. There are, today, no methods for detecting unknown GMOs...
- Toward safe genetically modified organisms through the chemical diversification of nucleic acidsPiet Herdewijn
Laboratory for Medicinal Chemistry, Rega Institute for Medical Research, Minderbroedersstraat 10, B 3000 Leuven
Chem Biodivers 6:791-808. 2009....
- The promise and perils of synthetic biologyJonathan B Tucker
Center for Nonproliferation Studies CNS, Monterey Institute of International Studies, USA
New Atlantis 12:25-45. 2006
- Crossover assurance and crossover interference are distinctly regulated by the ZMM proteins during yeast meiosisMiki Shinohara
Institute for Protein Research, Osaka University, Suita 565 0871, Japan
Nat Genet 40:299-309. 2008..In contrast, crossover assurance requires all ZMM proteins and full-length synaptonemal complexes...
- Identification of a novel mycobacterial 3-hydroxyacyl-thioester dehydratase, HtdZ (Rv0130), by functional complementation in yeastAner Gurvitz
Section of Physiology of Lipid Metabolism, Institute of Physiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
J Bacteriol 190:4088-90. 2008..Mutant cells expressing HtdZ contained dehydratase activity, recovered their respiratory ability, and partially restored de novo lipoic acid synthesis...
- Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs)I Taverniers
Department for Plant Genetics and Breeding, CLO-Gent, Caritasstraat 21, B-9090 Melle, Belgium
Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 66:469-72. 2001For the quantification of genetically modified organisms (GMOs) in foods and feeds, real-time PCR is currently the most widely applied technique...
- Traceability of genetically modified organismsHenk J M Aarts
Expert Rev Mol Diagn 2:69-76. 2002EU regulations stipulate the labeling of food products containing genetically modified organisms (GMOs) unless the GMO content is due to adventitious and unintended 'contamination' and not exceeding the 1% level at ingredient basis...
- Recombinant luminescent bacterial sensors for the measurement of bioavailability of cadmium and lead in soils polluted by metal smeltersAngela Ivask
National Institute of Chemical Physics and Biophysics, Laboratory of Molecular Genetics, Akadeemia tee 23, 12618 Tallinn, Estonia
Chemosphere 55:147-56. 2004..e., in the contact assay). The bioavailability of metals in different soils varied (depending probably on soil type) ranging from 0.5% to 56% for cadmium and from 0.2% to 8.6% for lead...
- Recombinant collagen and gelatin for drug deliveryDavid Olsen
FibroGen, Inc, 225 Gateway Boulevard, South San Francisco, CA 94080, USA
Adv Drug Deliv Rev 55:1547-67. 2003....
- Conceptualizing risk assessment methodology for genetically modified organismsRyan A Hill
Environ Biosafety Res 4:67-70. 2005
- A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeastU Gueldener
Heinrich Heine Universitat, Institut fur Mikrobiologie, Universitatsstrasse 1, Geb 26 12 01 64, 40225 Dusseldorf, Germany
Nucleic Acids Res 30:e23. 2002..The set of disruption cassettes and Cre expression plasmids described here represents a significant further development of the marker rescue system, which is ideally suited to functional analysis of the yeast genome...
- Identification of a mannitol transporter, AgMaT1, in celery phloemN Noiraud
, , 40 Avenue du Recteur Pineau, F-86022 Poitiers Cedex, France
Plant Cell 13:695-705. 2001....
- A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stressGisela Mir
Departament de Biologia, Universitat de Girona, Campus Montilivi, E 17071 Girona, Spain
J Exp Bot 55:2483-93. 2004..The putative role of QsMT in oxidative stress, both as a free radical scavenger via its sulphydryl groups or as a copper chelator is discussed...
- A cyanobacterial protein with similarity to phytochelatin synthases catalyzes the conversion of glutathione to gamma-glutamylcysteine and lacks phytochelatin synthase activityEmiko Harada
Leibniz Institut für Pflanzenbiochemie, Stress and Developmental Biology, Weinberg 3, 06120 Halle Saale, Germany
Phytochemistry 65:3179-85. 2004..Taken together, these data provide further evidence for possible additional functions of phytochelatin synthase-related proteins in glutathione metabolism and provide a lead as to the evolutionary history of phytochelatin synthesis...
- Expression of a wheat cytochrome P450 monooxygenase in yeast and its inhibition by glyphosateWen Sheng Xiang
State Key Laboratory for Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Science, Beijing 100080, China
Pest Manag Sci 61:402-6. 2005..A spectral dissociation constant, K(s) of 70 micromol litre(-1) was observed and an IC50 of 11 micromol litre(-1) was found for glyphosate inhibition of CYP71C6v1 P450 activity...
- Isolation of trehalose-6-phosphate phosphatase gene from tobacco and its functional analysis in yeast cellsYu Jun Wang
National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing 100101, China
J Plant Physiol 162:215-23. 2005..When expressing in yeast tps2 mutant, NtTPPL rescued the mutant phenotype under high temperature. This result indicated that NtTPPL functioned as a trehalose-6-phosphate phosphatase in yeast and may play similar roles in plants...
- Identification of Thlaspi caerulescens genes that may be involved in heavy metal hyperaccumulation and tolerance. Characterization of a novel heavy metal transporting ATPaseAshot Papoyan
United States Plant, Soil and Nutrition Laboratory, United States Department of Agriculture, Agricultural Research Service, Cornell University, Ithaca, New York 14853, USA
Plant Physiol 136:3814-23. 2004..The possibilities for enhancing the metal tolerance and phytoremediation potential of higher plants via expression of these metal-binding peptides are also discussed...
- Engineering of a mammalian O-glycosylation pathway in the yeast Saccharomyces cerevisiae: production of O-fucosylated epidermal growth factor domainsYuko Chigira
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1, Higashi, Tsukuba, Ibaraki
Glycobiology 18:303-14. 2008..Such proteins can be used for the analysis of substrate specificity and the production of antibodies that recognize O-glycosylated EGF domains...
- Purification and immunity analysis of recombinant 6His-HPT protein expressed in E. coliLi Chen Yang
Key Laboratory of Trace Element Nutrition of the Ministry of Health, China, Institute of Nutrition and Food Safety, Chinese Center for Diseases Control and Prevention, Beijing 100050, China
Biomed Environ Sci 16:149-56. 2003..To obtain HPT protein (Hygromycin B Phosphotransferase), a kind of plant selective maker gene product expressed from E. coli and to prepare the polyclonal antibody (pAbs) against it...
- Chemical sensing of DNT by engineered olfactory yeast strainVenkat Radhika
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 N Broad Street, Philadelphia, Pennsylvania 19140, USA
Nat Chem Biol 3:325-30. 2007..With this approach, we have identified the novel rat olfactory receptor Olfr226, which is closely related to the mouse olfactory receptors Olfr2 and MOR226-1, as a 2,4-dinitrotoluene-responsive receptor...
- The strawberry gene Cyf1 encodes a phytocystatin with antifungal propertiesManuel Martinez
Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biotecnología UPM, ETSI Agronomos, Ciudad Universitaria s n, E 28040 Madrid, Spain
J Exp Bot 56:1821-9. 2005..7 x 10(-7) M) and cathepsin B (K(i) 3.3 x 10(-6) M), and was a good inhibitor of the in vitro growth of phytopathogenic fungi Botrytis cinerea (EC(50): 1.90 microM) and Fusarium oxysporum (EC(50): 2.28 microM)...
- Molecular cloning, characterization, and downregulation of an acyltransferase that catalyzes the malonylation of flavonoid and naphthol glucosides in tobacco cellsGoro Taguchi
Division of Gene Research, Department of Life Sciences, Research Center for Human and Environmental Sciences, Shinshu University, 3 15 1 Tokida, Ueda 386 8567, Japan
Plant J 42:481-91. 2005..These results show that NtMaT1 is the main catalyst of malonylation on glucosides of xenobiotic flavonoids and naphthols in tobacco plants...
- Cloning and characterization of red clover polyphenol oxidase cDNAs and expression of active protein in Escherichia coli and transgenic alfalfaMichael L Sullivan
United States Dairy Forage Research Center, Agricultural Research Service, United States Department of Agriculture, Madison, Wisconsin 53706, USA
Plant Physiol 136:3234-44. 2004..Transgenic alfalfa expressing red clover PPO should prove an excellent model system to further characterize the red clover PPO enzymes and PPO-mediated inhibition of postharvest proteolysis in forage plants...
- The heterologous expression of polysaccharidase-encoding genes with oenological relevance in Saccharomyces cerevisiaeP Van Rensburg
Department of Viticulture and Oenology, Institute for Wine Biotechnology, Stellenbosch University, Matieland, South Africa
J Appl Microbiol 103:2248-57. 2007....
- Interspecies variation reveals a conserved repressor of alpha-specific genes in Saccharomyces yeastsOliver A Zill
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
Genes Dev 22:1704-16. 2008..cerevisiae. We suggest that a comprehensive understanding of some genetic pathways may be best attained through the expanded phenotypic space provided by study of those pathways in multiple related organisms...
- The biotrophic, non-appressorium-forming grass pathogen Claviceps purpurea needs a Fus3/Pmk1 homologous mitogen-activated protein kinase for colonization of rye ovarian tissueG Mey
Institut fur Botanik, Westfaelische Wilhelms Universitaet, Muenster, Germany
Mol Plant Microbe Interact 15:303-12. 2002..Although both fungi drastically differ in their pathogenic strategies, this result indicates that the signal pathway involving CPMK1 is highly conserved...
- Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1Irina Chernyakov
Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
Genes Dev 22:1369-80. 2008..The RTD pathway is the first to be implicated in the turnover of mature RNA species from the class of stable RNAs. These results and the results of others demonstrate that tRNA, like mRNA, is subject to multiple quality control steps...
- Genome engineering in Bacillus anthracis using Cre recombinaseAndrei P Pomerantsev
Bacterial Toxins and Therapeutics Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 4349, USA
Infect Immun 74:682-93. 2006....
- The human minisatellites MS1, MS32, MS205 and CEB1 integrated into the yeast genome exhibit different degrees of mitotic instability but are all stabilised by RAD27Shohreh Maleki
Department of Genetic and Cellular Toxicology, Stockholm University, S 10691 Stockholm, Sweden
Curr Genet 41:333-41. 2002....
- Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporumMarc Bellion
Nancy University, Research Unit 1136 INRA UHP Tree Microbe Interactions, BP 239, F 54506 Vandoeuvre les Nancy Cedex, France
New Phytol 174:151-8. 2007..Additionally, we demonstrated the usefulness of mycorrhizal fungi transformation using Agrobacterium technology to approach gene function...
- Genetic and biochemical studies in yeast reveal that the cotton fibre-specific GhCER6 gene functions in fatty acid elongationYong Mei Qin
National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China
J Exp Bot 58:473-81. 2007..The results suggest that GhCER6 encodes a functional 3-ketoacyl-CoA synthase...
- The NAC domain mediates functional specificity of CUP-SHAPED COTYLEDON proteinsKen Ichiro Taoka
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630 0101, Japan
Plant J 40:462-73. 2004..Taken together, these observations suggest that CUC proteins transactivate the target genes involved in SAM formation and organ separation through a specific interaction between the NAC domain and the promoter region of the target genes...
- [Suppression of nonsense and frameshift mutations obtained by different methods for inactivating the translation termination factor eRF3 in yeast Saccharomyces cerevisiae]S P Zadorskiĭ
Department of Genetics and Breeding, St Petersburg State University, St Petersburg, 199034 Russia
Genetika 39:489-94. 2003....
- One-pot two-step enzymatic coupling of pyrimidine bases to 2-deoxy-D-ribose-5-phosphate. A new strategy in the synthesis of stable isotope labeled deoxynucleosidesN Ouwerkerk
Leiden University, Leiden Institute of Chemistry, Gorlaeus Laboratories, P O Box 9502, 2300 RA Leiden, The Netherlands
J Org Chem 67:1480-9. 2002....
- The effect of polysaccharide-degrading wine yeast transformants on the efficiency of wine processing and wine flavourC Louw
Institute for Wine Biotechnology, Department of Viticulture and Oenology, Stellenbosch University, Matieland Stellenbosch, ZA 7602, South Africa
J Biotechnol 125:447-61. 2006..The Pinot Noir wines were also sensorial evaluated and the tasting panel preferred the wines fermented with the recombinant strains...
- Asilomar's legacy in Aotearoa New ZealandTee Rogers-Hayden
Centre for Environmental Risk, Environmental Sciences, University of East Anglis, Norwich NR4 7TJ, UK
Sci Cult (Lond) 14:393-410. 2005
- Does proline accumulation play an active role in stress-induced growth reduction?Albino Maggio
Center for Plant Environmental Stress Physiology, Purdue University, 1165 Horticulture Bldg, West Lafayette, Indiana 47907 1165, USA
Plant J 31:699-712. 2002..As in previous studies with transgenic plants that overaccumulate osmolytes, we observed some increase in relative growth of proline-overaccumulating cells in mild hyperosmotic stress...
- Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant tryptophan pathwayTakuya Kanno
Cell Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University, Matsuyama 790 8577, Japan
Plant Physiol 138:2260-8. 2005..Thus, our in vitro approach utilizing structural information of bacterial homologs is a potent technique to generate designer enzymes with predefined functions...
- Genetic engineering regulation in Australia: an 'archaeology' of expertise and powerRichard Hindmarsh
Australian School of Environmental Sciences, Griffith University, Nathan, Brisbane, 4111, Australia
Sci Cult (Lond) 14:373-92. 2005
- The science and politics of plant biotechnology--a personal perspectiveIndra K Vasil
University of Florida, Box 110690, Gainesville, Florida 32611 0690, USA
Nat Biotechnol 21:849-51. 2003
- Cloning, expression in yeast, and functional characterization of CYP76A4, a novel cytochrome P450 of petunia that catalyzes (omega-1)-hydroxylation of lauric acidKatsutomo Tamaki
Laboratory of Biotechnology, Hyogo Prefectural Research Institute for Agriculture, Kasai shi, Hyogo, Japan
Biosci Biotechnol Biochem 69:406-9. 2005..The CYP76A4 cDNA was expressed in yeast Saccharomyces cerevisiae AH22. Recombinant yeast microsomes containing the CYP76A4 hemoprotein were found to catalyze (omega-1)-hydroxylation of lauric acid...
- Copper-dependent production of a Pycnoporus coccineus extracellular laccase in Aspergillus oryzae and Saccharomyces cerevisiaeHisashi Hoshida
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube, Japan
Biosci Biotechnol Biochem 69:1090-7. 2005..Our results indicate that a high copper concentration is required for the production of active laccase in heterologous hosts and that the copper is required for a post-transcriptional process...
- Genetically modified organisms in the United States: implementation, concerns, and public perceptionMax P Oeschger
Department of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
Adv Biochem Eng Biotechnol 107:57-68. 2007..These crops, and the other, newer GM crops, have produced no problems and have pioneered a silent agricultural revolution in the USA...
- No Munich on GM cropsPierre Malvoisin
Nat Biotechnol 22:1501; discussion 1501. 2004
- Europe sees sharp decline in GMO researchPeter Mitchell
Nat Biotechnol 21:468-9. 2003
- Concern about Japan's unclear biotech regulationsKazuo N Watanabe
Nature 421:689. 2003
- Biotechnological production and applications of coenzyme Q10Jin Ho Choi
School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul, 151 742, South Korea
Appl Microbiol Biotechnol 68:9-15. 2005..The rational design of metabolic pathways in combination with engineering optimization of fermentation processes could facilitate the development of viable bioconversion processes...
- Genetic engineering: unnatural selectionAllison Snow
Department of Evolution, Ecology and Organismal Biology, Ohio State University, Columbus, Ohio 43210, USA
Nature 424:619. 2003
- Metabolic engineering of ketocarotenoids biosynthesis in the unicelullar microalga Chlamydomonas reinhardtiiRosa León
Departamento de Química y Ciencia de Materiales, Facultad de Ciencias Experimentales, Avda Fuerzas Armadas s n, Universidad de Huelva, 21007 Huelva, Spain
J Biotechnol 130:143-52. 2007..Surprisingly this ketocarotenoid was not astaxanthin nor canthaxanthin. The ketocarotenoid was identified on the basis of its mass spectrum as 3,3'-dihydroxy-beta,epsilon-carotene-4-one (4-keto-lutein) or its isomer ketozeaxanthin...
- Efficient prenylation by a plant geranylgeranyltransferase-I requires a functional CaaL box motif and a proximal polybasic domainD Caldelari
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 3102, USA
Plant Physiol 126:1416-29. 2001....
- Tyr152 plays a central role in the catalysis of 1-aminocyclopropane-1-carboxylate synthaseJian Feng Li
Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, China
J Exp Bot 56:2203-10. 2005..It is therefore concluded that Tyr152, especially its hydroxyl group, plays an essential role in the catalysis of ACC synthase. Thus, a revised catalytic model is hereby proposed for functional ACC synthase...
- EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesisMonica Goicoechea
Institut Fédératif de Recherches FR40, Unité mixte de Recherches 5546, Université Paul Sabatier Centre National de la Recherche Scientifique, Pole de Biotechnologie Vegetale, 24 Chemin de Borde Rouge, BP 17 Auzeville, 31326 Castanet Tolosan, France
Plant J 43:553-67. 2005..Together these results suggest a role for EgMYB2 in the co-ordinated control of genes belonging to the monolignol-specific pathway, and therefore in the biosynthesis of lignin and the regulation of secondary cell wall formation...
- Circadian regulation of the PhCCD1 carotenoid cleavage dioxygenase controls emission of beta-ionone, a fragrance volatile of petunia flowersAndrew J Simkin
Horticultural Sciences, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611 0690, USA
Plant Physiol 136:3504-14. 2004..beta-Ionone emission by flowers occurred principally during daylight hours, paralleling PhCCD1 expression in corollas. The results indicate that PhCCD1 activity and beta-ionone emission are likely regulated at the level of transcript...
- A study in scarlet: enzymes of ketocarotenoid biosynthesis in the flowers of Adonis aestivalisFrancis X Cunningham
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
Plant J 41:478-92. 2005..It is proposed that a 3,4-desaturation subsequent to 4-hydroxylation of the beta-ring leads to the formation of a 4-keto-beta-ring via an indirect and unexpected route: a keto-enol tautomerization...
- Interaction of Arabidopsis BRASSINOSTEROID-INSENSITIVE 1 receptor kinase with a homolog of mammalian TGF-beta receptor interacting proteinHashimul Ehsan
Department of Horticultural Science, North Carolina State University, Raleigh, NC 27695, USA
Plant J 43:251-61. 2005..These findings support a role for TRIP-1 in the molecular mechanisms of BR-regulated plant growth and development, possibly as a cytoplasmic substrate of the BRI1 receptor kinase...
- Contribution of conserved Asn residues to the inhibitory activities of Kunitz-type protease inhibitors from plantsShiroh Iwanaga
Laboratory of Biochemistry, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
Biosci Biotechnol Biochem 69:220-3. 2005..The conserved Asn residues of Kunitz-type inhibitors play an important role in exhibiting effective inhibitory activity by stabilizing the structures of the primary binding loop and protease-inhibitor complex...
- Gene cloning, overproduction, and characterization of thermolabile alkaline phosphatase from a psychrotrophic bacteriumYutaka Suzuki
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Japan
Biosci Biotechnol Biochem 69:364-73. 2005..Comparison of the amino acid sequences of SIB1 and E. coli APases suggests that decreases in electrostatic interactions and number of disulfide bonds are responsible for the cold-adaptation of SIB1 APase...
- Diatom plastids possess a phosphoribulokinase with an altered regulation and no oxidative pentose phosphate pathwayAndreas K Michels
Institute of Plant Biochemistry, University of Dusseldorf, 40225 Duesseldorf, Germany
Plant Physiol 137:911-20. 2005..We discuss our results in the context of rearrangements of the subcellular compartmentation of metabolic pathways due to the peculiar evolution of diatoms by secondary endocytobiosis...
- A polymorphic motif in the small subunit of ADP-glucose pyrophosphorylase modulates interactions between the small and large subunitsJoanna M Cross
Program in Plant Molecular and Cellular Biology and Horticultural Sciences, University of Florida, PO Box 110690, 2211 Fifield Hall, Gainesville, FL 32611, USA
Plant J 41:501-11. 2005..Hence, a small polymorphic motif within the small subunit influences both catalytic and allosteric properties by modulating subunit interactions...
- Analysis of parental strain DNA fragments existing in GEMs-FhhhChun bo Hao
The National Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science, Nanjing University, Nanjing 210093, China
J Environ Sci (China) 15:590-4. 2003....
- Development of a real-time PCR method for the differential detection and quantification of four solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum)Maher Chaouachi
Unité Etude du Polymorphisme des Génomes Végétaux EPGV UR1279, Centre National de Génotypage CNG, 2 rue Gaston Crémieux 91057, CP5721, Evry Cedex, France
J Agric Food Chem 56:1818-28. 2008The labeling of products containing genetically modified organisms (GMO) is linked to their quantification since a threshold for the presence of fortuitous GMOs in food has been established...
- Extracellular plant DNA in Geneva groundwater and traditional artesian drinking water fountainsJohn Pote
Environmental Microbial Genomics Group, Laboratoire AMPERE, Ecole Centrale de Lyon, Universite de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully, France
Chemosphere 75:498-504. 2009..analysis has become central to work on natural gene exchange, forensic analyses, soil bioremediation, genetically modified organisms, exobiology, and palaeontology, fundamental questions about DNA resistance to degradation remain...
- Establishing a risk-assessment process for release of genetically modified wine yeast into the environmentHeidi Schoeman
Institute for Wine Biotechnology, Stellenbosch University, Matieland, ZA, South Africa
Can J Microbiol 55:990-1002. 2009The use and release of genetically modified organisms (GMOs) is an issue of intense public concern and, in the case of food and beverages, products containing GMOs or products thereof carry the risk of consumer rejection...
- [Sustainable production of bulk chemicals by application of "white biotechnology"]M K Patel
Department of Science, Technology and Society, Utrecht University, The Netherlands
Sheng Wu Gong Cheng Xue Bao 24:2022-6. 2008..bacteria and enzymes can be used for White Biotechnology but, according to many experts in the fields, Genetically Modified Organisms (GMO) will be necessary in order to achieve the high yields, concentrations and productivities that ..
- The role of national ethics commissions in FinlandRitva Halila
National Advisory Board on Health Care Ethics, Ministry of Social Affairs and Health, P O Box 33, FIN 00023 Government, Finland
Bioethics 17:357-68. 2003..works as a national authority and gives binding opinions and recommendations about the use of genetically modified organisms. The Sub-Committee on Medical Research Ethics acts a national research ethics committee and gives ..
- Population effects of growth hormone transgenic coho salmon depend on food availability and genotype by environment interactionsRobert H Devlin
Fisheries and Oceans Canada, 4160 Marine Drive, West Vancouver, BC, Canada V7V 1N6
Proc Natl Acad Sci U S A 101:9303-8. 2004Environmental risk assessment of genetically modified organisms requires determination of their fitness and invasiveness relative to conspecifics and other ecosystem members...
- [Genetically modified organisms: a new threat to food safety]Liliane Spendeler
Amigos de la Tierra España, Madrid
Rev Esp Salud Publica 79:271-82. 2005This article analyzes all of the food safety-related aspects related to the use of genetically modified organisms into agriculture and food...
- [Genetically modified organisms--problems and legislation]J Drobnik
Cas Lek Cesk 141:107-11. 2002b>Genetically modified organisms are defined by law as entities capable of replication and/or transmission of hereditary material that had been altered by the insertion or removal of a DNA fragment...
- The dilemma of dual use biological research: Polish perspectiveMarek Czarkowski
Department of Internal Medicine and Endocrinology, Medical University of Warsaw, Banacha 1a, 02 097 Warsaw, Poland
Sci Eng Ethics 16:99-110. 2010..current actions aiming to provide biological safety were based on developing and passing an act about genetically modified organisms (GMO's) and creating a GMO Committee...
- Ethics, evolution and cultureAlex Mesoudi
Centre for Applied Ethics, University of British Columbia, 227 6356 Agricultural Rd, Vancouver V6T 1Z2, BC, Canada
Theory Biosci 127:229-40. 2008..to rapidly changing technological or social environments, such as norms regarding the acceptability of genetically modified organisms. Here we suggest that a science of morality/ethics can benefit from adopting a cultural evolution or ..
- Identification of potentially emerging food safety issues by analysis of reports published by the European Community's Rapid Alert System for Food and Feed (RASFF) during a four-year periodG A Kleter
RIKILT Institute of Food Safety, Wageningen University and Research Center, P O Box 230, NL 6700 AE Wageningen, The Netherlands
Food Chem Toxicol 47:932-50. 2009..Temporary increases were noted in the incidences of the unauthorized dye Para Red, genetically modified organisms, the pesticide isophenfos-methyl, and herring worm, Anisakis simplex...
- Growth of Lactobacillus plantarum in media containing hydrolysates of fish visceraS J Horn
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, As, Norway
J Appl Microbiol 99:1082-9. 2005..To compare growth of Lactobacillus plantarum on media containing hydrolysates (peptones) from cod viscera with growth on commercial media...
- A single mutation in P450BM-3 enhances acyl homoserine lactone: acyl homoserine substrate binding selectivity nearly 250-foldPuneet K Chowdhary
Department of Chemistry, University of Texas at Dallas, Richardson, TX 75080, United States
J Biotechnol 135:374-6. 2008..The enzymatic inactivation of quorum signals has shown promise for use in genetically modified organisms resistant to pathogens...
- Real-time polymerase chain reaction detection of cauliflower mosaic virus to complement the 35S screening assay for genetically modified organismsKatarina Cankar
National Institute of Biology, Department of Plant Physiology and Biotechnology, Vecna pot 111, 1000 Ljubljana, Slovenia
J AOAC Int 88:814-22. 2005Labeling of genetically modified organisms (GMOs) is now in place in many countries, including the European Union, in order to guarantee the consumer's choice between GM and non-GM products...
- Vector-free cloning of a bacterial endo-1,4-beta-glucanase in Lactobacillus plantarum and its effect on the acidifying activity in silage: use of recombinant cellulolytic Lactobacillus plantarum as silage inoculantF Rossi
Dipartimento Scientifico e Tecnologico, Facolta di Scienze MM FF e NN, Universita degli Studi di Verona, Italy
Antonie Van Leeuwenhoek 80:139-47. 2001..Therefore two different genetically modified organisms (GMOs) per each wild-type (WT), producing 43-59 U/l cellulase in 16 h, were examined...
- Democracy and the governance of uncertainty. The case of agricultural gene technologiesL Pellizzoni
Department of Human Sciences, University of Trieste, Piazzale Europa 1, 34127, Trieste, Italy
J Hazard Mater 86:205-22. 2001The use of genetically modified organisms (GMOs) in agriculture and food production is the object of an intense and divisive debate...
- Eradication of a Primary Filariasis Vector Population at an Endemic Field SiteSTEPHEN LEONARD DOBSON; Fiscal Year: 2010..the proposed strategy employs a naturally occurring bacteria infection and does NOT include genetically modified organisms. The preliminary studies section describes how a l/Vo/bac/7/a-infected Ae...
- Biological vector control reducing arboviruses, including Dengue and ChikungunyaJAMES WILLIAM MAINS; Fiscal Year: 2012..albopictus, without involving genetically modified organisms. In brief, the strategy is to release Ae...
- NextGen DNA Binding Proteins Based on TAL DomainsDavid J Segal; Fiscal Year: 2013..of which have had transformative impact in the areas of gene regulation, genome engineering, genetically modified organisms, and gene therapy...
- Reservoirs of Antibiotic Resistance--BioinformaticsMichael Feldgarden; Fiscal Year: 2006..prophylaxis and therapy in human disease, animal husbandry and horticulture and in management of genetically modified organisms. The continued growth of the ROAR network, applied to this question, will thus accelerate the pace of ..
- Metabolomics and metabolic compartmentation in the brainJulian Griffin; Fiscal Year: 2006..To correlate NMR observable metabolite changes with transcriptional changes. Our data will provide proof-of principle for this methodology, and establish a means for exploring other neurolodegenerative disorders. ..
- RADIATION INDUCTION OF GENOMIC REARRANGEMENTS IN YEASTMichael Fasullo; Fiscal Year: 2004..abstract_text> ..
- Activating liver carcinogens in yeast by expressing CYP450 polymorphismsMichael Fasullo; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Targeted Transposons for Gene TherapyPERRY HACKETT; Fiscal Year: 2007..3: Evaluate the efficiency of a LTT targeting tether to enhance uptake and transposition of a pKLAT2 transposon vector into liver cells in mice. [unreadable] [unreadable] [unreadable]..
- Is S-adenosyl Methionine a Nutrition: Genetic Link in AD?Thomas Shea; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Newborn screening for PKU and BH4 responsivenessSteven Dobrowolski; Fiscal Year: 2008..High resolution melt profiling will play a role to identify BH4 responsive PKU patients. ..
- Analysis of Plasmodium development in the mosquitoMarcelo Jacobs Lorena; Fiscal Year: 2006..Knockout mutations will be produced and changes of gene expression caused by these mutations will be measured by use of the micro-arrays. ..
- Species-specific regulated transposition in mosquitoesAnthony James; Fiscal Year: 2008..gambiae and Ae. aegypti. ..
- IMPDH as a Drug Target in CryptosporidiumBoris Striepen; Fiscal Year: 2008..This work will provide urgently needed molecular reagents to understand the biology of nutrient uptake and drug resistance in this unique parasite. [unreadable] [unreadable] [unreadable]..
- LIVER REPOPULATION FOR LIVER INJURY AND GENE THERAPYSanjeev Gupta; Fiscal Year: 2007..Our studies will provide valuable preclinical information for eventual applications of cell therapy in people. ..
- Genetic Analysis of Plasmodium falciparum Gametocyte FormationMarcelo Jacobs Lorena; Fiscal Year: 2007..A better understanding of P. falciparum gametocytogenesis may lead to the development of means to prevent gametocyte formation and thus, block transmission. [unreadable] [unreadable] [unreadable]..
- Ninth International Congress on ToxoplasmosisBoris Striepen; Fiscal Year: 2007..gondii that is not often seen in fields as high paced and[unreadable] competitive and has facilitated rapid and sustained progress in research on this pathogen[unreadable] and related parasites. ..
- Wolbachia as an Agent for Population Replacement in Anopheles GambiaeJason Rasgon; Fiscal Year: 2008..We propose to evaluate transfer Wolbachia infection into Anopheles gambiae and evaluate the symbiont as a candidate transgene drive system for malaria control. [unreadable] [unreadable] [unreadable]..
- Sugar signaling networks detected by high content fluxomicsWolf B Frommer; Fiscal Year: 2010..The networks and databases developed in this project promise to provide novel screening methods for novel drugs as well as help identifying new drug targets. ..
- Evolution of codon usage bias in the genus DrosophilaJeffrey Powell; Fiscal Year: 2009..willistoni, one of the species being sequenced. ..
- Measuring Entomological Risk for DengueTHOMAS WALLACE SCOTT; Fiscal Year: 2010..Our results will be relevant for dengue prevention programs based on vector control and/or vaccine strategies and will have immediate, practical applications for dengue surveillance and control worldwide. ..
- MOLECULAR ANALYSIS OF THE MOSQUITO PERITROPHIC MATRIXMarcelo Jacobs Lorena; Fiscal Year: 2004..gambiae is likely to become available in the near future. The characterization of PM protein genes and an understanding of their function may lead to novel strategies for malaria control. ..
- H. influenzae Genes Associated with Acute Otitis MediaJanet R Gilsdorf; Fiscal Year: 2010....
- Regulation of APP Degradation and ABeta SecretionDavid Schubert; Fiscal Year: 2008..In addition, understanding how cells regulate the degradation of proteins is critical to the study of a wide range of diseases, particularly those in the nervous system that are characterized by the intracellular protein accumulation. ..
- LIVER REPOPULATION FOR LIVER INJURY AND GENE THERAPYSanjeev Gupta; Fiscal Year: 2008..Completion of our studies will generate new knowledge in liver repopulation and cell and gene therapy mechanisms. This will lead to more effective liver-directed cell and gene therapy in people. ..
- Controlled Diversity in Bacterial Stress ResponseAdam Arkin; Fiscal Year: 2009..abstract_text> ..
- Genetic Studies of African Anopheles Vectors of MalariaJeffrey Powell; Fiscal Year: 2009..Replacing natural vector populations with such strains require that the strain have high fitness. ..
- Intermediate Filaments 2008 Gordon Research ConferenceThomas Shea; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Delivery of Sleeping Beauty Transposons to Dog Liver for Gene TherapyPERRY HACKETT; Fiscal Year: 2008..The goal of this project is to develop a transposon delivery system that will allow hydrodynamic delivery of SB transposons in dogs as a model large animal for humans. [unreadable] [unreadable] [unreadable]..
- MOLECULAR AND FUNCTIONAL STUDIES OF H INFLUENZAE PILIJanet Gilsdorf; Fiscal Year: 2001..The receptor binding domain antibodies will be tested for their ability to interfere with the adherence of piliated Hi to human erythrocytes and respiratory cells. ..
- Sterol Biosynthesis in Trypanosomatid ParasitesFrederick Buckner; Fiscal Year: 2004..The drugs discovered in this research program will hopefully provide better future treatment for patients with these devastating diseases. ..
- Nanospheres as Vehicles for Treatment of NeuroblastomaThomas Shea; Fiscal Year: 2004..Resulting data will indicate whether or not nanospheres represent useful delivery agents for cancer therapy. ..
- Protein targeting and secretion in ToxoplasmaBoris Striepen; Fiscal Year: 2004..Mutant analysis will also provide critical information towards the function of the individual secretory organelles involved. ..
- The two-edged sword: vaccines as weaponsJanet Gilsdorf; Fiscal Year: 2003....
- MULTIPLE BLOOD FEEDING BY MOSQUITOESThomas Scott; Fiscal Year: 2003..Insecticide treatments at the end of each experiment will kill any surviving released mosquitoes before they could become infective and transmit virus. ..
- ADAPTING NAGCAT FOR ETHNIC COMMUNITIES : A RESEARCH MODEJOHN SHUTSKE; Fiscal Year: 2002....
- ENTOMOLOGICAL ASSUMPTIONS OF DENGUE CONTROLThomas Scott; Fiscal Year: 2002....
- RECOMBINANT ANTIBODIES BLOCKING SPOROZOITE TRANSMISSIONAnthony James; Fiscal Year: 2002..The idea is that results from these research projects will be relatively easily transferred to the P. falciparum sporozoites and their vectors, anopholine mosquitoes. ..
- PHOSPHATE CONTROL OF THE ESCHERICHIA COLI PHO REGULONBarry Wanner; Fiscal Year: 2001..Common to the three aims is the idea that PhoB, PhoR and PhoU interact. ..
- Structure Function in Low Resistance JunctionsCamillo Peracchia; Fiscal Year: 2005..The relevance of a potential CaM binding site identified in connexins (similar to that of CaM kinase II) will be tested with deletion and mutation of crucial Cx32 residues. ..
- INTERFERON-B AND COPOLYMER-I IN MULTIPLE SCLEROSIS.Suhayl Dhib Jalbut; Fiscal Year: 2003..The proposed research could help identify components of the immunologic network in MS that are associated with response to treatment, and therefore help in the design of more effective future therapies. ..
- Comparative Modeling of Neurodegenerative DiseasesCHRISTOPHER LINK; Fiscal Year: 2006..These studies will directly test whether there is a common underlying toxic mechanism for these neurodegenerative diseases. [unreadable] [unreadable]..
- Genomic-driven Approaches in E.coli PhysiologyBarry Wanner; Fiscal Year: 2005....
- EXPRESSION OF EXOGENOUS GENES IN VECTOR MOSQUITOESAnthony James; Fiscal Year: 2006..stephensi and An. gambiae expressing reporter genes under the control of promoters derived from genes expressed specifically in the adult female fat body cells. ..
- H. influenzae genes associated with COPDJanet Gilsdorf; Fiscal Year: 2009..These results will lead to a better understanding of the H. influenzae virulence pathways associated with COPD and lead to novel new strategies for prevention and management of COPD. ..
- Sensing Biowarfare Agents by Surface Enhanced RamanDonald Moir; Fiscal Year: 2005....
- GENERATION OF A DELETION PANEL FOR THE ZEBRAFISH GENOMEMarnie Halpern; Fiscal Year: 2001..As in non-vertebrate genetic systems, a zebrafish deletion panel is an essential resource, providing the necessary bridge between DNA sequence and gene function. ..
- A Novel Family of Neuroprotective Compounds for StrokeDAVID R SCHUBERT; Fiscal Year: 2010..abstract_text> ..
- Type III Secretion Inhibitors for Anti-Infective TherapyDonald Moir; Fiscal Year: 2007..aeruginosa TTSS; (3) Screen a diverse compound library to identify and validate TTSS inhibitors; and (4) Prioritize validated screening hits for in vitro potency, mechanism, spectrum, and selectivity. [unreadable] [unreadable]..
- GENETIC AND CHEMICAL APPROACHES TO NOVEL LIPID II BINDING PEPTIDE ANTIBIOTICSXihou Yin; Fiscal Year: 2010..Understanding the molecular basis of enduracidin resistance in the producing organism will help predict how new antibiotics may be affected by existing resistance mechanisms and provide insight into ways that resistance may emerge. ..
- Redox Sensitive Proteins in Aging and NeurodegenerationDavid Schubert; Fiscal Year: 2006..These proteins, in turn, will be used as targets for the identification of potentially therapeutic antioxidants that could be useful in preventing age-and disease-associated damage to the CNS. ..
- Race and Public Communication about Human VariationCeleste Condit; Fiscal Year: 2003..The project will focus on the concerns of African Americans about discrimination and genetics and on attitudes about African Americans and genetics held by European Americans and the general population. ..
- DNA helicase and primase inhibitors for biodefenseDonald Moir; Fiscal Year: 2006..Finally, compounds will be tested for lack of toxicity to mammalian cells in culture, and lack of activity against eukaryotic polymerase alpha and helicase, resulting in a collection of validated hits. ..
- Lay and Expert Models of Gene-Environment InteractionCeleste Condit; Fiscal Year: 2008..This will help recipients to adopt behaviors that will better stave off the risks identified by the tests. [unreadable] [unreadable] [unreadable]..
- BIOCOMPLEXITY:ANALYSIS, DESIGN, EVOLUTION-COMPLEX GENESAdam Arkin; Fiscal Year: 2004..abstract_text> ..
- Genotype Analysis for Diagnosis of Urea Cycle DisordersSteven Dobrowolski; Fiscal Year: 2007..The proposed assay panels provide a means to readily assess gene that are well established to contribute to hyperammonemia. [unreadable] [unreadable] [unreadable]..
- Elucidating the Function of Novel Epigenetic ElementHEATHER TRUE KROB; Fiscal Year: 2005....
- Discovery of B. pseudomallei Therapeutics for BiodefenseDonald Moir; Fiscal Year: 2008..pseudomallei targets. In Phase III, a potent, safe, orally active B. pseudomallei inhibitor will be advanced into IND enabling toxicology and safety pharmacology studies and file an IND. [unreadable] [unreadable] [unreadable]..
- FilmArray-A Closed System for Multi-Pathogen ScreeningSteven Dobrowolski; Fiscal Year: 2005..Pouches are designed to assay for candidate infectious agents consistent with clinical presentation. ..
- Development and Testing of a Novel Neuroprotective DrugDavid Schubert; Fiscal Year: 2006..The results from these studies will determine the therapeutic potential of this novel compound. [unreadable] [unreadable]..
- Analysis of ATP7B in Screening for Wilson DiseaseSteven Dobrowolski; Fiscal Year: 2004..Prospective screening for WD improves efficacy of treatment and quality of life for affected patients. Genotyping is the best option to confirm results based upon ceruloplasmin analysis in asymptomatic patients. ..
- PHYSIOLOGY OF NERVE, GLIAL AND MUSCLE CELL LINESDavid Schubert; Fiscal Year: 2005..This work is directly relevant to CNS trauma and stroke as well as to all chronic degenerative diseases where oxidative stress is a pathological hallmark. ..