Genomes and Genes
Summary: A widely cultivated plant, native to Asia, having succulent, edible leaves eaten as a vegetable. (From American Heritage Dictionary, 1982)
Publications252 found, 100 shown here
- Duplication of AP1 within the Spinacia oleracea L. AP1/FUL clade is followed by rapid amino acid and regulatory evolutionD Noah Sather
Department of Biological Sciences, Wayne State University, Detroit, MI, 48202, USA
Planta 229:507-21. 2009..We report on the isolation of three AP1/FUL genes from cultivated spinach, Spinacia oleracea L...
- Comparative study on the changes in photosynthetic activity of the homoiochlorophyllous desiccation-tolerant Haberlea rhodopensis and desiccation-sensitive spinach leaves during desiccation and rehydrationKatya Georgieva
Acad M Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
Photosynth Res 85:191-203. 2005..These features of Haberlea can explain the fast recovery of its photosynthesis after desiccation, which enable this HDT plant to rapidly take advantage of frequent changes in water availability...
- The effect of arsenic contamination on amino acids metabolism in Spinacia oleracea LMilan Pavlík
Isotope Laboratory, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic
Ecotoxicol Environ Saf 73:1309-13. 2010..05). The changes of its concentrations could be affected by changes of pH in plant cell or induction of alanine aminotransferase by hypoxia...
- N-formylkynurenine as a marker of high light stress in photosynthesisTina M Dreaden
School of Chemistry and Biochemistry and Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
J Biol Chem 286:22632-41. 2011..Reactive oxygen species reactions lead to oxidative damage of the reaction center, D1 protein turnover, and inhibition of electron transfer. Our results are consistent with a role for the CP43 NFK modification in photoinhibition...
- Proteome map of the chloroplast lumen of Arabidopsis thalianaMaria Schubert
Department of Medical Nutrition, Karolinska Institute, Novum, Huddinge SE 14186, Sweden
J Biol Chem 277:8354-65. 2002..As a complement to our experimental investigation, we made a theoretical prediction of the luminal proteins from the whole Arabidopsis genome and estimated that the thylakoid lumen of the chloroplast contains approximately 80 proteins...
- Crystal structure of the non-regulatory A(4 )isoform of spinach chloroplast glyceraldehyde-3-phosphate dehydrogenase complexed with NADPS Fermani
Dipartimento di Chimica G Ciamician, Universita di Bologna, Via Selmi 2, Bologna, 40126, Italia
J Mol Biol 314:527-42. 2001..This bridge is not found in other cytosolic or chloroplast GAPDHs from animals, bacteria, or plants other than spinach...
- Involvement of active oxygen species in degradation of light-harvesting proteins under light stressesLello Zolla
Department of Environmental Science, University of Tuscia, Viterbo, Italy
Biochemistry 41:14391-402. 2002....
- Relationship between activity, D1 loss, and Mn binding in photoinhibition of photosystem IIA Krieger
Section de Bioénergétique CNRS URA 2096, CEA Saclay, Gif sur Yvette, France
Biochemistry 37:16262-9. 1998..It is proposed that Mn binding protects against D1 loss by maintaining a protein structure which is not accessible to cleavage...
- Quality control of photosystem II under light stress - turnover of aggregates of the D1 protein in vivoSatoshi Ohira
Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
Photosynth Res 84:29-33. 2005..Consistent with this notion, singlet oxygen production in thylakoid samples under illumination was shown to be stimulated significantly at higher temperatures...
- Malondialdehyde generated from peroxidized linolenic acid causes protein modification in heat-stressed plantsYasuo Yamauchi
Graduate School of Agricultural Science, Kobe University, Kobe, Japan
Plant Physiol Biochem 46:786-93. 2008..This modification was not observed in linolenic acid-deficient mutants (fad3fad7fad8 triple mutant), suggesting that linolenic acid is a major source of protein modification by MDA in heat-stressed plants...
- Hydrogen sulphide enhances photosynthesis through promoting chloroplast biogenesis, photosynthetic enzyme expression, and thiol redox modification in Spinacia oleracea seedlingsJuan Chen
Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, PR China
J Exp Bot 62:4481-93. 2011..In this report, the role of H(2)S in modulating photosynthesis of Spinacia oleracea seedlings was investigated. The main results are as follows...
- Photoproduction of catalase-insensitive peroxides on the donor side of manganese-depleted photosystem II: evidence with a specific fluorescent probeSergey A Khorobrykh
Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia
Biochemistry 50:10658-65. 2011..These ROOH forms, likely including several species derived from lipid peroxides, may mediate the donor side-induced photoinhibition of PSII via protein modification...
- Characterization of interactions between Escherichia coli O157:H7 with epiphytic bacteria in vitro and on spinach leaf surfacesGabriela Lopez-Velasco
Department of Food Science and Technology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Int J Food Microbiol 153:351-7. 2012....
- Multistate outbreak of Escherichia coli O157:H7 infection associated with consumption of packaged spinach, August-September 2006: the Wisconsin investigationArthur M Wendel
Wisconsin Division of Public Health, Madison, Wisconsin, USA
Clin Infect Dis 48:1079-86. 2009..Escherichia coli O157:H7 infection often causes hemorrhagic colitis and hemolytic uremic syndrome...
- Quality control of photosystem II: reactive oxygen species are responsible for the damage to photosystem II under moderate heat stressAmu Yamashita
Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
J Biol Chem 283:28380-91. 2008..Although occurring in the dark, the damages caused by the moderate heat stress to photosystem II are quite similar to those induced by excessive illumination where reactive oxygen species are involved...
- Formation of radicals from singlet oxygen produced during photoinhibition of isolated light-harvesting proteins of photosystem IISara Rinalducci
Department of Environmental Sciences, University of Tuscia, Largo dell Universita, 01100 Viterbo, Italy
Biochim Biophys Acta 1608:63-73. 2004..Thus, monomerization of antennae as well as their incorporation into the PSII complex seem to represent physiologically protected forms. A comparison is made of the photoinhibition mechanisms of different photosynthetic systems...
- Specific plasma membrane aquaporins of the PIP1 subfamily are expressed in sieve elements and guard cellsLaure C Fraysse
Department of Plant Biochemistry, Lund University, P O Box 124, S 22100 Lund, Sweden
Biol Cell 97:519-34. 2005..As the nomenclature implies, the majority of PIPs have been shown to be localized at the plasma membrane. Recently, two highly abundant plasma membrane aquaporins, SoPIP2;1 and SoPIP1;2, have been purified and structurally characterized...
- Quenching of excited states of chlorophyll molecules in submembrane fractions of Photosystem I by exogenous quinonesSubramanyam Rajagopal
Groupe de Recherche en Energie et Information Biomoléculaires, Universite du Quebec a Trois Rivieres, 3351 Boulevard des Forges, Three Rivers, Quebec, Canada GA9 5H7
Biochim Biophys Acta 1606:147-52. 2003....
- Quantitative analysis of the experimental O-J-I-P chlorophyll fluorescence induction kinetics. Apparent activation energy and origin of each kinetic stepSteve Boisvert
Groupe de Recherche en Biologie Végétale, GRBV, Universite du Quebec a Trois Rivieres, Quebec, Canada
FEBS J 273:4770-7. 2006....
- Extended-spectrum Beta-lactamase gene sequences in gram-negative saprophytes on retail organic and nonorganic spinachEva Raphael
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, 201 Hildebrand Hall, Berkeley, CA 94720 7360, USA
Appl Environ Microbiol 77:1601-7. 2011....
- Modulation of flash-induced photosystem II fluorescence by events occurring at the water oxidizing complexI I Putrenko
Department of Biological Sciences, Brock University, St Catharines, Ontario, Canada
Biochemistry 38:10632-41. 1999....
- Coenzyme site-directed mutants of photosynthetic A4-GAPDH show selectively reduced NADPH-dependent catalysis, similar to regulatory AB-GAPDH inhibited by oxidized thioredoxinFrancesca Sparla
Laboratorio di Fisiologia molecolare delle piante, Dipartimento di Biologia Evoluzionistica Sperimentale, Via Irnerio 42, Universita di Bologna, I 40126 Bologna, Italy
J Mol Biol 340:1025-37. 2004..A model based on this concept might be developed to explain the in vivo light-regulation of the GAPDH...
- Development of a dynamic growth-death model for Escherichia coli O157:H7 in minimally processed leafy green vegetablesRobin C McKellar
AAFC Research Associate, Central Experimental Farm, 960 Carling Ave, Ottawa, Ontario, Canada K1N0C6
Int J Food Microbiol 151:7-14. 2011..By incorporating variability, the resulting model is an improvement over existing ones, and is suitable for QRA applications...
- Behavior of Escherichia coli O157:H7 on damaged leaves of spinach, lettuce, cilantro, and parsley stored at abusive temperaturesRowaida K Khalil
Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt
J Food Prot 73:212-20. 2010..This study provides evidence that the damaged portion of a leafy green is a distinct growth niche that elicits different microbial responses in the various types of leafy greens...
- The structure of the chloroplast F1-ATPase at 3.2 A resolutionG Groth
Heinrich Heine Universitat, Biochemie der Pflanzen, Universitatsstrasse 1, D 40225 Dusseldorf, Germany and EMBL Hamburg, Notkestrasse 85, D 22603 Hamburg, Germany
J Biol Chem 276:1345-52. 2001..The structure probably represents an inactive latent state of the ATPase, which is unique to chloroplast and cyanobacterial enzymes...
- Identification of a mechanism of photoprotective energy dissipation in higher plantsAlexander V Ruban
School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK
Nature 450:575-8. 2007..We suggest that this is the principal mechanism of photoprotection...
- Assay of photoinhibition of photosystem II and protease activityYasusi Yamamoto
Graduate School of Natural Science and Technology, Okayama University, Japan
Methods Mol Biol 274:217-27. 2004..The method to analyze the protease activity in the stroma that recognizes and digests the crosslinked products of the D1 protein generated by the light stress is also described...
- Purification and characterization of Delta(1)-pyrroline-5-carboxylate reductase isoenzymes, indicating differential distribution in spinach (Spinacia oleracea L.) leavesM Murahama
Department of Biology, Faculty of Science, Kanazawa University, Kakuma, Kanazawa, 920 1192, Japan
Plant Cell Physiol 42:742-50. 2001..NAD(P)-5-oxidoreductase), the second enzyme in the proline biosynthetic pathway, was purified from spinach (Spinacia oleracea L.) leaves...
- Singlet oxygen scavenging activity of plastoquinol in photosystem II of higher plants: electron paramagnetic resonance spin-trapping studyDeepak Kumar Yadav
Laboratory of Biophysics, Department of Experimental Physics, Faculty of Science, Palacky University, Tr 17 listopadu 12, Olomouc, Czech Republic
Biochim Biophys Acta 1797:1807-11. 2010..These observations reveal that plastoquinol exogenously added to PQ-depleted PSII membranes serves as efficient scavenger of ¹O₂...
- Iodine uptake by spinach (Spinacia oleracea L.) plants grown in solution culture: effects of iodine species and solution concentrationsY G Zhu
Department of Soil Environmental Sciences, Research Centre for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
Environ Int 29:33-7. 2003..out to investigate the effects of iodine species and solution concentrations on iodine uptake by spinach (Spinacia oleracea L.)...
- The 1.49 A resolution crystal structure of PsbQ from photosystem II of Spinacia oleracea reveals a PPII structure in the N-terminal regionMonica Balsera
Instituto de Recursos Naturales y Agrobiología CSIC, Cordel de Merinas 52, 37008 Salamanca, Spain
J Mol Biol 350:1051-60. 2005..In addition, the PsbQ crystal coordinates two zinc ions, one of them is proposed to have a physiological role in higher plants, on the basis of the full conservation of the ligand protein residues in the sequence subfamily...
- Amino acid residues critical for the specificity for betaine aldehyde of the plant ALDH10 isoenzyme involved in the synthesis of glycine betaineAngel G Díaz-Sánchez
Departamento de Bioquimica, Facultad de Quimica, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, 04510 Mexico Distrito Federal, Mexico
Plant Physiol 158:1570-82. 2012..of ω-primary or ω-quaternary aminoaldehydes, but, intriguingly, only some of them, such as the spinach (Spinacia oleracea) betaine aldehyde dehydrogenase (SoBADH), efficiently oxidize betaine aldehyde (BAL) forming the ..
- Identification of oxidized amino acid residues in the vicinity of the Mn(4)CaO(5) cluster of Photosystem II: implications for the identification of oxygen channels within the PhotosystemLaurie K Frankel
Department of Biological Sciences, Division of Biochemistry and Molecular Biology, Louisiana State University, Baton Rouge, LA 70803, USA
Biochemistry 51:6371-7. 2012..These results are discussed within the context of a number of computational studies which have identified putative oxygen channels within the photosystem...
- Photoconsumption of oxygen in photosystem II preparations under impairment of the water-oxidizing complexS A Khorobrykh
Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia
Biochemistry (Mosc) 67:683-8. 2002....
- Three-dimensional electron cryo-microscopy study of the extrinsic domains of the oxygen-evolving complex of spinach: assignment of the PsbO proteinJon Nield
Wolfson Laboratories, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AY, United Kingdom
J Biol Chem 277:15006-12. 2002..This structural assignment is discussed in terms of the recent x-ray-derived cyanobacterial model of PSII (Zouni, A., Witt, H.-T., Kern, J., Fromme, P., Krauss, N., Saenger, W., and Orth, P. (2001) Nature 409, 739-743)...
- Dynamic Interaction between the D1 protein, CP43 and OEC33 at the lumenal side of photosystem II in spinach chloroplasts: evidence from light-induced cross-Linking of the proteins in the donor-side photoinhibitionTakahiro Henmi
Graduate School of Natural Science and Technology, Okayama University, Okayama, 700 8530 Japan
Plant Cell Physiol 44:451-6. 2003..Acad. Sci. USA, 100: 98]. Moreover, the data suggest that the binding condition and positioning of the OEC33 in the photosystem II complex from higher plants may be different from those in cyanobacteria...
- Enhanced susceptibility of photosynthesis to low-temperature photoinhibition due to interruption of chill-induced increase of S-adenosylmethionine decarboxylase activity in leaves of spinach (Spinacia oleracea L.)Lixiong He
Department of Life Science, Faculty of Bioresources, Mie University, Tsu, 514 8507 Japan
Plant Cell Physiol 43:196-206. 2002The possible involvement of polyamines in the chilling tolerance of spinach (Spinacia oleracea L.) was investigated focusing on photosynthesis...
- Evidence that cytochrome b559 is involved in superoxide production in photosystem II: effect of synthetic short-chain plastoquinones in a cytochrome b559 tobacco mutantPavel Pospisil
Laboratory of Biophysics, Department of Experimental Physics, Faculty of Science, Palacky University, tr Svobody 26, 771 46 Olomouc, Czech Republic
Biochem J 397:321-7. 2006..Based on these findings it is suggested that short-chain PQs stimulate O2*- production via a mechanism that involves electron transfer from Pheo- (pheophytin) to LP cyt b559 and subsequent auto-oxidation of LP cyt b559...
- Structural mechanism of plant aquaporin gatingSusanna Törnroth-Horsefield
Department of Chemistry and Bioscience, Chalmers University of Technology, P O Box 462, SE 40530 Goteborg, Sweden
Nature 439:688-94. 2006..These results reveal a molecular gating mechanism which appears conserved throughout all plant plasma membrane aquaporins...
- Structure and function of the PsbP protein of photosystem II from higher plantsKentaro Ifuku
Graduate School of Biostudies, Kyoto University, Kyoto 606 8502, Japan
Photosynth Res 84:251-5. 2005..The possible N-terminal residues of PsbP that would be involved in this interaction are discussed...
- Quality control of Photosystem II: cleavage and aggregation of heat-damaged D1 protein in spinach thylakoidsKeisuke Komayama
Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
Biochim Biophys Acta 1767:838-46. 2007..The cleavage and aggregation of the D1 protein appear to be two alternative processes influenced by protein phosphorylation/dephosphorylation, distribution of FtsH, and intactness of the thylakoids...
- Mapping of the genes for dioecism and monoecism in Spinacia oleracea L.: evidence that both genes are closely linkedYasuyuki Onodera
Laboratory of Genetic Engineering, Research Faculty of Agriculture, Hokkaido University, N 9, W 9, Sapporo 060 8589, Japan
Plant Cell Rep 30:965-71. 2011..SO4 will enable breeders to efficiently select highly male monoecious plants for preferential use as the pollen parent for hybrid seed production...
- Effect of organic and conventional cropping systems on ascorbic acid, vitamin C, flavonoids, nitrate, and oxalate in 27 varieties of spinach (Spinacia oleracea L.)Eunmi Koh
Department of Food Science and Technology, University of California at Davis, One Shields Avenue, Davis, California 95616, USA
J Agric Food Chem 60:3144-50. 2012..These results suggest that organic cropping systems result in spinach with lower levels of nitrates and higher levels of flavonoids and ascorbic acid...
- Documentation of significant electron transport defects on the reducing side of photosystem II upon removal of the PsbP and PsbQ extrinsic proteinsJohnna L Roose
Department of Biological Sciences, Biochemistry and Molecular Biology Section, Louisiana State University, Baton Rouge, Louisiana 70803, USA
Biochemistry 49:36-41. 2010..6 M urea did not induce further reducing-side alterations. Our results demonstrate that studies attributing defects observed upon PsbP and PsbQ removal solely to the oxidizing side must be viewed with caution...
- Spinacia oleracea L. leaf stomata harboring Cryptosporidium parvum oocysts: a potential threat to food safetyDumitru Macarisin
United States Department of Agriculture Agricultural Research Service USDA ARS, Beltsville Agricultural Research Center BARC, Environmental Microbial and Food Safety Laboratory, 10300 Baltimore Avenue, Bldg 173, BARC East, Beltsville, MD 20705, USA
Appl Environ Microbiol 76:555-9. 2010..These findings and the fact that this pathogenic parasite resists washing and disinfection raise concerns regarding food safety...
- Valinomycin sensitivity proves that light-induced thylakoid voltages result in millisecond phase of chlorophyll fluorescence transientsPavel Pospisil
FB Physik, Freie Universitat Berlin, Arminallee 14, D 14195 Dahlem, Berlin, Germany
Biochim Biophys Acta 1554:94-100. 2002..A putative role of thylakoid voltages in protection of PSII against photoinhibitory damage is discussed...
- Chlorophyll thermoluminescence of leaf discs: simple instruments and progress in signal interpretation open the way to new ecophysiological indicatorsJean Marc Ducruet
Service de Bioenergetique, Bat 532, INRA CEA Saclay, 91191 Gif sur Yvette Cedex, France
J Exp Bot 54:2419-30. 2003....
- Structure of the c14 rotor ring of the proton translocating chloroplast ATP synthaseMelanie Vollmar
Institut für Biochemie der Pflanzen, Heinrich Heine Universitat Dusseldorf, 40225 Dusseldorf, Germany
J Biol Chem 284:18228-35. 2009..Reprotonation of Glu(61) by a conserved arginine in the adjacent a subunit returns the carboxylate to its initial conformation...
- Solution structures of spinach acyl carrier protein with decanoate and stearateGregory A Zornetzer
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706 1549, USA
Biochemistry 45:5217-27. 2006..These structures demonstrate the conformational flexibility of spinach ACP and suggest how the protein changes to accommodate its myriad binding partners...
- The 5A structure of heterologously expressed plant aquaporin SoPIP2;1W Kukulski
Maurice E Müller Institute for Microscopy, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
J Mol Biol 350:611-6. 2005..This crystal packing arrangement provides access to the phosphorylated C terminus as well as the loop B phosphorylation site for studies of channel gating...
- Flippase activity in proteoliposomes reconstituted with Spinacea oleracea endoplasmic reticulum membrane proteins: evidence of biogenic membrane flippase in plantsSantosh Kumar Sahu
Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India
Biochemistry 47:10481-90. 2008..These results suggest the existence of biogenic membrane flippases in plants and that the mechanism of membrane biogenesis is similar to that found in animals...
- Structural and functional self-organization of Photosystem II in grana thylakoidsHelmut Kirchhoff
Institut fur Botanik, Schlossgarten 3, D 48149 Munster, Germany
Biochim Biophys Acta 1767:1180-8. 2007..This indicates that the supramolecular protein arrangement in grana thylakoids is a self-organized process...
- Spinacia oleracea L. protects against gamma radiations: a study on glutathione and lipid peroxidation in mouse liverA L Bhatia
Department of Zoology, Radiation Biology Laboratory, University of Rajasthan, Jaipur, India
Phytomedicine 11:607-15. 2004The present study deals with the protective effect of Spinacia oleracea L. against radiation-induced oxidative stress, which is evaluated in terms of lipid peroxidation (LPO) product and tissue levels of glutathione...
- Interaction of Escherichia coli with growing salad spinach plantsKeith Warriner
Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK
J Food Prot 66:1790-7. 2003..coli. The implications of this study's findings with regard to the microbiological safety of minimally processed vegetables are discussed...
- The nuclear RPL4 gene encodes a chloroplast protein that co-purifies with the T7-like transcription complex as well as plastid ribosomesY Trifa
Laboratoire de Génétique Moléculaire des Plantes, Université Joseph Fourier and Centre National de la Recherche Scientifique, B P 53, F 38041 Grenoble, France
J Biol Chem 273:3980-5. 1998....
- Lipids in oxygen-evolving photosystem II complexes of cyanobacteria and higher plantsIsamu Sakurai
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902
J Biochem 140:201-9. 2006..These findings suggest that phosphatidylglycerol plays important roles in the electron transport at the QB-binding site in photosystem II complexes...
- Trypsin inhibitors from the garden four o'clock (Mirabilis jalapa) and spinach (Spinacia oleracea) seeds: isolation, characterization and chemical synthesisJolanta Kowalska
Faculty of Biotechnology, University of Wrocław, Tamka 2, 50 137 Wrocław, Poland
Phytochemistry 68:1487-96. 2007Five serine proteinase inhibitors (Mirabilis jalapa trypsin inhibitors, MJTI I and II and Spinacia oleracea trypsin inhibitors, SOTI I, II, and III) from the garden four-o'clock (M. jalapa) and spinach (S. oleracea) seeds were isolated...
- Differences in the metabolite profiles of spinach (Spinacia oleracea L.) leaf in different concentrations of nitrate in the culture solutionKeiki Okazaki
National Agricultural Research Center for Hokkaido Region, Sapporo 062 8555, Japan
Plant Cell Physiol 49:170-7. 2008....
- Probing the organization of photosystem II in photosynthetic membranes by atomic force microscopyHelmut Kirchhoff
Institut fur Botanik, Schlossgarten 3, D 48149 Munster, Germany
Biochemistry 47:431-40. 2008..The functional consequences for lateral migration processes are discussed...
- Arrangement of photosystem II supercomplexes in crystalline macrodomains within the thylakoid membrane of green plant chloroplastsE J Boekema
Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, 9747 AG, The Netherlands
J Mol Biol 301:1123-33. 2000..The implications of this type of organization for the efficient transfer of excitation energy from LHCII to PSII and for the stacking of grana membranes are discussed...
- Sequence evolution and sex-specific expression patterns of the C class floral identity gene, SpAGAMOUS, in dioecious Spinacia oleracea LD Noah Sather
Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
Planta 222:284-92. 2005Development in dioecious cultivated spinach, Spinacia oleracea, is distinguished by the absence of alternative reproductive organ primordia in male and female flowers...
- Re-consideration of Peronospora farinosa infecting Spinacia oleracea as distinct species, Peronospora effusaYoung Joon Choi
Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul 136 701, Korea
Mycol Res 111:381-91. 2007Downy mildew is probably the most widespread and potentially destructive global disease of spinach (Spinacia oleracea)...
- CPD photolyase gene from Spinacia oleracea: repair of UV-damaged DNA and expression in plant organsRyouhei Yoshihara
The United Graduate School of Agricultural Sciences, Tottori University, Japan
J Radiat Res 46:157-64. 2005..In both the male and female plants, the gene was highly expressed in leaves and flowers under the condition of 14-h light and 10-h dark cycle. The expression in the roots was quite low compared with the other organs...
- Characterization of SpAPETALA3 and SpPISTILLATA, B class floral identity genes in Spinacia oleracea, and their relationship to sexual dimorphismCatherine Pfent
School of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA
Dev Genes Evol 215:132-42. 2005..These observations suggest that regulation of B class floral identity genes has a role in the development of sexual dimorphism and dioecy in spinach rather than being a secondary result of organ abortion...
- Localization of the 23-kDa subunit of the oxygen-evolving complex of photosystem II by electron microscopyE J Boekema
Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
Eur J Biochem 252:268-76. 1998..5 nm. Measured from the centres of the masses, the two 33-kDa subunits associated with the dimeric PSII-LHCII SC are separated by 6.3 nm. The corresponding distance between the two 23-kDa subunits is 8.8 nm...
- Reconstitution of water channel function of an aquaporin overexpressed and purified from Pichia pastorisMaria Karlsson
Department of Plant Biochemistry, Lund University, Box 124, Sweden
FEBS Lett 537:68-72. 2003..PM28A was efficiently solubilized from urea- and alkali-stripped Pichia membranes by octyl-beta-D-thioglucopyranoside resulting in a final yield of 25 mg of purified protein per liter of cell culture...
- CNS depressive role of aqueous extract of Spinacia oleracea L. leaves in adult male albino ratsSutapa Das
S N Pradhan Centre for Neursciences, University of Calcutta, Kolkata, India
Indian J Exp Biol 46:185-90. 2008Treatment with Spinacia oleracea extract (SO; 400 mg/kg body weight) decreased the locomotor activity, grip strength, increased pentobarbitone induced sleeping time and also markedly altered pentylenetetrazole induced seizure status in ..
- The plastid chromosome of spinach (Spinacia oleracea): complete nucleotide sequence and gene organizationC Schmitz-Linneweber
, , Germany
Plant Mol Biol 45:307-15. 2001The chloroplast chromosome of spinach (Spinacia oleracea) is a double-stranded circular DNA molecule of 150,725 nucleotide pairs...
- Arrangement of photosystem II and ATP synthase in chloroplast membranes of spinach and peaBertram Daum
Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany
Plant Cell 22:1299-312. 2010..Grana formation is likely to result from electrostatic interactions between these complexes across the stromal gap...
- Interactions between Spinacia oleracea and Bradysia impatiens: a role for phytoecdysteroidsEric A Schmelz
Department of Entomology, University of Arizona, Tucson, USA
Arch Insect Biochem Physiol 51:204-21. 2002..In spinach (Spinacia oleracea), 20-hydroxyecdysone (20E) concentrations in the roots rapidly increase following root damage, root ..
- Low-light-induced formation of semicrystalline photosystem II arrays in higher plant chloroplastsHelmut Kirchhoff
Institute of Botany, Schlossgarten 3, D 48149 Munster, Germany
Biochemistry 46:11169-76. 2007..Furthermore, the occurrence of a hexagonal phase of the lipid monogalactosyldiacylglycerol in grana membranes of low-light-adapted plants could trigger the rearrangement by changing the lateral membrane pressure...
- Recirculating immunomagnetic separation and optimal enrichment conditions for enhanced detection and recovery of low levels of Escherichia coli O157:H7 from fresh leafy produce and surface waterSunee Himathongkham
Food and Drug Laboratory Branch, California Department of Public Health, Richmond, California 94804, USA
J Food Prot 70:2717-24. 2007..The method used in this study was effective for monitoring E. coli O157:H7 in the farm environment, during postharvest processing, and in foodborne outbreak investigations...
- Involvement of uncoupled antenna chlorophylls in photoinhibition in thylakoidsS Santabarbara
Centro C.N.R. Biologia Cellulare e Molecolare delle Piante, Dipartamento de Biologia, , Via Celoria 26, 20133 Milan, Italy
FEBS Lett 491:109-13. 2001..The broadness of the action spectrum suggests that degraded or incompletely assembled pigment-protein complexes may be involved...
- Pathways and timescales of primary charge separation in the photosystem II reaction center as revealed by a simultaneous fit of time-resolved fluorescence and transient absorptionVladimir I Novoderezhkin
A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow, Russia
Biophys J 89:1464-81. 2005....
- Thioredoxin target proteins in chloroplast thylakoid membranesYves Balmer
Department of Plant and Microbial Biology, University of California, Berkeley, 94720, USA
Antioxid Redox Signal 8:1829-34. 2006....
- Carotenoid specificity of light-harvesting complex II binding sites. Occurrence of 9-cis-violaxanthin in the neoxanthin-binding site in the parasitic angiosperm Cuscuta reflexaAlison M Snyder
School of Biological Sciences, University of Canterbury, Christchurch 8020, New Zealand
J Biol Chem 279:5162-8. 2004..These results suggest that the N1 binding site of LHCIIb preferentially binds 9-cis-5,6-epoxy carotenoids, which has implications for the features of this binding site and its role in the photosystem II antenna assembly and stability...
- Effects of acid and alkali on the light absorption, energy transfer and protein secondary structures of core antenna subunits CP43 and CP47 of photosystem IIShukui Guo
Key Lab of Photosynthesis and Environment Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, People s Republic of China
Photochem Photobiol 79:291-6. 2004....
- Perturbation of the structure of P680 and the charge distribution on its radical cation in isolated reaction center complexes of photosystem II as revealed by fourier transform infrared spectroscopyTatsunori Okubo
Institute of Materials Science, University of Tsukuba, Tsukuba, Ibaraki 305 8573, Japan
Biochemistry 46:4390-7. 2007..This potential decrease explains the well-known phenomenon that YZ is not oxidized by P680+ in RC complexes...
- Effects of nano-anatase on spectral characteristics and distribution of LHCII on the thylakoid membranes of spinachLei Zheng
College of Life Sciences, Suzhou University, Suzhou 215123, People s Republic of China
Biol Trace Elem Res 120:273-83. 2007....
- The stoichiometry of the two photosystems in higher plants revisitedDa Yong Fan
Photobioenergetics Group, Research School of Biological Sciences, The Australian National University, Canberra, ACT 0200, Australia
Biochim Biophys Acta 1767:1064-72. 2007....
- Multidimensional proteomic analysis of photosynthetic membrane proteins by liquid extraction-ultracentrifugation-liquid chromatography-mass spectrometryChristian G Huber
Department of Chemistry, Instrumental Analysis and Bioanalysis, Saarland University, Saarbrucken, Germany
Proteomics 4:3909-20. 2004..Because of its high flexibility and suitability for proteins having a very wide range of Mr and hydrophobicities, the method is generally applicable to the analysis of complex mixtures of membrane proteins...
- Light absorption by the chlorophyll a-b complexes of photosystem II in a leaf with special reference to LHCIIAndrea Rivadossi
Istituto di Biofisica del Consiglio Nazionale delle Ricerche Sezione di Milano, Dipartimento di Biologia, Universita degli Studi di Milano, Milano, Italy
Photochem Photobiol 80:492-8. 2004..The relative enhancement of the chl b absorption is principally associated with the Qy electronic transition region, the light-harvesting contribution of which becomes prominent in the leaf...
- Investigation of the lateral light-induced migration of photosystem II light-harvesting proteins by nano-high performance liquid chromatography electrospray ionization mass spectrometryAnna M Timperio
Department of Environmental Science, University of Tuscia, 01100 Viterbo, Italy
J Biol Chem 280:28858-66. 2005..We hypothesize that the Lhcb1 isoforms expressed by the multigene families play a role in plant adaptation...
- Diffusion of light-harvesting complex II in the thylakoid membranesElena Consoli
Department of Biology and Interdisciplinary Center for Nanostructured Materials and Interfaces CIMAINA, University of Milan, Italy
EMBO Rep 6:782-6. 2005..We found that the population of P-LHCII in unappressed membranes is more mobile than the population of non-P-LHCII from the same regions...
- Carotenoid cation formation and the regulation of photosynthetic light harvestingNancy E Holt
Department of Chemistry, University of California, Berkeley, CA 94720, USA
Science 307:433-6. 2005..These results indicate that energy transfer from chlorophyll molecules to a chlorophyllzeaxanthin heterodimer, which then undergoes charge separation, is the mechanism for excess energy dissipation during feedback deexcitation...
- The glutathione peroxidase homologous gene Gpxh in Chlamydomonas reinhardtii is upregulated by singlet oxygen produced in photosystem IIBeat B Fischer
Department of Environmental Toxicology, Swiss Federal Institute for Environmental Science and Technology EAWAG, Uberlandstr 133, Dubendorf 8600, Switzerland
Planta 223:583-90. 2006..This indicates that the Gpxh expression is induced by 1O2 under environment conditions causing photoinhibition...
- The mobile thylakoid phosphoprotein TSP9 interacts with the light-harvesting complex II and the peripheries of both photosystemsMaria Hansson
Division of Cell Biology, Linkoping University, SE 581 85 Linkoping, Sweden
J Biol Chem 282:16214-22. 2007..An association of TSP9 with LHCII as well as the peripheries of the photosystems suggests its involvement in regulation of photosynthetic light harvesting...
- Rapid loss of structural motifs in the manganese complex of oxygenic photosynthesis by X-ray irradiation at 10-300 KMarkus Grabolle
Freie Universitat Berlin, FB Physik, Arnimallee 14, D 14195 Berlin, Germany
J Biol Chem 281:4580-8. 2006..It is proposed to employ controlled x-ray photoreduction in metalloprotein research for: (i) population of distinct reduced states, (ii) estimating the redox potential of buried metal centers, and (iii) research on protein dynamics...
- Dimeric and monomeric organization of photosystem II. Distribution of five distinct complexes in the different domains of the thylakoid membraneRavi Danielsson
Department of Biochemistry, Center for Chemistry and Chemical Engineering, P O Box 124, Lund University, S 221 00 Lund, Sweden
J Biol Chem 281:14241-9. 2006..PSII core monomers were heterogeneous, and a large fraction did not evolve oxygen. PSII monomers without the CP43 protein and PSII reaction centers showed no oxygen-evolving activity...
- Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macro-assemblies with light-inducible structural transitionsIlian Simidjiev
Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Szeged
Methods Mol Biol 274:105-14. 2004....
- Biochemical properties of the PsbS subunit of photosystem II either purified from chloroplast or recombinantPaola Dominici
Dipartimento Scientifico e Tecnologico, Universita degli Studi di Verona, 37134 Verona, Italy
J Biol Chem 277:22750-8. 2002..This domain is suggested to be involved in sensing low luminal pH...
- In vitro reconstitution of the activated zeaxanthin state associated with energy dissipation in plantsMark Aspinall-O'Dea
Robert Hill Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
Proc Natl Acad Sci U S A 99:16331-5. 2002..The altered properties of zeaxanthin and PsbS that result from this interaction provide the first direct indication about how they regulate energy dissipation...
- Polyphenolic allelochemicals from the aquatic angiosperm Myriophyllum spicatum inhibit photosystem IIEva Leu
Limnologiesches Institut, Universitat Konstanz, Freiburg, Germany
Plant Physiol 130:2011-8. 2002..Separate measurements of photosystem I and II activity with spinach (Spinacia oleracea) thylakoid membranes indicated that the site of inhibition is located at photosystem II (PSII)...
- Singlet oxygen production in herbicide-treated photosystem IIChristian Fufezan
Institut fur Biologie II, Biochemie der Pflanzen, Universitat Freiburg, Schänzlestr 1, 79104, Freiburg, Germany
FEBS Lett 532:407-10. 2002..When phenolic herbicides are bound, charge recombination at the level of P+*Pheo-* is thermodynamically favoured forming a chlorophyll triplet and hence 1O2. With urea herbicides this pathway is less favourable...
- Light-induced trimer to monomer transition in the main light-harvesting antenna complex of plants: thermo-optic mechanismGyozo Garab
Institutes of Plant Biology and Biophysics, Biological Research Center, Hungarian Academy of Sciences, P O Box 521, H 6701 Szeged, Hungary
Biochemistry 41:15121-9. 2002..Structural changes driven by thermo-optic mechanisms may therefore provide plants with a novel mechanism for regulation of light harvesting in excess light...
- Energy dissipation in photosynthesis: does the quenching of chlorophyll fluorescence originate from antenna complexes of photosystem II or from the reaction center?N G Bukhov
Julius von Sachs Institut fur Biowissenschaften, Universitat Wurzburg, 97082 Wurzburg, Germany
Planta 212:749-58. 2001..Warnst., and in leaves of Spinacia oleracea L. and Arabidopsis thaliana (L.) Heynh., using chlorophyll fluorescence as an indicator reaction...
- A few molecules of zeaxanthin per reaction centre of photosystem II permit effective thermal dissipation of light energy in photosystem II of a poikilohydric mossN G Bukhov
, , , Germany
Planta 212:739-48. 2001..20% CO2 in air was incapable of quenching basal, so-called F0 fluorescence either in the moss or in spinach (Spinacia oleracea L.) leaves...
- Energy transfer in light-harvesting complexes LHCII and CP29 of spinach studied with three pulse echo peak shift and transient gratingJante M Salverda
Department of Biophysics and Physics of Complex Systems, Division of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit Amsterdam, The Netherlands
Biophys J 84:450-65. 2003..Our study is the first to provide spectroscopic evidence for mixed binding sites, as well as the first to show their existence in native complexes...
- Oxidation of the two beta-carotene molecules in the photosystem II reaction centerAlison Telfer
Wolfson Laboratories, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK
Biochemistry 42:1008-15. 2003..Although contributions of chlorophyll cations cannot be formally ruled out, our results demonstrate that these spectra mainly arise from the cation radical species of the two carotenoids present in photosystem II reaction centers...
- Active oxygen produced during selective excitation of photosystem I is damaging not only to photosystem I, but also to photosystem IIS E Tjus
Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark
Plant Physiol 125:2007-15. 2001..aim to specifically study the molecular mechanisms behind photoinhibition of photosystem I, stacked spinach (Spinacia oleracea) thylakoids were irradiated at 4 degrees C with far-red light (>715 nm) exciting photosystem I, but not ..
- Posttranslational modifications in the CP43 subunit of photosystem IILorraine B Anderson
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, St Paul 55108, USA
Proc Natl Acad Sci U S A 99:14676-81. 2002..As a step toward understanding the tertiary structure of this CP43 peptide, structural modeling was performed by using molecular dynamics...
- Retinol Equivalence of Plant Carotenoids in ChildrenGuangwen Tang; Fiscal Year: 2006..This study will be of importance in planning vitamin A deficiency prevention strategies and also will provide useful information regarding the potential efficacy of a bioengineered crop to provide vitamin A nutrition. ..
- Thermodynamic Properties of NADH:Nitrate ReductaseMichael Barber; Fiscal Year: 2005..These studies will enhance our understanding of important structure-function relationships in NR and other related metalloflavoproteins. ..
- SPECTROSCOPIC STUDIES OF LIGHT-DRIVEN ELECTRON TRANSFERBRIDGETTE ANNE BARRY; Fiscal Year: 2010..This specific aim will serve to elucidate the role of primary, secondary, and tertiary interactions in a structurally defined, tractable model system. ..
- Therapeutic vaccine against tuberculosisLee Riley; Fiscal Year: 2006..Therapeutic vaccines against TB is a major priority in TB control, and we have an opportunity to identify such products by a novel approach using a simple, inexpensive, and reproducible animal model. ..
- ANTIOXIDANT GENES OF MYCOBACERIUM TUBERCULOSISLee Riley; Fiscal Year: 2001....
- Expression of Human Therapeutic Proteins in Transgenic Tobacco ChloroplastsHenry Daniell; Fiscal Year: 2010..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
- MOLECULAR BIOLOGY OF INFECTION WITH M TUBERCULOSISLee Riley; Fiscal Year: 2002..In addition, these studies may identify several potential targets for new chemotherapeutic agents or vaccine candidates. ..
- STRUCTURE AND FUNCTION OF CAROTENOIDSHarry Frank; Fiscal Year: 2008..Several preliminary studies have already been carried out by us in support of the methods we propose to employ. ..
- In vivo regulation of M. tuberculosis cell wall lipidsLee W Riley; Fiscal Year: 2010..tuberculosis and granulomas may contribute to our understanding of the mechanism of persistence of M. tuberculosis that could lead to new tests to differentiate active TB from latent TB infection. ..
- Orgin of multidrug resistant uropathogenic E. coliLee Riley; Fiscal Year: 2008..This knowledge should lead to the implementation of improved control of drug-resistant infectious diseases. ..
- ALCOHOL EFFECT ON HUMAN LACTATION AND INFANT BEHAVIORJULIE MENNELLA; Fiscal Year: 2007..AIM 3: To explore the role of early experiences with ethanol in mothers' milk and the emotional context of such experiences, on the recognition of, and preference for, the sensory properties of ethanol ..
- REDOX PROPERTIES OF FLAVODOXIN-LIKE FLAVOPROTEINSRICHARD SWENSON; Fiscal Year: 2007..These studies are also designed to expand our understanding of the fundamentally different way that the one electron reduced state (the flavin semiquinone) is stabilized and is utilized in these two different systems. ..
- Epidemiology of an uropathogenic E coli clonal groupLee Riley; Fiscal Year: 2003..coli can be spread by contaminated ingestible vehicles, and use the data generated from this project to design long-term studies to better characterize risk factors associated with CgA infection. ..
- Flow Cytometry to Detect Human Tumor Cells in Body FluidAWTAR GANJU KRISHAN; Fiscal Year: 2003..3. Compare data collected from flow cytometric analysis with that obtained from cytopathology and immunocytochemistry routinely performed on these specimens for diagnostic purpose. ..
- F1-ATPase Chemical-Mechanical Coupling MechanismsWayne D Frasch; Fiscal Year: 2010..abstract_text> ..
- NOVEL E. COLI 0157:H7 INTESTINAL COLONIZATION FACTORSJAMES KAPER; Fiscal Year: 2009....
- DEVELOPMENT OF A LIVE ORAL CHOLERA VACCINEJames B Kaper; Fiscal Year: 2010..Lay summary: Vibrio cholerae is a bacterium that causes cholera, a severe diarrheal disease that can often cause death. This project will develop improved vaccines for the prevention of cholera. ..
- MOLECULAR GENETICS OF ENTEROPATHOGENIC E COLI ADHESIONJAMES KAPER; Fiscal Year: 2003..The proposed experimental approach will use a combination of molecular genetics, cell biology, and animal studies to achieve a better understanding of how EPEC infects intestinal epithelial cells and causes disease. ..