spinacia oleracea

Summary

Summary: A widely cultivated plant, native to Asia, having succulent, edible leaves eaten as a vegetable. (From American Heritage Dictionary, 1982)

Top Publications

  1. ncbi Duplication of AP1 within the Spinacia oleracea L. AP1/FUL clade is followed by rapid amino acid and regulatory evolution
    D Noah Sather
    Department of Biological Sciences, Wayne State University, Detroit, MI, 48202, USA
    Planta 229:507-21. 2009
  2. ncbi Comparative study on the changes in photosynthetic activity of the homoiochlorophyllous desiccation-tolerant Haberlea rhodopensis and desiccation-sensitive spinach leaves during desiccation and rehydration
    Katya Georgieva
    Acad M Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
    Photosynth Res 85:191-203. 2005
  3. ncbi The effect of arsenic contamination on amino acids metabolism in Spinacia oleracea L
    Milan Pavlík
    Isotope Laboratory, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic
    Ecotoxicol Environ Saf 73:1309-13. 2010
  4. pmc N-formylkynurenine as a marker of high light stress in photosynthesis
    Tina M Dreaden
    School of Chemistry and Biochemistry and Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    J Biol Chem 286:22632-41. 2011
  5. ncbi Proteome map of the chloroplast lumen of Arabidopsis thaliana
    Maria Schubert
    Department of Medical Nutrition, Karolinska Institute, Novum, Huddinge SE 14186, Sweden
    J Biol Chem 277:8354-65. 2002
  6. ncbi Crystal structure of the non-regulatory A(4 )isoform of spinach chloroplast glyceraldehyde-3-phosphate dehydrogenase complexed with NADP
    S Fermani
    Dipartimento di Chimica G Ciamician, Universita di Bologna, Via Selmi 2, Bologna, 40126, Italia
    J Mol Biol 314:527-42. 2001
  7. ncbi Involvement of active oxygen species in degradation of light-harvesting proteins under light stresses
    Lello Zolla
    Department of Environmental Science, University of Tuscia, Viterbo, Italy
    Biochemistry 41:14391-402. 2002
  8. ncbi Relationship between activity, D1 loss, and Mn binding in photoinhibition of photosystem II
    A Krieger
    Section de Bioénergétique CNRS URA 2096, CEA Saclay, Gif sur Yvette, France
    Biochemistry 37:16262-9. 1998
  9. ncbi Quality control of photosystem II under light stress - turnover of aggregates of the D1 protein in vivo
    Satoshi Ohira
    Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Photosynth Res 84:29-33. 2005
  10. ncbi Malondialdehyde generated from peroxidized linolenic acid causes protein modification in heat-stressed plants
    Yasuo Yamauchi
    Graduate School of Agricultural Science, Kobe University, Kobe, Japan
    Plant Physiol Biochem 46:786-93. 2008

Detail Information

Publications252 found, 100 shown here

  1. ncbi Duplication of AP1 within the Spinacia oleracea L. AP1/FUL clade is followed by rapid amino acid and regulatory evolution
    D Noah Sather
    Department of Biological Sciences, Wayne State University, Detroit, MI, 48202, USA
    Planta 229:507-21. 2009
    ..We report on the isolation of three AP1/FUL genes from cultivated spinach, Spinacia oleracea L...
  2. ncbi Comparative study on the changes in photosynthetic activity of the homoiochlorophyllous desiccation-tolerant Haberlea rhodopensis and desiccation-sensitive spinach leaves during desiccation and rehydration
    Katya Georgieva
    Acad M Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
    Photosynth Res 85:191-203. 2005
    ..These features of Haberlea can explain the fast recovery of its photosynthesis after desiccation, which enable this HDT plant to rapidly take advantage of frequent changes in water availability...
  3. ncbi The effect of arsenic contamination on amino acids metabolism in Spinacia oleracea L
    Milan Pavlík
    Isotope Laboratory, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic
    Ecotoxicol Environ Saf 73:1309-13. 2010
    ..05). The changes of its concentrations could be affected by changes of pH in plant cell or induction of alanine aminotransferase by hypoxia...
  4. pmc N-formylkynurenine as a marker of high light stress in photosynthesis
    Tina M Dreaden
    School of Chemistry and Biochemistry and Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    J Biol Chem 286:22632-41. 2011
    ..Reactive oxygen species reactions lead to oxidative damage of the reaction center, D1 protein turnover, and inhibition of electron transfer. Our results are consistent with a role for the CP43 NFK modification in photoinhibition...
  5. ncbi Proteome map of the chloroplast lumen of Arabidopsis thaliana
    Maria Schubert
    Department of Medical Nutrition, Karolinska Institute, Novum, Huddinge SE 14186, Sweden
    J Biol Chem 277:8354-65. 2002
    ..As a complement to our experimental investigation, we made a theoretical prediction of the luminal proteins from the whole Arabidopsis genome and estimated that the thylakoid lumen of the chloroplast contains approximately 80 proteins...
  6. ncbi Crystal structure of the non-regulatory A(4 )isoform of spinach chloroplast glyceraldehyde-3-phosphate dehydrogenase complexed with NADP
    S Fermani
    Dipartimento di Chimica G Ciamician, Universita di Bologna, Via Selmi 2, Bologna, 40126, Italia
    J Mol Biol 314:527-42. 2001
    ..This bridge is not found in other cytosolic or chloroplast GAPDHs from animals, bacteria, or plants other than spinach...
  7. ncbi Involvement of active oxygen species in degradation of light-harvesting proteins under light stresses
    Lello Zolla
    Department of Environmental Science, University of Tuscia, Viterbo, Italy
    Biochemistry 41:14391-402. 2002
    ....
  8. ncbi Relationship between activity, D1 loss, and Mn binding in photoinhibition of photosystem II
    A Krieger
    Section de Bioénergétique CNRS URA 2096, CEA Saclay, Gif sur Yvette, France
    Biochemistry 37:16262-9. 1998
    ..It is proposed that Mn binding protects against D1 loss by maintaining a protein structure which is not accessible to cleavage...
  9. ncbi Quality control of photosystem II under light stress - turnover of aggregates of the D1 protein in vivo
    Satoshi Ohira
    Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Photosynth Res 84:29-33. 2005
    ..Consistent with this notion, singlet oxygen production in thylakoid samples under illumination was shown to be stimulated significantly at higher temperatures...
  10. ncbi Malondialdehyde generated from peroxidized linolenic acid causes protein modification in heat-stressed plants
    Yasuo Yamauchi
    Graduate School of Agricultural Science, Kobe University, Kobe, Japan
    Plant Physiol Biochem 46:786-93. 2008
    ..This modification was not observed in linolenic acid-deficient mutants (fad3fad7fad8 triple mutant), suggesting that linolenic acid is a major source of protein modification by MDA in heat-stressed plants...
  11. pmc Hydrogen sulphide enhances photosynthesis through promoting chloroplast biogenesis, photosynthetic enzyme expression, and thiol redox modification in Spinacia oleracea seedlings
    Juan Chen
    Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, PR China
    J Exp Bot 62:4481-93. 2011
    ..In this report, the role of H(2)S in modulating photosynthesis of Spinacia oleracea seedlings was investigated. The main results are as follows...
  12. ncbi Photoproduction of catalase-insensitive peroxides on the donor side of manganese-depleted photosystem II: evidence with a specific fluorescent probe
    Sergey A Khorobrykh
    Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia
    Biochemistry 50:10658-65. 2011
    ..These ROOH forms, likely including several species derived from lipid peroxides, may mediate the donor side-induced photoinhibition of PSII via protein modification...
  13. ncbi Characterization of interactions between Escherichia coli O157:H7 with epiphytic bacteria in vitro and on spinach leaf surfaces
    Gabriela Lopez-Velasco
    Department of Food Science and Technology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Int J Food Microbiol 153:351-7. 2012
    ....
  14. ncbi Multistate outbreak of Escherichia coli O157:H7 infection associated with consumption of packaged spinach, August-September 2006: the Wisconsin investigation
    Arthur M Wendel
    Wisconsin Division of Public Health, Madison, Wisconsin, USA
    Clin Infect Dis 48:1079-86. 2009
    ..Escherichia coli O157:H7 infection often causes hemorrhagic colitis and hemolytic uremic syndrome...
  15. pmc Quality control of photosystem II: reactive oxygen species are responsible for the damage to photosystem II under moderate heat stress
    Amu Yamashita
    Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    J Biol Chem 283:28380-91. 2008
    ..Although occurring in the dark, the damages caused by the moderate heat stress to photosystem II are quite similar to those induced by excessive illumination where reactive oxygen species are involved...
  16. ncbi Formation of radicals from singlet oxygen produced during photoinhibition of isolated light-harvesting proteins of photosystem II
    Sara Rinalducci
    Department of Environmental Sciences, University of Tuscia, Largo dell Universita, 01100 Viterbo, Italy
    Biochim Biophys Acta 1608:63-73. 2004
    ..Thus, monomerization of antennae as well as their incorporation into the PSII complex seem to represent physiologically protected forms. A comparison is made of the photoinhibition mechanisms of different photosynthetic systems...
  17. ncbi Specific plasma membrane aquaporins of the PIP1 subfamily are expressed in sieve elements and guard cells
    Laure C Fraysse
    Department of Plant Biochemistry, Lund University, P O Box 124, S 22100 Lund, Sweden
    Biol Cell 97:519-34. 2005
    ..As the nomenclature implies, the majority of PIPs have been shown to be localized at the plasma membrane. Recently, two highly abundant plasma membrane aquaporins, SoPIP2;1 and SoPIP1;2, have been purified and structurally characterized...
  18. ncbi Quenching of excited states of chlorophyll molecules in submembrane fractions of Photosystem I by exogenous quinones
    Subramanyam Rajagopal
    Groupe de Recherche en Energie et Information Biomoléculaires, Universite du Quebec a Trois Rivieres, 3351 Boulevard des Forges, Three Rivers, Quebec, Canada GA9 5H7
    Biochim Biophys Acta 1606:147-52. 2003
    ....
  19. ncbi Quantitative analysis of the experimental O-J-I-P chlorophyll fluorescence induction kinetics. Apparent activation energy and origin of each kinetic step
    Steve Boisvert
    Groupe de Recherche en Biologie Végétale, GRBV, Universite du Quebec a Trois Rivieres, Quebec, Canada
    FEBS J 273:4770-7. 2006
    ....
  20. pmc Extended-spectrum Beta-lactamase gene sequences in gram-negative saprophytes on retail organic and nonorganic spinach
    Eva Raphael
    Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, 201 Hildebrand Hall, Berkeley, CA 94720 7360, USA
    Appl Environ Microbiol 77:1601-7. 2011
    ....
  21. ncbi Modulation of flash-induced photosystem II fluorescence by events occurring at the water oxidizing complex
    I I Putrenko
    Department of Biological Sciences, Brock University, St Catharines, Ontario, Canada
    Biochemistry 38:10632-41. 1999
    ....
  22. ncbi Coenzyme site-directed mutants of photosynthetic A4-GAPDH show selectively reduced NADPH-dependent catalysis, similar to regulatory AB-GAPDH inhibited by oxidized thioredoxin
    Francesca Sparla
    Laboratorio di Fisiologia molecolare delle piante, Dipartimento di Biologia Evoluzionistica Sperimentale, Via Irnerio 42, Universita di Bologna, I 40126 Bologna, Italy
    J Mol Biol 340:1025-37. 2004
    ..A model based on this concept might be developed to explain the in vivo light-regulation of the GAPDH...
  23. ncbi Development of a dynamic growth-death model for Escherichia coli O157:H7 in minimally processed leafy green vegetables
    Robin C McKellar
    AAFC Research Associate, Central Experimental Farm, 960 Carling Ave, Ottawa, Ontario, Canada K1N0C6
    Int J Food Microbiol 151:7-14. 2011
    ..By incorporating variability, the resulting model is an improvement over existing ones, and is suitable for QRA applications...
  24. ncbi Behavior of Escherichia coli O157:H7 on damaged leaves of spinach, lettuce, cilantro, and parsley stored at abusive temperatures
    Rowaida K Khalil
    Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt
    J Food Prot 73:212-20. 2010
    ..This study provides evidence that the damaged portion of a leafy green is a distinct growth niche that elicits different microbial responses in the various types of leafy greens...
  25. ncbi The structure of the chloroplast F1-ATPase at 3.2 A resolution
    G Groth
    Heinrich Heine Universitat, Biochemie der Pflanzen, Universitatsstrasse 1, D 40225 Dusseldorf, Germany and EMBL Hamburg, Notkestrasse 85, D 22603 Hamburg, Germany
    J Biol Chem 276:1345-52. 2001
    ..The structure probably represents an inactive latent state of the ATPase, which is unique to chloroplast and cyanobacterial enzymes...
  26. ncbi Identification of a mechanism of photoprotective energy dissipation in higher plants
    Alexander V Ruban
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK
    Nature 450:575-8. 2007
    ..We suggest that this is the principal mechanism of photoprotection...
  27. ncbi Assay of photoinhibition of photosystem II and protease activity
    Yasusi Yamamoto
    Graduate School of Natural Science and Technology, Okayama University, Japan
    Methods Mol Biol 274:217-27. 2004
    ..The method to analyze the protease activity in the stroma that recognizes and digests the crosslinked products of the D1 protein generated by the light stress is also described...
  28. ncbi Purification and characterization of Delta(1)-pyrroline-5-carboxylate reductase isoenzymes, indicating differential distribution in spinach (Spinacia oleracea L.) leaves
    M Murahama
    Department of Biology, Faculty of Science, Kanazawa University, Kakuma, Kanazawa, 920 1192, Japan
    Plant Cell Physiol 42:742-50. 2001
    ..NAD(P)-5-oxidoreductase), the second enzyme in the proline biosynthetic pathway, was purified from spinach (Spinacia oleracea L.) leaves...
  29. ncbi Singlet oxygen scavenging activity of plastoquinol in photosystem II of higher plants: electron paramagnetic resonance spin-trapping study
    Deepak Kumar Yadav
    Laboratory of Biophysics, Department of Experimental Physics, Faculty of Science, Palacky University, Tr 17 listopadu 12, Olomouc, Czech Republic
    Biochim Biophys Acta 1797:1807-11. 2010
    ..These observations reveal that plastoquinol exogenously added to PQ-depleted PSII membranes serves as efficient scavenger of ¹O₂...
  30. ncbi Iodine uptake by spinach (Spinacia oleracea L.) plants grown in solution culture: effects of iodine species and solution concentrations
    Y G Zhu
    Department of Soil Environmental Sciences, Research Centre for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
    Environ Int 29:33-7. 2003
    ..out to investigate the effects of iodine species and solution concentrations on iodine uptake by spinach (Spinacia oleracea L.)...
  31. ncbi The 1.49 A resolution crystal structure of PsbQ from photosystem II of Spinacia oleracea reveals a PPII structure in the N-terminal region
    Monica Balsera
    Instituto de Recursos Naturales y Agrobiología CSIC, Cordel de Merinas 52, 37008 Salamanca, Spain
    J Mol Biol 350:1051-60. 2005
    ..In addition, the PsbQ crystal coordinates two zinc ions, one of them is proposed to have a physiological role in higher plants, on the basis of the full conservation of the ligand protein residues in the sequence subfamily...
  32. pmc Amino acid residues critical for the specificity for betaine aldehyde of the plant ALDH10 isoenzyme involved in the synthesis of glycine betaine
    Angel G Díaz-Sánchez
    Departamento de Bioquimica, Facultad de Quimica, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, 04510 Mexico Distrito Federal, Mexico
    Plant Physiol 158:1570-82. 2012
    ..of ω-primary or ω-quaternary aminoaldehydes, but, intriguingly, only some of them, such as the spinach (Spinacia oleracea) betaine aldehyde dehydrogenase (SoBADH), efficiently oxidize betaine aldehyde (BAL) forming the ..
  33. pmc Identification of oxidized amino acid residues in the vicinity of the Mn(4)CaO(5) cluster of Photosystem II: implications for the identification of oxygen channels within the Photosystem
    Laurie K Frankel
    Department of Biological Sciences, Division of Biochemistry and Molecular Biology, Louisiana State University, Baton Rouge, LA 70803, USA
    Biochemistry 51:6371-7. 2012
    ..These results are discussed within the context of a number of computational studies which have identified putative oxygen channels within the photosystem...
  34. ncbi Photoconsumption of oxygen in photosystem II preparations under impairment of the water-oxidizing complex
    S A Khorobrykh
    Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia
    Biochemistry (Mosc) 67:683-8. 2002
    ....
  35. ncbi Three-dimensional electron cryo-microscopy study of the extrinsic domains of the oxygen-evolving complex of spinach: assignment of the PsbO protein
    Jon Nield
    Wolfson Laboratories, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AY, United Kingdom
    J Biol Chem 277:15006-12. 2002
    ..This structural assignment is discussed in terms of the recent x-ray-derived cyanobacterial model of PSII (Zouni, A., Witt, H.-T., Kern, J., Fromme, P., Krauss, N., Saenger, W., and Orth, P. (2001) Nature 409, 739-743)...
  36. ncbi Dynamic Interaction between the D1 protein, CP43 and OEC33 at the lumenal side of photosystem II in spinach chloroplasts: evidence from light-induced cross-Linking of the proteins in the donor-side photoinhibition
    Takahiro Henmi
    Graduate School of Natural Science and Technology, Okayama University, Okayama, 700 8530 Japan
    Plant Cell Physiol 44:451-6. 2003
    ..Acad. Sci. USA, 100: 98]. Moreover, the data suggest that the binding condition and positioning of the OEC33 in the photosystem II complex from higher plants may be different from those in cyanobacteria...
  37. ncbi Enhanced susceptibility of photosynthesis to low-temperature photoinhibition due to interruption of chill-induced increase of S-adenosylmethionine decarboxylase activity in leaves of spinach (Spinacia oleracea L.)
    Lixiong He
    Department of Life Science, Faculty of Bioresources, Mie University, Tsu, 514 8507 Japan
    Plant Cell Physiol 43:196-206. 2002
    The possible involvement of polyamines in the chilling tolerance of spinach (Spinacia oleracea L.) was investigated focusing on photosynthesis...
  38. pmc Evidence that cytochrome b559 is involved in superoxide production in photosystem II: effect of synthetic short-chain plastoquinones in a cytochrome b559 tobacco mutant
    Pavel Pospisil
    Laboratory of Biophysics, Department of Experimental Physics, Faculty of Science, Palacky University, tr Svobody 26, 771 46 Olomouc, Czech Republic
    Biochem J 397:321-7. 2006
    ..Based on these findings it is suggested that short-chain PQs stimulate O2*- production via a mechanism that involves electron transfer from Pheo- (pheophytin) to LP cyt b559 and subsequent auto-oxidation of LP cyt b559...
  39. ncbi Structural mechanism of plant aquaporin gating
    Susanna Törnroth-Horsefield
    Department of Chemistry and Bioscience, Chalmers University of Technology, P O Box 462, SE 40530 Goteborg, Sweden
    Nature 439:688-94. 2006
    ..These results reveal a molecular gating mechanism which appears conserved throughout all plant plasma membrane aquaporins...
  40. ncbi Structure and function of the PsbP protein of photosystem II from higher plants
    Kentaro Ifuku
    Graduate School of Biostudies, Kyoto University, Kyoto 606 8502, Japan
    Photosynth Res 84:251-5. 2005
    ..The possible N-terminal residues of PsbP that would be involved in this interaction are discussed...
  41. ncbi Quality control of Photosystem II: cleavage and aggregation of heat-damaged D1 protein in spinach thylakoids
    Keisuke Komayama
    Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Biochim Biophys Acta 1767:838-46. 2007
    ..The cleavage and aggregation of the D1 protein appear to be two alternative processes influenced by protein phosphorylation/dephosphorylation, distribution of FtsH, and intactness of the thylakoids...
  42. ncbi Mapping of the genes for dioecism and monoecism in Spinacia oleracea L.: evidence that both genes are closely linked
    Yasuyuki Onodera
    Laboratory of Genetic Engineering, Research Faculty of Agriculture, Hokkaido University, N 9, W 9, Sapporo 060 8589, Japan
    Plant Cell Rep 30:965-71. 2011
    ..SO4 will enable breeders to efficiently select highly male monoecious plants for preferential use as the pollen parent for hybrid seed production...
  43. ncbi Effect of organic and conventional cropping systems on ascorbic acid, vitamin C, flavonoids, nitrate, and oxalate in 27 varieties of spinach (Spinacia oleracea L.)
    Eunmi Koh
    Department of Food Science and Technology, University of California at Davis, One Shields Avenue, Davis, California 95616, USA
    J Agric Food Chem 60:3144-50. 2012
    ..These results suggest that organic cropping systems result in spinach with lower levels of nitrates and higher levels of flavonoids and ascorbic acid...
  44. ncbi Documentation of significant electron transport defects on the reducing side of photosystem II upon removal of the PsbP and PsbQ extrinsic proteins
    Johnna L Roose
    Department of Biological Sciences, Biochemistry and Molecular Biology Section, Louisiana State University, Baton Rouge, Louisiana 70803, USA
    Biochemistry 49:36-41. 2010
    ..6 M urea did not induce further reducing-side alterations. Our results demonstrate that studies attributing defects observed upon PsbP and PsbQ removal solely to the oxidizing side must be viewed with caution...
  45. pmc Spinacia oleracea L. leaf stomata harboring Cryptosporidium parvum oocysts: a potential threat to food safety
    Dumitru Macarisin
    United States Department of Agriculture Agricultural Research Service USDA ARS, Beltsville Agricultural Research Center BARC, Environmental Microbial and Food Safety Laboratory, 10300 Baltimore Avenue, Bldg 173, BARC East, Beltsville, MD 20705, USA
    Appl Environ Microbiol 76:555-9. 2010
    ..These findings and the fact that this pathogenic parasite resists washing and disinfection raise concerns regarding food safety...
  46. ncbi Valinomycin sensitivity proves that light-induced thylakoid voltages result in millisecond phase of chlorophyll fluorescence transients
    Pavel Pospisil
    FB Physik, Freie Universitat Berlin, Arminallee 14, D 14195 Dahlem, Berlin, Germany
    Biochim Biophys Acta 1554:94-100. 2002
    ..A putative role of thylakoid voltages in protection of PSII against photoinhibitory damage is discussed...
  47. ncbi Chlorophyll thermoluminescence of leaf discs: simple instruments and progress in signal interpretation open the way to new ecophysiological indicators
    Jean Marc Ducruet
    Service de Bioenergetique, Bat 532, INRA CEA Saclay, 91191 Gif sur Yvette Cedex, France
    J Exp Bot 54:2419-30. 2003
    ....
  48. pmc Structure of the c14 rotor ring of the proton translocating chloroplast ATP synthase
    Melanie Vollmar
    Institut für Biochemie der Pflanzen, Heinrich Heine Universitat Dusseldorf, 40225 Dusseldorf, Germany
    J Biol Chem 284:18228-35. 2009
    ..Reprotonation of Glu(61) by a conserved arginine in the adjacent a subunit returns the carboxylate to its initial conformation...
  49. pmc Solution structures of spinach acyl carrier protein with decanoate and stearate
    Gregory A Zornetzer
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706 1549, USA
    Biochemistry 45:5217-27. 2006
    ..These structures demonstrate the conformational flexibility of spinach ACP and suggest how the protein changes to accommodate its myriad binding partners...
  50. ncbi The 5A structure of heterologously expressed plant aquaporin SoPIP2;1
    W Kukulski
    Maurice E Müller Institute for Microscopy, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    J Mol Biol 350:611-6. 2005
    ..This crystal packing arrangement provides access to the phosphorylated C terminus as well as the loop B phosphorylation site for studies of channel gating...
  51. ncbi Flippase activity in proteoliposomes reconstituted with Spinacea oleracea endoplasmic reticulum membrane proteins: evidence of biogenic membrane flippase in plants
    Santosh Kumar Sahu
    Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India
    Biochemistry 47:10481-90. 2008
    ..These results suggest the existence of biogenic membrane flippases in plants and that the mechanism of membrane biogenesis is similar to that found in animals...
  52. ncbi Structural and functional self-organization of Photosystem II in grana thylakoids
    Helmut Kirchhoff
    Institut fur Botanik, Schlossgarten 3, D 48149 Munster, Germany
    Biochim Biophys Acta 1767:1180-8. 2007
    ..This indicates that the supramolecular protein arrangement in grana thylakoids is a self-organized process...
  53. ncbi Spinacia oleracea L. protects against gamma radiations: a study on glutathione and lipid peroxidation in mouse liver
    A L Bhatia
    Department of Zoology, Radiation Biology Laboratory, University of Rajasthan, Jaipur, India
    Phytomedicine 11:607-15. 2004
    The present study deals with the protective effect of Spinacia oleracea L. against radiation-induced oxidative stress, which is evaluated in terms of lipid peroxidation (LPO) product and tissue levels of glutathione...
  54. ncbi Interaction of Escherichia coli with growing salad spinach plants
    Keith Warriner
    Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK
    J Food Prot 66:1790-7. 2003
    ..coli. The implications of this study's findings with regard to the microbiological safety of minimally processed vegetables are discussed...
  55. ncbi The nuclear RPL4 gene encodes a chloroplast protein that co-purifies with the T7-like transcription complex as well as plastid ribosomes
    Y Trifa
    Laboratoire de Génétique Moléculaire des Plantes, Université Joseph Fourier and Centre National de la Recherche Scientifique, B P 53, F 38041 Grenoble, France
    J Biol Chem 273:3980-5. 1998
    ....
  56. ncbi Lipids in oxygen-evolving photosystem II complexes of cyanobacteria and higher plants
    Isamu Sakurai
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902
    J Biochem 140:201-9. 2006
    ..These findings suggest that phosphatidylglycerol plays important roles in the electron transport at the QB-binding site in photosystem II complexes...
  57. ncbi Trypsin inhibitors from the garden four o'clock (Mirabilis jalapa) and spinach (Spinacia oleracea) seeds: isolation, characterization and chemical synthesis
    Jolanta Kowalska
    Faculty of Biotechnology, University of Wrocław, Tamka 2, 50 137 Wrocław, Poland
    Phytochemistry 68:1487-96. 2007
    Five serine proteinase inhibitors (Mirabilis jalapa trypsin inhibitors, MJTI I and II and Spinacia oleracea trypsin inhibitors, SOTI I, II, and III) from the garden four-o'clock (M. jalapa) and spinach (S. oleracea) seeds were isolated...
  58. ncbi Differences in the metabolite profiles of spinach (Spinacia oleracea L.) leaf in different concentrations of nitrate in the culture solution
    Keiki Okazaki
    National Agricultural Research Center for Hokkaido Region, Sapporo 062 8555, Japan
    Plant Cell Physiol 49:170-7. 2008
    ....
  59. ncbi Probing the organization of photosystem II in photosynthetic membranes by atomic force microscopy
    Helmut Kirchhoff
    Institut fur Botanik, Schlossgarten 3, D 48149 Munster, Germany
    Biochemistry 47:431-40. 2008
    ..The functional consequences for lateral migration processes are discussed...
  60. ncbi Arrangement of photosystem II supercomplexes in crystalline macrodomains within the thylakoid membrane of green plant chloroplasts
    E J Boekema
    Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, 9747 AG, The Netherlands
    J Mol Biol 301:1123-33. 2000
    ..The implications of this type of organization for the efficient transfer of excitation energy from LHCII to PSII and for the stacking of grana membranes are discussed...
  61. ncbi Sequence evolution and sex-specific expression patterns of the C class floral identity gene, SpAGAMOUS, in dioecious Spinacia oleracea L
    D Noah Sather
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    Planta 222:284-92. 2005
    Development in dioecious cultivated spinach, Spinacia oleracea, is distinguished by the absence of alternative reproductive organ primordia in male and female flowers...
  62. ncbi Re-consideration of Peronospora farinosa infecting Spinacia oleracea as distinct species, Peronospora effusa
    Young Joon Choi
    Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul 136 701, Korea
    Mycol Res 111:381-91. 2007
    Downy mildew is probably the most widespread and potentially destructive global disease of spinach (Spinacia oleracea)...
  63. ncbi CPD photolyase gene from Spinacia oleracea: repair of UV-damaged DNA and expression in plant organs
    Ryouhei Yoshihara
    The United Graduate School of Agricultural Sciences, Tottori University, Japan
    J Radiat Res 46:157-64. 2005
    ..In both the male and female plants, the gene was highly expressed in leaves and flowers under the condition of 14-h light and 10-h dark cycle. The expression in the roots was quite low compared with the other organs...
  64. ncbi Characterization of SpAPETALA3 and SpPISTILLATA, B class floral identity genes in Spinacia oleracea, and their relationship to sexual dimorphism
    Catherine Pfent
    School of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA
    Dev Genes Evol 215:132-42. 2005
    ..These observations suggest that regulation of B class floral identity genes has a role in the development of sexual dimorphism and dioecy in spinach rather than being a secondary result of organ abortion...
  65. ncbi Localization of the 23-kDa subunit of the oxygen-evolving complex of photosystem II by electron microscopy
    E J Boekema
    Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Eur J Biochem 252:268-76. 1998
    ..5 nm. Measured from the centres of the masses, the two 33-kDa subunits associated with the dimeric PSII-LHCII SC are separated by 6.3 nm. The corresponding distance between the two 23-kDa subunits is 8.8 nm...
  66. ncbi Reconstitution of water channel function of an aquaporin overexpressed and purified from Pichia pastoris
    Maria Karlsson
    Department of Plant Biochemistry, Lund University, Box 124, Sweden
    FEBS Lett 537:68-72. 2003
    ..PM28A was efficiently solubilized from urea- and alkali-stripped Pichia membranes by octyl-beta-D-thioglucopyranoside resulting in a final yield of 25 mg of purified protein per liter of cell culture...
  67. ncbi CNS depressive role of aqueous extract of Spinacia oleracea L. leaves in adult male albino rats
    Sutapa Das
    S N Pradhan Centre for Neursciences, University of Calcutta, Kolkata, India
    Indian J Exp Biol 46:185-90. 2008
    Treatment with Spinacia oleracea extract (SO; 400 mg/kg body weight) decreased the locomotor activity, grip strength, increased pentobarbitone induced sleeping time and also markedly altered pentylenetetrazole induced seizure status in ..
  68. ncbi The plastid chromosome of spinach (Spinacia oleracea): complete nucleotide sequence and gene organization
    C Schmitz-Linneweber
    , , Germany
    Plant Mol Biol 45:307-15. 2001
    The chloroplast chromosome of spinach (Spinacia oleracea) is a double-stranded circular DNA molecule of 150,725 nucleotide pairs...
  69. pmc Arrangement of photosystem II and ATP synthase in chloroplast membranes of spinach and pea
    Bertram Daum
    Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany
    Plant Cell 22:1299-312. 2010
    ..Grana formation is likely to result from electrostatic interactions between these complexes across the stromal gap...
  70. ncbi Interactions between Spinacia oleracea and Bradysia impatiens: a role for phytoecdysteroids
    Eric A Schmelz
    Department of Entomology, University of Arizona, Tucson, USA
    Arch Insect Biochem Physiol 51:204-21. 2002
    ..In spinach (Spinacia oleracea), 20-hydroxyecdysone (20E) concentrations in the roots rapidly increase following root damage, root ..
  71. ncbi Low-light-induced formation of semicrystalline photosystem II arrays in higher plant chloroplasts
    Helmut Kirchhoff
    Institute of Botany, Schlossgarten 3, D 48149 Munster, Germany
    Biochemistry 46:11169-76. 2007
    ..Furthermore, the occurrence of a hexagonal phase of the lipid monogalactosyldiacylglycerol in grana membranes of low-light-adapted plants could trigger the rearrangement by changing the lateral membrane pressure...
  72. ncbi Recirculating immunomagnetic separation and optimal enrichment conditions for enhanced detection and recovery of low levels of Escherichia coli O157:H7 from fresh leafy produce and surface water
    Sunee Himathongkham
    Food and Drug Laboratory Branch, California Department of Public Health, Richmond, California 94804, USA
    J Food Prot 70:2717-24. 2007
    ..The method used in this study was effective for monitoring E. coli O157:H7 in the farm environment, during postharvest processing, and in foodborne outbreak investigations...
  73. ncbi Involvement of uncoupled antenna chlorophylls in photoinhibition in thylakoids
    S Santabarbara
    Centro C.N.R. Biologia Cellulare e Molecolare delle Piante, Dipartamento de Biologia, , Via Celoria 26, 20133 Milan, Italy
    FEBS Lett 491:109-13. 2001
    ..The broadness of the action spectrum suggests that degraded or incompletely assembled pigment-protein complexes may be involved...
  74. pmc Pathways and timescales of primary charge separation in the photosystem II reaction center as revealed by a simultaneous fit of time-resolved fluorescence and transient absorption
    Vladimir I Novoderezhkin
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow, Russia
    Biophys J 89:1464-81. 2005
    ....
  75. ncbi Thioredoxin target proteins in chloroplast thylakoid membranes
    Yves Balmer
    Department of Plant and Microbial Biology, University of California, Berkeley, 94720, USA
    Antioxid Redox Signal 8:1829-34. 2006
    ....
  76. ncbi Carotenoid specificity of light-harvesting complex II binding sites. Occurrence of 9-cis-violaxanthin in the neoxanthin-binding site in the parasitic angiosperm Cuscuta reflexa
    Alison M Snyder
    School of Biological Sciences, University of Canterbury, Christchurch 8020, New Zealand
    J Biol Chem 279:5162-8. 2004
    ..These results suggest that the N1 binding site of LHCIIb preferentially binds 9-cis-5,6-epoxy carotenoids, which has implications for the features of this binding site and its role in the photosystem II antenna assembly and stability...
  77. ncbi Effects of acid and alkali on the light absorption, energy transfer and protein secondary structures of core antenna subunits CP43 and CP47 of photosystem II
    Shukui Guo
    Key Lab of Photosynthesis and Environment Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, People s Republic of China
    Photochem Photobiol 79:291-6. 2004
    ....
  78. ncbi Perturbation of the structure of P680 and the charge distribution on its radical cation in isolated reaction center complexes of photosystem II as revealed by fourier transform infrared spectroscopy
    Tatsunori Okubo
    Institute of Materials Science, University of Tsukuba, Tsukuba, Ibaraki 305 8573, Japan
    Biochemistry 46:4390-7. 2007
    ..This potential decrease explains the well-known phenomenon that YZ is not oxidized by P680+ in RC complexes...
  79. ncbi Effects of nano-anatase on spectral characteristics and distribution of LHCII on the thylakoid membranes of spinach
    Lei Zheng
    College of Life Sciences, Suzhou University, Suzhou 215123, People s Republic of China
    Biol Trace Elem Res 120:273-83. 2007
    ....
  80. ncbi The stoichiometry of the two photosystems in higher plants revisited
    Da Yong Fan
    Photobioenergetics Group, Research School of Biological Sciences, The Australian National University, Canberra, ACT 0200, Australia
    Biochim Biophys Acta 1767:1064-72. 2007
    ....
  81. ncbi Multidimensional proteomic analysis of photosynthetic membrane proteins by liquid extraction-ultracentrifugation-liquid chromatography-mass spectrometry
    Christian G Huber
    Department of Chemistry, Instrumental Analysis and Bioanalysis, Saarland University, Saarbrucken, Germany
    Proteomics 4:3909-20. 2004
    ..Because of its high flexibility and suitability for proteins having a very wide range of Mr and hydrophobicities, the method is generally applicable to the analysis of complex mixtures of membrane proteins...
  82. ncbi Light absorption by the chlorophyll a-b complexes of photosystem II in a leaf with special reference to LHCII
    Andrea Rivadossi
    Istituto di Biofisica del Consiglio Nazionale delle Ricerche Sezione di Milano, Dipartimento di Biologia, Universita degli Studi di Milano, Milano, Italy
    Photochem Photobiol 80:492-8. 2004
    ..The relative enhancement of the chl b absorption is principally associated with the Qy electronic transition region, the light-harvesting contribution of which becomes prominent in the leaf...
  83. ncbi Investigation of the lateral light-induced migration of photosystem II light-harvesting proteins by nano-high performance liquid chromatography electrospray ionization mass spectrometry
    Anna M Timperio
    Department of Environmental Science, University of Tuscia, 01100 Viterbo, Italy
    J Biol Chem 280:28858-66. 2005
    ..We hypothesize that the Lhcb1 isoforms expressed by the multigene families play a role in plant adaptation...
  84. pmc Diffusion of light-harvesting complex II in the thylakoid membranes
    Elena Consoli
    Department of Biology and Interdisciplinary Center for Nanostructured Materials and Interfaces CIMAINA, University of Milan, Italy
    EMBO Rep 6:782-6. 2005
    ..We found that the population of P-LHCII in unappressed membranes is more mobile than the population of non-P-LHCII from the same regions...
  85. ncbi Carotenoid cation formation and the regulation of photosynthetic light harvesting
    Nancy E Holt
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Science 307:433-6. 2005
    ..These results indicate that energy transfer from chlorophyll molecules to a chlorophyllzeaxanthin heterodimer, which then undergoes charge separation, is the mechanism for excess energy dissipation during feedback deexcitation...
  86. ncbi The glutathione peroxidase homologous gene Gpxh in Chlamydomonas reinhardtii is upregulated by singlet oxygen produced in photosystem II
    Beat B Fischer
    Department of Environmental Toxicology, Swiss Federal Institute for Environmental Science and Technology EAWAG, Uberlandstr 133, Dubendorf 8600, Switzerland
    Planta 223:583-90. 2006
    ..This indicates that the Gpxh expression is induced by 1O2 under environment conditions causing photoinhibition...
  87. ncbi The mobile thylakoid phosphoprotein TSP9 interacts with the light-harvesting complex II and the peripheries of both photosystems
    Maria Hansson
    Division of Cell Biology, Linkoping University, SE 581 85 Linkoping, Sweden
    J Biol Chem 282:16214-22. 2007
    ..An association of TSP9 with LHCII as well as the peripheries of the photosystems suggests its involvement in regulation of photosynthetic light harvesting...
  88. ncbi Rapid loss of structural motifs in the manganese complex of oxygenic photosynthesis by X-ray irradiation at 10-300 K
    Markus Grabolle
    Freie Universitat Berlin, FB Physik, Arnimallee 14, D 14195 Berlin, Germany
    J Biol Chem 281:4580-8. 2006
    ..It is proposed to employ controlled x-ray photoreduction in metalloprotein research for: (i) population of distinct reduced states, (ii) estimating the redox potential of buried metal centers, and (iii) research on protein dynamics...
  89. ncbi Dimeric and monomeric organization of photosystem II. Distribution of five distinct complexes in the different domains of the thylakoid membrane
    Ravi Danielsson
    Department of Biochemistry, Center for Chemistry and Chemical Engineering, P O Box 124, Lund University, S 221 00 Lund, Sweden
    J Biol Chem 281:14241-9. 2006
    ..PSII core monomers were heterogeneous, and a large fraction did not evolve oxygen. PSII monomers without the CP43 protein and PSII reaction centers showed no oxygen-evolving activity...
  90. ncbi Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macro-assemblies with light-inducible structural transitions
    Ilian Simidjiev
    Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Szeged
    Methods Mol Biol 274:105-14. 2004
    ....
  91. ncbi Biochemical properties of the PsbS subunit of photosystem II either purified from chloroplast or recombinant
    Paola Dominici
    Dipartimento Scientifico e Tecnologico, Universita degli Studi di Verona, 37134 Verona, Italy
    J Biol Chem 277:22750-8. 2002
    ..This domain is suggested to be involved in sensing low luminal pH...
  92. pmc In vitro reconstitution of the activated zeaxanthin state associated with energy dissipation in plants
    Mark Aspinall-O'Dea
    Robert Hill Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    Proc Natl Acad Sci U S A 99:16331-5. 2002
    ..The altered properties of zeaxanthin and PsbS that result from this interaction provide the first direct indication about how they regulate energy dissipation...
  93. pmc Polyphenolic allelochemicals from the aquatic angiosperm Myriophyllum spicatum inhibit photosystem II
    Eva Leu
    Limnologiesches Institut, Universitat Konstanz, Freiburg, Germany
    Plant Physiol 130:2011-8. 2002
    ..Separate measurements of photosystem I and II activity with spinach (Spinacia oleracea) thylakoid membranes indicated that the site of inhibition is located at photosystem II (PSII)...
  94. ncbi Singlet oxygen production in herbicide-treated photosystem II
    Christian Fufezan
    Institut fur Biologie II, Biochemie der Pflanzen, Universitat Freiburg, Schänzlestr 1, 79104, Freiburg, Germany
    FEBS Lett 532:407-10. 2002
    ..When phenolic herbicides are bound, charge recombination at the level of P+*Pheo-* is thermodynamically favoured forming a chlorophyll triplet and hence 1O2. With urea herbicides this pathway is less favourable...
  95. ncbi Light-induced trimer to monomer transition in the main light-harvesting antenna complex of plants: thermo-optic mechanism
    Gyozo Garab
    Institutes of Plant Biology and Biophysics, Biological Research Center, Hungarian Academy of Sciences, P O Box 521, H 6701 Szeged, Hungary
    Biochemistry 41:15121-9. 2002
    ..Structural changes driven by thermo-optic mechanisms may therefore provide plants with a novel mechanism for regulation of light harvesting in excess light...
  96. ncbi Energy dissipation in photosynthesis: does the quenching of chlorophyll fluorescence originate from antenna complexes of photosystem II or from the reaction center?
    N G Bukhov
    Julius von Sachs Institut fur Biowissenschaften, Universitat Wurzburg, 97082 Wurzburg, Germany
    Planta 212:749-58. 2001
    ..Warnst., and in leaves of Spinacia oleracea L. and Arabidopsis thaliana (L.) Heynh., using chlorophyll fluorescence as an indicator reaction...
  97. ncbi A few molecules of zeaxanthin per reaction centre of photosystem II permit effective thermal dissipation of light energy in photosystem II of a poikilohydric moss
    N G Bukhov
    , , , Germany
    Planta 212:739-48. 2001
    ..20% CO2 in air was incapable of quenching basal, so-called F0 fluorescence either in the moss or in spinach (Spinacia oleracea L.) leaves...
  98. pmc Energy transfer in light-harvesting complexes LHCII and CP29 of spinach studied with three pulse echo peak shift and transient grating
    Jante M Salverda
    Department of Biophysics and Physics of Complex Systems, Division of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit Amsterdam, The Netherlands
    Biophys J 84:450-65. 2003
    ..Our study is the first to provide spectroscopic evidence for mixed binding sites, as well as the first to show their existence in native complexes...
  99. ncbi Oxidation of the two beta-carotene molecules in the photosystem II reaction center
    Alison Telfer
    Wolfson Laboratories, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK
    Biochemistry 42:1008-15. 2003
    ..Although contributions of chlorophyll cations cannot be formally ruled out, our results demonstrate that these spectra mainly arise from the cation radical species of the two carotenoids present in photosystem II reaction centers...
  100. pmc Active oxygen produced during selective excitation of photosystem I is damaging not only to photosystem I, but also to photosystem II
    S E Tjus
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 125:2007-15. 2001
    ..aim to specifically study the molecular mechanisms behind photoinhibition of photosystem I, stacked spinach (Spinacia oleracea) thylakoids were irradiated at 4 degrees C with far-red light (>715 nm) exciting photosystem I, but not ..
  101. pmc Posttranslational modifications in the CP43 subunit of photosystem II
    Lorraine B Anderson
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, St Paul 55108, USA
    Proc Natl Acad Sci U S A 99:14676-81. 2002
    ..As a step toward understanding the tertiary structure of this CP43 peptide, structural modeling was performed by using molecular dynamics...

Research Grants19

  1. Retinol Equivalence of Plant Carotenoids in Children
    Guangwen Tang; Fiscal Year: 2006
    ..This study will be of importance in planning vitamin A deficiency prevention strategies and also will provide useful information regarding the potential efficacy of a bioengineered crop to provide vitamin A nutrition. ..
  2. Thermodynamic Properties of NADH:Nitrate Reductase
    Michael Barber; Fiscal Year: 2005
    ..These studies will enhance our understanding of important structure-function relationships in NR and other related metalloflavoproteins. ..
  3. SPECTROSCOPIC STUDIES OF LIGHT-DRIVEN ELECTRON TRANSFER
    BRIDGETTE ANNE BARRY; Fiscal Year: 2010
    ..This specific aim will serve to elucidate the role of primary, secondary, and tertiary interactions in a structurally defined, tractable model system. ..
  4. Therapeutic vaccine against tuberculosis
    Lee Riley; Fiscal Year: 2006
    ..Therapeutic vaccines against TB is a major priority in TB control, and we have an opportunity to identify such products by a novel approach using a simple, inexpensive, and reproducible animal model. ..
  5. ANTIOXIDANT GENES OF MYCOBACERIUM TUBERCULOSIS
    Lee Riley; Fiscal Year: 2001
    ....
  6. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2010
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  7. MOLECULAR BIOLOGY OF INFECTION WITH M TUBERCULOSIS
    Lee Riley; Fiscal Year: 2002
    ..In addition, these studies may identify several potential targets for new chemotherapeutic agents or vaccine candidates. ..
  8. STRUCTURE AND FUNCTION OF CAROTENOIDS
    Harry Frank; Fiscal Year: 2008
    ..Several preliminary studies have already been carried out by us in support of the methods we propose to employ. ..
  9. In vivo regulation of M. tuberculosis cell wall lipids
    Lee W Riley; Fiscal Year: 2010
    ..tuberculosis and granulomas may contribute to our understanding of the mechanism of persistence of M. tuberculosis that could lead to new tests to differentiate active TB from latent TB infection. ..
  10. Orgin of multidrug resistant uropathogenic E. coli
    Lee Riley; Fiscal Year: 2008
    ..This knowledge should lead to the implementation of improved control of drug-resistant infectious diseases. ..
  11. ALCOHOL EFFECT ON HUMAN LACTATION AND INFANT BEHAVIOR
    JULIE MENNELLA; Fiscal Year: 2007
    ..AIM 3: To explore the role of early experiences with ethanol in mothers' milk and the emotional context of such experiences, on the recognition of, and preference for, the sensory properties of ethanol ..
  12. REDOX PROPERTIES OF FLAVODOXIN-LIKE FLAVOPROTEINS
    RICHARD SWENSON; Fiscal Year: 2007
    ..These studies are also designed to expand our understanding of the fundamentally different way that the one electron reduced state (the flavin semiquinone) is stabilized and is utilized in these two different systems. ..
  13. Epidemiology of an uropathogenic E coli clonal group
    Lee Riley; Fiscal Year: 2003
    ..coli can be spread by contaminated ingestible vehicles, and use the data generated from this project to design long-term studies to better characterize risk factors associated with CgA infection. ..
  14. Flow Cytometry to Detect Human Tumor Cells in Body Fluid
    AWTAR GANJU KRISHAN; Fiscal Year: 2003
    ..3. Compare data collected from flow cytometric analysis with that obtained from cytopathology and immunocytochemistry routinely performed on these specimens for diagnostic purpose. ..
  15. F1-ATPase Chemical-Mechanical Coupling Mechanisms
    Wayne D Frasch; Fiscal Year: 2010
    ..abstract_text> ..
  16. NOVEL E. COLI 0157:H7 INTESTINAL COLONIZATION FACTORS
    JAMES KAPER; Fiscal Year: 2009
    ....
  17. DEVELOPMENT OF A LIVE ORAL CHOLERA VACCINE
    James B Kaper; Fiscal Year: 2010
    ..Lay summary: Vibrio cholerae is a bacterium that causes cholera, a severe diarrheal disease that can often cause death. This project will develop improved vaccines for the prevention of cholera. ..
  18. MOLECULAR GENETICS OF ENTEROPATHOGENIC E COLI ADHESION
    JAMES KAPER; Fiscal Year: 2003
    ..The proposed experimental approach will use a combination of molecular genetics, cell biology, and animal studies to achieve a better understanding of how EPEC infects intestinal epithelial cells and causes disease. ..