Summary: An individual that contains cell populations derived from different zygotes.

Top Publications

  1. ncbi Hepatitis C virus replication in mice with chimeric human livers
    D F Mercer
    Surgical-Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Nat Med 7:927-33. 2001
  2. pmc Germline competent embryonic stem cells derived from rat blastocysts
    Ping Li
    Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Cell 135:1299-310. 2008
  3. pmc Mechanism of chimera formation during the Multiple Displacement Amplification reaction
    Roger S Lasken
    J Craig Venter Institute, Rockville, MD 20850, USA
    BMC Biotechnol 7:19. 2007
  4. pmc DECIPHER, a search-based approach to chimera identification for 16S rRNA sequences
    Erik S Wright
    Department of Civil and Environmental Engineering, University of Wisconsin Madison, Madison, Wisconsin, USA
    Appl Environ Microbiol 78:717-25. 2012
  5. ncbi In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
    Marius Wernig
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 448:318-24. 2007
  6. ncbi Incompatibility of nuclear and mitochondrial genomes causes hybrid sterility between two yeast species
    Hsin Yi Lee
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    Cell 135:1065-73. 2008
  7. ncbi Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors
    Jeong Beom Kim
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Munster, NRW, Germany
    Nature 454:646-50. 2008
  8. ncbi Brief report: chimeric pigs produced from induced pluripotent stem cells demonstrate germline transmission and no evidence of tumor formation in young pigs
    Franklin D West
    Regenerative Bioscience Center, University of Georgia, Athens, Georgia, USA
    Stem Cells 29:1640-3. 2011
  9. pmc Transposable element proliferation and genome expansion are rare in contemporary sunflower hybrid populations despite widespread transcriptional activity of LTR retrotransposons
    Takeshi Kawakami
    Division of Biology, Kansas State University, USA
    Genome Biol Evol 3:156-67. 2011
  10. ncbi Validated germline-competent embryonic stem cell lines from nonobese diabetic mice
    Jennifer Nichols
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK
    Nat Med 15:814-8. 2009

Detail Information

Publications276 found, 100 shown here

  1. ncbi Hepatitis C virus replication in mice with chimeric human livers
    D F Mercer
    Surgical-Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Nat Med 7:927-33. 2001
    ..These chimeric mice represent the first murine model suitable for studying the human hepatitis C virus in vivo...
  2. pmc Germline competent embryonic stem cells derived from rat blastocysts
    Ping Li
    Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Cell 135:1299-310. 2008
    ..Establishment of authentic rat ES cells will make possible sophisticated genetic manipulation to create models for the study of human diseases...
  3. pmc Mechanism of chimera formation during the Multiple Displacement Amplification reaction
    Roger S Lasken
    J Craig Venter Institute, Rockville, MD 20850, USA
    BMC Biotechnol 7:19. 2007
    ..Determining the source of the DNA rearrangements would be an important step towards reducing or eliminating them...
  4. pmc DECIPHER, a search-based approach to chimera identification for 16S rRNA sequences
    Erik S Wright
    Department of Civil and Environmental Engineering, University of Wisconsin Madison, Madison, Wisconsin, USA
    Appl Environ Microbiol 78:717-25. 2012
    ..Overall, ss_DECIPHER and Uchime provided the highest chimera detection for sequences 100 to 600 nucleotides long (79% and 81%, respectively), but Uchime's performance ..
  5. ncbi In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
    Marius Wernig
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 448:318-24. 2007
    ..Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells...
  6. ncbi Incompatibility of nuclear and mitochondrial genomes causes hybrid sterility between two yeast species
    Hsin Yi Lee
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    Cell 135:1065-73. 2008
    ..bayanus Aep2 protein to regulate the translation of the S. cerevisiae OLI1 mRNA. We speculate that AEP2 and OLI1 have evolved during the adaptation of S. bayanus to nonfermentable carbon sources, thereby driving speciation...
  7. ncbi Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors
    Jeong Beom Kim
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Munster, NRW, Germany
    Nature 454:646-50. 2008
    ..We propose that, in inducing pluripotency, the number of reprogramming factors can be reduced when using somatic cells that endogenously express appropriate levels of complementing factors...
  8. ncbi Brief report: chimeric pigs produced from induced pluripotent stem cells demonstrate germline transmission and no evidence of tumor formation in young pigs
    Franklin D West
    Regenerative Bioscience Center, University of Georgia, Athens, Georgia, USA
    Stem Cells 29:1640-3. 2011
  9. pmc Transposable element proliferation and genome expansion are rare in contemporary sunflower hybrid populations despite widespread transcriptional activity of LTR retrotransposons
    Takeshi Kawakami
    Division of Biology, Kansas State University, USA
    Genome Biol Evol 3:156-67. 2011
  10. ncbi Validated germline-competent embryonic stem cell lines from nonobese diabetic mice
    Jennifer Nichols
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK
    Nat Med 15:814-8. 2009
    ..The availability of these cells will not only enable the dissection of closely linked loci and the role they have in the onset of type 1 diabetes but also facilitate the generation of new transgenics...
  11. ncbi Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells
    R L Williams
    European Molecular Biology Laboratory, Heidelberg, FRG
    Nature 336:684-7. 1988
    ..We demonstrate here that purified, recombinant LIF can substitute for DIA in the maintenance of totipotent ES cell lines that retain the potential to form chimaeric mice...
  12. pmc Near completely humanized liver in mice shows human-type metabolic responses to drugs
    Chise Tateno
    Yoshizato Project, Cooperative Link of Unique Science and Technology for Economy Revitalization CLUSTER, Hiroshima, Japan
    Am J Pathol 165:901-12. 2004
    ..We conclude that the chimeric mouse developed in the present study is a useful model for assessing the functions and pharmacological responses of human hepatocytes...
  13. ncbi Porcine induced pluripotent stem cells produce chimeric offspring
    Franklin D West
    Regenerative Bioscience Center, University of Georgia, Athens, Georgia 30602, USA
    Stem Cells Dev 19:1211-20. 2010
    Ethical and moral issues rule out the use of human induced pluripotent stem cells (iPSCs) in chimera studies that would determine the full extent of their reprogrammed state, instead relying on less rigorous assays such as teratoma ..
  14. ncbi Generation of rat pancreas in mouse by interspecific blastocyst injection of pluripotent stem cells
    Toshihiro Kobayashi
    Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Cell 142:787-99. 2010
    ..These data constitute proof of principle for interspecific blastocyst complementation and for generation in vivo of organs derived from donor PSCs using a xenogenic environment...
  15. pmc Mice chronically infected with chimeric HIV resist peripheral and brain superinfection: a model of protective immunity to HIV
    Molecular Virology Division, St Luke s Roosevelt Hospital Center, 432 West 58th Street, New York, NY 10019, USA
    J Neuroimmune Pharmacol 7:380-7. 2012
    ..These findings suggest EcoHIV-infected mice may serve as a useful system to investigate the induction of anti-HIV protective immunity for eventual translation to human beings...
  16. pmc Generation of chimeric rhesus monkeys
    Masahito Tachibana
    Oregon National Primate Research Center, Oregon Health and Science University, 505 N W 185 th Avenue, Beaverton, OR 97006, USA
    Cell 148:285-95. 2012
    ..These results provide insights into the species-specific nature of primate embryos and suggest that a chimera assay using pluripotent cells may not be feasible.
  17. pmc Germline transmission of a novel rat embryonic stem cell line derived from transgenic rats
    Hongsheng Men
    Department of Veterinary Pathobiology, Rat Resource and Research Center, University of Missouri, Columbia, Missouri 65201, USA
    Stem Cells Dev 21:2606-12. 2012
  18. ncbi The origin and fate of yolk sac hematopoiesis: application of chimera analyses to developmental studies
    Hiroo Ueno
    Institute of Stem Cell Biology and Regenerative Medicine, Department of Pathology, Ludwig Institute at Stanford University, Stanford University, Stanford, CA, USA
    Int J Dev Biol 54:1019-31. 2010
    ..In this review, we will survey the state of our current knowledge in these areas, and discuss the potential use of multicolor chimera analyses to elucidate unresolved questions.
  19. ncbi Matrix protein and glycoproteins F and H of Peste-des-petits-ruminants virus function better as a homologous complex
    M Mahapatra
    Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, UK
    J Gen Virol 87:2021-9. 2006
    ..Therefore, in an effort to improve the growth of the RPV-PPRFH virus, a triple chimera (RPV-PPRMFH) was made, where the M, F and H genes of RPV were replaced with those from PPRV...
  20. ncbi Differences in transcription levels among wild, domesticated, and hybrid Atlantic salmon (Salmo salar) from two environments
    Paul V Debes
    Department of Biology, Dalhousie University, LSC, 1355 Oxford Road, Halifax, NS B3H 4J1, Canada
    Mol Ecol 21:2574-87. 2012
  21. ncbi Genome-wide transcript analysis of maize hybrids: allelic additive gene expression and yield heterosis
    Mei Guo
    Pioneer Hi Bred International, Inc, 7250 NW 62nd Avenue, Johnston, IA 50131, USA
    Theor Appl Genet 113:831-45. 2006
    ..Our findings suggest that differential allele regulation may play an important role in hybrid yield or heterosis, and provide a new insight to the molecular understanding of the underlying mechanisms of heterosis...
  22. pmc Behavioral and spermatogenic hybrid male breakdown in Nasonia
    M E Clark
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Heredity (Edinb) 104:289-301. 2010
  23. ncbi Effects of temperature, pH and sugar concentration on the growth parameters of Saccharomyces cerevisiae, S. kudriavzevii and their interspecific hybrid
    F Noé Arroyo-López
    Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Edifici d Instituts, Parc Científic de Paterna, P O Box 22085, E 46071 Valencia, Spain
    Int J Food Microbiol 131:120-7. 2009
    ..The information obtained could also be useful to estimate the possible effect of climatic change on yeast growth...
  24. ncbi Teratoma formation assays with human embryonic stem cells: a rationale for one type of human-animal chimera
    M William Lensch
    Division of Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell Stem Cell 1:253-8. 2007
    Despite a long and valuable history, human-animal chimera research has often been questioned...
  25. pmc Allelic variation of gene expression in maize hybrids
    Mei Guo
    Pioneer Hi Bred International, Johnston, Iowa 50131, USA
    Plant Cell 16:1707-16. 2004
    ..The results of allele-specific regulation in different tissues in responding to environment and stress suggest an unequivalent function of the parental alleles in the hybrid, which may have an impact on heterosis...
  26. ncbi HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells
    M Hooper
    Nature 326:292-5. 1987
    ..In human males, HPRT deficiency causes Lesch-Nyhan syndrome, which is characterized by mental retardation and self-mutilation...
  27. ncbi Influence of hepatitis B virus genotypes on the intra- and extracellular expression of viral DNA and antigens
    Masaya Sugiyama
    Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
    Hepatology 44:915-24. 2006
    ..In conclusion, virological differences among HBV genotypes were demonstrated both in vitro and in vivo. These differences may influence HBV infections with distinct genotypes in clinical and epidemiological settings...
  28. pmc Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development
    Akio Kobayashi
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Cell Stem Cell 3:169-81. 2008
    ..Taken together, our observations suggest that Six2 activity cell-autonomously regulates a multipotent nephron progenitor population...
  29. ncbi Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse
    G Winnier
    Howard Hughes Medical Institute, Vanderbilt University Medical School, Nashville, Tennessee 37232 2175, USA
    Genes Dev 9:2105-16. 1995
    ..Moreover, in the presumed absence of zygotic ligand, it appears that homozygous mutants can be rescued partially by related proteins or by maternal BMP-4...
  30. pmc Changes in 24-nt siRNA levels in Arabidopsis hybrids suggest an epigenetic contribution to hybrid vigor
    Michael Groszmann
    Commonwealth Scientific and Industrial Research Organization Plant Industry, Canberra, Australian Capital Territory 2601, Australia
    Proc Natl Acad Sci U S A 108:2617-22. 2011
  31. ncbi Capture of authentic embryonic stem cells from rat blastocysts
    Mia Buehr
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, Edinburgh EH93JQ, UK
    Cell 135:1287-98. 2008
    ..Rat ES cells will also provide a refined test-bed for functional evaluation of pluripotent stem cell-derived tissue repair and regeneration...
  32. pmc Efavirenz therapy in rhesus macaques infected with a chimera of simian immunodeficiency virus containing reverse transcriptase from human immunodeficiency virus type 1
    Michael J Hofman
    Center for Comparative Medicine, University of California, Davis, CA 95616, USA
    Antimicrob Agents Chemother 48:3483-90. 2004
    ..However, a SIV-HIV-1 chimera (RT-SHIV), in which the RT from SIVmac239 was replaced with the RT-encoding region from HIV-1, is susceptible to ..
  33. pmc Dendritic cells require a systemic type I interferon response to mature and induce CD4+ Th1 immunity with poly IC as adjuvant
    M Paula Longhi
    Laboratory of Cellular Physiology and Immunology and Chris Browne Center, The Rockefeller University, New York, NY 10065, USA
    J Exp Med 206:1589-602. 2009
    ..Therefore, the adjuvant action of poly IC requires a widespread innate type I IFN response that directly links antigen presentation by DCs to adaptive immunity...
  34. pmc Ligand-binding domain of an α7-nicotinic receptor chimera and its complex with agonist
    Shu Xing Li
    Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, California, USA
    Nat Neurosci 14:1253-9. 2011
    ..We determined the crystal structure of the extracellular domain of a receptor chimera constructed from the human α(7) AChR and Lymnaea stagnalis acetylcholine binding protein (AChBP), which shares ..
  35. pmc Granulocyte colony-stimulating factor induces osteoblast apoptosis and inhibits osteoblast differentiation
    Matthew J Christopher
    Division of Oncology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Bone Miner Res 23:1765-74. 2008
    ..In sum, these findings suggest that the hematopoietic system may play a novel role in regulating osteoblast differentiation and apoptosis during G-CSF treatment...
  36. ncbi The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo
    J Martin Collinson
    Division of Reproductive and Developmental Sciences, Genes and Development Group, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh EH8 9XD, Scotland, UK
    Dev Biol 255:303-12. 2003
  37. ncbi Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells
    Shogo Nagata
    Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Genes Cells 14:1395-404. 2009
  38. ncbi Epigenetic, transposon and small RNA determinants of hybrid dysfunctions
    P Michalak
    Department of Biology, The University of Texas Arlington, Arlington, TX 76019 0498, USA
    Heredity (Edinb) 102:45-50. 2009
    ..The review offers a handful of glimpses into these complex dynamics...
  39. pmc Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells
    Sarah Eminli
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Boston, Massachusetts, USA
    Nat Genet 41:968-76. 2009
    ..Moreover, we identify hematopoietic progenitors as an attractive cell type for applications of iPS cell technology in research and therapy...
  40. ncbi Altered imprinted gene methylation and expression in completely ES cell-derived mouse fetuses: association with aberrant phenotypes
    W Dean
    Laboratory of Developmental Genetics Programme, The Babraham Institute, Babraham Hall, Cambridge CB2 4AT, UK
    Development 125:2273-82. 1998
    ..Our study establishes a model for how early embryonic epigenetic alterations in imprinted genes persist to later developmental stages, and are associated with aberrant phenotypes...
  41. ncbi Different scales of Ty1/copia-like retrotransposon proliferation in the genomes of three diploid hybrid sunflower species
    T Kawakami
    Division of Biology, Kansas State University, Manhattan, KS 66506, USA
    Heredity (Edinb) 104:341-50. 2010
    ..These findings indicate that the genomic and/or environmental conditions associated with the origins of these sunflower hybrid taxa were conducive to derepression of at least two major groups of transposable elements...
  42. ncbi Infection of human hepatocyte chimeric mouse with genetically engineered hepatitis B virus
    Masataka Tsuge
    Department of Medicine and Molecular Science, Division of Frontier Medical Science, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, Minami Ku, Hiroshima shi, Japan
    Hepatology 42:1046-54. 2005
    ..In conclusion, this mouse model of HBV infection is a useful tool for the study of HBV virology and evaluation of anti-HBV drugs. Our results indicate that HBeAg is dispensable for active viral production and transmission...
  43. ncbi The role of TNF-alpha in the pathogenesis of inflammation and joint destruction in rheumatoid arthritis (RA): a study using a human RA/SCID mouse chimera
    H Matsuno
    Department of Orthopedic Surgery, Toyama Medical and Pharmaceutical University, Toyama, UK
    Rheumatology (Oxford) 41:329-37. 2002
  44. ncbi Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb
    Bo Feng
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, 02 01, Genome Building, Singapore 138672
    Nat Cell Biol 11:197-203. 2009
    ..Our findings also indicate that it is possible to reprogram MEFs without exogenous Klf transcription factors and link a nuclear receptor to somatic cell reprogramming...
  45. ncbi Mapping the receptor site for hanatoxin, a gating modifier of voltage-dependent K+ channels
    K J Swartz
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Neuron 18:675-82. 1997
    ..These results suggest that residues near the outer edges of S3 and S4 form the HaTx binding sites and are eccentrically located at least 15 A from the central pore axis on the surface of voltage-gated K+ channels...
  46. pmc The rate of genome stabilization in homoploid hybrid species
    C Alex Buerkle
    Department of Botany, 1000 E University Ave, University of Wyoming, Laramie, Wyoming 82071, USA
    Evolution 62:266-75. 2008
    ..Our modeling of genome stabilization provides an upper bound for the time interval for reproductive isolation to be established and confirms the rapid nature of homoploid hybrid speciation...
  47. ncbi Multiple functions for Pax6 in mouse eye and nasal development
    J C Quinn
    Department of Obstetrics and Gynecology, University of Edinburgh, UK
    Genes Dev 10:435-46. 1996
    ..We suggest that Pax6 may promote cell surface changes in the optic cup and control the fate of the ectoderm from which the lens and nasal epithelia are derived...
  48. pmc Analysis of BAC-end sequences (BESs) and development of BES-SSR markers for genetic mapping and hybrid purity assessment in pigeonpea (Cajanus spp.)
    Abhishek Bohra
    International Crops Research Institute for the Semi Arid Tropics ICRISAT, Patancheru, Hyderabad, 502324, India
    BMC Plant Biol 11:56. 2011
  49. ncbi Derivation of embryonic stem cells from Brown Norway rats blastocysts
    Xiaoyang Zhao
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    J Genet Genomics 37:467-73. 2010
    ..Our success in generating pluripotent rat ES cells will benefit the generation of the knockout rats in the future...
  50. pmc Macrophage are the principal reservoir and sustain high virus loads in rhesus macaques after the depletion of CD4+ T cells by a highly pathogenic simian immunodeficiency virus/HIV type 1 chimera (SHIV): Implications for HIV-1 infections of humans
    T Igarashi
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0460, USA
    Proc Natl Acad Sci U S A 98:658-63. 2001
    ..They become infected during the acute infection, gradually increase in number over time, and can be a major contributor to total body virus burden during the symptomatic phase of the human infection...
  51. ncbi A phylogeographic investigation of the hybrid origin of a species of swordtail fish from Mexico
    Julia C Jones
    Lehrstuhl fur Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitatstrasse 10, 78457 Konstanz, Germany
    Mol Ecol 21:2692-712. 2012
    ..clemenciae and X. helleriii and provide preliminary information to aid in conservation management of this geographically restricted hybrid species, X. clemenciae...
  52. pmc Genetic dissection of a key reproductive barrier between nascent species of house mice
    Michael A White
    Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706 1580, USA
    Genetics 189:289-304. 2011
    ..domesticus autosomal alleles. Our results reveal a complex genetic architecture for hybrid male sterility and suggest a prominent role for reproductive barriers in advanced generations in maintaining subspecies integrity in house mice...
  53. ncbi FcR gamma chain deletion results in pleiotrophic effector cell defects
    T Takai
    DeWitt Wallace Research Laboratory, Sloan Kettering Institute, New York, New York 10021
    Cell 76:519-29. 1994
    ..These mice thus represent an important tool for evaluating the role of these receptors in humoral and cellular immune responses...
  54. ncbi Sex differentiation and germ cell production in chimeric pigs produced by inner cell mass injection into blastocysts
    Hiroshi Nagashima
    Reproductive Biology Division, BresaGen Limited, Rundle Mall, Adelaide, South Australia 5000, Australia
    Biol Reprod 70:702-7. 2004
    ..XX donor cells were injected into XY blastocysts at the same embryonic stage, the phenotypic sex of the resulting chimera was male with no germ-line cells formed from the donor cell lineage...
  55. ncbi Wheat (Triticum aestivum L.) root proteome and differentially expressed root proteins between hybrid and parents
    Xiao Song
    Key Laboratory of Crop Heterosis and Utilization, China Agricultural University, Beijing, China
    Proteomics 7:3538-57. 2007
    ..These results indicated that hybridization between two parental lines can cause expression differences between wheat hybrid and its parents not only at mRNA levels but also at protein abundances...
  56. ncbi Production of loach (Misgurnus anguillicaudatus) germ-line chimera using transplantation of primordial germ cells isolated from cryopreserved blastomeres
    G S Yasui
    Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Sciences, Graduate School of Fisheries Sciences, Hokkaido University, 3 1 1, Hakodate, Hokkaido, 041 8611, Japan
    J Anim Sci 89:2380-8. 2011
    An efficient procedure for the cryopreservation of fish blastomeres followed by restoration through germ-line chimera formation was established...
  57. ncbi Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
    ..These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required...
  58. pmc Contrasting patterns of selective constraints in nuclear-encoded genes of the oxidative phosphorylation pathway in holometabolous insects and their possible role in hybrid breakdown in Nasonia
    J D Gibson
    School of Life Sciences, Arizona State University, Tempe, AZ 85287 4501, USA
    Heredity (Edinb) 104:310-7. 2010
    ..It is possible that these genes account for the reported incompatibility in Nasonia hybrids and their characterization may lead to a better understanding of the role of positive selection in the genetics of speciation...
  59. pmc Tbx3 improves the germ-line competency of induced pluripotent stem cells
    Jianyong Han
    Stem Cell and Developmental Biology, Genome Institute of Singapore, 138672, Singapore
    Nature 463:1096-100. 2010
    ..This study underscores the intrinsic qualitative differences between iPS cells generated by different methods, and highlights the need to rigorously characterize iPS cells beyond in vitro studies...
  60. ncbi Defective lymphoid development in mice lacking Jak3
    T Nosaka
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Science 270:800-2. 1995
    ..Thus, Jak3 plays a critical role in gamma c signaling and lymphoid development...
  61. pmc Interordinal chimera formation between medaka and zebrafish for analyzing stem cell differentiation
    Ni Hong
    Department of Biological Sciences, National University of Singapore, Singapore
    Stem Cells Dev 21:2333-41. 2012
    b>Chimera formation is a standard test for pluripotency of stem cells in vivo. Interspecific chimera formation between distantly related organisms offers also an attractive approach for propagating endangered species...
  62. ncbi NMR metabolomics of thrips (Frankliniella occidentalis) resistance in Senecio hybrids
    Kirsten A Leiss
    Institute of Biology, Leiden University, The Netherlands
    J Chem Ecol 35:219-29. 2009
    ..It constitutes a significant advance in the study of plant-insect relationships, providing key information on the implementation of herbivore resistance breeding strategies in plants...
  63. ncbi Assortative mating preferences among hybrids offers a route to hybrid speciation
    Maria C Melo
    Instituto de Genetica, Universidad de Los Andes, Carrera 1E No 18 10, P O Box 4976, Santafé de Bogotá D C, Colombia
    Evolution 63:1660-5. 2009
    ..Second, once established such a lineage would inherit assortative mating preferences that would lead to partial reproductive isolation from parental lineages...
  64. ncbi Rapid loss of Oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines
    M Buehr
    Centre for Genome Research, University of Edinburgh, Edinburgh EH9 3JQ, United Kingdom
    Biol Reprod 68:222-9. 2003
    ..Downregulation of Oct-4 appears to be a limiting factor in attempts to derive pluripotent cell lines from preimplantation embryos...
  65. ncbi Chimeric mouse tumor models reveal differences in pathway activation between ERBB family- and KRAS-dependent lung adenocarcinomas
    Yinghui Zhou
    1 AVEO Pharmaceuticals, Cambridge, Massachusetts, USA 2 These authors contributed equally to this work
    Nat Biotechnol 28:71-8. 2010
    ..for generating mouse tumor models that involves stepwise genetic manipulation of embryonic stem (ES) cells and chimera generation. Tumors in the chimeric animals develop from engineered cells in the context of normal tissue...
  66. ncbi Testing antiretroviral drug efficacy in conventional mice infected with chimeric HIV-1
    Eran Hadas
    Molecular Virology Division, St Luke s Roosevelt Hospital Center, Columbia University Medical Center, New York 10019, USA
    AIDS 21:905-9. 2007
    ..We have now applied this system as a model for preclinical evaluation of anti-retroviral drugs...
  67. pmc Challenge pools of hepatitis C virus genotypes 1-6 prototype strains: replication fitness and pathogenicity in chimpanzees and human liver-chimeric mouse models
    Jens Bukh
    Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg 50, 50 S Dr MSC 8009, Bethesda, MD 20892 8009, USA
    J Infect Dis 201:1381-9. 2010
    ..The challenge pools represent a unique resource for studies of HCV molecular virology and for studies of pathogenesis, protective immunity, and vaccine efficacy in vivo...
  68. pmc A human-mouse chimera of the alpha3alpha4alpha5(IV) collagen protomer rescues the renal phenotype in Col4a3-/- Alport mice
    Laurence Heidet
    Inserm U574, Hopital Necker Enfants Malades, Universite Rene Descartes, Paris, France
    Am J Pathol 163:1633-44. 2003
    ..This line of mice, humanized for the alpha3(IV) collagen chain, will also provide a valuable model for studying the pathogenesis of Goodpasture syndrome, an autoimmune disease caused by antibodies against this chain...
  69. ncbi The chemokine receptor CXCR4 is required for the retention of B lineage and granulocytic precursors within the bone marrow microenvironment
    Q Ma
    The Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Immunity 10:463-71. 1999
    ..Granulocytic cells are reduced in bone marrow but elevated and less mature in the blood. B lineage and granulocytic precursors are released into the periphery in absence of CXCR4...
  70. pmc Critical role of phospholipase Cgamma2 in integrin and Fc receptor-mediated neutrophil functions and the effector phase of autoimmune arthritis
    Zoltan Jakus
    Department of Physiology, Semmelweis University School of Medicine, 1094 Budapest, Hungary
    J Exp Med 206:577-93. 2009
    ..These results identify PLCgamma2 as a critical player of integrin and Fc receptor-mediated neutrophil functions and the neutrophil-mediated effector phase of autoimmune arthritis...
  71. ncbi Defective T-cell receptor signalling and positive selection of Vav-deficient CD4+ CD8+ thymocytes
    K D Fischer
    Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Nature 374:474-7. 1995
  72. ncbi The calpain small subunit gene is essential: its inactivation results in embryonic lethality
    U J Zimmerman
    Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104 6068, USA
    IUBMB Life 50:63-8. 2000
    ..The results indicate that at least one normal allele is essential for postnatal survival. Disruption of both alleles appears to be lethal in very early fetal development...
  73. pmc Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro
    Brett D Lindenbach
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:3805-9. 2006
    ..These results greatly extend the utility of the HCVcc genetic system to allow the complete in vitro and in vivo dissection of the HCV life cycle...
  74. pmc Primary defects in the lens underlie complex anterior segment abnormalities of the Pax6 heterozygous eye
    J M Collinson
    Department of Reproductive and Developmental Sciences and Department of Biomedical Sciences, Genes and Development Group, University of Edinburgh, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 98:9688-93. 2001
    ..Therefore, these aspects of the phenotype may be secondary consequences of primary defects in the lens, which has clinical relevance for the human aniridia (PAX6(+/-)) phenotype...
  75. ncbi Wild-type nonneuronal cells extend survival of SOD1 mutant motor neurons in ALS mice
    A M Clement
    Ludwig Institute for Cancer Research, University of California, 9500 Gilman Drive, La Jolla, CA 92093 0670, USA
    Science 302:113-7. 2003
    ..Normal motor neurons in SOD1 mutant chimeras develop aspects of ALS pathology. Most important, nonneuronal cells that do not express mutant SOD1 delay degeneration and significantly extend survival of mutant-expressing motor neurons...
  76. ncbi Diversification within grapevine cultivars goes through chimeric states
    S Hocquigny
    Laboratoire de Génétique et d Amélioration des Plantes, Unité Mixte de Recherche 1131 Institut National de la Recherche Agronomique, Universite Louis Pasteur, Colmar, France
    Genome 47:579-89. 2004
    ..A hypothesis explaining the triallelic profiles at a locus is the presence of a periclinal chimera meristem structure, in which genetically different cell layers coexist...
  77. ncbi Prediction of hybrid performance in grain sorghum using RFLP markers
    D R Jordan
    Department of Primary Industries, Hermitage Research Station, Warwick, Queensland 4370, Australia
    Theor Appl Genet 106:559-67. 2003
    ..A model combining phenotypic trait data and parental diversity on particular linkage groups explained 71% of the variation in grain yield and has potential for use in the selection of heterotic hybrids...
  78. ncbi Germ-line transmission of a c-abl mutation produced by targeted gene disruption in ES cells
    P L Schwartzberg
    Department of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians and Surgeons, New York, NY 10032
    Science 246:799-803. 1989
    ..This work demonstrates the feasibility of germ-line transmission of a mutation introduced into a nonselectable autosomal gene by homologous recombination...
  79. ncbi Mice lacking the tumour necrosis factor receptor 1 are resistant to TNF-mediated toxicity but highly susceptible to infection by Listeria monocytogenes
    J Rothe
    Department of Biology, Hoffmann La Roche Ltd, Basel, Switzerland
    Nature 364:798-802. 1993
    ..The increased susceptibility of Tnfr1(0)/Tnfr1(0) mutant mice to infection with the facultative intracellular bacterium Listeria monocytogenes indicates an essential role of TNF in nonspecific immunity...
  80. ncbi Stable expression and characterisation of a human alpha 7 nicotinic subunit chimera: a tool for functional high-throughput screening
    Peter J Craig
    Eli Lilly and Company Ltd, Lilly Research Centre, Erl Wood Manor, Windlesham, Surrey GU20 6PH, UK
    Eur J Pharmacol 502:31-40. 2004
    A chimera comprising the N-terminal region of the human alpha7 nicotinic acetylcholine receptor, fused to the transmembrane/C-terminal domains of the mouse serotonin 5-HT3 receptor, was constructed...
  81. ncbi Activation of bacterial porin gene expression by a chimeric signal transducer in response to aspartate
    R Utsumi
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854
    Science 245:1246-9. 1989
    ..The activation of ompC by the chimeric receptor was absolutely dependent on OmpR, a transcriptional activator for ompF and ompC...
  82. ncbi Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB Brassica interspecific hybrids
    Annaliese S Mason
    School of Plant Biology and Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia
    Chromosome Res 18:655-66. 2010
    ..juncea × B. napus) hybrid types. Homoeologous pairing between the Brassica A, B and C genomes in interspecific hybrids may be influenced by complex interactions between genome structure and allelic composition...
  83. ncbi Fitness and maternal effects in hybrids formed between transgenic oilseed rape (Brassica napus L.) and wild brown mustard [B. juncea (L.) Czern et Coss.] in the field
    Kun Di
    State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China
    Pest Manag Sci 65:753-60. 2009
    ..The primary aim of this study was to compare the fitness of transgenic crop x wild hybrids with their parental types and a non-transgenic crop type in the field under enhanced temperature and humidity...
  84. pmc Reprogramming of pancreatic beta cells into induced pluripotent stem cells
    Matthias Stadtfeld
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA
    Curr Biol 18:890-4. 2008
    ..Our results provide genetic proof that terminally differentiated cells can be reprogrammed into pluripotent cells, suggesting that in vitro reprogramming is not restricted to certain cell types or differentiation stages...
  85. pmc Genetic evidence for hybrid trait speciation in heliconius butterflies
    Camilo Salazar
    Department of Zoology, University of Cambridge, Cambridge, United Kingdom
    PLoS Genet 6:e1000930. 2010
    ..This gene therefore constitutes the first molecular evidence for adaptive introgression during hybrid speciation and is the first clear candidate for a Heliconius wing patterning locus...
  86. ncbi Roles of deletion and regulation in creating mixed chimerism and allograft tolerance using a nonlymphoablative irradiation-free protocol
    Christoph Domenig
    Transplantation Research Center, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    J Immunol 175:51-60. 2005
    ..In this model, both deletional and immunoregulatory mechanisms are active during the induction and/or maintenance of allograft tolerance through creation of MC using a potentially clinically applicable regimen...
  87. pmc Local adaptation within a hybrid species
    F Eroukhmanoff
    Centre for Ecological and Evolutionary Synthesis CEES, Department of Biology, University of Oslo, Oslo, Norway
    Heredity (Edinb) 111:286-92. 2013
    ..The study illustrates how hybrid species may be relatively unconstrained by their admixed genetic background, allowing them to adapt rapidly to environmental variation. ..
  88. ncbi Involvement of distinct cellular compartments in the abnormal lymphoid organogenesis in lymphotoxin-alpha-deficient mice and alymphoplasia (aly) mice defined by the chimeric analysis
    M Matsumoto
    First Department of Internal Medicine, School of Medicine, Ehime University, Japan
    J Immunol 163:1584-91. 1999
  89. ncbi Induction of the mammalian node requires Arkadia function in the extraembryonic lineages
    V Episkopou
    Mammalian Neurogenesis, MRC Clinical Science Centre, Imperial College School of Medicine, Hammersmith Hospital, London, UK
    Nature 410:825-30. 2001
    ..Furthermore, our experiments show that Arkadia interacts genetically with the transforming growth factor (TGF)beta-like factor Nodal, implying that Nodal mediates the function of Arkadia in node induction...
  90. pmc A chimera analysis of prestin knock-out mice
    Mary Ann Cheatham
    Departments of Communication Sciences and Disorders, The Hugh Knowles Center, Northwestern University, Evanston, Illinois 60208, USA
    J Neurosci 29:12000-8. 2009
    A chimera is a genetic composite containing a unique mix of cells derived from more than one zygote. This mouse model allows one to learn how cells of contrasting genotype functionally interact in vivo...
  91. ncbi Corneal development, limbal stem cell function, and corneal epithelial cell migration in the Pax6(+/-) mouse
    J Martin Collinson
    Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, United Kingdom
    Invest Ophthalmol Vis Sci 45:1101-8. 2004
    ..To investigate the etiology of corneal dysfunction in the Pax6(+/-) mouse model of aniridia-related keratopathy...
  92. pmc Accessing naïve human pluripotency
    Alejandro De Los Angeles
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Children s Hospital Boston, MA 02115, USA
    Curr Opin Genet Dev 22:272-82. 2012
    ..Unraveling how to stably induce naïve pluripotency in human cells will influence the full realization of human pluripotent stem cell biology and medicine...
  93. ncbi Chimera and other fertilization errors
    V Malan
    Service de Cytogénétique, Hopital Necker Enfants Malades, APHP, Paris, René Descartes Paris 5 University, Paris, France
    Clin Genet 70:363-73. 2006
    ..It may be the result of mosaicism or chimerism. By definition, a chimera is produced by the fusion of two different zygotes in a single embryo, while a mosaic contains genetically ..
  94. pmc Genetic analysis of alpha 4 integrin functions in the development of mouse skeletal muscle
    J T Yang
    Howard Hughes Medical Institute Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
    J Cell Biol 135:829-35. 1996
    ..Taking these results together, we conclude that alpha 4 integrins appear not to play essential roles in normal skeletal muscle development...
  95. ncbi Rare and asymmetrical hybridization of the endemic Barbus carpathicus with its widespread congener Barbus barbus
    Z Lajbner
    Department of Vertebrate Evolutionary Biology and Genetics, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburska 89, 277 21 Libechov, Czech Republic
    J Fish Biol 74:418-36. 2009
    ..barbus mothers. Despite geographically broad occurrence of hybrids, these data provide evidence of strong constraints on hybridization in the native breeding habitats and the lack of introgression towards B. carpathicus...
  96. pmc Glucocorticoid-mediated repression of cytokine gene transcription in human arteritis-SCID chimeras
    A Brack
    Department of Medicine, Division of Rheumatology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
    J Clin Invest 99:2842-50. 1997
    ..The persistence of TGF-beta1-transcribing macrophages, despite paralysis of T cell function, may provide an explanation for the chronicity of the disease, and may identify a novel therapeutic target in this inflammatory vasculopathy...
  97. ncbi Dispermic chimerism with two abnormal cell lines, 47,XY, +21 and 47,XX, +12
    John E Wiley
    Department of Pediatrics, The Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    Am J Med Genet 107:64-6. 2002
    ..The difference in sex chromosome constitution between the cell lines indicated that the fetus had dispermic chimerism. To our knowledge, this is the first report of chimerism with two abnormal cell lines...
  98. ncbi Generation of transgenic porcine chimeras using primordial germ cell-derived colonies
    J A Piedrahita
    Department of Veterinary Anatomy and Public Health, Center for Animal Biotechnology, Texas A and M University, College Station 77843 4458, USA
    Biol Reprod 58:1321-9. 1998
    ..Fluorescent colonies were selected for chimera generation...
  99. ncbi Analysis of mouse eye development with chimeras and mosaics
    J Martin Collinson
    School of Medical Sciences, University of Aberdeen, Scotland, UK
    Int J Dev Biol 48:793-804. 2004
    ..The uses of chimeras, Cre-loxP mosaics and in vitro tissue recombination for study of ocular organogenesis are compared. Wider use of mosaics and chimeras should provide further insights into eye development...
  100. ncbi Development of a pheasant interspecies primordial germ cell transfer to chicken embryo: effect of donor cell sex on chimeric semen production
    S J Kang
    WCU Biomodulation Major, Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151 921, Korea
    Theriogenology 72:519-27. 2009
  101. pmc Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos
    Guy S Eakin
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Avenue, New York, New York 10021, USA
    Nat Protoc 1:1145-53. 2006
    ..This increasingly popular form of chimera produces embryos of nearly complete ES cell derivation that can be used to speed transgenic production or ask ..

Research Grants73

  1. Development of Vaccines against Clostridium difficile Infection
    SAUL R TZIPORI; Fiscal Year: 2013
    ..difficile toxin A and B generated by point mutations (designated as aTxAB), and a chimera protein containing full-length TcdB but with its receptor binding domain replaced with that of TcdA (designated ..
  2. Platelet TSP1 Mediates Vascular Disease and pulmonary hypertension in Sickle Cell
    Enrico M Novelli; Fiscal Year: 2013
    ..Extensive preliminary data using chimera cross transplantation systems confirm that CD47 is critical in the development of PH in the transgenic sickle ..
  3. Role of Sex Chromosome Complement in the CNS during Autoimmune Disease
    Sienmi Du; Fiscal Year: 2013
    ..Irradiation bone marrow chimera is a widely used tool in basic immunology to manipulate the immune system independently from other tissues...
  4. The relative roled of sex and genes during development of systemic autoimmunity
    ..We will do so by investigating disease development patterns in fetal liver chimera mice, including analyses of the immune system phenotype and end-organ, i.e. kidney, involvement (aim 1...
  5. Trophokine for regenerative medicine
    Jianmin Fang; Fiscal Year: 2010
    ..To test this possibility, Michieli et al., (2002) created a recombinant single-chain chimera consisting of a truncated HGF a-chain and a truncated MSP (macrophage-stimulating protein, a cytokine with high ..
  6. Identification of Inhibitors that Stabilize ER Degradation Substrates
    Domenico Tortorella; Fiscal Year: 2012
    ..class I heavy chains in US2 and US11 cells was adapted into a high-throughput screen using a class I heavy chain chimera consisting of enhanced green fluorescence protein and class I heavy chain...
  7. Neurochemistry/physiology of proopiomelanocortin neurons
    MALCOLM JAMES LOW; Fiscal Year: 2013
    ..The techniques involved are aggregation chimera formation and Cre recombinase-mediated reactivation of POMC expression from a neuron-specific and reversibly ..
  8. Inhibition of CCR1/CCR5 mediated angiogenesis and joint destruction by EGCG
    Salah Uddin Ahmed; Fiscal Year: 2010
    ..rat adjuvant-induced arthritis (AIA) model and in RA synovial tissue (ST)-severe combined immunodeficient (SCID) chimera by blocking CCR1/CCR5 receptor expression...
  9. Immune response to Pneumocystis and bone marrow failure
    Nicole Meissner; Fiscal Year: 2013
    ..Bone marrow chimera experiments between IFrag- /- and RAG-/- mice revealed that IFNAR expression is required on hematopoietic cells ..
  10. A search for genes that regulate allogeneic stem cell competition
    Irving L Weissman; Fiscal Year: 2013
    ..Botryllus colonies that share one allele form chimeras, colonies that do not, reject. Botryllus chimera formation utilizes a system analogous to NK cells...
  11. Isoform specific effects of the autoinhibitory element and the C-terminus of nitr
    JOHN SALERNO; Fiscal Year: 2009
    ..Related chimera have been produced in a number of laboratories, establishing the feasibility of the approach...
  12. NO-Zinc Signaling in Intact Pulmonary Endothelium
    CLAUDETTE MARIE ST CROIX; Fiscal Year: 2010
    ..contemporary optical microscopy and fluorescent reporter molecules, including a green fluorescent protein (GFP) chimera of the zinc-binding protein metallothionein (MT) suitable for fluorescence resonance energy transfer (FRET) ..
  13. Induced Pluripotent Stem Cells from Swine: application to genetic modification
    ROBERT MICHAEL ROBERTS; Fiscal Year: 2013 vitro and in vivo (teratoma) criteria for pluripotency will then be tested for their ability to participate in chimera production...
  14. Basis for Lymphomagenesis in Akt2 Transgenic Mice
    Joseph R Testa; Fiscal Year: 2013
    ..In addition, bone marrow chimera experiments will be carried out to determine if knock down of Dlx5 in non-malignant marrow cells from a founder ..
  15. High Throughput Transcriptome Sequencing for Systematic Detection of Recurrent Tr
    Chandan Kumar; Fiscal Year: 2012 multiple novel gene fusions and RNA chimeras in prostate cancer, including the discovery of a recurrent chimera, SLC45A3-ELK4 in a subset of prostate cancer tissues (11)...
  16. Optimized Adaptation of Simian-tropic R5 HIV Clade C to Pig-tailed Macaques
    RUTH MARGRIT RUPRECHT; Fiscal Year: 2013
    ..The chimera is replication-competent in peripheral blood mononuclear cells (PBMC) of M. nemestrina...
    John W Christman; Fiscal Year: 2013
    ..Using reciprocal bone marrow chimera p47phox-/- wt mice, we observed that p47phox-/- bone marrow in wt mice resulted in enhanced NF-[unreadable]B ..
  18. Novel lysine deacetylase 6 Hsp domain inhibitors against AML
    CHUNG JEN JAMES CHOU; Fiscal Year: 2013
    ..kinase mutations in FLT-3, c-Kit, and Ras and constitutively active transcription factors such as STAT5 and chimera MLL relevant to disease outcome...
  19. A novel vaccine to prevent HCV infection and hence liver cancer
    Ellen S Vitetta; Fiscal Year: 2010
    ..These peptoids will be screened with these antibodies, and those that react will be coupled to carrier proteins and used to immunize mice. The mice should make antibodies that then both recognize both the peptoid and the virus. ..
  20. Understanding of a Family of Bacterial Transcription Factors
    Hwan Youn; Fiscal Year: 2012
    ..binding and the other for DNA binding) is well characterized, the approach enables us to either activate the chimera (via cAMP, the ligand of CRP) or to functionally assay the protein (via the known target DNA of CRP)...
  21. Real-time Measurement of Self-antigen Recognition by Lymphocytes In Vivo
    ALEX YEE CHEN HUANG; Fiscal Year: 2012
    ..available in our own laboratory, combined with classical immunological techniques and mouse bone marrow chimera manipulations...
  22. Synergistic actions by multiple Toll-like receptors in alcoholic liver disease
    Ekihiro Seki; Fiscal Year: 2013
    ..are: Aim #1: To determine the role of TLR4 on the activation of Kupffer cells and HSCs in ASH;TLR4-bone marrow chimera will be generated and treated with intragastric ethanol feeding...
    Robert L Hendricks; Fiscal Year: 2013
    ..In Specific Aim 2 we will generate bone marrow chimera mice containing wild type CD8+ T cells and those deficient in receptors for cytokines responsible for homeostatic ..
  24. Defining the Role of MafA in Islet Beta Cells
    Roland W Stein; Fiscal Year: 2013
    ..hypothesis will be tested by analyzing the functional characteristics of transgenes expressing either a MafB/A chimera or MafB on islet [unreadable] cell function in MafA(panc mice, which lack MafA expression throughout the pancreas ..
  25. Norovirus P Particle, A Multifunctional Platform For Vaccine Development
    Ming Tan; Fiscal Year: 2012
    ..2) addition of immunogenic stimulus molecules to the antigens, and 3) addition of a purification tag to the chimera. This study will gather further evidence on the usefulness of the P particle platform for antigen presentation...
  26. TLR4 Signaling in alcoholic liver disease
    Gyongyi Szabo; Fiscal Year: 2013
    ..signals between hepatocytes and bone marrow-derived inflammatory cells in alcoholic liver injury in bone marrow chimera mice including wild-type mice with TLR4-,TRIF- or IRF3-deficient bone marrow and in TLR4-,TRIF- or IRF3- ..
    CRAIG STUART PIKAARD; Fiscal Year: 2012
    ..These efforts will contribute to our long-term goals of understanding the mechanisms responsible for nucleolar dominance and the mechanisms capable of silencing chromosomal loci on a megabase scale. ..
  28. Exploring how the genome folds through proximity ligation and sequencing
    Erez Lieberman-Aiden; Fiscal Year: 2011
    ..Observation of a chimera composed of X and Y suggests that X and Y must have been near one another in the original sample...
  29. Mechanisms of Action of UmuD in Regulating DNA Damage-Induced Genes in a umuC Def
    Janelle M Hare; Fiscal Year: 2013
    ..The genotypic and phenotypic characteristics of UmuDAb constitute a chimera of LexA and UmuD: it has an extended N-terminal domain (NTD) like LexA, and a C-terminal domain (CTD) conducting ..
  30. Novel Topoisomerase I Inhibitors
    MARK S CUSHMAN; Fiscal Year: 2013
  31. CD24 Polymorphism and Acetaminophen Toxicity
    Pan Zheng; Fiscal Year: 2010
    ..especially dendritic cells, either using a lineage-specific deletion of the CD24 gene, generation of bone marrow chimera or transgenic mice expressing CD24 exclusively on CD11c+ cells...
  32. DNA-based MR Probes for Imaging mRNA Transcripts in vivo
    Philip K Liu; Fiscal Year: 2013
    ..We will collect brain samples, quantify the PCR results, and establish the correlation between MRI DR2* and mRNA copy numbers, using disease model systems. ..
  33. Real-time imaging of tPA fluorescent hybrids in hippocampal neurons
    Janis E Lochner; Fiscal Year: 2011
    ..tPA-mediated extracellular proteolysis is also implicated in excitotoxic cell death and the neuronal degeneration associated with Alzheimer<s disease. ..
  34. Identification of Antigens for Anti-HIV Broadly Neutralizing Responses
    Steven L Zeichner; Fiscal Year: 2013
    ..However, current technology does not enable us to specifically design an MPER- scaffolding protein chimera that can drive MPER into the required shape...
  35. Nrf2 and Radiation-induced pulmonary fibrosis.
    Michael L Freeman; Fiscal Year: 2013
    ..We will use Cre-lox technology/cell fate reporter mice and bone marrow chimera experimentation to determine the contribution of resident fibroblasts, AET II cells, and bone marrow- derived ..
  36. Comprehensive identification of parent of origin effects in human and mouse
    ANDREW JAMES SHARP; Fiscal Year: 2013
    ..These studies will produce a comprehensive map of parent of origin effects in the mammalian genome, providing a strong basis for future studies of the effects of imprinting in many human diseases. ..
  37. Modifying The Risk For Developing Triple Negative Breast Cancer (TNBC)
    ..Barcellos-Hoff, my mentor for this award. This radiation chimera model in which an irradiated host is transplanted with oncogenic Trp53 null mammary epithelium exhibits an ..
  38. The role of CCR5 in HIV-1 Tat and morphine-mediated neurodegeneration.
    Elizabeth M Podhaizer; Fiscal Year: 2013
    ..A chimera mouse is being developed through breeding of Tat-transgenic mice with CCR5 null mice to examine the effects of ..
  39. Sniffer Biosensors Based on Ion Channel-GPCR Chimeras and Polylipid Membranes
    STEVEN SCOTT SAAVEDRA; Fiscal Year: 2013
    ..We propose to develop a novel class of biochemical "sniffer" sensors in which a recombinant protein chimera, comprised of an ion channel conjugated to a G-protein coupled receptor (referred to as an ICCR), is ..
  40. Translational Systems Genetics of Mitochondria, Metabolism, and Aging
    Robert W Williams; Fiscal Year: 2013
  41. Biological roles of galectins
    GERARDO RAUL VASTA; Fiscal Year: 2013
    ..Several galectin types have been described (proto, chimera, and tandem-repeat), each endowed with a unique molecular structure, biochemical properties, and taxonomic ..
  42. Genetic Analysis of Host Specificity in Schistosoma mansoni
    Tim J Anderson; Fiscal Year: 2013
    John T Lis; Fiscal Year: 2013
    ..Finally, because Aims 1-3 focus on the efficient and economical Drosophila systems, Aim 4 tests key hypotheses, which were developed and tested in Drosophila, in human tier1 ENCODE lines and mouse embryonic stem cells. ..
  44. Advanced Naturally Designed Channelrhodopsins for Photocontrol of Neural Activity
    JOHN LEE SPUDICH; Fiscal Year: 2010
    ..If needed, site-specific mutagenesis and chimera construction guided by our years of experience in microbial rhodopsin structure-function, photochemistry, and ..
  45. Intracavitary Hemostatic Agent for Non-compressible Hemorrhage
    Martin Bluth; Fiscal Year: 2007
    ..Sucrose and Fibrinogen, with a water-soluble foam inducer; the addition of thrombin and P-selectin inmunoglobin chimera, and on its form of application...
  46. Renal Protection Through PD-1/PD-L1 Interactions
    Gilbert R Kinsey; Fiscal Year: 2013 examined first in vitro by exposing isolated Tregs to plate-bound PD-L1 Fc chimeric molecules (or a control Fc chimera) and measuring the response of Tregs to PD-1 engagement at the mRNA and protein level...
  47. Transcriptional Regulation of Development and Maintenance of Memory T Lymphocytes
    H Daniel Lacorazza; Fiscal Year: 2011
    ..proliferation leading to memory T cells using immunization with dendritic cells and develop an RNAi-based chimera model to conditionally regulate endogenous Elf4 expression at will during the different phases of T cell response ..
  48. Interplay of Innate and Adaptive Immunity in Lupus Pathogenesis
    Michelle L Hermiston; Fiscal Year: 2013
    ..We will use genetic and mixed bone-marrow chimera approaches to define the impact of TLR9 on autoantibody production and identify the cellular compartments in ..
  49. Rapid and inexpensive screening of disease candidate genes in mice
    KATHLEEN JOYCE MILLEN; Fiscal Year: 2010
    ..This proposal combines recent parallel advances in RNAi technology, mouse chimera generation and focused mouse phenotyping into a novel strategy allowing inexpensive and rapid generation of ..
  50. Molecular genetics of Drosophila hybrid lethality
    Daniel A Barbash; Fiscal Year: 2012
    ..Understanding the consequences of the evolutionary patterns of these genes will help to reveal how species including humans maintain a functionally coherent genome. ..
  51. HIV vaccine development using humanized mice
    ROBERT THOMAS WOODLAND; Fiscal Year: 2013
    ..These experiments will be done with human lymphocytes using a huPBL chimera model with markedly improved B cell survival and function recently developed in our laboratory...
  52. Scalable Production of New Generation Adeno-Associated Virus Gene Therapy Vectors
    MARTIN W LOCK; Fiscal Year: 2010
    ..The overall goal of this project is to develop a large-scale production system for the commercial manufacture of a novel therapeutic for familial hypercholesterolemia. ..
  53. NanoART Manufacture, Delivery and Pharmacokinetics for Optimizing Drug Adherence
    Howard E Gendelman; Fiscal Year: 2013
    ..Third, animal studies will be performed in virus-infected rhesus macaques infected with recombinant lentivirus hybrids of SIV and HIV (SHIV) for safety and efficacy investigations, respectively. ..
  54. Mechanisms of Immune Memory Regulation by PD-1
    Surojit Sarkar; Fiscal Year: 2013
    ..In a mixed chimera setting where wild-type (WT) and PD-1 knockout (KO) CD8 T cells were present in the same WT mouse, we found that ..
  55. Molecular Regulation of Fusion: Voltage Dependence and Local Physical Interaction
    Fredric S Cohen; Fiscal Year: 2013
    ..The universality of voltage dependence of class II and III fusion proteins is now reasonably certain. Chimera experiments strongly suggest the transmembrane domain (TMD) as the region of the fusion protein that confers ..
  56. Triple action chimera: eradication of nasal S. aureus by cell wall hydrolases
    DAVID MATTHEW DONOVAN; Fiscal Year: 2010
    ..This study will explore a novel antimicrobial agent (a triple action chimeric viral enzyme) to eradicate S. aureus nasal colonization, since nasal carriage is a known risk factor for infection. ..
  57. Modified DNA Aptamers and DNAzymes for Diagnosing TB in Resource-Poor Settings
    DAN FELDHEIM; Fiscal Year: 2013
    ..Aim 3 (Months 6-12). Synthesize a chimera containing a TB DNA aptamer and Au DNAzyme and verify that each sequence functions properly. Aim 4 (Months 12-24)...
  58. Mapping and Functional Analysis of RNA:DNA Hybrid-Forming Loci
    John Greally; Fiscal Year: 2013
    ..If this exploratory project is successful, we will have a clear idea how to expand the study to a more comprehensive project in the future. PHS 398/2590 (Rev. 11/07) Continuation Format Page ..
  59. Expression and Function of the Alpha 7 Nicotinic Receptor in Schizophrenia
    Sherry Leonard; Fiscal Year: 2013
    ..Structure of CHRNA7, is complex;it is partially duplicated as a chimeric gene (CHRFAM7A). The chimera, CHRFAM7A, is expressed...
  60. Heritable variation of mRNA decay rates: An unappreciated source of gene express
    JOSHUA MICHAEL AKEY; Fiscal Year: 2013
    ..Many human diseases, such as cancer, arise from improper gene expression. This project will provide critical new insights into how genetic variation influences gene expression levels and ultimately the molecular mechanisms of disease. ..
  61. Genetic and Molecular Approach to Identify Ooplasm Reprogramming Factors
    Keith E Latham; Fiscal Year: 2010
    ..We will combine these data here to identify gene mapping, gene expression, and functional studies those specific genes that determine oocyte reprogramming capacity, and hence serve as key oocyte reprogramming factors. ..
  62. Ecological impacts of forest disturbance on La Crosse encephalitis virus dynamics
    ..We will also perform experiments to assess how efficiently mosquito species can transmit this virus. ..
    Greg Hunt; Fiscal Year: 2000
    ..3 cM with 95% confidence. It is proposed to use STS primers designed from the RAPD markers, SSCP polymorphisms and further development of another set of RAPD markers from 1000 10-mer primers, screened in bulk. ..
    Stephen Warren; Fiscal Year: 1992
  65. Clade-specific changes in transcription of olfactory genes in fruit flies
    EREZYILMAZ DENIZ; Fiscal Year: 2009
    ..This study will complement those that are restricted to the D. melanogaster model system, and will have implications for the general study of development and neuronal differentiation. ..
    MANNING CORREIA; Fiscal Year: 2007
    ..1. This channel is found in the majority of native avion vestibular hair cells. The effects of the chimera insertion will be assayed by making single channel recordings from the mutated molecules expressed in CHO cells...
  67. Structural basis of species-specific infectivities of two prion strains
    Peter M Tessier; Fiscal Year: 2010
    ..cerevisiae (Sc) and C. albicans (Ca). A robust prion species barrier exists between Sc and Ca Sup35, but a Sup35 chimera composed of domains from both species can overcome this barrier...
    John Rose; Fiscal Year: 2010
    ..These vectors could later be moved into clinical trials as HIV vaccines using antigens capable of inducing broadly reactive cellular and humoral immunity to HIV. ..
    HUNTINGTON WILLARD; Fiscal Year: 1992
    ..3. The use of these three, well-spaced genetic markers on the X will lead to the development of a one-megabase physical map for virtually the entire length of the human X chromosome...
    Ta Min Chang; Fiscal Year: 1993
    ..This information will be very useful for future formulation of more efficient therapeutic hybrid toxins...
  71. Chemoreception and Signal Amplification
    MICHAEL MANSON; Fiscal Year: 2005
    ..We have created a chimera between the NarX sensor kinase and Tar that is a repellent receptor for nitrate/nitrite...
    Robert Church; Fiscal Year: 1990
    Patrick Brown; Fiscal Year: 1999
    ..2. Further developing the enzymatic and hybridization steps in the procedure. 3. Developing PCR- based variations on GMS for affected relative pair and linkage disequilibrium mapping in the human genome. ..