yeasts

Summary

Summary: A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.

Top Publications

  1. pmc Alkali metal cation transport and homeostasis in yeasts
    Joaquin Arino
    Departament de Bioquimica i Biologia Molecular, Ed V, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    Microbiol Mol Biol Rev 74:95-120. 2010
  2. pmc Quantitative analysis of fitness and genetic interactions in yeast on a genome scale
    Anastasia Baryshnikova
    Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada
    Nat Methods 7:1017-24. 2010
  3. ncbi Yeast evolutionary genomics
    Bernard Dujon
    Institut Pasteur, Unité de Génétique Moléculaire des Levures, Centre National de la Recherche Scientifique University Pierre and Marie Curie Paris, 25 rue du Docteur Roux, F75724 Paris Cedex 15, France
    Nat Rev Genet 11:512-24. 2010
  4. ncbi Flocculation, adhesion and biofilm formation in yeasts
    Kevin J Verstrepen
    Harvard University Bauer Center for Genomics Research, 7 Divinity Avenue, Room 104, Cambridge, MA 02138, USA
    Mol Microbiol 60:5-15. 2006
  5. pmc Hunger artists: yeast adapted to carbon limitation show trade-offs under carbon sufficiency
    Jared W Wenger
    Department of Genetics, Stanford University, Stanford, California, USA
    PLoS Genet 7:e1002202. 2011
  6. pmc Osmotic stress signaling and osmoadaptation in yeasts
    Stefan Hohmann
    Department of Cell and Molecular Biology Microbiology, Goteborg University, S 405 30 Goteborg, Sweden
    Microbiol Mol Biol Rev 66:300-72. 2002
  7. ncbi Role of yeasts in table olive production
    F N Arroyo-López
    Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Edifici d Instituts, Parc Científic de Paterna, P O Box 22085, E 46071 Valencia, Spain
    Int J Food Microbiol 128:189-96. 2008
  8. pmc The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species
    Kevin P Byrne
    Department of Genetics, Smurfit Institute, University of Dublin, Trinity College, Dublin 2, Ireland
    Genome Res 15:1456-61. 2005
  9. ncbi Engineering yeasts for xylose metabolism
    Thomas W Jeffries
    USDA, Forest Service and University of Wisconsin Madison, Forest Products Laboratory, 53726, USA
    Curr Opin Biotechnol 17:320-6. 2006
  10. ncbi Intracellular signaling from the endoplasmic reticulum to the nucleus: the unfolded protein response in yeast and mammals
    C Patil
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143, USA
    Curr Opin Cell Biol 13:349-55. 2001

Detail Information

Publications314 found, 100 shown here

  1. pmc Alkali metal cation transport and homeostasis in yeasts
    Joaquin Arino
    Departament de Bioquimica i Biologia Molecular, Ed V, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    Microbiol Mol Biol Rev 74:95-120. 2010
    ..b>Yeasts have developed a number of strategies to adapt to large variations in the concentrations of these cations in the ..
  2. pmc Quantitative analysis of fitness and genetic interactions in yeast on a genome scale
    Anastasia Baryshnikova
    Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada
    Nat Methods 7:1017-24. 2010
    ....
  3. ncbi Yeast evolutionary genomics
    Bernard Dujon
    Institut Pasteur, Unité de Génétique Moléculaire des Levures, Centre National de la Recherche Scientifique University Pierre and Marie Curie Paris, 25 rue du Docteur Roux, F75724 Paris Cedex 15, France
    Nat Rev Genet 11:512-24. 2010
    ..b>Yeasts offer us a unique opportunity to examine how molecular and reproductive mechanisms combine to affect genome ..
  4. ncbi Flocculation, adhesion and biofilm formation in yeasts
    Kevin J Verstrepen
    Harvard University Bauer Center for Genomics Research, 7 Divinity Avenue, Room 104, Cambridge, MA 02138, USA
    Mol Microbiol 60:5-15. 2006
    ..These adhesion properties are of medical and industrial relevance. Pathogenic yeasts such as Candida albicans and Candida glabrata adhere to medical devices and form drug-resistant biofilms...
  5. pmc Hunger artists: yeast adapted to carbon limitation show trade-offs under carbon sufficiency
    Jared W Wenger
    Department of Genetics, Stanford University, Stanford, California, USA
    PLoS Genet 7:e1002202. 2011
    ..We conclude that yeasts that evolve high fitness under one resource-limiting condition also become more fit under other resource-limiting ..
  6. pmc Osmotic stress signaling and osmoadaptation in yeasts
    Stefan Hohmann
    Department of Cell and Molecular Biology Microbiology, Goteborg University, S 405 30 Goteborg, Sweden
    Microbiol Mol Biol Rev 66:300-72. 2002
    ....
  7. ncbi Role of yeasts in table olive production
    F N Arroyo-López
    Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Edifici d Instituts, Parc Científic de Paterna, P O Box 22085, E 46071 Valencia, Spain
    Int J Food Microbiol 128:189-96. 2008
    ..b>Yeasts can play a double role in this food...
  8. pmc The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species
    Kevin P Byrne
    Department of Genetics, Smurfit Institute, University of Dublin, Trinity College, Dublin 2, Ireland
    Genome Res 15:1456-61. 2005
    ..gen.tcd.ie/ygob) to facilitate visual comparisons and computational analysis of synteny relationships in yeasts. The data presented in YGOB, currently covering seven species, are based on sets of homologous genes that have ..
  9. ncbi Engineering yeasts for xylose metabolism
    Thomas W Jeffries
    USDA, Forest Service and University of Wisconsin Madison, Forest Products Laboratory, 53726, USA
    Curr Opin Biotechnol 17:320-6. 2006
    ..One such technology under development is the use of yeasts for the commercial fermentation of xylose to ethanol...
  10. ncbi Intracellular signaling from the endoplasmic reticulum to the nucleus: the unfolded protein response in yeast and mammals
    C Patil
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143, USA
    Curr Opin Cell Biol 13:349-55. 2001
    ..Recent results reveal that the output of the mammalian UPR reaches beyond transcriptional regulation of secretory pathway components to control of general translation, the cell cycle and programmed cell death...
  11. ncbi Genome evolution in yeasts
    Bernard Dujon
    Unité de Génétique Moléculaire des Levures, URA 2171 CNRS and UFR 927 Université Pierre et Marie Curie
    Nature 430:35-44. 2004
    ..The hemiascomycete yeasts, with their compact genomes, similar lifestyle and distinct sexual and physiological properties, provide a unique ..
  12. ncbi Yeast interactions and wine flavour
    Graham H Fleet
    Food Science and Technology, School of Chemical Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Int J Food Microbiol 86:11-22. 2003
    Wine is the product of complex interactions between fungi, yeasts and bacteria that commence in the vineyard and continue throughout the fermentation process until packaging...
  13. ncbi Interaction of pathogenic yeasts with phagocytes: survival, persistence and escape
    Katja Seider
    Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology Hans Knoell Institute Jena HKI, Jena, Germany
    Curr Opin Microbiol 13:392-400. 2010
    Pathogenic yeasts, either from the environment or the normal flora, have to face phagocytic cells that constitute the first line of defence during infection...
  14. ncbi Physiological roles of trehalose in bacteria and yeasts: a comparative analysis
    J C Arguelles
    Area de Microbiologia, Facultad de Biologia, Universidad de Murcia, Spain
    Arch Microbiol 174:217-24. 2000
    ..Recent findings also point to important biotechnological applications for trehalose...
  15. ncbi Mitochondria-dependent apoptosis in yeast
    C Pereira
    Departamento de Biologia Centro de Biologia, Universidade do Minho, Campus de Gualtar, Braga, Portugal
    Biochim Biophys Acta 1783:1286-302. 2008
    ..In conclusion, yeast with its distinctive ability to survive without respiration-competent mitochondria is a powerful model to study the involvement of mitochondria and mitochondria interacting proteins in cell death...
  16. ncbi Biogeography of the yeasts of ephemeral flowers and their insects
    M A Lachance
    Department of Plant Sciences, University of Western Ontario, London, Canada
    FEMS Yeast Res 1:1-8. 2001
    ..The communities consist mostly of yeasts in four clades centered around the genera Metschnikowia, Kodamaea, Wickerhamiella, and Starmerella...
  17. ncbi Yeasts isolated from three varieties of grapes cultivated in different locations of the Dolenjska vine-growing region, Slovenia
    Peter Raspor
    University of Ljubljana, Biotechnical Faculty, Food Science and Technology Department, Jamnikarjeva 101, 1000 Ljubljana, Slovenia
    Int J Food Microbiol 109:97-102. 2006
    The number and diversity of yeasts on grape berry surfaces are influenced by several factors, such as grape variety, degree of grape maturity at harvest, climatological conditions, geographic location, physical damage of grapes, the ..
  18. ncbi Oily yeasts as oleaginous cell factories
    Jose Manuel Ageitos
    Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Santiago de Compostela, Spain
    Appl Microbiol Biotechnol 90:1219-27. 2011
    Oily yeasts have been described to be able to accumulate lipids up to 20% of their cellular dry weight...
  19. pmc Rapid expansion and functional divergence of subtelomeric gene families in yeasts
    Chris A Brown
    Faculty of Arts and Sciences Center for Systems Biology, Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA
    Curr Biol 20:895-903. 2010
    ..However, most genome sequences do not contain assembled subtelomeric sequences, and, as a result, subtelomeres are often overlooked in comparative genomics...
  20. pmc Chemical genetic screen identifies lithocholic acid as an anti-aging compound that extends yeast chronological life span in a TOR-independent manner, by modulating housekeeping longevity assurance processes
    Alexander A Goldberg
    Department of Biology, Concordia University, Montreal, Quebec, Canada
    Aging (Albany NY) 2:393-414. 2010
    ....
  21. pmc Moonlighting proteins in yeasts
    Carlos Gancedo
    Department of Metabolism and Cell Signaling, Instituto de Investigaciones Biomedicas Alberto Sols, CSIC UAM, 28029 Madrid, Spain
    Microbiol Mol Biol Rev 72:197-210, table of contents. 2008
    ..Moonlighting activities in yeasts include such diverse functions as control of gene expression, organelle assembly, and modification of the activity ..
  22. ncbi Evidence for dynamically organized modularity in the yeast protein-protein interaction network
    Jing Dong J Han
    Center for Cancer Systems Biology and Department of Cancer Biology, Dana Farber Cancer Institute, and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 430:88-93. 2004
    ....
  23. pmc Evolutionarily conserved elements in vertebrate, insect, worm, and yeast genomes
    Adam Siepel
    Center for Biomolecular Science and Engineering, University of California, Santa Cruz, Santa Cruz, California 95064, USA
    Genome Res 15:1034-50. 2005
    ..From yeasts to vertebrates, in order of increasing genome size and general biological complexity, increasing fractions of ..
  24. ncbi Ammonia mediates communication between yeast colonies
    Z Palkova
    Department of Genetics and Microbiology, Charles University, Prague, Czech Republic
    Nature 390:532-6. 1997
    ..Although non-motile, yeasts can create organized structures, colonies in which cells communicate and act in a coordinated fashion...
  25. pmc Starvation induced cell death in autophagy-defective yeast mutants is caused by mitochondria dysfunction
    Sho W Suzuki
    Frontier Research Center, Tokyo Institute of Technology, Yokohama, Japan
    PLoS ONE 6:e17412. 2011
    ..The failure of response to starvation is the major cause of cell death in atg mutants...
  26. pmc Yeast genome-wide screen reveals dissimilar sets of host genes affecting replication of RNA viruses
    Tadas Panavas
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Proc Natl Acad Sci U S A 102:7326-31. 2005
    ..This finding suggests that virus infection and disease-causing proteins might use or alter similar host pathways and may suggest connections between chronic diseases and prior virus infection...
  27. ncbi Metabolic engineering for improved fermentation of pentoses by yeasts
    T W Jeffries
    USDA Forest Service, Forest Products Laboratory, Madison, WI 53726 2398, USA
    Appl Microbiol Biotechnol 63:495-509. 2004
    ..Respiration is critical for growth on xylose by both native xylose-fermenting yeasts and recombinant S, cerevisiae. Anaerobic growth by recombinant mutants has been reported...
  28. ncbi Function of yeast species and strains in wine flavour
    P Romano
    Dipartimento di Biologia, Difesa e Biotecnologie Agro Forestali, Universita degli Studi della Basilicata, Campus di Macchia Romana, Potenza 85100, Italy
    Int J Food Microbiol 86:169-80. 2003
    ..Here we report the characterization of a large number of strains of different wine yeast species, isolated from spontaneous wine fermentations and included in the culture collection of the Basilicata University...
  29. ncbi Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid
    Xiaochen Yu
    Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164 6120, USA
    Bioresour Technol 102:6134-40. 2011
    ..0% and 62.0% at 1g/L, respectively. Our work demonstrates that lipid production is a promising alternative to utilize hemicellulosic sugars obtained during pretreatment of lignocellulosic materials...
  30. pmc tRNA 5'-end repair activities of tRNAHis guanylyltransferase (Thg1)-like proteins from Bacteria and Archaea
    Bhalchandra S Rao
    Department of Biochemistry and Center for RNA Biology and Molecular, Cellular and Developmental Biology Graduate Program, The Ohio State University, Columbus, OH 43210, USA
    Nucleic Acids Res 39:1833-42. 2011
    ..These data support more widespread roles for 3'-5' nucleotide addition reactions in biology than previously expected...
  31. ncbi Unraveling moonlighting functions with yeasts
    Carmen Lisset Flores
    Department of Metabolism and Cell Signaling, Instituto de Investigaciones Biomedicas Alberto Sols, CSIC UAM, Madrid, Spain
    IUBMB Life 63:457-62. 2011
    This review considers the use of yeasts to study protein moonlighting functions. The cases discussed highlight the possibilities offered by the well-developed yeast genetics for the study of moonlighting mechanisms...
  32. ncbi Exit from the trans-Golgi network: from molecules to mechanisms
    Mihaela Anitei
    Biotechnology Center, Dresden University of Technology, Tatzberg 47 49, 01307 Dresden, Germany
    Curr Opin Cell Biol 23:443-51. 2011
    ....
  33. ncbi Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone
    M Rape
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82152, Martinsried, Germany
    Cell 107:667-77. 2001
    ..Remarkably, this enzyme binds preferentially ubiquitinated substrates, suggesting that CDC48(UFD1/NPL4) is qualified to selectively remove ubiquitin conjugates from protein complexes...
  34. pmc Real-time quantitative PCR (QPCR) and reverse transcription-QPCR for detection and enumeration of total yeasts in wine
    Núria Hierro
    Departament de Bioquimica i Biotecnologia, Facultat d Enologia, Universitat Rovira i Virgili, Marcelli Domingo s n, 43007 Tarragona, Spain
    Appl Environ Microbiol 72:7148-55. 2006
    Real-time PCR, or quantitative PCR (QPCR), has been developed to rapidly detect and quantify the total number of yeasts in wine without culturing. Universal yeast primers were designed from the variable D1/D2 domains of the 26S rRNA gene...
  35. pmc Systematic discovery of unannotated genes in 11 yeast species using a database of orthologous genomic segments
    Seán S Ohéigeartaigh
    Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland
    BMC Genomics 12:377. 2011
    ..This principle has often been used in investigations of particular genes or genomic regions, but to our knowledge it has never been implemented systematically...
  36. ncbi Yeasts in table olive processing: desirable or spoilage microorganisms?
    F N Arroyo-López
    Food Biotechnology Department, Instituto de la Grasa CSIC, Avda Padre Garcia Tejero 4, 41012 Seville, Spain
    Int J Food Microbiol 160:42-9. 2012
    b>Yeasts are unicellular eukaryotic microorganisms isolated from many foods, and are commonly found in table olive processing where they can play a double role...
  37. ncbi Controlled mixed culture fermentation: a new perspective on the use of non-Saccharomyces yeasts in winemaking
    Maurizio Ciani
    Dipartimento S A I F E T, sez di Microbiologia Alimentare, Industriale e Ambientale, Universita Politecnica delle Marche, Ancona, Italy
    FEMS Yeast Res 10:123-33. 2010
    Mixed fermentations using controlled inoculation of Saccharomyces cerevisiae starter cultures and non-Saccharomyces yeasts represent a feasible way towards improving the complexity and enhancing the particular and specific characteristics ..
  38. ncbi Determination of killer activity in yeasts isolated from the elaboration of seasoned green table olives
    Alejandro Hernandez
    Nutricion y Bromatologia, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Ctra de Cáceres s n 06071 Badajoz, Spain
    Int J Food Microbiol 121:178-88. 2008
    In this work 51 yeasts strains isolated from seasoned green table olives and belonging to the Candida, Debaryomyces, Kluyveromyces, Pichia, and Saccharomyces genera were characterized by their killer activity in different conditions...
  39. pmc Yeast populations residing on healthy or botrytis-infected grapes from a vineyard in Attica, Greece
    Aspasia A Nisiotou
    Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, Athens, Greece
    Appl Environ Microbiol 73:2765-8. 2007
    ..opuntiae from the closely related organism Hanseniaspora guilliermondii. Botrytis infection resulted in a larger population and greater diversity of yeasts enriched with fermentative or spoilage species.
  40. ncbi A high throughput screening assay for determination of chronological lifespan of yeast
    Ziyun Wu
    Food Science and Technology Programme, Department of Chemistry, National University of Singapore, Science Drive 3, Singapore
    Exp Gerontol 46:915-22. 2011
    ..In addition, the assay also provides quantifiable data including lag-time, growth rate, doubling time, and survival percentage...
  41. ncbi Spoilage yeasts in the wine industry
    V Loureiro
    Laboratorio de Microbiologia, Departamento de Botanica e Engenharia Biologica, Instituto Superior de Agronomia, 1349 017 Lisbon, Portugal
    Int J Food Microbiol 86:23-50. 2003
    b>Yeasts play a central role in the spoilage of foods and beverages, mainly those with high acidity and reduced water activity (a(w)). A few species are capable of spoiling foods produced according to good manufacturing practices (GMPs)...
  42. ncbi EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST)
    Maiken C Arendrup
    Unit of Mycology, Department of Microbiological Surveillance and Research, Statens Serum Institute, Copenhagen, Denmark
    Clin Microbiol Infect 18:E246-7. 2012
    ..for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for fermentative yeasts. Changes are: dimethylsulphoxide is now the recommended solvent for caspofungin, micafungin and fluconazole; the ..
  43. ncbi Influence of the farming system and vine variety on yeast communities associated with grape berries
    Gustavo Cordero-Bueso
    Departamento de Agroalimentacion, Instituto Madrileño de Investigación y Desarrollo Rural Agrario y Alimentario, Autovía A2, Km 38, 2 28800 Alcalá de Henares, Madrid, Spain
    Int J Food Microbiol 145:132-9. 2011
    ..The vineyard could be an important source of native yeasts of oenological interest...
  44. ncbi The Warburg and Crabtree effects: On the origin of cancer cell energy metabolism and of yeast glucose repression
    Rodrigo Diaz-Ruiz
    Oxygen Sensing and Cancer Laboratory, Ludwig Institute for Cancer Research Ltd, Karolinska Institute, Nobels väg 3, SE 171 77 Stockholm, Sweden
    Biochim Biophys Acta 1807:568-76. 2011
    ..In this paper we will review these common metabolic properties as well as the possible origins of the Crabtree and Warburg effects...
  45. pmc Identification of mating type genes in the bipolar basidiomycetous yeast Rhodosporidium toruloides: first insight into the MAT locus structure of the Sporidiobolales
    Marco A Coelho
    Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829 516 Caparica, Portugal
    Eukaryot Cell 7:1053-61. 2008
    ..suggests that this structure, which is unique among fungal lipopeptide pheromones, seems to be prevalent in red yeasts. Species comparisons provided evidence for a large, multigenic MAT locus structure in the Sporidiobolales, but no ..
  46. ncbi Nuclear pore complexes in the organization of silent telomeric chromatin
    V Galy
    Laboratoire de Biologie Cellulaire du Noyau, Institut Pasteur, CNRS URA 1773, Paris, France
    Nature 403:108-12. 2000
    ..We propose that, through these interactions, nuclear-pore complexes organize a nuclear subdomain that is intimately involved in the regulation of chromatin metabolism...
  47. ncbi Global analysis of protein phosphorylation in yeast
    Jason Ptacek
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, USA
    Nature 438:679-84. 2005
    ..Because many yeast proteins and pathways are conserved, these results will provide insights into the mechanisms and roles of protein phosphorylation in many eukaryotes...
  48. ncbi Production of recombinant proteins and metabolites in yeasts: when are these systems better than bacterial production systems?
    Danilo Porro
    Department of Biotechnology and Biosciences, University of Milan Bicocca, P zza della Scienza 2, 20126 Milan, Italy
    Appl Microbiol Biotechnol 89:939-48. 2011
    ..In this review, we compare the advantages and limitations of the principal yeast and bacterial workhorse systems...
  49. ncbi 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors
    Paola Grandi
    Cellzome AG, Meyerhofstrasse 1, 69117, Heidelberg, Germany
    Mol Cell 10:105-15. 2002
    ..We conclude that the 40S synthesis machinery predominately associates with the 35S pre-rRNA factors, whereas factors required for 60S subunit synthesis largely bind later, showing an unexpected dichotomy in binding...
  50. pmc The function of communities in protein interaction networks at multiple scales
    Anna C F Lewis
    Department of Statistics, University of Oxford, Oxford, UK
    BMC Syst Biol 4:100. 2010
    ..We investigate the link between biological modules and network communities in yeast and its relationship to the scale at which we probe the network...
  51. ncbi The importance of a functional trehalose biosynthetic pathway for the life of yeasts and fungi
    Carlos Gancedo
    Albert Sols Institute of Biomedical Research, CSIC UAM, c Arturo Duperier 4, 28029 Madrid, Spain
    FEMS Yeast Res 4:351-9. 2004
    ..an intermediate of trehalose biosynthesis, is an important metabolic regulator in such different organisms as yeasts or plants...
  52. pmc Ubiquitination of tombusvirus p33 replication protein plays a role in virus replication and binding to the host Vps23p ESCRT protein
    Daniel Barajas
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Virology 397:358-68. 2010
    ..These findings argue that optimal level of p33 ubiquitination plays a regulatory role during tombusvirus infections...
  53. pmc Lipid transfer and signaling at organelle contact sites: the tip of the iceberg
    Alexandre Toulmay
    Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Cell Biol 23:458-63. 2011
    ..These findings suggest that we are just beginning to understand how MCSs form and function...
  54. pmc Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains
    S I Fujita
    Department of Laboratory Medicine, Graduate School of Medical Science, School of Medicine, Kanazawa University, 13 1 Takara machi, Kanazawa, Japan
    J Clin Microbiol 39:3617-22. 2001
    ..5 h for the PCR-AGE method and 3 h for the PCR-ME method)...
  55. pmc The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import
    B Fahrenkrog
    M E Müller Institute for Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Mol Biol Cell 11:3885-96. 2000
    ..Whereas Nup53p is directly involved in NLS-mediated protein import by its interaction with the yeast nuclear import receptor Kap95p, it appears not to participate in NES-dependent nuclear export...
  56. pmc TRAF5, a novel tumor necrosis factor receptor-associated factor family protein, mediates CD40 signaling
    T K Ishida
    Department of Oncology, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 93:9437-42. 1996
    ..These results suggest that TRAF5 and TRAF3 could be involved in both common and distinct signaling pathways emanating from CD40...
  57. pmc Evolution of transcription networks--lessons from yeasts
    Hao Li
    Department of Biochemistry and Biophysics, University of California San Francisco, CA, USA
    Curr Biol 20:R746-53. 2010
    ....
  58. ncbi In vitro and in vivo activity of tea tree oil against azole-susceptible and -resistant human pathogenic yeasts
    Francesca Mondello
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 51:1223-9. 2003
    ..Overall, the use of a reliable animal model of infection has confirmed and extended our data on the therapeutic effectiveness of TTO against fungi, in particular against C. albicans...
  59. ncbi Dynamics of protein turnover, a missing dimension in proteomics
    Julie M Pratt
    Department of Veterinary Preclinical Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZJ, United Kingom
    Mol Cell Proteomics 1:579-91. 2002
    ....
  60. ncbi ERAD: the long road to destruction
    Birgit Meusser
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13092 Berlin, Germany
    Nat Cell Biol 7:766-72. 2005
    ..In this review, we will summarize advances in various aspects of ERAD and discuss new findings on how substrate dislocation is achieved...
  61. ncbi Proteolysis and the cell cycle: with this RING I do thee destroy
    M Tyers
    Programme in Molecular Biology and Cancer, Graduate Department of Molecular and Medical Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, M5G 1X5, M5S 1A8, Canada
    Curr Opin Genet Dev 10:54-64. 2000
    ..The circuits that interconnect the SCF, APC/C and cyclin-dependent kinase activities form a master oscillator that coordinates the replication and segregation of the genome...
  62. ncbi Multiple rounds of speciation associated with reciprocal gene loss in polyploid yeasts
    Devin R Scannell
    Smurfit Institute of Genetics, University of Dublin, Trinity College, Dublin 2, Ireland
    Nature 440:341-5. 2006
    ..We propose a simple, unified model in which a single mechanism--passive gene loss-enabled whole--genome duplication and led to the rapid emergence of new yeast species...
  63. ncbi Large-scale production of functional membrane proteins
    F Junge
    Centre for Biomolecular Magnetic Resonance, University of Frankfurt Main, Institute for Biophysical Chemistry, Frankfurt Main, Germany
    Cell Mol Life Sci 65:1729-55. 2008
    ..The review will give an overview of currently established processes for the production of functionally folded membrane proteins...
  64. ncbi Domain combinations in archaeal, eubacterial and eukaryotic proteomes
    G Apic
    Laboratory of Molecular Biology, MRC, Hills Road, Cambridge, CB2 2QH, UK
    J Mol Biol 310:311-25. 2001
    ..Finally, we compare the set of the domain combinations in the genomes to those in the RCSB Protein Data Bank, and discuss the implications for structural genomics...
  65. pmc Inventory and analysis of the protein subunits of the ribonucleases P and MRP provides further evidence of homology between the yeast and human enzymes
    Magnus Alm Rosenblad
    Department of Medical Biochemistry, Institute of Biomedicine, Goteborg University, Box 413 SE 405 30 Goteborg, Sweden
    Nucleic Acids Res 34:5145-56. 2006
    ..We suggest that this motif is a binding site for the Pop3/Rpp38 proteins and we discuss other structural features of the RNA subunit and possible relationships to the protein subunit repertoire...
  66. ncbi Yeast and filamentous fungi as model organisms in microbody research
    Ida J van der Klei
    Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, PO Box 14, 9750 AA Haren, The Netherlands
    Biochim Biophys Acta 1763:1364-73. 2006
    ..This paper summarizes the major advances in microbody research using fungal systems and specifies specific properties and advantages/disadvantages of the major model organisms currently in use...
  67. ncbi Isolation of epiphytic yeasts with potential for biocontrol of Aspergillus carbonarius and A. niger on grape
    Gianluca Bleve
    Institute of Sciences of Food Production ISPA, Lecce Section, CNR, Via provinciale Lecce Monteroni, 73100 Lecce, Italy
    Int J Food Microbiol 108:204-9. 2006
    Antagonistic yeasts were isolated from the epiphytic flora associated with grape berries cv. Negroamaro and identified at species level using molecular methods...
  68. ncbi Deubiquitination of histone H2B by a yeast acetyltransferase complex regulates transcription
    Jeremy A Daniel
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Biol Chem 279:1867-71. 2004
    ..Collectively, these data suggest that the SAGA and SLIK HAT complexes can regulate an integrated set of multiple histone modifications, counteracting repressive effects that alter chromatin and regulate gene expression...
  69. ncbi Differential translation efficiency of orthologous genes is involved in phenotypic divergence of yeast species
    Orna Man
    Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Genet 39:415-21. 2007
    ..We conclude that, like factors such as transcription regulation, translation efficiency affects and is affected by the process of species divergence...
  70. ncbi SBF cell cycle regulator as a target of the yeast PKC-MAP kinase pathway
    K Madden
    Department of Biology, Yale University, Post Office Box 208103, New Haven, CT 06520 8103, USA
    Science 275:1781-4. 1997
    ..These studies provide evidence for a direct role of the PKC1 pathway in the regulation of the yeast cell cycle and cell growth and indicate that conserved signaling pathways can act to control key regulators of cell division...
  71. ncbi Characterization of the yeast ecosystem in grape must and wine using real-time PCR
    K Zott
    Universite de Bordeaux, ISVV, Bordeaux Aquitaine, INRA UMR 1219, 210 Chemin de Leysotte, CS 50008, 33882 Villenave d Ornon Cedex, France
    Food Microbiol 27:559-67. 2010
    ..primers for real-time quantitative PCR(QPCR) were designed to analyse several important non-Saccharomyces yeasts (Issatchenkia orientalis, Metschnikowia pulcherrima, Torulaspora delbrueckii, Candida zemplinina and Hanseniaspora ..
  72. ncbi Genetic aspects of targeted insertion mutagenesis in yeasts
    U Klinner
    RWTH Aachen, Institut für Biologie IV Mikrobiologie und Genetik, Worringer Weg, D 52056 Aachen, Germany
    FEMS Microbiol Rev 28:201-23. 2004
    ..Consequently, the behaviour of such non-Saccharomyces yeasts is reviewed against the background of the knowledge of targeted insertion mutagenesis in S. cerevisiae...
  73. ncbi Biosynthesis and bioproduction of coenzyme Q10 by yeasts and other organisms
    Makoto Kawamukai
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue 690 8504, Japan
    Biotechnol Appl Biochem 53:217-26. 2009
    ..The biosynthetic pathway that converts p-hydroxybenzoate into CoQ consists of eight steps in yeasts. The five enzymes involved in the biosynthetic pathway have been identified in both yeasts, yet the functions of ..
  74. ncbi Yeasts associated to Malbec grape berries from Mendoza, Argentina
    M Combina
    Estacion Experimental Agropecuaria Mendoza, Instituto Nacional de Tecnologia Agropecuaria INTA, Mendoza, Argentina
    J Appl Microbiol 98:1055-61. 2005
    ..The aims of this work were to evaluate different pre-isolation treatments applied to complete yeast extraction from grapes and to identify the yeast microflora associated to Malbec grapes from two vineyards located in Mendoza, Argentina...
  75. ncbi Design of anti-parasitic and anti-fungal hydroxy-naphthoquinones that are less susceptible to drug resistance
    Louise M Hughes
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail, Hanover, NH 03755, USA
    Mol Biochem Parasitol 177:12-9. 2011
    ..One of the new inhibitors is highly effective against an atovaquone resistant Plasmodium and illustrates the type of modification to the hydroxy-naphthoquinone ring of atovaquone that might mitigate drug resistance...
  76. pmc Aggregation of expanded polyglutamine domain in yeast leads to defects in endocytosis
    Anatoli B Meriin
    Department of Biochemistry, Boston University School of Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA
    Mol Cell Biol 23:7554-65. 2003
    ..Aggregation of polyQ in mammalian HEK293 cells also led to defects in endocytosis. Therefore, it appears that inhibition of endocytosis is a direct consequence of polyQ aggregation and could significantly contribute to cytotoxicity...
  77. ncbi Yeast dynamics during spontaneous wine fermentation of the Catalanesca grape
    Elena Di Maro
    Dipartimento di Scienza degli Alimenti, Sezione di Microbiologia Agraria, Alimentare e Ambientale e di Igiene, Stazione di Microbiologia Industriale, Universita degli Studi di Napoli Federico II, Via Universita 100, 80055 Portici, Italy
    Int J Food Microbiol 117:201-10. 2007
    ..b>Yeasts were identified using molecular tools: PCR-DGGE and partial sequence analysis of the 26S rRNA gene from isolates...
  78. ncbi Application and evaluation of denaturing gradient gel electrophoresis to analyse the yeast ecology of wine grapes
    Cheunjit J Prakitchaiwattana
    Food Science and Technology, School of Chemical Engineering and Industrial Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia
    FEMS Yeast Res 4:865-77. 2004
    The performance of denaturing gradient gel electrophoresis (DGGE) for analysing yeasts associated with wine grapes was compared with cultural isolation on malt extract agar (MEA)...
  79. ncbi An overview of lipid metabolism in yeasts and its impact on biotechnological processes
    Athanasios Beopoulos
    AgroParisTech, UMR1319, Micalis, Centre de Biotechnologie Agro Industrielle, Thiverval Grignon, France
    Appl Microbiol Biotechnol 90:1193-206. 2011
    ..This review focuses on the ongoing research on different oleaginous yeasts producing high added value lipids and on the prospects of such microbial oils to be used in different ..
  80. pmc Yeasts in floral nectar: a quantitative survey
    Carlos M Herrera
    Estacion Biologica de Donana, Consejo Superior de Investigaciones Cientificas, Sevilla, Spain
    Ann Bot 103:1415-23. 2009
    ..Possible correlations between interspecific differences in yeast incidence and pollinator composition are also explored...
  81. ncbi A history of research on yeasts 10: foundations of yeast genetics
    James A Barnett
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Yeast 24:799-845. 2007
  82. ncbi Characterization of yeasts involved in the ripening of Pecorino Crotonese cheese
    F Gardini
    Dipartimento Scienze degli Alimenti, Universita di Bologna, Piazza Goidanich, 60, 47023 Cesena, Italy
    Food Microbiol 23:641-8. 2006
    ..In addition they resulted in the highest aminobiogenic potential decarboxylating ornithine, phenylalanine, tyrosine and lysine. However, they were not able to produce histamine, biogenic amine produced by three strains of D. hansenii...
  83. ncbi Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast
    F Uhlmann
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 103:375-86. 2000
    ..Cleavage of Sccl in metaphase arrested cells is sufficient to trigger the separation of sister chromatids and their segregation to opposite cell poles...
  84. ncbi Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs
    Z Kiss-László
    Laboratorie de Biologie Moléculaire Eucaryote du CNRS, Toulouse, France
    Cell 85:1077-88. 1996
    ..The conclusion that sno-RNAs function in covalent modification of the sugar moieties of ribonucleotides demonstrates that eukaryotic small nuclear RNAs have a more versatile cellular function than earlier anticipated...
  85. pmc Autophagy: molecular machinery for self-eating
    T Yorimitsu
    Department of Molecular, Cellular and Developmental Biology, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Death Differ 12:1542-52. 2005
    ..Analyses in a range of model systems have provided huge advances toward understanding the molecular basis of autophagy. Here we review our current knowledge on the machinery and molecular mechanism of autophagy...
  86. ncbi Ecology of yeasts in plant-bumblebee mutualism in Central Europe
    Michael Brysch-Herzberg
    Department of Biology, Philipps University Marburg, 35043 Marburg, Germany
    FEMS Microbiol Ecol 50:87-100. 2004
    Yeast community involved in plant-bumblebee mutualism was investigated in three successive years. Yeasts were isolated from floral nectar, bumblebee queens after hibernation, bumblebee workers, and the honey provisions in nests...
  87. pmc Genome-wide computational prediction of tandem gene arrays: application in yeasts
    Laurence Despons
    Universite de Strasbourg, CNRS UMR7156, Strasbourg, France
    BMC Genomics 11:56. 2010
    ..The originality of this method lies in the search of protein sequence similarities in the vicinity of each coding sequence, which allows the prediction of tandem duplicated gene copies independently of their functionality...
  88. ncbi Maximizing the potential of functional genomics
    Lars M Steinmetz
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Rev Genet 5:190-201. 2004
  89. ncbi Signal pathway integration in the switch from the mitotic cell cycle to meiosis in yeast
    Saul M Honigberg
    Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri Kansas City, 5007 Rockhill Rd, Kansas City, MO 64112, USA
    J Cell Sci 116:2137-47. 2003
    ..Thus the signal network that controls the switch from growth to meiotic differentiation provides a signaling code that translates different combinations of extracellular signals into appropriate cellular responses...
  90. pmc The first N-terminal amino acids of alpha-synuclein are essential for alpha-helical structure formation in vitro and membrane binding in yeast
    Katherina Vamvaca
    Center for Neurologic Diseases, Brigham and Women s Hospital and Department of Neurology, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA
    J Mol Biol 389:413-24. 2009
    ..Variants with distinct structural preferences, identified here by a reductionist approach, provide valuable tools for elucidating the nature of toxic forms of alpha-syn in neurons...
  91. ncbi Bioproduction of resveratrol and stilbene derivatives by plant cells and microorganisms
    David Donnez
    Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vigne et Vins de Champagne Stress et Environnement, EA 2069, UFR Sciences Exactes et Naturelles, Universite de Reims Champagne Ardenne, BP 1039, 51687 Reims Cedex 02, France
    Trends Biotechnol 27:706-13. 2009
    ..This review aims at describing and comparing these different available methods, with a focus on their respective advantages, limits and perspectives as a basis for scale-up in large culture volumes...
  92. ncbi Selective degradation of peroxisomes in yeasts
    Anna Rita Bellu
    Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, 9751 NN Haren, The Netherlands
    Microsc Res Tech 61:161-70. 2003
    ..which occurs when the organelles have become redundant-have been performed, especially using methylotrophic yeasts. The finding that pexophagy and other transport pathways to the vacuole (vacuolar protein sorting, autophagy, ..
  93. ncbi Pathgroups, a dynamic data structure for genome reconstruction problems
    Chunfang Zheng
    Département d informatique et de recherche opérationnelle, Universite de Montreal, Canada
    Bioinformatics 26:1587-94. 2010
    ..Available heuristics dedicated to each of these problems are computationally costly for even small instances...
  94. ncbi A comparative study on the biosorption characteristics of some yeasts for Remazol Blue reactive dye
    Zümriye Aksu
    Department of Chemical Engineering, Hacettepe University, 06532, Beytepe, Ankara, Turkey
    Chemosphere 50:1075-83. 2003
    Biosorption capacities and rates of different kinds of dried yeasts (Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces marxianus, Candida sp., C. tropicalis, C. lipolytica, C. utilis, C. quilliermendii and C...
  95. ncbi Yeast glutamine-fructose-6-phosphate aminotransferase (Gfa1) requires methionine aminopeptidase activity for proper function
    Benjamin Dummitt
    Edward A Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 280:14356-60. 2005
    ..Additionally, Gfa1 will be a relevant target in therapeutic or physiological applications in which MetAP activity is inhibited...
  96. pmc Haploinsufficiency and the sex chromosomes from yeasts to humans
    Michaela de Clare
    Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, UK
    BMC Biol 9:15. 2011
    ..Haploinsufficiency is increasingly implicated in human disease, and so predicting this phenotype could provide insights into the genetic mechanisms behind many human diseases, including some cancers...
  97. ncbi Yeast water channels: an overview of orthodox aquaporins
    Graça Soveral
    Department Química, FCT UNL, 2829 516 Caparica, Portugal
    Biol Cell 103:35-54. 2010
    ..Although the presence of aquaporins clearly enhances membrane water permeability, the relevance of these ubiquitous water channels in yeast performance remains obscure...
  98. pmc TLC1 RNA nucleo-cytoplasmic trafficking links telomerase biogenesis to its recruitment to telomeres
    Franck Gallardo
    Departement de Biochimie, Universite de Montreal, Montreal, Quebec, Canada
    EMBO J 27:748-57. 2008
    ..Altogether, our results reveal that the nucleo-cytoplasmic trafficking of the TLC1 RNA is an important step in telomere homeostasis, and link telomerase biogenesis to its recruitment to telomeres...
  99. ncbi Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water
    P Walther
    Zentrale Einrichtung Elektronenmikroskopie, Universitaet Ulm, Albert Einstein Allee 11, 89069 Ulm, Germany
    J Microsc 208:3-10. 2002
    ..The effect was especially pronounced in yeast cells, where membrane visibility was poor after freeze-substitution with water-free medium but good after addition of 5% water to the substitution medium...
  100. ncbi Wine yeasts for the future
    Graham H Fleet
    Food Science, School of Chemical Sciences and Engineering, University of New South Wales, Sydney, NSW, Australia
    FEMS Yeast Res 8:979-95. 2008
    ..Within the total production chain, the alcoholic fermentation of grape juice by yeasts is a key process where winemakers can creatively engineer wine character and value through better yeast management ..
  101. pmc Matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates
    G Marklein
    Institute of Medical Microbiology, Immunology and Parasitology, Rheinische Friedrich Wilhelms Universitat, Bonn, Germany
    J Clin Microbiol 47:2912-7. 2009
    The clinical impact of severe infections with yeasts and yeast-like fungi has increased, especially in immunocompromised hosts...

Research Grants65

  1. Biomarkers of Fusarium biofilm formation in vitro and in vivo
    PRANAB KUMAR MUKHERJEE; Fiscal Year: 2012
    ..Fungal keratitis is predominantly caused by filamentous fungi Fusarium, but yeasts (e.g. Candida) can also cause this disease...
  2. Molecular Regulation of Alpha-Synuclein Misfolding and Toxicity in Two Yeast Mode
    SHUBHIK DEBBURMAN; Fiscal Year: 2009
    ..familial mutants (E46K/A53T, A30P/E46K, and A30P/E46K/A53T), in localization, aggregation, and toxicity in both yeasts. 2) To test the hypothesis that specific phospholipid composition and total phospholipid content is critical to a-..
  3. Genetic and Evolutionary Basis of Fungal Drug Resistance
    Zhenglong Gu; Fiscal Year: 2013
    ..why deletion of PDR5, a contributing genetic factor we have already identified, increases AmB resistance in yeasts. We hypothesize that PDR5 and its paralogous genes are involved in ergosterol homeostasis, which will be tested in ..
  4. FASEB SRC on Yeast Chromosome Structure, Replication and Segregation
    Scott Keeney; Fiscal Year: 2012
    ..Many critical questions in these areas remain unresolved, and yeasts are outstanding model organisms for addressing these questions because of the evolutionary conservation of many of ..
  5. RNA CONFORMATION AND CATALYSIS
    David P Bartel; Fiscal Year: 2013
    ..Although RNAi has been lost in Saccharomyces cerevisiae, it is present in other budding yeasts, including Saccharomyces castellii (a close relative of S...
  6. Checkpoints and double strand breaks in S. pombe meiosis
    Susan L Forsburg; Fiscal Year: 2013
    ..Studies in model organisms, especially yeasts, has provided critical insights into the mechanisms by which these kinases function in proliferating cells...
  7. HA Surface Presented Yeast Flu Vaccine and Its Enhancement by CD154 Codisplay
    Kaiming Ye; Fiscal Year: 2012
    ..protein such as avian influenza H5N1 hemagglutinin (HA) on yeast surface in a manner such that these recombinant yeasts can serve as vaccines against influenza. Yeast is known of being able to express functional HA...
  8. Investigation of the structure and function of urea amidolyase
    MARTIN RAOUL ST. MAURICE; Fiscal Year: 2012
    ..to pathogenicity by initiating a morphological switch that permits the organism to transition from unicellular yeasts t multicellular filamentous hyphae...
  9. N-acetylglucosamine signaling in fungi
    James B Konopka; Fiscal Year: 2012
    ..Therefore, C. albicans and related yeasts will be used as model systems to define novel mechanisms of GlcNAc signaling...
  10. DNA REPLICATION AND CHROMOSOME STRUCTURE IN YEAST
    Virginia A Zakian; Fiscal Year: 2013
    ..to the understanding of mechanisms responsible for the faithful replication of eukaryotic chromosomes, using yeasts as models...
  11. Control and Function of Histone H3 Lysine 27 Methylation in Neurospora
    Eric U Selker; Fiscal Year: 2013
    ..H3K27me3 has not been found in other simpler systems (e.g. yeasts). Specific aims of the project are: 1...
  12. Chemical-genetic functional annotation of the genome of a meningitis pathogen
    Hiten D Madhani; Fiscal Year: 2013
    ..In particular, as genome-wide knockout collections in model yeasts have been instrumental to the dissection of fundamental eukaryotic cellular processes, the generation and analysis ..
  13. Kinetochore-Microtubule Interactions
    JOHN RICHARD MCINTOSH; Fiscal Year: 2012
    ..accomplished on both ring-shaped microtubule- binding proteins, like the Dam1 complex (aka DASH) from budding yeasts, and fibrous complexes, like NDC80 (Hec1) from C. elegans, yeasts, and human cells...
  14. New elements of the Target of Rapamycin pathway in Candida albicans
    JULIA R KOEHLER; Fiscal Year: 2012
    ..The centrality of this pathway to many essential cellular processes has emerged from work in model yeasts and in metazoans. In C. albicans pathogenesis, Tor signaling is predicted to play a crucial role for three reasons...
  15. New insights into the role of the Fbw7 tumor suppressor in senescence and aging
    Hiroyuki Inuzuka; Fiscal Year: 2013
    ..recent studies revealed that mTOR signaling also negatively regulates longevity across species (from yeasts to mice)...
  16. GM-CSF Regulation of Zinc in Histoplasmosis
    George S Deepe; Fiscal Year: 2012
    ..Killing of yeasts by stimulated macrophages is mediated by zinc starvation...
  17. Mechanisms of the Unusual Cytokinesis in Trypanosomes
    Ziyin Li; Fiscal Year: 2013
    ..The cleavage plane in a dividing trypanosome is not defined by the central spindle like in yeasts and animals, but rather by the position of the new flagellum and the flagellum attachment zone (FAZ)...
  18. Genomic Regulation at the Nuclear Periphery
    CHARLES PATRICK LUSK; Fiscal Year: 2013
    ..While we have now deciphered the entire genome sequences of eukaryotic species from yeasts to humans, how those genomes are physically organized to support genomic functions remains an open question...
  19. Chemically-Rich Structure and Dynamics in the Active Site of Tryptophan Synthase
    Leonard J Mueller; Fiscal Year: 2013
    ..Tryptophan synthase homologues are found in bacteria, yeasts, molds, plants, and some protozoans...
  20. Metabolic Engineering for Microbial Taxol Biosynthesis
    Gregory Stephanopoulos; Fiscal Year: 2012
    ..in pathway construction, optimization, and natural product synthesis and functional expression in bacteria and yeasts of genes from plants and other sources that are critical for taxol biosynthesis, support the overall objective of ..
  21. Composition, Structure, Dynamics and Function of C-Type Lectin Receptor Domains
    Nancy L Thompson; Fiscal Year: 2013
    ..One such receptor, DC-SIGN, binds to a large variety of pathogens, including dengue virus, HIV and yeasts, by recognizing carbohydrates on the pathogen surfaces...
  22. Role of Spo11 and recombination in mouse meiosis
    Scott Keeney; Fiscal Year: 2013
    ..to formation of both crossovers and noncrossovers;much is known about the pathways leading to these products in yeasts, but comparatively little is known about these mechanisms in mammals...
  23. Investigate the multifunctional adhesins in Cryptococcus
    Xiaorong Lin; Fiscal Year: 2013
    ..Aim 2) Characterize a compendium of potential adhesins and examine their expression pattern in yeasts, hyphae and spores...
  24. GLUCOSE SENSING AND SIGNALLING IN YEAST
    H MARK JOHNSTON; Fiscal Year: 2011
    ..b>Yeasts control glucose utilization at its first, rate- limiting step - transport into the cell...
  25. Identifying Conserved Genetic Networks for Eukaryotic MMR Genes
    Winfried Edelmann; Fiscal Year: 2012
    ..divergent model eukaryotes, the fission (Schizosaccharomyces pombe;Sp) and budding (Saccharomyces cerevisiae;Sc) yeasts. We will utilize this information to predict (and test) synthetic sick or lethal interactions in defined MMR- ..
  26. A Study of DNA Replication Origins by Comparative Functional Genomics
    Ivan Liachko; Fiscal Year: 2012
    ..use comparative genomic approaches to characterize the mechanisms of ARS recognition utilized by the different yeasts. I will also assay the evolution of ARS elements and their genomic locations...
  27. nCounter Analysis System
    Nir Hacohen; Fiscal Year: 2010
    ..cell lineages, the regulatory roles of large non-coding RNAs (lincRNAs), and the evolution of gene regulation in yeasts. Preliminary data using an instrument on loan from Nanostring demonstrated that this technology generates ..
  28. FACTORS CONTROLLING TRANSCRIPTION AND CHROMATIN IN YEAST
    FRED M WINSTON; Fiscal Year: 2013
    This application proposes to study fundamental aspects of transcription and chromatin regulation, using the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe...
  29. Genome Scale Discovery of Mycobacterial Gene Function by Synthetic Genetic Arrays
    Todd Gray; Fiscal Year: 2013
    ..Hundreds of thousands of synthetic interactions have been described in yeasts and E. coli, and typically a single gene displays ~30 interacting partners...
  30. Research supplement to promote diversity/Towards a comprehensive protein interact
    Haiyuan Yu; Fiscal Year: 2013
    ..valuable information would be obtained by comparing the interactome networks from both organisms since these two yeasts diverged around 330-420 million years ago. Additionally, analysis of interaction networks in S...
  31. The Regulation of DNA Replication Kinetics
    NICHOLAS R RHIND; Fiscal Year: 2013
    ..The complimentary strengths of these evolutionarily distant yeasts allow for a more rigorous test of our model...
  32. THE TRANSITIONAL ER-GOLGI SYSTEM IN BUDDING YEASTS
    Benjamin S Glick; Fiscal Year: 2012
    ..These ideas will be explored with the aid of the budding yeasts Pichia pastoris and Saccharomyces cerevisiae. P...
  33. Asynchronous mitosis in multinucleate cells
    AMY SUSANNE GLADFELTER; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): In organisms ranging from single-celled yeasts to mammals, genetically identical cells exhibit variable cell division cycle times even when growing in the same environment...
  34. Dynamics and evolution of cell cycle transitions
    Jan M Skotheim; Fiscal Year: 2013
    ..Across yeasts, the core function of the genetic control network at the interface between the pheromone-induced MAPK pathway and ..
  35. Recombination mechanisms of crossover and noncrossover formation in Drosophila
    KIMBERLY NICOLE CROWN; Fiscal Year: 2013
    ..proposed structures of the joint molecule are primarily based on the detection of recombination intermediates in yeasts, in vitro biochemical assays, and genetic data that is difficult to interpret because of overlapping gene ..
  36. ATR-Chk1 signaling during embryonic and germ line development in C. elegans
    MATTHEW MICHAEL; Fiscal Year: 2013
    ..pathway in homogenous populations of human tissue culture cells, or simpler organisms such as budding and fission yeasts. Less well understood are the pathway's function(s) in the development and reproduction of animals, and thus ..
  37. Genetic basis of stress tolerance in natural populations of yeast
    DAVID CHARLES HESS; Fiscal Year: 2013
    ..In particular, we will observe segregation of growth ability in the face of environmental stresses that yeasts may have experienced during their natural history...
  38. The Function of Cdc14B in DNA Damage Repair and Tumorigenesis
    Pumin Zhang; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Cdc14 is a Ser/Thr phosphatase required for mitotic exit in budding yeasts. There are two homologues in mammals, Cdc14A and B. We have generated conditional Cdc14B knockout mice...
  39. MECHANISMS OF YEAST TRANSCRIPTIONAL INITIATION
    Kevin Struhl; Fiscal Year: 2002
    ..variety of experiments have pointed to common molecular mechanisms in eukaryotic organisms ranging from humans to yeasts. This proposal will continue to investigate several basic issues concerning the molecular mechanisms of ..
  40. Mechanism of Yeast Transcriptional Regulators
    Alexander D Johnson; Fiscal Year: 2013
    ..seeks to determine the detailed molecular mechanisms that underlie transcriptional rewiring using unicellular yeasts as a model system...
  41. MECHANISM OF TERMINATION OF DNA REPLICATION
    Deepak Bastia; Fiscal Year: 2010
    We propose to continue our investigations into the mechanism of action of replication terminator proteins of the yeasts S...
  42. 8th International Conference on Cryptococcus and Cryptococcosis
    Maurizio Del Poeta; Fiscal Year: 2011
    ..b>Yeasts such as Cryptococcus neoformans is responsible for more than one million annual cases of cryptococcosis worldwide ..
  43. Miniaturized DNA Sequencer for Identification of Microbial Pathogens
    Michael G Pollack; Fiscal Year: 2012
    ..demonstration 20-40 base pairs within the ITS2 genetic region were sequenced for 13 different molds and yeasts which provided unambiguous identification of each isolate...
  44. Biochemical Mechanisms and Function of DNA Helicases
    STEPHEN CHARLES KOWALCZYKOWSKI; Fiscal Year: 2013
    ..project deals with the RecQ-family of helicases, which is ubiquitous and has well-defined members in bacteria, yeasts, and humans...
  45. Mechanisms of alpha-Synulclein Inhibition of the Secretory Pathway
    Jared R Helm; Fiscal Year: 2013
    ..Recent studies in yeasts and nematodes have suggested that the primary cause of 1-synuclein toxicity is a deficit in the endoplasmic ..
  46. Epigenetic profiling by near-field UV-Raman scattering in nanochannels
    Robert Riehn; Fiscal Year: 2011
    ..chromatin or bare DNA, to construct an epigenetic map of genomic model DNA or reconstituted chromatin from yeasts. We propose a new technique for monitoring the epigenetic programming state of genomic sized molecules, ideally ..
  47. SEEKING THE YEAST SPARKING STEROL
    Martin Bard; Fiscal Year: 1991
    ..Saccharomyces and Candida to determine the sparking sterol requirements and bulk sterol requirements in these two yeasts, respectively...
  48. Biology and Drug Resistance of Candida Biofilms
    Mahmoud Ghannoum; Fiscal Year: 2005
    ..Dental plaque consists of a complex biofilm of bacteria and yeasts, predominantly Candida albicans. C...
  49. Elucidating the Biosynthesis and Transport of Heme A
    ERIC HEGG; Fiscal Year: 2006
    ..DESCRIPTION (provided by applicant): Cytochrome c oxidase is the terminal oxidase in all plants, animals, aerobic yeasts, and some bacteria...
  50. BIOSYNTHESIS OF VITAMIN B1 AND VITAMIN B6
    IAN SPENSER; Fiscal Year: 2000
    ..Vitamin B6: The origin of B6 in yeasts is unknown. It cannot be assumed a priori that the route to B6 in yeast is identical with that in E...
  51. Novel peroxisomal genes in the yeast Pichia pastoris
    James Cregg; Fiscal Year: 2005
    ..Primarily through the isolation ofpex mutants in P. pastoris and other yeasts, 25 different PEX genes have been identified and their products, (peroxins or PEX proteins) have been described...
  52. INTERACTION OF H CAPSULATUM WITH ALVEOLAR MACROPHAGE
    Simon Newman; Fiscal Year: 1999
    ..These cells initially encounter inhaled microconidia and subsequently the transformed yeasts. The sequence of events that occur from the time that conidia enter the alveolar spaces in the lung until the ..
  53. Developing Probiotics as Foods and Drugs - Scientific and Regulatory Challenges
    Patricia Hibberd; Fiscal Year: 2006
    ..Probiotics are defined as live microorganisms (such as the bacteria species Lactobacillus and Bifidobacterium, and yeasts) with beneficial effects on the host...
  54. HTS on Riboflavin and Terpene Biosynthetic Enzymes(RMI)
    Adelbert Bacher; Fiscal Year: 2005
    ..the proteins of the vitamin B2 biosynthetic pathway are known to be essential in gram negative bacteria and in yeasts. We are planning to use our expertise in the metabolic pathways mentioned above as the basis for the development ..
  55. CENTROMERE STRUCTURE AND FUNCTION
    John Carbon; Fiscal Year: 2007
    ..work to investigate centromere-kinetochores in the two most common and clinically significant pathogenic budding yeasts, Candida albicans and Candida glabrata...
  56. Role of Meiotic Genes in the Basal Eukaryote Giadia lambia
    Marianne Poxleitner; Fiscal Year: 2007
    ..Giardia is the most basal eukaryote available for study having last shared a common ancestor with man or the yeasts over 1.5 billion years ago; thus it is an ideal model organism for this project...
  57. A C. elegans localization-of-expression mapping project
    Marc Vidal; Fiscal Year: 2005
    ..One of the best illustrations of this statement is the discovery of cyclins and cyclin-dependent kinases in yeasts and sea urchins. Other examples of the role of C...
  58. A C. elegans localization-of-expression mapping project
    Marc Vidal; Fiscal Year: 2002
    ..One of the best illustrations of this statement is the discovery of cyclins and cyclin-dependent kinases in yeasts and sea urchins. Other examples of the role of C...
  59. A cholesterol biosynthetic yeast for mammalian membrane protein production
    NIALL FRASER; Fiscal Year: 2007
    ..b>Yeasts are commonly used for the heterologous production of soluble recombinant proteins...
  60. YEAST GENETIC RESEARCH RESOURCE CENTER
    Jianlong Zhou; Fiscal Year: 2004
    ..cerevisiae strains, and 400 other yeasts, representing 13 genera and 21 species...
  61. A functional genomics analysis of central carbon metabolism evolution in yeasts
    MARK STYCZYNSKI; Fiscal Year: 2009
    ..Understanding these phenomena, includingthe respiro-fermentative growth states common to some yeasts and many cancer cells, may ultimately lead to better diagnostics and therapeutics.
  62. Model Vaccines Exploiting Fungal Mannosylation
    STUART LEVITZ; Fiscal Year: 2009
    ..N-linked and O-linked glycans on yeasts are terminally mannosylated whereas fully processed mammalian glycoproteins rarely have terminal mannose groups...
  63. RNA interference in the fungal pathogen Candida albicans
    Janet Staab; Fiscal Year: 2005
    ..A salient characteristic of C. albicans is the ability to grow in different morphological forms, from ovoid yeasts to filamentous hyphae...
  64. SELECTIVE AUTOPHAGIC DEGRADATION OF PEROXISOMES IN YEAST
    James Cregg; Fiscal Year: 2004
    ..by applicant) The primary degradative compartment in the cell is the lysosome (or its equivalent in plants and yeasts, the vacuole)...
  65. INTERACTION OF H CAPSULATUM WITH MACROPHAGES AND PMN
    Simon Newman; Fiscal Year: 2000
    ..Inhaled conidia convert into the pathogenic yeast phase within an unknown time frame. Hc yeasts are phagocytized by alveolar macrophages (AM), within which they multiply...