saccharomycetales

Summary

Summary: An order of fungi in the phylum Ascomycota that multiply by budding. They include the telomorphic ascomycetous yeasts which are found in a very wide range of habitats.

Top Publications

  1. ncbi Phylogenetics of Saccharomycetales, the ascomycete yeasts
    Sung Oui Suh
    Department of Biological Sciences, Louisiana State University, Baton Rouge 70803, USA
    Mycologia 98:1006-17. 2006
  2. ncbi Selective predation, parasitism, and trophic cascades in a bluegill-Daphnia-parasite system
    Meghan A Duffy
    W K Kellogg Biological Station and Department of Zoology, Michigan State University, Hickory Corners, MI 49060, USA
    Oecologia 153:453-60. 2007
  3. pmc Initiation of meiotic chromosome synapsis at centromeres in budding yeast
    Tomomi Tsubouchi
    Howard Hughes Medical Institute, Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Genes Dev 22:3217-26. 2008
  4. pmc Comparative genomics of protoploid Saccharomycetaceae
    Jean Luc Souciet
    Universite de Strasbourg, CNRS UMR, France
    Genome Res 19:1696-709. 2009
  5. ncbi Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov
    Cletus P Kurtzman
    Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, US Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, USA
    FEMS Yeast Res 8:939-54. 2008
  6. pmc DNA relaxation dynamics as a probe for the intracellular environment
    J K Fisher
    Department of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 106:9250-5. 2009
  7. ncbi RNA regulons: coordination of post-transcriptional events
    Jack D Keene
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Box 3020, Durham, North Carolina 27710, USA
    Nat Rev Genet 8:533-43. 2007
  8. pmc Integrative analysis of cell cycle control in budding yeast
    Katherine C Chen
    Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0406, USA
    Mol Biol Cell 15:3841-62. 2004
  9. pmc Protein sequestration generates a flexible ultrasensitive response in a genetic network
    Nicolas E Buchler
    The Rockefeller University, New York, NY 10065, USA
    Mol Syst Biol 5:272. 2009
  10. pmc RNAi in budding yeast
    Ines A Drinnenberg
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 326:544-50. 2009

Detail Information

Publications254 found, 100 shown here

  1. ncbi Phylogenetics of Saccharomycetales, the ascomycete yeasts
    Sung Oui Suh
    Department of Biological Sciences, Louisiana State University, Baton Rouge 70803, USA
    Mycologia 98:1006-17. 2006
    Ascomycete yeasts (phylum Ascomycota: subphylum Saccharomycotina: class Saccharomycetes: order Saccharomycetales) comprise a monophyletic lineage with a single order of about 1000 known species...
  2. ncbi Selective predation, parasitism, and trophic cascades in a bluegill-Daphnia-parasite system
    Meghan A Duffy
    W K Kellogg Biological Station and Department of Zoology, Michigan State University, Hickory Corners, MI 49060, USA
    Oecologia 153:453-60. 2007
    ..Overall, these results suggest that selective predation does not always reduce infection prevalence, and that parasites have the potential to drastically reduce host densities even in the presence of selective predators...
  3. pmc Initiation of meiotic chromosome synapsis at centromeres in budding yeast
    Tomomi Tsubouchi
    Howard Hughes Medical Institute, Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Genes Dev 22:3217-26. 2008
    ..Our data provide evidence for two classes of synapsis initiation events that differ in location, timing, genetic requirements, and relationship to meiotic recombination...
  4. pmc Comparative genomics of protoploid Saccharomycetaceae
    Jean Luc Souciet
    Universite de Strasbourg, CNRS UMR, France
    Genome Res 19:1696-709. 2009
    ..Ancient, conserved paralogs as well as novel, lineage-specific paralogs were identified...
  5. ncbi Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov
    Cletus P Kurtzman
    Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, US Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, USA
    FEMS Yeast Res 8:939-54. 2008
    ..Lineages previously classified as varieties of Pichia kluyveri, 'Issatchenkia'scutulata, Starmera amethionina and 'Williopsis'saturnus are elevated to species rank based on sequence comparisons...
  6. pmc DNA relaxation dynamics as a probe for the intracellular environment
    J K Fisher
    Department of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 106:9250-5. 2009
    ..As a result, the technique described herein may be used as a biophysical strategy to probe the intranuclear environment...
  7. ncbi RNA regulons: coordination of post-transcriptional events
    Jack D Keene
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Box 3020, Durham, North Carolina 27710, USA
    Nat Rev Genet 8:533-43. 2007
    ..I close by considering the evolutionary wiring and rewiring of these combinatorial post-transcriptional gene-expression networks...
  8. pmc Integrative analysis of cell cycle control in budding yeast
    Katherine C Chen
    Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0406, USA
    Mol Biol Cell 15:3841-62. 2004
    ....
  9. pmc Protein sequestration generates a flexible ultrasensitive response in a genetic network
    Nicolas E Buchler
    The Rockefeller University, New York, NY 10065, USA
    Mol Syst Biol 5:272. 2009
    ..The generation of flexible ultrasensitive responses is an unappreciated adaptive advantage that could explain the frequent evolutionary emergence of dominant negatives...
  10. pmc RNAi in budding yeast
    Ines A Drinnenberg
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 326:544-50. 2009
    ..These results identify a previously unknown class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi...
  11. ncbi RAPYD--rapid annotation platform for yeast data
    Jessica Schneider
    Computational Genomics, Institute for Bioinformatics, Center for Biotechnology, Bielefeld University, Bielefeld, Germany
    J Biotechnol 155:118-26. 2011
    ..An in-depth application example of the yeast Meyerozyma guilliermondii illustrates the functionality of RAPYD. A user-friendly web interface is available at https://rapyd.cebitec.uni-bielefeld.de...
  12. ncbi Multiple levels of cyclin specificity in cell-cycle control
    Joanna Bloom
    Laboratory of Yeast Molecular Genetics, Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nat Rev Mol Cell Biol 8:149-60. 2007
    ....
  13. ncbi Metabolism of glucose and xylose as single and mixed feed in Debaryomyces nepalensis NCYC 3413: production of industrially important metabolites
    Sawan Kumar
    Applied and Industrial Microbiology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India
    Appl Microbiol Biotechnol 89:1405-15. 2011
    ..To our knowledge, this is the first study to elaborate the glucose and xylose metabolic pathway in this yeast strain...
  14. ncbi Debaryomyces hansenii--an extremophilic yeast with biotechnological potential
    Uta Breuer
    UFZ Centre of Environmental Research Leipzig Halle, Department of Environmental Microbiology, Permoserstrasse 15, D 04318 Leipzig, Germany
    Yeast 23:415-37. 2006
    ..To this end, we give a broad overview of extant biotechnological procedures using D. hansenii, e.g. in the manufacture of various foods, and propose research into the heterologous synthesis of a range of fine chemicals...
  15. ncbi Multigene phylogenetic analysis of the Trichomonascus, Wickerhamiella and Zygoascus yeast clades, and the proposal of Sugiyamaella gen. nov. and 14 new species combinations
    Cletus P Kurtzman
    Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA
    FEMS Yeast Res 7:141-51. 2007
    ..From the foregoing gene sequence analyses, the new ascosporic genus Sugiyamaella is proposed, as are 14 new species combinations and the new family Trichomonascaceae...
  16. ncbi Spathaspora arborariae sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil
    Raquel M Cadete
    Departamento de Microbiologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
    FEMS Yeast Res 9:1338-42. 2009
    ..Similar to S. passalidarum, the new species produces unconjugated asci with a single greatly elongated ascospore with curved ends. The type strain of Spathaspora arborariae sp. nov. is UFMG-HM19.1A(T) (=CBS11463(T)=NRRL Y-48658(T))...
  17. ncbi Cdc14: a highly conserved family of phosphatases with non-conserved functions?
    Annamaria Mocciaro
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Allianz, Im Neuenheimer Feld 282, 69117 Heidelberg, Germany
    J Cell Sci 123:2867-76. 2010
    ..In this Commentary, we critically review the available data on the functions of yeast and vertebrate Cdc14 phosphatases, and discuss whether they indeed share common functions as generally assumed...
  18. ncbi The dual mechanism of separase regulation by securin
    Nadine C D Hornig
    Chromosome Segregation Laboratory, WC2A 3PX, London, United Kingdom
    Curr Biol 12:973-82. 2002
    ..The molecular mechanisms underlying this dual regulation of separase by securin are unknown...
  19. pmc Testing a mathematical model of the yeast cell cycle
    Frederick R Cross
    The Rockefeller University, New York, NY 10021, USA
    Mol Biol Cell 13:52-70. 2002
    ..Thus, the model is a strong but incomplete attempt at a realistic representation of cell cycle control. Constraints of the sort developed here will be important in development of a truly predictive model...
  20. ncbi Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast
    Franz Meitinger
    German Cancer Research Centre, DKFZ ZMBH Alliance, Molecular Biology of Centrosomes and Cilia Unit, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany
    J Cell Sci 123:1851-61. 2010
    ..This defect was partially suppressed by increased levels of Cyk3. We therefore propose that the MEN directly controls cytokinesis via targeting of Inn1, Cyk3 and Chs2 to the bud neck...
  21. ncbi Cell-cycle control of gene expression in budding and fission yeast
    Jurg Bahler
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
    Annu Rev Genet 39:69-94. 2005
    ..I also compare and contrast the transcriptional regulatory networks between the two yeasts and discuss the evolutionary conservation and possible roles for cell cycle-regulated transcription...
  22. pmc Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork
    Arturo Calzada
    Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK
    Genes Dev 19:1905-19. 2005
    ..We also show that paused forks at analogous barriers in the rDNA are regulated similarly. These data indicate that paused and stalled eukaryotic replisomes resemble each other but are regulated differently...
  23. pmc Characterization of the minimum domain required for targeting budding yeast myosin II to the site of cell division
    Ida M B Lister
    Department of Cell Biology, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA
    BMC Biol 4:19. 2006
    ..Unlike most other class II myosins, the tail of Myo1 has an irregular coiled coil. In this report we use molecular genetics, biochemistry and live cell imaging to characterize the minimum localisation domain (MLD) of budding yeast Myo1...
  24. ncbi Identification and annotation of noncoding RNAs in Saccharomycotina
    José Almeida Cruz
    Architecture et Reactivite de l ARN, Institut de Biologie Moleculaire et Cellulaire du CNRS, Universite de Strasbourg, 15 rue Rene Descartes, 67084 Strasbourg Cedex, France
    C R Biol 334:671-8. 2011
    ..The results show the feasibility of automatic search for ncRNAs in full genomes and the utility of such approaches in large multi-genome sequencing and annotation projects...
  25. pmc Live-cell analysis of kinetochore-microtubule interaction in budding yeast
    Kozo Tanaka
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, UK
    Methods 51:206-13. 2010
    ..We discuss practical considerations for applying our system to live cell imaging using fluorescence microscopy...
  26. ncbi Multigene phylogenetic analysis of the Lipomycetaceae and the proposed transfer of Zygozyma species to Lipomyces and Babjevia anomala to Dipodascopsis
    Cletus P Kurtzman
    Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA
    FEMS Yeast Res 7:1027-34. 2007
    ..The proposed changes will result in the Lipomycetaceae having two ascosporic genera, Lipomyces and Dipodascopsis, and the anamorphic genus Myxozyma...
  27. ncbi The genome of wine yeast Dekkera bruxellensis provides a tool to explore its food-related properties
    Jure Piskur
    Wine Research Centre, University of Nova Gorica, Nova Gorica, Slovenia
    Int J Food Microbiol 157:202-9. 2012
    ..cerevisiae lineage. However, D. bruxellensis has several independently duplicated ADH and ADH-like genes, which are likely responsible for metabolism of alcohols, including ethanol, and also a range of aromatic compounds...
  28. pmc Random UV-C mutagenesis of Scheffersomyces (formerly Pichia) stipitis NRRL Y-7124 to improve anaerobic growth on lignocellulosic sugars
    Stephen R Hughes
    Renewable Product Technology RPT Research Unit, National Center for Agricultural Utilization Research NCAUR, Agricultural Research Service ARS, United States Department of Agriculture USDA, Peoria, IL 61604, USA
    J Ind Microbiol Biotechnol 39:163-73. 2012
    ..The S. stipitis strains resulting from this intense multigene mutagenesis strategy have potential application in industrial fuel ethanol production from lignocellulosic hydrolysates...
  29. ncbi Co-evolution of transcriptional and post-translational cell-cycle regulation
    Lars Juhl Jensen
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Nature 443:594-7. 2006
    ....
  30. ncbi The DNA replication checkpoint response stabilizes stalled replication forks
    M Lopes
    Istituto F.I.R.C. di Oncologia Molecolare, Via Serio 21, 20141, Milano, Italy
    Nature 412:557-61. 2001
    ..Further, Rad53 is required to properly maintain stable replication forks during the block. We propose that Rad53 prevents collapse of the fork when replication pauses...
  31. ncbi First isolation of Stephanoascus ciferrii from a cat
    R Kano
    Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan
    Microbiol Immunol 44:711-3. 2000
    ..Therefore, the isolate was identified as Stephanoascus ciferrii, confirming the result of mycological examination by molecular analysis...
  32. ncbi Phylogenetic placement of Hanseniaspora-Kloeckera species using multigene sequence analysis with taxonomic implications: descriptions of Hanseniaspora pseudoguilliermondii sp. nov. and Hanseniaspora occidentalis var. citrica var. nov
    Neza Cadez
    University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology, Jamnikarjeva 101, 1000 Ljubljana, Slovenia
    Int J Syst Evol Microbiol 56:1157-65. 2006
    ..occidentalis var. occidentalis (type strain CBS 2592T) and H. occidentalis var. citrica var. nov. (type strain CBS 6783T), which could be distinguished by habitat preference...
  33. pmc The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast
    S Biggins
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    Genes Dev 13:532-44. 1999
    ..Because aurora2 has been implicated in oncogenesis, defects in kinetochore function may contribute to genetic instability in human tumors...
  34. ncbi Evidence that a septin diffusion barrier is dispensable for cytokinesis in budding yeast
    Carsten Wloka
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Biol Chem 392:813-29. 2011
    ....
  35. ncbi Monitoring the cell cycle by multi-kinase-dependent regulation of Swe1/Wee1 in budding yeast
    Kyung S Lee
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cell Cycle 4:1346-9. 2005
    ..Thus, Swe1 functions as an important cell cycle modulator that integrates multiple upstream signals from prior cell cycle events before its ultimate degradation permits passage into mitosis...
  36. ncbi Delimitation of the species of the Debaryomyces hansenii complex by intron sequence analysis
    Noémie Jacques
    CIRM Levures, Laboratoire de Microbiologie et Genetique Moleculaire, INRA, CNRS, AgroParisTech, 78850 Thiverval Grignon, France
    Int J Syst Evol Microbiol 59:1242-51. 2009
    ..hansenii and genetic exchanges between these populations, indicating an unexpected genetic diversity within this part of the genus Debaryomyces...
  37. ncbi Chromosomal polymorphism in the yeast species Debaryomyces hansenii
    Mauricio Corredor
    Laboratoire de Génétique Moléculaire et Cellulaire INRA UMR216, CNRS URA1925, Collection de Levures d Intérêt Biotechnologique, Institut National Agronomique Paris Grignon, 78850 Thiverval Grignon, France
    Antonie Van Leeuwenhoek 83:215-22. 2003
    ..hansenii var. hansenii CBS767T. These probes failed to hybridize to D. hansenii var. fabryii strongly suggesting that strains of this variety actually represent a different taxon...
  38. pmc Actin depolymerization drives actomyosin ring contraction during budding yeast cytokinesis
    Inês Mendes Pinto
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Dev Cell 22:1247-60. 2012
    ..elegans embryonic cells. This prediction was validated in yeast cells of different sizes due to different ploidies...
  39. pmc Direct evidence for a critical role of myosin II in budding yeast cytokinesis and the evolvability of new cytokinetic mechanisms in the absence of myosin II
    Nicola Tolliday
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Biol Cell 14:798-809. 2003
    ..Taken together, these results strongly suggest that the actomyosin ring is crucial for successful cytokinesis in budding yeast, but new cytokinetic mechanisms can evolve through genetic changes when myosin II function is impaired...
  40. ncbi Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales
    Cletus P Kurtzman
    Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL, USA
    FEMS Yeast Res 10:353-61. 2010
    ..Ogataea and neighboring taxa (Phylum Ascomycota, Subphylum Saccharomycotina, Class Saccharomycetes, Order Saccharomycetales) was determined from phylogenetic analyses of gene sequences for nuclear large and small subunit (SSU) rRNAs,..
  41. pmc Draft genome sequence of the yeast Pachysolen tannophilus CBS 4044/NRRL Y-2460
    Xiaoying Liu
    Center for Systems Microbiology, Department of Systems Biology, Matematiktorvet, Technical University of Denmark, Kongens Lyngby, Denmark
    Eukaryot Cell 11:827. 2012
    ..The sequenced genome size was 12,238,196 bp, consisting of 34 scaffolds. A total of 4,463 genes from 5,346 predicted open reading frames were annotated with function...
  42. pmc S-phase checkpoint genes safeguard high-fidelity sister chromatid cohesion
    Cheryl D Warren
    McKusick Nathans Institute of Genetic Medicine, Ross 850, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Biol Cell 15:1724-35. 2004
    ..This subset is particularly enriched for genes that support the S-phase checkpoint. We suggest that these genes promote and protect a chromatin environment conducive to robust cohesion...
  43. pmc EXO1 plays a role in generating type I and type II survivors in budding yeast
    Laura Maringele
    School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    Genetics 166:1641-9. 2004
    ..We show that EXO1 is important for generating type I survivors in yku70delta mre11delta cells and type II survivors in tlc1delta cells. Moreover, in tlc1delta cells, EXO1 seems to contribute to the senescence process itself...
  44. pmc Yeast Pch2 promotes domainal axis organization, timely recombination progression, and arrest of defective recombinosomes during meiosis
    G Valentin Börner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 105:3327-32. 2008
    ..We further propose that regulatory surveillance of normal and abnormal interchromosomal interactions in mitotic and meiotic cells may involve "structure-dependent interchromosomal interaction" (SDIX) checkpoints...
  45. ncbi Predicting protein localization in budding yeast
    Kuo Chen Chou
    Gordon Life Science Institute San Diego, CA 92130, USA
    Bioinformatics 21:944-50. 2005
    ..The present study was initiated in an attempt to address (1) how to efficiently identify the localization of a query protein among many possible subcellular locations, and (2) how to deal with the case of multiplex locations...
  46. ncbi The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome
    Fred S Dietrich
    Biozentrum der Universitat Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland
    Science 304:304-7. 2004
    ..cerevisiae included a whole genome duplication or fusion of two related species and showed, through inferred ancient gene orders, which of the duplicated genes lost one copy and which retained both copies...
  47. pmc Identification of cis-acting sites for condensin loading onto budding yeast chromosomes
    Claudio D'Ambrosio
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    Genes Dev 22:2215-27. 2008
    ..This reveals that TFIIIC-binding sites, including tRNA genes, constitute a hitherto unknown chromosomal feature with important implications for chromosome architecture during both interphase and mitosis...
  48. ncbi The replication fork block protein Fob1 functions as a negative regulator of the FEAR network
    Frank Stegmeier
    Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17 233, 40 Ames St, Cambridge, MA 02139, USA
    Curr Biol 14:467-80. 2004
    ..The Cdc14 early anaphase release (FEAR) network promotes activation of the phosphatase during early anaphase, whereas the mitotic exit network (MEN) activates Cdc14 during late stages of anaphase...
  49. pmc Uncovering novel cell cycle players through the inactivation of securin in budding yeast
    Sumeet Sarin
    The Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0840, USA
    Genetics 168:1763-71. 2004
    ..Using a modified synthetic genetic array (SGA) screen for genes that become essential in the absence of Pds1p, we uncovered roles for uncharacterized genes in cell cycle processes, including Esp1p activation...
  50. pmc Fragilities caused by dosage imbalance in regulation of the budding yeast cell cycle
    Kazunari Kaizu
    Department of Science and Technology, Keio University, Yokohama shi, Kanagawa, Japan
    PLoS Genet 6:e1000919. 2010
    ..The masking function of CLB2 was stabilization of Pds1 by its phosphorylation. We finally modified Chen's model according to our findings. We thus propose that dosage imbalance causes fragility in biological systems...
  51. pmc The septins function in G1 pathways that influence the pattern of cell growth in budding yeast
    Thea A Egelhofer
    Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America
    PLoS ONE 3:e2022. 2008
    ..Phosphorylation of Shs1 by G1 cyclin-dependent kinases plays a role in events that influence Cdk1 inhibitory phosphorylation...
  52. ncbi Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling
    Don W Cleveland
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Cell 112:407-21. 2003
    ....
  53. pmc Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast
    A F Straight
    Department of Physiology, School of Medicine, University of California San Francisco, San Francisco, California 94143, USA
    J Cell Biol 143:687-94. 1998
    ..The kip3Delta and kip1Delta mutants affect the duration of anaphase, but cin8Delta does not...
  54. ncbi Topoisomerase II inactivation prevents the completion of DNA replication in budding yeast
    Jonathan Baxter
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Mol Cell 30:790-802. 2008
    ....
  55. ncbi Catalytic properties of D-amino acid oxidase in cephalosporin C bioconversion: a comparison between proteins from different sources
    Loredano Pollegioni
    Department of Structural and Functional Biology, University of Insubria, via J H Dunant 3, 21100 Varese, Italy
    Biotechnol Prog 20:467-73. 2004
    ..gracilis enzyme in the free form. In any case, for industrial purposes DAAO is used only in the immobilized form where a strong enzyme stabilization occurs...
  56. pmc Telomerase- and Rad52-independent immortalization of budding yeast by an inherited-long-telomere pathway of telomeric repeat amplification
    Nathalie Grandin
    UMR CNRS no 5239, Ecole Normale Superieure de Lyon, IFR128 BioSciences Gerland, 69364 Lyon, France
    Mol Cell Biol 29:965-85. 2009
    ..Possible mechanisms for this Rad52-independent pathway of telomeric repeat amplification are discussed. The effects of inherited long telomeres on Rad52-dependent recombination are also reported...
  57. pmc Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii
    Sophie Brachat
    Institute of Applied Microbiology, Biozentrum der Universitat Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    Genome Biol 4:R45. 2003
    ..Thus, it became possible to reinvestigate the S. cerevisiae genome in the syntenic regions leading to an improved annotation...
  58. ncbi Synchronized dynamics and non-equilibrium steady states in a stochastic yeast cell-cycle network
    Hao Ge
    School of Mathematical Sciences, Peking University, Beijing, PR China
    Math Biosci 211:132-52. 2008
    ..Such a large separation in the magnitude of the circulations, between a dominant, main cycle and the rest, gives rise to the stochastic synchronization phenomenon...
  59. pmc Simple centromere, complex kinetochore: linking spindle microtubules and centromeric DNA in budding yeast
    Iain M Cheeseman
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA
    J Cell Biol 157:199-203. 2002
    ..This analysis may provide general principles for kinetochore function and regulation...
  60. pmc DNA replication forks pause at silent origins near the HML locus in budding yeast
    Y Wang
    Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
    Mol Cell Biol 21:4938-48. 2001
    ..Possible functions for replication fork pause sites in checkpoints, S-phase regulation, mating-type switching, and transcriptionally silent heterochromatin are discussed...
  61. ncbi Application of waste activated bleaching earth containing rapeseed oil on riboflavin production in the culture of Ashbya gossypii
    H Ming
    Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422 8529, Japan
    Biotechnol Prog 19:410-7. 2003
    ..The waste ABE containing waste vegetable oil was suitable for raw material for production of the value-added useful bioproducts, which might be a good model for reuse of the waste resource...
  62. ncbi The spindle checkpoint rescues the meiotic segregation of chromosomes whose crossovers are far from the centromere
    Soni Lacefield
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Nat Genet 39:1273-7. 2007
    ..The tether partially rescues the segregation of chromosomes that lack crossovers...
  63. pmc Combined analysis reveals a core set of cycling genes
    Yong Lu
    Department of Computer Science, Carnegie Mellon University, Forbes Avenue, Pittsburgh, Pennsylvania 15213, USA
    Genome Biol 8:R146. 2007
    ..Combining and comparing data from multiple species is challenging because of noise in expression data, the different synchronization and scoring methods used, and the need to determine an accurate set of homologs...
  64. ncbi Cdk1 coordinates cell-surface growth with the cell cycle
    Derek McCusker
    Department of Molecular, Cell and Developmental Biology, University of California, 1156 High Street, Santa Cruz, CA 95064, USA
    Nat Cell Biol 9:506-15. 2007
    ..A mutant form of a Cdc24-associated protein that fails to undergo Cdk1-dependent phosphorylation causes defects in bud growth. These results provide a direct link between Cdk1 activity and the control of polarized cell growth...
  65. pmc Mps1 kinase promotes sister-kinetochore bi-orientation by a tension-dependent mechanism
    Jean François Maure
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, MSI WTB JBC Complex, Dundee DD1 5EH, UK
    Curr Biol 17:2175-82. 2007
    ..We did not find evidence that Ipl1 regulates Mps1 or vice versa; therefore, they play similar, but possibly independent, roles in facilitating bi-orientation...
  66. ncbi Isolation of Ashbya gossypii mutant for an improved riboflavin production targeting for biorefinery technology
    E Y Park
    Laboratory of Biotechnology, Major in Bioscience, Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan
    J Appl Microbiol 103:468-76. 2007
    ..To isolate a strain overproducing riboflavin and to improve riboflavin production for practical use in a biorefinery technology...
  67. pmc Cytokinesis depends on the motor domains of myosin-II in fission yeast but not in budding yeast
    Matthew Lord
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Mol Biol Cell 16:5346-55. 2005
    ..Myo1p is the first example of a myosin whose cellular function does not require a catalytic motor domain revealing a novel mechanism of action for budding yeast myosin-II independent of actin binding and ATPase activity...
  68. pmc A Ras-like GTPase is involved in hyphal growth guidance in the filamentous fungus Ashbya gossypii
    Yasmina Bauer
    Department of Molecular Microbiology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Mol Biol Cell 15:4622-32. 2004
    ..Additionally, hyphal filaments in the mutant developed aberrant branching sites by mislocalizing AgSpa2p thus distorting hyphal morphology. These results define AgRsr1p/Bud1p as a key regulator of hyphal growth guidance...
  69. ncbi Bifurcation analysis of a model of the budding yeast cell cycle
    Dorjsuren Battogtokh
    Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
    Chaos 14:653-61. 2004
    ..In a three-variable model exhibiting birhythmicity (two stable oscillatory states), we explore possible effects of extrinsic fluctuations on cell cycle progression...
  70. pmc Metabolic engineering of the purine pathway for riboflavin production in Ashbya gossypii
    Alberto Jimenez
    Departamento de Microbiologia y Genetica, CSIC Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain
    Appl Environ Microbiol 71:5743-51. 2005
    ..With these manipulations, we managed to enhance metabolic flow through the purine pathway and to increase the production of riboflavin in the triple mutant strain 10-fold (228 mg/liter)...
  71. ncbi Crm1-mediated nuclear export of Cdc14 is required for the completion of cytokinesis in budding yeast
    Joshua Bembenek
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell Cycle 4:961-71. 2005
    ..Our results suggest a requirement for Crm1p-dependent nuclear export of Cdc14p in coordinating mitotic exit and cytokinesis in budding yeast...
  72. pmc Chromosome bi-orientation on the mitotic spindle
    Tomoyuki U Tanaka
    School of Life Sciences, University of Dundee, Wellcome Trust Biocentre, Dow Street, Dundee DD1 5EH, Scotland, UK
    Philos Trans R Soc Lond B Biol Sci 360:581-9. 2005
    ..In this article, I discuss how cells ensure sister kinetochore bi-orientation for all chromosomes, mainly focusing on our recent findings in budding yeast...
  73. pmc Forced periodic expression of G1 cyclins phase-locks the budding yeast cell cycle
    G Charvin
    Laboratory of Yeast Molecular Genetics and Center for Studies in Physics and Biology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 106:6632-7. 2009
    ..Our results provide quantitative insight into how cell size is integrated with the cell cycle oscillator...
  74. ncbi Mitotic-exit control as an evolved complex system
    William J Bosl
    University of California, Davis Cancer Center, Sacramento, CA 95817, USA
    Cell 121:325-33. 2005
    ..We focus on four particularly intriguing features of the mitotic-exit control system and attempt to examine these features from the perspective of evolutionary design and complex system engineering...
  75. ncbi Thermal inactivation of D-amino acid oxidase from Trigonopsis variabilis occurs via three parallel paths of irreversible denaturation
    Iskandar Dib
    Research Centre Applied Biocatalysis and Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, A 8010 Graz, Austria
    Biotechnol Bioeng 94:645-54. 2006
    ..03/h). The results fully explain the different stabilities of native and oxidized TvDAO and provide an inactivation mechanism-based tool for the stabilization of the soluble oxidase...
  76. ncbi Modular logical modelling of the budding yeast cell cycle
    Adrien Fauré
    Université de la Méditerranée and INSERM U928 TAGC, Marseille, France
    Mol Biosyst 5:1787-96. 2009
    ..The resulting logical model preserves all relevant characteristics of the original modules, while enabling the simulation of more sophisticated experiments...
  77. pmc The Ashbya Genome Database (AGD)--a tool for the yeast community and genome biologists
    Leandro Hermida
    Department of Applied Microbiology, Biozentrum, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Nucleic Acids Res 33:D348-52. 2005
    ..AGD is accessible at http://agd.unibas.ch/...
  78. pmc Trigonopsis variabilis D-amino acid oxidase: control of protein quality and opportunities for biocatalysis through production in Escherichia coli
    Iskandar Dib
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12 I, A 8010 Graz, Austria
    Appl Environ Microbiol 73:331-3. 2007
    ..The purified oxidase lacks the microheterogeneity of the natural enzyme. Oriented immobilization of a chimeric oxidase maintains 80% of the original activity in microparticle-bound enzymes...
  79. ncbi Unexpected complexity of the budding yeast transcriptome
    Takashi Ito
    Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa 277 8561, Japan
    IUBMB Life 60:775-81. 2008
    ..The budding yeast may serve as an excellent model to study pervasive transcription and noncoding RNAs...
  80. pmc Telomerase- and recombination-independent immortalization of budding yeast
    Laura Maringele
    University of Newcastle, School of Clinical Medical Sciences Gerontology, Newcastle upon Tyne, NE4 6BE, United Kingdom
    Genes Dev 18:2663-75. 2004
    ..For the first time, we show that large palindromes form as a natural consequence of postsenescence growth and that they become essential for immortalization in the absence of telomerase activity...
  81. ncbi S-Cdk-dependent phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast
    Hiroshi Masumoto
    Division of Microbial Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Yata 1111, Mishima, Shizuoka 411 8540, Japan
    Nature 415:651-5. 2002
    ..Because this complex is suggested to be essential for chromosomal DNA replication, it seems likely that S-Cdk positively regulates formation of the Sld2-Dpb11 complex and, consequently, chromosomal DNA replication...
  82. ncbi The septation apparatus, a chitin-requiring machine in budding yeast
    Enrico Cabib
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health DHHS, Bethesda, MD 20892, USA
    Arch Biochem Biophys 426:201-7. 2004
  83. pmc MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p
    K G Hardwick
    Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    J Cell Biol 148:871-82. 2000
    ..We discuss roles for Mad3p and its interactions with other spindle checkpoint proteins and with Cdc20p, the target of the checkpoint...
  84. pmc Mechanisms controlling subcellular localization of the G(1) cyclins Cln2p and Cln3p in budding yeast
    M E Miller
    The Rockefeller University, New York, New York 10021, USA
    Mol Cell Biol 21:6292-311. 2001
    ....
  85. ncbi In vitro regulation of budding yeast Bfa1/Bub2 GAP activity by Cdc5
    Marco Geymonat
    Divisions of Yeast Genetics and Protein Structure, National Institute for Medical Research, Mill Hill, London NW7 1AA, Great Britain
    J Biol Chem 278:14591-4. 2003
    ..Thus, in vivo inactivation of Bfa1-Bub2 by Cdc5 would have a positive regulatory effect by increasing levels of Tem1-GTP so stimulating exit from mitosis...
  86. ncbi Regulation and properties of a fungal lipase showing interfacial inactivation by gas bubbles, or droplets of lipid or fatty acid
    K P Stahmann
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Germany
    Eur J Biochem 244:220-5. 1997
    ..Inactivation and regulation thus explained the lack of detectable lipase activity in cultures of A. gossypii growing on triacylglycerol...
  87. pmc Chl4p and iml3p are two new members of the budding yeast outer kinetochore
    Isabelle Pot
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada V5Z 4H4
    Mol Biol Cell 14:460-76. 2003
    ..These physical interaction dependencies provide insights into the molecular architecture and centromere DNA loading requirements of the outer kinetochore complex...
  88. pmc Modes of spindle pole body inheritance and segregation of the Bfa1p-Bub2p checkpoint protein complex
    G Pereira
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
    EMBO J 20:6359-70. 2001
    ..This may provide a mechanism to delay cell cycle progression when cytoplasmic microtubules fail to orient the spindle. Thus, SPBs are able to sense cytoplasmic microtubule properties and regulate the Bfa1p-Bub2p GAP accordingly...
  89. ncbi Cell polarity and morphogenesis in budding yeast
    K Madden
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Annu Rev Microbiol 52:687-744. 1998
    ..Since many of the components important for polarized cell growth are conserved in other organisms, the basic mechanisms mediating polarized cell growth are likely to be universal among eukaryotes...
  90. pmc Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complex
    A D Rudner
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    J Cell Biol 149:1377-90. 2000
    ..We show that, like cdc28 mutants, cdc5 mutants affect APC phosphorylation in vivo. However, although Cdc5 can phosphorylate Cdc16 and Cdc27 in vitro, this in vitro phosphorylation does not occur on in vivo sites of phosphorylation...
  91. ncbi Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p
    Iain M Cheeseman
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 111:163-72. 2002
    ..Our systematic mutational analysis of the Ipl1p phosphorylation sites demonstrated that the essential microtubule binding protein Dam1p is a key Ipl1p target for regulating kinetochore-microtubule attachments in vivo...
  92. ncbi Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production
    K P Stahmann
    Institut für Biotechnologie 1, Forschungszentrum Julich, Germany
    Appl Microbiol Biotechnol 53:509-16. 2000
    ..Multiple copies of the rib genes and promoter replacements are necessary to achieve competitive productivity...
  93. pmc Disruption of the SHM2 gene, encoding one of two serine hydroxymethyltransferase isoenzymes, reduces the flux from glycine to serine in Ashbya gossypii
    Christina Schlüpen
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, D 52425 Julich, Germany
    Biochem J 369:263-73. 2003
    ..Knockout of SHM 1 had no detectable effect on serine labelling, but disruption of SHM 2 led to a decrease in serine (2-5%) and an increase in glycine (59-67%) labelling, indicating a changed carbon flux...
  94. ncbi Cytokinesis in budding yeast: the relationship between actomyosin ring function and septum formation
    E Bi
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia 19104 6058, USA
    Cell Struct Funct 26:529-37. 2001
    ..Here we review and provide additional evidence that defines the functional and molecular relationship between actomyosin ring function and septum formation...
  95. ncbi The core meiotic transcriptome in budding yeasts
    M Primig
    The University of Chicago, Department of Molecular Genetics and Cell Biology, Chicago, Illinois, USA
    Nat Genet 26:415-23. 2000
    ..The transcript profiles correlate with the distribution of defined meiotic promoter elements and with the time of known gene function...
  96. ncbi Hiding at the ends of yeast chromosomes: telomeres, nucleases and checkpoint pathways
    David Lydall
    School of Biological Sciences, University of Manchester, G38 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    J Cell Sci 116:4057-65. 2003
    ..A model for how a spectrum of telomeric states might interact with telomerase and checkpoint pathways is proposed...
  97. ncbi Lack of tension at kinetochores activates the spindle checkpoint in budding yeast
    B M Stern
    Department of Molecular and Cell Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Curr Biol 11:1462-7. 2001
    ..Because the spindle checkpoint is activated in these cells, we conclude that the absence of tension at the yeast kinetochore is sufficient to activate the spindle checkpoint in mitosis...
  98. ncbi Regulation of septin organization and function in yeast
    Mark S Longtine
    Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74075 3035, USA
    Trends Cell Biol 13:403-9. 2003
    ..Septins are thought to function in diverse processes in S. cerevisiae, mainly by acting as a scaffold to direct the neck localization of septin-associated proteins...
  99. pmc Mdm20 protein functions with Nat3 protein to acetylate Tpm1 protein and regulate tropomyosin-actin interactions in budding yeast
    Jason M Singer
    Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 100:7644-9. 2003
    ..These combined results strongly suggest that Mdm20p-dependent, N-terminal acetylation of Tpm1p by the NatB complex is required for Tpm1p association with, and stabilization of, actin filaments and cables...
  100. ncbi Use of physicochemical tools to determine the choice of optimal enzyme: stabilization of D-amino acid oxidase
    Lorena Betancor
    Laboratorio de Tecnologia Enzimatica, Departamento de Biocatalisis, ICP CSIC, Campus UAM, Cantoblanco, 28049 Madrid, Spain
    Biotechnol Prog 19:784-8. 2003
    ..Therefore, the most stable DAAO preparation and the optimal choice for an industrial application seems to be RgDAAO immobilized on glyoxyl agarose...
  101. ncbi Telomere maintenance without telomerase
    Victoria Lundblad
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, TX 77030, USA
    Oncogene 21:522-31. 2002
    ..These observations provide new insights into the mechanism(s) by which recombination can restore telomere function in yeast, and suggest future experiments for the investigation of potentially similar pathways in human cells...

Research Grants31

  1. Architecture of the Diffusion Barrier in the Nuclear Pore Complex
    Michael F Rexach; Fiscal Year: 2010
    ..When they fail to function normally, these proteins can trigger the onset of cancer. We wish to understand how their structure enables them to function as gatekeepers of the nucleus. ..
  2. Development and validation of predictors of endocrine treatment sensitivity
    Christos Hatzis; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  3. A Strain Repository for Dictyostelium discoideum
    RICHARD KESSIN; Fiscal Year: 2008
    ..The Stock Center will continue to increase the efficiency of research and to guarantee the security of availability of biological resources. [unreadable] [unreadable] [unreadable]..
  4. How Karyopherins Move Across The Nuclear Pore Complex
    Michael Rexach; Fiscal Year: 2006
    ..abstract_text> ..
  5. Molecular genetics of translational accuracy
    Philip Farabaugh; Fiscal Year: 2007
    ..This work will be done both in bacteria and in yeast. We will also study which trans-acting proteins interact with this accuracy mechanism by studying the accuracy effect of a class of candidate gene deletions in yeast. ..
  6. HOMOLOGOUS RECOMBINATION IN MAMMALIAN CELLS
    ROBERT LISKAY; Fiscal Year: 2006
    ..Finally, we anticipate identifying additional proteins involved in MMR-related processes, which may have functions in preventing human disease such as cancer. ..
  7. FASEB Summer Conference on Transcriptional Regulation During Cell Growth
    Jessica Tyler; Fiscal Year: 2008
    ..As transcription is the ultimate endpoint for many regulatory processes an understanding of its mechanism will have profound implications for human health. [unreadable] [unreadable] [unreadable]..
  8. The Function of Dynein in Budding Yeast
    John Cooper; Fiscal Year: 2009
    ..We will focus on defining molecular mechanisms for these processes and understanding how they are regulated. ..
  9. LASER SCANNING CONFOCAL IMAGING SYSTEM
    Liza Pon; Fiscal Year: 2003
    ..I. As an integral component of our Optical Microscopy Facility, the instrument will be accessible to members of the user group and others throughout the University, promoting collaboration and exchange of ideas. ..
  10. Septins and the Cell Cycle in Budding Yeast
    John Cooper; Fiscal Year: 2009
    ..To test this hypothesis, we will examine septin filament architecture with respect to the cell cycle and checkpoint activation. ..
  11. Developmental control of cell cycle exit
    LINDA BREEDEN; Fiscal Year: 2009
    ....
  12. CELL CYCLE REGULATION OF THE YEAST HO GENE
    Linda L Breeden; Fiscal Year: 2010
    ..We will use this method to test a series of mutants that deregulate the mitotic start program to see how GO to S and G1 to S differ. ..
  13. ANALYSIS OF TELOMERASE REVERSE TRANSCRIPTASE
    Neal F Lue; Fiscal Year: 2010
    ....
  14. Regulation of Replication Origin Usage in Saccharomyces cerevisiae
    Bik Kwoon Tye; Fiscal Year: 2009
    ..Therefore, understanding the regulation of DNA replication is central to the study of the etiology of all genetic diseases rooted in genome instability including trisomy and cancer. ..
  15. SHARED HIGH RESOLUTION MULTIMODE MICROSCOPE SYSTEM
    Greenfield Sluder; Fiscal Year: 2001
    ..The proposed instrument system will allow these researchers to take their investigations in new directions, obtain novel information, and thus, significantly advance their programs in basic biomedical research. ..
  16. Telomerase function and regulation in a new model system
    Neal Lue; Fiscal Year: 2007
    ..We anticipate that our findings will contribute to a detailed molecular understanding of telomerase function and regulation. ..
  17. MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLY
    Lynne Cassimeris; Fiscal Year: 2006
    ..To address these issues, we will apply a number of fluorescence-based imaging methods combined with siRNA or injection of function-blocking proteins or antibodies. ..
  18. MECHANISM OF ANAPHASE IN EUKARYOTIC CELLS
    David Pellman; Fiscal Year: 2001
    ....
  19. ROLE OF UBIQUITIM-LIKE PROTEINS IN DICTYOSTELIU
    RICHARD KESSIN; Fiscal Year: 2002
    ..One explanation for the effect of nosA mutation is that NosA and SonA are part of the mechanism which Dictyostelium amoebae use to arrest their cell cycles or sense starvation when they enter development. ..
  20. INTERACTIONS OF THE MCM PROTEINS AT REPLICATION ORIGINS
    Bik Kwoon Tye; Fiscal Year: 2002
    ..Through understanding of normal cellular processes that regulate DNA replication, it may be possible to elucidate the molecular basis for abnormalities or defects that lead to the disease state in cancer cells. ..
  21. Epigenetic Regulation of Mitochondiral Complex II
    Gerald S Shadel; Fiscal Year: 2010
    ..Understanding the molecular basis of imprinting in PGL may provide general insights on how differential expression of a single defective gene can lead to disease or normalcy. ..
  22. Actin Organization and Polarization
    Liza Pon; Fiscal Year: 2005
    ..The studies proposed are designed to fill that gap. ..
  23. MOLECULAR GENETIC BASIS OF HUMAN DISEASE
    ROBERT LISKAY; Fiscal Year: 2006
    ..The variety of opportunities available to the students allows the program to meet the goal of preparing young investigators for careers in biomedical research with heavy emphasis on the study of normal and abnormal genetic mechanisms. ..
  24. Molecular Analysis of the Kinetochore
    SONI SHIMODA; Fiscal Year: 2006
    ..A characterization of the proteins involved in chromosome segregation and checkpoint activation could lead to a greater understanding of how human diseases, such as cancer, can be prevented. [unreadable] [unreadable]..
  25. High through-put detection of fungal pathogens
    JACK FELL; Fiscal Year: 2004
    ..Resulting data will be disseminated via GenBank, publications in leading scientific journals and as an inexpensive commercial detection system. ..
  26. Molecular genetics of translational accuracy
    PHILIP JAMES FARABAUGH; Fiscal Year: 2010
    ..The conclusion of our work is that wobble errors commonly occur, tllOugh they are not universal, and that other types of errors occur when the competing ..
  27. RECOMBINATION MECHANISMS IN YEAST CELL DIFFERENTIATION
    James Haber; Fiscal Year: 2006
    ..abstract_text> ..
  28. POSTTRANSCRIPTIONAL GENE REGULATION IN T CELL ACTIVATION
    JACK KEENE; Fiscal Year: 2004
    ..In summary, HuR will be examined for RNA recognition specificity and interactions, which govern its effects on the stability or translation of cytokine and ERG mRNAs during T cell activation. ..
  29. Cancer markers from bimodally expressed genes
    Christos Hatzis; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..