bacterial spores

Summary

Summary: Heat and stain resistant, metabolically inactive bodies formed within the vegetative cells of bacteria of the genera Bacillus and Clostridium.

Top Publications

  1. ncbi Spore germination
    Peter Setlow
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    Curr Opin Microbiol 6:550-6. 2003
  2. ncbi Anthrax
    M Mock
    Toxines et Pathogénie Bactérienne, CNRS URA 2172, Institut Pasteur, Paris Cedex 15, France
    Annu Rev Microbiol 55:647-71. 2001
  3. ncbi The Spo0A regulon of Bacillus subtilis
    Virginie Molle
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Microbiol 50:1683-701. 2003
  4. ncbi Anthrax as a biological weapon, 2002: updated recommendations for management
    Thomas V Inglesby
    Johns Hopkins Center for Civilian Biodefense Strategies, Johns Hopkins University, Baltimore, MD 21202, USA
    JAMA 287:2236-52. 2002
  5. ncbi Bistability in bacteria
    David Dubnau
    Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Mol Microbiol 61:564-72. 2006
  6. ncbi Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression
    P Stragier
    Institut de Microbiologie, Universite Paris Sud, Orsay, France
    Cell 52:697-704. 1988
  7. ncbi Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals
    P Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, 06030 3305, USA
    J Appl Microbiol 101:514-25. 2006
  8. pmc Investigation of bioterrorism-related anthrax, United States, 2001: epidemiologic findings
    Daniel B Jernigan
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Emerg Infect Dis 8:1019-28. 2002
  9. ncbi A comparative genomic view of clostridial sporulation and physiology
    Carlos J Paredes
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, USA
    Nat Rev Microbiol 3:969-78. 2005
  10. pmc Northern, morphological, and fermentation analysis of spo0A inactivation and overexpression in Clostridium acetobutylicum ATCC 824
    Latonia M Harris
    Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208, USA
    J Bacteriol 184:3586-97. 2002

Detail Information

Publications248 found, 100 shown here

  1. ncbi Spore germination
    Peter Setlow
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    Curr Opin Microbiol 6:550-6. 2003
    ..subtilis, and completion of cortex hydrolysis and subsequent germ cell wall expansion allows full spore core hydration and resumption of spore metabolism and macromolecular synthesis...
  2. ncbi Anthrax
    M Mock
    Toxines et Pathogénie Bactérienne, CNRS URA 2172, Institut Pasteur, Paris Cedex 15, France
    Annu Rev Microbiol 55:647-71. 2001
    ..Spore-structure analysis could contribute to the improvement of human nonliving vaccines. The phylogeny of B. anthracis within the Bacillus cereus group is also reviewed...
  3. ncbi The Spo0A regulon of Bacillus subtilis
    Virginie Molle
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Microbiol 50:1683-701. 2003
    ..Spo0A is a master regulator for sporulation, but many of its effects on the global pattern of gene transcription are likely to be mediated indirectly by regulatory genes under its control...
  4. ncbi Anthrax as a biological weapon, 2002: updated recommendations for management
    Thomas V Inglesby
    Johns Hopkins Center for Civilian Biodefense Strategies, Johns Hopkins University, Baltimore, MD 21202, USA
    JAMA 287:2236-52. 2002
    ..To review and update consensus-based recommendations for medical and public health professionals following a Bacillus anthracis attack against a civilian population...
  5. ncbi Bistability in bacteria
    David Dubnau
    Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Mol Microbiol 61:564-72. 2006
    ..A review describing additional examples of bistability in bacteria has been published recently...
  6. ncbi Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression
    P Stragier
    Institut de Microbiologie, Universite Paris Sud, Orsay, France
    Cell 52:697-704. 1988
    ..According to this model, a morphological structure would directly control the synthesis of a developmental sigma factor and would modify gene expression...
  7. ncbi Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals
    P Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, 06030 3305, USA
    J Appl Microbiol 101:514-25. 2006
    ..Given the importance of the killing of spores of Bacillus species in the food and medical products industry, a deeper understanding of the mechanisms of spore resistance and killing may lead to improved methods for spore destruction...
  8. pmc Investigation of bioterrorism-related anthrax, United States, 2001: epidemiologic findings
    Daniel B Jernigan
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Emerg Infect Dis 8:1019-28. 2002
    ..Continued vigilance for cases is needed among health-care providers and members of the public health and law enforcement communities...
  9. ncbi A comparative genomic view of clostridial sporulation and physiology
    Carlos J Paredes
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, USA
    Nat Rev Microbiol 3:969-78. 2005
    ....
  10. pmc Northern, morphological, and fermentation analysis of spo0A inactivation and overexpression in Clostridium acetobutylicum ATCC 824
    Latonia M Harris
    Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208, USA
    J Bacteriol 184:3586-97. 2002
    ....
  11. ncbi Developmental cheating in the social bacterium Myxococcus xanthus
    G J Velicer
    Department of Biochemistry, Michigan State University, East Lansing 48824, USA
    Nature 404:598-601. 2000
    ..The ease of finding anti-social behaviours suggests that cheaters may be common in natural populations of M. xanthus...
  12. ncbi Spore UV and acceleration resistance of endolithic Bacillus pumilus and Bacillus subtilis isolates obtained from Sonoran desert basalt: implications for lithopanspermia
    James N Benardini
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona, USA
    Astrobiology 3:709-17. 2003
    b>Bacterial spores have been used as model systems for studying the theory of interplanetary transport of life by natural processes such as asteroidal or cometary impacts (i.e., lithopanspermia)...
  13. ncbi Recurrent isolation of hydrogen peroxide-resistant spores of Bacillus pumilus from a spacecraft assembly facility
    Michael J Kempf
    Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California 91109, USA
    Astrobiology 5:391-405. 2005
    ..pumilus in a clean-room is a concern because their persistence might potentially compromise life-detection missions, which have very strict cleanliness and sterility requirements for spacecraft hardware...
  14. ncbi Fatal inhalational anthrax in a 94-year-old Connecticut woman
    Lydia A Barakat
    Department of Infectious Diseases, Griffin Hospital, 130 Division St, Derby, CT 06418, USA
    JAMA 287:863-8. 2002
    ..The source of exposure to B anthracis in this patient is not known. Exposure to mail that was cross-contaminated as it passed through postal facilities contaminated with B anthracis spores is one hypothesis under investigation...
  15. pmc Do mycobacteria produce endospores?
    Bjorn A Traag
    The Biological Laboratories, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 107:878-81. 2010
    ..marinum. Finally, we failed to recover heat-resistant colony-forming units from frogs chronically infected with M. marinum. We conclude that it is unlikely that Mycobacterium is capable of endospore formation...
  16. ncbi The hidden lifestyles of Bacillus cereus and relatives
    G B Jensen
    National Institute of Occupational Health, Lersø Parkallé 105, 2100 Copenhagen, Denmark
    Environ Microbiol 5:631-40. 2003
    ....
  17. ncbi Sensitive and rapid quantitative detection of anthrax spores isolated from soil samples by real-time PCR
    Chunsun Ryu
    Research Center for Pathogen Control, National Institute of Health, 5 Rokbun dong, Eunpyung Gu, Seoul 122 701, Korea
    Microbiol Immunol 47:693-9. 2003
    ..As a consequence, we established a rapid and accurate detection system for environmental anthrax spores using real-time PCR, avoiding time and labor-consuming preparation steps such as enrichment culturing and DNA preparation...
  18. pmc High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis
    Masaya Fujita
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave, Cambridge, MA 02138, USA
    J Bacteriol 187:1357-68. 2005
    ....
  19. pmc Murine macrophages kill the vegetative form of Bacillus anthracis
    Tae Jin Kang
    Center for Vaccine Development, Department of Medicine, University of Maryland, 685 W Baltimore Street, HSF I 480, Baltimore, MD 21201, USA
    Infect Immun 73:7495-501. 2005
    ..We conclude that macrophages appear unable to kill the spore form of B. anthracis and that the exosporium may play a role in the protection of spores from macrophages...
  20. pmc Exogenous gamma and alpha/beta interferon rescues human macrophages from cell death induced by Bacillus anthracis
    Jeffrey A Gold
    Division of Pulmonary and Critical Care Medicine, Sackler Institute of Biomedical Studies, New York University School of Medicine, New York, New York 10016, USA
    Infect Immun 72:1291-7. 2004
    ..anthracis infection in macrophages are independent of LeTx. These data suggest an important role for both IFNs in the control of B. anthracis and the potential benefit of using exogenous IFN as an immunoadjuvant therapy...
  21. ncbi How do spores germinate?
    A Moir
    Department of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, Sheffield, UK
    J Appl Microbiol 101:526-30. 2006
    ..We do not yet know what activates SleB activity in the spore, and neither do we have any information at all on how the spore coat is degraded...
  22. ncbi Fatal inhalational anthrax with unknown source of exposure in a 61-year-old woman in New York City
    Bushra Mina
    Section of Critical Care Medicine, Department of Medicine, Lenox Hill Hospital, 100 E 77th St, New York, NY 10021, USA
    JAMA 287:858-62. 2002
    ..The cause of death was inhalational anthrax. Despite epidemiologic investigation, including environmental samples from the patient's residence and workplace, no mechanism for anthrax exposure has been identified...
  23. ncbi Comparison of the efficacy of a hydrogen peroxide dry-mist disinfection system and sodium hypochlorite solution for eradication of Clostridium difficile spores
    F Barbut
    National Reference Laboratory for Clostridium difficile, Hopital Saint Antoine, Assistance Publique Hopitaux de Paris, Paris
    Infect Control Hosp Epidemiol 30:507-14. 2009
    ..To compare a hydrogen peroxide dry-mist system and a 0.5% hypochlorite solution with respect to their ability to disinfect Clostridium difficile-contaminated surfaces in vitro and in situ...
  24. ncbi A bacteriolytic agent that detects and kills Bacillus anthracis
    Raymond Schuch
    Laboratory of Bacteriql Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021, USA
    Nature 418:884-9. 2002
    ..The lytic specificity of PlyG was also exploited as part of a rapid method for the identification of B. anthracis. We conclude that PlyG is a tool for the treatment and detection of B. anthracis...
  25. ncbi Asymptomatic carriers are a potential source for transmission of epidemic and nonepidemic Clostridium difficile strains among long-term care facility residents
    Michelle M Riggs
    Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH 44106, USA
    Clin Infect Dis 45:992-8. 2007
    ..Asymptomatic fecal carriage of Clostridium difficile is common in patients staying in health care facilities, but the importance of asymptomatic carriers with regard to disease transmission is unclear...
  26. pmc Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A
    Masaya Fujita
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 19:2236-44. 2005
    ..Evidence indicates that this gradual increase in Spo0A protein and activity plays a critical role in triggering sporulation and requires the action of the phosphorelay...
  27. pmc Sporulation in mycobacteria
    Jaydip Ghosh
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Uppsala SE 751 24, Sweden
    Proc Natl Acad Sci U S A 106:10781-6. 2009
    ..Together, our data show sporulation as a lifestyle adapted by mycobacteria under stress and tempt us to suggest this as a possible mechanism for dormancy and/or persistent infection. If so, this might lead to new prophylactic strategies...
  28. pmc Chenodeoxycholate is an inhibitor of Clostridium difficile spore germination
    Joseph A Sorg
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    J Bacteriol 191:1115-7. 2009
    ..Here we show that chenodeoxycholate inhibits the germination of C. difficile spores in response to cholate and taurocholate...
  29. pmc The transcriptional program underlying the physiology of clostridial sporulation
    Shawn W Jones
    Department of Chemical and Biological Engineering, Northwestern University, Sheridan Road, Evanston, IL 60208 3120, USA
    Genome Biol 9:R114. 2008
    ..Elucidation of their sporulation program is critical for understanding important clostridial programs pertaining to their physiology and their industrial or environmental applications...
  30. ncbi Efficacy of hospital cleaning agents and germicides against epidemic Clostridium difficile strains
    Warren N Fawley
    Department of Microbiology, General Infirmary, Old Medical School, Leeds, United Kingdom
    Infect Control Hosp Epidemiol 28:920-5. 2007
    ..To compare the effects of hospital cleaning agents and germicides on the survival of epidemic Clostridium difficile strains...
  31. ncbi Sporulation of Bacillus subtilis
    Patrick J Piggot
    Department of Microbiology and Immunology, Temple University School of Medicine 3400N Broad St, Philadelphia, Pennsylvania 19140, USA
    Curr Opin Microbiol 7:579-86. 2004
    ..There have been considerable advances in our understanding of critical controls that act to regulate the phosphorelay and to activate the sigma factors...
  32. ncbi Secondary aerosolization of viable Bacillus anthracis spores in a contaminated US Senate Office
    Christopher P Weis
    US Environmental Protection Agency National Enforcement Investigations Center, Denver Federal Center, Bldg 53, PO Box 25227, Denver, CO 80225, USA
    JAMA 288:2853-8. 2002
    ..Bioterrorist attacks involving letters and mail-handling systems in Washington, DC, resulted in Bacillus anthracis (anthrax) spore contamination in the Hart Senate Office Building and other facilities in the US Capitol's vicinity...
  33. pmc Characterization of bofA, a gene involved in intercompartmental regulation of pro-sigma K processing during sporulation in Bacillus subtilis
    E Ricca
    Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138
    J Bacteriol 174:3177-84. 1992
    ....
  34. pmc Validation of a nylon-flocked-swab protocol for efficient recovery of bacterial spores from smooth and rough surfaces
    Alexander Probst
    Institute of Microbiology and Archaea Center, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
    Appl Environ Microbiol 76:5148-58. 2010
    ..anthracis contamination for law enforcement and security efforts...
  35. pmc Anthrax spores make an essential contribution to vaccine efficacy
    Fabien Brossier
    Unité des Toxines et Pathogénie Bactérienne CNRS URA 2172, Institut Pasteur, 75725 Paris Cedex 15, France
    Infect Immun 70:661-4. 2002
    ..Guinea pigs and mice had very different susceptibilities to infection with the nontoxigenic strain, highlighting the importance of verifying the pertinence of animal models for evaluating anthrax vaccines...
  36. pmc spo0J is required for normal chromosome segregation as well as the initiation of sporulation in Bacillus subtilis
    K Ireton
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    J Bacteriol 176:5320-9. 1994
    ..The dual functions of Spo0J could provide a mechanism for regulating the initiation of sporulation in response to activity of the chromosome partition machinery...
  37. ncbi Generation of multiple cell types in Bacillus subtilis
    Daniel Lopez
    Department of Microbiology, Harvard Medical School, Boston, MA, USA
    FEMS Microbiol Rev 33:152-63. 2009
    ..While individual cells must choose distinct cell fates, the population as a whole exhibits a spectrum of phenotypes whose diversity may increase fitness...
  38. ncbi Temporal and spatial control of the mother-cell regulatory gene spoIIID of Bacillus subtilis
    B Kunkel
    Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138
    Genes Dev 3:1735-44. 1989
    ....
  39. pmc Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 and the hyper-butanol-producing mutant BA101 during the shift from acidogenesis to solventogenesis
    Zhen Shi
    Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Appl Environ Microbiol 74:7709-14. 2008
    ....
  40. ncbi The spoIIIA operon of Bacillus subtilis defines a new temporal class of mother-cell-specific sporulation genes under the control of the sigma E form of RNA polymerase
    N Illing
    Sir William Dunn School of Pathology, University of Oxford, UK
    Mol Microbiol 5:1927-40. 1991
    ..Since the onset of spoIIID expression occurs about 10 minutes after that of spoIIIA, spoIIIA expression is transient. Thus spoIIIA defines a third temporal class of gene controlled by the sigma E form of RNA polymerase...
  41. ncbi C-signal: a cell surface-associated morphogen that induces and co-ordinates multicellular fruiting body morphogenesis and sporulation in Myxococcus xanthus
    T Kruse
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Campusvej 55, 5230 Odense M, Denmark
    Mol Microbiol 40:156-68. 2001
    ..The contact-dependent C-signal transmission mechanism allows the spatial co-ordination of aggregation and sporulation by coupling cell position and signalling levels...
  42. pmc A channel connecting the mother cell and forespore during bacterial endospore formation
    Jeffrey Meisner
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 105:15100-5. 2008
    ..These and other results lead us to suggest that SpoIIIAH forms part of a channel between the forespore and mother cell that is required for the activation of sigma(G)...
  43. pmc Fruiting body formation by Bacillus subtilis
    S S Branda
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 98:11621-6. 2001
    ..subtilis. Other microbial differentiation processes long thought to involve only single cells could display the spatial organization characteristic of multicellular organisms when studied with recent natural isolates...
  44. pmc The peptidoglycan sacculus of Myxococcus xanthus has unusual structural features and is degraded during glycerol-induced myxospore development
    Nhat Khai Bui
    Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Bacteriol 191:494-505. 2009
    ..The spherical, bag-shaped coats isolated from glycerol-induced spores contained no detectable muropeptides, but they contained small amounts of N-acetylmuramic acid and meso-diaminopimelic acid...
  45. pmc CD14-Mac-1 interactions in Bacillus anthracis spore internalization by macrophages
    Claudia Oliva
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294 2170, USA
    Proc Natl Acad Sci U S A 106:13957-62. 2009
    ..Our results show that the binding and uptake of B. anthracis spores by phagocytic cells is a dynamic process and involves multiple receptors and signaling pathways...
  46. pmc Isolation and characterization of superdormant spores of Bacillus species
    Sonali Ghosh
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    J Bacteriol 191:1787-97. 2009
    ..A second important factor may be heat activation of spore populations, since yields of superdormant spores from non-heat-activated spore populations were higher than those from optimally activated spores...
  47. pmc In vivo demonstration and quantification of intracellular Bacillus anthracis in lung epithelial cells
    Brooke H Russell
    Center for Extracellular Matrix Biology, Institute of Biosciences and Technology, Texas A and M Health Science Center, Houston, Texas 77030, USA
    Infect Immun 76:3975-83. 2008
    ..These data, combined with those in our previous reports, provided powerful evidence that the lung epithelia were directly targeted by B. anthracis spores at early stages of infection...
  48. pmc The integrin Mac-1 (CR3) mediates internalization and directs Bacillus anthracis spores into professional phagocytes
    Claudia R Oliva
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294 2170, USA
    Proc Natl Acad Sci U S A 105:1261-6. 2008
    ..anthracis pathogenesis by promoting spore uptake by professional phagocytes and subsequent access to a favorable niche for transport, germination, and outgrowth in lymphoid tissues...
  49. ncbi Cutting Edge: IFN-gamma-producing CD4 T lymphocytes mediate spore-induced immunity to capsulated Bacillus anthracis
    Ian Justin Glomski
    Unité des Toxines et Pathogénie Bactérienne, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Immunol 178:2646-50. 2007
    ..These results are the first evidence of protective cellular immunity against capsulated B. anthracis and suggest that future antianthrax vaccines should strive to augment cellular adaptive immunity...
  50. pmc Functional analysis of Bacillus anthracis protective antigen by using neutralizing monoclonal antibodies
    Fabien Brossier
    Unité Toxines et Pathogénie Bactériennes, URA 2172, CNRS, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    Infect Immun 72:6313-7. 2004
    ..This work contributes to the functional analysis of PA and offers immunotherapeutic perspectives for the treatment of anthrax disease...
  51. pmc Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum
    Keith V Alsaker
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, USA
    J Bacteriol 187:7103-18. 2005
    ....
  52. pmc Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate
    Katerina Ragkousi
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 185:2315-29. 2003
    ..However, other than lacking CwlJ, ywdL spores have no obvious defect in their spore coat. Because of the role for YwdL in a part of the spore germination process, we propose renaming ywdL as a spore germination gene, gerQ...
  53. ncbi Spore germination
    A Moir
    Department of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom
    Cell Mol Life Sci 59:403-9. 2002
    ....
  54. ncbi The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT
    Chetan Bettegowda
    The Howard Hughes Medical Institute, The Ludwig Center for Cancer Genetics and Therapeutics, The Sidney Kimmel Comprehensive Cancer Center, Department of Pharmacology and Molecular Sciences, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Nat Biotechnol 24:1573-80. 2006
    ..Through this analysis, we found that C. novyi-NT spores contained mRNA and that the spore transcripts were distinct from those in vegetative forms of the bacterium...
  55. pmc Bacillus subtilis spore coat
    A Driks
    Department of Microbiology and Immunology, Loyola University Medical Center, Maywood, Illinois 60153
    Microbiol Mol Biol Rev 63:1-20. 1999
    ..We also discuss the importance of posttranslational processing of coat proteins in coat morphogenesis. Finally, we review some of the major outstanding questions in the field...
  56. ncbi I will survive: DNA protection in bacterial spores
    Peter Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Trends Microbiol 15:172-80. 2007
    ..The structure of both DNA and SASP alters upon their association and this causes major changes in the chemical and photochemical reactivity of DNA...
  57. ncbi The forespore line of gene expression in Bacillus subtilis
    Stephanie T Wang
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    J Mol Biol 358:16-37. 2006
    ..Extending earlier work on gene expression in the predivisional cell and the mother cell, we present an integrated overview of the entire program of sporulation gene expression...
  58. ncbi Strategy to inactivate Clostridium perfringens spores in meat products
    Saeed Akhtar
    Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
    Food Microbiol 26:272-7. 2009
    ..Collectively, this study demonstrates the feasibility of an alternative and novel strategy to inactivate C. perfringens spores in meat products formulated with germinants specific for C. perfringens...
  59. ncbi Oral administration of a Bacillus subtilis spore-based vaccine expressing Clonorchis sinensis tegumental protein 22.3 kDa confers protection against Clonorchis sinensis
    Zhenwen Zhou
    Guangzhou Women and Children s Medical Center, 318 Renminzhong road, Guangzhou 510120, People s Republic of China
    Vaccine 26:1817-25. 2008
    ..05) in rats challenged with C. sinensis metacercariae. This report shows that C. sinensis TP22.3 expressed on B. subtilis spores was immunogenic and oral administration of TP22.3 spore can provide protection against C. sinensis...
  60. pmc Compartmentalization of gene expression during Bacillus subtilis spore formation
    David W Hilbert
    Department of Microbiology and Immunology, Temple University School of Medicine, 3400 N Broad St, Philadelphia, PA 19140, USA
    Microbiol Mol Biol Rev 68:234-62. 2004
    ..Activation requires removal of the prosequence following a sigma(G)-directed signal from the prespore...
  61. pmc Examination of potential mechanisms to explain the association between proton pump inhibitors and Clostridium difficile infection
    Michelle M Nerandzic
    Research Service, Louis Stokes Cleveland Veterans Affairs Medical Center, Cleveland, OH 44106, USA
    Antimicrob Agents Chemother 53:4133-7. 2009
    ..Our results suggest that the effects of PPIs in the stomach do not contribute to the pathogenesis of CDI...
  62. pmc Morphogenesis of bacillus spore surfaces
    Venkata G R Chada
    Department of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, Chicago, Illinois 60616, USA
    J Bacteriol 185:6255-61. 2003
    ..Taken together, these results are consistent with the view that the coat is not inert. Rather, the coat is a dynamic structure that accommodates changes in spore volume...
  63. pmc DNA array-based transcriptional analysis of asporogenous, nonsolventogenic Clostridium acetobutylicum strains SKO1 and M5
    Christopher A Tomas
    Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208, USA
    J Bacteriol 185:4539-47. 2003
    ..Several of these expression patterns are new or different from what is known in B. subtilis and can be used to test a number of functional-genomic hypotheses...
  64. pmc Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis
    Cliff S Han
    Department of Energy Joint Genome Institute, Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    J Bacteriol 188:3382-90. 2006
    ..cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms...
  65. ncbi Genetic analysis of the Bacillus subtilis sigG promoter, which controls the sporulation-specific transcription factor sigma G
    Louise Evans
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Microbiology 150:2277-87. 2004
    ..Replacement of the wild-type promoter with sigma(E)-independent promoters resulted in impairment of sporulation. These data support the idea that sigma(E) activity is required for the transcription of sigG...
  66. pmc Cooperativity between different nutrient receptors in germination of spores of Bacillus subtilis and reduction of this cooperativity by alterations in the GerB receptor
    Swaroopa Atluri
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 188:28-36. 2006
    ..vii) The amino acid binding sites in GerA and GerB are different, even though both respond to L-alanine. These new conclusions are integrated into models for the signal transduction pathways that initiate spore germination...
  67. pmc Role of the gerI operon of Bacillus cereus 569 in the response of spores to germinants
    M O Clements
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    J Bacteriol 180:6729-35. 1998
    ..The GerIA protein is predicted to possess an unusually long, charged, N-terminal domain containing nine tandem copies of a 13-amino-acid glutamine- and serine-rich sequence...
  68. pmc An in vivo membrane fusion assay implicates SpoIIIE in the final stages of engulfment during Bacillus subtilis sporulation
    M D Sharp
    Department of Biology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0349, USA
    Proc Natl Acad Sci U S A 96:14553-8. 1999
    ..We suggest that SpoIIIE is required for the final steps of engulfment and that it may regulate or catalyze membrane fusion events...
  69. ncbi Germination of Bacillus cereus spores in response to L-alanine and to inosine: the roles of gerL and gerQ operons
    Paul J Barlass
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Microbiology 148:2089-95. 2002
    ..Although near-identical homologues of gerI and gerL operons are evident in the Bacillus anthracis genome sequence, there is no evidence of a close homologue of gerQ...
  70. pmc A unique GTP-dependent sporulation sensor histidine kinase in Bacillus anthracis
    Francesca Scaramozzino
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Bacteriol 191:687-92. 2009
    ....
  71. ncbi The role of antibodies to Bacillus anthracis and anthrax toxin components in inhibiting the early stages of infection by anthrax spores
    S Welkos
    Divisions of Bacteriology and Pathology, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702 5011, USA
    Microbiology 147:1677-85. 2001
    ..Thus, the anti-PA Ab-specific immunity induced by AVA has anti-spore activity and might have a role in impeding the early stages of infection with B. anthracis spores...
  72. ncbi Thinking about bacterial populations as multicellular organisms
    J A Shapiro
    Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA
    Annu Rev Microbiol 52:81-104. 1998
    ....
  73. ncbi Analysis of factors that influence the sensitivity of spores of Bacillus subtilis to DNA damaging chemicals
    D E Cortezzo
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Appl Microbiol 98:606-17. 2005
    ..To elucidate factors influencing the sensitivity of Bacillus subtilis spores to DNA damaging chemicals...
  74. pmc Identification and characterization of a new prespore-specific regulatory gene, rsfA, of Bacillus subtilis
    L Juan Wu
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    J Bacteriol 182:418-24. 2000
    ..Null mutations in rsfA have different effects on the expression of sigma(F)-dependent genes, suggesting that the RsfA protein is a regulator of transcription that fine-tunes gene expression in the prespore...
  75. ncbi Imaging and 3D elemental characterization of intact bacterial spores by high-resolution secondary ion mass spectrometry
    Sutapa Ghosal
    Lawrence Livermore National Laboratory, Livermore, California 94551, USA
    Anal Chem 80:5986-92. 2008
    ..We use the technique to map the 3D elemental distribution present within Bacillus thuringiensis israelensis spores...
  76. pmc Proteomic profiling and identification of immunodominant spore antigens of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis
    Vito G DelVecchio
    Vital Probes, Inc, 1300 Old Plank Road, Mayfield, PA 18433, USA
    Appl Environ Microbiol 72:6355-63. 2006
    ..anthracis. Comparative analysis of the spore immunome will be of significance for further nucleic acid- and immuno-based detection systems as well as next-generation vaccine development...
  77. pmc FAST CARS: engineering a laser spectroscopic technique for rapid identification of bacterial spores
    M O Scully
    Institute for Quantum Studies, Departments of Physics, Electrical Engineering, and Mechanical Engineering, Texas A and M University, College Station, TX 77843, USA
    Proc Natl Acad Sci U S A 99:10994-1001. 2002
    Airborne contaminants, e.g., bacterial spores, are usually analyzed by time-consuming microscopic, chemical, and biological assays. Current research into real-time laser spectroscopic detectors of such contaminants is based on e.g...
  78. ncbi Mucosal delivery of antigens using adsorption to bacterial spores
    Jen Min Huang
    School of Biological Sciences, Royal Holloway, University of London, Surrey, UK
    Vaccine 28:1021-30. 2010
    ..Of these, the use of genetically engineered bacterial spores has been shown to offer promise as both a mucosal as well as a heat-stable vaccine delivery system...
  79. ncbi Encapsulation of bacterial spores in nanoorganized polyelectrolyte shells
    Shantanu S Balkundi
    Institute for Micromanufacturing and Biomedical Engineering Program, Louisiana Tech University, Ruston, Louisiana, USA
    Langmuir 25:14011-6. 2009
    ..b>Bacterial spores were encapsulated in organized ultrathin shells using layer-by-layer assembly in order to assess the ..
  80. ncbi Phosphatases modulate the bistable sporulation gene expression pattern in Bacillus subtilis
    Jan Willem Veening
    Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    Mol Microbiol 56:1481-94. 2005
    ..This shows that B. subtilis employs multiple pathways to maintain the bistable nature of a sporulating culture, stressing the physiological importance of this phenomenon...
  81. ncbi Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy
    J W Chan
    Physics and Advanced Technologies Directorate, Chemistry and Materials Science Directorate, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
    Anal Chem 76:599-603. 2004
    ..In this work, an inverted confocal Raman microscope is combined with optical trapping to probe and analyze bacterial spores in solution...
  82. pmc Sporulation-specific sigma factor sigma 29 of Bacillus subtilis is synthesized from a precursor protein, P31
    T L LaBell
    Proc Natl Acad Sci U S A 84:1784-8. 1987
    ..Protease-treated P31*, but not untreated P31*, was capable of directing B. subtilis core RNA polymerase to specifically initiate RNA synthesis at a sigma 29-recognized promoter in vitro...
  83. ncbi Analysis of single bacterial spores by micro-Raman spectroscopy
    Anthony P Esposito
    Chemistry and Materials Science, Lawrence Livermore National Laboratory, 7000 East Ave, Livermore, California 94550, USA
    Appl Spectrosc 57:868-71. 2003
  84. pmc Peptide ligands that bind selectively to spores of Bacillus subtilis and closely related species
    Jordan Knurr
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Appl Environ Microbiol 69:6841-7. 2003
    As part of an effort to develop detectors for selected species of bacterial spores, we screened phage display peptide libraries for 7- and 12-mer peptides that bind tightly to spores of Bacillus subtilis...
  85. pmc The physical state of water in bacterial spores
    Erik P Sunde
    Department of Biophysical Chemistry, Center for Molecular Protein Science, Lund University, SE 22100 Lund, Sweden
    Proc Natl Acad Sci U S A 106:19334-9. 2009
    ..The quantitative results reported here on water mobility and transport provide important clues about the mechanism of spore dormancy and resistance, with relevance to food preservation, disease prevention, and astrobiology...
  86. ncbi Mycobacterium versus Streptomyces--we are different, we are the same
    Nicole Scherr
    Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    Curr Opin Microbiol 12:699-707. 2009
    ....
  87. pmc Genomewide insertional mutagenesis in Streptomyces coelicolor reveals additional genes involved in morphological differentiation
    A M Gehring
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 97:9642-7. 2000
    ..Individual members of the three families often are found grouped together in a characteristic arrangement, suggesting that they have a common function...
  88. ncbi Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase
    K T Min
    Department of Biochemistry, University of Oxford, England
    Cell 74:735-42. 1993
    ..We suggest that this phosphorylation is responsible for the compartment-specific release of sigma F activity, perhaps through the formation of a tight complex between SpoIIAB and phosphorylated SpoIIAA...
  89. ncbi A computational analysis of the impact of the transient genetic imbalance on compartmentalized gene expression during sporulation in Bacillus subtilis
    Dagmar Iber
    Mathematical Institute, Centre for Mathematical Biology, University of Oxford, Oxford, UK
    J Mol Biol 360:15-20. 2006
    ..We conclude that the volume difference between the two compartments is the main determinant of cell fate...
  90. ncbi Protection of bacterial spores in space, a contribution to the discussion on Panspermia
    G Horneck
    German Aerospace Center DLR, Institute of Aerospace Medicine, D 51170 Köln, Germany
    Orig Life Evol Biosph 31:527-47. 2001
    ..transfer of life, small rock ejecta of a few cm in diameter could be sufficiently large to protect bacterial spores against the intense insolation; however, micron-sized grains, as originally requested by Panspermia, may not ..
  91. ncbi Regulatory studies on the promoter for a gene governing synthesis and assembly of the spore coat in Bacillus subtilis
    S Cutting
    Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138
    J Mol Biol 207:393-404. 1989
    ..gerE and cotA may be members of a developmental regulon of genes whose products are involved in the assembly of the spore coat...
  92. ncbi Flash detection/identification of pathogens, bacterial spores and bioterrorism agent biomarkers from clinical and environmental matrices
    David C White
    Center for Environmental Biotechnology, University of Tennessee, 10515 Research Drive, Suite 300, Knoxville, TN 37932 2575, USA
    J Microbiol Methods 48:139-47. 2002
    ..The extraction should take about an hour for each sample but multiple samples can be processed simultaneously...
  93. pmc Inactivation of Bacillus anthracis spores
    Ellen A Spotts Whitney
    Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Emerg Infect Dis 9:623-7. 2003
    ..We conducted a survey of the literature that had potential application to the inactivation of B. anthracis spores. This article provides a tabular summary of the results...
  94. pmc Amino acid- and purine ribonucleoside-induced germination of Bacillus anthracis DeltaSterne endospores: gerS mediates responses to aromatic ring structures
    John A W Ireland
    Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Bacteriol 184:1296-303. 2002
    ..We conclude that efficient germination of B. anthracis endospores requires multipartite signals and that gerS-encoded proteins act as an aromatic-responsive germination receptor...
  95. ncbi Microfluidic sonicator for real-time disruption of eukaryotic cells and bacterial spores for DNA analysis
    Theodore Cosmo Marentis
    Harvard Medical School, Boston, MA 02115, USA
    Ultrasound Med Biol 31:1265-77. 2005
    ..Eukaryotic human leukemia HL-60 cells and Bacillus subtilis bacterial spores were lysed as they passed through a microfluidic channel at 50 microL/min and 5 microL/min, respectively, in ..
  96. pmc Human monoclonal antibody AVP-21D9 to protective antigen reduces dissemination of the Bacillus anthracis Ames strain from the lungs in a rabbit model
    Johnny W Peterson
    Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1070, USA
    Infect Immun 75:3414-24. 2007
    ..Early treatment increased the efficacy progressively and in a dose-dependent manner. Thus, AVP-21D9 could offer an adjunct or alternative clinical treatment regimen against inhalation anthrax...
  97. pmc Identification of a gene, spoIIR, that links the activation of sigma E to the transcriptional activity of sigma F during sporulation in Bacillus subtilis
    M L Karow
    Department of Microbiology and Immunology, Temple University Medical School, Philadelphia, PA 19140
    Proc Natl Acad Sci U S A 92:2012-6. 1995
    ..Thus, SpoIIR is required for linking the activation of sigma E to the activation of sigma F and coordinating the initiation of the two developmental programs required to form a spore...
  98. pmc Enhanced detection of surface-associated bacteria in indoor environments by quantitative PCR
    M P Buttner
    Harry Reid Center for Environmental Studies, University of Nevada, Las Vegas, Las Vegas, Nevada 89154-4009, USA
    Appl Environ Microbiol 67:2564-70. 2001
    ....
  99. ncbi Discovery of phage display peptide ligands for species-specific detection of Bacillus spores
    Charles L Turnbough
    Department of Microbiology, University of Alabama at Birmingham, 409 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
    J Microbiol Methods 53:263-71. 2003
    ..This review also discusses other points of interest regarding the use of peptide ligands for spore detection and for the detection of other types of cells...
  100. pmc Environmental sampling for spores of Bacillus anthracis
    Eyasu H Teshale
    Centers for Desease Control and Prevention, Atlanta, Georgia 30333, USA
    Emerg Infect Dis 8:1083-7. 2002
    ..These results suggest cross-contaminated mail as a possible source of anthrax for the inhalational anthrax patient in Connecticut. In future such investigations, extensive sampling guided by epidemiologic data is imperative...
  101. ncbi Macrophage interactions
    C Guidi-Rontani
    Unité Toxines et Pathogénie Bactériennes, CNRS URA 2172, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France
    Curr Top Microbiol Immunol 271:115-41. 2002
    ..The understanding of the complex network of such interactions is likely to allow new therapeutic and preventative strategies to be developed...

Research Grants63

  1. New Method for Detecting Bacillus Anthracis Spores
    Mark Klempner; Fiscal Year: 2003
    ..anthracis spores and spores of other related and unrelated bacteria. The project will focus on developing the ligand library to include non-antibody reagents and differential recognition of bacterial spores from multiple species.
  2. Regulation of pluripotent differentiation by gene circuit interactions.
    GUROL MEHMET SUEL; Fiscal Year: 2013
    ..3) Developing new techniques to control how cells differentiate, which can be applied in the future to control differentiation of mammalian stem cell to substitute for any missing or diseased cell types. ..
  3. Ultrasensitive Detection of Hepatitis Viruses by Immunoassay Amplification
    Boris Rotman; Fiscal Year: 2013
    ..BCR technology exploits phenotypically engineered fluorogenic bacterial spores (F-spores(tm)) as nanodetectors...
  4. Phase I study of Clostridium novyi-NT spores in solid tumor cancer patients
    Luis A Diaz; Fiscal Year: 2010
    ..in this application will evaluate a novel therapeutic strategy in patients with solid tumor malignancies and provide a detailed understanding of the safety, pharmacology and efficacy of live anaerobic bacterial spores in cancer patients.
  5. Mechanism of spore germination
    Philip C Hanna; Fiscal Year: 2012
    ....
  6. Development of Therapeutic Inhibitors to Anthrax Toxins
    JOHNNY PETERSON; Fiscal Year: 2006
    ..Anthrax is a highly infectious disease caused by Bacillus anthracis, and aerosolization of the dried bacterial spores is a major biological warfare and bioterrorism threat...
  7. Dual Antigen Nanoparticle Vaccine System For Bacillus Infectious Disease
    Puthupparampil Scaria; Fiscal Year: 2009
    ..from challenge with Anthrax toxin and, if successful, investigate protection from inhalation exposure to bacterial spores. With success, Phase II studies will be needed to address manufacturing, and preclinical pharmacology and ..
  8. Nisin as a decontaminant for B. anthracis spores on human skin
    Kenneth Alibek; Fiscal Year: 2007
    ..commonly used as a food preservative and has the unique capacity to prevent the germination and outgrowth of bacterial spores. Nisin binds to the surface of the spores and terminates the germination process leaving the spores inert and ..
  9. NEW TREATMENT FOR AMOEBIC KERATITIS
    CLARENCE VILLEMEZ; Fiscal Year: 2001
    ..The therapeutic testing will employ the excellent animal models of acanthamoebic keratitis at the University of Texas Southwestern Medical Center, Dallas and the in vivo experiments will be done there. ..
  10. Enhanced Disinfection of Medical Facilities
    DANIEL AVERY; Fiscal Year: 2003
    ..Phase I will focus on bacterial spores and mycobacterium...
  11. AFM Sensors to Detect Biological Warfare Agents
    Curtis Mosher; Fiscal Year: 2002
    ..Binding of bacterial spores or viral particles to antibodies in defined spatial domains on the chip are detected by direct observation, ..
  12. Generation and Repair of an Unusual UV Photoproduct
    Joan Broderick; Fiscal Year: 2006
    ..becomes the major UV photoproduct under certain conditions, however, including the conditions that exist in bacterial spores (thus the common name is spore photoproduct, SP)...
  13. A New Method for Sterilizing Biomedical Materials
    PENNY STARKEY; Fiscal Year: 2002
    ..Specific aims include (1) to achieve the inactivation of bacterial spores by developing certain process modifications to improve the overall methodology; (2) to conduct a screening ..
  14. A novel platform to discover biodefense therapeutics
    JOHN TRAWICK; Fiscal Year: 2003
    ..United States is particularly alarming considering the relatively primitive methods used to distribute the bacterial spores and the fact that ordinary, antibiotic-sensitive strains of the anthrax bacillus were used in the attacks...
  15. COLD STERILANT FOR DELICATE MEDICAL INSTRUMENTS
    Jack Kessler; Fiscal Year: 1992
    ..We are developing a cold sterilization technology that is rapidly biocidal against fungi, viruses, bacteria and bacterial spores, but is neither toxic nor mutagenic to higher organisms...
  16. Sterilization of Mail using Variable Frequency Microwave
    ZAKARYAE FATHI; Fiscal Year: 2002
    ..These studies will be validated with additional species including live veterinary vaccine strains of B. anthracis (Sterne 34F2 for example) as part of Phase II process studies. ..
  17. Destruction of Air-Borne Pathogenic Bacteria
    JAMES KITTRELL; Fiscal Year: 2002
    ..technology to inactivate air-borne Bacillus anthracis (anthrax), using representative surrogate bacterial spores, B. cereus...
  18. Analyzer for 2D Multiplex Detection of Infectious Agents
    Richard Mathies; Fiscal Year: 2007
    ..6) The optimized portable systems will be used to perform analyses of more pathogenic bacteria, viruses and bacterial spores. (7) In addition, a high-throughput, parallel 96 sample version of the immuno-genetic analyzer will be ..
  19. ELEVENTH INTERNATIONAL SPORES CONFERENCE
    PATRICK PIGGOT; Fiscal Year: 1992
    ..May 9-13, 1992. The extraordinary resistance of bacterial spores, the diversity of sporeforming species, and the importance (good and bad) of sporeformers to medicine, to ..
  20. Septum Asymmetry as a Cell Fate Determinant
    Eric Becker; Fiscal Year: 2004
    ..b>Bacterial spores are highly resistant to many common sterilization techniques and often are implicated in food-borne illness...
  21. REGULATION OF BACTERIAL SPORE DEVELOPMENT
    ROBERT KADNER; Fiscal Year: 1980
    ..The organization and expression of the rRNA cistrons will be compared to the situation in E. coli. The experiments proposed here will allow determination of the sequence of the stable RNAs in this organism...
  22. Protein Aspartate Phosphatases in Development
    Marta Perego; Fiscal Year: 2006
    ..The role of the Rap phosphatases not involved in sporulation will be investigated by microbiological and genetic approaches ..
  23. ASSEMBLY OF THE SPORE COAT OF B SUBTILIS
    Adam Driks; Fiscal Year: 2005
    b>Bacterial spores are among the most extraordinary cell types found in nature...
  24. Signal Transduction in Enterococci
    Marta Perego; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  25. IDENTIFICATION OF B. ANTRACIS SPORE-SURFACE PROTEINS
    Adam Driks; Fiscal Year: 2003
    ..subtilis coats ongoing in our laboratory, as well as the available genome sequences of the two organisms. Ultimately, this work will enable development of a new class of small, robust versatile B. anthracis spore detectors. ..
  26. PEPTIDOGLYCAN SYNTHESIS IN BACILLUS SUBTILIS
    DAVID POPHAM; Fiscal Year: 2008
    ..abstract_text> ..
  27. STATISTICAL METHODS IN AIDS RESEARCH
    Ronald Brookmeyer; Fiscal Year: 2001
    ..The methods will be applied to the ALIVE study. ..
  28. SPORE PEPTIDOGLYCAN DEGRADATION IN BACILLUS ANTHRACIS
    DAVID POPHAM; Fiscal Year: 2005
    ....
  29. Statistical Models for Anthrax
    Ronald Brookmeyer; Fiscal Year: 2003
    ..This work will depend on results from specific aims 1 and 2. ..
  30. STRUCTURAL STUDIES OF BACTERIAL HYALURONATE LYASE ENZYME
    MARK JEDRZEJAS; Fiscal Year: 2003
    ..The results will enhance our knowledge about mechanisms involved in the action of the enzyme and in the pathogenesis of S. pneumoniae bacteria. ..
  31. Clostridium difficile Toxin Gene Regulation
    ABRAHAM LINCOLN SONENSHEIN; Fiscal Year: 2010
    ..abstract_text> ..
  32. Meeting on the Pathogenesis of Clostridia
    ABRAHAM SONENSHEIN; Fiscal Year: 2003
    ..The conference will conclude with an in-depth analysis of interactions between the pathogenic clostridia and their hosts in Session 10. ..
  33. Mucin-degrading microflora for prophylactic antibiotics
    ROBERT CARMAN; Fiscal Year: 2008
    ..difficile, and reduces the incidence of C. difficile- induced antibiotic-associated diarrhea. [unreadable] [unreadable] [unreadable]..
  34. Prevention of Clostridium difficile-associated disease
    MYRON SASSER; Fiscal Year: 2008
    ..This SBIR research proposes prevention of CDAD through the use of adjuvants with the antibiotics. [unreadable] [unreadable] [unreadable]..
  35. The role of C1q in regulation of monocyte to dendritic cell transition
    Berhane Ghebrehiwet; Fiscal Year: 2010
    ..G w1' 0.N ..r v0, .a. ' in the long term, may provide firm grounds for the development of innovative therapeutic intervention strategies. ..O (.) '-" .+ 4U 4.' .U- 0_0 ..
  36. gC1q-R/p33: structure function and role on inflammation.
    Berhane Ghebrehiwet; Fiscal Year: 2009
    ..abstract_text> ..
  37. Label-Free High-Throughput Screening Platform
    ANN GROW; Fiscal Year: 2006
    ..The platform will be simple to use, very low-cost, and produce a rich body of information on the binding interactions. [unreadable] [unreadable]..
  38. Rapid Susceptibility Testing of MDR M. tuberculosis- Phase II
    MYRON SASSER; Fiscal Year: 2007
    ..Although not crucial to the success of the project, direct-from-sputum analysis for MTB identification and susceptibility testing will also be evaluated. [unreadable] [unreadable] [unreadable]..
  39. Oxidative Stress in Oral Streptococci
    Robert E Marquis; Fiscal Year: 2010
    ..The work involves basic and applied studies Aimed at a better understanding of the biology of Dental plaque bacteria related especially to oxygen metabolism and damage caused by reactive oxygen species. ..
  40. mazEF-mediated programmed cell death network in E. coli
    Hanna Engelberg Kulka; Fiscal Year: 2010
    ..Practically, the results of our study may be useful as a basis for a novel strategy for generating a new class of antibiotics that trigger bacterial PCD, and thereby to help what has become a public health problem. ..
  41. Targeting protein synthesis in AML
    ARTHUR FRANKEL; Fiscal Year: 2006
    ..These studies will hopefully lead to the design of pivotal phase II and III clinical trials which will define the role of DT388IL3 in the management of AML. ..
  42. BACTERIAL CELL DIVISION
    Lawrence Rothfield; Fiscal Year: 2002
    ..4. To determine whether the residual division sites that are present at the cell poles play a role in the generation of future division sites within the cell. ..
  43. HOST DETERMINANTS OF INFECTIOUSNESS IN TUBERCULOSIS
    Kevin Fennelly; Fiscal Year: 2003
    ..This approach may open a new field of investigation of the host determinants of infectiousness, which could be extended to other respiratory infectious agents in the future. ..
  44. HOST-PATHOGEN GENE EXPRESSION DURING A PULMONARY MYCOBAC
    CLIFFORD LYONS; Fiscal Year: 2003
    ..abstract_text> ..
  45. Phospholipid-induced Motility in Bacillus cereus Group
    R Fall; Fiscal Year: 2004
    ..Such information will be thebasis for future studies or the biochemical mechanism of gliding motility in B. cereus family, and may lead to new strategies to control tissue invasion by these pathogens. ..
  46. REAL TIME DETECTION OF BACTERIA IN PLATELET CONCENTRATES
    Boris Rotman; Fiscal Year: 2004
    ..Other potential applications of the bacteriologic biosensor include: diagnostics, environmental surveillance, food and beverage monitoring, and sterility testing. ..
  47. Identification of E. histolytica virulence factors
    Molly Hughes; Fiscal Year: 2004
    ..Successful completion of these studies will yield a fundamental understanding of amebic proteins that interact with the E. histolytica GalNAc lectin and their role in pathogenicity. ..
  48. C. difficile Toxin Membrane Test with Magnetic Particles
    ROBERT CARMAN; Fiscal Year: 2004
    ..perfringens enterotoxin, another cause of AAD. The technology developed in this project will be widely applicable for the development of new highly sensitive stool antigen tests. ..
  49. Mining the Soil Metagenome for Novel Biodegradation Gen*
    Ronald Crawford; Fiscal Year: 2004
    ..abstract_text> ..
  50. Studies on Macrophage Resistance to Anthrax Lethal Toxin
    Molly Hughes; Fiscal Year: 2004
    ..Successful completion of these studies will yield a fundamental understanding of mechanisms of macrophage resistance to LT. ..
  51. Genomic Comparison of Infectious Strains E. Faecalis
    George Weinstock; Fiscal Year: 2006
    ..The introduction of the novel methods will reduce costs and increase throughput, both of which are important for future scale-up to larger numbers of strains. ..
  52. CHARACTERIZATION OF B ANTHRACIS EXOSPORIUM PROTEINS
    CHARLES TURNBOUGH; Fiscal Year: 2004
    ..anthracis spore surface (i.e., exosporium) to create affinity-matured single chain antibodies that neutralize exosporium protein activity, then test the effects of these reagents on spore properties. ..
  53. Diphtheria fusion protein therapy of AML
    ARTHUR FRANKEL; Fiscal Year: 2006
    ..These studies should lead to the design of pivotal phase II clinical trials to determine the role of this therapeutic in AML management. ..
  54. Non-Enzymatic Cell Lysis/Protein Digestion Proteomics
    Franco Basile; Fiscal Year: 2005
    ..Advantages anticipated from these studies include the ability to perform high throughput sample preparation for proteomic measurements and its direct effect on the rapid diagnosis of microbial related diseases. ..
  55. ASERS -Label-Free Assay for AIDS Drug Development
    ANN GROW; Fiscal Year: 2005
    ..g., binding interactions with biomolecule complexes, competitive, synergistic, or antagonistic binding, and/or the impact of pH or metal ions on ligand binding interactions. ..
  56. Francisella Genomics
    George Weinstock; Fiscal Year: 2004
    ..tularensis contains a high proportion of unique genes compared to other bacterial genomes. Therefore, knowledge of the complete genome sequences of F. tularensis strains is needed for the understanding of the virulence mechanisms. ..
  57. REGULATION OF PYRIMIDINE GENE EXPRESSION IN BACTERIA
    CHARLES TURNBOUGH; Fiscal Year: 2001
    ..Although these studies are done with E. coli, the work is likely to be applicable to the study of gene expression and regulation in all bacteria and probably in eukaryotes, as well. ..
  58. STRATEGIES FOR INACTIVATING BACILLUS ANTHRACIS SPORES
    Arthur Aronson; Fiscal Year: 2003
    ..Once such reagents or methods have been identified, they will be tested on spores in various soils that should mimic natural environments and thus the conditions needed for the effective control of this bioweapon. ..
  59. Combination fusion toxin and cytarabine therapy of AML
    ARTHUR FRANKEL; Fiscal Year: 2002
    ..We anticipate these studies should provide the basis for the design and implementation of pivotal multi-institutional phase II and III trials of DTGM/cytarabine therapy for relapsed/refractory AML. ..