pseudomonas aeruginosa

Summary

Summary: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.

Top Publications

  1. pmc Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients
    Eric E Smith
    Genome Center, Program in Molecular and Cellular Biology, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 103:8487-92. 2006
  2. pmc A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria
    Rachel D Hood
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Cell Host Microbe 7:25-37. 2010
  3. pmc Type VI secretion delivers bacteriolytic effectors to target cells
    Alistair B Russell
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    Nature 475:343-7. 2011
  4. ncbi Mechanisms of biofilm resistance to antimicrobial agents
    T F Mah
    Dept of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
    Trends Microbiol 9:34-9. 2001
  5. ncbi Extracellular DNA required for bacterial biofilm formation
    Cynthia B Whitchurch
    ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, QLD, Australia
    Science 295:1487. 2002
  6. pmc A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus
    Joseph D Mougous
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 312:1526-30. 2006
  7. ncbi A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms
    Marie Allesen-Holm
    Centre for Biomedical Microbiology, BioCentrum DTU, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Mol Microbiol 59:1114-28. 2006
  8. pmc The type III secretion system of Pseudomonas aeruginosa: infection by injection
    Alan R Hauser
    Departments of MicrobiologyImmunology and Medicine, Northwestern University, Chicago, Illinois 60611, USA
    Nat Rev Microbiol 7:654-65. 2009
  9. pmc The CRISPR/Cas adaptive immune system of Pseudomonas aeruginosa mediates resistance to naturally occurring and engineered phages
    Kyle C Cady
    Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA
    J Bacteriol 194:5728-38. 2012
  10. pmc Dynamics of Pseudomonas aeruginosa genome evolution
    Kalai Mathee
    Department of Molecular Microbiology and Immunology, College of Medicine, School of Computing and Information Sciences, College of Engineering, Florida International University, Miami, FL 33199, USA
    Proc Natl Acad Sci U S A 105:3100-5. 2008

Detail Information

Publications339 found, 100 shown here

  1. pmc Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients
    Eric E Smith
    Genome Center, Program in Molecular and Cellular Biology, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 103:8487-92. 2006
    ..of one such infection, that of a cystic fibrosis (CF) patient by the opportunistic bacterial pathogen Pseudomonas aeruginosa. The bacteria underwent numerous genetic adaptations during 8 years of infection, as evidenced by a ..
  2. pmc A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria
    Rachel D Hood
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Cell Host Microbe 7:25-37. 2010
    ..Here we analyzed the secretomes of Pseudomonas aeruginosa mutants altered in regulation of the Hcp Secretion Island-I-encoded type VI secretion system (H1-T6SS)...
  3. pmc Type VI secretion delivers bacteriolytic effectors to target cells
    Alistair B Russell
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    Nature 475:343-7. 2011
    ..Here we show that the type VI secretion system of Pseudomonas aeruginosa breaches this barrier to deliver two effector proteins, Tse1 and Tse3, to the periplasm of recipient cells...
  4. ncbi Mechanisms of biofilm resistance to antimicrobial agents
    T F Mah
    Dept of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
    Trends Microbiol 9:34-9. 2001
    ..Recent research has begun to shed light on how and why surface-attached microbial communities develop resistance to antimicrobial agents...
  5. ncbi Extracellular DNA required for bacterial biofilm formation
    Cynthia B Whitchurch
    ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, QLD, Australia
    Science 295:1487. 2002
  6. pmc A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus
    Joseph D Mougous
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 312:1526-30. 2006
    ..Here we found that a virulence locus (HSI-I) of Pseudomonas aeruginosa encodes a protein secretion apparatus...
  7. ncbi A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms
    Marie Allesen-Holm
    Centre for Biomedical Microbiology, BioCentrum DTU, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Mol Microbiol 59:1114-28. 2006
    b>Pseudomonas aeruginosa produces extracellular DNA which functions as a cell-to-cell interconnecting matrix component in biofilms...
  8. pmc The type III secretion system of Pseudomonas aeruginosa: infection by injection
    Alan R Hauser
    Departments of MicrobiologyImmunology and Medicine, Northwestern University, Chicago, Illinois 60611, USA
    Nat Rev Microbiol 7:654-65. 2009
    The Gram-negative bacterium Pseudomonas aeruginosa uses a complex type III secretion apparatus to inject effector proteins into host cells...
  9. pmc The CRISPR/Cas adaptive immune system of Pseudomonas aeruginosa mediates resistance to naturally occurring and engineered phages
    Kyle C Cady
    Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA
    J Bacteriol 194:5728-38. 2012
    ..of 6 temperate bacteriophages (phages) that are prevented from replicating within the laboratory strain Pseudomonas aeruginosa PA14 by the endogenous CRISPR/Cas system of this microbe...
  10. pmc Dynamics of Pseudomonas aeruginosa genome evolution
    Kalai Mathee
    Department of Molecular Microbiology and Immunology, College of Medicine, School of Computing and Information Sciences, College of Engineering, Florida International University, Miami, FL 33199, USA
    Proc Natl Acad Sci U S A 105:3100-5. 2008
    One of the hallmarks of the Gram-negative bacterium Pseudomonas aeruginosa is its ability to thrive in diverse environments that includes humans with a variety of debilitating diseases or immune deficiencies...
  11. pmc Pseudomonas Genome Database: facilitating user-friendly, comprehensive comparisons of microbial genomes
    Geoffrey L Winsor
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
    Nucleic Acids Res 37:D483-8. 2009
    b>Pseudomonas aeruginosa is a well-studied opportunistic pathogen that is particularly known for its intrinsic antimicrobial resistance, diverse metabolic capacity, and its ability to cause life threatening infections in cystic fibrosis ..
  12. pmc Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms
    Philip D Lister
    Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA
    Clin Microbiol Rev 22:582-610. 2009
    ..This is especially true for infections caused by the opportunistic pathogen Pseudomonas aeruginosa, with its ability to rapidly develop resistance to multiple classes of antibiotics...
  13. pmc The clinical impact of bacterial biofilms
    Niels Høiby
    Department of Clinical Microbiology, Rigshospitalet
    Int J Oral Sci 3:55-65. 2011
    ..Likewise, chronic Pseudomonas aeruginosa lung infections in cystic fibrosis patients are caused by biofilm growing mucoid strains...
  14. pmc The sensor kinase CbrA is a global regulator that modulates metabolism, virulence, and antibiotic resistance in Pseudomonas aeruginosa
    Amy T Y Yeung
    University of British Columbia, Vancouver, BC, Canada
    J Bacteriol 193:918-31. 2011
    b>Pseudomonas aeruginosa is an opportunistic pathogen that possesses a large arsenal of virulence factors enabling the pathogen to cause serious infections in immunocompromised patients, burn victims, and cystic fibrosis patients...
  15. ncbi The small RNA PhrS stimulates synthesis of the Pseudomonas aeruginosa quinolone signal
    Elisabeth Sonnleitner
    Max F Perutz Laboratories, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Dr Bohrgasse 9, 1030 Vienna, Austria
    Mol Microbiol 80:868-85. 2011
    ..a cell-to-cell communication system based on small signal molecules, is employed by the human pathogen Pseudomonas aeruginosa to regulate virulence and biofilm development...
  16. pmc Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes: a transcriptome analysis
    Martin Schuster
    Department of Microbiology and W M Keck Microbial Communities and Cell Signaling Program, University of Iowa, Iowa City, Iowa 52242, USA
    J Bacteriol 185:2066-79. 2003
    There are two interrelated acyl-homoserine lactone quorum-sensing-signaling systems in Pseudomonas aeruginosa. These systems, the LasR-LasI system and the RhlR-RhlI system, are global regulators of gene expression...
  17. ncbi Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons
    Sophie Bleves
    CNRS, Aix Marseille Universite, Institut de Microbiologie de la Mediterranee, France
    Int J Med Microbiol 300:534-43. 2010
    ..The Gram-negative opportunistic human pathogen Pseudomonas aeruginosa possesses a broad panel of secretion systems...
  18. ncbi Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
    C K Stover
    PathoGenesis Corporation, Seattle, Washington 98119, USA
    Nature 406:959-64. 2000
    b>Pseudomonas aeruginosa is a ubiquitous environmental bacterium that is one of the top three causes of opportunistic human infections...
  19. pmc Carbapenemases: the versatile beta-lactamases
    Anne Marie Queenan
    Johnson and Johnson Pharmaceutical Research and Development, L L C, Raritan, NJ 08869, USA
    Clin Microbiol Rev 20:440-58, table of contents. 2007
    ..belong to the IMP, VIM, SPM, GIM, and SIM families and have been detected primarily in Pseudomonas aeruginosa; however, there are increasing numbers of reports worldwide of this group of beta-lactamases in the ..
  20. pmc Self-generated diversity produces "insurance effects" in biofilm communities
    Blaise R Boles
    Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 101:16630-5. 2004
    ..Here we show that the opportunistic pathogen Pseudomonas aeruginosa undergoes extensive genetic diversification during short-term growth in biofilm communities...
  21. pmc Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool Epidemic Strain of Pseudomonas aeruginosa
    Craig Winstanley
    Division of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom
    Genome Res 19:12-23. 2009
    b>Pseudomonas aeruginosa isolates have a highly conserved core genome representing up to 90% of the total genomic sequence with additional variable accessory genes, many of which are found in genomic islands or islets...
  22. pmc The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms
    H Ceri
    Biofilm Research Group, University of Calgary, Calgary, Alberta, Canada T2N 1N4
    J Clin Microbiol 37:1771-6. 1999
    ..Committee for Clinical Laboratory Standards (NCCLS) reference strains: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213...
  23. pmc Antibiotics as intermicrobial signaling agents instead of weapons
    J F Linares
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Darwin 3, Campus Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 103:19484-9. 2006
    ..trigger expression of determinants influencing the virulence of the major opportunistic bacterial pathogen Pseudomonas aeruginosa. All three antibiotics induce biofilm formation; tobramycin increases bacterial motility, and tetracycline ..
  24. ncbi Pseudomonas aeruginosa: all roads lead to resistance
    Elena B M Breidenstein
    Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
    Trends Microbiol 19:419-26. 2011
    b>Pseudomonas aeruginosa is often resistant to multiple antibiotics and consequently has joined the ranks of 'superbugs' due to its enormous capacity to engender resistance...
  25. ncbi Pseudomonas aeruginosa - a phenomenon of bacterial resistance
    Tanya Strateva
    Department of Microbiology, Medical University of Sofia, 1431 Sofia, Bulgaria
    J Med Microbiol 58:1133-48. 2009
    b>Pseudomonas aeruginosa is one of the leading nosocomial pathogens worldwide...
  26. ncbi Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants
    Mikkel Klausen
    Molecular Microbial Ecology Group, BioCentrum DTU, Building 301, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Mol Microbiol 48:1511-24. 2003
    Biofilm formation by Gfp-tagged Pseudomonas aeruginosa PAO1 wild type, flagella and type IV pili mutants in flow chambers irrigated with citrate minimal medium was characterized by the use of confocal laser scanning microscopy and comstat ..
  27. pmc Distinct pathogenesis and host responses during infection of C. elegans by P. aeruginosa and S. aureus
    Javier E Irazoqui
    Program of Developmental Immunology, Department of Pediatrics, Massachusetts General Hospital, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 6:e1000982. 2010
    ..b>Pseudomonas aeruginosa and Staphylococcus aureus utilize virulence factors involved in human disease to infect and kill C...
  28. ncbi A conserved p38 MAP kinase pathway in Caenorhabditis elegans innate immunity
    Dennis H Kim
    Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
    Science 297:623-6. 2002
    A genetic screen for Caenorhabditis elegans mutants with enhanced susceptibility to killing by Pseudomonas aeruginosa led to the identification of two genes required for pathogen resistance: sek-1, which encodes a mitogen-activated ..
  29. pmc Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains
    Jens Klockgether
    Klinische Forschergruppe, Medizinische Hochschule Hannover, Hannover, Germany
    J Bacteriol 192:1113-21. 2010
    b>Pseudomonas aeruginosa PAO1 is the most commonly used strain for research on this ubiquitous and metabolically versatile opportunistic pathogen...
  30. ncbi Pseudomonas aeruginosa: a formidable and ever-present adversary
    K G Kerr
    Department of Microbiology, Harrogate District Hospital, Lancaster Park Road, Harrogate, North Yorkshire HG2 7SX, UK
    J Hosp Infect 73:338-44. 2009
    b>Pseudomonas aeruginosa is a versatile pathogen associated with a broad spectrum of infections in humans...
  31. ncbi Genes involved in matrix formation in Pseudomonas aeruginosa PA14 biofilms
    Lisa Friedman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 51:675-90. 2004
    b>Pseudomonas aeruginosa forms diverse matrix-enclosed surface-associated multicellular assemblages (biofilms) that aid in its survival in a variety of environments...
  32. pmc Identification of FleQ from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor
    Jason W Hickman
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Mol Microbiol 69:376-89. 2008
    ..We have identified the protein FleQ as a c-di-GMP-responsive transcriptional regulator in Pseudomonas aeruginosa. FleQ is known to activate expression of flagella biosynthesis genes...
  33. pmc Quorum sensing and virulence of Pseudomonas aeruginosa during lung infection of cystic fibrosis patients
    Thomas Bjarnsholt
    Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 5:e10115. 2010
    b>Pseudomonas aeruginosa is the predominant microorganism in chronic lung infection of cystic fibrosis patients. The chronic lung infection is preceded by intermittent colonization...
  34. ncbi The Pseudomonas aeruginosa sensor RetS switches type III and type VI secretion via c-di-GMP signalling
    Joana A Moscoso
    Imperial College London, Division of Cell and Molecular Biology, Centre for Molecular Microbiology and Infection, South Kensington Campus, Flowers Building, London SW7 2AZ, UK
    Environ Microbiol 13:3128-38. 2011
    ..In Pseudomonas aeruginosa, the transition between motility and sessility involves regulatory networks including the RetS/GacS ..
  35. pmc Pseudomonas aeruginosa RhlR is required to neutralize the cellular immune response in a Drosophila melanogaster oral infection model
    Stefanie Limmer
    Equipe Fondation Recherche Médicale, Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, Universite de Strasbourg, F67084 Strasbourg Cedex, France
    Proc Natl Acad Sci U S A 108:17378-83. 2011
    ..Both Drosophila melanogaster and Pseudomonas aeruginosa have been intensively studied. Here, we analyze the infection of D. melanogaster by P...
  36. pmc Evolutionary dynamics of bacteria in a human host environment
    Lei Yang
    Department of Systems Biology, Technical University of Denmark, 2800 Lyngby, Denmark
    Proc Natl Acad Sci U S A 108:7481-6. 2011
    ..the evolutionary dynamics of a lineage of a clinically important opportunistic bacterial pathogen, Pseudomonas aeruginosa, as it adapts to the airways of several individual cystic fibrosis patients over 200,000 bacterial ..
  37. pmc Endogenous oxidative stress produces diversity and adaptability in biofilm communities
    Blaise R Boles
    Department of Microbiology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 105:12503-8. 2008
    ..Here, we used biofilms formed by the pathogen Pseudomonas aeruginosa to investigate how this population diversity is produced...
  38. ncbi Multidrug efflux pumps and antimicrobial resistance in Pseudomonas aeruginosa and related organisms
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, ON, Canada
    J Mol Microbiol Biotechnol 3:255-64. 2001
    b>Pseudomonas aeruginosa is an opportunistic human pathogen characterized by an innate resistance to multiple antimicrobial agents...
  39. ncbi Quorum sensing and environmental adaptation in Pseudomonas aeruginosa: a tale of regulatory networks and multifunctional signal molecules
    Paul Williams
    Centre for Biomolecular Sciences, University of Nottingham, United Kingdom
    Curr Opin Microbiol 12:182-91. 2009
    ....
  40. pmc Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications
    D W Essar
    Microbiology Department, University of Iowa, Iowa City 52242
    J Bacteriol 172:884-900. 1990
    Two anthranilate synthase gene pairs have been identified in Pseudomonas aeruginosa. They were cloned, sequenced, inactivated in vitro by insertion of an antibiotic resistance gene, and returned to P...
  41. pmc Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins
    Abderrahman Hachani
    Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 286:12317-27. 2011
    b>Pseudomonas aeruginosa is a Gram-negative bacterium causing chronic infections in cystic fibrosis patients...
  42. pmc Immune recognition of Pseudomonas aeruginosa mediated by the IPAF/NLRC4 inflammasome
    Fayyaz S Sutterwala
    Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Exp Med 204:3235-45. 2007
    b>Pseudomonas aeruginosa is a Gram-negative bacterium that causes opportunistic infections in immunocompromised individuals. P. aeruginosa employs a type III secretion system to inject effector molecules into the cytoplasm of the host cell...
  43. ncbi A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO 80523, USA
    J Microbiol Methods 64:391-7. 2006
    A rapid microcentrifuge-based method is described for preparation of Pseudomonas aeruginosa electrocompetent cells with up to 10,000-fold increased transformation efficiencies over existing procedures...
  44. ncbi A signaling network reciprocally regulates genes associated with acute infection and chronic persistence in Pseudomonas aeruginosa
    Andrew L Goodman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 7:745-54. 2004
    The opportunistic pathogen Pseudomonas aeruginosa causes a variety of acute and chronic infections...
  45. ncbi Dietary phytochemicals as quorum sensing inhibitors
    D A Vattem
    Molecular and Cellular Nutrition Laboratory, FCS Department, Texas State University, 601 University Drive, San Marcos, TX 78666, USA
    Fitoterapia 78:302-10. 2007
    ..concentrations of the extracts on swarming motility of pathogens Escherichia coli (EC)O157:H7 and Pseudomonas aeruginosa (PA-01). Our results indicate that all extracts significantly inhibited quorum sensing...
  46. pmc A polymicrobial perspective of pulmonary infections exposes an enigmatic pathogen in cystic fibrosis patients
    Christopher D Sibley
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, AB, Canada T2N 4N1
    Proc Natl Acad Sci U S A 105:15070-5. 2008
    ..If a given organism, such as Pseudomonas aeruginosa, becomes resistant to antibiotic therapy, an alternative treatment avenue may mediate the desired clinical ..
  47. ncbi Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare?
    David M Livermore
    Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, Colindale, London, United Kingdom
    Clin Infect Dis 34:634-40. 2002
    b>Pseudomonas aeruginosa carries multiresistance plasmids less often than does Klebsiella pneumoniae, develops mutational resistance to cephalosporins less readily than Enterobacter species, and has less inherent resistance than ..
  48. pmc Redox-active antibiotics control gene expression and community behavior in divergent bacteria
    Lars E P Dietrich
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 01239, USA
    Science 321:1203-6. 2008
    It is thought that bacteria excrete redox-active pigments as antibiotics to inhibit competitors. In Pseudomonas aeruginosa, the endogenous antibiotic pyocyanin activates SoxR, a transcription factor conserved in Proteo- and Actinobacteria...
  49. pmc The broad host range pathogen Pseudomonas aeruginosa strain PA14 carries two pathogenicity islands harboring plant and animal virulence genes
    Jianxin He
    Department of Surgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 101:2530-5. 2004
    The ubiquitous bacterium Pseudomonas aeruginosa is the quintessential opportunistic pathogen. Certain isolates infect a broad range of host organisms, from plants to humans...
  50. pmc Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1
    D V Mavrodi
    Department of Plant Pathology, Agricultural Research Service, Washington State University, Pullman, Washington 99164 6430, USA
    J Bacteriol 183:6454-65. 2001
    Two seven-gene phenazine biosynthetic loci were cloned from Pseudomonas aeruginosa PAO1...
  51. ncbi Malabaricone C from Myristica cinnamomea exhibits anti-quorum sensing activity
    Yee Meng Chong
    Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
    J Nat Prod 74:2261-4. 2011
    ..Furthermore, 1 inhibited the quorum sensing-regulated pyocyanin production and biofilm formation in Pseudomonas aeruginosa PAO1...
  52. pmc C. elegans detects pathogen-induced translational inhibition to activate immune signaling
    Tiffany L Dunbar
    Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA
    Cell Host Microbe 11:375-86. 2012
    ..Upon infection with pathogenic Pseudomonas aeruginosa, the nematode C...
  53. pmc Pulmonary bacteriophage therapy on Pseudomonas aeruginosa cystic fibrosis strains: first steps towards treatment and prevention
    Eric Morello
    Molecular Biology of the Gene in Extremophiles Unit, Department of Microbiology, Institut Pasteur, Paris, France
    PLoS ONE 6:e16963. 2011
    ..Using a mouse lung-infection model caused by a multidrug resistant Pseudomonas aeruginosa mucoid strain isolated from a cystic fibrosis patient, we evaluated bacteriophage treatments...
  54. ncbi Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA
    Anja Brencic
    Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 72:612-32. 2009
    ..In this study, we identified the regulon of RsmA of the Pseudomonas aeruginosa PAK strain by using cultures in the stationary phase of growth...
  55. pmc Emergence of NDM-1 metallo-β-lactamase in Pseudomonas aeruginosa clinical isolates from Serbia
    Branko Jovcic
    Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
    Antimicrob Agents Chemother 55:3929-31. 2011
    This work reports, for the first time, the presence of New Delhi metallo-β-lactamase 1 (NDM-1) in Pseudomonas aeruginosa. Moreover, this is the first report of the NDM-1 presence in the Balkan region...
  56. ncbi Targeting multiple targets in Pseudomonas aeruginosa PAO1 using flux balance analysis of a reconstructed genome-scale metabolic network
    Deepak Perumal
    Advanced Design and Modeling Lab, Nanyang Technological University, Singapore
    J Drug Target 19:1-13. 2011
    ..Here, we investigate the essentiality of enzymes in iMO1056 metabolic model of nosocomial pathogen Pseudomonas aeruginosa by performing in silico enzyme deletions using FBA...
  57. pmc Host translational inhibition by Pseudomonas aeruginosa Exotoxin A Triggers an immune response in Caenorhabditis elegans
    Deborah L McEwan
    Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Cell Host Microbe 11:364-74. 2012
    ..To understand the mechanisms underlying pathogen recognition, we investigated how Pseudomonas aeruginosa triggers intestinal innate immunity in Caenorhabditis elegans, a process independent of Toll-like pattern ..
  58. pmc Identification of catechin as one of the flavonoids from Combretum albiflorum bark extract that reduces the production of quorum-sensing-controlled virulence factors in Pseudomonas aeruginosa PAO1
    Olivier M Vandeputte
    Plant Biotechnology Unit, BioVallée, 8 rue Adrienne Bolland, B 6041 Gosselies, Belgium
    Appl Environ Microbiol 76:243-53. 2010
    Quorum-sensing (QS) regulates the production of key virulence factors in Pseudomonas aeruginosa and other important pathogenic bacteria. In this report, extracts of leaves and bark of Combretum albiflorum (Tul...
  59. ncbi Key two-component regulatory systems that control biofilm formation in Pseudomonas aeruginosa
    Helga Mikkelsen
    Imperial College London, Division of Cell and Molecular Biology, Centre for Molecular Microbiology and Infection, South Kensington Campus, Flowers Building, London SW7 2AZ, UK
    Environ Microbiol 13:1666-81. 2011
    ....
  60. pmc Pathogenicity islands PAPI-1 and PAPI-2 contribute individually and synergistically to the virulence of Pseudomonas aeruginosa strain PA14
    Ewan M Harrison
    Department of Infection, University of Leicester, Leicester LE1 9HN, United Kingdom
    Infect Immun 78:1437-46. 2010
    b>Pseudomonas aeruginosa is a leading cause of hospital-acquired pneumonia and severe chronic lung infections in cystic fibrosis patients...
  61. ncbi A controlled clinical trial of a therapeutic bacteriophage preparation in chronic otitis due to antibiotic-resistant Pseudomonas aeruginosa; a preliminary report of efficacy
    A Wright
    UCL Ear Institute and Royal National Throat, Nose and Ear Hospital, Grays Inn Road, London, UK
    Clin Otolaryngol 34:349-57. 2009
    To evaluate the efficacy and safety of a therapeutic bacteriophage preparation (Biophage-PA) targeting antibiotic-resistant Pseudomonas aeruginosa in chronic otitis.
  62. pmc The GacS/GacA signal transduction system of Pseudomonas aeruginosa acts exclusively through its control over the transcription of the RsmY and RsmZ regulatory small RNAs
    Anja Brencic
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 73:434-45. 2009
    We report here the results of an analysis of the regulatory range of the GacS/GacA two-component system in Pseudomonas aeruginosa. Using microarrays, we identified a large number of genes that are regulated by the system, and detected a ..
  63. pmc Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm
    Karin Sauer
    Center for Biofilm Engineering, Montana State University Bozeman, Bozeman, Montana, USA
    J Bacteriol 184:1140-54. 2002
    Complementary approaches were employed to characterize transitional episodes in Pseudomonas aeruginosa biofilm development using direct observation and whole-cell protein analysis...
  64. pmc Pseudomonas aeruginosa evasion of phagocytosis is mediated by loss of swimming motility and is independent of flagellum expression
    Eyal Amiel
    Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA
    Infect Immun 78:2937-45. 2010
    b>Pseudomonas aeruginosa is a pathogenic Gram-negative bacterium that causes severe opportunistic infections in immunocompromised individuals; in particular, severity of infection with P...
  65. pmc Direct interaction between sensor kinase proteins mediates acute and chronic disease phenotypes in a bacterial pathogen
    Andrew L Goodman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 23:249-59. 2009
    The genome of the opportunistic pathogen Pseudomonas aeruginosa encodes over 60 two-component sensor kinases and uses several (including RetS and GacS) to reciprocally regulate the production of virulence factors involved in the ..
  66. ncbi Regulation of virulence and antibiotic resistance by two-component regulatory systems in Pseudomonas aeruginosa
    W James Gooderham
    Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
    FEMS Microbiol Rev 33:279-94. 2009
    The Gram-negative opportunistic pathogen Pseudomonas aeruginosa ubiquitously inhabits soil and water habitats and also causes serious, often antibiotic resistant, infections in immunocompromised patients (e.g. cystic fibrosis)...
  67. pmc An essential role for XBP-1 in host protection against immune activation in C. elegans
    Claire E Richardson
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 463:1092-5. 2010
    ..Activation of the PMK-1-mediated response to infection with Pseudomonas aeruginosa induces the XBP-1-dependent UPR...
  68. ncbi The contribution of MvfR to Pseudomonas aeruginosa pathogenesis and quorum sensing circuitry regulation: multiple quorum sensing-regulated genes are modulated without affecting lasRI, rhlRI or the production of N-acyl-L-homoserine lactones
    Eric Déziel
    Department of Surgery, Harvard Medical School, Massachusetts General Hospital, 50 Blossom Street, MA, USA
    Mol Microbiol 55:998-1014. 2005
    The transcriptional regulator MvfR is required for full Pseudomonas aeruginosa virulence, the function of multiple quorum sensing (QS)-regulated virulence factors and the synthesis of 4-hydroxy-2-alkylquinolines (HAQs), including the ..
  69. ncbi Sigma factors in Pseudomonas aeruginosa
    Eric Potvin
    Centre de Recherche sur la Fonction, Structure et Ingénierie des Protéines, Faculte de Medecine, Pavillon Charles Eugène Marchand, Universite Laval, Sainte Foy, Quebec, Canada
    FEMS Microbiol Rev 32:38-55. 2008
    In Pseudomonas aeruginosa, as in most bacterial species, the expression of genes is tightly controlled by a repertoire of transcriptional regulators, particularly the so-called sigma (sigma) factors...
  70. ncbi A four-tiered transcriptional regulatory circuit controls flagellar biogenesis in Pseudomonas aeruginosa
    Nandini Dasgupta
    Department of Medicine Infectious Diseases, University of Florida, Gainesville, FL 32610, USA
    Mol Microbiol 50:809-24. 2003
    The single polar flagellum of Pseudomonas aeruginosa is an important virulence and colonization factor of this opportunistic pathogen...
  71. pmc Functions required for extracellular quinolone signaling by Pseudomonas aeruginosa
    Larry A Gallagher
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    J Bacteriol 184:6472-80. 2002
    ..reduced production of the phenazine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1...
  72. pmc Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors
    Morten Hentzer
    BioCentrum DTU, Technical University of Denmark, DK 2800 Lyngby, Denmark
    EMBO J 22:3803-15. 2003
    ..Compounds that can override communication signals have been found in the marine environment. Using Pseudomonas aeruginosa PAO1 as an example of an opportunistic human pathogen, we show that a synthetic derivate of natural ..
  73. pmc Evolving stealth: genetic adaptation of Pseudomonas aeruginosa during cystic fibrosis infections
    Dao Nguyen
    Department of Medicine, University of Washington School of Medicine, 1959 Northeast Pacific Street, Room G 310, Seattle, WA 98195 7242, USA
    Proc Natl Acad Sci U S A 103:8305-6. 2006
  74. pmc Cooperation and virulence of clinical Pseudomonas aeruginosa populations
    Thilo Köhler
    Department of Microbiology and Molecular Medicine and Service of Infectious Diseases, University Hospital Geneva, 1211 Geneva, Switzerland
    Proc Natl Acad Sci U S A 106:6339-44. 2009
    ..Studies using the opportunistic pathogen Pseudomonas aeruginosa suggest that QS "cheats"-individuals that don't respond to the QS signal, but are still able to use public ..
  75. ncbi Iron uptake regulation in Pseudomonas aeruginosa
    Pierre Cornelis
    Department of Molecular and Cellular Interactions, Laboratory of Microbial Interactions, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium
    Biometals 22:15-22. 2009
    ..aeruginosa will be presented. An interesting feature revealed by this analysis of Fur-regulated genes is the overlap between the iron and the sulfur regulons as well with the quorum sensing system...
  76. pmc Pseudomonas aeruginosa suppresses host immunity by activating the DAF-2 insulin-like signaling pathway in Caenorhabditis elegans
    Eric A Evans
    Department of Genetics, Stanford University School of Medicine, Stanford, California, United States of America
    PLoS Pathog 4:e1000175. 2008
    ..We present evidence that Pseudomonas aeruginosa is able to suppress the expression of a subset of immune defense genes in the animal host Caenorhabditis ..
  77. pmc Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy
    O Lomovskaya
    Microcide Pharmaceuticals, Inc, Mountain View, California 94043, USA
    Antimicrob Agents Chemother 45:105-16. 2001
    ..MexAB-OprM, MexCD-OprJ, MexEF-OprN) that contribute to fluoroquinolone resistance in clinical isolates of Pseudomonas aeruginosa. Secondary assays were developed to identify lead compounds with exquisite activities as inhibitors...
  78. ncbi GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motility
    Roger Simm
    Microbiology and Tumorbiology Center MTC, Karolinska Institutet, Box 280, SE 171 77 Stockholm, Sweden
    Mol Microbiol 53:1123-34. 2004
    ..show that, in the pathogenic organism Salmonella enterica serovar Typhimurium, the nosocomial pathogen Pseudomonas aeruginosa and the commensal species Escherichia coli, GGDEF and EAL domains mediate similar phenotypic changes ..
  79. pmc The pel polysaccharide can serve a structural and protective role in the biofilm matrix of Pseudomonas aeruginosa
    Kelly M Colvin
    Department of Microbiology, University of Washington, Seattle, Washington, United States of America
    PLoS Pathog 7:e1001264. 2011
    ..b>Pseudomonas aeruginosa is a model organism for biofilm studies and produces three extracellular polysaccharides that have been ..
  80. pmc Metabolic network analysis of Pseudomonas aeruginosa during chronic cystic fibrosis lung infection
    Matthew A Oberhardt
    Department of Biomedical Engineering, University of Virginia, Box 800759, Health System, Charlottesville, Virginia 22908, USA
    J Bacteriol 192:5534-48. 2010
    ..we present an integration of expression microarray data with a genome-scale metabolic reconstruction of Pseudomonas aeruginosa in the context of a chronic cystic fibrosis (CF) lung infection. A genome-scale reconstruction of P...
  81. ncbi Dynamics of adaptive microevolution of hypermutable Pseudomonas aeruginosa during chronic pulmonary infection in patients with cystic fibrosis
    Christina Hoboth
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Germany
    J Infect Dis 200:118-30. 2009
    In patients with cystic fibrosis (CF), the emergence of hypermutable Pseudomonas aeruginosa drives the selection of P. aeruginosa variants that are efficiently adapted to the inflamed lungs of these patients.
  82. pmc Biofilms
    Daniel Lopez
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cold Spring Harb Perspect Biol 2:a000398. 2010
    ..well-studied model systems to give an overview of how several organisms form biofilms: Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus...
  83. pmc Quinolones: from antibiotics to autoinducers
    Stephan Heeb
    School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham, UK
    FEMS Microbiol Rev 35:247-74. 2011
    ..b>Pseudomonas aeruginosa and related bacteria produce a number of 2-alkyl-4(1H)-quinolones, some of which exhibit antimicrobial ..
  84. ncbi Pseudomonas aeruginosa biofilms in cystic fibrosis
    Niels Høiby
    Department of Clinical Microbiology 9301, Rigshospitalet, University of Copenhagen, Juliane Maries Vej 22, Copenhagen, Denmark
    Future Microbiol 5:1663-74. 2010
    The persistence of chronic Pseudomonas aeruginosa lung infections in cystic fibrosis (CF) patients is due to biofilm-growing mucoid (alginate-producing) strains...
  85. pmc Prevalence, conservation and functional analysis of Yersinia and Escherichia CRISPR regions in clinical Pseudomonas aeruginosa isolates
    K C Cady
    Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
    Microbiology 157:430-7. 2011
    Here, we report the characterization of 122 Pseudomonas aeruginosa clinical isolates from three distinct geographical locations: Dartmouth Hitchcock Medical Center in New Hampshire, USA, the Charles T...
  86. ncbi Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients
    Thomas Bjarnsholt
    Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Denmark
    Pediatr Pulmonol 44:547-58. 2009
    The present study was undertaken to investigate the appearance and location of Pseudomonas aeruginosa in the cystic fibrosis (CF) lung and in sputum. Samples include preserved tissues of CF patients who died due to chronic P...
  87. ncbi Adaptation of Pseudomonas aeruginosa during persistence in the cystic fibrosis lung
    Michael Hogardt
    Bavarian Health and Food Safety Authority, Oberschleissheim, Germany
    Int J Med Microbiol 300:557-62. 2010
    ..aeruginosa variants, the antipseudomonal treatment, the prediction of the infectious disease state, and the development of efficient vaccines...
  88. ncbi Bacterial hypermutation in cystic fibrosis, not only for antibiotic resistance
    A Oliver
    Servicio de Microbiología and Unidad de Investigación, Hospital Son Dureta, Instituto Universitario de Investigación en Ciencias de la Salud, Palma de Mallorca, Spain
    Clin Microbiol Infect 16:798-808. 2010
    ..Among them, chronic respiratory infection with Pseudomonas aeruginosa in cystic fibrosis (CF) patients was the first natural environment to reveal the high prevalence and ..
  89. pmc Two genetic loci produce distinct carbohydrate-rich structural components of the Pseudomonas aeruginosa biofilm matrix
    Lisa Friedman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    J Bacteriol 186:4457-65. 2004
    b>Pseudomonas aeruginosa forms biofilms, which are cellular aggregates encased in an extracellular matrix...
  90. pmc Emergence of Pseudomonas aeruginosa strains producing high levels of persister cells in patients with cystic fibrosis
    Lawrence R Mulcahy
    Department of Biology and Antimicrobial Discovery Center, Northeastern University, Boston, Massachusetts 02115, USA
    J Bacteriol 192:6191-9. 2010
    The majority of cystic fibrosis (CF) patients succumb to a chronic infection of the airway with Pseudomonas aeruginosa. Paradoxically, pathogenic strains are often susceptible to antibiotics, but the infection cannot be eradicated with ..
  91. ncbi The pyocins of Pseudomonas aeruginosa
    Yvon Michel-Briand
    Department of Bacteriology, Faculty of Medicine, Jean Minjoz Hospital, Boulevard Fleming, 25030, Besancon, France
    Biochimie 84:499-510. 2002
    Pyocins are produced by more than 90% of Pseudomonas aeruginosa strains and each strain may synthesise several pyocins. The pyocin genes are located on the P...
  92. pmc Interaction between bacteriophage DMS3 and host CRISPR region inhibits group behaviors of Pseudomonas aeruginosa
    Michael E Zegans
    Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
    J Bacteriol 191:210-9. 2009
    ..We observed that lysogenic infection of Pseudomonas aeruginosa PA14 with bacteriophage DMS3 inhibits biofilm formation and swarming motility, both important bacterial ..
  93. ncbi Type III protein secretion is associated with death in lower respiratory and systemic Pseudomonas aeruginosa infections
    A Roy-Burman
    Department of Anesthesia and Perioperative Care, Cardiovascular Research Institute, University of California, San Francisco, CA 94143-0542, USA
    J Infect Dis 183:1767-74. 2001
    The ability of Pseudomonas aeruginosa to secrete specific toxins using the type III-mediated pathway has been reported...
  94. ncbi Paraoxonase 2 is down-regulated by the Pseudomonas aeruginosa quorumsensing signal N-(3-oxododecanoyl)-L-homoserine lactone and attenuates oxidative stress induced by pyocyanin
    Sven Horke
    Department of Pharmacology, University Medicine Mainz, Obere Zahlbacher Strasse 67, 55131 Mainz, Germany
    Biochem J 426:73-83. 2010
    Two virulence factors produced by Pseudomonas aeruginosa are pyocyanin and N-(3-oxododecanoyl)-L-homoserine lactone (3OC12). Pyocyanin damages host cells by generating ROS (reactive oxygen species)...
  95. pmc Pseudomonas aeruginosa activates caspase 1 through Ipaf
    Edward A Miao
    Institute for Systems Biology, Seattle, WA 98103, USA
    Proc Natl Acad Sci U S A 105:2562-7. 2008
    ..We demonstrate here that Pseudomonas aeruginosa activates caspase 1 and induces IL-1beta secretion in infected macrophages. While live, virulent P...
  96. pmc mexEF-oprN multidrug efflux operon of Pseudomonas aeruginosa: regulation by the MexT activator in response to nitrosative stress and chloramphenicol
    Hossam Fetar
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Antimicrob Agents Chemother 55:508-14. 2011
    A null mutation in the mexS gene of Pseudomonas aeruginosa yielded an increased level of expression of a 3-gene operon containing a gene, xenB, whose product is highly homologous to a xenobiotic reductase in Pseudomonas fluorescens shown ..
  97. pmc The Pseudomonas aeruginosa pathogenicity island PAPI-1 is transferred via a novel type IV pilus
    Michelle Qiu Carter
    Harvard Medical School, Department of Microbiology and Molecular Genetics, 200 Longwood Ave, Boston, MA 02115, USA
    J Bacteriol 192:3249-58. 2010
    b>Pseudomonas aeruginosa is a major cause of nosocomial infections, particularly in immunocompromised patients or in individuals with cystic fibrosis. The notable ability of P...
  98. pmc Transcriptomic analysis reveals a global alkyl-quinolone-independent regulatory role for PqsE in facilitating the environmental adaptation of Pseudomonas aeruginosa to plant and animal hosts
    Giordano Rampioni
    School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, UK
    Environ Microbiol 12:1659-73. 2010
    The quorum sensing (QS) system of Pseudomonas aeruginosa constitutes a sophisticated genome-wide gene regulatory network employing both N-acylhomoserine lactone and 2-alkyl-4-quinolone (AQ) signal molecules...
  99. ncbi Early adaptive developments of Pseudomonas aeruginosa after the transition from life in the environment to persistent colonization in the airways of human cystic fibrosis hosts
    Martin Holm Rau
    Department of Systems Biology, Technical University of Denmark, Building 301, 2800 Lyngby, Denmark
    Environ Microbiol 12:1643-58. 2010
    b>Pseudomonas aeruginosa is an opportunistic pathogen ubiquitous to the natural environment but with the capability of moving to the host environment...
  100. pmc Quorum sensing inhibition selects for virulence and cooperation in Pseudomonas aeruginosa
    Thilo Köhler
    Service of Infectious Diseases, University Hospital Geneva and Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland
    PLoS Pathog 6:e1000883. 2010
    ..Virulence and cooperative social behaviour of the bacterium Pseudomonas aeruginosa rely on the quorum-sensing (QS) controlled production of extracellular products (public goods)...
  101. pmc Pseudomonas aeruginosa virulence and therapy: evolving translational strategies
    Jeffrey L Veesenmeyer
    Department of Microbiology Immunology, Northwestern University, Chicago, IL, USA
    Crit Care Med 37:1777-86. 2009
    Although most reviews of Pseudomonas aeruginosa therapeutics focus on antibiotics currently in use or in the pipeline, we review evolving translational strategies aimed at using virulence factor antagonists as adjunctive therapies.

Research Grants75

  1. Sutureless Laparoscopic Partial Nephrectomy Using Laser Tissue Welding: Preclinic
    Yasmin Wadia; Fiscal Year: 2013
    ..contaminated wounds of drug- resistant super bugs like Methicillin-Resistant Staphylococcus aureus (MRSA), Streptococcus pyogenes and Pseudomonas aeruginosa. Fibro-optic capable: Minimally invasive laparoscopic &robotic applications.
  2. Targeting superbugs: discovery and development of new broad-spectrum lipopeptides
    Jian Li; Fiscal Year: 2013
    ..Numerous hospitals worldwide have experienced outbreaks of infections caused by multidrug-resistant (MDR) Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, Enterobacter species, Enterococcus faecium and ..
  3. Novel Bioconjugate Vaccines to Prevent Staphylococcus aureus Infection
    JEAN CLAIRE LEE; Fiscal Year: 2013
    ..aureus capsule type 5 polysaccharide (CP5) vaccine covalently linked to the carrier protein EPA (nontoxic Pseudomonas aeruginosa exoprotein A)...
  4. Mechanism Underlying Nitrite Sensitivity of Mucoid Pseudomonas in COPD
    Daniel J Hassett; Fiscal Year: 2013
    ..COPD) that often suffer from airway infection by opportunistic bacteria, the most prevalent of which is Pseudomonas aeruginosa (PA)...
  5. The Electricidal Effect, a Novel Anti-Biofilm Strategy
    Robin Patel; Fiscal Year: 2013
    ..submission demonstrated that electrical currents of 20 to 2000 <A substantially reduced established Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis biofilms, a phenomenon we termed the "electricidal ..
  6. Optimization of Neoglycoside Antibiotics for Nosocomial Pathogens and Select Agen
    Arnold Louie; Fiscal Year: 2013
    ..With Pseudomonas aeruginosa and Acinetobacter spp...
  7. Inhibitors of isoprenoid synthesis for antibacterial therapy
    Donald T Moir; Fiscal Year: 2013
    DESCRIPTION: Pseudomonas aeruginosa is a common and extremely virulent cause of serious infections in immune- compromised/suppressed patients (e.g...
  8. Use of Phosphodiesterase Inhibitors to Evaluate the Pathobiology of CF
    JENNIFER L TAYLOR-COUSAR; Fiscal Year: 2013
    ..and its downstream effects, including disordered glycosylation, predisposition to adherence by Pseudomonas aeruginosa and consequent inflammation...
  9. Analogs of GTP as novel inhibitors of bacterial c-di-GMP-synthesizing enzymes
    George E Wright; Fiscal Year: 2010
    ..and LCMS methods;2, test GTP analogs for their ability to inhibit cyclization of GTP to c-di- GMP by the Pseudomonas aeruginosa DGC "PA3702";a coupled assay measuring pyrophosphate release and a HPLC assay measuring product formation ..
  10. Mechanism of Translation Elongation Factor 2 Inhibition by Bacterial Toxins
    Terri Goss Kinzy; Fiscal Year: 2010
    Toxins produced by Corynebacterium diphtheriae (diphtheria toxin) and Pseudomonas aeruginosa (exotoxin A) both result in host cell death by the inhibition of protein synthesis...
  11. ENZYMOLOGY OF ANTIBIOTIC RESISTANCE
    Richard Armstrong; Fiscal Year: 2009
    ..encoded resistance proteins are extant in several select human pathogens, for example, (FosA) in Pseudomonas aeruginosa, (FosB) in Staphylococcus aureus and Bacillus anthracis, and (FosX) in Brucella melitensis, Listeria ..
  12. Mechanisms of adaptive immunity to P. aeruginosa in the lung
    Gregory P Priebe; Fiscal Year: 2013
    b>Pseudomonas aeruginosa is an important cause of serious infections in humans with a predilection for lung infections in the form of either acute pneumonia in hospitalized patients (usually associated with mechanical ventilation) or ..
  13. ROLE OF MUC1 IN THE GENESIS OF ALLERGIC ASTHMA
    Kwang Chul Kim; Fiscal Year: 2010
    ..Recently we found that it is a receptor for Pseudomonas aeruginosa and binding of bacteria was mediated through flagellin resulting in tyrosine phosphorylation of its ..
  14. MNEI/SerpinB1: A modulator of innate pulmonary defense
    EILEEN REMOLD-O'DONNELL; Fiscal Year: 2010
    ..The mnei deficient mice fail to clear Pseudomonas aeruginosa and have increased proinflammatory cytokines and depletion of surfactant protein-D (SP-D), a known target ..
  15. Cyclic AMP Signaling in Pseudomonas aeruginosa Virulence
    Matthew C Wolfgang; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Pseudomonas aeruginosa is an opportunistic human pathogen that is responsible for nearly 10% of hospital-acquired infections and is the primary cause of morbidity and mortality in cystic fibrosis ..
  16. X-ray Studies of Sugar-Modifying Enzymes
    Hazel Holden; Fiscal Year: 2013
    ..rare sugar, D-ManNAc3NAcA, has been isolated from the B-band O-antigen of the opportunistic bacterium Pseudomonas aeruginosa, a major source of nosocomial infections and in the A-band trisaccharide of the bacterium Bordetella ..
  17. Mechanisms to restrain social cheating from quorum sensing in Pseudomonas aerugin
    AJAI DANDEKAR; Fiscal Year: 2013
    ..career award is for support to transition to a career of independent investigation in the field of Pseudomonas aeruginosa quorum sensing...
  18. Counter-Regulation of TLR-Driven Mucosal Inflammation
    Erik P Lillehoj; Fiscal Year: 2010
    ..characterized by mucus hypersecretion, reduced mucociliary clearance of inhaled bacteria, particularly Pseudomonas aeruginosa (PA), and chronic airway inflammation...
  19. Bacterial Immunosuppression: Host Target Identification Through Chemical Probes
    Kim D Janda; Fiscal Year: 2013
    ..With respect to Pseudomonas aeruginosa, N-3- oxo-dodecanoyl-L-homoserine lactone (C12) and N-butanoyl-L-homoserine lactone are utilized by the ..
  20. Novel biofilm polymicrobial infection-resistant implants for otologic application
    Paul Stoodley; Fiscal Year: 2010
    ..We have demonstrated in Phase I that we can suppress biofilm formation and planktonic growth of Pseudomonas aeruginosa and Staphylococcus aureus for an extended period of time...
  21. Regulation of NETosis in antibacterial lung defense
    EILEEN REMOLD-O'DONNELL; Fiscal Year: 2013
    ..failure to eradicate bacterial (Streptococcus pneumonia, Haemophilus influenza, Staphylococcus aureus, Pseudomonas aeruginosa) and viral (rhinovirus, influenza virus) infections are responsible for a tremendous burden of ..
  22. Exploiting deep ocean biology for biomedical discovery
    David C Rowley; Fiscal Year: 2011
    ..strains of multiple drug resistant Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae...
  23. Structure and Mechanism of SAM-responsive Riboswitches
    Robert T Batey; Fiscal Year: 2013
    ..in a number of medically important pathogens including Listeria monocytogenes, Staphylococcus aureus, Pseudomonas aeruginosa, and Mycobacterium tuberculosis making them of great interest as novel targets for designing antimicrobial ..
  24. Polymicrobial interactions in the lung.
    JENNIFER MELINDA BOMBERGER; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Pseudomonas aeruginosa is an opportunistic pathogen and an important pathogen in patients with chronic obstructive pulmonary disorder (COPD), community acquired pneumonia (CAP), ventilator-associated ..
  25. Mechanisms of Pseudomonas Resistance to Membrane Permeabilization by SP-A
    Gee W Lau; Fiscal Year: 2012
    ..Recently, we have reported that SP-A also directly kills Pseudomonas aeruginosa (PA) in a macrophage-independent manner, by increasing the permeability of bacterial membranes...
  26. Cytoskeletal Keratins in Epithelial Immunity to Bacterial Keratitis
    K P CONNIE TAM; Fiscal Year: 2013
    ..their structural interactions with bacterial membranes, and by identifying their bacterial receptors on Pseudomonas aeruginosa, a major corneal pathogen. Aim 3 will investigate the role of KDAMPs in protecting the cornea against P...
  27. Challenges in beta-Lactamase Mediated Resistance
    Robert A Bonomo; Fiscal Year: 2013
    ..Acinetobacter baumannii, and Pseudomonas aeruginosa. In this proposal we will continue our work with boronic acid transition state inhibitors (BATSIs) as ..
  28. Regulation of Streptomyces differentiation and antibiotic production by SoxR
    Monica Chander; Fiscal Year: 2010
    ..This has been confirmed in Pseudomonas aeruginosa where SoxR senses endogenous antibiotics and controls the expression of genes involved in their transport ..
  29. Molecular mechanisms of pathogen recognition by the Nod-like receptor NLRC4
    Fayyaz S Sutterwala; Fiscal Year: 2012
    Project Summary Pseudomonas aeruginosa causes a variety of acute infections, such as ventilator-associated pneumonias, burn superinfections, neutropenic bacteremia, and medical device-related infections. In addition, P...
  30. Mechanisms of trauma and hemorrhage-induced impairment of innate immunity
    Xiao Di Tan; Fiscal Year: 2013
    ..will study whether hemorrhage-induced alteration of Atg12 is associated with increase in susceptibility to Pseudomonas aeruginosa infection in lungs...
  31. Interactions between P. aeruginosa and autophagy in Airway epithelial cells
    INDHIRA H DE LA ROSA; Fiscal Year: 2013
    ..DESCRIPTION (provided by applicant): The goal of our research proposal is to understand the interactions of Pseudomonas aeruginosa (P...
  32. Pathogensis of Bacterial corneal infection
    Gerald B Pier; Fiscal Year: 2013
    ..Specifically, this application will focus on infections caused by Pseudomonas aeruginosa and Staphylococcus aureus...
  33. Cell Based Therapies for Cystic Fibrosis
    Tracey L Bonfield; Fiscal Year: 2012
    ..The morbidities are associated with inefficient resolution of pulmonary infection with Pseudomonas aeruginosa and the ensuing inflammatory response...
  34. Polymicrobial Interactions in the Lung
    JENNIFER MELINDA BOMBERGER; Fiscal Year: 2013
    b>Pseudomonas aeruginosa is an opportunistic pathogen and an important pathogen in patients with chronic obstructive pulmonary disorder (COPD), community acquired pneumonia (CAP), ventilator-associated pneumonia (VAP), non-CF bronchiectasis ..
  35. Innovative technologies to effectively treat Multi-drug resistant and/or biofilm-
    Jie Wen; Fiscal Year: 2010
    ..baumannii, Klebsiella pneumoniae, methicillin-resistant Staphylococcus aureus, and multi-drug resistant Pseudomonas aeruginosa. Infections caused by these multi-drug resistant organisms appear to result in significant morbidity and ..
  36. Mechanisms of flagellin-induced protection against microbial keratitis
    Fu Shin X Yu; Fiscal Year: 2013
    ..innate immunity and render resistance to a broad spectrum of keratitis causing pathogens, including Pseudomonas aeruginosa, Candida albicans and Aspergillus fumigates...
  37. Host-associated regulation of P. aeruginosa colonization and virulence
    Deborah A Hogan; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Pseudomonas aeruginosa is among the most dangerous Gram-negative opportunistic pathogens. In the compromised lung, P...
  38. Molecular Basis of Bacterial Quorum Sensing Gene Regulation
    MAIR E CHURCHILL; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Opportunistic infections by Pseudomonas aeruginosa are a major cause of death in cystic fibrosis patients and immune-compromised individuals...
  39. Pathogen-Specific Regulation of Protein Assembly
    ALLEN BERNARD REITZ; Fiscal Year: 2010
    ..enterocolitica to Plasmodium falciparum, Pseudomonas aeruginosa, Vibrio cholerae, and Toxoplasma gondii...
  40. Genes Mediating Innate Immune Suppression by Hypercapnia in Mammals and Flies
    PETER H SPORN; Fiscal Year: 2013
    ..human macrophages, and in vivo using adult Drosophila and lung inflammatory cells obtained from mice with Pseudomonas aeruginosa pneumonia...
  41. CELLULAR AND MOLECULAR BIOLOGY OF CHOLESTEATOMA
    RICHARD ARTHUR CHOLE; Fiscal Year: 2013
    ..of bacterial-host interactions in the pathogenesis and virulence of cholesteatomas infected with Pseudomonas aeruginosa (PA)...
  42. CAP37 and ocular inflammation
    HELOISE ANNE PEREIRA; Fiscal Year: 2011
    ..for host defense in ocular infection we will use CAP37 and its bioactive peptides in a murine model of Pseudomonas aeruginosa keratitis to determine in vivo efficacy...
  43. Identifying novel anti-infectives by high through-put screening in whole animals
    Frederick M Ausubel; Fiscal Year: 2013
    ..To establish proof-of-principle, we seek funding for discovering new anti-infectives against Pseudomonas aeruginosa, one of several gram-negative bacteria that have recently emerged in a multi-drug resistant form for which ..
  44. Aminoglycoside potentiators for P. aeruginosa therapy
    Donald T Moir; Fiscal Year: 2013
    DESCRIPTION: Pseudomonas aeruginosa is a common cause of serious infections in patients with immune deficiency (e.g., HIV and cancer), cystic fibrosis (CF), on mechanical ventilation or with burn wounds. While P...
  45. Augmentation of Innate Anti-Microbial Immunity by TLR4 Agonists
    Edward R Sherwood; Fiscal Year: 2013
    ..4 (TLR4) agonist monophosphoryl lipid A (MPLA) is effective in improving innate host resistance to Pseudomonas aeruginosa burn wound sepsis in mice...
  46. Understanding of a Family of Bacterial Transcription Factors
    Hwan Youn; Fiscal Year: 2012
    ..PUBLIC HEALTH RELEVANCE: The CRP homologs found in bacterial pathogens such as Pseudomonas aeruginosa and Listeria monocytogenes act as virulence factors...
  47. Mechanism of heme regulation of a P. aeruginosa non-coding RNA
    AMANDA GAIL OGLESBY-SHERROUSE; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infection in compromised individuals, including neutropenic cancer patients and individuals with cystic fibrosis (CF)...
  48. Regulation of Pore Formation by Type III Secretion System
    Gloria I Viboud; Fiscal Year: 2010
    ..enteropathogenic Escherichia coli as well agents causing severe pneumonia, like the opportunistic bacteria Pseudomonas aeruginosa, or the deadly Yersinia pestis. Interestingly, Y...
  49. Probing localiztion, interactions, and effector properties of Shigella lpaD
    NICHOLAS EUGENE DICKENSON; Fiscal Year: 2010
    ..numerous other important pathogens such as Yersinia pestis (plague), Salmonella (gastroenteritis), and Pseudomonas aeruginosa (lung infection) utilize the type III secretion system (TTSS) as a means of subverting the normal ..
  50. Hypercapnia and neutrophil function in pulmonary host defense
    Khalilah L Gates; Fiscal Year: 2013
    ..Furthermore, prolonged hypercapnia in the murine Pseudomonas aeruginosa pneumonia model suggests that hypercapnia leads to a decrease in bacterial clearance and increased ..
  51. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2012
    ..of Escherichia coli LpxC are relatively inactive against divergent LpxC enzymes, especially that from Pseudomonas aeruginosa, the leading cause of death in cystic fibrosis patients...
  52. Preventing Burn Wound Infection Using a Novel Free Radical Sterilization System
    BENJAMIN DAVID SADOWITZ; Fiscal Year: 2011
    ..thickness burn injury to create a polymicrobial burn wound infection with Staphylococcus aureus and Pseudomonas aeruginosa, two of the common organisms causing burn wound infection in humans...
  53. Iron and O2 in the Regulation of P. aeruginosa Virulence
    Michael L Vasil; Fiscal Year: 2012
    b>Pseudomonas aeruginosa (PA) is a malicious opportunistic pathogen both in terms of the severity and the outcome of infections it causes. A significant proportion of patients with cystic fibrosis (CF) are colonized at an early age (<1yr...
  54. Genetic control of denitrification in Pseudomonas aeruginosa
    Valley J Stewart; Fiscal Year: 2010
    b>Pseudomonas aeruginosa infection is the major cause of morbidity and mortality in cystic fibrosis...
  55. Development of an antimicrobial peptide therapeutic for Pseudomonas infections
    HELOISE ANNE PEREIRA; Fiscal Year: 2011
    ..and CAP372o-44 Ser42, have potent antimicrobial activity for Gram-negative bacteria, including Pseudomonas aeruginosa and Acinetobacter baumannii...
  56. Reducing wound bioburden and biofilm formation using a nanoscale wound surface en
    Jonathan F McAnulty; Fiscal Year: 2013
    ..PLGA beads to prevent a biofilm from forming and to stimulate dissolution of existing biofilms of Pseudomonas aeruginosa and Staphylococcus aureus in vitro ...
  57. Geobiological approaches to understanding pulmonary infections in situ
    Dianne K Newman; Fiscal Year: 2013
    ..These methods will be refined and validated using planktonic and biofilm cultures of Pseudomonas aeruginosa and subsequently applied to CF patient samples in collaboration with clinicians from Children's ..
  58. GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLI
    Valley J Stewart; Fiscal Year: 2010
    ..g., Vibrio cholerae) or the NarX-NarL system (e.g., Pseudomonas aeruginosa)...
  59. Design of New Antimicrobials
    Robert S Hodges; Fiscal Year: 2012
    ..classes of antimicrobial peptides in the membrane;5) demonstrate in vivo efficacy of our peptides against Pseudomonas aeruginosa challenge in two animal models;6) extend our studies to animal models of other serious bacterial ..
  60. Mechanism of cellulose synthesis and transport across biological membranes
    Jochen Zimmer; Fiscal Year: 2013
    ..For example, chronic biofilm infections of Pseudomonas aeruginosa are commonly found in cystic fibrosis patients...
  61. Xenon MRI assessment of response to cystic fibrosis therapies
    IULIAN CONSTANTIN RUSET; Fiscal Year: 2012
    ..care;simultaneous use of intravenous and inhaled antibiotics;number of antibiotics used to treat pseudomonas aeruginosa;continuous infusion of betalactam antibiotics;and duration of antibiotic treatment...
  62. ARRA - NHLBI Lung Cohorts Sequencing Project
    Michael Bamshad; Fiscal Year: 2009
    ..Our phenotypes include: severity of lung disease in cystic fibrosis, time to acquisition of Pseudomonas aeruginosa (Pa) in cystic fibrosis, severity of lung disease in asthma, rate of decline of lung function in chronic ..
  63. WEILL CORNELL PROGRAM OF EXCELLENCE IN GENE THERAPY
    Ronald Crystal; Fiscal Year: 2005
    ..Rafii); Project 3*- Immunization against Pseudomonas aeruginosa by capsid modified Ad vectors expressing Pseudomonas OprF (S...
  64. Susceptibility Testing, Treatment Choices, and Outcomes of MDR-GNB Infections
    Lisa Maragakis; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Multidrug resistant (MDR) Gram-negative bacteria including Pseudomonas aeruginosa, Acinetobacter species, and Klebsiella pneumoniae are emerging pathogens that are increasingly the cause of a variety ..
  65. Selenium Coated Dialysis Catheters for Reduced Biofilm Formation
    Eric Tobin; Fiscal Year: 2009
    ..gt;90%) reduction in biofilm formation for both gram positive (Staphylococcus aureus) and gram negative (Pseudomonas aeruginosa) bacteria on selenium coated polyurethane catheter material...
  66. Pseudomonas aeruginosa genomic islands and virulence factors
    VANDERLENE LIU KUNG; Fiscal Year: 2011
    ..b>Pseudomonas aeruginosa is an environmentally ubiquitous bacterium that causes disease in both plants and animals, including ..
  67. Etiology, Epidemiology, and Clinical Outcomes of Health Care Associated Pneumonia
    Karl Madaras Kelly; Fiscal Year: 2009
    ..identify risk factors for MDR bacteria including Methicillin-Resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, compared to non-MDR bacteria and develop models that accurately identify patients infected with MDR...
  68. The Pseudomonas Aeruginosa Biofilm Dispersion Response
    David Davies; Fiscal Year: 2009
    ....
  69. Impact of Fluoroquinolone resistance on Pseudomonas virulence and patient outcome
    Annie Wong Beringer; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Pseudomonas aeruginosa (PA) is a bacterium that poses an immediate threat to public health because of its leading role in causing nosocomial infections, emergence of multidrug-resistance, virulence ..
  70. Role of Burkholderia Cenocepacia Adhesin, AdhA, in Cystic Fibrosis Infections
    Joanna B Goldberg; Fiscal Year: 2010
    ..b>Pseudomonas aeruginosa is responsible for most lung infections, affecting >80% of CF patients;however, bacteria belonging to ..
  71. Stringent control of virulence gene expression in Pseudomonas aeruginosa
    Martin Schuster; Fiscal Year: 2009
    Chronic infections by the opportunistic pathogen Pseudomonas aeruginosa are the major cause of morbidity in patients suffering from cystic fibrosis (CF). In the CF lung, P...
  72. Biofilm Formation And P. aeruginosa infection of the eye
    Michael Zegans; Fiscal Year: 2006
    ..b>Pseudomonas aeruginosa (PA) is one of the most common causes of bacterial keratitis...
  73. Analysis of P. aeruginosa genome diversity and evolution
    Stephen Lory; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): Pseudomonas aeruginosa, a common inhabitant of water and soil, is also responsible for a range of serious human infections including the chronic respiratory disease of patients with ..
  74. ENZYMOLOGY OF ANTIBIOTIC RESISTANCE
    Richard Armstrong; Fiscal Year: 2007
    ..elements have now been shown to exist in the genomes of several pathogenic microorganisms including, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus anthrasis, Brucella melitensis, Listeria monocytogenes and Clostridium ..
  75. Non-clinical Development of Bactericidal Proteins Targeting Plague
    DAVID WILLIAM MARTIN; Fiscal Year: 2010
    ..have efficacy when administered intraperitoneally or intravenously to mice infected systemically with Pseudomonas aeruginosa. This Phase 2 application proposes to characterize the immunogenicity of R-type pyocins in mice and then ..