burkholderia mallei

Summary

Summary: A species of gram-negative bacteria parasitic on HORSES and DONKEYS causing GLANDERS, which can be transmitted to humans.

Top Publications

  1. ncbi mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    Nat Protoc 1:162-9. 2006
  2. pmc Burkholderia mallei cellular interactions in a respiratory cell model
    Gregory C Whitlock
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555 1070, USA
    J Med Microbiol 58:554-62. 2009
  3. ncbi Molecular insights into Burkholderia pseudomallei and Burkholderia mallei pathogenesis
    Edouard E Galyov
    Department of Infection, Immunity and Inflammation, MSB, University of Leicester, Leicester LE1 9HN, United Kingdom
    Annu Rev Microbiol 64:495-517. 2010
  4. pmc Present and future therapeutic strategies for melioidosis and glanders
    D Mark Estes
    Department of Microbiology and Immunology, Department of Pathology and The Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555 1070, USA
    Expert Rev Anti Infect Ther 8:325-38. 2010
  5. pmc In Vitro and In Vivo studies of monoclonal antibodies with prominent bactericidal activity against Burkholderia pseudomallei and Burkholderia mallei
    Shimin Zhang
    Department of Environmental and Infectious Disease Sciences, American Registry of Pathology, Armed Forces Institute of Pathology, Washington, DC 20306, USA
    Clin Vaccine Immunol 18:825-34. 2011
  6. ncbi Protective efficacy of heat-inactivated B. thailandensis, B. mallei or B. pseudomallei against experimental melioidosis and glanders
    Mitali Sarkar-Tyson
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, SP4 OJQ, UK
    Vaccine 27:4447-51. 2009
  7. pmc Protection from pneumonic infection with burkholderia species by inhalational immunotherapy
    Andrew Goodyear
    Department of Microbiology, Immunology and Pathology, Colorado State University, Ft Collins, CO 80523, USA
    Infect Immun 77:1579-88. 2009
  8. ncbi Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents
    Chris Druar
    Cangene Corporation, Mississauga, ON, Canada
    FEMS Immunol Med Microbiol 52:78-87. 2008
  9. pmc Critical protective role for MCP-1 in pneumonic Burkholderia mallei infection
    Andrew Goodyear
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Ft Collins, CO 80523, USA
    J Immunol 184:1445-54. 2010
  10. ncbi A disruption of ctpA encoding carboxy-terminal protease attenuates Burkholderia mallei and induces partial protection in CD1 mice
    Aloka B Bandara
    Center for Molecular Medicine and Infectious Diseases, Virginia Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 0342, USA
    Microb Pathog 45:207-16. 2008

Detail Information

Publications144 found, 100 shown here

  1. ncbi mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    Nat Protoc 1:162-9. 2006
    ..This first chromosome integration system in B. mallei provides an important contribution to the genetic tools emerging for Burkholderia spp. Vectors are available for gene complementation and expression, and gene fusion analyses...
  2. pmc Burkholderia mallei cellular interactions in a respiratory cell model
    Gregory C Whitlock
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555 1070, USA
    J Med Microbiol 58:554-62. 2009
    b>Burkholderia mallei is a facultative intracellular pathogen that survives and replicates in phagocytic cell lines. The bacterial burden recovered from naïve BALB/c mice infected by intranasal delivery indicated that B...
  3. ncbi Molecular insights into Burkholderia pseudomallei and Burkholderia mallei pathogenesis
    Edouard E Galyov
    Department of Infection, Immunity and Inflammation, MSB, University of Leicester, Leicester LE1 9HN, United Kingdom
    Annu Rev Microbiol 64:495-517. 2010
    Burkholderia pseudomallei and Burkholderia mallei are closely related gram-negative bacteria that can cause serious diseases in humans and animals...
  4. pmc Present and future therapeutic strategies for melioidosis and glanders
    D Mark Estes
    Department of Microbiology and Immunology, Department of Pathology and The Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555 1070, USA
    Expert Rev Anti Infect Ther 8:325-38. 2010
    Burkholderia pseudomallei and Burkholderia mallei are the causative agents of melioidosis and glanders, respectively. Both Gram-negative pathogens are endemic in many parts of the world...
  5. pmc In Vitro and In Vivo studies of monoclonal antibodies with prominent bactericidal activity against Burkholderia pseudomallei and Burkholderia mallei
    Shimin Zhang
    Department of Environmental and Infectious Disease Sciences, American Registry of Pathology, Armed Forces Institute of Pathology, Washington, DC 20306, USA
    Clin Vaccine Immunol 18:825-34. 2011
    ..has developed more than a hundred mouse monoclonal antibodies (MAbs) against Burkholderia pseudomallei and Burkholderia mallei. These antibodies have been categorized into different groups based on their specificities and the ..
  6. ncbi Protective efficacy of heat-inactivated B. thailandensis, B. mallei or B. pseudomallei against experimental melioidosis and glanders
    Mitali Sarkar-Tyson
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, SP4 OJQ, UK
    Vaccine 27:4447-51. 2009
    Burkholderia pseudomallei and Burkholderia mallei are gram-negative bacilli that are the causative agents of melioidosis and glanders, respectively. Both humans and animals are susceptible to both diseases...
  7. pmc Protection from pneumonic infection with burkholderia species by inhalational immunotherapy
    Andrew Goodyear
    Department of Microbiology, Immunology and Pathology, Colorado State University, Ft Collins, CO 80523, USA
    Infect Immun 77:1579-88. 2009
    b>Burkholderia mallei and B. pseudomallei are important human pathogens and cause the diseases glanders and melioidosis, respectively...
  8. ncbi Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents
    Chris Druar
    Cangene Corporation, Mississauga, ON, Canada
    FEMS Immunol Med Microbiol 52:78-87. 2008
    ..However, these ELISAs had low diagnostic accuracy in endemic regions, possibly due to previous patient exposure to B. pseudomallei...
  9. pmc Critical protective role for MCP-1 in pneumonic Burkholderia mallei infection
    Andrew Goodyear
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Ft Collins, CO 80523, USA
    J Immunol 184:1445-54. 2010
    b>Burkholderia mallei is a gram-negative bacterial pathogen of domestic equidae and humans that can cause severe, rapidly life-threatening pneumonic infections...
  10. ncbi A disruption of ctpA encoding carboxy-terminal protease attenuates Burkholderia mallei and induces partial protection in CD1 mice
    Aloka B Bandara
    Center for Molecular Medicine and Infectious Diseases, Virginia Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 0342, USA
    Microb Pathog 45:207-16. 2008
    b>Burkholderia mallei is the etiologic agent of glanders in solipeds (horses, mules and donkeys), and incidentally in carnivores and humans. Little is known about the molecular mechanisms of B. mallei pathogenesis...
  11. ncbi Comparison of four selective media for the isolation of Burkholderia mallei and Burkholderia pseudomallei
    Mindy B Glass
    Bacterial Zoonoses Branch, Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vector Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Am J Trop Med Hyg 80:1023-8. 2009
    Currently there are no commercially available selective media indicated for the isolation of Burkholderia mallei and Burkholderia pseudomallei. Ashdown's agar, a custom selective medium for isolation of B...
  12. pmc Host immunity in the protective response to vaccination with heat-killed Burkholderia mallei
    Gregory C Whitlock
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA
    BMC Immunol 9:55. 2008
    We performed initial cell, cytokine and complement depletion studies to investigate the possible role of these effectors in response to vaccination with heat-killed Burkholderia mallei in a susceptible BALB/c mouse model of infection.
  13. pmc An allelic exchange system for compliant genetic manipulation of the select agents Burkholderia pseudomallei and Burkholderia mallei
    Mohamad A Hamad
    Department of Microbiology, University of Colorado School of Medicine, Mail Stop 8333, PO Box 6511, Aurora, CO 80045, USA
    Gene 430:123-31. 2009
    ..pseudomallei, and one mutant in B. mallei. This vector significantly expands the number of available tools that are select-agent compliant for the genetic manipulation of B. pseudomallei and B. mallei...
  14. ncbi Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice
    Ricky L Ulrich
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702, USA
    Vaccine 23:1986-92. 2005
    b>Burkholderia mallei is an obligate mammalian pathogen that causes the zoonotic disease glanders. Two live attenuated B...
  15. ncbi Type VI secretion is a major virulence determinant in Burkholderia mallei
    Mark A Schell
    Department of Microbiology, University of Georgia, Athens, GA 30602, USA
    Mol Microbiol 64:1466-85. 2007
    b>Burkholderia mallei is a host-adapted pathogen and a category B biothreat agent. Although the B. mallei VirAG two-component regulatory system is required for virulence in hamsters, the virulence genes it regulates are unknown...
  16. pmc Genomic islands from five strains of Burkholderia pseudomallei
    Apichai Tuanyok
    Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011 5640, USA
    BMC Genomics 9:566. 2008
    ..The purpose of this study was to examine the role of genomic islands (GIs) as sources of genomic diversity in this species...
  17. ncbi Outer membrane proteome of Burkholderia pseudomallei and Burkholderia mallei from diverse growth conditions
    Mark A Schell
    Department of Microbiology, University of Georgia, Athens, Georgia 30602, United States
    J Proteome Res 10:2417-24. 2011
    b>Burkholderia mallei and Burkholderia pseudomallei are closely related, aerosol-infective human pathogens that cause life-threatening diseases...
  18. ncbi DNA microarray-based detection and identification of Burkholderia mallei, Burkholderia pseudomallei and Burkholderia spp
    Gernot Schmoock
    Institute of Bacterial Infections and Zoonoses at the Federal Research Institute for Animal Health Friedrich Loeffler Institut, Naumburger Strasse 96a, 07743 Jena, Germany
    Mol Cell Probes 23:178-87. 2009
    ..A representative strain panel comprising 44 B. mallei, 32 B. pseudomallei isolates, and various Burkholderia type strains were examined to validate the test. Assay specificity was determined by examination of 40 non-Burkholderia strains...
  19. ncbi Pathogenesis of Burkholderia pseudomallei and Burkholderia mallei
    Joseph C Larsen
    Defense Threat Reduction Agency Joint Science and Technology Office for Chemical and Biological Defense, 8725 John Kingman Road, Fort Belvoir, VA 22060, USA
    Mil Med 174:647-51. 2009
    ..This review summarizes work which details aspects of the pathogenesis of B. pseudomallei and mallei and discusses key scientific questions and directions for future research...
  20. pmc BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei
    Jolene R Bowers
    Translational Genomics Research Institute, Flagstaff, Arizona, United States of America
    PLoS ONE 5:e15413. 2010
    ..Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei...
  21. pmc MyD88-dependent recruitment of monocytes and dendritic cells required for protection from pulmonary Burkholderia mallei infection
    Andrew Goodyear
    Rocky Mountain RCE, CO, USA
    Infect Immun 80:110-20. 2012
    The Gram-negative bacterium Burkholderia mallei causes rapidly fatal illness in equines and humans when contracted by inhalation and also has the potential to be used as a bioweapon...
  22. ncbi Structural analysis of capsular polysaccharides expressed by Burkholderia mallei and Burkholderia pseudomallei
    Christian Heiss
    Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA
    Carbohydr Res 349:90-4. 2012
    Capsular polysaccharides (CPSs) were isolated from O-polysaccharide deficient strains of Burkholderia mallei and Burkholderia pseudomallei using a modified hot phenol/water extraction procedure...
  23. pmc Comparative genomics and an insect model rapidly identify novel virulence genes of Burkholderia mallei
    Mark A Schell
    Department of Microbiology, University of Georgia, Athens, GA 30602, USA
    J Bacteriol 190:2306-13. 2008
    Burkholderia pseudomallei and its host-adapted deletion clone Burkholderia mallei cause the potentially fatal human diseases melioidosis and glanders, respectively...
  24. ncbi Burkholderia mallei and Burkholderia pseudomallei as bioterrorism agents: national aspects of emergency preparedness
    Jacob Gilad
    CBRN Medicine Branch, Israel Defense Forces Medical Corps, Tel Hashomer, Israel
    Isr Med Assoc J 9:499-503. 2007
  25. pmc Comparison of the in vitro and in vivo susceptibilities of Burkholderia mallei to Ceftazidime and Levofloxacin
    Barbara M Judy
    Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas, USA
    BMC Microbiol 9:88. 2009
    b>Burkholderia mallei is a zoonotic Gram negative bacterium which primarily infects solipeds but can cause lethal disease in humans if left untreated...
  26. pmc Prevalence-dependent use of serological tests for diagnosing glanders in horses
    Lisa D Sprague
    OIE Reference Laboratory for Glanders, Friedrich Loeffler Institute, Naumburger Str, 96a, D 07743 Jena, Germany
    BMC Vet Res 5:32. 2009
    ..mallei whole cell antigen and a well characterised mouse monoclonal antibody with regard to their specificity and sensitivity for glanders serodiagnosis using CFT, an indirect (i) and a competitive (c) ELISA platform...
  27. ncbi Burkholderia mallei and Burkholderia pseudomallei: the causative micro-organisms of glanders and melioidosis
    Jacob Gilad
    Clinical Microbiology Laboratory, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel
    Recent Pat Antiinfect Drug Discov 2:233-41. 2007
    b>Burkholderia mallei and Burkholderia pseudomallei are the causative micro-organisms of Glanders and Melioidosis, respectively...
  28. ncbi Relationship between antigenicity and pathogenicity for Burkholderia pseudomallei and Burkholderia mallei revealed by a large panel of mouse MAbs
    Nianxiang Zou
    Department of Environmental and Infectious Disease Sciences, American Registry of Pathology, Armed Forces Institute of Pathology, Washington, DC 20306, USA
    Hybridoma (Larchmt) 27:231-40. 2008
    Burkholderia pseudomallei and Burkholderia mallei are two closely related gram-negative bacterial species classified by the CDC as category B biowarfare agents...
  29. pmc The early stage of bacterial genome-reductive evolution in the host
    Han Song
    Department of Medicine, College of Medicine, Korea University, Anam Dong, Seongbuk gu, Seoul, Korea
    PLoS Pathog 6:e1000922. 2010
    The equine-associated obligate pathogen Burkholderia mallei was developed by reductive evolution involving a substantial portion of the genome from Burkholderia pseudomallei, a free-living opportunistic pathogen...
  30. ncbi Protective cellular responses to Burkholderia mallei infection
    Caroline A Rowland
    Dstl, Biomedical Sciences, Porton Down, Salisbury SP40JQ, UK
    Microbes Infect 12:846-53. 2010
    b>Burkholderia mallei is a Gram-negative bacillus causing the disease glanders in humans...
  31. pmc Octanoyl-homoserine lactone is the cognate signal for Burkholderia mallei BmaR1-BmaI1 quorum sensing
    Breck A Duerkop
    Department of Microbiology, University of Washington School of Medicine, 1959 N E Pacific Street, Box 357242, Seattle, WA 98195 7242, USA
    J Bacteriol 189:5034-40. 2007
    ..The obligate animal pathogen Burkholderia mallei produces several acyl-HSLs, and the B...
  32. doi Glanders
    Glenda D Dvorak
    Center for Food Security and Public Health, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Am Vet Med Assoc 233:570-7. 2008
  33. pmc Humoral immune responses in a human case of glanders
    David M Waag
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Ft Detrick, Maryland, USA
    Clin Vaccine Immunol 19:814-6. 2012
    ..8-, 16-, and 4-fold increases, respectively, in specific IgA, IgG, and IgM serological titers against Burkholderia mallei. Within 14 months of infection, the titers decreased to the baseline...
  34. ncbi Development of 5' nuclease real-time PCR assays for the rapid identification of the burkholderia mallei//burkholderia pseudomallei complex
    Herbert Tomaso
    Institute of Microbiology, Federal Armed Forces, Munich, Germany
    Diagn Mol Pathol 13:247-53. 2004
    ..mallei/B. pseudomallei complex in positive blood cultures or from suspicious bacterial colonies allowing the early onset of appropriate antibiotic therapy...
  35. pmc Quorum sensing: a transcriptional regulatory system involved in the pathogenicity of Burkholderia mallei
    Ricky L Ulrich
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter St, Fort Detrick, MD 21702, USA
    Infect Immun 72:6589-96. 2004
    ..An in silico analysis of the Burkholderia mallei ATCC 23344 genome revealed that it encodes at least two luxI and four luxR homologues...
  36. pmc Molecular epidemiology of glanders, Pakistan
    Heidie Hornstra
    Northern Arizona University, Flagstaff, Arizona 86011 4073, USA
    Emerg Infect Dis 15:2036-9. 2009
    We collected epidemiologic and molecular data from Burkholderia mallei isolates from equines in Punjab, Pakistan from 1999 through 2007...
  37. ncbi Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) from culture and paraffin-embedded tissue samples
    Ralf M Hagen
    Department for Tropical Medicine at the Bernhard Nocht Institute, Bundeswehr Hospital Hamburg, Bernhard Nocht Strasse, Germany
    Int J Med Microbiol 301:585-90. 2011
    ..mallei (11), and B. thailandensis (1) strains, excluded 100% of all tested negative controls (61), and allowed demonstration of B. pseudomallei infection in a paraffin-embedded spleen tissue sample of an experimentally infected mouse...
  38. pmc Alanine racemase mutants of Burkholderia pseudomallei and Burkholderia mallei and use of alanine racemase as a non-antibiotic-based selectable marker
    Sheryl L W Zajdowicz
    Department of Microbiology, University of Colorado School of Medicine, Aurora, Colorado, United States of America
    PLoS ONE 6:e21523. 2011
    Burkholderia pseudomallei and Burkholderia mallei are category B select agents and must be studied under BSL3 containment in the United States. They are typically resistant to multiple antibiotics, and the antibiotics used to treat B...
  39. pmc Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei
    Jana M U'ren
    Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011 5640, USA
    J Clin Microbiol 43:5771-4. 2005
    ..around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates...
  40. pmc Identification of Burkholderia mallei and Burkholderia pseudomallei adhesins for human respiratory epithelial cells
    Rachel Balder
    Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA 30602, USA
    BMC Microbiol 10:250. 2010
    Burkholderia pseudomallei and Burkholderia mallei cause the diseases melioidosis and glanders, respectively...
  41. ncbi Interleukin-12 induces a Th1-like response to Burkholderia mallei and limited protection in BALB/c mice
    Kei Amemiya
    Bacteriology Division, United States Army Research Institute of Infectious Diseases, Frederick, MD 21702, USA
    Vaccine 24:1413-20. 2006
    We evaluated the effect of interleukin (IL)-12 on the immune response to Burkholderia mallei in BALB/c mice. Mice were vaccinated with non-viable B. mallei cells with or without IL-12...
  42. pmc ATP-binding cassette systems in Burkholderia pseudomallei and Burkholderia mallei
    David N Harland
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, UK
    BMC Genomics 8:83. 2007
    ..In this study, an inventory of the ABC systems of Burkholderia pseudomallei strain K96243 and Burkholderia mallei strain ATCC 23344 has been compiled using bioinformatic techniques.
  43. ncbi Antibiotic susceptibility of 65 isolates of Burkholderia pseudomallei and Burkholderia mallei to 35 antimicrobial agents
    F M Thibault
    Centre de Recherches du Service de Santé des Armées Emile Pardé, Département de Biologie des Agents Transmissibles, F 38702 La Tronche, France
    J Antimicrob Chemother 54:1134-8. 2004
    Fifty isolates of Burkholderia pseudomallei and 15 isolates of Burkholderia mallei were tested for their susceptibilities to 35 antimicrobial agents, including agents not previously tested against these bacteria.
  44. pmc Prevalence and sequence diversity of a factor required for actin-based motility in natural populations of Burkholderia species
    Chayada Sitthidet
    Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Pran Nok Road, Bangkoknoi, Bangkok 10700, Thailand
    J Clin Microbiol 46:2418-22. 2008
    ..A geographically restricted subset of B. pseudomallei isolates harbored a B. mallei-like bimA allele (12.1%), confounding a differential diagnostic test based on amplification of species-specific bimA regions...
  45. pmc Strategies toward vaccines against Burkholderia mallei and Burkholderia pseudomallei
    Sara K Bondi
    Department of Microbiology, University of Virginia, VA 22908 0734, USA
    Expert Rev Vaccines 7:1357-65. 2008
    b>Burkholderia mallei and Burkholderia pseudomallei are Gram-negative, rod-shaped bacteria, and are the causative agents of the diseases glanders and melioidosis, respectively...
  46. pmc Inactivation of [Fe-S] metalloproteins mediates nitric oxide-dependent killing of Burkholderia mallei
    Jessica Jones-Carson
    Department of Microbiology, University of Colorado Health Sciences Center, Aurora, Colorado, United States of America
    PLoS ONE 3:e1976. 2008
    ..Much remains to be known about the mechanisms by which O(2)-dependent host defenses mediate broad antimicrobial activity...
  47. ncbi Construction of a reporter system to study Burkholderia mallei type III secretion and identification of the BopA effector protein function in intracellular survival
    Gregory C Whitlock
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA
    Trans R Soc Trop Med Hyg 102:S127-33. 2008
    b>Burkholderia mallei, the aetiological agent of glanders disease, is a Gram-negative facultative intracellular bacterium. Despite numerous studies, the detailed mechanism of its pathogenesis is almost unknown...
  48. ncbi Genome-wide expression analysis of iron regulation in Burkholderia pseudomallei and Burkholderia mallei using DNA microarrays
    Apichai Tuanyok
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Alta, Canada
    FEMS Microbiol Lett 252:327-35. 2005
    ..The observations relative to the induction of specific metabolic enzymes during bacterial growth under low iron conditions warrants further experimentation...
  49. ncbi Development of mouse hybridomas for production of monoclonal antibodies specific to Burkholderia mallei and Burkholderia pseudomallei
    Shaw Huey Feng
    Department of Environmental and Infectious Disease Sciences, Armed Forces Institute of Pathology AFIP, Washington, DC, USA
    Hybridoma (Larchmt) 25:193-201. 2006
    b>Burkholderia mallei and B. pseudomallei are designated category B biothreat agents on the "select agents" list established by the NIH and CDC...
  50. pmc Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages
    John Shanks
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Infect Immun 77:1636-48. 2009
    b>Burkholderia mallei, a category B biothreat agent, is a facultative intracellular pathogen that causes the zoonotic disease glanders. The B...
  51. ncbi Experimental aerogenic Burkholderia mallei (glanders) infection in the BALB/c mouse
    M Stephen Lever
    Dstl Biomedical Sciences, Porton Down, Salisbury, Wiltshire, UK
    J Med Microbiol 52:1109-15. 2003
    The object of this study was to develop and characterize experimental Burkholderia mallei aerosol infection in BALB/c mice. Sixty-five mice were infected with 5000 [approx. 2.5 median lethal doses (MLD)] B...
  52. ncbi Human single-chain Fv antibodies against Burkholderia mallei and Burkholderia pseudomallei
    Nianxiang Zou
    Department of Environmental and Infectious Diseases, American Registry of Pathology, Armed Forces Institute of Pathology, Washington, DC 20306, USA
    Exp Biol Med (Maywood) 232:550-6. 2007
    Much effort has been devoted to the development of mouse monoclonal antibodies that react specifically with Burkholderia mallei and Burkholderia pseudomallei for diagnostic and/or therapeutic purposes...
  53. ncbi [Glanders--a comprehensive review]
    M B Wittig
    Institut for Mikrobiologie der Bundeswehr, Munchen, Deutschland
    Dtsch Tierarztl Wochenschr 113:323-30. 2006
    ..Pictures of imported endurance horses infected with glanders taken during an eradication campaign in Dubai, United Arab Emirates, in 2004 illustrate most typical clinical findings...
  54. ncbi Development of a polymerase chain reaction assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related Burkholderiaceae
    Ricky L Ulrich
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702 5011, USA
    Diagn Microbiol Infect Dis 55:37-45. 2006
    b>Burkholderia mallei and Burkholderia pseudomallei, the etiologic agents responsible for glanders and melioidosis, respectively, are genetically and phenotypically similar and are category B biothreat agents...
  55. ncbi Detection of the host immune response to Burkholderia mallei heat-shock proteins GroEL and DnaK in a glanders patient and infected mice
    Kei Amemiya
    Bacteriology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA
    Diagn Microbiol Infect Dis 59:137-47. 2007
    ..the host immune response to 2 heat-shock proteins (hsps) in a patient and mice previously infected with Burkholderia mallei. The patient was the first reported human glanders case in 50 years in the United States...
  56. pmc Monoclonal antibodies passively protect BALB/c mice against Burkholderia mallei aerosol challenge
    Sylvia R Trevino
    Bacteriology Division, U S Army Medical Research Institute of Infectious Diseases, 1425 Porter St, Fort Detrick, Frederick, MD 21702 5001, USA
    Infect Immun 74:1958-61. 2006
    ..Murine monoclonal antibodies were produced against Burkholderia mallei, the etiologic agent of glanders, and were shown to be effective in passively protecting mice against a ..
  57. ncbi Glanders: off to the races with Burkholderia mallei
    Gregory C Whitlock
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555 1070, USA
    FEMS Microbiol Lett 277:115-22. 2007
    b>Burkholderia mallei, the etiologic agent of the disease known as glanders, is primarily a disease affecting horses and is transmitted to humans by direct contact with infected animals. The use of B...
  58. pmc Characterization of clinically-attenuated Burkholderia mallei by whole genome sequencing: candidate strain for exclusion from Select Agent lists
    Steven E Schutzer
    Department of Medicine, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey, United States of America
    PLoS ONE 3:e2058. 2008
    b>Burkholderia mallei is an understudied biothreat agent responsible for glanders which can be lethal in humans and animals...
  59. pmc Mechanism and inhibition of the FabV enoyl-ACP reductase from Burkholderia mallei
    Hao Lu
    Institute for Chemical Biology and Drug Discovery, Department of Chemistry, Stony Brook University, Stony Brook, New York 11794 3400, USA
    Biochemistry 49:1281-9. 2010
    ..Chem. 283, 1308-1316]. In this work, a detailed kinetic analysis of the mechanism of the FabV enzyme from Burkholderia mallei (bmFabV) has been undertaken, which reveals that bmFabV catalyzes a sequential bi-bi mechanism with NADH ..
  60. pmc Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant
    Joanne M Stevens
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire, UK
    J Bacteriol 187:7857-62. 2005
    ..Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774...
  61. pmc Management of accidental laboratory exposure to Burkholderia pseudomallei and B. mallei
    Sharon J Peacock
    Mahidol University, Bangkok, Thailand
    Emerg Infect Dis 14:e2. 2008
    ..pseudomallei. The overall mortality rate of infected persons is 50% in northeast Thailand (35% in children) and 19% in Australia...
  62. pmc Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes
    F M Thibault
    Centre de Recherches du Service de Sante des Armees, 24 Avenue des Maquis du Gresivaudan, B P 87, F 38702 La Tronche, France
    J Clin Microbiol 42:5871-4. 2004
    ..thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species...
  63. pmc Structural flexibility in the Burkholderia mallei genome
    William C Nierman
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 101:14246-51. 2004
    The complete genome sequence of Burkholderia mallei ATCC 23344 provides insight into this highly infectious bacterium's pathogenicity and evolutionary history. B...
  64. pmc Contribution of gene loss to the pathogenic evolution of Burkholderia pseudomallei and Burkholderia mallei
    Richard A Moore
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1
    Infect Immun 72:4172-87. 2004
    ..This deletion was detected in all B. pseudomallei and Burkholderia mallei strains investigated. We cloned the B...
  65. pmc iNOS activity is critical for the clearance of Burkholderia mallei from infected RAW 264.7 murine macrophages
    Paul J Brett
    Laboratory of Zoonotic Pathogens, RTB, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
    Cell Microbiol 10:487-98. 2008
    b>Burkholderia mallei is a facultative intracellular pathogen that can cause fatal disease in animals and humans...
  66. pmc Genomic diversity of Burkholderia pseudomallei clinical isolates: subtractive hybridization reveals a Burkholderia mallei-specific prophage in B. pseudomallei 1026b
    David DeShazer
    United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702, USA
    J Bacteriol 186:3938-50. 2004
    ..phi1026b was spontaneously produced by 1026b, and it had a restricted host range, infecting only Burkholderia mallei. It possessed a noncontractile tail, an isometric head, and a linear 54,865-bp genome...
  67. ncbi Simple sequence repeat (SSR)-based gene diversity in Burkholderia pseudomallei and Burkholderia mallei
    Han Song
    Department of Medicine, College of Medicine, Korea University, Seoul, 136 705, Korea
    Mol Cells 27:237-41. 2009
    Pathogens Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm) contain a large number (> 12,000) of Simple Sequence Repeats (SSRs)...
  68. pmc Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages
    Mary N Burtnick
    Department of Microbiology and Immunology, University of South Alabama, Mobile, AL 36688, USA
    Infect Immun 78:88-99. 2010
    b>Burkholderia mallei is a facultative intracellular pathogen that causes severe disease in animals and humans...
  69. pmc The Burkholderia mallei BmaR3-BmaI3 quorum-sensing system produces and responds to N-3-hydroxy-octanoyl homoserine lactone
    Breck A Duerkop
    Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195 7242, USA
    J Bacteriol 190:5137-41. 2008
    b>Burkholderia mallei has two acyl-homoserine lactone (acyl-HSL) signal generator-receptor pairs and two additional signal receptors, all of which contribute to virulence. We show that B...
  70. pmc Type III secretion: a virulence factor delivery system essential for the pathogenicity of Burkholderia mallei
    Ricky L Ulrich
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702 5011, USA
    Infect Immun 72:1150-4. 2004
    By creating mutations in the Burkholderia mallei ATCC 23344 animal pathogen-like type III secretion system (TTSS), this study analyzes the correlation between type III secretion and the pathogenicity of ATCC 23344 in vivo...
  71. ncbi The factors shaping synonymous codon usage in the genome of Burkholderia mallei
    Sheng Zhao
    College of Life Science and Technology, Southwest University for Nationalities Chengdu 610041, China
    J Genet Genomics 34:362-72. 2007
    b>Burkholderia mallei is regarded as a potential biological weapon by the Centers for Disease Control and Prevention. In this study, the main factors shaping codon usage in the genome of B. mallei ATCC 23344 were firstly reported...
  72. ncbi Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR
    May Ann Lee
    Centre for Biomedical Sciences, Defence Medical and Environment Research Institute, DSO National Laboratories 27 Medical Drive, Singapore 117510, Singapore
    FEMS Immunol Med Microbiol 43:413-7. 2005
    ..a Gram-negative bacterium that causes melioidosis may be differentiated from closely related species of Burkholderia mallei that causes glanders and non-pathogenic species of Burkholderia thailandensis by multiplex PCR...
  73. ncbi Pulsed-field gel electrophoresis as a discriminatory typing technique for the biothreat agent burkholderia mallei
    Narisara Chantratita
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 74:345-7. 2006
    ..gel electrophoresis (PFGE) and multilocus sequence typing (MLST) was used to type 21 laboratory strains of Burkholderia mallei. We demonstrated good resolution by PFGE together with clustering of some geographically related isolates, ..
  74. ncbi Detection of the reemerging agent Burkholderia mallei in a recent outbreak of glanders in the United Arab Emirates by a newly developed fliP-based polymerase chain reaction assay
    Holger C Scholz
    Bundeswehr Institute of Microbiology, D 80937 Munich, Germany
    Diagn Microbiol Infect Dis 54:241-7. 2006
    A polymerase chain reaction (PCR) assay targeting the flagellin P (fliP)-I S407A genomic region of Burkholderia mallei was developed for the specific detection of this organism in pure cultures and clinical samples from a recent outbreak ..
  75. ncbi Using real-time PCR to specifically detect Burkholderia mallei
    Melanie P Ulrich
    Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702 5011, USA
    J Med Microbiol 55:551-9. 2006
    b>Burkholderia mallei is the causative agent of human and animal glanders and is a category B biothreat agent. Rapid diagnosis of B. mallei and immediate prophylactic treatment are essential for patient survival...
  76. ncbi Molecular identification and typing of Burkholderia pseudomallei and Burkholderia mallei: when is enough enough?
    Valery A Antonov
    Research Institute for Plague Control, Golubinskaya st 7, 400131 Volgograd, Russia
    Trans R Soc Trop Med Hyg 102:S134-9. 2008
    b>Burkholderia mallei and B. pseudomallei are highly pathogenic microorganisms for both humans and animals. Moreover, they are regarded as potential agents of bioterrorism...
  77. ncbi Genome-wide identification and mapping of variable sequences in the genomes of Burkholderia mallei and Burkholderia pseudomallei
    Alexey Fushan
    Laboratory of Structure and Functions of Human Genes, M M Shemyakin and Yu A Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16 10 Miklukho Maklaya St, 117997 GSP, Moscow V 437, Russia
    Res Microbiol 156:278-88. 2005
    b>Burkholderia mallei and Burkholderia pseudomallei, closely related Gram-negative bacteria, are the causative agents of such serious infectious diseases of humans and animals as glanders and melioidosis, respectively...
  78. ncbi Development of a 5'-nuclease real-time PCR assay targeting fliP for the rapid identification of Burkholderia mallei in clinical samples
    Herbert Tomaso
    Bundeswehr Institute of Microbiology, Munich, Germany
    Clin Chem 52:307-10. 2006
    b>Burkholderia mallei is a potential biological agent that causes glanders or farcy in solipeds, a disease notifiable to the Office International des Epizooties (OIE)...
  79. ncbi Polysaccharide microarray technology for the detection of Burkholderia pseudomallei and Burkholderia mallei antibodies
    Narayanan Parthasarathy
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA
    Diagn Microbiol Infect Dis 56:329-32. 2006
    A polysaccharide microarray platform was prepared by immobilizing Burkholderia pseudomallei and Burkholderia mallei polysaccharides. This polysaccharide array was tested with success for detecting B. pseudomallei and B...
  80. pmc The animal pathogen-like type III secretion system is required for the intracellular survival of Burkholderia mallei within J774.2 macrophages
    Wilson J Ribot
    Bacteriology Division, USAMRIID, 1425 Porter St, Fort Detrick, Frederick, MD 21702, USA
    Infect Immun 74:4349-53. 2006
    b>Burkholderia mallei is a highly infectious gram-negative pathogen and is the causative agent of human and animal glanders. By generating polar mutations (disruption of bsaQ and bsaZ) in the B...
  81. pmc Critical role of type 1 cytokines in controlling initial infection with Burkholderia mallei
    Caroline A Rowland
    Biomedical Sciences, Dstl, Porton Down, Salisbury, SP4 0JQ, United Kingdom
    Infect Immun 74:5333-40. 2006
    b>Burkholderia mallei is a gram-negative bacterium which causes the potentially fatal disease glanders in humans; however, there is little information concerning cell-mediated immunity to this pathogen...
  82. pmc Genome sequence alterations detected upon passage of Burkholderia mallei ATCC 23344 in culture and in mammalian hosts
    Claudia M Romero
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    BMC Genomics 7:228. 2006
    More than 12,000 simple sequence repeats (SSRs) have been identified in the genome of Burkholderia mallei ATCC 23344...
  83. pmc Burkholderia mallei expresses a unique lipopolysaccharide mixture that is a potent activator of human Toll-like receptor 4 complexes
    Paul J Brett
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
    Mol Microbiol 63:379-90. 2007
    b>Burkholderia mallei, the aetiologic agent of glanders, causes a variety of illnesses in animals and humans ranging from occult infections to acute fulminating septicaemias...
  84. pmc Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis
    Rachaneeporn Tiyawisutsri
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    BMC Microbiol 7:19. 2007
    The bacterial biothreat agents Burkholderia mallei and Burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that B...
  85. pmc Antibodies from patients with melioidosis recognize Burkholderia mallei but not Burkholderia thailandensis antigens in the indirect hemagglutination assay
    Rachaneeporn Tiyawisutsri
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    J Clin Microbiol 43:4872-4. 2005
    ..pseudomallei, B. mallei, and B. thailandensis antigens. We demonstrate a lack of cross-reactivity between B. pseudomallei and B. thailandensis but marked cross-reactivity between B. pseudomallei and B. mallei...
  86. ncbi Ribotyping of Burkholderia mallei isolates
    Steven P Harvey
    US Army Edgewood Chemical Biological Center, 5138 Blackhawk Road, Aberdeen Proving Ground, MD 21010 5424, USA
    FEMS Immunol Med Microbiol 44:91-7. 2005
    In this study, the subspecies differentiation of 25 isolates of Burkholderia mallei was attempted based on their ribotype polymorphisms. The isolates were from human and equine infections that occurred at various times around the world...
  87. pmc A CpG oligonucleotide can protect mice from a low aerosol challenge dose of Burkholderia mallei
    David M Waag
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter St, Fort Detrick, MD 21702 5011, USA
    Infect Immun 74:1944-8. 2006
    ..CpG ODN 7909) was found to protect BALB/c mice from lung infection or death after aerosol challenge with Burkholderia mallei. Protection was associated with enhanced levels of gamma interferon (IFN-gamma)-inducible protein 10, ..
  88. pmc Identification of Burkholderia pseudomallei and related bacteria by multiple-locus sequence typing-derived PCR and real-time PCR
    Pierre Wattiau
    Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium
    J Clin Microbiol 45:1045-8. 2007
    ..relatedness and genomic plasticity characterizing the high-threat pathogens Burkholderia pseudomallei and Burkholderia mallei render the molecular diagnosis of these species hard to guarantee with a maximal confidence level...
  89. ncbi Genomic fingerprinting of Burkholderia pseudomallei and B. mallei pathogens with DNA array based on interspecies sequence differences obtained by subtractive hybridization
    Alexey Fushan
    Laboratory of Structure and Functions of Human Genes, M M Shemyakin and Yu A Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16 10 Miklukho Maklaya St, 117997, Moscow, Russia
    Res Microbiol 157:684-92. 2006
    ..pseudomallei and B. mallei strains, as well as for their molecular diagnosis. The method reported can be easily adapted for use both with DNA arrays and DNA microarrays with fluorescent probes...
  90. pmc The type IV pilin of Burkholderia mallei is highly immunogenic but fails to protect against lethal aerosol challenge in a murine model
    Paula J Fernandes
    Division of Infectious Diseases, University of Maryland School of Medicine, 20 Penn Street, Baltimore, MD 21201, USA
    Infect Immun 75:3027-32. 2007
    b>Burkholderia mallei is the cause of glanders and a proven biological weapon. We identified and purified the type IV pilin protein of this organism to study its potential as a subunit vaccine...
  91. ncbi [Cellular fatty acid composition of the Burkholderia mallei and Burkholderia pseudomallei strains as generic feature of Burkholderia]
    Z P Vasiurenko
    Mikrobiol Z 68:33-40. 2006
    The cellular fatty acid compositions of studied two strains of Burkholderia mallei and a strain of Burkholderia pseudomallei are represented by saturated and monounsaturated straight chain fatty acids with 14-18 carbon atoms, ..
  92. ncbi Tropical medicine: melioidosis? Never heard of it
    Peter Aldhous
    Nature 434:692-3. 2005
  93. ncbi Natural products from isnA-containing biosynthetic gene clusters recovered from the genomes of cultured and uncultured bacteria
    Sean F Brady
    Laboratory of Genetically Encoded Small Molecules, The Rockefeller University, New York, New York 10021, USA
    J Am Chem Soc 129:12102-3. 2007
  94. ncbi Brothers in arms
    Nicholas Thomson
    Nat Rev Microbiol 3:100-1. 2005
  95. ncbi Serodiagnosis of Burkholderia mallei infections in horses: state-of-the-art and perspectives
    H Neubauer
    Institut fur Mikrobiologie der Bundeswehr, Neuherbergstr 11, 80937 München, Germany
    J Vet Med B Infect Dis Vet Public Health 52:201-5. 2005
    b>Burkholderia mallei causes glanders or farcy in solipeds, a disease that must be reported to the OIE (Office International des Epizooties, Paris, France). The number of reported outbreaks has increased steadily during the last decade...
  96. ncbi Comparison of efficacy of ciprofloxacin and doxycycline against experimental melioidosis and glanders
    P Russell
    CBD Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    J Antimicrob Chemother 45:813-8. 2000
    Melioidosis and glanders are caused by the closely related species Burkholderia pseudomallei and Burkholderia mallei, respectively. Whereas melioidosis is a significant cause of morbidity in south-east Asia, glanders is extremely rare...
  97. ncbi DNA assays for detection, identification, and individualization of select agent microorganisms
    Susan W Jones
    Armed Forces Institute of Pathology, Department of Microbiology, 6825 16th Street NW, Washington, DC 20306, USA
    Croat Med J 46:522-9. 2005
    ..identification, and individualization of Bacillus anthracis, Yersinia pestis, Francisella tularensis, Burkholderia mallei, and Brucella abortus...
  98. pmc Nonviable Burkholderia mallei induces a mixed Th1- and Th2-like cytokine response in BALB/c mice
    Kei Amemiya
    Pathogenesis and Immunology Branch, Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702, USA
    Infect Immun 70:2319-25. 2002
    Nonviable cell preparations of Burkholderia mallei, the causative agent of glanders, were evaluated as potential vaccine candidates in a BALB/c murine model. Three different B...
  99. ncbi Antigenic relatedness between Burkholderia pseudomallei and Burkholderia mallei
    Narisara Anuntagool
    Laboratory of Immunology, Chulabhorn Research Institute, Bangkok, Thailand
    Microbiol Immunol 46:143-50. 2002
    Burkholderia pseudomallei and Burkholderia mallei are causative agents of distinct diseases, namely, melioidosis and glanders, respectively...
  100. pmc Engineering of tellurite-resistant genetic tools for single-copy chromosomal analysis of Burkholderia spp. and characterization of the Burkholderia thailandensis betBA operon
    Yun Kang
    Department of Microbiology, University of Hawaii at Manoa, Honolulu, 96822, USA
    Appl Environ Microbiol 75:4015-27. 2009
    ..clinically important antibiotics is prohibited for the bioterrorism agents Burkholderia pseudomallei and Burkholderia mallei, necessitating the development of additional nonantibiotic-based genetic tools...
  101. pmc Glyphosate resistance as a novel select-agent-compliant, non-antibiotic-selectable marker in chromosomal mutagenesis of the essential genes asd and dapB of Burkholderia pseudomallei
    Michael H Norris
    Department of Microbiology, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA
    Appl Environ Microbiol 75:6062-75. 2009
    Genetic manipulation of the category B select agents Burkholderia pseudomallei and Burkholderia mallei has been stifled due to the lack of compliant selectable markers...

Research Grants35

  1. Optimization of Neoglycoside Antibiotics for Nosocomial Pathogens and Select Agen
    Arnold Louie; Fiscal Year: 2013
    ..to provide new products for the therapy of Select Agents, such as Plague, Anthrax and pathogens such as Burkholderia mallei and pseudomallei...
  2. Translational Immunology Research and Accelerated [vaccine] Development (TRIAD)
    ANNE SEARLS DEGROOT; Fiscal Year: 2013
    ..TRIAD has selected Category A pathogens F. tularensis, Category B agents Burkholderia pseudomallei and Burkholderia mallei, and emerging infectious diseases (HCV, H. pylori, tick borne diseases) as the focal point of this proposal...
  3. Regulatory Circuits Controlling Development of Dormant Microbial Cysts
    CARL EUGENE BAUER; Fiscal Year: 2013
    ..medicae, Sinorhizobium meliloti, Sinorhizobium fredii, Delftia acidovorans (Comamonas acidovorans), Burkholderia mallei (Pseudomonas mallei))...
  4. Development and Testing of Novel Glanders Vaccine Candidates
    Paul J Brett; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Burkholderia mallei, the etiologic agent of glanders, cause severe disease in humans and animals and are a potential agent of biological warfare and terrorism...
  5. Nanoscale Optical Fiber Biosensor Assays for Detection of Category A and B Select
    Thomas J Inzana; Fiscal Year: 2010
    ..optical fiber biosensors for the diagnosis of the Category A and B select agents Francisella tularensis, Burkholderia mallei, and B...
  6. Development of a Novel Lead Series Against Category A &B Bacterial Pathogens
    Terry L Bowlin; Fiscal Year: 2013
    ..species, including a number of Category A and B agents such as Yersinia pestis, Francisella tularensis, Burkholderia mallei and pseudomallei. These compounds are also active against ciprofloxacin-resistant Bacillus anthracis (MIC 0...
  7. Non-invasive Optical Imaging of Select Agent Bacteria in Non-human Primates
    Frederick D Quinn; Fiscal Year: 2010
    ..This is particularly true for the candidate bioterrorism agents, Burkholderia. pseudomallei (Bp) and Burkholderia mallei (Bm), the causative agents of human melioidosis and glanders...
  8. Analysis of a Burkholderia mallei Virulence-Associated Type VI Secretion System
    Paul J Brett; Fiscal Year: 2011
    PROJECT SUMMARY/ABSTRACT Burkholderia mallei, the etiologic agent of glanders, causes severe disease in humans and animals and is a potential agent of biological warfare and terrorism...
  9. IMPDH-targeted antibiotics for select agents
    LIZBETH K HEDSTROM; Fiscal Year: 2013
    ..facultative intracellular), Listeria monocytogenes (Gram- positive facultative intracellular), Burkholderia mallei/pseudomallei (Gram-negative facultative intracellular), Staphylococcus aureus (Gram-positive) and ..
  10. Burkholderia: Characterizing Lung Innate Immunity
    Tonyia D Eaves Pyles; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: Burkholderia mallei and Burholderia pseudomallei can be used as a bioweapon against the public...
  11. Novel Molecular Recognition Reagents for Detection of Pathogenic Burkholderia
    KATHERINE ANNE BROWN; Fiscal Year: 2010
    ..are to develop an efficient novel assay methodology for the detection of the category B biothreat agents, Burkholderia mallei (the causative agent of glanders) and Burkholderia pseudomallei (the causative agent of melioidosis)...
  12. Hexameric PBGS as a Bioterrorism Defense
    Eileen Jaffe; Fiscal Year: 2006
    ..directed against the NIAID priority pathogens Burkholderia pseudomallei, Brucella mefitensis, Burkholderia mallei, Rickettsia prowazekii, Vibrio cholerae, and Yersinia enterocolitica...
  13. Quorum Sensing in Burkholderia mallei
    Josephine R Chandler; Fiscal Year: 2010
    ..QS is important for virulence in many organisms, including the category B bioagent Burkholderia mallei, the causative agent of glanders. Disruption of one of the B...
  14. Type IV Pilins as Vaccines against Bioterrorism Threats
    Michael Donnenberg; Fiscal Year: 2004
    ..b>Burkholderia mallei and B...
  15. Vaccine discovery for Burkholderia via protection screen
    JEFFREY NORDSTROM; Fiscal Year: 2003
    ..Even if quickly diagnosed as Burkholderia mallei and treated, antibiotics do not control infections well...
  16. Identification of B. pseudomallei & B. mallei adhesins
    ERIC LAFONTAINE; Fiscal Year: 2006
    Burkholderia pseudomallei and Burkholderia mallei cause serious and often fatal infections that generally occur through the aerosol route and which manifest as severe pneumonia and highly fatal bacteremia...
  17. SEQUENCING THE GENOME BURKHOLDERIA MALLEI
    William Nierman; Fiscal Year: 2001
    ..They are cheap, easy to make, and easy to use. Burkholderia mallei, one of the organisms used by the Soviets in their bioweapons program, possesses properties which ..
  18. Novel genetic tools for Burkholderia mallei and other bacterial Select Agents
    Randall Holmes; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  19. Extracellular Proteins in Virulence of B. (pseudo)mallei
    Mark Schell; Fiscal Year: 2007
    ..DESCRIPTION (provided by applicant): Burkholderia pseudomallei (Bp), and its diminutive deletion clone Burkholderia mallei (Bm) cause fatal human diseases...
  20. Discovery of new anti-bacteremia vaccines for anthrax
    Kathryn Sykes; Fiscal Year: 2005
    ..These will be ready for preclinical trials. We should also have in place a high-throughput antigen discovery system that could be applied to other biothreat and emerging pathogens. ..
  21. Plague vaccine candidates by a functional genomic screen
    Kathryn Sykes; Fiscal Year: 2002
    ..abstract_text> ..
  22. Host-Pathogen Interactions in Invasive Aspergillosis
    WILLIAM CHARLES NIERMAN; Fiscal Year: 2010
    ..necessary to maintain protective immunity against the invading fungus. This hithertn inaccessible ... in order to >.E =.m m'- N'3 ..' 'C3 _.n '-' a.. m._ o'< m- ..
  23. Adherence mechanisms of Moraxella catarrhalis
    ERIC LAFONTAINE; Fiscal Year: 2007
    ..catarrhalis adhesins to determine whether they elicit the production of biologically relevant antibodies. Our studies will have direct applications in vaccine as well as antimicrobial development. ..
  24. Microarray Expression Analysis of Burkholderia Mallei
    William Nierman; Fiscal Year: 2003
    b>Burkholderia mallei, one of the organisms used by the Soviets in their bioweapons program, possesses properties that contribute to its potential as a biological weapon...
  25. The Transcriptome of Pathogenic Aspergillus Fumigatus
    William Nierman; Fiscal Year: 2003
    ..abstract_text> ..
  26. Non-antiobiotic resistance markers for bacteria
    Herbert Schweizer; Fiscal Year: 2006
    ..of the newly constructed markers for priority pathogen research will be tested by using the examples of Burkholderia mallei, B. pseudomallei, Francisella tulerensis and Yersinia pestis...
  27. ASSAY TO DETECT ACUTE ACTIVE LYME DISEASE
    Steven Schutzer; Fiscal Year: 2005
    ..It can also detect infection early than free antibody assays. Therefore this assay can fulfill a great need in Lyme disease diagnosis and has high market potential. ..
  28. IgA inducing peptide
    D Estes; Fiscal Year: 2005
    ..In addition, the proposed studies will begin to examine determine the potential relationship between IGIP and clinical abnormality in IgA production. ..
  29. Complete Proteome of Cerebrospinal Fluid
    Steven Schutzer; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  30. PSEUDOMONAS AUTOINDUCER BIOSYNTHESIS AND DRUG DISCOVERY
    Herbert Schweizer; Fiscal Year: 2001
    ..These studies should provide a better understanding of the quorum sensing pathways and lead to the identification of novel anti microbial targets and antimicrobials effective against gram-negative pathogens. ..
  31. Assay to detect early neurologic Lyme disease
    Steven Schutzer; Fiscal Year: 2003
    ..BbIC Ab and Ag will be identified. Focus will be towards on specific and in vivo expressed Bb Ags. Results will be statistically analyzed. Phase 2 will be designed for broadscale usage and refinement. ..
  32. Regulation of Burkholderia mallei virulence genes
    William Nierman; Fiscal Year: 2005
    DESCRIPTION (provided by the applicant): Burkholderia mallei, the etiologic agent of glanders, is an obligate parasite of horses, mules and donkeys, and is classified as a Category B agent by the Centers for Disease Control and ..
  33. Genetic Tools for Pathogenic Bacteria
    Herbert Schweizer; Fiscal Year: 2005
    ....
  34. Regulation and function of Pseudomonas drug efflux pumps
    Herbert Schweizer; Fiscal Year: 2006
    ..Aim 3: Probe other, perhaps global regulators of efflux operon expression, specifically MexS and PA4878. ..
  35. Detection of Virulent Burkholderia mallei
    Steven Schutzer; Fiscal Year: 2006
    DESCRIPTION (provided by investigator): Our objective is to develop discriminating diagnostic assays to Burkholderia mallei, the cause of glanders. It is an understudied biothreat agent that has been used by our adversaries...