Genomes and Genes
Summary: A species of gram-negative, aerobic bacteria that causes formation of root nodules on some, but not all, types of sweet clover, MEDICAGO SATIVA, and fenugreek.
Publications266 found, 100 shown here
- How rhizobial symbionts invade plants: the Sinorhizobium-Medicago modelKathryn M Jones
Department of Biology, Massachusetts Institute of Technology, Building 68, Room 633, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
Nat Rev Microbiol 5:619-33. 2007..we review recent discoveries about the mutual recognition process that allows the model rhizobial symbiont Sinorhizobium meliloti to invade and differentiate inside its host plant alfalfa (Medicago sativa) and the model host plant ..
- A genome-wide survey of sRNAs in the symbiotic nitrogen-fixing alpha-proteobacterium Sinorhizobium melilotiJan Philip Schlüter
Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany
BMC Genomics 11:245. 2010..This study reports on a comprehensive screen for sRNAs in the symbiotic nitrogen-fixing alpha-proteobacterium Sinorhizobium meliloti applying deep sequencing of cDNAs and microarray hybridizations.
- A gene expression atlas of the model legume Medicago truncatulaVagner A Benedito
Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
Plant J 55:504-13. 2008..The data presented here represent an unparalleled resource for legume functional genomics, which will accelerate discoveries in legume biology...
- Plant peptides govern terminal differentiation of bacteria in symbiosisWillem Van de Velde
Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette Cedex, France
Science 327:1122-6. 2010..Because NCRs resemble antimicrobial peptides, our findings reveal a previously unknown innovation of the host plant, which adopts effectors of the innate immune system for symbiosis to manipulate the cell fate of endosymbiotic bacteria...
- The genome of the natural genetic engineer Agrobacterium tumefaciens C58D W Wood
Department of Microbiology, University of Washington, 1959 NE Pacific Street, Box 357242, Seattle, WA 98195, USA
Science 294:2317-23. 2001..tumefaciens and the plant symbiont Sinorhizobium meliloti suggest a recent evolutionary divergence...
- A remorin protein interacts with symbiotic receptors and regulates bacterial infectionBenoit Lefebvre
Laboratoire des Interactions Plantes Micro organismes, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, 31326 Castanet Tolosan Cedex, France
Proc Natl Acad Sci U S A 107:2343-8. 2010..We used root nodule symbiosis between Medicago truncatula and Sinorhizobium meliloti to study the roles of a remorin that is specifically induced during nodulation...
- A highly conserved protein of unknown function in Sinorhizobium meliloti affects sRNA regulation similar to HfqShree P Pandey
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
Nucleic Acids Res 39:4691-708. 2011..Indeed, eliminating SMc01113/YbeY expression in Sinorhizobium meliloti produces symbiotic and physiological phenotypes strikingly similar to those of the hfq mutant...
- Proteomic alterations explain phenotypic changes in Sinorhizobium meliloti lacking the RNA chaperone HfqLise Barra-Bily
Interactions Cellulaires et Moleculaires, DUALS, CNRS UMR 6026, Universite de Rennes I, Campus de Beaulieu, 35042 Rennes Cedex, France
J Bacteriol 192:1719-29. 2010The ubiquitous bacterial RNA-binding protein Hfq is involved in stress resistance and pathogenicity. In Sinorhizobium meliloti, Hfq is essential for the establishment of symbiosis with Medicago sativa and for nitrogen fixation...
- Global transcriptional analysis of the phosphate starvation response in Sinorhizobium meliloti strains 1021 and 2011E Krol
Lehrstuhl fur Genetik, Fakultat fur Biologie, Universitat Bielefeld, Postfach 100131, 33501, Bielefeld, Germany
Mol Genet Genomics 272:1-17. 2004..to phosphate starvation was analysed at the transcriptional level in two closely related strains of Sinorhizobium meliloti, Rm1021 and Rm2011...
- The Sinorhizobium meliloti RNA chaperone Hfq influences central carbon metabolism and the symbiotic interaction with alfalfaOmar Torres-Quesada
Grupo de Ecología Genética de la Rizosfera, Estacion Experimental del Zaidin, CSIC, Profesor Albareda, 1, 18008 Granada, Spain
BMC Microbiol 10:71. 2010..b>Sinorhizobium meliloti is a model soil bacterium known for its ability to establish a beneficial nitrogen-fixing intracellular ..
- A novel family in Medicago truncatula consisting of more than 300 nodule-specific genes coding for small, secreted polypeptides with conserved cysteine motifsPeter Mergaert
Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, Avenue de la Terrasse, 91198 Gif sur Yvette, France
Plant Physiol 132:161-73. 2003..Potential functions of the NCR polypeptides in cell-to-cell signaling and creation of a defense system are discussed...
- Crystal structure of the flavoprotein ArsH from Sinorhizobium melilotiJun Ye
Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA
FEBS Lett 581:3996-4000. 2007Purified ArsH from Sinorhizobium meliloti exhibits NADPH:FMN-dependent reduction of molecular O2 to hydrogen peroxide and catalyzes reduction of azo dyes. The structure of ArsH was determined at 1.8A resolution...
- Expression profiling in Medicago truncatula identifies more than 750 genes differentially expressed during nodulation, including many potential regulators of the symbiotic programFikri El Yahyaoui
Laboratoire des Interactions Plantes Micro organismes, Institut National de la Recherche Agronomique Centre National de la Recherche Scientifique, 31326 Castanet Tolosan Cedex, France
Plant Physiol 136:3159-76. 2004..the symbiotic interaction between the model legume Medicago truncatula and the nitrogen-fixing bacterium Sinorhizobium meliloti. Macro- and microarrays containing about 6,000 probes were generated on the basis of three cDNA libraries ..
- The composite genome of the legume symbiont Sinorhizobium melilotiF Galibert
UMR6061-CNRS, , , , F-35043 Rennes Cedex, France
Science 293:668-72. 2001..We present here the annotated DNA sequence of the alpha-proteobacterium Sinorhizobium meliloti, the symbiont of alfalfa. The tripartite 6.7-megabase (Mb) genome comprises a 3.65-Mb chromosome, and 1...
- The Medicago truncatula lysin [corrected] motif-receptor-like kinase gene family includes NFP and new nodule-expressed genesJean Francois Arrighi
Laboratoire des Interactions Plantes Microorganismes, Institut National de la Recherche Agronomique Centre National de la Recherche Scientifique, Unité Mixte de Recherche 441 2594, 31326 Castanet Tolosan, France
Plant Physiol 142:265-79. 2006..Most of the newly identified genes were found to be expressed in roots and nodules, suggesting this class of receptors may be more extensively involved in nodulation than was previously known...
- Use of RmInt1, a group IIB intron lacking the intron-encoded protein endonuclease domain, in gene targetingFernando M García-Rodríguez
Grupo de Ecología Genética, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Científica, C Profesor Albareda 1, 18008 Granada, Spain
Appl Environ Microbiol 77:854-61. 2011..subclass IIB3/D intron with an IEP lacking D and En domains, is highly active in retrohoming in its host, Sinorhizobium meliloti. We found that RmInt1 was also mobile in E...
- Auxin and nitric oxide control indeterminate nodule formationYoury Pii
Dipartimento Scientifico Tecnologico, University of Verona, Verona, Italy
BMC Plant Biol 7:21. 2007..Both plants and rhizobia synthesise auxin. We have addressed the effects of bacterial auxin (IAA) on nodulation by using Sinorhizobium meliloti and Rhizobium leguminosarum bacteria genetically engineered for increased auxin synthesis.
- Importance of trehalose biosynthesis for Sinorhizobium meliloti Osmotolerance and nodulation of Alfalfa rootsAna Domínguez-Ferreras
Departamento de Microbiologia del Suelo y Sistemas Simbioticos, Estacion Experimental del Zaidin, CSIC, Granada, Spain
J Bacteriol 191:7490-9. 2009..In this study, we have investigated the role of endogenous trehalose synthesis in the osmotolerance of Sinorhizobium meliloti. Genes coding for three possible trehalose synthesis pathways are present in the genome of S...
- Dual control of Sinorhizobium meliloti RpoE2 sigma factor activity by two PhyR-type two-component response regulatorsBénédicte Bastiat
Laboratoire des Interactions Plantes Microorganismes LIPM, UMR 441 2594 INRA CNRS BP52627, 31326 Castanet Tolosan Cedex, France
J Bacteriol 192:2255-65. 2010RpoE2 is an extracytoplasmic function (ECF) sigma factor involved in the general stress response of Sinorhizobium meliloti, the nitrogen-fixing symbiont of the legume plant alfalfa...
- A nodule-specific protein secretory pathway required for nitrogen-fixing symbiosisDong Wang
Department of Biology, Stanford University, Stanford, CA 94305, USA
Science 327:1126-9. 2010The nitrogen-fixing symbiosis between Sinorhizobium meliloti and its leguminous host plant Medicago truncatula occurs in a specialized root organ called the nodule...
- The low-molecular-weight fraction of exopolysaccharide II from Sinorhizobium meliloti is a crucial determinant of biofilm formationLuciana V Rinaudi
Departamento de Biologia Molecular, Universidad Nacional de Rio Cuarto, 5800 Rio Cuarto, Cordoba, Argentina
J Bacteriol 191:7216-24. 2009b>Sinorhizobium meliloti is a soil bacterium that elicits the formation of root organs called nodules on its host plant, Medicago sativa...
- The Rhizobium meliloti putA gene: its role in the establishment of the symbiotic interaction with alfalfaJ I Jimenez-Zurdo
Departamento de Microbiologia del Suelo y Sistemas Simbioticos, Estacion Experimental del Zaidin, CSIC, Granada, Spain
Mol Microbiol 23:85-93. 1997..Furthermore, we show that the PutA- phenotype leads to a significant reduction of alfalfa root colonization by R. meliloti...
- (Homo)glutathione depletion modulates host gene expression during the symbiotic interaction between Medicago truncatula and Sinorhizobium melilotiChiara Pucciariello
Interactions Biotiques et Santé Végétale, Unite Mixte de Recherche, Institut National de la Recherche Agronomique 1301, Centre National de la Recherche Scientifique 6243, Universite de Nice Sophia Antipolis, 06903 Sophia Antipolis Cedex, France
Plant Physiol 151:1186-96. 2009..truncatula. In this study, the plant transcriptomic response to Sinorhizobium meliloti infection under (h)GSH depletion was investigated using cDNA-amplified fragment length polymorphism ..
- The role of sigma factor RpoH1 in the pH stress response of Sinorhizobium melilotiDaniella K C de Lucena
Lehrstuhl fur Genetik, Fakultat fur Biologie, Universitat Bielefeld, Bielefeld, Germany
BMC Microbiol 10:265. 2010..The purpose of this study was to gain detailed insight into the participation of sigma factors in the complex stress response system of S. meliloti 1021 using pH stress as an effector...
- Sinorhizobium meliloti metabolism in the root nodule: a proteomic perspectiveMichael A Djordjevic
Australian Research Council Centre of Excellence for Integrative Legume Research, Genomic Interactions Group, Research School of Biological Sciences, Australian National University, GPO Box 475, Canberra, ACT 2601, Australia
Proteomics 4:1859-72. 2004The proteome of the model symbiotic bacterium, Sinorhizobium meliloti was examined to determine the enzymatic reactions and cell processes that occur when S. meliloti occupies the root nodules of Medicago truncatula and Melilotus alba...
- Regulation and properties of PstSCAB, a high-affinity, high-velocity phosphate transport system of Sinorhizobium melilotiZe Chun Yuan
Center for Environmental Genomics, Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1
J Bacteriol 188:1089-102. 2006The properties and regulation of the pstSCAB-encoded Pi uptake system from the alfalfa symbiont Sinorhizobium meliloti are reported...
- Functional characterization of the Sinorhizobium meliloti acetate metabolism genes aceA, SMc00767, and glcBJ A Ramírez-Trujillo
Departamento de Microbiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Av Universidad 2001, Cuernavaca, Morelos 62210, Mexico
J Bacteriol 189:5875-84. 2007..open reading frame (SMc00767) located downstream of aceA were isolated and functionally characterized in Sinorhizobium meliloti. Independent and double interposon mutants of each gene were constructed, and the corresponding phenotypes ..
- The model legume Medicago truncatula A17 is poorly matched for N2 fixation with the sequenced microsymbiont Sinorhizobium meliloti 1021Jason J Terpolilli
Centre for Rhizobium Studies, School of Biological Sciences and Biotechnology, Murdoch University, South St, Murdoch, WA 6150, Australia
New Phytol 179:62-6. 2008..is currently being sequenced as a model legume, complementing the sequenced root nodule bacterial strain Sinorhizobium meliloti 1021 (Sm1021). In this study, the effectiveness of the Sm1021-M...
- Proteomic analysis of wild-type Sinorhizobium meliloti responses to N-acyl homoserine lactone quorum-sensing signals and the transition to stationary phaseHancai Chen
Genomic Interactions Group, Research School for Biological Sciences, Australian National University, Canberra, Australia
J Bacteriol 185:5029-36. 2003Proteome analysis revealed that two long-chain N-acyl homoserine lactones (AHLs) produced by Sinorhizobium meliloti 1021 induced significant differences in the accumulation of more than 100 polypeptides in early-log-phase cultures of the ..
- ppGpp in Sinorhizobium meliloti: biosynthesis in response to sudden nutritional downshifts and modulation of the transcriptomeElizaveta Krol
Faculty of Biology and Center for Systems Biology, University of Freiburg, Schänzlestr 1, 79104 Freiburg, Germany
Mol Microbiol 81:1233-54. 2011b>Sinorhizobium meliloti Rm2011 responds to sudden shifts to nitrogen or carbon starvation conditions by an accumulation of the stringent response alarmone ppGpp and remodelling of the transcriptome...
- Quorum sensing controls exopolysaccharide production in Sinorhizobium melilotiMelanie M Marketon
Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX 75083 0688, USA
J Bacteriol 185:325-31. 2003b>Sinorhizobium meliloti is a soil bacterium capable of invading and establishing a symbiotic relationship with alfalfa plants. This invasion process requires the synthesis, by S...
- EFD Is an ERF transcription factor involved in the control of nodule number and differentiation in Medicago truncatulaTatiana Vernié
Laboratoire des Interactions Plantes Micro organismes, Unite Mixte de Recherche, Centre National de la Recherche Scientifique Institut National de la Recherche Agronomique 2594 441, F 31320 Castanet Tolosan, France
Plant Cell 20:2696-713. 2008..Our work thus reveals a key regulator linking early and late stages of nodulation and suggests that the regulation of the cytokinin pathway is important both for nodule initiation and development...
- Investigations of Rhizobium biofilm formationNancy A Fujishige
Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, USA
FEMS Microbiol Ecol 56:195-206. 2006..We developed conditions for analyzing the ability of two fast-growing rhizobia, Sinorhizobium meliloti and Rhizobium leguminosarum bv...
- Mapping the Sinorhizobium meliloti 1021 solute-binding protein-dependent transportomeT H Mauchline
School of Biological Sciences, University of Reading, Reading RG6 6AJ, United Kingdom
Proc Natl Acad Sci U S A 103:17933-8. 2006..to nearly all ATP-binding cassette (ABC) and tripartite ATP-independent periplasmic (TRAP) transporters of Sinorhizobium meliloti. In total, specific inducers were identified for 76 transport systems, amounting to approximately 47% of ..
- Sinorhizobium meliloti, a bacterium lacking the autoinducer-2 (AI-2) synthase, responds to AI-2 supplied by other bacteriaCatarina S Pereira
Bacterial Signaling Laboratory, Instituto Gulbenkian de Ciencia, Oeiras, Portugal
Mol Microbiol 70:1223-35. 2008..Here, we show that Sinorhizobium meliloti, a plant symbiont lacking the gene for the AI-2 synthase, while not capable of producing AI-2 can ..
- Role of the Sinorhizobium meliloti global regulator Hfq in gene regulation and symbiosisMengsheng Gao
Soil and Water Science Department, Cancer and Genetics Research Complex, Room 330E, University of Florida Institute of Food and Agricultural Sciences, Gainesville 32610, USA
Mol Plant Microbe Interact 23:355-65. 2010..To begin understanding the role of Hfq in the Sinorhizobium meliloti-Medicago truncatula nitrogen-fixing symbiosis, we defined free-living and symbiotic phenotypes of an hfq ..
- The response of carbon metabolism and antioxidant defenses of alfalfa nodules to drought stress and to the subsequent recovery of plantsLoreto Naya
Departamento de Nutrición Vegetal, Estacion Experimental de Aula Dei, Consejo Superior de Investigaciones Cientificas, 50080 Zaragoza, Spain
Plant Physiol 144:1104-14. 2007..We conclude that a limitation in metabolic capacity of bacteroids and oxidative damage of cellular components are contributing factors to the inhibition of N(2)ase activity in alfalfa nodules...
- Transcription reprogramming during root nodule development in Medicago truncatulaSandra Moreau
Laboratoire des Interactions Plantes Micro organismes, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Castanet Tolosan, France
PLoS ONE 6:e16463. 2011..have been used to study the transcriptome of root nodules induced by either wild type or mutant strains of Sinorhizobium meliloti. In this way we have defined eight major activation patterns in nodules and identified associated ..
- Nitrogen fixation mutants of Medicago truncatula fail to support plant and bacterial symbiotic gene expressionColby G Starker
Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
Plant Physiol 140:671-80. 2006..To monitor bacterial status in mutant nodules, we assayed Sinorhizobium meliloti symbiosis gene promoters (nodF, exoY, bacA, and nifH) in the defective in nitrogen fixation mutants...
- Sinorhizobium meliloti differentiation during symbiosis with alfalfa: a transcriptomic dissectionDelphine Capela
Laboratoire des Interactions Plantes Microorganismes, UMR INRA CNRS 441 2594, BP52627, 31326 Castanet Tolosan, France
Mol Plant Microbe Interact 19:363-72. 2006b>Sinorhizobium meliloti is a soil bacterium able to induce the formation of nodules on the root of specific legumes, including alfalfa (Medicago sativa)...
- Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associationsA Boisson-Dernier
, INRA-CNRS UMR215, Castanet-Tolosan, France
Mol Plant Microbe Interact 14:695-700. 2001..morphology to normal roots and can be nodulated successfully by their nitrogen-fixing symbiotic partner, Sinorhizobium meliloti. Furthermore, spatiotemporal expression of the Nod factor-responsive reporter pMtENOD11-gusA in hairy root ..
- Analysis of infection thread development using Gfp- and DsRed-expressing Sinorhizobium melilotiDaniel J Gage
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269, USA
J Bacteriol 184:7042-6. 2002b>Sinorhizobium meliloti growth inside infection threads was monitored after inoculation of alfalfa with red- or green-tagged bacteria. Most threads were populated with single bacterial types...
- Extension of the Rhizobium meliloti succinoglycan biosynthesis gene cluster: identification of the exsA gene encoding an ABC transporter protein, and the exsB gene which probably codes for a regulator of succinoglycan biosynthesisA Becker
Lehrstuhl fur Genetik, Fakultat fur Biologie, Universitat Bielefeld, Federal Republic of Germany
Mol Gen Genet 249:487-97. 1995..It was demonstrated that this influence is not due to transcriptional regulation of the exo genes by the exsB gene product. By plasmid integration it was shown that exsA and exsB represent monocistronic transcription units...
- sinI- and expR-dependent quorum sensing in Sinorhizobium melilotiMengsheng Gao
Department of Horticulture and Crop Science, Ohio State University, Columbus, 43210, USA
J Bacteriol 187:7931-44. 2005Quorum sensing (QS) in Sinorhizobium meliloti, the N-fixing bacterial symbiont of Medicago host plants, involves at least half a dozen different N-acyl homoserine lactone (AHL) signals and perhaps an equal number of AHL receptors...
- The twin arginine transport system appears to be essential for viability in Sinorhizobium melilotiBrad S Pickering
Department of Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada
J Bacteriol 192:5173-80. 2010..Since the annotation of Sinorhizobium meliloti Rm1021 genome suggests that there may be up to 94 putative Tat substrates, we hypothesized that ..
- Sinorhizobium meliloti plasmid pRm1132f replicates by a rolling-circle mechanismL R Barran
Soils and Crops Research and Development Centre, Agriculture and Agri Food Canada, Sainte Foy, Quebec G1V 2J3, Canada
J Bacteriol 183:2704-8. 2001pRm1132f isolated from Sinorhizobium meliloti is a group III rolling-circle-replicating (RCR) plasmid. At least seven of eight open reading frames in the nucleotide sequence represented coding regions...
- Cell cycle regulation in the course of nodule organogenesis in MedicagoF Foucher
, UPR40, Centre National de la Recherche Scientifique, Gif sur Yvette, France
Plant Mol Biol 43:773-86. 2000..This review summarizes the present knowledge on cell cycle reactivation and meristem formation in response to Nod factors and reports on a novel plant cell cycle regulator that can switch mitotic cycles to differentiation programmes...
- Population genomics of the facultatively mutualistic bacteria Sinorhizobium meliloti and S. medicaeBrendan Epstein
Department of Plant Biology, University of Minnesota, St Paul, Minnesota, United States of America
PLoS Genet 8:e1002868. 2012..Based upon available functional information, several genes identified as targets of selection are likely to alter the symbiosis with the host plant, making them attractive targets for further functional characterization...
- Sinorhizobium meliloti ExoR is the target of periplasmic proteolysisHai Yang Lu
Biological Sciences Department, Lehman College, The City University of New York, Bronx, New York, USA
J Bacteriol 194:4029-40. 2012b>Sinorhizobium meliloti ExoR regulates the production of succinoglycan and flagella through the ExoS/ChvI two-component regulatory system. ExoR has been proposed to inhibit the ExoS sensor through direct interaction in the periplasm...
- EPS II-dependent autoaggregation of Sinorhizobium meliloti planktonic cellsFernando G Sorroche
Departamento de Biologia Molecular, Universidad Nacional de Rio Cuarto, Rio Cuarto, Cordoba, Argentina
Curr Microbiol 61:465-70. 2010Planktonic cells of Sinorhizobium meliloti, a Gram-negative symbiotic bacterium, display autoaggregation under static conditions. ExpR is a LuxR-type regulator that controls many functions in S...
- Multiple genetic controls on Rhizobium meliloti syrA, a regulator of exopolysaccharide abundanceM J Barnett
Department of Biological Sciences, Stanford University, California 94305, USA
Genetics 148:19-32. 1998..Instead, expression of the nitrogen fixation (nifHDKE) genes upstream of syrA plays a role. Specific and distinct sets of genetic controls may operate at different times during nodule invasion...
- Six nonnodulating plant mutants defective for Nod factor-induced transcriptional changes associated with the legume-rhizobia symbiosisRaka M Mitra
Department of Biological Sciences, 371 Serra Mall, Stanford University, CA 94305 5020, USA
Proc Natl Acad Sci U S A 101:10217-22. 2004..We identified 46 sequences that are differentially expressed in plants exposed for 24 h to WT Sinorhizobium meliloti or to the invasion defective S. meliloti mutant, exoA...
- MotE serves as a new chaperone specific for the periplasmic motility protein, MotC, in Sinorhizobium melilotiElke Eggenhofer
Lehrstuhl fur Genetik, Universitat Regensburg, D 93040 Regensburg, Germany
Mol Microbiol 52:701-12. 2004The flagella of Sinorhizobium meliloti rotate solely clockwise and vary their rotary speed to provoke changes in the swimming path...
- Nitric oxide is required for an optimal establishment of the Medicago truncatula-Sinorhizobium meliloti symbiosisJennifer Del Giudice
UMR INRA 1301 CNRS 6243 Université de Nice Sophia Antipolis, Interactions Biotiques et Santé Végétale, Institut Agrobiotech, 400 Route des Chappes, Sophia Antipolis Cedex, France
New Phytol 191:405-17. 2011..we detected NO production in the first steps, during infection threads growth, of the Medicago truncatula-Sinorhizobium meliloti symbiotic interaction. Nitric oxide was also detected, by confocal microscopy, in nodule primordia...
- Nitric oxide is formed in Medicago truncatula-Sinorhizobium meliloti functional nodulesEmmanuel Baudouin
Interactions Plantes Microorganismes et Santé Végétale, UMR INRA 1064 Université de Nice Sophia Antipolis CNRS 6192, 400, Route des Chappes, BP 167, 06903 Sophia Antipolis, France
Mol Plant Microbe Interact 19:970-5. 2006..Here, we defined when and where NO is produced during Medicago truncatula-Sinorhizobium meliloti symbiosis...
- Evidences of autoregulation of hfq expression in Sinorhizobium meliloti strain 2011Patricio Sobrero
Programa Interacciones Biológicas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina
Arch Microbiol 193:629-39. 2011..In the α-proteobacterium Sinorhizobium meliloti, the large number of detected small RNA transcripts and the pleiotropic effects of hfq mutations lead to ..
- Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110Takakazu Kaneko
Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0812, Japan
DNA Res 9:189-97. 2002..japonicum genes showed significant sequence similarity to those of both Mesorhizobium loti and Sinorhizobium meliloti, while 23% were unique to this species...
- Similarity between the Rhizobium meliloti fliP gene and pathogenicity-associated genes from animal and plant pathogensT M Finan
Department of Biology, McMaster University, Hamilton, Ontario, Canada
Gene 152:65-7. 1995..It is likely that all of these proteins share a common functional role in the secretion of specific proteins from bacterial cells...
- Contribution of NFP LysM domains to the recognition of Nod factors during the Medicago truncatula/Sinorhizobium meliloti symbiosisSandra Bensmihen
Laboratoire des Interactions Plantes Microorganismes, Institut National de la Recherche Agronomique, Castanet Tolosan, France
PLoS ONE 6:e26114. 2011..Nod factor perception (NFP) in the recognition of different substitutions of NFs produced by its symbiont Sinorhizobium meliloti. These substitutions are a sulphate group at the reducing end, which is essential for host specificity, ..
- A survey of sRNA families in α-proteobacteriaCoral Del Val
CITIC UGR, Centro de Investigación en Tecnologías de la Información y de las Comunicaciones de la Universidad de Granada, Granada, Spain
RNA Biol 9:119-29. 2012..meliloti 1021. The distribution patterns of these sRNA families suggest major contributions of vertical inheritance and extensive ancestral duplication events to the evolution of sRNAs in plant-interacting bacteria...
- Pharmacological evidence that multiple phospholipid signaling pathways link Rhizobium nodulation factor perception in Medicago truncatula root hairs to intracellular responses, including Ca2+ spiking and specific ENOD gene expressionDorothée Charron
Laboratoire des Interactions Plantes Microorganismes, Unite Mixte de Recherche, Institut National de la Recherche Agronomique Centre National de la Recherche Scientifique, 31326 Castanet Tolosan, France
Plant Physiol 136:3582-93. 2004....
- GlnB/GlnK PII proteins and regulation of the Sinorhizobium meliloti Rm1021 nitrogen stress response and symbiotic functionSvetlana N Yurgel
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164 6340, USA
J Bacteriol 192:2473-81. 2010The Sinorhizobium meliloti Rm1021 Delta glnD-sm2 mutant, which is predicted to make a GlnD nitrogen sensor protein truncated at its amino terminus, fixes nitrogen in symbiosis with alfalfa, but the plants cannot use this nitrogen for ..
- glnD and mviN are genes of an essential operon in Sinorhizobium melilotiP A Rudnick
Laboratoire de Biologie Moleculaire des relations Plantes Microorganismes, INRA CNRS, 31326 Castanet Tolosan Cedex, France
J Bacteriol 183:2682-5. 2001To evaluate the role of uridylyl-transferase, the Sinorhizobium meliloti glnD gene was isolated by heterologous complementation in Azotobacter vinelandii. The glnD gene is cotranscribed with a gene homologous to Salmonella mviN...
- Resuscitation of viable but not culturable Sinorhizobium meliloti 41 pRP4-luc: effects of oxygen and host plantMarina Basaglia
Dipartimento di Biotecnologie Agrarie, Agripolis Università di Padova, Viale dell Universita 16, 35020 Legnaro, Padova, Italy
Curr Microbiol 54:167-74. 2007A plasmid-borne, firefly-derived, luciferase gene (luc) was inserted and stably inherited in Sinorhizobium meliloti 41 as a reporter gene. The strain obtained, S...
- CbrA is a stationary-phase regulator of cell surface physiology and legume symbiosis in Sinorhizobium melilotiKatherine E Gibson
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Bacteriol 188:4508-21. 2006b>Sinorhizobium meliloti produces an exopolysaccharide called succinoglycan that plays a critical role in promoting symbiosis with its host legume, alfalfa (Medicago sativa)...
- A mutant GlnD nitrogen sensor protein leads to a nitrogen-fixing but ineffective Sinorhizobium meliloti symbiosis with alfalfaSvetlana N Yurgel
Institute of Biological Chemistry and School of Molecular Biosciences, Washington State University, Pullman, WA 99164 6340, USA
Proc Natl Acad Sci U S A 105:18958-63. 2008..In the symbiosis between Sinorhizobium meliloti and alfalfa, mutations in GlnD, the major bacterial nitrogen stress response sensor protein, led to a ..
- L-Canavanine made by Medicago sativa interferes with quorum sensing in Sinorhizobium melilotiNeela D Keshavan
Department of Molecular and Cell Biology, University of Texas at Dallas, FO 3 1, Richardson, TX 75083 0688, USA
J Bacteriol 187:8427-36. 2005b>Sinorhizobium meliloti is a gram-negative soil bacterium, capable of establishing a nitrogen-fixing symbiosis with its legume host, alfalfa (Medicago sativa)...
- Identification of a hydroxyproline transport system in the legume endosymbiont Sinorhizobium melilotiAllyson M MacLean
Center for Environmental Genomics, Department of Biology, McMaster University, Hamilton, Ontario, Canada
Mol Plant Microbe Interact 22:1116-27. 2009..an ABC-type transport system dedicated to the uptake of hydroxyproline in the legume endosymbiont Sinorhizobium meliloti. We have designated genes involved in hydroxyproline metabolism as hyp genes and show that an S...
- Complex regulation of symbiotic functions is coordinated by MucR and quorum sensing in Sinorhizobium melilotiKonrad Mueller
Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX 75080 0688, USA
J Bacteriol 193:485-96. 2011In Sinorhizobium meliloti, the production of exopolysaccharides such as succinoglycan and exopolysaccharide II (EPS II) enables the bacterium to invade root nodules on Medicago sativa and establish a nitrogen-fixing symbiosis...
- pSymA-dependent mobilization of the Sinorhizobium meliloti pSymB megaplasmidHelena Blanca-Ordóñez
Plant Bacteria Interactions Laboratory, Departamento Microbiología del Suelo y Sistemas Simbióticos, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas CSIC, Profesor Albareda 1, Granada, Spain
J Bacteriol 192:6309-12. 2010b>Sinorhizobium meliloti 1021 carries two megaplasmids, pSymA of 1,354 kb and pSymB of 1,683 kb, which are essential in establishing symbiosis with its legume hosts and important for bacterial fitness in the rhizosphere...
- Crystal structures of the choline/acetylcholine substrate-binding protein ChoX from Sinorhizobium meliloti in the liganded and unliganded-closed statesChristine Oswald
Institute of Biochemistry, Heinrich Heine University Duesseldorf, Universitaetsstrasse 1, 40225 Duesseldorf, Germany
J Biol Chem 283:32848-59. 2008The ATP-binding cassette transporter ChoVWX is one of several choline import systems operating in Sinorhizobium meliloti. Here fluorescence-based ligand binding assays were used to quantitate substrate binding by the periplasmic ligand-..
- Formate-dependent autotrophic growth in Sinorhizobium melilotiBrad S Pickering
Department of Microbiology, University if Manitoba, Winnipeg, MB, R3T 2N2, Canada
J Bacteriol 190:6409-18. 2008..Taken together, the data allow us to present a model of how S. meliloti can grow on defined medium containing only formate and bicarbonate as carbon sources...
- LysM domains of Medicago truncatula NFP protein involved in Nod factor perception. Glycosylation state, molecular modeling and docking of chitooligosaccharides and Nod factorsLonneke Mulder
Centre de Recherches sur les Macromolecules Vegetales, CNRS affiliated with Université Joseph Fourier, 601 rue de la Chimie, BP 53, 38041 Grenoble Cedex 9, France
Glycobiology 16:801-9. 2006..In Medicago truncatula, specific recognition of Sinorhizobium meliloti and its Nod factors requires the NFP (Nod factor perception) gene, which encodes a putative serine/..
- Genome variation in the symbiotic nitrogen-fixing bacterium Sinorhizobium melilotiHong Guo
Center for Environmental Genomics, Department of Biology, McMaster University, Hamilton, ON L8S 4K1, Canada
Genome 52:862-75. 2009..the patterns of genome variation among 10 natural strains of the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti, using pulse field gel electrophoresis (PFGE) and whole-genome microarray hybridizations...
- Ectoine-induced proteins in Sinorhizobium meliloti include an Ectoine ABC-type transporter involved in osmoprotection and ectoine catabolismMohamed Jebbar
Universite de Rennes I, UMR CNRS 6026, Departement Osmorégulation chez les Bactéries, Campus de Beaulieu, Av du Général Leclerc, 35042 Rennes, France
J Bacteriol 187:1293-304. 2005To understand the mechanisms of ectoine-induced osmoprotection in Sinorhizobium meliloti, a proteomic examination of S. meliloti cells grown in minimal medium supplemented with ectoine was undertaken...
- Functional analysis of Sinorhizobium meliloti genes involved in biotin synthesis and transportPlamena Entcheva
Institut für Mikrobiologie und Genetik der Universität Göttingen, 37077 Gottingen, Germany
Appl Environ Microbiol 68:2843-8. 2002External biotin greatly stimulates bacterial growth and alfalfa root colonization by Sinorhizobium meliloti strain 1021...
- Identification of novel Sinorhizobium meliloti mutants compromised for oxidative stress protection and symbiosisBryan W Davies
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Bacteriol 189:2110-3. 2007Employing a novel two-part screen, we identified Sinorhizobium meliloti mutants that were both sensitive to hydrogen peroxide and symbiotically defective on the host plant Medicago sativa...
- alpha-Galactoside uptake in Rhizobium meliloti: isolation and characterization of agpA, a gene encoding a periplasmic binding protein required for melibiose and raffinose utilizationD J Gage
Department of Biological Sciences, Howard Hughes Medical Institute, Stanford University, Stanford, California 94305 5020, USA
J Bacteriol 180:5739-48. 1998..meliloti and at low levels in the free-living bacteria, we propose that AgpA may belong to the class of gene products whose expression decreases when R. meliloti becomes an intracellular symbiont...
- Expression of a class 1 hemoglobin gene and production of nitric oxide in response to symbiotic and pathogenic bacteria in Lotus japonicusMaki Nagata
Graduate School of Science and Technology, Kagoshima University, Kagoshima, Japan
Mol Plant Microbe Interact 21:1175-83. 2008..In contrast, inoculation with the nonsymbiotic rhizobia Sinorhizobium meliloti and Bradyrhizobium japonicum induced neither expression of LjHb1 nor NO production. When L...
- Sinorhizobium meliloti mutants lacking phosphotransferase system enzyme HPr or EIIA are altered in diverse processes, including carbon metabolism, cobalt requirements, and succinoglycan productionCatalina Arango Pinedo
Department of Molecular and Cell Biology, University of Connecticut, 91 N Eagleville Rd, U 3125, Storrs, CT 06269 3125, USA
J Bacteriol 190:2947-56. 2008b>Sinorhizobium meliloti is a member of the Alphaproteobacteria that fixes nitrogen when it is in a symbiotic relationship. Genes for an incomplete phosphotransferase system (PTS) have been found in the genome of S. meliloti...
- Differentiation of symbiotic cells and endosymbionts in Medicago truncatula nodulation are coupled to two transcriptome-switchesNicolas Maunoury
Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2355, Gif sur Yvette, France
PLoS ONE 5:e9519. 2010The legume plant Medicago truncatula establishes a symbiosis with the nitrogen-fixing bacterium Sinorhizobium meliloti which takes place in root nodules...
- A Sinorhizobium meliloti lipopolysaccharide mutant altered in cell surface sulfationDavid H Keating
Howard Hughes Medical Institute Department of Biology, Stanford University, Stanford, California 94305, USA
J Bacteriol 184:6681-9. 2002..Nodule development requires a bacterial signal referred to as Nod factor, which in Sinorhizobium meliloti is a beta-(1,4)-linked tetramer of N-acetylglucosamine containing N-acyl and O-acetyl modifications at the ..
- The response to nitric oxide of the nitrogen-fixing symbiont Sinorhizobium melilotiEliane Meilhoc
Laboratoire des Interactions Plantes Microorganismes, UMR441 2594 INRA CNRS BP52627, R 31320 Castanet Tolosan, France
Mol Plant Microbe Interact 23:748-59. 2010..of NO during the symbiotic interaction between the model legume Medicago truncatula and its symbiont Sinorhizobium meliloti have been reported but the role of NO in symbiosis is far from being elucidated...
- Expression and regulation of phosphate stress inducible genes in Sinorhizobium melilotiM L Summers
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman 59717 3120, USA
Mol Plant Microbe Interact 11:1094-101. 1998b>Sinorhizobium meliloti 104A14 was mutated with transposon Tn5B22, which creates lacZ transcriptional fusions when inserted in the correct orientation relative to the promoter...
- Biosynthesis of UDP-xylose and UDP-arabinose in Sinorhizobium meliloti 1021: first characterization of a bacterial UDP-xylose synthase, and UDP-xylose 4-epimeraseXiaogang Gu
Complex Carbohydrate Research Center CCRC, University of Georgia, Athens, GA 30602, USA
Microbiology 157:260-9. 2011b>Sinorhizobium meliloti is a soil bacterium that fixes nitrogen after being established inside nodules that can form on the roots of several legumes, including Medicago truncatula. A mutation in an S...
- Structure and role in symbiosis of the exoB gene of Rhizobium leguminosarum bv trifoliiB Sánchez-Andújar
Departamento de Microbiologia, Facultad de Farmacia, Universidad de Sevilla, Spain
Mol Gen Genet 255:131-40. 1997..The exoB mutant induced the development of abnormal root nodules and was almost completely unable to invade plant cells. Our results stress the importance of exoB in the Rhizobium-plant interaction...
- Expression of Medicago truncatula genes responsive to nitric oxide in pathogenic and symbiotic conditionsAlberto Ferrarini
Dipartimento Scientifico e Tecnologico, Universita degli Studi di Verona, 37134 Verona, Italy
Mol Plant Microbe Interact 21:781-90. 2008..with the foliar pathogen Colletotrichum trifolii race 1 and during the symbiotic interaction with Sinorhizobium meliloti 1,021...
- FixJ: a major regulator of the oxygen limitation response and late symbiotic functions of Sinorhizobium melilotiChristine Bobik
Laboratoire des Interactions Plantes Microorganismes LIPM, UMR441 2594 CNRS INRA BP52627, Chemin de Borde Rouge, Auzeville, 31326 Castanet Tolosan Cedex, France
J Bacteriol 188:4890-902. 2006b>Sinorhizobium meliloti exists either in a free-living state in the soil or in symbiosis within legume nodules, where the bacteria differentiate into nitrogen-fixing bacteroids...
- Interrelations between glycine betaine catabolism and methionine biosynthesis in Sinorhizobium meliloti strain 102F34Lise Barra
Osmorégulation chez les bactéries, UMR CNRS 6026, Universite de Rennes I, Campus de Beaulieu, Av du Général Leclerc, 35042 Rennes, France
J Bacteriol 188:7195-204. 2006Methionine is produced by methylation of homocysteine. Sinorhizobium meliloti 102F34 possesses only one methionine synthase, which catalyzes the transfer of a methyl group from methyl tetrahydrofolate to homocysteine...
- Biochemical characterization of Sinorhizobium meliloti mutants reveals gene products involved in the biosynthesis of the unusual lipid A very long-chain fatty acidAndreas F Haag
School of Medicine and Dentistry, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom
J Biol Chem 286:17455-66. 2011b>Sinorhizobium meliloti forms a symbiosis with the legume alfalfa, whereby it differentiates into a nitrogen-fixing bacteroid. The lipid A species of S...
- Overlap of proteome changes in Medicago truncatula in response to auxin and Sinorhizobium melilotiGiel E van Noorden
Australian Research Council Centre of Excellence for Integrative Legume Research, Australian National University, Canberra, Australian Capital Territory 0200, Australia
Plant Physiol 144:1115-31. 2007..mass spectrometry to compare proteomes of wild-type and sunn mutant roots after 24 h of treatment with Sinorhizobium meliloti, auxin, or a control. We identified 131 of 270 proteins responding to treatment with S...
- Medicago truncatula ENOD11: a novel RPRP-encoding early nodulin gene expressed during mycorrhization in arbuscule-containing cellsE P Journet
Laboratoire de Biologie Moleculaire des relations Plantes Microorganismes, CNRS INRA, Castanet Tolosan, France
Mol Plant Microbe Interact 14:737-48. 2001..The potential role of early nodulins with atypical RPRP structures such as ENOD11 and ENOD12 in symbiotic and nonsymbiotic cellular contexts is discussed...
- The MtMMPL1 early nodulin is a novel member of the matrix metalloendoproteinase family with a role in Medicago truncatula infection by Sinorhizobium melilotiJean Philippe Combier
Laboratoire des Interactions Plantes Micro organismes, Centre National de la Recherche Scientifique Institut National de la Recherche Agronomique, 31326 Castanet Tolosan Cedex, France
Plant Physiol 144:703-16. 2007..identified by transcriptomics, represents a novel and specific marker for root and nodule infection by Sinorhizobium meliloti. This was established by determining the spatial pattern of MtMMPL1 expression and evaluating gene ..
- Transcriptome analysis of Sinorhizobium meliloti during symbiosisFrederic Ampe
Laboratoire de Biologie Moleculaire des relations Plantes Microorganismes, UMR 215 Centre National de la Recherche Scientifique Institut National de la Recherche Agronomique, BP27 31326 Castanet Tolosan Cedex, France
Genome Biol 4:R15. 2003..global view of the genetics underlying this plant-microbe symbiosis, we have mined the recently determined Sinorhizobium meliloti genome sequence for genes potentially relevant to symbiosis...
- A highly conserved Sinorhizobium meliloti operon is induced microaerobically via the FixLJ system and by nitric oxide (NO) via NnrRFrans J de Bruijn
Laboratoire des Interactions Plantes Micro organismes LIPM, UMR CNRS 2594 INRA 441, BP 52627, 31326 Castanet Tolosan Cedex, France
Environ Microbiol 8:1371-81. 2006A previously generated collection of 11 Tn5-luxAB insertion mutants of Sinorhizobium meliloti harbouring lux reporter gene fusions induced under microaerobic (1% O2) conditions was further characterized and mapped on the sequenced S...
- Sinorhizobium meliloti rpoE2 is necessary for H(2)O(2) stress resistance during the stationary growth phaseMaud Flechard
CNRS UMR6026 DUALS, Universite Rennes I, Rennes, France
FEMS Microbiol Lett 290:25-31. 2009RpoE2 is an extracytoplasmic sigma factor produced by Sinorhizobium meliloti during stationary growth phase. Its inactivation affected the synthesis of the superoxide dismutase, SodC, and catalase, KatC...
- Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formationLuciana Rinaudi
Departamento de Biologia Molecular, Universidad Nacional de Rio Cuarto, 5800 Rio Cuarto, Cordoba, Argentina
Res Microbiol 157:867-75. 2006..assays to study environmental parameters that might influence biofilm formation in the Medicago symbiont Sinorhizobium meliloti. These parameters include carbon source, amount of nitrate, phosphate, calcium and magnesium as well as ..
- Identification and characterization of nodulation-signaling pathway 2, a gene of Medicago truncatula involved in Nod actor signalingGiles E D Oldroyd
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Plant Physiol 131:1027-32. 2003..nsp2 plants also show a complete absence of infection and cortical cell division following Sinorhizobium meliloti inoculation...
- Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58B Goodner
Department of Biology, Hiram College, Hiram, OH 44234, USA
Science 294:2323-8. 2001..We discuss genome architecture and evolution and additional genes potentially involved in virulence and metabolic parasitism of host plants...
- Stable low molecular weight RNA profiling showed variations within Sinorhizobium meliloti and Sinorhizobium medicae nodulating different legumes from the alfalfa cross-inoculation groupMaría Del Villar
Departamento de Microbiologia y Genetica, Universidad de Salamanca, Spain
FEMS Microbiol Lett 282:273-81. 2008..of each LMW RNA profile it was evident that isolates with LMW RNA profiles I and II belonged to Sinorhizobium meliloti and those displaying profiles III and IV to Sinorhizobium medicae...
- Mutational analysis of the Sinorhizobium meliloti short-chain dehydrogenase/reductase family reveals substantial contribution to symbiosis and catabolic diversityAsha I Jacob
Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L 3G1, Canada
Mol Plant Microbe Interact 21:979-87. 2008..study, we carried out a large-scale mutagenesis of the SDR gene family in the alfalfa root nodule symbiont Sinorhizobium meliloti. Subsequent phenotypic analysis revealed phenotypes for mutants of 21 of the SDR-encoding genes...
- CYCLE REGULATION IN THE SYMBIONT SINORHIZOBIUM MELILOTIKATHERINE ELISABETH GIBSON; Fiscal Year: 2011..cell cycle occurs during chronic host colonization in a variety of species, including our model organism Sinorhizobium meliloti. Insights gained from our analysis of cell cycle regulation in S...
- Regulatory Circuits Controlling Development of Dormant Microbial CystsCARL EUGENE BAUER; Fiscal Year: 2013..such as Azospirillum brasilense, Rhizobium leguminosarum, Mesorhizobium loti, Sinorhizobium medicae, Sinorhizobium meliloti, Sinorhizobium fredii, Delftia acidovorans (Comamonas acidovorans), Burkholderia mallei (Pseudomonas ..
- Structural and functional studies of autoinducer-2 processing proteinsStephen T Miller; Fiscal Year: 2012..coli LsrF and LsrG and a putative functional homolog of LsrF from Sinorhizobium meliloti. Cell extracts will be analyzed by mass spectrometry and NMR to identify the products of these reactions...
- Control of Symbiotic Gene Expression in Sinorhizobium melilotiSharon R Long; Fiscal Year: 2013..These signal exchanges are critical to pathogenesis and to symbiosis. We study Sinorhizobium meliloti, an alpha- proteobacterium, which establishes a beneficial nitrogen fixing symbiosis with host plants ..
- Regulatory network of a conserved polar factor in Sinorhizobium melilotiJOSEPH CHIUNG CHU CHEN; Fiscal Year: 2013..conserved polar factor controls multiple cell envelope-associated activities in the alpha- proteobacterium Sinorhizobium meliloti, which infects the roots of legume plants to form symbiosis...
- Regulated Assembly of Subcellular Structures in alpha-ProteobacteriaJOSEPH CHIUNG CHU CHEN; Fiscal Year: 2010..The regulatory components are conserved in Sinorhizobium meliloti, a related bacterium that induces nodule formation in plant roots during symbiosis. However, S...
- A periplasmic global regulator, ExoR, for Bacterial invasion of host cellsHai Ping Cheng; Fiscal Year: 2012..bacterial sensing and signaling during host cell invasion, we are studying the molecular mechanism of Sinorhizobium meliloti ExoR protein in controlling the switch from free living cells to invasion ready cells...
- Quorum Sensing in Sinorhizobium melilotiJuan Gonzalez; Fiscal Year: 2009The symbiotic relationship between the nitrogen-fixing Sinorhizobium meliloti and its legume host (Medicago sativa) is the result of an intricate signaling network between the host and the symbiont...
- Biosynthesis and Physiology of Vitamin B12 in ProkaryotesMICHIKO TAGA; Fiscal Year: 2009..identified BluB, an enzyme that catalyzes the production of DMB in the symbiotic nitrogen fixing bacterium Sinorhizobium meliloti. The bluB mutant of S...
- STRUCTURE AND DYNAMICS OF HEME PROTEIN ACTIVE SITESJAMES SATTERLEE; Fiscal Year: 2005..These include the FixLs from Bradyrhizobium japonicum (BjFixL) and Sinorhizobium meliloti (SmFixL) and the Direct Oxygen Sensor protein from E. coli (EcDos)...
- Modulation of two-component signaling in alphaproteobacteriaJoel Griffitts; Fiscal Year: 2009..Using the plant symbiont Sinorhizobium meliloti as a model for chronic infection, a novel `two-component'phosphotransfer system required for cyclic ..
- Heme as an iron source in Sinorhizobium melilotiMARK O BRIAN; Fiscal Year: 2008..It was shown recently that S. meliloti uses heme as a nutritional iron source in Sinorhizobium meliloti and related species. The ?-proteobacterial group to which S...
- Evolution of Estrogen Receptor-Ligand InteractionsJennifer Fox; Fiscal Year: 2006..Such an experimental system will allow the rigorous testing of hypotheses about ER-ligand structure function relationships and the dynamics of receptor-ligand coevolution. ..
- Siderophore transport mechanism via membrane receptorsRanjan Chakraborty; Fiscal Year: 2004..For the mutant proteins showing normal binding and defective transport, methods of purification and crystallization will be standardized. ..
- Genetic Systems Bioengineering for Escherichia coliAlexander Ninfa; Fiscal Year: 2009..In addition we propose designs for novel genetic clocks that test the role of various system design principles in the production of biological oscillations. ..
- Elucidating the Conformational Dynamics of the AAA+ ATPases NtrC1 and NtrCB Nixon; Fiscal Year: 2009..In the course of performing this research, new tools will be developed that are expected to be broadly applicable to similar studies of other proteins that are vital for human health. ..
- Structure/function analysis of the E. coli NIR/NRII signal transduction systemAlexander Ninfa; Fiscal Year: 2009..Our studies with a model system should allow rapid progress. ..
- Phenanthroline Derivatives in Asymmetric CatalysisElke Schoffers; Fiscal Year: 2003..These experimental approaches will be complemented with computational ligand design based on molecular mechanics tools. ..
- INTERACTIONS OF THE SIGNAL TRANSDUCTION PROTEIN GLNKAlexander Ninfa; Fiscal Year: 2002..These experiments should elucidate the design of the signal transduction system controlling nitrogen assimilation, and the mechanisms of interaction of PII and GlnK with their receptors and small molecule effectors. ..
- MOLECULAR SEQUENCE DATASamuel Karlin; Fiscal Year: 2001..by spacings between repeats, and by properties of repeat families (intergenic, coding, direct, inverted, mixed). ..
- Gene Expression Levels Across Diverse GenomesSamuel Karlin; Fiscal Year: 2006..A third major objective of our research will be to extend our codon usage methods for predicting gene expression levels to eukaryotic genomes, including yeast, D. melanogaster, C. elegans, and human. [unreadable] [unreadable]..