lactococcus lactis

Summary

Summary: A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.

Top Publications

  1. ncbi Time-resolved genetic responses of Lactococcus lactis to a dairy environment
    Herwig Bachmann
    NIZO Food Research, Ede, The Netherlands
    Environ Microbiol 12:1260-70. 2010
  2. ncbi Phage abortive infection in lactococci: variations on a theme
    Marie Christine Chopin
    Unité de Génétique Microbienne, INRA, CRJJ, 78352 Jouy en Josas Cedex, France
    Curr Opin Microbiol 8:473-9. 2005
  3. ncbi A general system for generating unlabelled gene replacements in bacterial chromosomes
    K Leenhouts
    Department of Genetics, University of Groningen, Haren, The Netherlands
    Mol Gen Genet 253:217-24. 1996
  4. pmc Identification of the receptor-binding protein in 936-species lactococcal bacteriophages
    Kitt Dupont
    Department of Food Science, The Royal Veterinary and Agricultural University, DK 1958 Frederiksberg C, Denmark
    Appl Environ Microbiol 70:5818-24. 2004
  5. ncbi Characterization of AcmB, an N-acetylglucosaminidase autolysin from Lactococcus lactis
    Carine Huard
    Unité de Biochimie et Structure des Protéines, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Microbiology 149:695-705. 2003
  6. pmc Investigation of the adaptation of Lactococcus lactis to isoleucine starvation integrating dynamic transcriptome and proteome information
    Clémentine Dressaire
    Universite de Toulouse INSA, UPS, INP LISBP, 135 Avenue de Rangueil, F 31077 Toulouse, France
    Microb Cell Fact 10:S18. 2011
  7. pmc Peptidoglycan structure analysis of Lactococcus lactis reveals the presence of an L,D-carboxypeptidase involved in peptidoglycan maturation
    Pascal Courtin
    INRA, Unité de Biochimie Bactérienne, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    J Bacteriol 188:5293-8. 2006
  8. ncbi Overview on sugar metabolism and its control in Lactococcus lactis - the input from in vivo NMR
    Ana Rute Neves
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Rua da Quinta Grande, 6, Apt 127, 2780 156 Oeiras, Portugal
    FEMS Microbiol Rev 29:531-54. 2005
  9. pmc Genome-scale diversity and niche adaptation analysis of Lactococcus lactis by comparative genome hybridization using multi-strain arrays
    Roland J Siezen
    Kluyver Centre for Genomics of Industrial Fermentation, NIZO Food Research, P O Box 20, 6710 BA Ede, The Netherlands
    Microb Biotechnol 4:383-402. 2011
  10. ncbi Sequence and comparative genomic analysis of lactococcal bacteriophages jj50, 712 and P008: evolutionary insights into the 936 phage species
    Jennifer Mahony
    Department of Microbiology, University College Cork, Ireland
    FEMS Microbiol Lett 261:253-61. 2006

Research Grants

Detail Information

Publications294 found, 100 shown here

  1. ncbi Time-resolved genetic responses of Lactococcus lactis to a dairy environment
    Herwig Bachmann
    NIZO Food Research, Ede, The Netherlands
    Environ Microbiol 12:1260-70. 2010
    b>Lactococcus lactis is one of main bacterial species found in mixed dairy starter cultures for the production of semi-hard cheese...
  2. ncbi Phage abortive infection in lactococci: variations on a theme
    Marie Christine Chopin
    Unité de Génétique Microbienne, INRA, CRJJ, 78352 Jouy en Josas Cedex, France
    Curr Opin Microbiol 8:473-9. 2005
    ..Twenty Abi systems have been isolated in Lactococcus lactis, a bacterium used in cheese-making fermentation processes, where phage attacks are of economical importance...
  3. ncbi A general system for generating unlabelled gene replacements in bacterial chromosomes
    K Leenhouts
    Department of Genetics, University of Groningen, Haren, The Netherlands
    Mol Gen Genet 253:217-24. 1996
    ..A feasibility study was performed using Lactococcus lactis and Bacillus subtilis as model organisms...
  4. pmc Identification of the receptor-binding protein in 936-species lactococcal bacteriophages
    Kitt Dupont
    Department of Food Science, The Royal Veterinary and Agricultural University, DK 1958 Frederiksberg C, Denmark
    Appl Environ Microbiol 70:5818-24. 2004
    ..The recombinant phages were able to form plaques on the sk1 host Lactococcus lactis MG1614, and recombination was verified by PCR analysis directly with the plaques...
  5. ncbi Characterization of AcmB, an N-acetylglucosaminidase autolysin from Lactococcus lactis
    Carine Huard
    Unité de Biochimie et Structure des Protéines, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Microbiology 149:695-705. 2003
    ..hydrolase, named acmB, which is a paralogue of the major autolysin acmA gene, was identified in the Lactococcus lactis genome sequence. The acmB gene is transcribed in L...
  6. pmc Investigation of the adaptation of Lactococcus lactis to isoleucine starvation integrating dynamic transcriptome and proteome information
    Clémentine Dressaire
    Universite de Toulouse INSA, UPS, INP LISBP, 135 Avenue de Rangueil, F 31077 Toulouse, France
    Microb Cell Fact 10:S18. 2011
    ..The global response of the LAB model Lactococcus lactis ssp...
  7. pmc Peptidoglycan structure analysis of Lactococcus lactis reveals the presence of an L,D-carboxypeptidase involved in peptidoglycan maturation
    Pascal Courtin
    INRA, Unité de Biochimie Bactérienne, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    J Bacteriol 188:5293-8. 2006
    Detailed structural analysis of Lactococcus lactis peptidoglycan was achieved by identification of its constituent muropeptides separated by reverse phase high-performance liquid chromatography...
  8. ncbi Overview on sugar metabolism and its control in Lactococcus lactis - the input from in vivo NMR
    Ana Rute Neves
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Rua da Quinta Grande, 6, Apt 127, 2780 156 Oeiras, Portugal
    FEMS Microbiol Rev 29:531-54. 2005
    ..The relative metabolic simplicity and the availability of genetic tools made Lactococcus lactis the organism of choice to gain insight into metabolic and regulatory networks...
  9. pmc Genome-scale diversity and niche adaptation analysis of Lactococcus lactis by comparative genome hybridization using multi-strain arrays
    Roland J Siezen
    Kluyver Centre for Genomics of Industrial Fermentation, NIZO Food Research, P O Box 20, 6710 BA Ede, The Netherlands
    Microb Biotechnol 4:383-402. 2011
    b>Lactococcus lactis produces lactic acid and is widely used in the manufacturing of various fermented dairy products. However, the species is also frequently isolated from non-dairy niches, such as fermented plant material...
  10. ncbi Sequence and comparative genomic analysis of lactococcal bacteriophages jj50, 712 and P008: evolutionary insights into the 936 phage species
    Jennifer Mahony
    Department of Microbiology, University College Cork, Ireland
    FEMS Microbiol Lett 261:253-61. 2006
    ..These genetic differences may be responsible for the observed differential sensitivity to the lactococcal DNA injection blocking protein, Sie(2009), and the abortive infection system, AbiA...
  11. pmc Neonatal mucosal immunization with a non-living, non-genetically modified Lactococcus lactis vaccine carrier induces systemic and local Th1-type immunity and protects against lethal bacterial infection
    K Ramirez
    Department of Pediatrics, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Mucosal Immunol 3:159-71. 2010
    ..the capacity of a novel mucosal antigen delivery system consisting of non-living, non-genetically modified Lactococcus lactis particles, designated as Gram-positive enhancer matrix (GEM), to induce immune responses in the neonatal ..
  12. pmc Effect of different NADH oxidase levels on glucose metabolism by Lactococcus lactis: kinetics of intracellular metabolite pools determined by in vivo nuclear magnetic resonance
    Ana Rute Neves
    Instituto de Tecnologia Química e Biológica Universidade Nova de Lisboa and Instituto de Biologia Experimental e Tecnológica, 2780 156 Oeiras, Portugal
    Appl Environ Microbiol 68:6332-42. 2002
    Three isogenic strains of Lactococcus lactis with different levels of H(2)O-forming NADH oxidase activity were used to study the effect of oxygen on glucose metabolism: the parent strain L...
  13. ncbi Experimental conditions affect the site of tetrazolium violet reduction in the electron transport chain of Lactococcus lactis
    Sybille Tachon
    INRA, UR 477 Biochimie Bactérienne, F 78350 Jouy en Josas, France
    Microbiology 155:2941-8. 2009
    ..The objective of the present study was to identify the reduction mechanisms of tetrazolium violet (TV) in Lactococcus lactis using a mutagenesis approach, under two experimental conditions generally applied in microbiology: a plate ..
  14. ncbi A Plasmodium falciparum GLURP-MSP3 chimeric protein; expression in Lactococcus lactis, immunogenicity and induction of biologically active antibodies
    Michael Theisen
    Department of Clinical Biochemistry, Statens Serum Institut, Copenhagen, Denmark
    Vaccine 22:1188-98. 2004
    ..falciparum Merozoite surface protein 3 (MSP3) was produced in Lactococcus lactis as a secreted recombinant GLURP-MSP3 fusion protein...
  15. pmc Novel surface display system for proteins on non-genetically modified gram-positive bacteria
    Tjibbe Bosma
    BiOMaDe Technology, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Appl Environ Microbiol 72:880-9. 2006
    ..This binding domain, the protein anchor (PA), was derived from the Lactococcus lactis peptidoglycan hydrolase AcmA. GEM particles were typically prepared from the innocuous bacterium L...
  16. ncbi Prevention and treatment of colitis with Lactococcus lactis secreting the immunomodulatory Yersinia LcrV protein
    Benoit Foligne
    Laboratoire des Bactéries Lactiques et Immunité des Muqueuses, Institut Pasteur de Lille, Lille, France
    Gastroenterology 133:862-74. 2007
    ....
  17. ncbi Linear covariance models to examine the determinants of protein levels in Lactococcus lactis
    Clémentine Dressaire
    Universite de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F 31077 Toulouse, France
    Mol Biosyst 6:1255-64. 2010
    ..by statistical modelling, of the major determinants of protein concentrations in a bacterial model Lactococcus lactis. Protein concentrations were predicted by covariance models taking into account various quantitative and ..
  18. ncbi Conservation of key elements of natural competence in Lactococcus lactis ssp
    Sandra Wydau
    Génétique Microbienne, INRA, Domaine de Vilvert, Jouy en Josas Cedex, France
    FEMS Microbiol Lett 257:32-42. 2006
    ..Recently, the genome sequence of various species, including Lactococcus lactis, revealed the presence of homologues of competence genes in bacteria, which were not previously identified ..
  19. ncbi The anaerobic (class III) ribonucleotide reductase from Lactococcus lactis. Catalytic properties and allosteric regulation of the pure enzyme system
    E Torrents
    Department of Biochemistry 1, Medical Nobel Institute, MBB, Karolinska Institutet, S 17177 Stockholm, Sweden
    J Biol Chem 275:2463-71. 2000
    b>Lactococcus lactis contains an operon with the genes (nrdD and nrdG) for a class III ribonucleotide reductase. Strict anaerobic growth depends on the activity of these genes...
  20. ncbi HtrA is a key factor in the response to specific stress conditions in Lactococcus lactis
    Catherine Foucaud-Scheunemann
    INRA, Unité de Recherches Laitières et Génétique Appliquée, 78352 Jouy en Josas, France
    FEMS Microbiol Lett 224:53-9. 2003
    We investigated the physiological role of Lactococcus lactis housekeeping surface protease HtrA. It is involved in surface properties under regular growth conditions, as the htrA mutant strain forms longer chains in liquid medium...
  21. pmc Acetate utilization in Lactococcus lactis deficient in lactate dehydrogenase: a rescue pathway for maintaining redox balance
    P Hols
    Microbial Ingredients Section, NIZO Food Research and Wageningen Centre for Food Science, 6710 BA Ede, The Netherlands
    J Bacteriol 181:5521-6. 1999
    Acetate was shown to improve glucose fermentation in Lactococcus lactis deficient in lactate dehydrogenase...
  22. pmc Complete sequences of four plasmids of Lactococcus lactis subsp. cremoris SK11 reveal extensive adaptation to the dairy environment
    Roland J Siezen
    Centre for Molecular andBiomolecular Informatics, Radboud University, P O Box 9010, 6500GL Nijmegen, The Netherlands
    Appl Environ Microbiol 71:8371-82. 2005
    b>Lactococcus lactis strains are known to carry plasmids encoding industrially important traits. L. lactis subsp. cremoris SK11 is widely used by the dairy industry in cheese making...
  23. ncbi Dynamic response of catabolic pathways to autoacidification in Lactococcus lactis: transcript profiling and stability in relation to metabolic and energetic constraints
    Sergine Even
    Laboratorie Biotechnologie Bioprocédés, UMR 5504 INSA CNRS and UMR 792 INSA INRA, Centre de Bioingenierie Gilbert Durand, Institute National des Sciences Appliquées, Toulouse, France
    Mol Microbiol 45:1143-52. 2002
    ..the central metabolic pathways to autoacidification (accumulation of organic acid fermentation products) in Lactococcus lactis was investigated in a global manner by integrating molecular data (cellular transcript concentrations, mRNA ..
  24. ncbi Generation and evaluation of A2-expressing Lactococcus lactis live vaccines against Leishmania donovani in BALB/c mice
    Karen K Yam
    Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada
    J Med Microbiol 60:1248-60. 2011
    ..efficacy of Leishmania vaccines engineered from the non-colonizing, non-pathogenic Gram-positive bacterium Lactococcus lactis. A truncated, codon-optimized version of the A2 antigen from Leishmania donovani was engineered for ..
  25. ncbi Prophylaxis of experimentally induced ovomucoid allergy in neonatal pigs using Lactococcus lactis
    P Rupa
    Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada
    Vet Immunol Immunopathol 140:23-9. 2011
    Probiotic Lactococcus lactis (LL) is immunomodulatory and may prevent allergy by biasing from type-2 to a type-1 immune response...
  26. pmc Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403
    Brice Sperandio
    Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
    J Bacteriol 187:3762-78. 2005
    ..for the synthesis of cysteine and methionine and their interconversion were experimentally determined for Lactococcus lactis, a lactic acid bacterium commonly found in food...
  27. pmc LmrR is a transcriptional repressor of expression of the multidrug ABC transporter LmrCD in Lactococcus lactis
    Herfita Agustiandari
    Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands
    J Bacteriol 190:759-63. 2008
    LmrCD is an ABC-type multidrug transporter in Lactococcus lactis. LmrR encodes a putative transcriptional regulator. In a DeltalmrR strain, lmrCD is up-regulated...
  28. pmc Effect of exopolysaccharides on phage-host interactions in Lactococcus lactis
    Hélène Deveau
    Departement de Biochimie et de Microbiologie, Faculte des sciences et de genie, Groupe de Recherche en Ecologie Buccale GREB, Faculte de Medecine Dentaire, Universite Laval, Quebec, Canada G1K 7P4
    Appl Environ Microbiol 68:4364-9. 2002
    In this study, we report that Lactococcus lactis strains producing exopolysaccharides (EPS) are sensitive to virulent phages...
  29. pmc Complete genome sequence of Lactococcus lactis subsp. lactis CV56, a probiotic strain isolated from the vaginas of healthy women
    Yong Gao
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No 1 Beichen West Road, Chaoyang District, Beijing 100101, People s Republic of China
    J Bacteriol 193:2886-7. 2011
    ..Here, we report the finished and annotated genome of a Lactococcus strain that was isolated from the vaginas of healthy women and shows probiotic properties, including nisin A production and adhesion to vaginal epithelial cells...
  30. pmc Microbial domestication signatures of Lactococcus lactis can be reproduced by experimental evolution
    Herwig Bachmann
    NIZO Food Research, 6710BA Ede, The Netherlands
    Genome Res 22:115-24. 2012
    ..The lactic acid bacterium Lactococcus lactis naturally occurs on plants and in dairy environments, and it is proposed that dairy strains originate from ..
  31. pmc Population genomics and phylogeography of an Australian dairy factory derived lytic bacteriophage
    Eduardo Castro-Nallar
    Department of Biology, Brigham Young University
    Genome Biol Evol 4:382-93. 2012
    ..we present the full genomic sequences and evolutionary analyses of a serially sampled population of 28 Lactococcus lactis-infecting phage belonging to the 936-like group in Australia...
  32. pmc A transcriptional activator, homologous to the Bacillus subtilis PurR repressor, is required for expression of purine biosynthetic genes in Lactococcus lactis
    M Kilstrup
    Department of Microbiology, Technical University of Denmark, DK2800 Lyngby, Denmark
    J Bacteriol 180:3907-16. 1998
    A purR::pGh9:ISS1 mutant of Lactococcus lactis was obtained following transposon mutagenesis of strain MG1363 and selection for purine auxotrophs...
  33. ncbi Overall control of nitrogen metabolism in Lactococcus lactis by CodY, and possible models for CodY regulation in Firmicutes
    Eric Guedon
    Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
    Microbiology 151:3895-909. 2005
    ..species of Gram-positive bacteria, was previously described to be the central regulator of proteolysis in Lactococcus lactis. In this study, over 100 potential CodY targets were identified by DNA-microarray analysis...
  34. pmc Genome sequences of Lactococcus lactis MG1363 (revised) and NZ9000 and comparative physiological studies
    Daniel M Linares
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands
    J Bacteriol 192:5806-12. 2010
    b>Lactococcus lactis NZ9000 and its parent MG1363 are the most commonly used lactic acid bacteria for expression and physiological studies. We noted unexpected but significant differences in the growth behaviors of both strains...
  35. ncbi Oral administration of an IL-10-secreting Lactococcus lactis strain prevents food-induced IgE sensitization
    Christophe P Frossard
    University Hospital of Geneva, Department of Pediatrics, Geneva, Switzerland
    J Allergy Clin Immunol 119:952-9. 2007
    ..Because tolerance to food is potentially modulated by IL-10, strategies to prevent food allergy should favor an increased delivery of IL-10 to the gut...
  36. pmc Induction of heat shock proteins DnaK, GroEL, and GroES by salt stress in Lactococcus lactis
    M Kilstrup
    Department of Microbiology, Technical University of Denmark, Lyngby, Denmark
    Appl Environ Microbiol 63:1826-37. 1997
    The bacterium Lactococcus lactis has become a model organism in studies of growth physiology and membrane transport, as a result of its simple fermentative metabolism...
  37. ncbi A topological model of the baseplate of lactococcal phage Tuc2009
    Giuliano Sciara
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS, 163 Avenue de Luminy Case 932, Marseille Cedex 09, France
    J Biol Chem 283:2716-23. 2008
    Phages infecting Lactococcus lactis, a Gram-positive bacterium, are a recurrent problem in the dairy industry...
  38. pmc Intranasal delivery of influenza subunit vaccine formulated with GEM particles as an adjuvant
    Vinay Saluja
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, 9713 AV, Groningen, The Netherlands
    AAPS J 12:109-16. 2010
    ..use of a safe adjuvant Gram-positive enhancer matrix (GEM) particles derived from the food-grade bacterium Lactococcus lactis for i.n. vaccination with subunit influenza vaccine in mice...
  39. ncbi Examination of post-transcriptional regulations in prokaryotes by integrative biology
    Flora Picard
    Universite de Toulouse, INSA, UPS, INP, LISBP, 135, Avenue de Rangueil, 31077 Toulouse, France
    C R Biol 332:958-73. 2009
    ..In this review, we report mechanisms involved in mRNA and protein stability and translation regulation in prokaryotes, and their dependence on growth phase and environmental growth conditions is particularly highlighted...
  40. pmc Complete genome sequence of the prototype lactic acid bacterium Lactococcus lactis subsp. cremoris MG1363
    Udo Wegmann
    Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    J Bacteriol 189:3256-70. 2007
    b>Lactococcus lactis is of great importance for the nutrition of hundreds of millions of people worldwide. This paper describes the genome sequence of Lactococcus lactis subsp...
  41. pmc Receptor-binding protein of Lactococcus lactis phages: identification and characterization of the saccharide receptor-binding site
    Denise M Tremblay
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS and Universités d Aix Marseille I and II, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France
    J Bacteriol 188:2400-10. 2006
    Phage p2, a member of the lactococcal 936 phage species, infects Lactococcus lactis strains by binding initially to specific carbohydrate receptors using its receptor-binding protein (RBP)...
  42. ncbi Use of Lactococcus lactis expressing pili from group B Streptococcus as a broad-coverage vaccine against streptococcal disease
    Scilla Buccato
    Novartis Vaccines, 1 53100 Siena, Italy
    J Infect Dis 194:331-40. 2006
    ..Here, we show that GBS pilin island 1, transferred into the nonpathogenic microorganism Lactococcus lactis, leads to pilus assembly...
  43. ncbi Mucosal co-application of lactic acid bacteria and allergen induces counter-regulatory immune responses in a murine model of birch pollen allergy
    Andreas Repa
    Department of Pathophysiology, Medical School AKH EBO3Q, University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Vaccine 22:87-95. 2003
    ..In this study, we aimed at evaluating the immunomodulatory potential of two LAB strains, Lactococcus lactis and Lactobacillus plantarum, for prophylaxis and therapy of allergic immune responses...
  44. pmc Structure of lactococcal phage p2 baseplate and its mechanism of activation
    Giuliano Sciara
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 Centre National de la Recherche Scientifique and Universités d Aix Marseille I and II, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France
    Proc Natl Acad Sci U S A 107:6852-7. 2010
    ..All known siphophages infecting gram+ Lactococcus lactis possess a baseplate at the tip of their tail involved in host recognition and attachment...
  45. ncbi Molecular characterization of the plasmid-encoded eps gene cluster essential for exopolysaccharide biosynthesis in Lactococcus lactis
    R van Kranenburg
    Department of Biophysical Chemistry, NIZO, Ede, The Netherlands
    Mol Microbiol 24:387-97. 1997
    b>Lactococcus lactis strain NIZO B40 produces an extracellular phosphopolysaccharide containing galactose, glucose, and rhamnose...
  46. ncbi 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis
    Igor Mierau
    NIZO Food Research, P O Box 20, 6710, Ede, The Netherlands
    Appl Microbiol Biotechnol 68:705-17. 2005
    b>Lactococcus lactis is a Gram-positive lactic acid bacterium that, in addition to its traditional use in food fermentations, is increasingly used in modern biotechnological applications...
  47. pmc Genome2D: a visualization tool for the rapid analysis of bacterial transcriptome data
    Richard J S Baerends
    Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    Genome Biol 5:R37. 2004
    ..The compiled version of Genome2D is freely available for academic or non-profit use from http://molgen.biol.rug.nl/molgen/research/molgensoftware.php...
  48. pmc Evolution of Lactococcus lactis phages within a cheese factory
    Geneviève M Rousseau
    Departement de Biochimie et de Microbiologie, Groupe de Recherche en Ecologie Buccale, Felix d Herelle Reference Center for Bacterial Viruses, Universite Laval, Quebec G1V 0A6, Canada
    Appl Environ Microbiol 75:5336-44. 2009
    ..These six phages infected the same two industrial Lactococcus lactis strains out of 30 tested...
  49. pmc Lactococcus lactis expressing either Staphylococcus aureus fibronectin-binding protein A or Listeria monocytogenes internalin A can efficiently internalize and deliver DNA in human epithelial cells
    Silvia Innocentin
    Unite de Virologie et Immunologie Moleculaires, UR892 INRA, Domaine de Vilvert, F78352 Jouy en Josas Cedex, France
    Appl Environ Microbiol 75:4870-8. 2009
    ..enhanced green fluorescent protein (eGFP) expression plasmid vector was delivered in epithelial cells by Lactococcus lactis producing Listeria monocytogenes internalin A (L...
  50. ncbi High-throughput cloning and expression in recalcitrant bacteria
    Eric R Geertsma
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Nat Methods 4:705-7. 2007
    ..We demonstrated VBEx proof of principle for Lactococcus lactis, but the method can be adapted to all organisms for which plasmids are available.
  51. ncbi Peptidoglycan N-acetylglucosamine deacetylation decreases autolysis in Lactococcus lactis
    Mickael Meyrand
    INRA, Unité de Biochimie Bactérienne, UR477, 78350 Jouy en Josas, France
    Microbiology 153:3275-85. 2007
    The gene xynD (renamed pgdA) of Lactococcus lactis IL1403 was shown to encode a peptidoglycan N-acetylglucosamine deacetylase. Inactivation of pgdA in L...
  52. pmc Genome-scale genotype-phenotype matching of two Lactococcus lactis isolates from plants identifies mechanisms of adaptation to the plant niche
    Roland J Siezen
    NIZO Food Research, P O Box 20, 6710 BA Ede, The Netherlands
    Appl Environ Microbiol 74:424-36. 2008
    b>Lactococcus lactis is a primary constituent of many starter cultures used for the manufacturing of fermented dairy products, but the species also occurs in various nondairy niches such as (fermented) plant material...
  53. ncbi Is the glycolytic flux in Lactococcus lactis primarily controlled by the redox charge? Kinetics of NAD(+) and NADH pools determined in vivo by 13C NMR
    Ana Rute Neves
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    J Biol Chem 277:28088-98. 2002
    The involvement of nicotinamide adenine nucleotides (NAD(+), NADH) in the regulation of glycolysis in Lactococcus lactis was investigated by using (13)C and (31)P NMR to monitor in vivo the kinetics of the pools of NAD(+), NADH, ATP, ..
  54. pmc Genetic manipulation of Lactococcus lactis by using targeted group II introns: generation of stable insertions without selection
    Courtney L Frazier
    Department of Viticulture and Enology, University of California at Davis, Davis, California 95616 8749, USA
    Appl Environ Microbiol 69:1121-8. 2003
    ..efficiently into genes encoding malate decarboxylase (mleS) and tetracycline resistance (tetM) within the Lactococcus lactis genome. Integrants were readily identified and maintained in the absence of a selectable marker...
  55. ncbi Treatment of murine colitis by Lactococcus lactis secreting interleukin-10
    L Steidler
    Department of Molecular Biology, Ghent University and Flanders Interuniversity Institute for Biotechnology, K L Ledeganckstraat 35, 9000 Gent, Belgium
    Science 289:1352-5. 2000
    ..Intragastric administration of IL-10-secreting Lactococcus lactis caused a 50% reduction in colitis in mice treated with dextran sulfate sodium and prevented the onset of ..
  56. ncbi Mucosal vaccine delivery of antigens tightly bound to an adjuvant particle made from food-grade bacteria
    Maarten L van Roosmalen
    BiOMaDe Technology Foundation, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Methods 38:144-9. 2006
    ..This binding domain, the protein anchor (PA), is derived from the Lactococcus lactis AcmA cell-wall hydrolase, and contains three repeats of a LysM-type cell-wall binding motif...
  57. ncbi Preventing phage lysis of Lactococcus lactis in cheese production using a neutralizing heavy-chain antibody fragment from llama
    A M Ledeboer
    Unilever Research and Development Vlaardingen, The Netherlands
    J Dairy Sci 85:1376-82. 2002
    ..Moreover the antibodies are produced in a food grade microorganism and can easily be isolated from the fermentation liquid in a pure and DNA free form...
  58. pmc Mucosal vaccine made from live, recombinant Lactococcus lactis protects mice against pharyngeal infection with Streptococcus pyogenes
    Praveen Mannam
    Department of Microbiology, Oregon State University, Corvallis, Oregon 97330 3804, USA
    Infect Immun 72:3444-50. 2004
    A novel vaccine (LL-CRR) made from live, nonpathogenic Lactococcus lactis that expresses the conserved C-repeat region (CRR) of M protein from Streptococcus pyogenes serotype 6 was tested in mice...
  59. pmc Molecular physiology of sugar catabolism in Lactococcus lactis IL1403
    S Even
    , UMR 5504 INSA/CNRS and UMR 792 INSA/INRA, , 31077 Toulouse Cedex 4, France
    J Bacteriol 183:3817-24. 2001
    The metabolic characteristics of Lactococcus lactis IL1403 were examined on two different growth media with respect to the physiological response to two sugars, glucose and galactose...
  60. pmc Design of a protein-targeting system for lactic acid bacteria
    Y Dieye
    , INRA, 78352 Jouy-en-Josas Cedex, France
    J Bacteriol 183:4157-66. 2001
    ..that enabled the targeting of a reporter protein (the staphylococcal nuclease Nuc) to specific locations in Lactococcus lactis cells, i.e., cytoplasm, cell wall, or medium...
  61. pmc Growth rate regulated genes and their wide involvement in the Lactococcus lactis stress responses
    Clémentine Dressaire
    Universite de Toulouse INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F 31077 Toulouse, France
    BMC Genomics 9:343. 2008
    ..The exclusive growth rate response can be achieved through chemostat cultivation, enabling all parameters to remain constant except the growth rate...
  62. ncbi Inactivation of a gene that is highly conserved in Gram-positive bacteria stimulates degradation of non-native proteins and concomitantly increases stress tolerance in Lactococcus lactis
    D Frees
    Department of Veterinary Microbiology, Stigbøjlen 4, Denmark
    Mol Microbiol 41:93-103. 2001
    ..major role in stress tolerance and in the degradation of non-native proteins in the Gram-positive bacterium Lactococcus lactis. Here, we used transposon mutagenesis to generate mutants in which the temperature- and puromycin-sensitive ..
  63. pmc A food-grade approach for functional analysis and modification of native plasmids in Lactococcus lactis
    Paul D Cotter
    Department of Microbiology and National Food Biotechnology Centre, University College Cork, Cork, Ireland
    Appl Environ Microbiol 69:702-6. 2003
    ..2-kb plasmid pMRC01 and to select for the transfer of pMRC01 between Lactococcus lactis strains.
  64. ncbi Oral immunization with live Lactococcus lactis expressing rotavirus VP8 subunit induces specific immune response in mice
    Belkis Marelli
    Instituto de Biología Molecular y Celular de Rosario IBR CONICET and Departamento de Microbiología, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina
    J Virol Methods 175:28-37. 2011
    ..The development of a mucosal live vaccine using the food-grade lactic acid bacteria Lactococcus lactis as antigen vehicle is an attractive and safe vaccination strategy against rotavirus...
  65. pmc Complete genome sequence of Lactococcus lactis subsp. cremoris A76
    Alexander Bolotin
    INRA, AgroParisTech, UMR1319 MICALIS, Jouy en Josas, France
    J Bacteriol 194:1241-2. 2012
    We report the complete genome sequence of Lactococcus lactis subsp. cremoris A76, a dairy strain isolated from a cheese production outfit. Genome analysis detected two contiguous islands fitting to the L. lactis subsp...
  66. pmc GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactis
    Rasmus Larsen
    Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    J Bacteriol 188:4978-82. 2006
    We show that the nitrogen regulatory protein GlnR of Lactococcus lactis represses transcription of the amtB-glnK, glnRA, and glnPQ operons...
  67. ncbi Microbiological and molecular impacts of AbiK on the lytic cycle of Lactococcus lactis phages of the 936 and P335 species
    I Boucher
    Department of Biochemistry and Microbiology, Faculte des sciences et de genie, Groupe de Recherche en Ecologie Buccale GREB, Faculte de Medecine Dentaire, Universite Laval, Quebec, Canada
    Microbiology 146:445-53. 2000
    ..To the knowledge of the authors, this is the first time that different phage responses towards an Abi system have been reported...
  68. pmc DNA Macroarray profiling of Lactococcus lactis subsp. lactis IL1403 gene expression during environmental stresses
    Yi Xie
    Department of Nutrition and Food Sciences, Utah State University, Logan, Utah 84322 4700, USA
    Appl Environ Microbiol 70:6738-47. 2004
    This report describes the use of an oligonucleotide macroarray to profile the expression of 375 genes in Lactococcus lactis subsp. lactis IL1403 during heat, acid, and osmotic stress...
  69. pmc Bioengineering of a Nisin A-producing Lactococcus lactis to create isogenic strains producing the natural variants Nisin F, Q and Z
    Clare Piper
    Department of Microbiology, University College Cork, College Road, Cork, Ireland
    Microb Biotechnol 4:375-82. 2011
    ..Nisins A, Z, F and Q are produced by Lactococcus lactis, while Nisins U and U2 are produced by Streptococcus sp...
  70. ncbi Sequencing of the tyrosine decarboxylase cluster of Lactococcus lactis IPLA 655 and the development of a PCR method for detecting tyrosine decarboxylating lactic acid bacteria
    Maria Fernandez
    Instituto de Productos Lacteos de Asturias, Carretera de Infiesto s n, 33300 Villaviciosa, Asturias, Spain
    J Food Prot 67:2521-9. 2004
    ..The Lactococcus lactis strain IPLA 655 was selected, and the genes encoding a putative tyrosyl tRNA synthetase, a tyrosine ..
  71. pmc Lactococcus lactis NCC 2287 alleviates food allergic manifestations in sensitized mice by reducing IL-13 expression specifically in the ileum
    Adrian W Zuercher
    Allergy Group, Department of Nutrition and Health, Nestle Research Center, Vers chez les Blanc, 1000 Lausanne 26, Switzerland
    Clin Dev Immunol 2012:485750. 2012
    Utilizing a food allergy murine model, we have investigated the intrinsic antiallergic potential of the Lactococcus lactis NCC 2287 strain.
  72. pmc Evaluation of oral immunization with recombinant avian influenza virus HA1 displayed on the Lactococcus lactis surface and combined with the mucosal adjuvant cholera toxin subunit B
    Han Lei
    College of Life Science and Food Engineering, Nanchang University, Nanchang 330013, People s Republic of China
    Clin Vaccine Immunol 18:1046-51. 2011
    ..In this study, we constructed a recombinant (pgsA-HA1 gene fusion) Lactococcus lactis strain that expresses and displays the avian influenza virus HA1 antigens on its surface...
  73. ncbi Protection against tetanus toxin after intragastric administration of two recombinant lactic acid bacteria: impact of strain viability and in vivo persistence
    Corinne Grangette
    Département de Microbiologie des Ecosytèmes, Institut Pasteur de Lille, 59019 Lille Cedex, France
    Vaccine 20:3304-9. 2002
    ..this question, recombinant LAB strains, able to persist (Lactobacillus plantarum NCIMB8826/pMEC127) or not (Lactococcus lactis MG1363/pMEC46) in the gastro-intestinal tract of mice and producing equivalent amounts of the tetanus toxin ..
  74. ncbi DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanisms
    I Boucher
    Department of Biochemistry and Microbiology, Faculte des sciences et de genie, Faculte de Medecine Dentaire, Universite Laval, Quebec, Canada
    J Dairy Sci 84:1610-20. 2001
    The three Lactococcus lactis plasmids pSRQ700, pSRQ800, and pSRQ900 encode the previously described anti-phage resistance mechanisms LlaDCHI, AbiK, and AbiQ, respectively...
  75. ncbi Expression of bioactive porcine interferon-gamma by recombinant Lactococcus lactis
    Prithy Rupa
    Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Vet Microbiol 129:197-202. 2008
    b>Lactococcus lactis is an immunomodulator and candidate live mucosal delivery vehicle for vaccine antigens and for biologically active molecules, including immunoregulatory cytokines such as interferon-gamma (IFN-gamma)...
  76. ncbi Expression of mptC of Listeria monocytogenes induces sensitivity to class IIa bacteriocins in Lactococcus lactis
    Manilduth Ramnath
    Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa
    Microbiology 150:2663-8. 2004
    ..activity, the mptACD operon, encoding, was heterologously expressed in an insensitive species, namely Lactococcus lactis, using the NICE double plasmid system. Upon induction of the cloned operon, the recombinant Lc...
  77. ncbi RNA extraction from cheese for analysis of in situ gene expression of Lactococcus lactis
    V M Ulve
    UMR1253, Science et Technologie du Lait et de l Oeuf, INRA, Rennes, France
    J Appl Microbiol 105:1327-33. 2008
    ..The isolation of high-quality RNA from cheese is a prerequisite for analysis of in situ gene expression of dairy micro-organisms...
  78. ncbi Multivitamin production in Lactococcus lactis using metabolic engineering
    Wilbert Sybesma
    Department of Flavor, Nutrition and Natural Ingredients, Wageningen Centre for Food Sciences, NIZO Food Research, Kernhemseweg 2, P O Box 20, 6710 BA Ede, The Netherlands
    Metab Eng 6:109-15. 2004
    The dairy starter bacterium Lactococcus lactis has the potential to synthesize both folate (vitamin B11) and riboflavin (vitamin B2)...
  79. ncbi Fate and efficacy of lacticin 3147-producing Lactococcus lactis in the mammalian gastrointestinal tract
    Alleson Dobson
    Teagasc, Food Research Centre Moorepark, Fermoy, Co Cork, Ireland
    FEMS Microbiol Ecol 76:602-14. 2011
    Gastrointestinal survival of the bacteriocin-producing strain, Lactococcus lactis DPC6520, was evaluated systematically in vitro and in vivo with a view to using this strain to deliver biologically active lacticin 3147, a broad-spectrum ..
  80. ncbi Modular structure of the receptor binding proteins of Lactococcus lactis phages. The RBP structure of the temperate phage TP901-1
    Silvia Spinelli
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS and Université d Aix Marseille I, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France
    J Biol Chem 281:14256-62. 2006
    b>Lactococcus lactis is a gram-positive bacterium widely used by the dairy industry. Several industrial L. lactis strains are sensitive to various distinct bacteriophages...
  81. ncbi Fibronectin-binding proteins of Staphylococcus aureus mediate activation of human platelets via fibrinogen and fibronectin bridges to integrin GPIIb/IIIa and IgG binding to the FcgammaRIIa receptor
    J Ross Fitzgerald
    Department of Microbiology, Moyne Institute of Preventive Medicine, University of Dublin, Trinity College, Dublin, Ireland
    Mol Microbiol 59:212-30. 2006
    ..aureus or Lactococcus lactis, indicating two distinct mechanisms of activation...
  82. pmc Anatomy of a lactococcal phage tail
    Stephen Mc Grath
    Department of Microbiology, National University of Ireland, Cork, Ireland
    J Bacteriol 188:3972-82. 2006
    ..Furthermore, comparisons with lambda and other lactococcal phages allowed the specification of a number of genetic submodules likely to encode specific tail structures...
  83. ncbi Construction and characterization of a Lactococcus lactis strain deficient in intracellular ClpP and extracellular HtrA proteases
    N G Cortes-Perez
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Microbiology 152:2611-8. 2006
    A Lactococcus lactis strain deficient in both its major proteases, intracellular (ClpP) and extracellular (HtrA), was constructed and characterized...
  84. pmc Identification of the leucine-to-2-methylbutyric acid catabolic pathway of Lactococcus lactis
    Balasubramanian Ganesan
    Western Dairy Center, Utah State University, Logan, UT 84322 4700, USA
    Appl Environ Microbiol 72:4264-73. 2006
    ..Phylogenetic analyses showed that homologues of these enzymes and their functional motifs were widespread across the domains of life...
  85. pmc Improved stress tolerance of GroESL-overproducing Lactococcus lactis and probiotic Lactobacillus paracasei NFBC 338
    C Desmond
    Teagasc, Biotechnology Centre, Moorepark, Fermoy, County Cork, Ireland
    Appl Environ Microbiol 70:5929-36. 2004
    ..These vectors were transferred into Lactococcus lactis(pGRO1) and L...
  86. pmc Sequence analysis of the lactococcal plasmid pNP40: a mobile replicon for coping with environmental hazards
    Jonathan O'Driscoll
    Department of Microbiology, University College Cork, Western Road, Cork, Ireland
    J Bacteriol 188:6629-39. 2006
    The conjugative lactococcal plasmid pNP40, identified in Lactococcus lactis subsp...
  87. ncbi Immunogenicity and protective efficacy of orally administered recombinant Lactococcus lactis expressing surface-bound HIV Env
    Ke Qin Xin
    Department of Bacteriology, Yokohama City University School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
    Blood 102:223-8. 2003
    This study investigates whether genetically modified orally administered Lactococcus lactis (L lactis) could be used as an HIV vaccine. L lactis is immunogenic and extremely safe when delivered orally...
  88. pmc Carbohydrate starvation causes a metabolically active but nonculturable state in Lactococcus lactis
    Balasubramanian Ganesan
    Center for Integrated BioSystems, Utah State University, Logan, UT 84322 4700, USA
    Appl Environ Microbiol 73:2498-512. 2007
    ....
  89. ncbi Paracellular entry of interleukin-10 producing Lactococcus lactis in inflamed intestinal mucosa in mice
    Anouk Waeytens
    Department of Pathology, Ghent University, Ghent, Belgium
    Inflamm Bowel Dis 14:471-9. 2008
    Genetically modified Lactococcus lactis secreting interleukin-10 (IL-10) has been demonstrated to provide localized delivery of a therapeutic agent through active in situ synthesis in murine colitis...
  90. ncbi Lactococcus lactis as a live vector for mucosal delivery of therapeutic proteins
    Luis G Bermúdez-Humarán
    Equipe Interactions des bactéries commensales et probiotiques avec l hôte, Unité d Ecologie et Physiologie du Système Digestif, INRA 0910, Jouy en Josas, France
    Hum Vaccin 5:264-7. 2009
    Food-grade lactic acid bacteria (LAB) have been safely consumed by humans for centuries in fermented foods. Lactococcus lactis, a LAB widely used in the dairy industry as a starter, can be genetically engineered to efficiently produce a ..
  91. ncbi Identification of tet(M) in two Lactococcus lactis strains isolated from a Spanish traditional starter-free cheese made of raw milk and conjugative transfer of tetracycline resistance to lactococci and enterococci
    Ana Belén Flórez
    Instituto de Productos Lácteos de Asturias CSIC, Carretera de Infiesto s n, 33300 Villaviciosa, Asturias, Spain
    Int J Food Microbiol 121:189-94. 2008
    Specific PCR and sequencing showed that a tet(M) gene was present in two tetracycline-resistant Lactococcus lactis strains isolated from a raw milk, starter-free cheese...
  92. pmc Complete genome sequence of Lactococcus lactis subsp. lactis KF147, a plant-associated lactic acid bacterium
    Roland J Siezen
    NIZO Food Research, Kluyver Centre for Genomics of Industrial Fermentation, P O Box 20, 6710 BA Ede, The Netherlands
    J Bacteriol 192:2649-50. 2010
    b>Lactococcus lactis is a lactic acid bacterium used in the production of many fermented dairy products. We report the complete genome sequence of L. lactis subsp. lactis KF147, a nondairy strain isolated from mung bean sprouts...
  93. pmc Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis
    Ravi K R Marreddy
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
    PLoS ONE 5:e10317. 2010
    ..In this study, we employed a proteomic approach to understand the difference in expression levels for various multidomain membrane proteins in L. lactis cells grown in complex and synthetic media...
  94. ncbi Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins
    Daniel M Linares
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Mol Biol 401:45-55. 2010
    ..The generality and simplicity of our approach allow rapid improvements of protein production yields by directed evolution in a high-throughput way...
  95. pmc Bovine rotavirus nonstructural protein 4 produced by Lactococcus lactis is antigenic and immunogenic
    V Enouf
    , INRA, 78352 Jouy en Josas, France
    Appl Environ Microbiol 67:1423-8. 2001
    ..The capacity of the generally regarded as safe organism Lactococcus lactis to produce NSP4 either intra- or extracellularly was then investigated by using the nisin-controlled ..
  96. pmc Multi-omics approach to study the growth efficiency and amino acid metabolism in Lactococcus lactis at various specific growth rates
    Petri Jaan Lahtvee
    Tallinn University of Technology, Department of Chemistry, Akadeemia tee 15, 12618 Tallinn, Estonia
    Microb Cell Fact 10:12. 2011
    b>Lactococcus lactis is recognised as a safe (GRAS) microorganism and has hence gained interest in numerous biotechnological approaches...
  97. pmc Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis
    J J Godon
    Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
    J Bacteriol 175:4383-90. 1993
    The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs...
  98. ncbi Proteome phenotyping of acid stress-resistant mutants of Lactococcus lactis MG1363
    Aurélie Budin-Verneuil
    Laboratoire Microbiologie de l Environnement, EA956 USC INRA 2017, Universite de Caen, Caen, France
    Proteomics 7:2038-46. 2007
    To cope with medium acidity, Lactococcus lactis has evolved a number of inducible mechanisms commonly referred as acid stress response...
  99. pmc Gene inactivation in Lactococcus lactis: histidine biosynthesis
    C Delorme
    Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
    J Bacteriol 175:4391-9. 1993
    b>Lactococcus lactis strains from dairy and nondairy sources were tested for the ability to grow in the absence of histidine. Among 60 dairy strains tested, 56 required histidine, whereas only 1 of 11 nondairy strains had this requirement...
  100. ncbi Genetically engineered Lactococcus lactis secreting murine IL-10 modulates the functions of bone marrow-derived dendritic cells in the presence of LPS
    M Loos
    Department for Molecular Biomedical Research, VIB, Ghent, Belgium
    Scand J Immunol 69:130-9. 2009
    Oral delivery of IL-10 by genetically modified Lactococcus lactis (LL-pTmIL10) has been shown to efficiently reduce intestinal inflammation in mice with chronic colitis, but the mechanisms involved have not been elucidated...
  101. ncbi Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion
    E Morello
    Unité des Bactéries Lactiques et pathogènes Opportunistes UBLO, INRA, Jouy en Josas, France
    J Mol Microbiol Biotechnol 14:48-58. 2008
    ..The lactic acid bacterium Lactococcus lactis emerges as an attractive alternative to the Gram-positive model Bacillus subtilis...

Research Grants26

  1. A. L. lactis-based vaccine for children with broad spectrum for enteric pathogens
    Wendy L Picking; Fiscal Year: 2013
    ..We will explore a novel platform technology consisting of nonliving, non-genetically modified, Lactococcus lactis particles, designated Gram-positive Enhancer Matrix (GEM) that will be engineered to display Type III ..
  2. Lipid Translocating Glycosyltransferases in Congenital Neurological Disease
    PETER AB ORLEAN; Fiscal Year: 2010
    ..Proteins will first be expressed in the Gram positive bacterium Lactococcus lactis, which targets polytopic membrane proteins to its single membrane...
  3. INTRON DYNAMICS IN BACTERIA
    Marlene Belfort; Fiscal Year: 2013
    ..previous funding period, as follows: First, we will further explore our findings that dissemination of the Lactococcus lactis group II intron, Ll...
  4. Structural Dynamics of Drug/Proton Antiport by a Multidrug Efflux Pump
    PHILLIP RYAN STEED; Fiscal Year: 2013
    ..Biochemical studies of two representatives of the DHA family, LmrP from Lactococcus lactis and MdfA from Escherichia coli, have shed light on the substrate specificity and the transport mechanism...
  5. Involvement of Proteins in Splicing Group I and II Introns
    Alan M Lambowitz; Fiscal Year: 2013
    ..For group II introns, we have used the Lactococcus lactis Ll...
  6. Non-peptidic HIV vaccine
    Miroslav Malkovsky; Fiscal Year: 2010
    ..purpose we have developed highly immunogenic recombinant attenuated Listeria vaccine vectors and recombinant Lactococcus lactis vaccine particles...
  7. Analysis of Group I and II Introns in Mammalian Cells
    Bruce Sullenger; Fiscal Year: 2005
    ..More recently, we demonstrated that the Lactococcus lactis group II intron can reverse-splice and insert itself into DNA in transfected human cells...
  8. SLS: Molecular Basis and Role in Invasive GAS Disease
    Victor Nizet; Fiscal Year: 2006
    ..for GAS SLS production and sufficient to confer SLS activity to the nonpathogenic heterologous species Lactococcus lactis. Sequence features and homologies strongly suggest SLS belongs to the bacteriocin class of toxins, with sagA ..
  9. C3-Binding and -Degrading Proteins in S. pneumoniae
    MARGARET HOSTETTER; Fiscal Year: 2002
    ..Truncation constructs expressed in Lactococcus lactis will be used to map the active site. A cppA- mutant in an encapsulated serotype 4 will be constructed...
  10. DISRUPTING VIRAL GENES WITH GROUP II INTRONS
    Bruce Sullenger; Fiscal Year: 2004
    ..Mobility of the Lactococcus lactis group II intron occurs through a two-step process...
  11. SUMO Fusions to Enhance Expression and Secretion of Protiens
    TAUSEEF BUTT; Fiscal Year: 2007
    ..coli. Recognizing the well-known protein secretion properties of Bacillus subtilis and Lactococcus lactis we also proposed to test the role of N-terminal of SUMO in secretion of protein in these gram-positive ..
  12. Mechanism of Group II Intron Retrotransposition
    LORI CONLAN; Fiscal Year: 2005
    ..overall goal of this project is to combine genetic, biochemical and structural studies to characterize the Lactococcus lactis group II intron-encoded protein (LtrA) and the pathways involved in intron mobility, particularly ..
  13. Cellular Osmoregulation:Sensors, Transducers& Regulators
    Janet Wood; Fiscal Year: 2003
    ..is proposed since it supports prominent conference participants who study mechanisms that allow kidney cells to thrive despite the fluctuating osmolality, salinity and urea concentration associated with the renal concentrating mechanism ..
  14. Lactococcal vaccine for strep throat
    Bruce Geller; Fiscal Year: 2004
    ..The immediate, short term goals to be accomplished in phase I are: 1) Optimize surface expression in Lactococcus lactis of the conserved C-repeat region (CRR) of the M6 protein of Streptococcus pyogenes; 2) Optimize vaccine dose ..
  15. Flavoenzymes in Pyrimidine Metabolism
    Bruce A Palfey; Fiscal Year: 2011
    ..Our studies of the rates of the chemical reactions will identify important parts of the proteins, how they move during reactions, how they speed the synthesis of products, and how these reactions might be blocked. ..
  16. A BACTERIAL GROUP II INTRON INVOLVED IN CONJUGATION
    Gary Dunny; Fiscal Year: 2002
    ..PI in the course of collaborative research with the coinvestigator on conjugative plasmid transfer of the Lactococcus lactis plasmid pRSO1...
  17. MOLECULAR SEQUENCE DATA
    Samuel Karlin; Fiscal Year: 2001
    ..by spacings between repeats, and by properties of repeat families (intergenic, coding, direct, inverted, mixed). ..
  18. Evolutionary Genomics of Anti-Malaria Genes in Mosquito
    Brian P Lazzaro; Fiscal Year: 2010
    ..Evolutionary patterns in mosquito defense genes will be used to identify proteins that coevolve with malaria parasites. This work may suggest genetic targets that could be manipulated to limit malaria transmission. ..
  19. Exocyclic C-C Modifications of Aromatic Polyketides
    JON THORSON; Fiscal Year: 2008
    ..A particular focus of the final diversification strategies presented will be focused upon conjugation of these highly reactive antitumor antibiotics with known tumor-directing peptides. ..
  20. Virulence-conferring siderophore biosynthesis inhibitors
    LUIS QUADRI; Fiscal Year: 2006
    ..These antimicrobials will constitute a base for subsequent studies for the development of new drugs that, alone or in combination therapies, are anticipated to be useful in the treatment of MDR Yp and Mb infections. ..
  21. Cell Wall Components' Role in Enterococcal Endocarditis
    Gary Dunny; Fiscal Year: 2006
    ..faecalis virulence. 2) Determine the functional domains of Asc10 that confer the activities identified in Aim 1. 3) Examine the expression of bacterial and host genes during the course of infections. ..
  22. Gene Expression Levels Across Diverse Genomes
    Samuel Karlin; Fiscal Year: 2006
    ..Staphylococcus aureus, Streptococcus pyogenes, and the nonpathogenic dairy fermentation bacterium Lactococcus lactis. The second group consists of all available high G+C a-proteobacteria...
  23. LETHAL OROESOPHAGEAL CANDIDIASIS: IMMUNOTHERAPY
    Edward Balish; Fiscal Year: 2004
    ..Restoration and enhancement of immune function may be critical for the effectiveness of anti-Candida vaccines that are now under development. ..
  24. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2002
    ....
  25. GENETIC ANALYSIS OF GBS ADHERENCE TO FIBRONECTIN
    GLEN TAMURA; Fiscal Year: 2002
    ..These studies will allow for identification of the structural gene(s) for fibronectin adhesin(s). ..
  26. Analysis of Group B Streptococcal Fibrinogen Cleavage
    Daniel Shelver; Fiscal Year: 2005
    ..The availability of the purified protein will facilitate future biochemical structure-function studies on this important virulence factor of GBS. ..