Summary: A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.
Publications294 found, 100 shown here
- Time-resolved genetic responses of Lactococcus lactis to a dairy environmentHerwig Bachmann
NIZO Food Research, Ede, The Netherlands
Environ Microbiol 12:1260-70. 2010b>Lactococcus lactis is one of main bacterial species found in mixed dairy starter cultures for the production of semi-hard cheese...
- Phage abortive infection in lactococci: variations on a themeMarie Christine Chopin
Unité de Génétique Microbienne, INRA, CRJJ, 78352 Jouy en Josas Cedex, France
Curr Opin Microbiol 8:473-9. 2005..Twenty Abi systems have been isolated in Lactococcus lactis, a bacterium used in cheese-making fermentation processes, where phage attacks are of economical importance...
- A general system for generating unlabelled gene replacements in bacterial chromosomesK Leenhouts
Department of Genetics, University of Groningen, Haren, The Netherlands
Mol Gen Genet 253:217-24. 1996..A feasibility study was performed using Lactococcus lactis and Bacillus subtilis as model organisms...
- Identification of the receptor-binding protein in 936-species lactococcal bacteriophagesKitt Dupont
Department of Food Science, The Royal Veterinary and Agricultural University, DK 1958 Frederiksberg C, Denmark
Appl Environ Microbiol 70:5818-24. 2004..The recombinant phages were able to form plaques on the sk1 host Lactococcus lactis MG1614, and recombination was verified by PCR analysis directly with the plaques...
- Characterization of AcmB, an N-acetylglucosaminidase autolysin from Lactococcus lactisCarine Huard
Unité de Biochimie et Structure des Protéines, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
Microbiology 149:695-705. 2003..hydrolase, named acmB, which is a paralogue of the major autolysin acmA gene, was identified in the Lactococcus lactis genome sequence. The acmB gene is transcribed in L...
- Investigation of the adaptation of Lactococcus lactis to isoleucine starvation integrating dynamic transcriptome and proteome informationClémentine Dressaire
Universite de Toulouse INSA, UPS, INP LISBP, 135 Avenue de Rangueil, F 31077 Toulouse, France
Microb Cell Fact 10:S18. 2011..The global response of the LAB model Lactococcus lactis ssp...
- Peptidoglycan structure analysis of Lactococcus lactis reveals the presence of an L,D-carboxypeptidase involved in peptidoglycan maturationPascal Courtin
INRA, Unité de Biochimie Bactérienne, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
J Bacteriol 188:5293-8. 2006Detailed structural analysis of Lactococcus lactis peptidoglycan was achieved by identification of its constituent muropeptides separated by reverse phase high-performance liquid chromatography...
- Overview on sugar metabolism and its control in Lactococcus lactis - the input from in vivo NMRAna Rute Neves
Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Rua da Quinta Grande, 6, Apt 127, 2780 156 Oeiras, Portugal
FEMS Microbiol Rev 29:531-54. 2005..The relative metabolic simplicity and the availability of genetic tools made Lactococcus lactis the organism of choice to gain insight into metabolic and regulatory networks...
- Genome-scale diversity and niche adaptation analysis of Lactococcus lactis by comparative genome hybridization using multi-strain arraysRoland J Siezen
Kluyver Centre for Genomics of Industrial Fermentation, NIZO Food Research, P O Box 20, 6710 BA Ede, The Netherlands
Microb Biotechnol 4:383-402. 2011b>Lactococcus lactis produces lactic acid and is widely used in the manufacturing of various fermented dairy products. However, the species is also frequently isolated from non-dairy niches, such as fermented plant material...
- Sequence and comparative genomic analysis of lactococcal bacteriophages jj50, 712 and P008: evolutionary insights into the 936 phage speciesJennifer Mahony
Department of Microbiology, University College Cork, Ireland
FEMS Microbiol Lett 261:253-61. 2006..These genetic differences may be responsible for the observed differential sensitivity to the lactococcal DNA injection blocking protein, Sie(2009), and the abortive infection system, AbiA...
- Neonatal mucosal immunization with a non-living, non-genetically modified Lactococcus lactis vaccine carrier induces systemic and local Th1-type immunity and protects against lethal bacterial infectionK Ramirez
Department of Pediatrics, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA
Mucosal Immunol 3:159-71. 2010..the capacity of a novel mucosal antigen delivery system consisting of non-living, non-genetically modified Lactococcus lactis particles, designated as Gram-positive enhancer matrix (GEM), to induce immune responses in the neonatal ..
- Effect of different NADH oxidase levels on glucose metabolism by Lactococcus lactis: kinetics of intracellular metabolite pools determined by in vivo nuclear magnetic resonanceAna Rute Neves
Instituto de Tecnologia Química e Biológica Universidade Nova de Lisboa and Instituto de Biologia Experimental e Tecnológica, 2780 156 Oeiras, Portugal
Appl Environ Microbiol 68:6332-42. 2002Three isogenic strains of Lactococcus lactis with different levels of H(2)O-forming NADH oxidase activity were used to study the effect of oxygen on glucose metabolism: the parent strain L...
- Experimental conditions affect the site of tetrazolium violet reduction in the electron transport chain of Lactococcus lactisSybille Tachon
INRA, UR 477 Biochimie Bactérienne, F 78350 Jouy en Josas, France
Microbiology 155:2941-8. 2009..The objective of the present study was to identify the reduction mechanisms of tetrazolium violet (TV) in Lactococcus lactis using a mutagenesis approach, under two experimental conditions generally applied in microbiology: a plate ..
- A Plasmodium falciparum GLURP-MSP3 chimeric protein; expression in Lactococcus lactis, immunogenicity and induction of biologically active antibodiesMichael Theisen
Department of Clinical Biochemistry, Statens Serum Institut, Copenhagen, Denmark
Vaccine 22:1188-98. 2004..falciparum Merozoite surface protein 3 (MSP3) was produced in Lactococcus lactis as a secreted recombinant GLURP-MSP3 fusion protein...
- Novel surface display system for proteins on non-genetically modified gram-positive bacteriaTjibbe Bosma
BiOMaDe Technology, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Appl Environ Microbiol 72:880-9. 2006..This binding domain, the protein anchor (PA), was derived from the Lactococcus lactis peptidoglycan hydrolase AcmA. GEM particles were typically prepared from the innocuous bacterium L...
- Prevention and treatment of colitis with Lactococcus lactis secreting the immunomodulatory Yersinia LcrV proteinBenoit Foligne
Laboratoire des Bactéries Lactiques et Immunité des Muqueuses, Institut Pasteur de Lille, Lille, France
Gastroenterology 133:862-74. 2007....
- Linear covariance models to examine the determinants of protein levels in Lactococcus lactisClémentine Dressaire
Universite de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F 31077 Toulouse, France
Mol Biosyst 6:1255-64. 2010..by statistical modelling, of the major determinants of protein concentrations in a bacterial model Lactococcus lactis. Protein concentrations were predicted by covariance models taking into account various quantitative and ..
- Conservation of key elements of natural competence in Lactococcus lactis sspSandra Wydau
Génétique Microbienne, INRA, Domaine de Vilvert, Jouy en Josas Cedex, France
FEMS Microbiol Lett 257:32-42. 2006..Recently, the genome sequence of various species, including Lactococcus lactis, revealed the presence of homologues of competence genes in bacteria, which were not previously identified ..
- The anaerobic (class III) ribonucleotide reductase from Lactococcus lactis. Catalytic properties and allosteric regulation of the pure enzyme systemE Torrents
Department of Biochemistry 1, Medical Nobel Institute, MBB, Karolinska Institutet, S 17177 Stockholm, Sweden
J Biol Chem 275:2463-71. 2000b>Lactococcus lactis contains an operon with the genes (nrdD and nrdG) for a class III ribonucleotide reductase. Strict anaerobic growth depends on the activity of these genes...
- HtrA is a key factor in the response to specific stress conditions in Lactococcus lactisCatherine Foucaud-Scheunemann
INRA, Unité de Recherches Laitières et Génétique Appliquée, 78352 Jouy en Josas, France
FEMS Microbiol Lett 224:53-9. 2003We investigated the physiological role of Lactococcus lactis housekeeping surface protease HtrA. It is involved in surface properties under regular growth conditions, as the htrA mutant strain forms longer chains in liquid medium...
- Acetate utilization in Lactococcus lactis deficient in lactate dehydrogenase: a rescue pathway for maintaining redox balanceP Hols
Microbial Ingredients Section, NIZO Food Research and Wageningen Centre for Food Science, 6710 BA Ede, The Netherlands
J Bacteriol 181:5521-6. 1999Acetate was shown to improve glucose fermentation in Lactococcus lactis deficient in lactate dehydrogenase...
- Complete sequences of four plasmids of Lactococcus lactis subsp. cremoris SK11 reveal extensive adaptation to the dairy environmentRoland J Siezen
Centre for Molecular andBiomolecular Informatics, Radboud University, P O Box 9010, 6500GL Nijmegen, The Netherlands
Appl Environ Microbiol 71:8371-82. 2005b>Lactococcus lactis strains are known to carry plasmids encoding industrially important traits. L. lactis subsp. cremoris SK11 is widely used by the dairy industry in cheese making...
- Dynamic response of catabolic pathways to autoacidification in Lactococcus lactis: transcript profiling and stability in relation to metabolic and energetic constraintsSergine Even
Laboratorie Biotechnologie Bioprocédés, UMR 5504 INSA CNRS and UMR 792 INSA INRA, Centre de Bioingenierie Gilbert Durand, Institute National des Sciences Appliquées, Toulouse, France
Mol Microbiol 45:1143-52. 2002..the central metabolic pathways to autoacidification (accumulation of organic acid fermentation products) in Lactococcus lactis was investigated in a global manner by integrating molecular data (cellular transcript concentrations, mRNA ..
- Generation and evaluation of A2-expressing Lactococcus lactis live vaccines against Leishmania donovani in BALB/c miceKaren K Yam
Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada
J Med Microbiol 60:1248-60. 2011..efficacy of Leishmania vaccines engineered from the non-colonizing, non-pathogenic Gram-positive bacterium Lactococcus lactis. A truncated, codon-optimized version of the A2 antigen from Leishmania donovani was engineered for ..
- Prophylaxis of experimentally induced ovomucoid allergy in neonatal pigs using Lactococcus lactisP Rupa
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada
Vet Immunol Immunopathol 140:23-9. 2011Probiotic Lactococcus lactis (LL) is immunomodulatory and may prevent allergy by biasing from type-2 to a type-1 immune response...
- Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403Brice Sperandio
Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
J Bacteriol 187:3762-78. 2005..for the synthesis of cysteine and methionine and their interconversion were experimentally determined for Lactococcus lactis, a lactic acid bacterium commonly found in food...
- LmrR is a transcriptional repressor of expression of the multidrug ABC transporter LmrCD in Lactococcus lactisHerfita Agustiandari
Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands
J Bacteriol 190:759-63. 2008LmrCD is an ABC-type multidrug transporter in Lactococcus lactis. LmrR encodes a putative transcriptional regulator. In a DeltalmrR strain, lmrCD is up-regulated...
- Effect of exopolysaccharides on phage-host interactions in Lactococcus lactisHélène Deveau
Departement de Biochimie et de Microbiologie, Faculte des sciences et de genie, Groupe de Recherche en Ecologie Buccale GREB, Faculte de Medecine Dentaire, Universite Laval, Quebec, Canada G1K 7P4
Appl Environ Microbiol 68:4364-9. 2002In this study, we report that Lactococcus lactis strains producing exopolysaccharides (EPS) are sensitive to virulent phages...
- Complete genome sequence of Lactococcus lactis subsp. lactis CV56, a probiotic strain isolated from the vaginas of healthy womenYong Gao
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No 1 Beichen West Road, Chaoyang District, Beijing 100101, People s Republic of China
J Bacteriol 193:2886-7. 2011..Here, we report the finished and annotated genome of a Lactococcus strain that was isolated from the vaginas of healthy women and shows probiotic properties, including nisin A production and adhesion to vaginal epithelial cells...
- Microbial domestication signatures of Lactococcus lactis can be reproduced by experimental evolutionHerwig Bachmann
NIZO Food Research, 6710BA Ede, The Netherlands
Genome Res 22:115-24. 2012..The lactic acid bacterium Lactococcus lactis naturally occurs on plants and in dairy environments, and it is proposed that dairy strains originate from ..
- Population genomics and phylogeography of an Australian dairy factory derived lytic bacteriophageEduardo Castro-Nallar
Department of Biology, Brigham Young University
Genome Biol Evol 4:382-93. 2012..we present the full genomic sequences and evolutionary analyses of a serially sampled population of 28 Lactococcus lactis-infecting phage belonging to the 936-like group in Australia...
- A transcriptional activator, homologous to the Bacillus subtilis PurR repressor, is required for expression of purine biosynthetic genes in Lactococcus lactisM Kilstrup
Department of Microbiology, Technical University of Denmark, DK2800 Lyngby, Denmark
J Bacteriol 180:3907-16. 1998A purR::pGh9:ISS1 mutant of Lactococcus lactis was obtained following transposon mutagenesis of strain MG1363 and selection for purine auxotrophs...
- Overall control of nitrogen metabolism in Lactococcus lactis by CodY, and possible models for CodY regulation in FirmicutesEric Guedon
Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
Microbiology 151:3895-909. 2005..species of Gram-positive bacteria, was previously described to be the central regulator of proteolysis in Lactococcus lactis. In this study, over 100 potential CodY targets were identified by DNA-microarray analysis...
- Genome sequences of Lactococcus lactis MG1363 (revised) and NZ9000 and comparative physiological studiesDaniel M Linares
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands
J Bacteriol 192:5806-12. 2010b>Lactococcus lactis NZ9000 and its parent MG1363 are the most commonly used lactic acid bacteria for expression and physiological studies. We noted unexpected but significant differences in the growth behaviors of both strains...
- Oral administration of an IL-10-secreting Lactococcus lactis strain prevents food-induced IgE sensitizationChristophe P Frossard
University Hospital of Geneva, Department of Pediatrics, Geneva, Switzerland
J Allergy Clin Immunol 119:952-9. 2007..Because tolerance to food is potentially modulated by IL-10, strategies to prevent food allergy should favor an increased delivery of IL-10 to the gut...
- Induction of heat shock proteins DnaK, GroEL, and GroES by salt stress in Lactococcus lactisM Kilstrup
Department of Microbiology, Technical University of Denmark, Lyngby, Denmark
Appl Environ Microbiol 63:1826-37. 1997The bacterium Lactococcus lactis has become a model organism in studies of growth physiology and membrane transport, as a result of its simple fermentative metabolism...
- A topological model of the baseplate of lactococcal phage Tuc2009Giuliano Sciara
Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS, 163 Avenue de Luminy Case 932, Marseille Cedex 09, France
J Biol Chem 283:2716-23. 2008Phages infecting Lactococcus lactis, a Gram-positive bacterium, are a recurrent problem in the dairy industry...
- Intranasal delivery of influenza subunit vaccine formulated with GEM particles as an adjuvantVinay Saluja
Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, 9713 AV, Groningen, The Netherlands
AAPS J 12:109-16. 2010..use of a safe adjuvant Gram-positive enhancer matrix (GEM) particles derived from the food-grade bacterium Lactococcus lactis for i.n. vaccination with subunit influenza vaccine in mice...
- Examination of post-transcriptional regulations in prokaryotes by integrative biologyFlora Picard
Universite de Toulouse, INSA, UPS, INP, LISBP, 135, Avenue de Rangueil, 31077 Toulouse, France
C R Biol 332:958-73. 2009..In this review, we report mechanisms involved in mRNA and protein stability and translation regulation in prokaryotes, and their dependence on growth phase and environmental growth conditions is particularly highlighted...
- Complete genome sequence of the prototype lactic acid bacterium Lactococcus lactis subsp. cremoris MG1363Udo Wegmann
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
J Bacteriol 189:3256-70. 2007b>Lactococcus lactis is of great importance for the nutrition of hundreds of millions of people worldwide. This paper describes the genome sequence of Lactococcus lactis subsp...
- Receptor-binding protein of Lactococcus lactis phages: identification and characterization of the saccharide receptor-binding siteDenise M Tremblay
Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS and Universités d Aix Marseille I and II, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France
J Bacteriol 188:2400-10. 2006Phage p2, a member of the lactococcal 936 phage species, infects Lactococcus lactis strains by binding initially to specific carbohydrate receptors using its receptor-binding protein (RBP)...
- Use of Lactococcus lactis expressing pili from group B Streptococcus as a broad-coverage vaccine against streptococcal diseaseScilla Buccato
Novartis Vaccines, 1 53100 Siena, Italy
J Infect Dis 194:331-40. 2006..Here, we show that GBS pilin island 1, transferred into the nonpathogenic microorganism Lactococcus lactis, leads to pilus assembly...
- Mucosal co-application of lactic acid bacteria and allergen induces counter-regulatory immune responses in a murine model of birch pollen allergyAndreas Repa
Department of Pathophysiology, Medical School AKH EBO3Q, University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
Vaccine 22:87-95. 2003..In this study, we aimed at evaluating the immunomodulatory potential of two LAB strains, Lactococcus lactis and Lactobacillus plantarum, for prophylaxis and therapy of allergic immune responses...
- Structure of lactococcal phage p2 baseplate and its mechanism of activationGiuliano Sciara
Architecture et Fonction des Macromolecules Biologiques, UMR 6098 Centre National de la Recherche Scientifique and Universités d Aix Marseille I and II, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France
Proc Natl Acad Sci U S A 107:6852-7. 2010..All known siphophages infecting gram+ Lactococcus lactis possess a baseplate at the tip of their tail involved in host recognition and attachment...
- Molecular characterization of the plasmid-encoded eps gene cluster essential for exopolysaccharide biosynthesis in Lactococcus lactisR van Kranenburg
Department of Biophysical Chemistry, NIZO, Ede, The Netherlands
Mol Microbiol 24:387-97. 1997b>Lactococcus lactis strain NIZO B40 produces an extracellular phosphopolysaccharide containing galactose, glucose, and rhamnose...
- 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactisIgor Mierau
NIZO Food Research, P O Box 20, 6710, Ede, The Netherlands
Appl Microbiol Biotechnol 68:705-17. 2005b>Lactococcus lactis is a Gram-positive lactic acid bacterium that, in addition to its traditional use in food fermentations, is increasingly used in modern biotechnological applications...
- Genome2D: a visualization tool for the rapid analysis of bacterial transcriptome dataRichard J S Baerends
Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
Genome Biol 5:R37. 2004..The compiled version of Genome2D is freely available for academic or non-profit use from http://molgen.biol.rug.nl/molgen/research/molgensoftware.php...
- Evolution of Lactococcus lactis phages within a cheese factoryGeneviève M Rousseau
Departement de Biochimie et de Microbiologie, Groupe de Recherche en Ecologie Buccale, Felix d Herelle Reference Center for Bacterial Viruses, Universite Laval, Quebec G1V 0A6, Canada
Appl Environ Microbiol 75:5336-44. 2009..These six phages infected the same two industrial Lactococcus lactis strains out of 30 tested...
- Lactococcus lactis expressing either Staphylococcus aureus fibronectin-binding protein A or Listeria monocytogenes internalin A can efficiently internalize and deliver DNA in human epithelial cellsSilvia Innocentin
Unite de Virologie et Immunologie Moleculaires, UR892 INRA, Domaine de Vilvert, F78352 Jouy en Josas Cedex, France
Appl Environ Microbiol 75:4870-8. 2009..enhanced green fluorescent protein (eGFP) expression plasmid vector was delivered in epithelial cells by Lactococcus lactis producing Listeria monocytogenes internalin A (L...
- High-throughput cloning and expression in recalcitrant bacteriaEric R Geertsma
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
Nat Methods 4:705-7. 2007..We demonstrated VBEx proof of principle for Lactococcus lactis, but the method can be adapted to all organisms for which plasmids are available.
- Peptidoglycan N-acetylglucosamine deacetylation decreases autolysis in Lactococcus lactisMickael Meyrand
INRA, Unité de Biochimie Bactérienne, UR477, 78350 Jouy en Josas, France
Microbiology 153:3275-85. 2007The gene xynD (renamed pgdA) of Lactococcus lactis IL1403 was shown to encode a peptidoglycan N-acetylglucosamine deacetylase. Inactivation of pgdA in L...
- Genome-scale genotype-phenotype matching of two Lactococcus lactis isolates from plants identifies mechanisms of adaptation to the plant nicheRoland J Siezen
NIZO Food Research, P O Box 20, 6710 BA Ede, The Netherlands
Appl Environ Microbiol 74:424-36. 2008b>Lactococcus lactis is a primary constituent of many starter cultures used for the manufacturing of fermented dairy products, but the species also occurs in various nondairy niches such as (fermented) plant material...
- Is the glycolytic flux in Lactococcus lactis primarily controlled by the redox charge? Kinetics of NAD(+) and NADH pools determined in vivo by 13C NMRAna Rute Neves
Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
J Biol Chem 277:28088-98. 2002The involvement of nicotinamide adenine nucleotides (NAD(+), NADH) in the regulation of glycolysis in Lactococcus lactis was investigated by using (13)C and (31)P NMR to monitor in vivo the kinetics of the pools of NAD(+), NADH, ATP, ..
- Genetic manipulation of Lactococcus lactis by using targeted group II introns: generation of stable insertions without selectionCourtney L Frazier
Department of Viticulture and Enology, University of California at Davis, Davis, California 95616 8749, USA
Appl Environ Microbiol 69:1121-8. 2003..efficiently into genes encoding malate decarboxylase (mleS) and tetracycline resistance (tetM) within the Lactococcus lactis genome. Integrants were readily identified and maintained in the absence of a selectable marker...
- Treatment of murine colitis by Lactococcus lactis secreting interleukin-10L Steidler
Department of Molecular Biology, Ghent University and Flanders Interuniversity Institute for Biotechnology, K L Ledeganckstraat 35, 9000 Gent, Belgium
Science 289:1352-5. 2000..Intragastric administration of IL-10-secreting Lactococcus lactis caused a 50% reduction in colitis in mice treated with dextran sulfate sodium and prevented the onset of ..
- Mucosal vaccine delivery of antigens tightly bound to an adjuvant particle made from food-grade bacteriaMaarten L van Roosmalen
BiOMaDe Technology Foundation, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Methods 38:144-9. 2006..This binding domain, the protein anchor (PA), is derived from the Lactococcus lactis AcmA cell-wall hydrolase, and contains three repeats of a LysM-type cell-wall binding motif...
- Preventing phage lysis of Lactococcus lactis in cheese production using a neutralizing heavy-chain antibody fragment from llamaA M Ledeboer
Unilever Research and Development Vlaardingen, The Netherlands
J Dairy Sci 85:1376-82. 2002..Moreover the antibodies are produced in a food grade microorganism and can easily be isolated from the fermentation liquid in a pure and DNA free form...
- Mucosal vaccine made from live, recombinant Lactococcus lactis protects mice against pharyngeal infection with Streptococcus pyogenesPraveen Mannam
Department of Microbiology, Oregon State University, Corvallis, Oregon 97330 3804, USA
Infect Immun 72:3444-50. 2004A novel vaccine (LL-CRR) made from live, nonpathogenic Lactococcus lactis that expresses the conserved C-repeat region (CRR) of M protein from Streptococcus pyogenes serotype 6 was tested in mice...
- Molecular physiology of sugar catabolism in Lactococcus lactis IL1403S Even
, UMR 5504 INSA/CNRS and UMR 792 INSA/INRA, , 31077 Toulouse Cedex 4, France
J Bacteriol 183:3817-24. 2001The metabolic characteristics of Lactococcus lactis IL1403 were examined on two different growth media with respect to the physiological response to two sugars, glucose and galactose...
- Design of a protein-targeting system for lactic acid bacteriaY Dieye
, INRA, 78352 Jouy-en-Josas Cedex, France
J Bacteriol 183:4157-66. 2001..that enabled the targeting of a reporter protein (the staphylococcal nuclease Nuc) to specific locations in Lactococcus lactis cells, i.e., cytoplasm, cell wall, or medium...
- Growth rate regulated genes and their wide involvement in the Lactococcus lactis stress responsesClémentine Dressaire
Universite de Toulouse INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F 31077 Toulouse, France
BMC Genomics 9:343. 2008..The exclusive growth rate response can be achieved through chemostat cultivation, enabling all parameters to remain constant except the growth rate...
- Inactivation of a gene that is highly conserved in Gram-positive bacteria stimulates degradation of non-native proteins and concomitantly increases stress tolerance in Lactococcus lactisD Frees
Department of Veterinary Microbiology, Stigbøjlen 4, Denmark
Mol Microbiol 41:93-103. 2001..major role in stress tolerance and in the degradation of non-native proteins in the Gram-positive bacterium Lactococcus lactis. Here, we used transposon mutagenesis to generate mutants in which the temperature- and puromycin-sensitive ..
- A food-grade approach for functional analysis and modification of native plasmids in Lactococcus lactisPaul D Cotter
Department of Microbiology and National Food Biotechnology Centre, University College Cork, Cork, Ireland
Appl Environ Microbiol 69:702-6. 2003..2-kb plasmid pMRC01 and to select for the transfer of pMRC01 between Lactococcus lactis strains.
- Oral immunization with live Lactococcus lactis expressing rotavirus VP8 subunit induces specific immune response in miceBelkis Marelli
Instituto de Biología Molecular y Celular de Rosario IBR CONICET and Departamento de Microbiología, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina
J Virol Methods 175:28-37. 2011..The development of a mucosal live vaccine using the food-grade lactic acid bacteria Lactococcus lactis as antigen vehicle is an attractive and safe vaccination strategy against rotavirus...
- Complete genome sequence of Lactococcus lactis subsp. cremoris A76Alexander Bolotin
INRA, AgroParisTech, UMR1319 MICALIS, Jouy en Josas, France
J Bacteriol 194:1241-2. 2012We report the complete genome sequence of Lactococcus lactis subsp. cremoris A76, a dairy strain isolated from a cheese production outfit. Genome analysis detected two contiguous islands fitting to the L. lactis subsp...
- GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactisRasmus Larsen
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
J Bacteriol 188:4978-82. 2006We show that the nitrogen regulatory protein GlnR of Lactococcus lactis represses transcription of the amtB-glnK, glnRA, and glnPQ operons...
- Microbiological and molecular impacts of AbiK on the lytic cycle of Lactococcus lactis phages of the 936 and P335 speciesI Boucher
Department of Biochemistry and Microbiology, Faculte des sciences et de genie, Groupe de Recherche en Ecologie Buccale GREB, Faculte de Medecine Dentaire, Universite Laval, Quebec, Canada
Microbiology 146:445-53. 2000..To the knowledge of the authors, this is the first time that different phage responses towards an Abi system have been reported...
- DNA Macroarray profiling of Lactococcus lactis subsp. lactis IL1403 gene expression during environmental stressesYi Xie
Department of Nutrition and Food Sciences, Utah State University, Logan, Utah 84322 4700, USA
Appl Environ Microbiol 70:6738-47. 2004This report describes the use of an oligonucleotide macroarray to profile the expression of 375 genes in Lactococcus lactis subsp. lactis IL1403 during heat, acid, and osmotic stress...
- Bioengineering of a Nisin A-producing Lactococcus lactis to create isogenic strains producing the natural variants Nisin F, Q and ZClare Piper
Department of Microbiology, University College Cork, College Road, Cork, Ireland
Microb Biotechnol 4:375-82. 2011..Nisins A, Z, F and Q are produced by Lactococcus lactis, while Nisins U and U2 are produced by Streptococcus sp...
- Sequencing of the tyrosine decarboxylase cluster of Lactococcus lactis IPLA 655 and the development of a PCR method for detecting tyrosine decarboxylating lactic acid bacteriaMaria Fernandez
Instituto de Productos Lacteos de Asturias, Carretera de Infiesto s n, 33300 Villaviciosa, Asturias, Spain
J Food Prot 67:2521-9. 2004..The Lactococcus lactis strain IPLA 655 was selected, and the genes encoding a putative tyrosyl tRNA synthetase, a tyrosine ..
- Lactococcus lactis NCC 2287 alleviates food allergic manifestations in sensitized mice by reducing IL-13 expression specifically in the ileumAdrian W Zuercher
Allergy Group, Department of Nutrition and Health, Nestle Research Center, Vers chez les Blanc, 1000 Lausanne 26, Switzerland
Clin Dev Immunol 2012:485750. 2012Utilizing a food allergy murine model, we have investigated the intrinsic antiallergic potential of the Lactococcus lactis NCC 2287 strain.
- Evaluation of oral immunization with recombinant avian influenza virus HA1 displayed on the Lactococcus lactis surface and combined with the mucosal adjuvant cholera toxin subunit BHan Lei
College of Life Science and Food Engineering, Nanchang University, Nanchang 330013, People s Republic of China
Clin Vaccine Immunol 18:1046-51. 2011..In this study, we constructed a recombinant (pgsA-HA1 gene fusion) Lactococcus lactis strain that expresses and displays the avian influenza virus HA1 antigens on its surface...
- Protection against tetanus toxin after intragastric administration of two recombinant lactic acid bacteria: impact of strain viability and in vivo persistenceCorinne Grangette
Département de Microbiologie des Ecosytèmes, Institut Pasteur de Lille, 59019 Lille Cedex, France
Vaccine 20:3304-9. 2002..this question, recombinant LAB strains, able to persist (Lactobacillus plantarum NCIMB8826/pMEC127) or not (Lactococcus lactis MG1363/pMEC46) in the gastro-intestinal tract of mice and producing equivalent amounts of the tetanus toxin ..
- DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanismsI Boucher
Department of Biochemistry and Microbiology, Faculte des sciences et de genie, Faculte de Medecine Dentaire, Universite Laval, Quebec, Canada
J Dairy Sci 84:1610-20. 2001The three Lactococcus lactis plasmids pSRQ700, pSRQ800, and pSRQ900 encode the previously described anti-phage resistance mechanisms LlaDCHI, AbiK, and AbiQ, respectively...
- Expression of bioactive porcine interferon-gamma by recombinant Lactococcus lactisPrithy Rupa
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Vet Microbiol 129:197-202. 2008b>Lactococcus lactis is an immunomodulator and candidate live mucosal delivery vehicle for vaccine antigens and for biologically active molecules, including immunoregulatory cytokines such as interferon-gamma (IFN-gamma)...
- Expression of mptC of Listeria monocytogenes induces sensitivity to class IIa bacteriocins in Lactococcus lactisManilduth Ramnath
Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa
Microbiology 150:2663-8. 2004..activity, the mptACD operon, encoding, was heterologously expressed in an insensitive species, namely Lactococcus lactis, using the NICE double plasmid system. Upon induction of the cloned operon, the recombinant Lc...
- RNA extraction from cheese for analysis of in situ gene expression of Lactococcus lactisV M Ulve
UMR1253, Science et Technologie du Lait et de l Oeuf, INRA, Rennes, France
J Appl Microbiol 105:1327-33. 2008..The isolation of high-quality RNA from cheese is a prerequisite for analysis of in situ gene expression of dairy micro-organisms...
- Multivitamin production in Lactococcus lactis using metabolic engineeringWilbert Sybesma
Department of Flavor, Nutrition and Natural Ingredients, Wageningen Centre for Food Sciences, NIZO Food Research, Kernhemseweg 2, P O Box 20, 6710 BA Ede, The Netherlands
Metab Eng 6:109-15. 2004The dairy starter bacterium Lactococcus lactis has the potential to synthesize both folate (vitamin B11) and riboflavin (vitamin B2)...
- Fate and efficacy of lacticin 3147-producing Lactococcus lactis in the mammalian gastrointestinal tractAlleson Dobson
Teagasc, Food Research Centre Moorepark, Fermoy, Co Cork, Ireland
FEMS Microbiol Ecol 76:602-14. 2011Gastrointestinal survival of the bacteriocin-producing strain, Lactococcus lactis DPC6520, was evaluated systematically in vitro and in vivo with a view to using this strain to deliver biologically active lacticin 3147, a broad-spectrum ..
- Modular structure of the receptor binding proteins of Lactococcus lactis phages. The RBP structure of the temperate phage TP901-1Silvia Spinelli
Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS and Université d Aix Marseille I, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France
J Biol Chem 281:14256-62. 2006b>Lactococcus lactis is a gram-positive bacterium widely used by the dairy industry. Several industrial L. lactis strains are sensitive to various distinct bacteriophages...
- Fibronectin-binding proteins of Staphylococcus aureus mediate activation of human platelets via fibrinogen and fibronectin bridges to integrin GPIIb/IIIa and IgG binding to the FcgammaRIIa receptorJ Ross Fitzgerald
Department of Microbiology, Moyne Institute of Preventive Medicine, University of Dublin, Trinity College, Dublin, Ireland
Mol Microbiol 59:212-30. 2006..aureus or Lactococcus lactis, indicating two distinct mechanisms of activation...
- Anatomy of a lactococcal phage tailStephen Mc Grath
Department of Microbiology, National University of Ireland, Cork, Ireland
J Bacteriol 188:3972-82. 2006..Furthermore, comparisons with lambda and other lactococcal phages allowed the specification of a number of genetic submodules likely to encode specific tail structures...
- Construction and characterization of a Lactococcus lactis strain deficient in intracellular ClpP and extracellular HtrA proteasesN G Cortes-Perez
Unité d Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
Microbiology 152:2611-8. 2006A Lactococcus lactis strain deficient in both its major proteases, intracellular (ClpP) and extracellular (HtrA), was constructed and characterized...
- Identification of the leucine-to-2-methylbutyric acid catabolic pathway of Lactococcus lactisBalasubramanian Ganesan
Western Dairy Center, Utah State University, Logan, UT 84322 4700, USA
Appl Environ Microbiol 72:4264-73. 2006..Phylogenetic analyses showed that homologues of these enzymes and their functional motifs were widespread across the domains of life...
- Improved stress tolerance of GroESL-overproducing Lactococcus lactis and probiotic Lactobacillus paracasei NFBC 338C Desmond
Teagasc, Biotechnology Centre, Moorepark, Fermoy, County Cork, Ireland
Appl Environ Microbiol 70:5929-36. 2004..These vectors were transferred into Lactococcus lactis(pGRO1) and L...
- Sequence analysis of the lactococcal plasmid pNP40: a mobile replicon for coping with environmental hazardsJonathan O'Driscoll
Department of Microbiology, University College Cork, Western Road, Cork, Ireland
J Bacteriol 188:6629-39. 2006The conjugative lactococcal plasmid pNP40, identified in Lactococcus lactis subsp...
- Immunogenicity and protective efficacy of orally administered recombinant Lactococcus lactis expressing surface-bound HIV EnvKe Qin Xin
Department of Bacteriology, Yokohama City University School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
Blood 102:223-8. 2003This study investigates whether genetically modified orally administered Lactococcus lactis (L lactis) could be used as an HIV vaccine. L lactis is immunogenic and extremely safe when delivered orally...
- Carbohydrate starvation causes a metabolically active but nonculturable state in Lactococcus lactisBalasubramanian Ganesan
Center for Integrated BioSystems, Utah State University, Logan, UT 84322 4700, USA
Appl Environ Microbiol 73:2498-512. 2007....
- Paracellular entry of interleukin-10 producing Lactococcus lactis in inflamed intestinal mucosa in miceAnouk Waeytens
Department of Pathology, Ghent University, Ghent, Belgium
Inflamm Bowel Dis 14:471-9. 2008Genetically modified Lactococcus lactis secreting interleukin-10 (IL-10) has been demonstrated to provide localized delivery of a therapeutic agent through active in situ synthesis in murine colitis...
- Lactococcus lactis as a live vector for mucosal delivery of therapeutic proteinsLuis G Bermúdez-Humarán
Equipe Interactions des bactéries commensales et probiotiques avec l hôte, Unité d Ecologie et Physiologie du Système Digestif, INRA 0910, Jouy en Josas, France
Hum Vaccin 5:264-7. 2009Food-grade lactic acid bacteria (LAB) have been safely consumed by humans for centuries in fermented foods. Lactococcus lactis, a LAB widely used in the dairy industry as a starter, can be genetically engineered to efficiently produce a ..
- Identification of tet(M) in two Lactococcus lactis strains isolated from a Spanish traditional starter-free cheese made of raw milk and conjugative transfer of tetracycline resistance to lactococci and enterococciAna Belén Flórez
Instituto de Productos Lácteos de Asturias CSIC, Carretera de Infiesto s n, 33300 Villaviciosa, Asturias, Spain
Int J Food Microbiol 121:189-94. 2008Specific PCR and sequencing showed that a tet(M) gene was present in two tetracycline-resistant Lactococcus lactis strains isolated from a raw milk, starter-free cheese...
- Complete genome sequence of Lactococcus lactis subsp. lactis KF147, a plant-associated lactic acid bacteriumRoland J Siezen
NIZO Food Research, Kluyver Centre for Genomics of Industrial Fermentation, P O Box 20, 6710 BA Ede, The Netherlands
J Bacteriol 192:2649-50. 2010b>Lactococcus lactis is a lactic acid bacterium used in the production of many fermented dairy products. We report the complete genome sequence of L. lactis subsp. lactis KF147, a nondairy strain isolated from mung bean sprouts...
- Amino acid accumulation limits the overexpression of proteins in Lactococcus lactisRavi K R Marreddy
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
PLoS ONE 5:e10317. 2010..In this study, we employed a proteomic approach to understand the difference in expression levels for various multidomain membrane proteins in L. lactis cells grown in complex and synthetic media...
- Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteinsDaniel M Linares
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Mol Biol 401:45-55. 2010..The generality and simplicity of our approach allow rapid improvements of protein production yields by directed evolution in a high-throughput way...
- Bovine rotavirus nonstructural protein 4 produced by Lactococcus lactis is antigenic and immunogenicV Enouf
, INRA, 78352 Jouy en Josas, France
Appl Environ Microbiol 67:1423-8. 2001..The capacity of the generally regarded as safe organism Lactococcus lactis to produce NSP4 either intra- or extracellularly was then investigated by using the nisin-controlled ..
- Multi-omics approach to study the growth efficiency and amino acid metabolism in Lactococcus lactis at various specific growth ratesPetri Jaan Lahtvee
Tallinn University of Technology, Department of Chemistry, Akadeemia tee 15, 12618 Tallinn, Estonia
Microb Cell Fact 10:12. 2011b>Lactococcus lactis is recognised as a safe (GRAS) microorganism and has hence gained interest in numerous biotechnological approaches...
- Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesisJ J Godon
Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
J Bacteriol 175:4383-90. 1993The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs...
- Proteome phenotyping of acid stress-resistant mutants of Lactococcus lactis MG1363Aurélie Budin-Verneuil
Laboratoire Microbiologie de l Environnement, EA956 USC INRA 2017, Universite de Caen, Caen, France
Proteomics 7:2038-46. 2007To cope with medium acidity, Lactococcus lactis has evolved a number of inducible mechanisms commonly referred as acid stress response...
- Gene inactivation in Lactococcus lactis: histidine biosynthesisC Delorme
Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
J Bacteriol 175:4391-9. 1993b>Lactococcus lactis strains from dairy and nondairy sources were tested for the ability to grow in the absence of histidine. Among 60 dairy strains tested, 56 required histidine, whereas only 1 of 11 nondairy strains had this requirement...
- Genetically engineered Lactococcus lactis secreting murine IL-10 modulates the functions of bone marrow-derived dendritic cells in the presence of LPSM Loos
Department for Molecular Biomedical Research, VIB, Ghent, Belgium
Scand J Immunol 69:130-9. 2009Oral delivery of IL-10 by genetically modified Lactococcus lactis (LL-pTmIL10) has been shown to efficiently reduce intestinal inflammation in mice with chronic colitis, but the mechanisms involved have not been elucidated...
- Lactococcus lactis, an efficient cell factory for recombinant protein production and secretionE Morello
Unité des Bactéries Lactiques et pathogènes Opportunistes UBLO, INRA, Jouy en Josas, France
J Mol Microbiol Biotechnol 14:48-58. 2008..The lactic acid bacterium Lactococcus lactis emerges as an attractive alternative to the Gram-positive model Bacillus subtilis...
- A. L. lactis-based vaccine for children with broad spectrum for enteric pathogensWendy L Picking; Fiscal Year: 2013..We will explore a novel platform technology consisting of nonliving, non-genetically modified, Lactococcus lactis particles, designated Gram-positive Enhancer Matrix (GEM) that will be engineered to display Type III ..
- Lipid Translocating Glycosyltransferases in Congenital Neurological DiseasePETER AB ORLEAN; Fiscal Year: 2010..Proteins will first be expressed in the Gram positive bacterium Lactococcus lactis, which targets polytopic membrane proteins to its single membrane...
- INTRON DYNAMICS IN BACTERIAMarlene Belfort; Fiscal Year: 2013..previous funding period, as follows: First, we will further explore our findings that dissemination of the Lactococcus lactis group II intron, Ll...
- Structural Dynamics of Drug/Proton Antiport by a Multidrug Efflux PumpPHILLIP RYAN STEED; Fiscal Year: 2013..Biochemical studies of two representatives of the DHA family, LmrP from Lactococcus lactis and MdfA from Escherichia coli, have shed light on the substrate specificity and the transport mechanism...
- Involvement of Proteins in Splicing Group I and II IntronsAlan M Lambowitz; Fiscal Year: 2013..For group II introns, we have used the Lactococcus lactis Ll...
- Non-peptidic HIV vaccineMiroslav Malkovsky; Fiscal Year: 2010..purpose we have developed highly immunogenic recombinant attenuated Listeria vaccine vectors and recombinant Lactococcus lactis vaccine particles...
- Analysis of Group I and II Introns in Mammalian CellsBruce Sullenger; Fiscal Year: 2005..More recently, we demonstrated that the Lactococcus lactis group II intron can reverse-splice and insert itself into DNA in transfected human cells...
- SLS: Molecular Basis and Role in Invasive GAS DiseaseVictor Nizet; Fiscal Year: 2006..for GAS SLS production and sufficient to confer SLS activity to the nonpathogenic heterologous species Lactococcus lactis. Sequence features and homologies strongly suggest SLS belongs to the bacteriocin class of toxins, with sagA ..
- C3-Binding and -Degrading Proteins in S. pneumoniaeMARGARET HOSTETTER; Fiscal Year: 2002..Truncation constructs expressed in Lactococcus lactis will be used to map the active site. A cppA- mutant in an encapsulated serotype 4 will be constructed...
- DISRUPTING VIRAL GENES WITH GROUP II INTRONSBruce Sullenger; Fiscal Year: 2004..Mobility of the Lactococcus lactis group II intron occurs through a two-step process...
- SUMO Fusions to Enhance Expression and Secretion of ProtiensTAUSEEF BUTT; Fiscal Year: 2007..coli. Recognizing the well-known protein secretion properties of Bacillus subtilis and Lactococcus lactis we also proposed to test the role of N-terminal of SUMO in secretion of protein in these gram-positive ..
- Mechanism of Group II Intron RetrotranspositionLORI CONLAN; Fiscal Year: 2005..overall goal of this project is to combine genetic, biochemical and structural studies to characterize the Lactococcus lactis group II intron-encoded protein (LtrA) and the pathways involved in intron mobility, particularly ..
- Cellular Osmoregulation:Sensors, Transducers& RegulatorsJanet Wood; Fiscal Year: 2003..is proposed since it supports prominent conference participants who study mechanisms that allow kidney cells to thrive despite the fluctuating osmolality, salinity and urea concentration associated with the renal concentrating mechanism ..
- Lactococcal vaccine for strep throatBruce Geller; Fiscal Year: 2004..The immediate, short term goals to be accomplished in phase I are: 1) Optimize surface expression in Lactococcus lactis of the conserved C-repeat region (CRR) of the M6 protein of Streptococcus pyogenes; 2) Optimize vaccine dose ..
- Flavoenzymes in Pyrimidine MetabolismBruce A Palfey; Fiscal Year: 2011..Our studies of the rates of the chemical reactions will identify important parts of the proteins, how they move during reactions, how they speed the synthesis of products, and how these reactions might be blocked. ..
- A BACTERIAL GROUP II INTRON INVOLVED IN CONJUGATIONGary Dunny; Fiscal Year: 2002..PI in the course of collaborative research with the coinvestigator on conjugative plasmid transfer of the Lactococcus lactis plasmid pRSO1...
- MOLECULAR SEQUENCE DATASamuel Karlin; Fiscal Year: 2001..by spacings between repeats, and by properties of repeat families (intergenic, coding, direct, inverted, mixed). ..
- Evolutionary Genomics of Anti-Malaria Genes in MosquitoBrian P Lazzaro; Fiscal Year: 2010..Evolutionary patterns in mosquito defense genes will be used to identify proteins that coevolve with malaria parasites. This work may suggest genetic targets that could be manipulated to limit malaria transmission. ..
- Exocyclic C-C Modifications of Aromatic PolyketidesJON THORSON; Fiscal Year: 2008..A particular focus of the final diversification strategies presented will be focused upon conjugation of these highly reactive antitumor antibiotics with known tumor-directing peptides. ..
- Virulence-conferring siderophore biosynthesis inhibitorsLUIS QUADRI; Fiscal Year: 2006..These antimicrobials will constitute a base for subsequent studies for the development of new drugs that, alone or in combination therapies, are anticipated to be useful in the treatment of MDR Yp and Mb infections. ..
- Cell Wall Components' Role in Enterococcal EndocarditisGary Dunny; Fiscal Year: 2006..faecalis virulence. 2) Determine the functional domains of Asc10 that confer the activities identified in Aim 1. 3) Examine the expression of bacterial and host genes during the course of infections. ..
- Gene Expression Levels Across Diverse GenomesSamuel Karlin; Fiscal Year: 2006..Staphylococcus aureus, Streptococcus pyogenes, and the nonpathogenic dairy fermentation bacterium Lactococcus lactis. The second group consists of all available high G+C a-proteobacteria...
- LETHAL OROESOPHAGEAL CANDIDIASIS: IMMUNOTHERAPYEdward Balish; Fiscal Year: 2004..Restoration and enhancement of immune function may be critical for the effectiveness of anti-Candida vaccines that are now under development. ..
- MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASESJON THORSON; Fiscal Year: 2002....
- GENETIC ANALYSIS OF GBS ADHERENCE TO FIBRONECTINGLEN TAMURA; Fiscal Year: 2002..These studies will allow for identification of the structural gene(s) for fibronectin adhesin(s). ..
- Analysis of Group B Streptococcal Fibrinogen CleavageDaniel Shelver; Fiscal Year: 2005..The availability of the purified protein will facilitate future biochemical structure-function studies on this important virulence factor of GBS. ..