gram negative bacteria

Summary

Summary: Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.

Top Publications

  1. pmc Accurate prediction of secreted substrates and identification of a conserved putative secretion signal for type III secretion systems
    Ram Samudrala
    Department of Microbiology, University of Washington, Seattle, Washington, United States of America
    PLoS Pathog 5:e1000375. 2009
  2. ncbi Quorum sensing in bacteria
    M B Miller
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Annu Rev Microbiol 55:165-99. 2001
  3. ncbi Bioinformatics, genomics and evolution of non-flagellar type-III secretion systems: a Darwinian perspective
    Mark J Pallen
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, Medical School, University of Birmingham, Birmingham, West Midlands B15 2TT, UK
    FEMS Microbiol Rev 29:201-29. 2005
  4. ncbi New beta-lactamases in gram-negative bacteria: diversity and impact on the selection of antimicrobial therapy
    K Bush
    The R W Johnson Pharmaceutical Research Institute, Raritan, NJ, 08869, USA
    Clin Infect Dis 32:1085-9. 2001
  5. ncbi Structure and function of bacterial outer membrane proteins: barrels in a nutshell
    R Koebnik
    Biozentrum Basel, Abteilung Mikrobiologie, Klingelbergstr 50, CH 4056 Basel, Switzerland
    Mol Microbiol 37:239-53. 2000
  6. pmc Antimicrobial-resistant pathogens in intensive care units in Canada: results of the Canadian National Intensive Care Unit (CAN-ICU) study, 2005-2006
    George G Zhanel
    Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    Antimicrob Agents Chemother 52:1430-7. 2008
  7. ncbi The bacterial type VI secretion machine: yet another player for protein transport across membranes
    Alain Filloux
    Imperial College London, Division of Cell and Molecular Biology, Centre for Molecular Microbiology and Infection, South Kensington Campus, Flowers Building, London SW7 2AZ, UK
    Microbiology 154:1570-83. 2008
  8. pmc Predicting subcellular localization of proteins for Gram-negative bacteria by support vector machines based on n-peptide compositions
    Chin Sheng Yu
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 30050, Taiwan
    Protein Sci 13:1402-6. 2004
  9. ncbi Type III secretion machines: bacterial devices for protein delivery into host cells
    J E Galan
    Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, CT 06536, USA
    Science 284:1322-8. 1999
  10. pmc Process of protein transport by the type III secretion system
    Partho Ghosh
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093 0314, USA
    Microbiol Mol Biol Rev 68:771-95. 2004

Detail Information

Publications319 found, 100 shown here

  1. pmc Accurate prediction of secreted substrates and identification of a conserved putative secretion signal for type III secretion systems
    Ram Samudrala
    Department of Microbiology, University of Washington, Seattle, Washington, United States of America
    PLoS Pathog 5:e1000375. 2009
    ..This approach provides a new way to identify secreted effectors in a broad range of pathogenic bacteria for further experimental characterization and provides insight into the nature of the type III secretion signal...
  2. ncbi Quorum sensing in bacteria
    M B Miller
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Annu Rev Microbiol 55:165-99. 2001
    ..Presumably, this process bestows upon bacteria some of the qualities of higher organisms. The evolution of quorum sensing systems in bacteria could, therefore, have been one of the early steps in the development of multicellularity...
  3. ncbi Bioinformatics, genomics and evolution of non-flagellar type-III secretion systems: a Darwinian perspective
    Mark J Pallen
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, Medical School, University of Birmingham, Birmingham, West Midlands B15 2TT, UK
    FEMS Microbiol Rev 29:201-29. 2005
    ..The problem of information overload is likely to be ameliorated by launch of a web site devoted to the comparative biology of type-III secretion ()...
  4. ncbi New beta-lactamases in gram-negative bacteria: diversity and impact on the selection of antimicrobial therapy
    K Bush
    The R W Johnson Pharmaceutical Research Institute, Raritan, NJ, 08869, USA
    Clin Infect Dis 32:1085-9. 2001
    ..Appreciation of these factors may help the physician make a more informed decision when choosing therapy to try to avoid selection of even more pathogenic strains...
  5. ncbi Structure and function of bacterial outer membrane proteins: barrels in a nutshell
    R Koebnik
    Biozentrum Basel, Abteilung Mikrobiologie, Klingelbergstr 50, CH 4056 Basel, Switzerland
    Mol Microbiol 37:239-53. 2000
    ..Our review is aimed at discussing their common principles and peculiarities as well as open questions associated with them...
  6. pmc Antimicrobial-resistant pathogens in intensive care units in Canada: results of the Canadian National Intensive Care Unit (CAN-ICU) study, 2005-2006
    George G Zhanel
    Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    Antimicrob Agents Chemother 52:1430-7. 2008
    ..aeruginosa, H. influenzae, Enterococcus spp., S. pneumoniae, and K. pneumoniae are the most common isolates recovered from clinical specimens in Canadian ICUs. A MDR phenotype is common for P. aeruginosa isolates in Canadian ICUs...
  7. ncbi The bacterial type VI secretion machine: yet another player for protein transport across membranes
    Alain Filloux
    Imperial College London, Division of Cell and Molecular Biology, Centre for Molecular Microbiology and Infection, South Kensington Campus, Flowers Building, London SW7 2AZ, UK
    Microbiology 154:1570-83. 2008
    ..The precise role and mode of action of the T6SS is still unknown. This review describes current knowledge about the T6SS and summarizes its hallmarks and its differences from other secretion systems...
  8. pmc Predicting subcellular localization of proteins for Gram-negative bacteria by support vector machines based on n-peptide compositions
    Chin Sheng Yu
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 30050, Taiwan
    Protein Sci 13:1402-6. 2004
    ..Because of its simplicity, this approach can be easily extended to other organisms and should be a useful tool for the high-throughput and large-scale analysis of proteomic and genomic data...
  9. ncbi Type III secretion machines: bacterial devices for protein delivery into host cells
    J E Galan
    Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, CT 06536, USA
    Science 284:1322-8. 1999
    ..Understanding the biology of these devices may allow the development of novel prevention and therapeutic approaches for several infectious diseases...
  10. pmc Process of protein transport by the type III secretion system
    Partho Ghosh
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093 0314, USA
    Microbiol Mol Biol Rev 68:771-95. 2004
    ..Numerous models explaining how effectors are transported into host cells have been proposed, but understanding of this process is incomplete and this topic remains an active area of inquiry...
  11. pmc Sequence-based prediction of type III secreted proteins
    Roland Arnold
    Department of Genome Oriented Bioinformatics, Technische Universitat Munchen, Wissenschaftszentrum Weihenstephan, Freising, Germany
    PLoS Pathog 5:e1000376. 2009
    ..Our findings will facilitate further studies on and improve our understanding of type III secretion and its role in pathogen-host interactions...
  12. ncbi Response of man to endotoxin
    G D Martich
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD
    Immunobiology 187:403-16. 1993
    ..Defining the pathways and responses initiated during acute human endotoxemia may allow a better understanding of host responses that are critical to the development of organ dysfunction and shock due to severe infections...
  13. ncbi Emerging issues in the management of infections caused by multidrug-resistant gram-negative bacteria
    Louis B Rice
    Louis Stokes Cleveland VA Medical Center and Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    Cleve Clin J Med 74:S12-20. 2007
    ....
  14. ncbi Decontamination of the digestive tract and oropharynx in ICU patients
    A M G A de Smet
    Division of Perioperative and Emergency Care, University Medical Center, Utrecht, The Netherlands
    N Engl J Med 360:20-31. 2009
    ....
  15. pmc Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat
    P A Bradford
    Wyeth Ayerst Research, Pearl River, New York 10965, USA
    Clin Microbiol Rev 14:933-51, table of contents. 2001
    ..They have also been found in other Enterobacteriaceae strains and Pseudomonas aeruginosa. Strains expressing these beta-lactamases will present a host of therapeutic challenges as we head into the 21st century...
  16. ncbi Biofilm formation as microbial development
    G O'TOOLE
    Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Annu Rev Microbiol 54:49-79. 2000
    ..The results reviewed in this article indicate that the formation of biofilms serves as a new model system for the study of microbial development...
  17. ncbi Quorum sensing, communication and cross-kingdom signalling in the bacterial world
    Paul Williams
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Microbiology 153:3923-38. 2007
    ....
  18. ncbi PSORTb v.2.0: expanded prediction of bacterial protein subcellular localization and insights gained from comparative proteome analysis
    J L Gardy
    Department of Molecular Biology and Biochemistry, Simon Fraser University Burnaby, BC, Canada V5A 1S6
    Bioinformatics 21:617-23. 2005
    ..The goals of the present work are as follows: increase PSORTb's coverage while maintaining the existing precision level, expand it to include Gram-positive bacteria and then carry out a comparative analysis of localization...
  19. ncbi The new beta-lactamases
    George A Jacoby
    Department of Infectious Diseases, Lahey Clinic, Burlington, Mass 01805, USA
    N Engl J Med 352:380-91. 2005
  20. ncbi Bacterial outer membrane vesicles and the host-pathogen interaction
    Meta J Kuehn
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genes Dev 19:2645-55. 2005
    ..Vesicle-mediated toxin delivery is a potent virulence mechanism exhibited by diverse Gram-negative pathogens. The biochemical and functional properties of pathogen-derived vesicles reveal their potential to critically impact disease...
  21. ncbi Type III secretion: a secretory pathway serving both motility and virulence (review)
    Laure Journet
    Biozentrum der Universitat Basel, Switzerland
    Mol Membr Biol 22:41-50. 2005
    ..Both appear to be assembled in the same fashion. They recognize their substrate by a loose N-terminal peptide signal and the help of individual chaperones of a new type...
  22. ncbi Protein secretion systems and adhesins: the molecular armory of Gram-negative pathogens
    Roman G Gerlach
    Institut fur Klinische Mikrobiologie, Immunologie und Hygiene, Universitatsklinikum Erlangen, Wasserturmstrasse 3 5, D 91054 Erlangen, Germany
    Int J Med Microbiol 297:401-15. 2007
    ..Here we give an overview on the recent understanding of the assembly of fimbrial and non-fimbrial adhesins and the role of type I, III and V secretion systems and specialized branches of the general secretion pathway in their biogenesis...
  23. ncbi The worldwide emergence of plasmid-mediated quinolone resistance
    Ari Robicsek
    Massachusetts General Hospital, Boston, MA 02114 2696, USA
    Lancet Infect Dis 6:629-40. 2006
    ....
  24. pmc Prediction of type III secretion signals in genomes of gram-negative bacteria
    Martin Löwer
    Johann Wolfgang Goethe University, Chair for Chem and Bioinformatics, Frankfurt, Germany
    PLoS ONE 4:e5917. 2009
    ..Although a special targeting signal at the N-terminal end of effector proteins has been proposed in literature its exact characteristics remain unknown...
  25. ncbi Type VI secretion: a beginner's guide
    Lewis Eh Bingle
    Centre for Systems Biology, University of Birmingham, Edgbaston, Birmingham, UK
    Curr Opin Microbiol 11:3-8. 2008
    ..In accordance with the general rule that the expression of protein secretion systems is tightly regulated, expression of type VI secretion is controlled at both transcriptional and post-transcriptional levels...
  26. ncbi The effect of protein-coated contact lenses on the adhesion and viability of gram negative bacteria
    Timothy J Williams
    Cooperative Research Centre for Eye Research and Technology, School of Optometry and Vision Science, Australia
    Curr Eye Res 27:227-35. 2003
    ..During contact lens wear, components of the tear film adsorb to the contact lens. This study aimed to investigate the effect of this conditioning film on the viability of bacteria...
  27. ncbi Polymyxin B for the treatment of multidrug-resistant pathogens: a critical review
    Alexandre Prehn Zavascki
    Infectious Diseases Service, Hospital São Lucas da Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
    J Antimicrob Chemother 60:1206-15. 2007
    ..Since polymyxins will be increasingly used for the treatment of infections caused by MDR bacteria, clinical pharmacokinetic, pharmacodynamic and toxicodynamic studies underpinning the optimal use of these drugs are urgently required...
  28. ncbi Development of a miniaturised microarray-based assay for the rapid identification of antimicrobial resistance genes in Gram-negative bacteria
    Miranda Batchelor
    Department of Food and Environmental Safety, Veterinary Laboratories Agency, Addlestone, Surrey, UK
    Int J Antimicrob Agents 31:440-51. 2008
    ....
  29. ncbi Distribution of multi-resistant Gram-negative versus Gram-positive bacteria in the hospital inanimate environment
    S W Lemmen
    Department of Infection Control, University Hospital Aachen, 52057 Aachen, Germany
    J Hosp Infect 56:191-7. 2004
    ..This benefits not only the patients, but also the hospital by removing some of the strain placed on already over-stretched resources...
  30. pmc PSORT-B: Improving protein subcellular localization prediction for Gram-negative bacteria
    Jennifer L Gardy
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada V5A 1S6
    Nucleic Acids Res 31:3613-7. 2003
    ..This dataset, the largest of its kind, is freely available, along with the PSORT-B source code (under GNU General Public License)...
  31. ncbi On the role of specific chaperones, the specific ATPase, and the proton motive force in type III secretion
    Gottfried Wilharm
    Max von Pettenkofer Institut, Lehrstuhl für Bakteriologie, Ludwig Maximilians Universitat Munchen, Pettenkoferstrasse 9a, D 80336 Munich, Germany
    Int J Med Microbiol 297:27-36. 2007
    ..Here, we focus on the role of specific T3SS chaperones, the specific ATPase, and the proton motive force in type III secretion...
  32. ncbi The rising influx of multidrug-resistant gram-negative bacilli into a tertiary care hospital
    Aurora E Pop-Vicas
    Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Clin Infect Dis 40:1792-8. 2005
    ....
  33. ncbi Extended-spectrum beta-lactamases: implications for the clinical microbiology laboratory, therapy, and infection control
    Enno Stürenburg
    Institut für Infektionsmedizin, Universitatsklinikum Hamburg Eppendorf, Hamburg, Germany
    J Infect 47:273-95. 2003
    ..Therapeutic options and potential strategies for dealing with this growing problem are also discussed in this article...
  34. pmc Type III protein secretion systems in bacterial pathogens of animals and plants
    C J Hueck
    Lehrstuhl fur Mikrobiologie, Biozentrum der Universität Würzburg, 97074 Wurzburg, Germany
    Microbiol Mol Biol Rev 62:379-433. 1998
    ..and the plant pathogens Pseudomonas syringae, Erwinia spp., Ralstonia solanacearum, Xanthomonas campestris, and Rhizobium spp...
  35. ncbi Structure and function of lipopolysaccharides
    Clett Erridge
    Medical Microbiology, University of Edinburgh Medical School, Edinburgh, Scotland, EH8 9AG, UK
    Microbes Infect 4:837-51. 2002
    ..Consideration is given in this review to the relationship between structure and function of these lipopolysaccharides...
  36. pmc The complete general secretory pathway in gram-negative bacteria
    A P Pugsley
    Unité de Génétique Moléculaire, Institut Pasteur, Paris, France
    Microbiol Rev 57:50-108. 1993
    ..This recently discovered branch pathway comprises at least 14 gene products. Other, simpler terminal branches of the GSP are also used by gram-negative bacteria to secrete a more limited range of extracellular proteins...
  37. ncbi The various and varying roles of specific chaperones in type III secretion systems
    Claude Parsot
    Unite de Pathogenie Microbienne Moleculaire, INSERM U389, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Curr Opin Microbiol 6:7-14. 2003
    ..Some chaperones are also directly involved in modulating transcription in response to secretion...
  38. ncbi Endotoxin-stimulated innate immunity: A contributing factor for asthma
    C E Reed
    Allergic Disease Research Laboratory, Mayo Clinic, Rochester, MN, USA
    J Allergy Clin Immunol 108:157-66. 2001
    ..The most effective management is mitigating exposure. The potential of drug treatments requires further clinical investigation...
  39. ncbi Bacteremia in children: etiologic agents, focal sites, and risk factors
    L F Nimri
    Department of Applied Biology, Jordan University of Science and Technology, Irbid
    J Trop Pediatr 47:356-60. 2001
    ..Resistance of the isolated pathogens to several commonly used antibiotics was observed. Empirical treatment with antibiotics is recommended only in life-threatening cases...
  40. ncbi The bactericidal/permeability-increasing protein (BPI), a potent element in host-defense against gram-negative bacteria and lipopolysaccharide
    P Elsbach
    Department of Medicine and Microbiology, New York University School of Medicine, New York
    Immunobiology 187:417-29. 1993
    ..Recombinant forms of both proteins protect animals against the lethal effects of administered LPS...
  41. ncbi Comparison of long-chain alcohols and other volatile compounds emitted from food-borne and related Gram positive and Gram negative bacteria
    Hesham Elgaali
    Department of Animal Sciences, University of Kentucky, Lexington, Kentucky 40546 0091, USA
    J Basic Microbiol 42:373-80. 2002
    ..Alcohols comprising 1-octanol, 1-decanol, and 1-dodecanol occurred as products from enteric Gram negative bacteria, which included Citrobacter, Enterobacter, Klebsiella, Salmonella, and Shigella...
  42. ncbi The type III secretion system tip complex and translocon
    C A Mueller
    Biozentrum der Universitat Basel, Basel, Switzerland
    Mol Microbiol 68:1085-95. 2008
    ..Unexpectedly, the third, hydrophilic translocator, forms a complex on the distal end of the injectisome needle, the tip complex, and serves as an assembly platform for the two hydrophobic translocators...
  43. pmc Control of gene expression by type III secretory activity
    Evan D Brutinel
    Department of Microbiology, University of Iowa, 540B Eckstein Medical Research Building, Iowa City, IA 52242 1101, USA
    Curr Opin Microbiol 11:128-33. 2008
    ..Although the major structural components of the flagellum and injectisome systems are highly conserved, recent studies reveal diversity in the mechanisms used by secretion substrates and chaperones to control gene expression...
  44. ncbi Subterfuge and manipulation: type III effector proteins of phytopathogenic bacteria
    Sarah R Grant
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Annu Rev Microbiol 60:425-49. 2006
    ..The eukaryotic host targets of only a few type III effector proteins are currently known. We also discuss possible mechanisms for diversification of the suite of type III effector proteins carried by a given bacterial strain...
  45. ncbi Antimicrobial susceptibility of multidrug-resistant Gram negative bacteria to fosfomycin
    M E Falagas
    Alfa Institute of Biomedical Sciences, 9 Neapoleos Street, 151 23 Marousi, Athens, Greece
    Eur J Clin Microbiol Infect Dis 27:439-43. 2008
    ..pneumoniae and, secondly, P. aeruginosa...
  46. ncbi Bacteremia and antimicrobial susceptibilities in HIV-infected patients at Siriraj Hospital
    Somporn Srifuengfung
    Department of Microbiology, Faculty of Medicine at Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Southeast Asian J Trop Med Public Health 36:347-51. 2005
    ..The changing spectrum of bacteria and antimicrobial susceptibility patterns in HIV-1 infected patients may provide a guideline for the selection of appropriate drugs for treatment...
  47. ncbi Overview of nosocomial infections caused by gram-negative bacilli
    Robert Gaynes
    Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Clin Infect Dis 41:848-54. 2005
    ..Gram-negative bacilli are commonly associated with hospital-acquired infections in ICUs. The proportion of Acinetobacter species associated with ICU pneumonia increased from 4% in 1986 to 7.0% in 2003...
  48. ncbi Contribution of Toll-like receptors to the innate immune response to Gram-negative and Gram-positive bacteria
    Greg Elson
    NovImmune SA, Geneva, Switzerland
    Blood 109:1574-83. 2007
    ..This study identifies dominant TLRs involved in responses to whole bacteria. It also validates the concept that host cell activation by bacterial pathogens can be therapeutically reduced by anti-TLR4, -TLR2, and -CD14 mAbs...
  49. ncbi [Beta-lactamases of Gram negative bacteria: never-ending clockwork!]
    A Philippon
    Service de Bacteriologie, CHU Cochin, AP HP, Paris
    Ann Biol Clin (Paris) 64:37-51. 2006
    ....
  50. ncbi DNA-DNA hybridization values and their relationship to whole-genome sequence similarities
    Johan Goris
    Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA
    Int J Syst Evol Microbiol 57:81-91. 2007
    ..It is concluded that ANI can accurately replace DDH values for strains for which genome sequences are available...
  51. ncbi The lactoferrin receptor complex in Gram negative bacteria
    Amanda J Beddek
    Department of Microbiology and Infectious Diseases, University of Calgary, Rm G503A Health Sciences Center, 3330 Hospital Drive N W, Calgary, AB, Canada T2N 4N1
    Biometals 23:377-86. 2010
    ..The lactoferrin receptors in these species appear to be essential for survival and thus may serve as potential vaccine targets...
  52. pmc PapD-like chaperones provide the missing information for folding of pilin proteins
    M M Barnhart
    Departments of Molecular Microbiology and Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 97:7709-14. 2000
    ..This mechanism of small periplasmic chaperone function described here deviates from classical hsp60 chaperone-assisted folding...
  53. ncbi Fiber assembly by the chaperone-usher pathway
    Frederic G Sauer
    Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Ave, New Haven, CT 06536, USA
    Biochim Biophys Acta 1694:259-67. 2004
    ..Chaperone dissociation releases the folding energy that drives subunit incorporation into the fiber, which grows through a pore formed by the outer-membrane usher...
  54. ncbi Type 1 protein secretion in bacteria, the ABC-transporter dependent pathway (review)
    I Barry Holland
    Institut de Genetique et Microbiologie, CNRS UMR 8621, Universite Paris Sud, Orsay Cedex, France
    Mol Membr Biol 22:29-39. 2005
    ....
  55. ncbi Atomic force microscopy study of the antimicrobial action of Sushi peptides on Gram negative bacteria
    A Li
    Department of Mechanical Engineering, National University of Singapore, Singapore
    Biochim Biophys Acta 1768:411-8. 2007
    ..The AFM results support our hypothesis that the S3 peptide perturbs the GNB membrane via the "carpet-model" and thus, provide important insights into their antimicrobial mechanisms...
  56. pmc Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs
    C Goffin
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B 4000 Sart Tilman Liège, Belgium
    Microbiol Mol Biol Rev 62:1079-93. 1998
    ..Studies of the reaction pathways by using the methods of quantum chemistry suggest that resistance to penicillin is a road of no return...
  57. ncbi Measuring the impact of multidrug resistance in nosocomial infection
    Stijn Blot
    Intensive Care Department, Ghent University Hospital, Ghent, Belgium
    Curr Opin Infect Dis 20:391-6. 2007
    ..Methodological techniques dealing with the problem of confounding are discussed and current findings in how multidrug resistance affects outcome in patients with nosocomial infection are highlighted...
  58. ncbi Lipid A diversity and the innate host response to bacterial infection
    R P Darveau
    Department of Periodontics, Box 357444, School of Dentistry, Universityof Washington, Seattle, WA 98195 7444, USA
    Curr Opin Microbiol 1:36-42. 1998
    ..Recent data indicate that bacteria can regulate this molecule in response to different host microenvironments. Host factors that induce lipid A modifications and the resultant changes in host response remain to be determined...
  59. ncbi Chaperones of the type III secretion pathway: jacks of all trades
    Anne Laure Page
    Unite de Pathogenie Microbienne Moleculaire, INSERM U389, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, France
    Mol Microbiol 46:1-11. 2002
    ..The flagellar export apparatus is closely related to the TTS apparatus and some proteins of the flagellar export system have also been proposed to be chaperones that prevent premature interactions between the flagellum subunits...
  60. pmc Bloodstream infections caused by antibiotic-resistant gram-negative bacilli: risk factors for mortality and impact of inappropriate initial antimicrobial therapy on outcome
    Cheol In Kang
    Department of Internal Medicine, Seoul National University College of Medicine, 28 Yongon dong Chongno gu, Seoul 110 744, Republic of Korea
    Antimicrob Agents Chemother 49:760-6. 2005
    ..72; P = 0.030). Our data suggest that inappropriate initial antimicrobial therapy is associated with adverse outcome in antibiotic-resistant gram-negative bacteremia, particularly in patients with a high-risk source of bacteremia...
  61. pmc The versatile bacterial type IV secretion systems
    Eric Cascales
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA
    Nat Rev Microbiol 1:137-49. 2003
    ....
  62. ncbi Emerging bacterial resistance patterns in febrile neutropenic patients: experience at a tertiary care hospital in Pakistan
    M A Khan
    Department of Medicine, The Aga Khan University Hospital, Karachi
    J Pak Med Assoc 54:357-60. 2004
    ..To look at the clinical presentations, spectrum and site of isolation of the organisms, sensitivity patterns of the organisms and the antibiotic prescribing practices for the treatment of febrile neutropenic patients at our hospital...
  63. ncbi Controlling infection by tuning in and turning down the volume of bacterial small-talk
    Miguel Camara
    School of Pharmaceutical Sciences, University of Nottingham, Nottingham, UK
    Lancet Infect Dis 2:667-76. 2002
    ..As a consequence, this event, now termed quorum sensing, represents a novel therapeutic target offering the opportunity to attenuate virulence, and thus control infection, by blocking cell-to-cell communication...
  64. pmc Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting gram-negative bacilli from patients with cystic fibrosis
    Xuan Qin
    Department of Laboratory Medicine, Therapeutics Development Network Resource Center for Microbiology, Children s Hospital and Regional Medical Center, University of Washington, Seattle, Washington, USA
    J Clin Microbiol 41:4312-7. 2003
    ..Given the diversity of these CF isolates, real-time PCR with a combination of two target sequences appears to be the optimum choice for identification of atypical P. aeruginosa and for non-P. aeruginosa gram-negative isolates...
  65. pmc Use of pyrosequencing of 16S rRNA fragments to differentiate between bacteria responsible for neonatal sepsis
    Jeanne A Jordan
    Department of Pathology, University of Pittsburgh, Magee Women s Research Institute, 204 Craft Avenue, Pittsburgh, PA 15213, USA
    J Mol Diagn 7:105-10. 2005
    ..Results of DNA sequencing and culture identification were compared. In summary, we were successful at using PCR and pyrosequencing together to accurately differentiate between highly diverse bacterial groups...
  66. ncbi Resistance in nonfermenting gram-negative bacteria: multidrug resistance to the maximum
    John E McGowan
    Department of Epidemiology, Rollins School of Public Health, Emory University, Atlanta, Georgia 30322, USA
    Am J Med 119:S29-36; discussion S62-70. 2006
    ..Improved antibiotic stewardship and infection-control measures will be needed to prevent or slow the emergence and spread of multidrug-resistant, nonfermenting gram-negative bacilli in the healthcare setting...
  67. ncbi Polymyxin B sulfate and colistin: old antibiotics for emerging multiresistant gram-negative bacteria
    M E Evans
    Department of Internal Medicine, College of Medicine, University of Kentucky, Lexington 40536, USA
    Ann Pharmacother 33:960-7. 1999
    ..Gram-negative bacteria that are resistant to the aminoglycosides, beta-lactams, and fluoroquinolones are becoming more common. These bacteria are often susceptible to the polymyxins...
  68. pmc Transposon Tn21, flagship of the floating genome
    C A Liebert
    Department of Microbiology, The University of Georgia, Athens, Georgia 30602 2605, USA
    Microbiol Mol Biol Rev 63:507-22. 1999
    ..The compendium offered here will provide a reference point for ongoing observations of related elements in multiply resistant strains emerging worldwide...
  69. ncbi Nosocomial bacterial infections in Intensive Care Units. I: Organisms and mechanisms of antibiotic resistance
    S M Lim
    Department of Anaesthesia and Intensive Care, St Mary s Hospital, Praed Street, London W2 1NY, UK
    Anaesthesia 60:887-902. 2005
    ..This review discusses the reasons for these phenomena and describes the mechanisms underlying antibiotic resistance and the common intensive care unit-acquired organisms...
  70. ncbi Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997-2002)
    Douglas J Biedenbach
    The JONES Group JMI Laboratories, North Liberty, IA, USA
    Diagn Microbiol Infect Dis 50:59-69. 2004
    ..Surveillance of BSI pathogens is needed to determine trends of resistance and provide useful information regarding patient risk factors and geographic differences...
  71. pmc New antibiotic molecules: bypassing the membrane barrier of gram negative bacteria increases the activity of peptide deformylase inhibitors
    Laurent Mamelli
    UMR MD1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultes de Medecine et de Pharmacie, IFR 88, Universite de la Mediterranee, Marseille, France
    PLoS ONE 4:e6443. 2009
    ..The bacterial susceptibility to such a cytoplasmic target primarily depends on the capacity of the compound to reach and accumulate in the cytosol...
  72. pmc Global spread of multiple aminoglycoside resistance genes
    Kunikazu Yamane
    National Institute of Infectious Diseases, Tokyo, Japan
    Emerg Infect Dis 11:951-3. 2005
    ..ArmA, which had been identified exclusively in Europe, was also found in several gram-negative pathogenic bacilli isolated in Japan, suggesting global dissemination of hazardous multiple aminoglycoside resistance genes...
  73. ncbi Nosocomial bacteremia caused by antibiotic-resistant gram-negative bacteria in critically ill patients: clinical outcome and length of hospitalization
    Stijn Blot
    Department of Intensive Care, Ghent University Hospital, B 9000 Ghent, Belgium
    Clin Infect Dis 34:1600-6. 2002
    ..032) were independently associated with in-hospital mortality (P<.05). Antibiotic resistance in nosocomial bacteremia caused by gram-negative bacteria does not adversely affect the outcome for critically ill patients...
  74. pmc Polyamine effects on antibiotic susceptibility in bacteria
    Dong Hyeon Kwon
    Department of Biology, Georgia State University, 24 Peachtree Center Avenue, Atlanta, GA 30303, USA
    Antimicrob Agents Chemother 51:2070-7. 2007
    ..In summary, this interesting finding of the effect of spermine on antibiotic susceptibility provides the basis for a new potential approach against drug-resistant pathogens by use of existing beta-lactam antibiotics...
  75. ncbi Type III protein secretion mechanism in mammalian and plant pathogens
    Sheng Yang He
    Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
    Biochim Biophys Acta 1694:181-206. 2004
    ..Here, we summarize the most salient aspects of the TTSS, with special emphasis on recent findings...
  76. ncbi Transforming growth factor-beta 1 inhibits non-pathogenic Gram negative bacteria-induced NF-kappa B recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation
    Dirk Haller
    Department of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:23851-60. 2003
    ..We concluded that the TGF-beta1/Smad signaling pathway helps maintain normal intestinal homeostasis to commensal luminal enteric bacteria by regulating NF-kappaB signaling in IEC through altered histone acetylation...
  77. ncbi The bacterial injection kit: type III secretion systems
    Luís J Mota
    Biozentrum der Universitat Basel, Switzerland
    Ann Med 37:234-49. 2005
    ....
  78. ncbi Essential oils: their antibacterial properties and potential applications in foods--a review
    Sara Burt
    Department of Public Health and Food Safety, Faculty of Veterinary Medicine, University of Utrecht, P O Box 80175, 3508 TD Utrecht, The Netherlands
    Int J Food Microbiol 94:223-53. 2004
    ..Some EO components are legally registered flavourings in the EU and the USA. Undesirable organoleptic effects can be limited by careful selection of EOs according to the type of food...
  79. ncbi Meropenem activity against European isolates: report on the MYSTIC (Meropenem Yearly Susceptibility Test Information Collection) 2006 results
    Philip J Turner
    Infection Discovery Department, AstraZenca, Alderley House, Alderley Park, Macclesfield, SK10 4TG Cheshire, UK
    Diagn Microbiol Infect Dis 60:185-92. 2008
    ..The continued need for surveillance studies such as MYSTIC in order that correct clinical decisions concerning antibiotic selection are made is once again demonstrated...
  80. ncbi Contemporary activity of meropenem and comparator broad-spectrum agents: MYSTIC program report from the United States component (2005)
    Paul R Rhomberg
    JMI Laboratories, North Liberty, IA 52317, USA
    Diagn Microbiol Infect Dis 57:207-15. 2007
    ....
  81. ncbi Contemporary causes of skin and soft tissue infections in North America, Latin America, and Europe: report from the SENTRY Antimicrobial Surveillance Program (1998-2004)
    Gary J Moet
    JMI Laboratories, North Liberty, IA 52317, USA
    Diagn Microbiol Infect Dis 57:7-13. 2007
    ..48.0%) when compared with other regions. Continued surveillance of pathogen prevalence and antimicrobial resistance patterns should provide information that is important to improve empiric care particularly in the hospital environment...
  82. ncbi Assessment of pathogen occurrences and resistance profiles among infected patients in the intensive care unit: report from the SENTRY Antimicrobial Surveillance Program (North America, 2001)
    Jennifer M Streit
    The JONES Group JMI Laboratories Inc, 345 Beaver Kreek Centre, Suite A, North Liberty, IW 52317, USA
    Int J Antimicrob Agents 24:111-8. 2004
    ..Cefepime and the carbapenems (imipenem or meropenem) for Gram-negative isolates and linezolid for Gram-positive isolates, provided the broadest spectrum of in vitro activity against contemporary ICU pathogens in North America...
  83. ncbi Moxifloxacin prophylaxis in neutropenic patients
    H von Baum
    Department of Medical Microbiology and Hygiene, University Hospital, Ulm, Germany
    J Antimicrob Chemother 58:891-4. 2006
    ..Among the fluoroquinolones moxifloxacin currently provides one of the broadest spectra of antibacterial activity and may be suitable for prophylaxis during neutropenia...
  84. ncbi Trends in antimicrobial susceptibility in UK centres: the MYSTIC Programme (1997-2002)
    Robert G Masterton
    Crosshouse Hospital, Kilmarnock, Aryshire, UK
    Int J Antimicrob Agents 27:69-72. 2006
    ..Although all antibiotics tested retained acceptable activity, the carbapenems remained the most active antimicrobial agents against Gram-negative bacteria, including ESBL- and AmpC-producing isolates...
  85. ncbi Cell wall substrate specificity of six different lysozymes and lysozyme inhibitory activity of bacterial extracts
    Dorothy Nakimbugwe
    Laboratory of Food Microbiology, Katholieke Universiteit Leuven, Leuven, Belgium
    FEMS Microbiol Lett 259:41-6. 2006
    ....
  86. pmc Atypical infections in tsunami survivors
    Christian Garzoni
    University Hospitals of Geneva, Geneva, Switzerland
    Emerg Infect Dis 11:1591-3. 2005
    ..Treating these infections is challenging from a clinical and microbiologic point of view...
  87. ncbi Bacteremia in patients with hematological malignancies
    Fu Der Wang
    Section of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan
    Chemotherapy 51:147-53. 2005
    ..The aim of this study was to investigate isolates of bacteremia and their antibiotic susceptibility in patients with hematological malignancies...
  88. ncbi [Analysis of microorganisms isolated from febrile neutropenic children with neoplastic disease]
    Ilona Wojak
    Laboratorium Mikrobiologiczne Wojewódzkiego, Szpitala Dzieciecego w Bydgoszczy
    Med Dosw Mikrobiol 56:411-9. 2004
    ..faecium, S. haemolyticus. All strains Staphylococcus spp. were sensitive to vancomycin. There have not been found Enterococcus spp. resistant to glycopeptides. Most active against Gram-positive rods were carbapenems and aminoglycosides...
  89. ncbi Antimicrobial activity of some medicinal plants of the island Soqotra
    Ramzi A A Mothana
    Department of Pharmacognosy, Faculty of Pharmacy, Sana a University, PO Box 33039, Sana a, Yemen
    J Ethnopharmacol 96:177-81. 2005
    ..Only the methanolic extract of Buxus hildebrandtii displayed significant antifungal activity...
  90. ncbi Interactions between folding factors and bacterial outer membrane proteins
    Jesper E Mogensen
    Department of Life Sciences, Aalborg University, Sohngaardsholmsvej 49, DK 9000 Aalborg, Denmark
    Mol Microbiol 57:326-46. 2005
    ..Important advances have clearly been made in the field, but much work remains to be done, particularly in terms of describing the biophysical basis for the chaperone-OMP interactions which so intricately regulate OMP biogenesis...
  91. pmc Protein subcellular localization prediction for Gram-negative bacteria using amino acid subalphabets and a combination of multiple support vector machines
    Jiren Wang
    Bioinformatics Institute, Matrix, Singapore 138671
    BMC Bioinformatics 6:174. 2005
    ..However, these methods had relatively low accuracies for the localization of extracellular proteins. This paper studies ways to improve the accuracy for predicting extracellular localization in Gram-negative bacteria...
  92. ncbi Type III secretion: the bacteria-eukaryotic cell express
    Luis Jaime Mota
    Biozentrum der Universitat Basel, Biozentrum, Klingelbergstrasse, 50 70 CH4051 Basel, Switzerland
    FEMS Microbiol Lett 252:1-10. 2005
    ..Here, we review these different aspects focusing in open questions that promise exciting findings in the near future...
  93. ncbi When to include polymyxins in the empirical antibiotic regimen in critically ill patients with fever? A decision analysis approach
    Matthew E Falagas
    Alfa Institute of Biomedical Sciences, Athens, Greece
    Shock 27:605-9. 2007
    ..baumannii, P. aeruginosa, or K. pneumoniae) is polymyxin-only-susceptible is close to that (50%) used in our model (based on the individual hospital data)...
  94. ncbi Prevalence of extended-spectrum beta-lactamase-producing Gram-negative bacteria in septicaemic neonates in a tertiary care hospital
    Amita Jain
    Departments of Microbiology, King George s Medical College, Lucknow, UP 226 003, India
    J Med Microbiol 52:421-5. 2003
    ..It is concluded that indiscriminate use of third-generation cephalosporins may be responsible for the selection of ESBL-producing multiresistant strains in the neonatal intensive-care unit (NICU)...
  95. ncbi Quorum sensing inhibition: targeting chemical communication in gram-negative bacteria
    Tobias Persson
    Department of Natural Sciences, Bioorganic Chemistry Section, The Royal Danish Veterinary and Agricultural University, DK 1871 Frederiksberg C, Denmark
    Curr Med Chem 12:3103-15. 2005
    ..Biological activity is rationalized on the basis of structure-activity relationships and structural insight into the target protein...
  96. pmc Common TNF-alpha, IL-1 beta, PAI-1, uPA, CD14 and TLR4 polymorphisms are not associated with disease severity or outcome from Gram negative sepsis
    Kristine Marie Jessen
    Clinical Research Unit, Hvidovre University Hospital, Copenhagen, Denmark
    BMC Infect Dis 7:108. 2007
    ..Only few studies have combined the analysis of multiple SNPs in the same population...
  97. ncbi Evaluation of the in vitro activity of six broad-spectrum beta-lactam antimicrobial agents tested against recent clinical isolates from India: a survey of ten medical center laboratories
    Dilip Mathai
    Christian Medical College Hospital, Vellore, India
    Diagn Microbiol Infect Dis 44:367-77. 2002
    ..Collaborative studies, such as those presented here, can accurately detect changes in resistance patterns, and their continued use may help limit the further development and spread of bacterial resistances in India...
  98. ncbi Comparative activity of meropenem in US medical centers (2007): initiating the 2nd decade of MYSTIC program surveillance
    Ronald N Jones
    JMI Laboratories, North Liberty, IA 52317, USA
    Diagn Microbiol Infect Dis 61:203-13. 2008
    ....
  99. pmc Protection from lethal gram-negative bacterial sepsis by targeting Toll-like receptor 4
    Thierry Roger
    Infectious Diseases Service, Department of Medicine, Centre Hospitalier Universitaire Vaudois and University of Lausanne, CH 1011 Lausanne, Switzerland
    Proc Natl Acad Sci U S A 106:2348-52. 2009
    ..coli sepsis when administered in a prophylactic and therapeutic manner up to 13 h after the onset of bacterial sepsis. These experimental data provide strong support for the concept of TLR4-targeted therapy for gram-negative sepsis...
  100. ncbi Real-Time PCR quantification of PAH-ring hydroxylating dioxygenase (PAH-RHDalpha) genes from Gram positive and Gram negative bacteria in soil and sediment samples
    Aurélie Cébron
    Laboratoire des Interactions Microorganismes Minéraux Matière Organique dans les Sols UMR7137, Nancy Universite, CNRS, Faculte des Sciences, B P 239, 54506 Vandoeuvre les Nancy Cedex, France
    J Microbiol Methods 73:148-59. 2008
    ..This ratio highlighted a positive correlation between the PAH-bacterial biodegradation potential and the PAH-contamination level in the environmental samples studied...
  101. ncbi Natural history of colonization with vancomycin-resistant enterococci, methicillin-resistant Staphylococcus aureus, and resistant gram-negative bacilli among long-term-care facility residents
    Glenn A Pacio
    Division of Infectious Diseases, New York Medical College, Valhalla 10595, USA
    Infect Control Hosp Epidemiol 24:246-50. 2003
    ..To determine the natural history of colonization with vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus (MRSA), and resistant gram-negative bacilli among long-term-care facility (LTCF) residents...

Research Grants72

  1. Anti-Inflammatory Glycosaminoglycan Ethers for Treatment of Periodontitis
    JUSTIN RODNEY SAVAGE; Fiscal Year: 2011
    ..We propose to develop anionic, partially lipophilic hyaluronic acid derivatives as a simple mechanistically-based treatment for this chronic extraordinarily common dental disorder. ..
  2. Periodontal Biomaterials with BITE (Biofilm Immunity via T-cell Enhancement)
    James D Bryers; Fiscal Year: 2012
    ..Our goal is to develop tissue regenerative biomaterials that will also provide a short-term defense and life long immune response to specific bacterial infections ..
  3. Targeting superbugs: discovery and development of new broad-spectrum lipopeptides
    Jian Li; Fiscal Year: 2013
    ..aeruginosa, A. baumannii and K. pneumoniae. Overall, this project targets the urgent global unmet medical need and responds in a timely manner to the recent global call for discovery of new antibiotics: The 10 x '20 Initiative. ..
  4. Endotoxin, the airway transcriptome, and obstructive lung disease
    PEGGY SUE LAI; Fiscal Year: 2013
    ..Endotoxin is derived from gram negative bacteria, and has been associated with both asthma and COPD...
  5. Optimization of Neoglycoside Antibiotics for Nosocomial Pathogens and Select Agen
    Arnold Louie; Fiscal Year: 2013
    ....
  6. Mechanisms of innate immune response modulation by mechanical ventilation
    William A Altemeier; Fiscal Year: 2012
    ..This project will help identify mechanisms by which mechanical ventilation support causes lung injury;thereby, providing guidance for the development of future interventions to decrease the incidence and severity of acute lung injury. ..
  7. 5500 QTRAP
    John W Turk; Fiscal Year: 2010
    ..Such information will provide insight into developing new ways to diagnose and treat human diseases and thereby improve human health. ..
  8. ENZYMOLOGY OF ANTIBIOTIC RESISTANCE
    Richard Armstrong; Fiscal Year: 2009
    ..These investigations will establish the bases for the design of new drugs to counter resistance to fosfomycin that is encoded in the genomes of select human pathogens. ..
  9. X-ray Studies of Sugar-Modifying Enzymes
    Hazel Holden; Fiscal Year: 2013
    ....
  10. Group B. Streptococci and Toll-like Receptors
    Douglas T Golenbock; Fiscal Year: 2012
    ..The goal of this grant is to identify the streptococcal molecules and the receptors they activate in order to diminish the severity of Group B streptococcal infection in humans. ..
  11. Lactoferrin for Immunomodulation of Systemic Inflammatory Response Syndrome
    Marian L Kruzel; Fiscal Year: 2011
    ..The goal of this proposal is to examine the utility of a novel human recombinant Lactoferrin to combat SIRS, and limit progression of SIRS. ..
  12. Regulation of Bacteroides fragilis multidrug efflux pumps
    Hannah Wexler; Fiscal Year: 2013
    ..Bacteroides fragilis is an important member of the gut microbiota that has critical roles in human health and disease. We have virtually no information about these pumps or their regulation in this important anaerobic pathogen. ..
  13. Bacterial Immunosuppression: Host Target Identification Through Chemical Probes
    Kim D Janda; Fiscal Year: 2013
    ..From these studies, we hope to validate C12 target proteins and begin to provide functional links between these proteins and the biological activities of C12. ..
  14. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    Pei Zhou; Fiscal Year: 2012
    ....
  15. GENETICS AND VIRULENCE OF PNEUMOCOCCAL CAPSULAR TYPES
    JANET L YOTHER; Fiscal Year: 2012
    ..By understanding the mechanisms involved in this process, we may be better able to develop means for treating and preventing these infections. ..
  16. The Roles of Conserved outer-membrane proteins in SFG rickettsia pathogenesis
    Juan J Martinez; Fiscal Year: 2013
    ....
  17. Molecular mechanism of apolipoprotein binding to lipopolysaccharides
    PAUL MICHIEL WEERS; Fiscal Year: 2013
    ..The proposed research aims to understand the molecular basis of the protective role of apolipoproteins, thereby providing a foundation for improving the treatment of bacterial sepsis. ..
  18. Probiotics and HIF signaling in Alcoholic Liver Disease
    Wenke Feng; Fiscal Year: 2013
    ..This study will have a major impact on the development of new therapeutic targets for the prevention and treatment of ALD. ..
  19. Molecular mechanisms of pathogen recognition by the Nod-like receptor NLRC4
    Fayyaz S Sutterwala; Fiscal Year: 2012
    ..iii) Determining if NLRC4 recognizes host plasma membrane components following their disruption by bacterial T3SS. ..
  20. Gonococcal Mechanisms to Evade Host Defenses
    WILLIAM MAURICE SHAFER; Fiscal Year: 2013
    ..With more female veterans using the Medical Service as their primary mode of care, it is likely that VA Medical Centers will experience the health care costs associated with invasive gonococcal infections. ..
  21. Challenges in beta-Lactamase Mediated Resistance
    Robert A Bonomo; Fiscal Year: 2013
    ..We chose these [unreadable]-lactamases as they are among the most important class A and class C enzymes in Gram-negative bacteria. ..
  22. Novel gene-silencing therapeutics for multidrug-resistant gram-negative pathogens
    DAVID ELIHU GREENBERG; Fiscal Year: 2013
    ..This project will identify lead target PPMOs in E. coli and A. baumannii that can be moved forward to pre-clinical and clinical studies. ..
  23. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2012
    ..abstract_text> ..
  24. HTS assay development targeting Yersinia pestis topoisomerase I
    Yuk Ching Tse-Dinh; Fiscal Year: 2011
    ..Y. pestis topoisomerase I shares extensive sequence homology with topoisomerase I from E. coli and other gram negative bacteria. It is expected that the molecules identified for Y...
  25. Epigenetic regulation of viral infection and replication by periodontal bacteria
    Fengchun Ye; Fiscal Year: 2013
    ..The results should provide a scientific basis for developing novel strategies to prevent and treat recurrent HIV and herpesviral infections in HIV/AIDS patients simultaneously. ..
  26. Regulation and Function of Adiponectin Oligomerization
    Feng Liu; Fiscal Year: 2012
    ....
  27. Plasmid-Mediated Quinolone Resistance
    George A Jacoby; Fiscal Year: 2013
    ..Under Specific Aim 3, we propose to explore Qnr/gyrase interaction as revealed by isothermal titration calorimetry or surface plasmon resonance and by x- ray crystallography. ..
  28. Milk osteopontin, a nutritional therapeutic intervention for alcoholic hepatitis
    Natalia Nieto; Fiscal Year: 2013
    ....
  29. REGULATION OF LIPID METABOLISM IN BACTERIA
    Charles O Rock; Fiscal Year: 2013
    ..This research will provide a definitive answer regarding the suitability of membrane lipid synthesis in Gram-positive pathogens as a target for novel antibacterial drug discovery development. ..
  30. Allosteric regulation of the sensory protease DegS
    RANDALL VERNON MAULDIN; Fiscal Year: 2011
    ..The research described in this proposal will broaden and deepen our understanding of the allosteric mechanisms used by the DegS sensory protease to facilitate bacterial survival under inhospitable conditions. ..
  31. ETHANOL REGULATION OF KUPFFER CELL SIGNAL TRANSDUCTION
    Laura E Nagy; Fiscal Year: 2013
    ....
  32. Spin labeling of MsbA
    Candice S Klug; Fiscal Year: 2012
    ....
  33. Identifying novel anti-infectives by high through-put screening in whole animals
    Frederick M Ausubel; Fiscal Year: 2013
    ..Rather than simply preventing bacteria from growing, these new sophisticated drugs will prevent disease by interfering with a microbe's ability to interact with the human body. ..
  34. Cholecystokinin - Role in postprandial gastric motility
    Helen E Raybould; Fiscal Year: 2013
    ....
  35. BIOSYNTHESIS AND FUNCTION OF LIPOPOLYSACCHARIDES
    Pei Zhou; Fiscal Year: 2013
    ....
  36. Innate immune signal transduction specificity in inflammatory disease
    Derek W Abbott; Fiscal Year: 2013
    ..This work aims to help determine the causes of inflammatory disease and aims to identify novel targets for pharmaceutical intervention in these debilitating disorders. ..
  37. Immunogenicity of the Type IV Secretin System
    WENDY CATHERINE BROWN; Fiscal Year: 2012
    ..The results of this project will be generally applicable vaccine development for many human bacterial diseases. ..
  38. Synthetic Oligosaccharides for Probing Infection and Innate Immunity
    Geert Jan Boons; Fiscal Year: 2013
    ..The new methodology will be employed to probe the ligand requirements of var2CSA and several chemokines. ..
  39. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2012
    ..Our studies have already benefited and will continue to facilitate the development of potent LpxC-targeting antibiotics against a broad spectrum of Gram-negative pathogens. ..
  40. Functional analysis of the Francisella capBCA gene locus
    Jing Ren Zhang; Fiscal Year: 2010
    ..tularensis. PGA can be used to develop vaccines against F. tularensis infection if it is placed at the surface of F. tularensis as its counterpart in B. anthracis ..
  41. Development of an antimicrobial peptide therapeutic for Pseudomonas infections
    HELOISE ANNE PEREIRA; Fiscal Year: 2011
    ..Identifying new therapeutics with strong antimicrobial activity and low propensity to induce resistance will be of great public health importance. ..
  42. FOSMIDOMYCIN RESISTANCE IN PLASMODIUM FALCIPARUM
    AUDREY RAGAN ODOM; Fiscal Year: 2013
    ..falciparum genome, and identify new targets for much-needed antimalarial drug development. ..
  43. Gram-Negative Pneumonia and Lipopolysaccharide Binding Protein
    MARK RICHARD HEMMILA; Fiscal Year: 2010
    ..The insights gained from the experiments proposed may lead to new therapeutic interventions for treatment and prevention of bacterial pneumonia. ..
  44. Resolution and repair of acute lung injury by macrophage-derived iNOS
    FRANCO R D'ALESSIO; Fiscal Year: 2011
    ..t. LPS and direct live gram negative bacteria model of ALI. Targets identified in SA 1 and 2 (e.g...
  45. Infant Antibiotic Resistance and Implications for Therapeutic Decision-making
    SHARON MEROPOL; Fiscal Year: 2013
    ..pneumoniae, S. aureus, gram negative enteric bacilli, and ESBL-producing gram negative bacteria and infants'antibiotic exposure. Secondary Objectives: Objective 1...
  46. Regulatory Circuits Controlling Development of Dormant Microbial Cysts
    CARL EUGENE BAUER; Fiscal Year: 2013
    ..Changes in genome wide gene expression will be undertaken with each of these mutants cell lines in order to develop detailed models of the regulatory circuits that are involved in controlling bacterial cyst formation. ..
  47. The Epidemiology of Home Allergens and Asthma
    Diane R Gold; Fiscal Year: 2010
    ..abstract_text> ..
  48. Role of pattern recognition receptors in organic dust-induced airway inflammation
    Jill A Poole; Fiscal Year: 2013
    ....
  49. Structural and biochemical characterization of the OprD membrane protein family
    Bert van den Berg; Fiscal Year: 2011
    ..Besides being important from a fundamental point of view, such knowledge could also be used by pharmaceutical companies to design better drugs against disease-causing bacteria. ..
  50. Hepatic drug metabolism in inflammation
    Romi Ghose; Fiscal Year: 2009
    ..and there is evidence that hepatocytes can be directly targeted by lipopolysaccharide (LPS) from gram negative bacteria resulting in suppression of Cytochrome P450 gene expression...
  51. Resolution and repair of acute lung injury by macrophage-derived iNOS
    FRANCO R D'ALESSIO; Fiscal Year: 2013
    ..t. LPS and direct live gram negative bacteria model of ALI. Targets identified in SA 1 and 2 (e.g...
  52. The Role of Plasmids in Rickettsia Biology
    ULRIKE GERTRUD MUNDERLOH; Fiscal Year: 2013
    ..The results of this research may provide new tools to control Rickettsia infections of humans. ..
  53. Narrow-spectrum Agents Acting against Helicobacter pylori
    Kenneth Coleman; Fiscal Year: 2013
    ..Poor permeability into Gram negative bacteria is another major obstacle for developing broad-spectrum compounds, but H...
  54. Understanding aromatic hydrocarbon uptake as the first step in biodegradation
    Bert van den Berg; Fiscal Year: 2013
    ..The results could potentially be used to design more efficient biodegrader and biocatalyst strains that utilize hydrophobic substrates. ..
  55. Design and mechanistic studies of mimics of antimicrobial peptides
    William F Degrado; Fiscal Year: 2012
    ..Given their broad activity against Gram positive and Gram negative bacteria, AMPs are potential candidates for novel-mechanism iv antibiotics...
  56. IL-1?? as a mediator of maternal infection-induced effects on offspring neuroinfl
    Heping Zhou; Fiscal Year: 2010
    ..Our results may also contribute to the finding of new diagnostic or therapeutic targets for mitigating maternal infection-induced defects in the offspring. ..
  57. GENETIC ANALYSIS OF PROTEIN EXPORT
    Thomas J Silhavy; Fiscal Year: 2013
    ..Multi-drug resistant Gram- negative bacteria are a growing concern. Insights obtained from this work should facilitate the design of novel antibacterial agents. ..
  58. Bacterial Virulence and the Microbiota in Eradication of Enteropathogenic E. Coli
    Gabriel Nunez; Fiscal Year: 2013
    ..Given that A/E pathogens are a major cause of death and morbidity in human populations, this proposal is expected to have a significant and broad impact in the medical field ..
  59. Computational Assembly of Beta Barrel Membrane Protein
    Jie Liang; Fiscal Year: 2013
    ..Extensive mutant studies will be carried out to test whether designed ?-barrel membrane proteins have the intended changes in stability, in oligomerization state, as well as in geometry. ..
  60. ESCHERICHIA COLI INVASION OF BRAIN ENDOTHELIAL CELLS
    Sheng He Huang; Fiscal Year: 2009
    ..These two pathogenicities are dependent on IbeA/E-induced signaling via Vim/LRs. Vim/LRs may be exploited as the potential therapeutic targets for blocking E. coli K1 invasion and PMN transmigration across HBMEC. ..
  61. Activation of Insect Immunity by Gram-negative Bacteria
    Neal Silverman; Fiscal Year: 2013
    ..RYBP is highly conserved, and Aim 3 additionally investigates the role of mouse and human RYBP homologs in mammalian innate immune signaling. ..
  62. ETHANOL REGULATION OF KUPFFER CELL SIGNAL TRANSDUCTION
    Laura Nagy; Fiscal Year: 2009
    ..abstract_text> ..
  63. Bacteriolytic phage enzymes as novel antibacterials against Yersinia pestis
    Ian Molineux; Fiscal Year: 2009
    ..pestis infections. These in vivo studies will parallel those involving lysins using the mouse model of plague infection. ..
  64. Susceptibility Testing, Treatment Choices, and Outcomes of MDR-GNB Infections
    Lisa Maragakis; Fiscal Year: 2009
    ..This research is designed to better understand how providers use information from various microbiology laboratory testing methods to choose treatments and to identify strategies that lead to the best patient outcomes. ..
  65. Genetics and biosynthesis of an O antigen essential for symbiosis
    KENNETH D NOEL; Fiscal Year: 2010
    ..It also is important in other interactions with animal and plant hosts. This property is a type of carbohydrate chain on the surfaces of many bacteria. The question addressed in this work is how this chain is synthesized. ..
  66. MOLECULAR MECHANISMS OF SEPTIC SHOCK
    JACK HAWIGER; Fiscal Year: 2003
    ..The ultimate goal of the proposed studies is to provide a new conceptual framework for reduction of high mortality due to septic shock. ..
  67. Syndecan and Bacterial Translocation in Shock and Trauma
    Carol Wells; Fiscal Year: 2006
    ..faecalis and S. aureus. ..
  68. Detecting Carbapenem and Quinolone Resistance in Klebsiella pneumoniae using PCR/
    Robert A Bonomo; Fiscal Year: 2010
    ..pneumoniae. It is our goal that this method, based upon a novel technology, will form the basis for the implementation of real time infection control and diagnostic paradigms. ..
  69. Detection of Antibiotic Resistance Genes in Bacterial Agents of Hospital-Acquired
    Andrew Levin; Fiscal Year: 2009
    ....
  70. Methylerythritol Phosphate Pathway Inhibitors Targeting Gram-Negative Infections
    CHARLES TESTA; Fiscal Year: 2009
    ....
  71. Methylerythritol Phosphate Pathway-Specific Natural Products as Antibacterials
    CHARLES TESTA; Fiscal Year: 2009
    ..The pathway is not the target of any currently prescribed therapeutic, therefore these compounds and their derivatives are expected to have prolonged utility relative to subsequent generations of antibiotics presently in use. ..
  72. STRUCTURE AND GENETIC CONTROL OF COLICINES
    Donald Helinski; Fiscal Year: 2005
    ..These various studies should contribute to our understanding of the fundamental processes of initiation of DNA replication, DNA segregation, and the dissemination of antibiotic resistance in bacteria. ..