bacillus subtilis

Summary

Summary: A species of gram-positive bacteria that is a common soil and water saprophyte.

Top Publications

  1. ncbi Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis
    Pierre Nicolas
    INRA, UR1077, Mathématique Informatique et Génome, Jouy en Josas, France
    Science 335:1103-6. 2012
  2. ncbi Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism
    Joerg Martin Buescher
    Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
    Science 335:1099-103. 2012
  3. ncbi Bistability, epigenetics, and bet-hedging in bacteria
    Jan Willem Veening
    Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    Annu Rev Microbiol 62:193-210. 2008
  4. pmc Bet-hedging and epigenetic inheritance in bacterial cell development
    Jan Willem Veening
    Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, and Theoretical Biology Group, Centre for Ecological and Evolutionary Studies, University of Groningen, 9751 NN, Haren, The Netherlands
    Proc Natl Acad Sci U S A 105:4393-8. 2008
  5. pmc Identification of regulatory RNAs in Bacillus subtilis
    Irnov Irnov
    Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9038, USA
    Nucleic Acids Res 38:6637-51. 2010
  6. pmc Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A
    Masaya Fujita
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 19:2236-44. 2005
  7. ncbi Multiple sigma subunits and the partitioning of bacterial transcription space
    Tanja M Gruber
    Department of Microbiology and Immunology, University of California, Genentech Hall, 600 16th St, San Francisco, San Francisco, California 94143, USA
    Annu Rev Microbiol 57:441-66. 2003
  8. pmc The transcriptionally active regions in the genome of Bacillus subtilis
    Simon Rasmussen
    Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark
    Mol Microbiol 73:1043-57. 2009
  9. pmc Crystal structure of Hfq from Bacillus subtilis in complex with SELEX-derived RNA aptamer: insight into RNA-binding properties of bacterial Hfq
    Tatsuhiko Someya
    Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba shi, Ibaraki 305 8572, Japan
    Nucleic Acids Res 40:1856-67. 2012
  10. pmc Recent progress in Bacillus subtilis sporulation
    Douglas Higgins
    Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, New York, NY, USA
    FEMS Microbiol Rev 36:131-48. 2012

Detail Information

Publications367 found, 100 shown here

  1. ncbi Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis
    Pierre Nicolas
    INRA, UR1077, Mathématique Informatique et Génome, Jouy en Josas, France
    Science 335:1103-6. 2012
    ..Here, we report the transcriptomes of Bacillus subtilis exposed to a wide range of environmental and nutritional conditions that the organism might encounter in ..
  2. ncbi Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism
    Joerg Martin Buescher
    Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
    Science 335:1099-103. 2012
    ..For nutritional shifts between two preferred carbon sources of Bacillus subtilis, we combined statistical and model-based data analyses of dynamic transcript, protein, and metabolite ..
  3. ncbi Bistability, epigenetics, and bet-hedging in bacteria
    Jan Willem Veening
    Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    Annu Rev Microbiol 62:193-210. 2008
    ..This suggests that microbial cells employ bet-hedging strategies to maximize survival. Here, we discuss the possible roles of interlinked bistable networks, epigenetic inheritance, and bet-hedging in bacteria...
  4. pmc Bet-hedging and epigenetic inheritance in bacterial cell development
    Jan Willem Veening
    Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, and Theoretical Biology Group, Centre for Ecological and Evolutionary Studies, University of Groningen, 9751 NN, Haren, The Netherlands
    Proc Natl Acad Sci U S A 105:4393-8. 2008
    Upon nutritional limitation, the bacterium Bacillus subtilis has the capability to enter the irreversible process of sporulation...
  5. pmc Identification of regulatory RNAs in Bacillus subtilis
    Irnov Irnov
    Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9038, USA
    Nucleic Acids Res 38:6637-51. 2010
    ..this analysis we catalog, by a deep sequencing-based approach, both classes of regulatory RNA candidates for Bacillus subtilis, the model microorganism for Firmicutes...
  6. pmc Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A
    Masaya Fujita
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 19:2236-44. 2005
    The transcription factor Spo0A is a master regulator for entry into sporulation in the bacterium Bacillus subtilis, but it has been uncertain whether activation of Spo0A is sufficient to trigger development...
  7. ncbi Multiple sigma subunits and the partitioning of bacterial transcription space
    Tanja M Gruber
    Department of Microbiology and Immunology, University of California, Genentech Hall, 600 16th St, San Francisco, San Francisco, California 94143, USA
    Annu Rev Microbiol 57:441-66. 2003
    ..We use the sigma repertoire of Escherichia coli, Bacillus subtilis, Streptomyces coelicolor, and cyanobacteria to illustrate the different strategies utilized to organize ..
  8. pmc The transcriptionally active regions in the genome of Bacillus subtilis
    Simon Rasmussen
    Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark
    Mol Microbiol 73:1043-57. 2009
    ..Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays...
  9. pmc Crystal structure of Hfq from Bacillus subtilis in complex with SELEX-derived RNA aptamer: insight into RNA-binding properties of bacterial Hfq
    Tatsuhiko Someya
    Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba shi, Ibaraki 305 8572, Japan
    Nucleic Acids Res 40:1856-67. 2012
    ..However, functional and structural information about Bacillus subtilis Hfq (BsHfq) including the RNA sequences that specifically bind to it remain unknown...
  10. pmc Recent progress in Bacillus subtilis sporulation
    Douglas Higgins
    Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, New York, NY, USA
    FEMS Microbiol Rev 36:131-48. 2012
    The Gram-positive bacterium Bacillus subtilis can initiate the process of sporulation under conditions of nutrient limitation...
  11. ncbi Double-strand break repair in bacteria: a view from Bacillus subtilis
    Silvia Ayora
    Departmento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, Cantoblanco, Madrid, Spain
    FEMS Microbiol Rev 35:1055-81. 2011
    ..In Bacillus subtilis, this response can be subdivided into five general acts: (1) recognition of the break site(s) and formation ..
  12. pmc Broadly heterogeneous activation of the master regulator for sporulation in Bacillus subtilis
    Arnaud Chastanet
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 107:8486-91. 2010
    ..system for investigating how developmental regulatory networks determine cell fate is spore formation in Bacillus subtilis. The master regulator for sporulation is Spo0A, which is activated by phosphorylation via a phosphorelay ..
  13. pmc RNase Y, a novel endoribonuclease, initiates riboswitch turnover in Bacillus subtilis
    Karen Shahbabian
    Institut de Biologie Physico Chimique, CNRS UPR9073, affiliated with Université de Paris 7 Denis Diderot, Paris, France
    EMBO J 28:3523-33. 2009
    ..generated by premature transcription termination of S-adenosylmethionine (SAM)-dependent riboswitches in Bacillus subtilis. An essential protein of earlier unknown function, YmdA, was identified as a novel endoribonuclease (now ..
  14. ncbi Whole genome sequencing of meticillin-resistant Staphylococcus aureus
    M Kuroda
    Hiramatsu, Department of Bacteriology, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan
    Lancet 357:1225-40. 2001
    ..Investigation of many newly identified gene products, including the 70 putative virulence factors, will greatly improve our understanding of the biology of staphylococci and the processes of infectious diseases caused by S aureus...
  15. pmc Bacterial programmed cell death and multicellular behavior in bacteria
    Hanna Engelberg-Kulka
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    PLoS Genet 2:e135. 2006
    ..The second program is found in Bacillus subtilis, in which the skf and sdp operons mediate the death of a subpopulation of sporulating bacterial cells...
  16. pmc Localisation of DivIVA by targeting to negatively curved membranes
    Rok Lenarcic
    Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne, UK
    EMBO J 28:2272-82. 2009
    ..DivIVA is specifically targeted to cell division sites and cell poles. In Bacillus subtilis, DivIVA helps to localise other proteins, such as the conserved cell division inhibitor proteins, MinC/MinD, ..
  17. pmc Transcriptional landscape estimation from tiling array data using a model of signal shift and drift
    Pierre Nicolas
    INRA, Mathématique Informatique et Génome UR1077, 78350 Jouy en Josas, France
    Bioinformatics 25:2341-7. 2009
    ..The statistical models currently used to handle these data remain however very simple, the most popular being the piecewise constant Gaussian model with a fixed number of breakpoints...
  18. pmc Amyloid fibers provide structural integrity to Bacillus subtilis biofilms
    Diego Romero
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:2230-4. 2010
    b>Bacillus subtilis forms biofilms whose constituent cells are held together by an extracellular matrix. Previous studies have shown that the protein TasA and an exopolysaccharide are the main components of the matrix...
  19. pmc An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis
    Jamie Richards
    Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Microbiology, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Mol Cell 43:940-9. 2011
    ..Remarkably, no homolog of this key endonuclease is present in many bacterial species, such as Bacillus subtilis and various pathogens. Here, we report that the degradation of primary transcripts in B...
  20. ncbi Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC
    Tsutomu Katayama
    Department of Molecular Biology, Kyushu University Graduate School of Pharmaceutical Sciences, Fukuoka 812 8582, Japan
    Nat Rev Microbiol 8:163-70. 2010
    ..Here, we describe the regulation of DnaA, one of the central proteins involved in bacterial DNA replication, by these factors in Escherichia coli, Bacillus subtilis and Caulobacter crescentus.
  21. pmc A metabolic sensor governing cell size in bacteria
    Richard B Weart
    Department of Biology, Washington University, St Louis, MO 63130, USA
    Cell 130:335-47. 2007
    ..We report the identification of a metabolic sensor that couples nutritional availability to division in Bacillus subtilis. A key component of this sensor is an effector, UgtP, which localizes to the division site in a nutrient-..
  22. pmc Recruitment of SMC by ParB-parS organizes the origin region and promotes efficient chromosome segregation
    Nora L Sullivan
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell 137:697-707. 2009
    ..In Bacillus subtilis, parS sites are bound by the partitioning protein Spo0J (ParB), and we show here that Spo0J recruits the SMC ..
  23. pmc High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis
    Masaya Fujita
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave, Cambridge, MA 02138, USA
    J Bacteriol 187:1357-68. 2005
    The master regulator for entry into sporulation in Bacillus subtilis is the response regulator Spo0A, which directly governs the expression of about 121 genes...
  24. ncbi Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria
    Julia Domínguez-Escobar
    Max Planck Institute of Biochemistry, Martinsried, Germany
    Science 333:225-8. 2011
    ..microscopy to visualize the dynamic relation between MreB isoforms and cell wall synthesis in live Bacillus subtilis cells. During exponential growth, MreB proteins did not form helical structures...
  25. pmc Biofilms
    Daniel Lopez
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cold Spring Harb Perspect Biol 2:a000398. 2010
    ..to give an overview of how several organisms form biofilms: Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus...
  26. ncbi The Spo0A regulon of Bacillus subtilis
    Virginie Molle
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Microbiol 50:1683-701. 2003
    The master regulator for entry into sporulation in Bacillus subtilis is the DNA-binding protein Spo0A, which has been found to influence, directly or indirectly, the expression of over 500 genes during the early stages of development...
  27. ncbi A master regulator for biofilm formation by Bacillus subtilis
    Daniel B Kearns
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Mol Microbiol 55:739-49. 2005
    Wild strains of Bacillus subtilis are capable of forming architecturally complex communities of cells known as biofilms...
  28. pmc Ribonucleases J1 and J2: two novel endoribonucleases in B.subtilis with functional homology to E.coli RNase E
    Sergine Even
    CNRS UPR9073, IBPC 13 rue P et M Curie, 75005 Paris, France
    Nucleic Acids Res 33:2141-52. 2005
    ..In this paper, we report the purification and identification by mass spectrometry in Bacillus subtilis of two paralogous endoribonucleases, here named RNases J1 and J2, which share functional homologies with ..
  29. pmc Three essential ribonucleases-RNase Y, J1, and III-control the abundance of a majority of Bacillus subtilis mRNAs
    Sylvain Durand
    CNRS UPR 9073, University Paris Diderot, Sorbonne Paris Cité, Institut de Biologie Physico Chimique, Paris, France
    PLoS Genet 8:e1002520. 2012
    b>Bacillus subtilis possesses three essential enzymes thought to be involved in mRNA decay to varying degrees, namely RNase Y, RNase J1, and RNase III...
  30. ncbi Bistability in bacteria
    David Dubnau
    Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Mol Microbiol 61:564-72. 2006
    ..Here we consider four cases of bistability, three from Bacillus subtilis and one from Escherichia coli, with an emphasis on random switching mechanisms that generate alternative ..
  31. pmc Novel activities of glycolytic enzymes in Bacillus subtilis: interactions with essential proteins involved in mRNA processing
    Fabian M Commichau
    Department of General Microbiology, Georg August University Gottingen, Grisebachstrasse 8, D 37077 Gottingen, Germany
    Mol Cell Proteomics 8:1350-60. 2009
    ..In this study, a screening for in vivo interaction partners of glycolytic enzymes of the soil bacterium Bacillus subtilis was used to provide a rationale for essentiality of glycolytic enzymes...
  32. pmc From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later
    Valerie Barbe
    CEA, Institut de Génomique, Genoscope, 2 rue Gaston Cremieux, 91057 Evry, France
    Microbiology 155:1758-75. 2009
    ..The bacterial domain is split into large branches, among which the Firmicutes occupy a considerable space. Bacillus subtilis has been the model of Firmicutes for decades and its genome has been a reference for more than 10 years...
  33. pmc Combined effect of improved cell yield and increased specific productivity enhances recombinant enzyme production in genome-reduced Bacillus subtilis strain MGB874
    Kenji Manabe
    Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321 3497, Japan
    Appl Environ Microbiol 77:8370-81. 2011
    ..We reported previously that the novel Bacillus subtilis strain MGB874, which was derived from strain 168 and has a total genomic deletion of 874 kb (20...
  34. ncbi 5'-to-3' exoribonuclease activity in bacteria: role of RNase J1 in rRNA maturation and 5' stability of mRNA
    Nathalie Mathy
    CNRS UPR 9073 affiliated with Université de Paris 7 Denis Diderot, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris
    Cell 129:681-92. 2007
    ..e. 3' to 5'. Here we show that maturation of the 5' side of Bacillus subtilis 16S ribosomal RNA occurs via a 5'-to-3' exonucleolytic pathway, catalyzed by the widely distributed ..
  35. pmc Biochemical characterization of ribosome assembly GTPase RbgA in Bacillus subtilis
    David Achila
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824, USA
    J Biol Chem 287:8417-23. 2012
    The ribosome biogenesis GTPase A protein RbgA is involved in the assembly of the large ribosomal subunit in Bacillus subtilis, and homologs of RbgA are implicated in the biogenesis of mitochondrial, chloroplast, and cytoplasmic ribosomes ..
  36. pmc Bistability in the Bacillus subtilis K-state (competence) system requires a positive feedback loop
    Hédia Maamar
    Public Health Research Institute, 225 Warren Street, Newark NJ 07103, USA
    Mol Microbiol 56:615-24. 2005
    ..of the transcriptional activator ComK occurs in 10-20% of the cells in stationary phase cultures of Bacillus subtilis strain 168...
  37. ncbi The identity of the transcription +1 position is crucial for changes in gene expression in response to amino acid starvation in Bacillus subtilis
    Libor Krásný
    Laboratory of Molecular Genetics of Bacteria, Institute of Microbiology ASCR, Videnska 1083, Prague 142 20, Czech Republic
    Mol Microbiol 69:42-54. 2008
    ..promoters of genes that are up-/downregulated in response to amino acid starvation (stringent response) in Bacillus subtilis. Upregulated promoters initiate mostly with ATP and downregulated promoters with GTP...
  38. pmc Coupled, circumferential motions of the cell wall synthesis machinery and MreB filaments in B. subtilis
    Ethan C Garner
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Science 333:222-5. 2011
    ..the dynamics of MreB and the cell wall elongation machinery using high-precision particle tracking in Bacillus subtilis. We found that MreB and the elongation machinery moved circumferentially around the cell, perpendicular to ..
  39. ncbi What is the role of RNase J in mRNA turnover?
    Ciaran Condon
    Institut de Biologie Physico Chimique, Paris, France
    RNA Biol 7:316-21. 2010
    ..the paralogous ribonucleases J1 and J2 has been a major advance in the study of RNA maturation and decay in Bacillus subtilis and related organisms...
  40. ncbi Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression
    P Stragier
    Institut de Microbiologie, Universite Paris Sud, Orsay, France
    Cell 52:697-704. 1988
    Sporulation of Bacillus subtilis is a primitive example of coupling between morphological changes and timing of gene expression during development...
  41. pmc Bacillus subtilis CodY represses early-stationary-phase genes by sensing GTP levels
    M Ratnayake-Lecamwasam
    Tufts University School of Medicine, Department of Molecular Biology and Microbiology, Boston, Massachusetts 02111, USA
    Genes Dev 15:1093-103. 2001
    CodY, a highly conserved protein in the low G + C, gram-positive bacteria, regulates the expression of many Bacillus subtilis genes that are induced as cells make the transition from rapid exponential growth to stationary phase and ..
  42. ncbi Carbon catabolite control of the metabolic network in Bacillus subtilis
    Yasutaro Fujita
    Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Hiroshima, Japan
    Biosci Biotechnol Biochem 73:245-59. 2009
    ..In Bacillus subtilis and close relatives, global regulation of carbon catabolite control occurs on the binding of the complex of ..
  43. ncbi Spo0J regulates the oligomeric state of Soj to trigger its switch from an activator to an inhibitor of DNA replication initiation
    Graham Scholefield
    Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4AX, UK
    Mol Microbiol 79:1089-100. 2011
    ..The Bacillus subtilis ATPase Soj is a dynamic protein that regulates DNA replication initiation by either inhibiting or activating ..
  44. pmc Functional microdomains in bacterial membranes
    Daniel Lopez
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 24:1893-902. 2010
    ..The orchestration of physiological processes in microdomains may be a more widespread feature of membranes than previously appreciated...
  45. ncbi Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals
    P Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, 06030 3305, USA
    J Appl Microbiol 101:514-25. 2006
    ..SASP are also important in spore resistance to dry heat, as is DNA repair in spore outgrowth, as Bacillus subtilis spores are killed by dry heat via DNA damage. Both UV and gamma-radiation also kill spores via DNA damage...
  46. ncbi The speed of RNA transcription and metabolite binding kinetics operate an FMN riboswitch
    J Kenneth Wickiser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Mol Cell 18:49-60. 2005
    ..A flavin mononucleotide (FMN)-dependent riboswitch from the ribDEAHT operon of Bacillus subtilis uses a transcription termination mechanism wherein formation of an RNA-FMN complex causes formation of an ..
  47. ncbi The dhb operon of Bacillus subtilis encodes the biosynthetic template for the catecholic siderophore 2,3-dihydroxybenzoate-glycine-threonine trimeric ester bacillibactin
    J J May
    Department of Chemistry, Philipps Universitat Marburg, D 35032 Marburg, Germany
    J Biol Chem 276:7209-17. 2001
    b>Bacillus subtilis was reported to produce the catecholic siderophore itoic acid (2,3-dihydroxybenzoate (DHB)-glycine) in response to iron deprivation...
  48. pmc Spx-dependent global transcriptional control is induced by thiol-specific oxidative stress in Bacillus subtilis
    Shunji Nakano
    Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006, USA
    Proc Natl Acad Sci U S A 100:13603-8. 2003
    The Spx protein of Bacillus subtilis represses activator-stimulated transcription by interacting with the C-terminal domain of RNA polymerase (RNAP) alpha subunit...
  49. ncbi Maturation of the 5' end of Bacillus subtilis 16S rRNA by the essential ribonuclease YkqC/RNase J1
    Robert A Britton
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
    Mol Microbiol 63:127-38. 2007
    ..Here we show that the maturation of Bacillus subtilis 16S rRNA is also a two-step process and that the enzyme responsible for the generation of the mature 5' end ..
  50. ncbi Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles
    Ki Young Yoon
    Department of Mechanical Engineering, Yonsei University, 120 749 Seoul, Republic of Korea
    Sci Total Environ 373:572-5. 2007
    ..evaluate the antimicrobial characteristics of silver and copper nanoparticles against Escherichia coli and Bacillus subtilis. Reaction of copper nanoparticles of 100 nm with B. subtilis showed the highest susceptibility (Z=0...
  51. ncbi The complete genome sequence of the gram-positive bacterium Bacillus subtilis
    F Kunst
    Institut Pasteur, Unité de Biochimie Microbienne, Paris, France
    Nature 390:249-56. 1997
    b>Bacillus subtilis is the best-characterized member of the Gram-positive bacteria. Its genome of 4,214,810 base pairs comprises 4,100 protein-coding genes...
  52. pmc Differential expression of two paralogous genes of Bacillus subtilis encoding single-stranded DNA binding protein
    Cordula Lindner
    Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, NL 9751 NN Haren, The Netherlands
    J Bacteriol 186:1097-105. 2004
    The Bacillus subtilis genome comprises two paralogous single-stranded DNA binding protein (SSB) genes, ssb and ywpH, which show distinct expression patterns...
  53. ncbi A minimal Tat system from a gram-positive organism: a bifunctional TatA subunit participates in discrete TatAC and TatA complexes
    James P Barnett
    Department of Biological Sciences, University of Warwick, Coventry, United Kingdom
    J Biol Chem 283:2534-42. 2008
    ..Here, we have studied Tat complexes encoded by the tatAdCd genes of Bacillus subtilis. Expression of tatAdCd in an Escherichia coli tat null mutant results in efficient export of a large, ..
  54. ncbi A glycine-dependent riboswitch that uses cooperative binding to control gene expression
    Maumita Mandal
    Department of Molecular, Cellular, and Developmental Biology, Yale University, Post Office Box 208103, New Haven, CT 06520 8103, USA
    Science 306:275-9. 2004
    ..A representative of these glycine-sensing RNAs from Bacillus subtilis operates as a rare genetic on switch for the gcvT operon, which codes for proteins that form the glycine ..
  55. pmc Comparative study between transcriptionally- and translationally-acting adenine riboswitches reveals key differences in riboswitch regulatory mechanisms
    Jean François Lemay
    Groupe ARN RNA Group, Departement de Biologie, Faculte des Sciences, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
    PLoS Genet 7:e1001278. 2011
    ..Although they both bind adenine, the adenine riboswitches of Bacillus subtilis and Vibrio vulnificus differ by controlling transcription and translation, respectively...
  56. ncbi Cannibalism by sporulating bacteria
    Jose E Gonzalez-Pastor
    Department of Molecular and Cellular Biology, The Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Science 301:510-3. 2003
    Spore formation by the bacterium Bacillus subtilis is an elaborate developmental process that is triggered by nutrient limitation...
  57. ncbi Regulation of noise in the expression of a single gene
    Ertugrul M Ozbudak
    Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Genet 31:69-73. 2002
    ..the rates of transcription and translation of a single fluorescent reporter gene in the chromosome of Bacillus subtilis, and we quantitatively measured the resulting changes in the phenotypic noise characteristics...
  58. ncbi Coordination of cell division and chromosome segregation by a nucleoid occlusion protein in Bacillus subtilis
    Ling Juan Wu
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Cell 117:915-25. 2004
    ..We have identified a specific effector of nucleoid occlusion in Bacillus subtilis, Noc (YyaA), as an inhibitor of division that is also a nonspecific DNA binding protein...
  59. ncbi A major protein component of the Bacillus subtilis biofilm matrix
    Steven S Branda
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 59:1229-38. 2006
    ..present evidence from scanning electron microscopy showing that a wild strain of the Gram positive bacterium Bacillus subtilis builds such a matrix...
  60. pmc Cooperative and directional folding of the preQ1 riboswitch aptamer domain
    Jun Feng
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Am Chem Soc 133:4196-9. 2011
    ..This kinetically efficient folding mechanism suggests a fast ligand-binding response in competition with RNA elongation...
  61. ncbi Solution NMR structure of the TatA component of the twin-arginine protein transport system from gram-positive bacterium Bacillus subtilis
    Yunfei Hu
    Beijing NMR Center, Peking University, Beijing 100871, China
    J Am Chem Soc 132:15942-4. 2010
    ..Herein, we report the solution structure of the TatA(d) protein from Bacillus subtilis by NMR spectroscopy, the first structure of the Tat system at atomic resolution...
  62. pmc Initiation of decay of Bacillus subtilis rpsO mRNA by endoribonuclease RNase Y
    Shiyi Yao
    Department of Pharmacology and Systems Therapeutics, Box 1603, Mount Sinai School of Medicine of New York University, New York, NY 10029, USA
    J Bacteriol 192:3279-86. 2010
    ..monocistronic mRNA that encodes ribosomal protein S15, was used to study aspects of mRNA decay initiation in Bacillus subtilis. Decay of rpsO mRNA in a panel of 3'-to-5' exoribonuclease mutants was analyzed using a 5'-proximal ..
  63. ncbi Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression
    Shanshan Li
    Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
    Appl Microbiol Biotechnol 91:577-89. 2011
    In the present work, Bacillus subtilis was engineered as the cell factory for isobutanol production due to its high tolerance to isobutanol...
  64. pmc The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks
    Audrey Costes
    Laboratoire de Microbiologie et Genetique Moleculaires, Universite de Toulouse, Centre National de la Recherche Scientifique, LMGM UMR5100, Toulouse, France
    PLoS Genet 6:e1001238. 2010
    We have investigated in vivo the role of the carboxy-terminal domain of the Bacillus subtilis Single-Stranded DNA Binding protein (SSB(Cter)) as a recruitment platform at active chromosomal forks for many proteins of the genome ..
  65. ncbi Defining the structure of the general stress regulon of Bacillus subtilis using targeted microarray analysis and random forest classification
    Priyanka Nannapaneni
    Interfakultäres Institut für Genetik und Funktionelle Genomforschung, Ernst Moritz Arndt Universitat Greifswald, Germany
    Microbiology 158:696-707. 2012
    The structure of the SigB-dependent general stress regulon of Bacillus subtilis has previously been characterized by proteomics approaches as well as DNA array-based expression studies...
  66. pmc YycH and YycI interact to regulate the essential YycFG two-component system in Bacillus subtilis
    Hendrik Szurmant
    Division of Cellular Biology, Mail Code MEM 116, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Bacteriol 189:3280-9. 2007
    The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability...
  67. ncbi Messenger RNA decay and maturation in Bacillus subtilis
    David H Bechhofer
    Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine of New York University, New York, NY 10029, USA
    Prog Mol Biol Transl Sci 85:231-73. 2009
    Our understanding of the ribonucleases that act to process and turn over RNA in Bacillus subtilis, a model Gram-positive organism, has increased greatly in recent years. This chapter discusses characteristics of B...
  68. ncbi Surfactin from Bacillus subtilis displays anti-proliferative effect via apoptosis induction, cell cycle arrest and survival signaling suppression
    Seo Young Kim
    Department of Genetic Engineering, Sungkyunkwan University, Suwon 440 746, Republic of Korea
    FEBS Lett 581:865-71. 2007
    ..Therefore, our data suggest that surfactin may have anti-cancer properties as a result of its ability to downregulate the cell cycle and suppress its survival...
  69. pmc A novel factor controlling bistability in Bacillus subtilis: the YmdB protein affects flagellin expression and biofilm formation
    Christine Diethmaier
    Department of General Microbiology, Georg August University Gottingen, Grisebachstr 8, 37077 Gottingen, Germany
    J Bacteriol 193:5997-6007. 2011
    Cells of Bacillus subtilis can either be motile or sessile, depending on the expression of mutually exclusive sets of genes that are required for flagellum or biofilm formation, respectively...
  70. pmc RNase Y in Bacillus subtilis: a Natively disordered protein that is the functional equivalent of RNase E from Escherichia coli
    Martin Lehnik-Habrink
    Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University Gottingen, Grisebachstr 8, D 37077 Gottingen, Germany
    J Bacteriol 193:5431-41. 2011
    ..In Escherichia coli, the RNA degradosome is assembled around the essential endoribonuclease E. In Bacillus subtilis, the recently discovered essential endoribonuclease RNase Y is involved in the initiation of RNA degradation...
  71. pmc Rapid changes in gene expression: DNA determinants of promoter regulation by the concentration of the transcription initiating NTP in Bacillus subtilis
    Ludek Sojka
    Laboratory of Molecular Genetics of Bacteria, Institute of Microbiology, Academy of Sciences of the Czech Republic
    Nucleic Acids Res 39:4598-611. 2011
    ..Ribosomal RNA (rRNA) promoters from Gram-positive Bacillus subtilis are regulated by the concentration of their iNTP...
  72. pmc Regulatory overlap and functional redundancy among Bacillus subtilis extracytoplasmic function sigma factors
    Thorsten Mascher
    Department of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853 8101, USA
    J Bacteriol 189:6919-27. 2007
    b>Bacillus subtilis encodes seven extracytoplasmic function (ECF) sigma factors that regulate partially overlapping regulons related to cell envelope homeostasis and antibiotic resistance...
  73. pmc DBTBS: a database of transcriptional regulation in Bacillus subtilis containing upstream intergenic conservation information
    Nicolas Sierro
    Human Genome Center, The Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo, Japan
    Nucleic Acids Res 36:D93-6. 2008
    DBTBS, first released in 1999, is a reference database on transcriptional regulation in Bacillus subtilis, summarizing the experimentally characterized transcription factors, their recognition sequences and the genes they regulate...
  74. pmc Parallel pathways of repression and antirepression governing the transition to stationary phase in Bacillus subtilis
    Allison V Banse
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 105:15547-52. 2008
    The AbrB protein of the spore-forming bacterium Bacillus subtilis is a repressor of numerous genes that are switched on during the transition from the exponential to the stationary phase of growth...
  75. pmc SAM recognition and conformational switching mechanism in the Bacillus subtilis yitJ S box/SAM-I riboswitch
    Changrui Lu
    Department of Molecular Biology and Genetics, 251 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    J Mol Biol 404:803-18. 2010
    ..We report here the 3.0-Å crystal structure of the aptamer domain of the Bacillus subtilis yitJ S-box (SAM-I) riboswitch bound to S-adenosyl-L-methionine (SAM)...
  76. ncbi The PerR transcription factor senses H2O2 by metal-catalysed histidine oxidation
    Jin Won Lee
    Department of Microbiology, Cornell University, Ithaca, New York 14853, USA
    Nature 440:363-7. 2006
    ..b>Bacillus subtilis PerR is the prototype for a widespread family of metal-dependent peroxide sensors that regulate inducible ..
  77. pmc Essential Bacillus subtilis genes
    K Kobayashi
    Graduate School of Information Science, Nara Institute of Science and Technology, Nara 630 0101, Japan
    Proc Natl Acad Sci U S A 100:4678-83. 2003
    ..set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes...
  78. ncbi Dynamic control of the DNA replication initiation protein DnaA by Soj/ParA
    Heath Murray
    Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK
    Cell 135:74-84. 2008
    ..These results suggest that the pleiotropy manifested by chromosomal parABS mutations could be the indirect effects of a primary activity regulating DNA replication initiation...
  79. ncbi Inferring the transcriptional network of Bacillus subtilis
    Abeer Fadda
    Department of Microbial and Molecular Systems, KULeuven, Kasteelpark Arenberg 20, 3001 Heverlee, Belgium
    Mol Biosyst 5:1840-52. 2009
    ..In this study, we infer the transcriptional network of the Gram-positive model organism, Bacillus subtilis. We use a data integration workflow, exploiting both motif and expression data, towards the generation of ..
  80. pmc A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments
    Ishita M Shah
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Cell 135:486-96. 2008
    ..into the extracellular milieu, and we report here that these muropeptides are potent germinants of dormant Bacillus subtilis spores...
  81. pmc Abundance of type I toxin-antitoxin systems in bacteria: searches for new candidates and discovery of novel families
    Elizabeth M Fozo
    Eunice Kennedy Shriver National Institute of Child Health and Human Development and National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA
    Nucleic Acids Res 38:3743-59. 2010
    ..We detected sRNAs for three predicted toxins from enterohemorrhagic Escherichia coli and Bacillus subtilis, and showed that two of the respective proteins indeed are toxic when overexpressed...
  82. pmc The RecU Holliday junction resolvase acts at early stages of homologous recombination
    Cristina Cañas
    Departmento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, C Darwin 3, Campus Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Nucleic Acids Res 36:5242-9. 2008
    ..The coordination of the synaptic step with the late resolution step is poorly understood. Bacillus subtilis RecU catalyzes resolution of HJs, and biochemical evidence suggests that it might modulate RecA...
  83. pmc Complexity in bacterial cell-cell communication: quorum signal integration and subpopulation signaling in the Bacillus subtilis phosphorelay
    Ilka B Bischofs
    Department of Bioengineering, University of California, 311 Hildebrand Hall, MC 5230, Berkeley, CA 94704 3224, USA
    Proc Natl Acad Sci U S A 106:6459-64. 2009
    ..In Bacillus subtilis, several Phr signals are integrated in sporulation phosphorelay signal transduction...
  84. ncbi A novel component of the division-site selection system of Bacillus subtilis and a new mode of action for the division inhibitor MinCD
    Marc Bramkamp
    Institute for Biochemistry, University of Cologne, Zülpicher Str 47, D 50674, Germany
    Mol Microbiol 70:1556-69. 2008
    ..In Bacillus subtilis recruitment of the division inhibitor MinCD to cell poles depends on DivIVA, and these proteins were thought ..
  85. pmc Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo
    Wiep Klaas Smits
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Microbiol 75:452-61. 2010
    The essential proteins DnaB, DnaD and DnaI of Bacillus subtilis are required for initiation, but not elongation, of DNA replication, and for replication restart at stalled forks...
  86. ncbi Structure and mechanism of a metal-sensing regulatory RNA
    Charles E Dann
    Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Cell 130:878-92. 2007
    ..Given the distribution of this RNA element, it may constitute a common mode for bacterial metal ion regulation, and its discovery suggests the possibility of additional RNA-based metal sensors in modern and primordial organisms...
  87. ncbi Architecture-dependent noise discriminates functionally analogous differentiation circuits
    Tolga Cagatay
    Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Cell 139:512-22. 2009
    ..To investigate this problem, we compared the Bacillus subtilis circuit that regulates differentiation into the competence state to an engineered circuit with an ..
  88. ncbi Production of antimicrobial substances by Bacillus subtilis LFE-1, B. firmus HO-1 and B. licheniformis T6-5 isolated from an oil reservoir in Brazil
    E Korenblum
    Departamento de Microbiologia Geral, Instituto de Microbiologia Prof Paulo de Góes IMPPG, Centro de Ciencias da Saude CCS, Universidade Federal do Rio de Janeiro UFRJ, Brazil
    J Appl Microbiol 98:667-75. 2005
    ..Three strains, Bacillus subtilis (LFE-1), Bacillus firmus (H2O-1) and Bacillus licheniformis (T6-5), were selected due to their ability to ..
  89. ncbi SigB-dependent general stress response in Bacillus subtilis and related gram-positive bacteria
    Michael Hecker
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, Greifswald 17489, Germany
    Annu Rev Microbiol 61:215-36. 2007
    One of the strongest and most noticeable responses of Bacillus subtilis cells to a range of stress and starvation stimuli is the dramatic induction of about 150 SigB-dependent general stress genes...
  90. ncbi The crystal structure of Bacillus subtilis lipase: a minimal alpha/beta hydrolase fold enzyme
    G van Pouderoyen
    Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands
    J Mol Biol 309:215-26. 2001
    The X-ray structure of the lipase LipA from Bacillus subtilis has been determined at 1.5 A resolution. It is the first structure of a member of homology family 1.4 of bacterial lipases...
  91. pmc Role of the Fur regulon in iron transport in Bacillus subtilis
    Juliane Ollinger
    Department of Microbiology, Cornell University, Ithaca, NY 14853 8101, USA
    J Bacteriol 188:3664-73. 2006
    The Bacillus subtilis ferric uptake regulator (Fur) protein mediates the iron-dependent repression of at least 20 operons encoding approximately 40 genes...
  92. ncbi Multiple protein-aspartate phosphatases provide a mechanism for the integration of diverse signals in the control of development in B. subtilis
    M Perego
    Dipartimento Farmaceutico, Universita degli Studi di Parma, Italy
    Cell 79:1047-55. 1994
    ..The integration of diverse environmental signals that affect the initiation of sporulation likely occurs through the competition between opposing activities of protein kinases and protein phosphatases...
  93. pmc Carbon catabolite repression in Bacillus subtilis: quantitative analysis of repression exerted by different carbon sources
    Kalpana D Singh
    Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University Gottingen, Grisebachstr 8, D 37077 Gottingen, Germany
    J Bacteriol 190:7275-84. 2008
    ..In Bacillus subtilis, this carbon catabolite repression (CCR) is achieved by the global transcription regulator CcpA, whose ..
  94. ncbi pSM19035-encoded zeta toxin induces stasis followed by death in a subpopulation of cells
    Virginia S Lioy
    Department of Microbial Biotechnology, Centro Nacional de Biotecnologia, CSIC, 28049 Madrid, Spain
    Microbiology 152:2365-79. 2006
    ..Accumulation of zeta toxin free of epsilon antitoxin induced loss of cell proliferation in both Bacillus subtilis and Escherichia coli cells. Induction of a zeta variant (zetaY83C) triggered stasis, in which B...
  95. pmc Partial penetrance facilitates developmental evolution in bacteria
    Avigdor Eldar
    Howard Hughes Medical Institute and Division of Biology and Department of Applied Physics, California Institute of Technology, Pasadena, California 91125, USA
    Nature 460:510-4. 2009
    ..Here we identify a stochastic cell fate determination process that operates in Bacillus subtilis sporulation mutants and show how it allows genetic control of the penetrance of multiple fates...
  96. pmc Noc protein binds to specific DNA sequences to coordinate cell division with chromosome segregation
    Ling Juan Wu
    Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle upon Tyne, UK
    EMBO J 28:1940-52. 2009
    Coordination of chromosome segregation and cytokinesis is crucial for efficient cell proliferation. In Bacillus subtilis, the nucleoid occlusion protein Noc protects the chromosomes by associating with the chromosome and preventing cell ..
  97. ncbi Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis
    Hideaki Nanamiya
    Cell Free Science and Technology Research Center, Ehime University, Bunkyo cho, Matsuyama 790 8577, Japan
    Mol Microbiol 67:291-304. 2008
    ..and SpoT, have been identified and characterized in Escherichia coli, whereas Gram-positive bacteria such as Bacillus subtilis have been thought to possess only a single RelA-SpoT enzyme...
  98. pmc Noise in gene expression determines cell fate in Bacillus subtilis
    Hédia Maamar
    Public Health Research Institute Center, New Jersey Medical School, 225 Warren Street, Newark, NJ 07103, USA
    Science 317:526-9. 2007
    ..Little is known about how these variations (noise) in natural systems affect such transitions. In Bacillus subtilis, noise in ComK, the protein that regulates competence for DNA uptake, is thought to cause cells to ..
  99. pmc Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation
    Onuma Chumsakul
    Graduate School of Information Science, Nara Institute of Science and Technology, Takayama, Ikoma, Nara, Japan
    Nucleic Acids Res 39:414-28. 2011
    AbrB is a global transcriptional regulator of Bacillus subtilis that represses the expression of many genes during exponential growth. Here, we demonstrate that AbrB and its homolog Abh bind to hundreds of sites throughout the entire B...
  100. ncbi Management of oxidative stress in Bacillus
    Peter Zuber
    Department of Science and Engineering, School of Medicine, Oregon Health and Science University, Beaverton, Oregon 97006, USA
    Annu Rev Microbiol 63:575-97. 2009
    The spore-forming bacterium and model prokaryotic genetic system, Bacillus subtilis, is extremely useful in the study of oxidative stress management through proteomic and genome-wide transcriptomic analyses, as well as through detailed ..
  101. ncbi Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation
    Miho Hayashi
    Graduate School of Information Science, Nara Institute of Science and Technology, Takayama, Ikoma, Japan
    FEMS Microbiol Lett 247:73-9. 2005
    It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation...

Research Grants68

  1. 4th ASM Conference on Prokaryotic Cell Biology and Development
    Susan S Golden; Fiscal Year: 2012
    ..studied systems: Escherichia coli (cell division, cytoskeletal structures, cell wall biosynthesis), Bacillus subtilis (cell cycle regulation, cytoskeletal structures, development, biofilm formation, cell wall biosynthesis), ..
  2. Transcriptional Control by the Global Regulator Spx
    PETER A ZUBER; Fiscal Year: 2013
    ..Spx is a transcriptional regulator that we discovered in the spore-forming bacterium, Bacillus subtilis, but is also found in Staphylococcus aureus, Streptococcus pneumoniae, Listeria monocytogenes, and Bacillus ..
  3. Cellular functions of the essential PcrA helicase in Staphylococcus aureus
    Saleem A Khan; Fiscal Year: 2013
    ..is related to the Escherichia coli UvrD and Rep helicases and is known to be essential for UV DNA repair in Bacillus subtilis. In B...
  4. Novel Bacillus subtilis based spore vaccine to tuberculosis
    Subramanian Dhandayuthapani; Fiscal Year: 2013
    ..Alternate strategies are required for better use of this vaccine. We plan to engineer recombinant Bacillus subtilis spores that express Ag85B and ESAT-6, to be used as a particulate vaccine to TB...
  5. Problems in Membrane Protein Crystallography: Hetero-Oligomeric Cytochrome b6f
    William A Cramer; Fiscal Year: 2012
    ..by site-directed mutagenesis in Nostoc and, through structure-function analysis, in firmicutes such as Bacillus subtilis that are phylogenetically close to cyanobacteria...
  6. Immune-based detection of rifampicin-resistance in HIV/TB
    Gregory P Bisson; Fiscal Year: 2013
    ..activation by rifampicin-resistant, rpoB-mutant Mtb is consistent with data from model organisms, including Bacillus subtilis and Streptomyces lividans, in that rpoB mutation can dramatically activate dormant gene networks not ..
  7. pH Regulation in Bacillus subtilis (AREA R15)
    JOAN SLONCZEWSKI; Fiscal Year: 2009
    pH Regulation in Bacillus subtilis. Acid and base stress are important for bacteria to survive in soil and aquatic environments, where pH varies drastically, as well as in the human host where pH change contributes to virulence...
  8. Role of the Spx Regulator in Streptococcus mutans
    JOSE A LEMOS; Fiscal Year: 2013
    ..mutans, including biofilm formation, cell viability and acid tolerance. In Bacillus subtilis, the global regulator Spx is a substrate of ClpXP, and accumulation of Spx has been correlated to phenotypes ..
  9. Investigating the role of Bacillithiol in metal ion homeostasis and the reactive
    Pete Chandrangsu; Fiscal Year: 2013
    ..The LMW thiol in the well-studied bacterium, Bacillus subtilis, has only recently been identified as BSH and its functions are poorly understood...
  10. REGULATION OF GLUTAMATE SYNTHESIS IN BACILLUS SUBTILIS
    ABRAHAM LINCOLN SONENSHEIN; Fiscal Year: 2011
    ..This project seeks to understand in detail the molecular mechanisms that regulate these genes in Bacillus subtilis, a well studied model Gram-positive bacterium, and Listeria monocytogenes, a model intracellular pathogen ..
  11. MOLECULAR GENETICS OF BACILLUS SUBTILIS RNA POLYMERASE
    Chester W Price; Fiscal Year: 2013
    ..Among Bacillus subtilis and related Gram positive pathogens, this response is governed by the ?B transcription factor...
  12. Genetic Approaches to Lipid Metabolism
    John E Cronan; Fiscal Year: 2013
    ..In the case of lipoic acid synthesis Bacillus subtilis requires four proteins to synthesize this cofactor whereas E. coli required only two proteins...
  13. Structure and Function of S-adenosyl-L-methionine Riboswitches
    Ailong Ke; Fiscal Year: 2013
    ..Riboswitches are especially prevalent in Gram-positive bacteria, exemplified by Bacillus subtilis as a model organism, but are also found to control essential genes in important pathogens such as Bacillus ..
  14. Global analysis of mRNA decay in Bacillus subtilis
    David H Bechhofer; Fiscal Year: 2013
    ..Our laboratory seeks to understand the process of messenger RNA decay in the model Gram-positive bacterium, Bacillus subtilis. While much is known about the mechanisms and regulation of transcription of DNA into mRNA and translation ..
  15. REGULATION OF METHIONINE METABOLISM IN BACILLUS SUBTILIS
    Tina M Henkin; Fiscal Year: 2013
    ..involves analysis of the interplay between riboswitch elements and other regulatory mechanisms, using the Bacillus subtilis metK gene, encoding SAM synthetase, as an example...
  16. Metal Ion Homeostasis in Bacillus subtilis
    John D Helmann; Fiscal Year: 2012
    This proposal investigates the responses of the model Gram positive bacterium Bacillus subtilis to metal ion starvation. When cells are starved for essential metals, such as iron or zinc, they express complex adaptive responses...
  17. Peptidoglycan synthesis during sporulation
    Jonathan Dworkin; Fiscal Year: 2012
    ..to investigate translocation during the non-essential process of sporulation in the Gram-positive bacterium Bacillus subtilis. The endospore contains a thick layer of peptidoglycan, the spore cortex, which is largely responsible for ..
  18. Structural basis of tetracycline resistance by efflux pump TetL
    DANENG WANG; Fiscal Year: 2013
    ..The efflux pump TetL from Bacillus subtilis, with 14 transmembrane 1-helices, is a member of the family of antibiotic resistance efflux proteins (Tet) ..
  19. CHEMOTACTIC SENSORY TRANSDUCTION IN BACILLUS SUBTILIS
    George W Ordal; Fiscal Year: 2012
    ..years of NIH funding has been to understand the mechanism of chemotaxis in the Gram-positive bacterium Bacillus subtilis. During the past decade, the realization has emerged that the B...
  20. Exposporium determinants of Bacillus anthracis
    George C Stewart; Fiscal Year: 2013
    ..of what we know regarding the sporulation process was learned through biochemical and genetic studies of Bacillus subtilis, a model bacterium whose spores lack an exosporium. We propose to study exosporium composition in B...
  21. REGULATION OF NITROGEN METABOLISM IN BACILLUS SUBTILIS
    Susan H Fisher; Fiscal Year: 2012
    Nitrogen metabolism in the low G+C Gram-positive bacterium Bacillus subtilis is controlled by a novel regulatory system where the enzyme glutamine synthetase is required for both glutamine synthesis and the direct control of the ..
  22. REGULATION OF BACILLUS SUBTILIS TRNA SYNTHETASE GENES
    Tina M Henkin; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Many genes involved in amino acid metabolism in Bacillus subtilis and related organisms are regulated by the T box mechanism, a novel transcription termination control system...
  23. Systematic Phenotypic Analysis of the Gram-positive Envelope
    Jason M Peters; Fiscal Year: 2013
    ..However, the envelope of Gram-positive bacteria, such as Bacillus subtilis, is fundamentally different from that of E. coli. The B...
  24. Integration of metabolism and virulence in Gram-positive bacteria
    ABRAHAM LINCOLN SONENSHEIN; Fiscal Year: 2013
    ..A major goal of the proposed project is to determine where each CodY-regulated gene in the model organism, Bacillus subtilis, falls within this spectrum, to couple the positioning of genes to the intracellular pools of the CodY ..
  25. Cell-cell signaling, gene expression, and horizontal gene transfer in Bacillus
    Alan D Grossman; Fiscal Year: 2013
    ..on cell-cell signaling and the regulatory mechanisms controlling a mobile genetic element in the bacterium Bacillus subtilis. Mobile genetic elements include phages, conjugative plasmids, and conjugative transposons, also known as ..
  26. Intramembrane-Cleaving metalloproteases of Bacillus subtilis
    LEE R KROOS; Fiscal Year: 2013
    ..To fill this knowledge gap, most of the project focuses on SpoIVFB, which cleaves Pro-?K during Bacillus subtilis sporulation. The cleavage reaction has been reconstituted in vitro and requires ATP...
  27. Spores as a protein display platform for directed evolution of membrane proteins
    Edgardo T Farinas; Fiscal Year: 2012
    ..b>Bacillus subtilis spores will be developed as a protein display platform for direct or laboratory evolution...
  28. Regulation of pluripotent differentiation by gene circuit interactions.
    GUROL MEHMET SUEL; Fiscal Year: 2013
    ..We will study pluripotent differentiation in Bacillus subtilis, a simple, well characterized and experimentally accessible system as a model for genetic control of cell ..
  29. Dynamics of Antimicrobial Peptide Interactions with Bacterial Membranes
    James C Weisshaar; Fiscal Year: 2013
    ..coli and the Gram positive Bacillus subtilis. These include LL-37, cecropin A, magainin-2, melittin, alamethicin, indolicidin, bactenecin-5, 1-defensin, ..
  30. GENE EXPRESSION DURING SPORULATION
    Patrick J Piggot; Fiscal Year: 2012
    This proposal focuses on how spores are formed by Bacillus subtilis. B. subtilis has become a paradigm for the study of spore formation, and of cell differentiation in prokaryotes, because of the ease of its genetic manipulation...
  31. Essential Autolysin Activity in Gram-positive Bacteria
    Hendrik Szurmant; Fiscal Year: 2013
    ..knowledge we propose here to discover the concerted interplay between the many players that are involved in Bacillus subtilis cell wall homeostasis as a model for Gram-positive cell wall maintenance...
  32. DNA segregation during Bacillus growth and development
    JOSEPH A POGLIANO; Fiscal Year: 2013
    ..also identified several families of divergent actin-like proteins involved in plasmid DNA segregation in Bacillus subtilis. AlfA of plasmid pLS32 and Alp7A both assemble polymers, but they appear to segregate plasmids by different ..
  33. Study of the interplay of motility mechanisms during swaming of Myxococcus xanthu
    Mark Alber; Fiscal Year: 2013
    ..Many species of bacteria swarm, including species found in diverse soil and water environments such as Bacillus subtilis, Serratia liquefaciens, Proteus mirabilis, Pseudomonas aeruginosa, and Myxococcus xanthus...
  34. A Feeding Tube Model for Bacterial Cell-Cell Communication
    AMY HITCHCOCK CAMP; Fiscal Year: 2012
    ..metabolite exchange drives developmental gene expres- sion during spore formation by the model bacterium Bacillus subtilis. More specifically, the central hypothesis of this proposal is that the developing spore (the forespore) is ..
  35. Regulation of Magnesium Homeostasis in Bacillus subtilis
    Wade Winkler; Fiscal Year: 2011
    ..We have demonstrated that this RNA functions as a genetic sensor for magnesium in Bacillus subtilis, the model system for Gram-positive bacteria, and controls expression of one of the three magnesium ..
  36. The role of pseudopilins during transformation in Bacillus subtilis
    JESSICA MARIE MANN; Fiscal Year: 2010
    ..The machinery that mediates transformation in Bacillus subtilis is highly conserved among competent microbes, both gram-positive and negative. Also, B...
  37. Assembly dynamics and cellular function of Actin-like proteins in bacteria
    R Dyche Mullins; Fiscal Year: 2013
    ..In this project we focus on a gram positive actin-like protein, called AlfA, which stabilizes plasmids in Bacillus subtilis during both vegetative growth and sporulation...
  38. Cell Cycle and sporulation in Bacillis subtillis
    Alan D Grossman; Fiscal Year: 2013
    ..focuses on several aspects of chromosome dynamics and gene expression in the Gram- positive bacterium Bacillus subtilis. We will use a variety of approaches and methodologies, both in vivo and in vitro, to characterize genes ..
  39. Regulation of the tryptophan genes in Bacillus
    Paul Gollnick; Fiscal Year: 2009
    ..protein, an RNA binding protein that regulates transcription attenuation of the tryptophan (trp) genes in Bacillus subtilis and related bacteria...
  40. B. subtilis Reduces Intestinal Inflammation via TLR Signaling and B1 Lymphocytes
    SARA ELIZABETH JONES; Fiscal Year: 2012
    ..in intestinal health, we use an animal model in which mice are colonized with the commensal microbe Bacillus subtilis prior to infection with the attaching and effacing (A/E) pathogen, Citrobacter rodentium...
  41. Stochastic Pulsing in Microbial Regulation and Differentiation
    Michael B Elowitz; Fiscal Year: 2013
    ..We will address these questions in Bacillus subtilis, because it contains multiple circuits that use pulsing, often simultaneously, to regulate key stress ..
  42. A Switch in Gene Expression During Bacterial Development
    AMY HITCHCOCK CAMP; Fiscal Year: 2012
    ..switch from early ([unreadable]F-directed) to late ([unreadable]G-directed) transcription in the developing Bacillus subtilis spore...
  43. Transmission Electron Microscope in the Academic Health Center
    Wei Zhang; Fiscal Year: 2010
    ..will use the TEM to 1) determine the structure and molecular mechanisms of assembly of bacteriophage X29 of Bacillus subtilis, a model for assembly of animal viruses and a means to identify targets for antiviral agents;2) determine ..
  44. Discovery of Inhibitors of DNA Replication in Gram-Positive Bacteria
    H Garry Dallmann; Fiscal Year: 2010
    Using a model Gram (+) organism, Bacillus subtilis, we propose to develop and run robust high throughput screening assays as well as appropriate specificity assays and counterscreens to enable the discovery of small molecule inhibitors ..
  45. A multidisciplinary approach to elucidating gene function in a model Gram-positiv
    David Z Rudner; Fiscal Year: 2010
    ..b>Bacillus subtilis is the premier model organism for studies of Gram-positive bacteria because it offers powerful classical and ..
  46. Mechanistic studies on peptidoglycan tailoring that mediate biofilm stability
    MATTHEW DENNIS LEBAR; Fiscal Year: 2013
    ..proteins (PBPs) are responsible for non-canonical D-amino acid incorporation into the peptidoglycan of Bacillus subtilis and, once incorporated, play a role in biofilm stability...
  47. Chromosome Dynamics in Bacillus subtilis
    David Z Rudner; Fiscal Year: 2012
    ..We propose to study the organization and segregation of the chromosome in the model gram-positive bacterium Bacillus subtilis. The ease with which genetic, biochemical and cytological analysis can be carried out in this organism makes ..
  48. ROLE OF RNA POLYMERASE IN BACTERIAL DIFFERENTIATION
    RICHARD MARC LOSICK; Fiscal Year: 2013
    ..that turn on, and distinguish between, developmental programs of spore formation and matrix production by Bacillus subtilis. Spore formation has been traditionally viewed as a behavior of free-living cells, but we now understand ..
  49. ECF Sigma Factors and Cell Envelope Stress in Bacillus subtilis
    John D Helmann; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): This proposal investigates cell envelope stress responses in Bacillus subtilis, a model organism for the Gram positive bacteria...
  50. Control of elongation of DNA replication
    Jue D Wang; Fiscal Year: 2012
    ..to identify a novel nutrient-responsive regulation of replication elongation in the Gram-positive bacterium Bacillus subtilis. We propose that this regulation keeps replication elongation under metabolic control to prevent disruption ..
  51. Functional analysis of a potassium sensing kinase in Bacillus subtilis
    ERIN ANN GONTANG; Fiscal Year: 2011
    ..Recently, a novel mechanism for triggering multicellularity in Bacillus subtilis was described and surprisingly, the signaling mechanism involves a histidine kinase activated by potassium ..
  52. REGULATION OF GENETIC COMPETENCE IN BACILLUS SUBTILIS
    DAVID A DUBNAU; Fiscal Year: 2013
    ..Competence regulation in Bacillus subtilis involves a complex signal transduction network that governs the expression of a large regulon...
  53. Screening for Inhibitors of DNA Replication in Gram-Positive Bacteria
    H Garry Dallmann; Fiscal Year: 2010
    Using a model Gram (+) organism, Bacillus subtilis, we propose to transfer to the MLPCN, a robust high throughput screening assay targeting the DNA Polymerase III holoenzyme...
  54. A systems level analysis of spore coat assembly in Bacillus subtilis
    Patrick Eichenberger; Fiscal Year: 2012
    ..to characterize the protein interaction networks that govern spore coat assembly in the model organism Bacillus subtilis. Specifically, we will use a library of fluorescent protein fusions to all of the coat proteins in B...
  55. The chemical and genetic basis of interspecies interactions
    KIT J POGLIANO; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Bacillus subtilis produces a wide array of extracellular metabolites that can inhibit the growth of bacteria and fungi or modify their behavior to attenuate the production of antibacterial products ..
  56. The mechanism of bistable motility gene expression in Bacillus subtilis.
    Daniel B Kearns; Fiscal Year: 2013
    ..Here we explore the unique features of the bistable expression of motility genes in Bacillus subtilis that generates mixed populations of single motile cells and non-motile chains through an epigenetic ..
  57. Effectors and regulators of end-dependent mRNA degradation in bacteria
    MONICA PING TING HUI; Fiscal Year: 2013
    ..is to further our understanding of 5'end-dependent RNA decay in bacteria, using Escherichia coli and Bacillus subtilis as model organisms...
  58. Analysis of Required Proteins for Engulfment during Bacillus subtilis Sporulation
    RACHELLE MARIE TRIAL; Fiscal Year: 2011
    The phagocytosis-like process of engulfment, a critical step in the sporulation pathway of Bacillus subtilis, provides an ideal system to study how bacterial cells move macromolecules, localize proteins and catalyze membrane fission...
  59. Protection from enteric pathogens by beneficial microbes
    Katherine L Knight; Fiscal Year: 2013
    ..We recently found that a single oral dose of commensal Bacillus subtilis or Bacillus formis spores can protect mice from disease caused by the A/E pathogen, Citrobacter rodentium...
  60. The DNA Packaging Motor of Bacteriophage Phi29
    MARC MORAIS; Fiscal Year: 2013
    The mechanism of DNA packaging for double-stranded DNA viruses will be studied in the Bacillus subtilis bacteriophage [unreadable]29, the most efficient in vitro viral packaging system known...
  61. MOLECULAR GENETICS OF BIOFILM FORMATION
    ROBERTO G KOLTER; Fiscal Year: 2013
    ..our continued efforts to critically test aspects of this central hypothesis using the sporulating bacterium Bacillus subtilis. Through a combination of genetic and biochemical approaches we have identified a key signal transduction ..
  62. Structural Basis for Sporulation in Bacillus
    Seth A Darst; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Sporulation in Bacillus subtilis has been studied extensively as a model for cellular development...
  63. INITIATION OF MRNA DECAY IN BACILLUS SUBTILIS
    David H Bechhofer; Fiscal Year: 2010
    ..Our laboratory seeks to understand the control of messenger RNA decay in the gram-positive bacterium, Bacillus subtilis. While much is known about the mechanism and regulation of mRNA synthesis (transcription) and translation of ..
  64. Systems Biology of Sporulation in Bacillus Subtilis
    Jun Liu; Fiscal Year: 2009
    ..bear on understanding developmental gene control in the experimental system of sporulation in the bacterium Bacillus subtilis. This is an ideal system for such an interdisciplinary approach because sporulation is a relatively ..
  65. ACETOIN PRODUCTION BY BACILLUS SUBTILIS
    STANLEY ZAHLER; Fiscal Year: 1992
    ..We will use as a model system the production of acetoin (2-oxo, 3-hydroxy-butane) by Bacillus subtilis. This compound is excreted by stationary-phase cells in large quantities...
  66. THE PENICILLIN-BINDING PROTEINS OF BACILLUS SUBTILIS
    CHRISTINE BUCHANAN; Fiscal Year: 1993
    The purpose of this project is to examine and evaluate the penicillin-binding proteins (PBPs) of Bacillus subtilis under a variety of physiological conditions...
  67. MOLECULAR GENETICS AND MECHANISM OF PROTEIN ANTIBIOTICS
    J Hansen; Fiscal Year: 2001
    Certain strains of Bacillus subtilis produce subtilin, which is a broad- spectrum ribosomally-synthesized antibiotic peptide that contains many unusual amino acids (dehydroalanine, dehydrobutyrine, lanthionine, beta- methyllanthionine) ..
  68. Mechanism of Peptide Signaling in Bacillus Subtilis
    BETH LAZAZZERA; Fiscal Year: 2006
    ..mechanism of peptide signaling in Gram-positive bacteria using Competence and Sporulation Factor (CSF) of Bacillus subtilis as a model system. CSF is a member of an emerging class of signaling peptides that function intracellularly...