spheroplasts

Summary

Summary: Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.

Top Publications

  1. pmc Copper-zinc superoxide dismutase of Caulobacter crescentus: cloning, sequencing, and mapping of the gene and periplasmic location of the enzyme
    H M Steinman
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461
    J Bacteriol 172:2901-10. 1990
  2. pmc A novel periplasmic carrier protein involved in the sorting and transport of Escherichia coli lipoproteins destined for the outer membrane
    S Matsuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    EMBO J 14:3365-72. 1995
  3. pmc Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS
    Takeshi Nomura
    Molecular Cardiology and Biophysics Divison Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales 2010, Australia
    Proc Natl Acad Sci U S A 109:8770-5. 2012
  4. pmc De novo growth zone formation from fission yeast spheroplasts
    Felice D Kelly
    The Rockefeller University, New York, New York, United States of America
    PLoS ONE 6:e27977. 2011
  5. pmc HIV type 1 Gag virus-like particle budding from spheroplasts of Saccharomyces cerevisiae
    Sayuri Sakuragi
    Kitasato Institute and Kitasato University, Shirokane 5 9 1, Minato ku, Tokyo 108 8641, Japan
    Proc Natl Acad Sci U S A 99:7956-61. 2002
  6. ncbi A large-conductance mechanosensitive channel in E. coli encoded by mscL alone
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706
    Nature 368:265-8. 1994
  7. pmc Effects of GsMTx4 on bacterial mechanosensitive channels in inside-out patches from giant spheroplasts
    Kishore Kamaraju
    Department of Biology, University of Maryland, College Park, Maryland, USA
    Biophys J 99:2870-8. 2010
  8. ncbi Mechanosensitive channel functions to alleviate the cell lysis of marine bacterium, Vibrio alginolyticus, by osmotic downshock
    Y Nakamaru
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 444:170-2. 1999
  9. ncbi Enrichment of Escherichia coli spheroplasts displaying scFv antibodies specific for antigens expressed on the human cell surface
    Jun Kang Qiu
    National Laboratory of Biomacromolecules, Center for Computational and Systems Biology, Institute of Biophysics, Chinese Academy of Sciences, No 15 Datun Road, Chaoyang District, Beijing 100101, People s Republic of China
    Appl Microbiol Biotechnol 88:1385-91. 2010
  10. pmc Novel method for preparing spheroplasts from cells with an internal cellulosic cell wall
    Alvin C M Kwok
    Department of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, People s Republic of China
    Eukaryot Cell 6:563-7. 2007

Research Grants

Detail Information

Publications210 found, 100 shown here

  1. pmc Copper-zinc superoxide dismutase of Caulobacter crescentus: cloning, sequencing, and mapping of the gene and periplasmic location of the enzyme
    H M Steinman
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461
    J Bacteriol 172:2901-10. 1990
    ..crescentus chromosome. Through analysis of spheroplasts, the two SODs of C...
  2. pmc A novel periplasmic carrier protein involved in the sorting and transport of Escherichia coli lipoproteins destined for the outer membrane
    S Matsuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    EMBO J 14:3365-72. 1995
    ..The major outer membrane lipoprotein (Lpp) expressed in spheroplasts was, however, retained in the inner membrane as a mature form...
  3. pmc Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS
    Takeshi Nomura
    Molecular Cardiology and Biophysics Divison Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales 2010, Australia
    Proc Natl Acad Sci U S A 109:8770-5. 2012
    ....
  4. pmc De novo growth zone formation from fission yeast spheroplasts
    Felice D Kelly
    The Rockefeller University, New York, New York, United States of America
    PLoS ONE 6:e27977. 2011
    ..As spheroplasts recover, new growth zones form that resemble normal growing cell tips in shape and width, and polarized growth ..
  5. pmc HIV type 1 Gag virus-like particle budding from spheroplasts of Saccharomyces cerevisiae
    Sayuri Sakuragi
    Kitasato Institute and Kitasato University, Shirokane 5 9 1, Minato ku, Tokyo 108 8641, Japan
    Proc Natl Acad Sci U S A 99:7956-61. 2002
    ..show that, after removal of the cell wall, the expression of HIV type 1 Gag protein in Saccharomyces cerevisiae spheroplasts allowed simultaneous budding of VLPs from the plasma membrane...
  6. ncbi A large-conductance mechanosensitive channel in E. coli encoded by mscL alone
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706
    Nature 368:265-8. 1994
    ..The mscL nucleotide sequence predicts a unique protein of only 136 amino acids, with a highly hydrophobic core and very different from porins or other known proteins...
  7. pmc Effects of GsMTx4 on bacterial mechanosensitive channels in inside-out patches from giant spheroplasts
    Kishore Kamaraju
    Department of Biology, University of Maryland, College Park, Maryland, USA
    Biophys J 99:2870-8. 2010
    ..the effects of cytoplasmic GsMTx4 on the two bacterial channels, MscS and MscL, in giant Escherichia coli spheroplasts. In excised inside-out patches, GsMTx4 sensitized both channels to tension by increasing the opening rate and ..
  8. ncbi Mechanosensitive channel functions to alleviate the cell lysis of marine bacterium, Vibrio alginolyticus, by osmotic downshock
    Y Nakamaru
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 444:170-2. 1999
    ..This is the first report to show a physiological role of the mechanosensitive channel with large conductance...
  9. ncbi Enrichment of Escherichia coli spheroplasts displaying scFv antibodies specific for antigens expressed on the human cell surface
    Jun Kang Qiu
    National Laboratory of Biomacromolecules, Center for Computational and Systems Biology, Institute of Biophysics, Chinese Academy of Sciences, No 15 Datun Road, Chaoyang District, Beijing 100101, People s Republic of China
    Appl Microbiol Biotechnol 88:1385-91. 2010
    ..In this study, we demonstrate that Escherichia coli spheroplasts, displaying antibodies and screened by a combination of plate-panning and flow cytometric sorting, can be used ..
  10. pmc Novel method for preparing spheroplasts from cells with an internal cellulosic cell wall
    Alvin C M Kwok
    Department of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, People s Republic of China
    Eukaryot Cell 6:563-7. 2007
    ....
  11. ncbi Concentration dependent effect of GsMTx4 on mechanosensitive channels of small conductance in E. coli spheroplasts
    Annette C Hurst
    Molecular Biophysics Laboratory, School of Biomedical Sciences, University of Queensland, St Lucia, QLD, 4072, Australia
    Eur Biophys J 38:415-25. 2009
    ..coli spheroplasts using the patch-clamp technique...
  12. pmc Two types of mechanosensitive channels in the Escherichia coli cell envelope: solubilization and functional reconstitution
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706
    Biophys J 65:177-83. 1993
    ..MSCs from Escherichia coli (strain AW740) were examined using the patch-clamp technique, either (a) in giant spheroplasts, (b) after reconstitution by fusing native membrane vesicles with asolectin liposomes, or (c) by reassembly of ..
  13. ncbi Lipopolysaccharide transport to the bacterial outer membrane in spheroplasts
    Boris Tefsen
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Biol Chem 280:4504-9. 2005
    ..Whereas newly synthesized lipoproteins could be released from spheroplasts of Escherichia coli upon addition of a periplasmic extract containing LolA, de novo synthesized LPS was not ..
  14. ncbi Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation
    P C Moe
    Laboratory of Molecular Biology and Department of Bacteriology, University of Wisconsin Madison, 53706, USA
    Mol Microbiol 28:583-92. 1998
    ..This structural conservation is providing insight into regions of this molecule that are vital to its role as a mechanosensitive channel and may have broader implications for the understanding of other mechanosensitive systems...
  15. ncbi Development of a rapid and convenient method for the quantification of HIV-1 budding
    Sayuri Sakuragi
    Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3 1, Yamadaoka, Suita City, Osaka, Japan
    Microbes Infect 8:1875-81. 2006
    ..We have previously reported that spheroplasts of Saccharomyces cerevisiae expressing HIV-1 Gag proteins from an episomal plasmid constitutively released a ..
  16. ncbi Transformation of yeast by infectious prion particles
    Chih Yen King
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    Methods 39:68-71. 2006
    ..The methodology can be used to introduce different [PSI(+)] particles to many laboratory yeast genetic backgrounds. It can be adapted for applications in other yeast prion systems as well...
  17. pmc The "dashpot" mechanism of stretch-dependent gating in MscS
    Bradley Akitake
    Department of Biology, University of Maryland, College Park, MD 20742, USA
    J Gen Physiol 125:143-54. 2005
    ..Here we characterized the gating of MscS in Escherichia coli spheroplasts in a triple mutant (mscL-, mscS-, mscK-) background...
  18. pmc Gain-of-function mutations reveal expanded intermediate states and a sequential action of two gates in MscL
    Andriy Anishkin
    Department of Biology, University of Maryland, College Park, MD 20742, USA
    J Gen Physiol 125:155-70. 2005
    ..These data strongly suggest that the bulk of conductance gain in the second subtransition (S-->O) occurs through the opening of the NH2-terminal (S1) gate and the linkers are coupling elements between the M1 and S1 gates...
  19. pmc A genetic system for direct selection of gene-positive clones during recombinational cloning in yeast
    Vladimir Noskov
    Laboratory of Biosystems and Cancer, National Cancer Institute, NIH, Building 37, Room 5032, Bethesda, MD 20892 4264, USA
    Nucleic Acids Res 30:E8. 2002
    ..This method produces positive YAC recombinants at a frequency of approximately 40%. This novel TAR cloning method provides a powerful tool for structural and functional analysis of complex genomes...
  20. ncbi Effect of high hydrostatic pressure on the bacterial mechanosensitive channel MscS
    A G Macdonald
    Department of Biomedical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, UK
    Eur Biophys J 34:434-41. 2005
    ..These observations also have implications for our understanding of the adaptation of mechanosensitive channels in deep-sea bacteria...
  21. ncbi Binding and enrichment of Escherichia coli spheroplasts expressing inner membrane tethered scFv antibodies on surface immobilized antigens
    Sang Taek Jung
    Department of Chemical Engineering, University of Texas, Austin, Texas 78712, USA
    Biotechnol Bioeng 98:39-47. 2007
    ..Conversion of the cells into spheroplasts and incubation with soluble fluorescently conjugated ligands results in the specific labeling of cells ..
  22. ncbi Mounting of Escherichia coli spheroplasts for AFM imaging
    C J Sullivan
    Genome Science and Technology, The University of Tennessee, Knoxville, TN 37932, USA
    Ultramicroscopy 105:96-102. 2005
    ..AFM images demonstrate that spheroplasts can be secured with warm gelatin applied to the mica substrate just before the addition of a spheroplast ..
  23. ncbi Preliminary characterization of chemically generated Mycobacterium avium subsp. paratuberculosis cell wall deficient forms (spheroplasts)
    Murray E Hines
    Tifton Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia, P O Box 1389, 43 Brighton Road, Tifton, GA 31793, USA
    Vet Microbiol 95:247-58. 2003
    Cell wall deficient forms (CWD, spheroplasts) genetically indistinguishable from M. avium subsp. paratuberculosis (MAP) have been isolated from patients with Crohn's disease and sarcoidosis...
  24. pmc Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity
    K Yoshimura
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biophys J 77:1960-72. 1999
    ..These results suggest a model for gating in which Gly(22) moves from a hydrophobic, and through a hydrophilic, environment upon transition from the closed to open conformation...
  25. ncbi Cell volume changes during rapid temperature shifts
    Patrick Gervais
    Laboratoire de Genie des Procedes Alimentaires et Biotechnologiques, ENSBANA, 21000 Dijon, France
    J Biotechnol 102:269-79. 2003
    ..Volume variation of yeast cells, yeast spheroplasts and human leukaemia cells was measured during the transient phase after a thermal shift...
  26. pmc A new periplasmic protein of Escherichia coli which is synthesized in spheroplasts but not in intact cells
    S Hagenmaier
    Max Planck Institut fur Biologie, Tubingen, Germany
    J Bacteriol 179:2073-6. 1997
    ..It was not detectable immunologically in intact cells but was produced abundantly in spheroplasts. It could be a stress protein specific for spheroplasting.
  27. pmc Comparative roles of the cell wall and cell membrane in limiting uptake of xenobiotic molecules by Saccharomyces cerevisiae
    Mustapha Aouida
    Laboratoire Vectorologie et Transfert de Gènes, UMR 8121 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Antimicrob Agents Chemother 47:2012-4. 2003
    Using reversible electropermeabilization of cells and spheroplasts, we show that the cell wall and plasma membrane partly account for bleomycin resistance by acting as two independent barriers...
  28. pmc A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast
    Vladimir N Noskov
    Laboratory of Biosystems and Cancer, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    BMC Genomics 4:16. 2003
    ..The technique involves homologous recombination, during yeast spheroplast transformation, between genomic DNA and a TAR vector that has short (approximately 60 bp) 5' and 3' gene targeting sequences (hooks)...
  29. ncbi Translocation of colicin from the receptor to the inner cell membrane: function of the peptidoglycan layer
    J Smarda
    Department of Biology, Faculty of Medicine, Masaryk University, 662 43 Brno, Czechia
    Folia Microbiol (Praha) 47:213-7. 2002
    Sensitivity of spheroplasts (prepared in two ways) of a colicin-sensitive strain, of colicin-resistant and of colicin-tolerant mutants and of strains immune to colicins E1 and E2 was estimated and compared...
  30. pmc Disruption of lolCDE, encoding an ATP-binding cassette transporter, is lethal for Escherichia coli and prevents release of lipoproteins from the inner membrane
    Shin ichiro Narita
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Bacteriol 184:1417-22. 2002
    ..From these results, we conclude that the LolCDE complex is an essential ABC transporter for E. coli and the sole apparatus mediating the release of outer membrane lipoproteins from the inner membrane...
  31. pmc The gaf fimbrial gene cluster of Escherichia coli expresses a full-size and a truncated soluble adhesin protein
    J Tanskanen
    Division of General Microbiology, Department of Biosciences, FIN-00014 University of Helsinki, Finland
    J Bacteriol 183:512-9. 2001
    ..Only trace amounts of truncated forms with expected molecular sizes were detected in spheroplasts. In contrast, the shorter GafD1-157 peptide was detected in spheroplasts but not in the periplasm, indicating ..
  32. pmc Topological characterization of the essential Escherichia coli cell division protein FtsN
    K Dai
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City 66160, USA
    J Bacteriol 178:1328-34. 1996
    ..It is concluded that the N-terminal, cytoplasmic, and transmembrane domains of FtsN are not required for function of the carboxy domain other than to transport it to the periplasm. FtsQ and FtsI were also analyzed...
  33. ncbi Killer-toxin-resistant kre12 mutants of Saccharomyces cerevisiae: genetic and biochemical evidence for a secondary K1 membrane receptor
    M J Schmitt
    , , D-55099 Mainz, Germany
    Arch Microbiol 164:435-43. 1995
    ..we mutagenized sensitive yeast strains and isolated killer-resistant (kre) mutants that were resistant as spheroplasts. Classical yeast genetics and successive back-crossings to sensitive wild-type strains indicated that this ..
  34. ncbi The effects of parabens on the mechanosensitive channels of E. coli
    Thom Nguyen
    School of Medicine and Pharmacology, QEII Medical Centre, The University of Western Australia, Crawley, WA 6009, Australia
    Eur Biophys J 34:389-95. 2005
    ..In summary, our study indicates that one of the previously unidentified mechanisms of action of parabens as antimicrobial agents is via an interaction with the mechanosensitive channels to upset the osmotic gradients in bacteria...
  35. ncbi Exploring transformation-associated recombination cloning for selective isolation of genomic regions
    Natalay Kouprina
    Laboratory of Biosystems and Cancer, National Cancer Institute, Bethesda, MD, USA
    Methods Mol Biol 255:69-89. 2004
  36. ncbi DsbB elicits a red-shift of bound ubiquinone during the catalysis of DsbA oxidation
    Kenji Inaba
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    J Biol Chem 279:6761-8. 2004
    ..We propose that the Cys44-induced anomaly in ubiquinone represents its activated state, which drives the DsbB-mediated electron transfer...
  37. pmc Gating the bacterial mechanosensitive channel MscL invivo
    Ann Finney Batiza
    Laboratory of Molecular Biology, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 99:5643-8. 2002
    ..These observations show that MscL opening is triggered by a specific downshock, even in the presence of YggB, that YggB buffers MscL gating in vivo, and that residue 19 becomes exposed upon channel opening...
  38. pmc Dislocation of membrane proteins in FtsH-mediated proteolysis
    A Kihara
    Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
    EMBO J 18:2970-81. 1999
    ..We propose that FtsH dislocates the extracytoplasmic domain of a substrate, probably using its ATPase activity...
  39. ncbi A new group of antifungal and antibacterial lipopeptides derived from non-membrane active peptides conjugated to palmitic acid
    Dorit Avrahami
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 279:12277-85. 2004
    ....
  40. pmc Defining the physical gate of a mechanosensitive channel, MscL, by engineering metal-binding sites
    Irene Iscla
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    Biophys J 87:3172-80. 2004
    ....
  41. ncbi Different action of killer toxins K1 and K2 on the plasma membrane and the cell wall of Saccharomyces cerevisiae
    Drahomíra Novotná
    Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 128 44, Prague 2, Czech Republic
    FEMS Yeast Res 4:803-13. 2004
    ..to toxin K2) showed that the phenotype is complemented by the KRE2 gene not only in intact cells but also in spheroplasts, and resistance to K1 thus resides very probably in the plasma membrane...
  42. ncbi A spheroplast rate assay for determination of cell wall integrity in yeast
    R Ovalle
    Department of Biological Sciences, Hunter College, City University of New York, New York 10021, USA
    Yeast 14:1159-66. 1998
    The rate of formation of spheroplasts of yeast can be used as an assay to study the structural integrity of cell walls...
  43. ncbi Molecular and functional study of AQY1 from Saccharomyces cerevisiae: role of the C-terminal domain
    V Laizé
    Departement de Biologie Cellulaire et Moleculaire, Service de Biologie Cellulaire, CEA Saclay, Gif sur Yvette Cedex, F 91 191, France
    Biochem Biophys Res Commun 257:139-44. 1999
    ..Pf measurements in yeast spheroplasts confirmed the presence of functional water channels in Sigma1278b and a pharmacological study indicated that ..
  44. pmc Specific cell wall proteins confer resistance to nisin upon yeast cells
    S K Dielbandhoesing
    Unilever Research Laboratorium Vlaardingen, 3133 AT Vlaardingen, The Netherlands
    Appl Environ Microbiol 64:4047-52. 1998
    ..We concluded that Cwp2p plays a prominent role in protection of cells against antimicrobial peptides, such as nisin, and that Cwp1p and Cwp2p play a key role in the formation of a normal cell wall...
  45. ncbi Decrease of H2O2 plasma membrane permeability during adaptation to H2O2 in Saccharomyces cerevisiae
    Miguel R Branco
    Grupo de Bioquímica dos Oxidantes e Antioxidantes, Centro de Quimica e Bioquimica, Universidade de Lisboa, Portugal
    J Biol Chem 279:6501-6. 2004
    ..The consequences of the results to cellular redox compartmentalization and to the origin and evolution of the eukaryotic cell are discussed...
  46. ncbi A molecular target for viral killer toxin: TOK1 potassium channels
    A Ahmed
    Department of Pediatrics, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Cell 99:283-91. 1999
    ..laevis oocytes...
  47. ncbi Visualisation of the mechanosensitive channel of large conductance in bacteria using confocal microscopy
    Christel Norman
    School of Medicine and Pharmacology, QEII Medical Centre, The University of Western Australia, Crawley, WA 6009, Australia
    Eur Biophys J 34:396-402. 2005
    ..This method could prove an interesting, non-invasive, tool to study the localisation and the regulation of expression of MscL in bacteria...
  48. pmc Involvement of inner and outer membrane components in the transport of iron and in colicin B action in Escherichia coli
    P Wookey
    J Bacteriol 133:661-6. 1978
    b>Spheroplasts of Escherichia coli mutants were used to investigate the roles of the inner and outer membranes in the transport of iron...
  49. ncbi Mutations in the MotA protein of Escherichia coli reveal domains critical for proton conduction
    D F Blair
    Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138
    J Mol Biol 221:1433-42. 1991
    ..The clustering of the mutations provides independent support for the suggestion that MotA is a transmembrane proton channel and places significant constraints on models for the molecular mechanism of ion conduction...
  50. pmc Molecular structure of the cell wall receptor for killer toxin KT28 in Saccharomyces cerevisiae
    M Schmitt
    , , Federal Republic of Germany
    J Bacteriol 170:2192-6. 1988
    ..At least two mannose residues have to be present in the side chains of the outer chain of the cell wall mannan, whereas the phosphodiester-linked mannose group is not essential for binding and the subsequent action of killer toxin KT28...
  51. ncbi K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane
    D Bossemeyer
    Fachgebiet Mikrobiologie, Universitat Osnabruck, Federal Republic of Germany
    J Biol Chem 264:16403-10. 1989
    ..These results support the model, previously based solely on genetic data, that Trk is a multisubunit complex and implicates the products of the other trk genes in the normal binding of TrkA to the complex in the cytoplasmic membrane...
  52. ncbi Targeting the expression of functional murine CMP-sialic acid transporter to the E. coli inner membrane
    Andrea Maggioni
    Institute for Glycomics, Gold Coast Campus Griffith University, Queensland 4222, Australia
    Biochem Biophys Res Commun 362:779-84. 2007
    ..coli and mixed phosphatidylcholine-E. coli inner membrane proteoliposomes incorporating recombinant CMP-sialic acid transporter to accumulate CMP-sialic acid in vitro...
  53. ncbi Topology of the yeast fatty acid transport protein Fat1p: mechanistic implications for functional domains on the cytosolic surface of the plasma membrane
    Thomas Obermeyer
    Center for Metabolic Disease, Ordway Research Institute, Albany Medical College, Albany, NY 12208, USA
    J Lipid Res 48:2354-64. 2007
    ..This topological orientation is consistent with the mechanistic roles of both Fat1p and Faa1p or Faa4p in the coupled transport/activation of exogenous fatty acids by vectorial acylation...
  54. ncbi Membrane topology inversion of SecG detected by labeling with a membrane-impermeable sulfhydryl reagent that causes a close association of SecG with SecA
    Shushi Nagamori
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Biochem 132:629-34. 2002
    ..Treatment of spheroplasts with AMS revealed that a cysteine residue in the cytoplasmic region of SecG could be labeled from the periplasm ..
  55. ncbi Application of fluorescence resonance energy transfer to examine EnvZ/OmpR interactions
    S Thomas King
    Department of Microbiology and Immunology, University of Immunology, University of Illinois Chicago, Chicago, Illinois, USA
    Methods Enzymol 422:352-60. 2007
    ..b>Spheroplasts were prepared and lysed in microtiter plates containing purified, fluorescent-labeled OmpR protein...
  56. ncbi Membrane topology of the MotA protein of Escherichia coli
    J Zhou
    Department of Biology, University of Utah, Salt Lake City 84112, USA
    J Mol Biol 251:237-42. 1995
    ..These experiments establish that the main features of the suggested model for MotA topology are correct, furnishing a basis for more detailed structure-function studies of the MotA/MotB proton channel...
  57. ncbi Sequence and topology of the CorA magnesium transport systems of Salmonella typhimurium and Escherichia coli. Identification of a new class of transport protein
    R L Smith
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965
    J Biol Chem 268:14071-80. 1993
    ..Its lack of homology to known proteins and its topology indicate that the CorA Mg2+ transporter represents a new class of membrane transport system...
  58. ncbi Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes
    Gerd P Bienert
    Department of Agricultural Sciences, Faculty of Life Science, Copenhagen University, DK 1871 Frederiksberg C, Denmark
    J Biol Chem 282:1183-92. 2007
    ..Our results present the first molecular genetic evidence for the diffusion of H2O2 through specific members of the aquaporin family...
  59. pmc Lipid-protein interaction of the MscS mechanosensitive channel examined by scanning mutagenesis
    Takeshi Nomura
    ICORP SORST, Cell Mechanosensing, Japan Science and Technology Agency, Nagoya, Japan
    Biophys J 91:2874-81. 2006
    ..This observation indicates that the lipid-protein interaction at the ends of both helices (TM1 and TM2) is essential to MscS function...
  60. ncbi Effects of the bacteriocin PsVP-10 produced by Pseudomonas sp. on sensitive bacterial strains
    Carlos Padilla
    Laboratory of Research in Microbiology and Institute of Biological Sciences and Biotechnology, Universidad de Talca, Chile
    Rev Latinoam Microbiol 44:19-23. 2002
    ..Assays performed with spheroplasts obtained from sensitive bacterial species and their resistant mutants show a rapid lethal effect of the ..
  61. pmc Function of the ypt2 gene in the exocytic pathway of Schizosaccharomyces pombe
    M W Craighead
    Imperial Cancer Research Fund, London, England
    Mol Biol Cell 4:1069-76. 1993
    ..Thus the ypt2 protein, and by inference rab8, appear to function in the last stage of the secretory pathway...
  62. pmc Membrane topologies of the TolQ and TolR proteins of Escherichia coli: inactivation of TolQ by a missense mutation in the proposed first transmembrane segment
    K Kampfenkel
    Universitat Tubingen, Germany
    J Bacteriol 175:4485-91. 1993
    ..in the cytoplasmic membrane was determined by cleavage with aminopeptidase K, proteinase K, and trypsin in spheroplasts and cell lysates...
  63. ncbi Role of Nitrosomonas europaea NitABC iron transporter in the uptake of Fe3+-siderophore complexes
    Neeraja Vajrala
    Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA
    Arch Microbiol 192:899-908. 2010
    ..b>Spheroplasts prepared from N...
  64. ncbi Bioenergetic responses of Synechocystis 6803 fatty acid desaturase mutants at low temperatures
    R A Dilley
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Bioenerg Biomembr 33:135-41. 2001
    ..b>Spheroplasts (permeabilized cells) were prepared by lysozyme digestion of the cell wall followed by gentle osmotic shock...
  65. pmc Identification and initial topological analysis of the Rickettsia prowazekii ATP/ADP translocase
    G V Plano
    Department of Microbiology and Immunology, University of South Alabama, College of Medicine, Mobile 36688
    J Bacteriol 173:3389-96. 1991
    ..coli relative to right-side-out spheroplasts, thus indicating that the carboxyl terminus of the carrier is located on the cytoplasmic side of the bacterial ..
  66. ncbi Continuous administration of PBP-2- and PBP-3-specific beta-lactams causes higher cytokine responses in murine Pseudomonas aeruginosa and Escherichia coli sepsis
    Jacqueline Buijs
    Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands
    J Antimicrob Chemother 59:926-33. 2007
    ..PBP-2-specific antibiotics induce spheroplasts, again associated with low endotoxin release...
  67. pmc Escherichia coli produces a cytoplasmic alpha-amylase, AmyA
    M Raha
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511
    J Bacteriol 174:6644-52. 1992
    ..The protein was retained by spheroplasts rather than being released with the periplasmic fraction; cells transformed with plasmids containing the gene ..
  68. ncbi Growth, photosynthesis, active oxygen species and antioxidants responses of paddy field cyanobacterium Plectonema boryanum to endosulfan stress
    Sheo Mohan Prasad
    Ranjan Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, India
    J Gen Appl Microbiol 51:115-23. 2005
    ..14C-fixation appeared to be more sensitive to insecticide than whole cell oxygen evolution. Spheroplasts treated with endosulfan exhibited a severe effect on PSII activity which was mainly due to blocking of the ..
  69. ncbi Complete genome sequence of Mycoreovirus-1/Cp9B21, a member of a novel genus within the family Reoviridae, isolated from the chestnut blight fungus Cryphonectria parasitica
    Nobuhiro Suzuki
    Agrivirology Laboratory, Research Institute for Bioresources, Okayama University, 2 20 1 Chu ou, Kurashiki, Okayama 710 0046, Japan
    J Gen Virol 85:3437-48. 2004
    ..A previous study showed that double-shelled particles introduced to fungal spheroplasts resulted in stably infected colonies...
  70. ncbi A Simplified and efficient method for transformation and gene tagging of Ustilago maydis using frozen cells
    Lianghui Ji
    Biomaterials and Biocatalysts Group, Temasek Life Sciences Laboratory, Research Link, National University of Singapore, Singapore 117604, Singapore
    Fungal Genet Biol 47:279-87. 2010
    ..Little improvement has been made in the method for its transformation since the PEG-mediated transfection of spheroplasts that was reported more than 20years ago...
  71. ncbi Effect of N oxyanions on anaerobic induction of nitrate reductase in subcellular fractions of Bradyrhizobium sp. (Lupinus)
    Władysław Polcyn
    Institute of Experimental Biology, A Mickiewicz University, Umultowska 89, 61 614 Poznan, Poland
    Antonie Van Leeuwenhoek 95:159-64. 2009
    ..strain USDA 3045 showed fivefold increase of the enzyme activity in spheroplasts, considered as the source of intact-membrane-bound nitrate reductase, within a 3 h time frame after nitrate ..
  72. ncbi Passive penetration of nitrate through the plasma membrane of Paracoccus denitrificans and its potentiation by the lipophilic tetraphenylphosphonium cation
    Igor Kucera
    Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ 61137 Brno, Czech Republic
    Biochim Biophys Acta 1557:119-24. 2003
    ..Anion uptake was also measured independently from passive swelling of uncoupled spheroplasts in iso-osmotic solutions of ammonium salts...
  73. ncbi Insulin signaling in the yeast Saccharomyces cerevisiae. 1. Stimulation of glucose metabolism and Snf1 kinase by human insulin
    G Muller
    Hoechst Marion Roussel Deutschland GmbH, D 65926 Frankfurt am Main, Germany
    Biochemistry 37:8683-95. 1998
    ..Under two conditions of growth limitation (glucose-grown cells during transition to stationary phase or spheroplasts during incubation in synthetic glucose medium), human insulin (10 and 1 microM, respectively) enhanced glycogen ..
  74. ncbi Integration of the colicin A pore-forming domain into the cytoplasmic membrane of Escherichia coli
    D Duche
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS, 31 Chemin Joseph Aiguier, Marseille Cedex 20, 13402, France
    J Mol Biol 285:1965-75. 1999
    ..associated with the outside of the inner membrane, and were therefore digested by proteases added to spheroplasts. In contrast, fusion proteins with a functional domain 1 were protected from proteases, suggesting as expected ..
  75. ncbi A heterologous system for assembly of retroviral gene vectors: intracellular budding in yeast?
    Oleg Tolmachov
    Section of Molecular and Cellular Medicine, Division of Biomedical Sciences, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, South Kensington, London SW7 2AZ, UK
    Med Hypotheses 67:807-9. 2006
    ..budding of retroviral vector particles inside yeast cells, with no need for obtaining and maintaining yeast spheroplasts or protoplasts, can be an efficient and economical method of mass production of these valuable gene therapy ..
  76. ncbi [Atypical cell forms overproducing extracellular substances in population of cycad cyanobionts]
    O I Baulina
    Faculty of Biology, Moscow State University, Vorob evy Gory, Moscow, 119992 Russia
    Mikrobiologiia 72:792-805. 2003
    ..roots contained two specific forms of vegetative cells with a reduced cell wall, namely, protoplasts and spheroplasts. The protoplasts and spheroplasts exhibited ultrastructural changes indicating the overproduction of two ..
  77. pmc Patch clamp and phenotypic analyses of a prokaryotic cyclic nucleotide-gated K+ channel using Escherichia coli as a host
    Mario Meng Chiang Kuo
    Structural Biology Laboratory, The Salk Institute, La Jolla, California 92037, USA
    J Biol Chem 282:24294-301. 2007
    ..coli membrane in vivo. After enlarging these cells into giant spheroplasts, macro- and microscopic MmaK currents are readily detected in excised E. coli membrane patches by a patch clamp...
  78. ncbi Mitochondrial function in the yeast form of the pathogenic fungus Paracoccidioides brasiliensis
    Vicente P Martins
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Sao Paulo, Brazil
    J Bioenerg Biomembr 40:297-305. 2008
    ..Respiration, membrane potential, and oxidative phosphorylation in mitochondria from P. brasiliensis spheroplasts were evaluated in situ, and the presence of a complete (Complex I-V) functional respiratory chain was ..
  79. ncbi Transcriptional inhibition of the operon for the spermidine uptake system by the substrate-binding protein PotD
    F Antognoni
    Faculty of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba 263 8522, Japan
    J Biol Chem 274:1942-8. 1999
    ..In cells overexpressing PotD, a large amount of PotD existed as PotD precursor in spheroplasts. Our results indicate that PotD precursor can also inhibit spermidine transport...
  80. pmc Use of phoA and lacZ fusions to study the membrane topology of ProW, a component of the osmoregulated ProU transport system of Escherichia coli
    M Haardt
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 178:5370-81. 1996
    ..The protease kallikrein digests the amino-terminal tail of a ProW-LacZ hybrid protein in spheroplasts, suggesting that the amino terminus of ProW is located on the periplasmic side of the cytoplasmic membrane...
  81. ncbi Skp, a molecular chaperone of gram-negative bacteria, is required for the formation of soluble periplasmic intermediates of outer membrane proteins
    U Schafer
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herderstrasse 7, D 79104 Freiburg, Germany
    J Biol Chem 274:24567-74. 1999
    ..Pulse labeling of OmpA in spheroplasts prepared from an skp null mutant revealed a specific requirement of Skp for the release of newly translocated ..
  82. pmc The Agrobacterium tumefaciens virB7 gene product, a proposed component of the T-complex transport apparatus, is a membrane-associated lipoprotein exposed at the periplasmic surface
    D Fernandez
    Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center at Houston, 77030, USA
    J Bacteriol 178:3156-67. 1996
    ..tumefaciens spheroplasts and remained intact upon treatment of whole cells...
  83. ncbi Iron-hydroxamate transport into Escherichia coli K12: localization of FhuD in the periplasm and of FhuB in the cytoplasmic membrane
    W Koster
    Universitat Tubingen, Federal Republic of Germany
    Mol Gen Genet 217:233-9. 1989
    ..FhuD protein appeared in two forms of 31 and 28 kDa and was released upon conversion of vegetative cells into spheroplasts, suggesting synthesis of FhuD as a precursor and export into the periplasm...
  84. pmc Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes
    A Gnirke
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63110
    EMBO J 10:1629-34. 1991
    ..The gene transfer was carried out both by lipofection using purified yeast DNA and by fusion between yeast spheroplasts and the hamster cells...
  85. pmc Reversion of Q beta RNA phage mutants by homologous RNA recombination
    K Palasingam
    Department of Biochemistry and Molecular Biology, Texas College of Osteopathic Medicine, University of North Texas, Fort Worth 76107
    J Virol 66:2435-42. 1992
    ..within their replicase genes were prepared from modified Q beta cDNAs and transfected into Escherichia coli spheroplasts containing Q beta replicase provided in trans by a resident plasmid...
  86. ncbi Antifungal activity of ibuprofen alone and in combination with fluconazole against Candida species
    C Pina-Vaz
    Department of Microbiology, Porto School of Medicine, University of Porto, Portugal
    J Med Microbiol 49:831-40. 2000
    ..accompanied by rapid and extensive leakage of intracellular K+, permeation to propidium iodide, lysis of spheroplasts and severe membrane ultrastructural alterations...
  87. ncbi [The correcting role of autonomous 3'-->5' exonucleases in mammalian multienzyme DNA polymerase complexes]
    I V Shevelev
    Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Oblast, 188300 Russia
    Mol Biol (Mosk) 36:1055-61. 2002
    ..DNA was synthesized on phage psi X174 amber3 or M13mp2 primer-templates, and used to transfect Escherichia coli spheroplasts. Frequencies were estimated for direct and reverse mutations resulting from mistakes made in the course of in ..
  88. pmc Location of the basal disk and a ringlike cytoplasmic structure, two additional structures of the flagellar apparatus of Wolinella succinogenes
    S C Schuster
    Max Planck Institut fuer Biochemie, Martinsried, Germany
    J Bacteriol 174:263-8. 1992
    ..The L-P ring complex is therefore assumed to be a bushing for the rod. Thin sections of whole bacteria and spheroplasts reveal that the disk is attached to the inner surface of the outer membrane...
  89. pmc 2,2,2-Trifluoroethanol changes the transition kinetics and subunit interactions in the small bacterial mechanosensitive channel MscS
    Bradley Akitake
    Department of Biology, University of Maryland, College Park, Maryland
    Biophys J 92:2771-84. 2007
    ..Patch-clamp analysis of giant E. coli spheroplasts expressing MscS shows that exposure to TFE in lower concentrations (0.5-5...
  90. ncbi A mitochondrial pyruvate dehydrogenase bypass in the yeast Saccharomyces cerevisiae
    S Boubekeur
    Institut de Biochimie et Genetique Cellulaires du CNRS, Universite Victor Segalen Bordeaux, 1 rue Camille Saint Saens, 33077 Bordeaux Cedex, France
    J Biol Chem 274:21044-8. 1999
    b>Spheroplasts of the yeast Saccharomyces cerevisiae oxidize pyruvate at a high respiratory rate, whereas isolated mitochondria do not unless malate is added...
  91. ncbi Polyisoprenyl phosphate specificity of UDP-GlcNAc:undecaprenyl phosphate N-acetylglucosaminyl 1-P transferase from E.coli
    J S Rush
    Department of Biochemistry, University of Kentucky College of Medicine, Lexington 40536, USA
    Glycobiology 7:315-22. 1997
    ..The enzyme remained bound to the membrane fraction when spheroplasts, formed by lysozyme-EDTA treatment, were lysed in hypotonic buffer...
  92. pmc Gating of the large mechanosensitive channel in situ: estimation of the spatial scale of the transition from channel population responses
    Chien Sung Chiang
    Department of Biology, University of Maryland College Park, Maryland 20742, USA
    Biophys J 86:2846-61. 2004
    ..conducted a systematic study of currents and dose-response curves for wild-type MscL in Escherichia coli giant spheroplasts. Using the all-point histogram method and calibrating tension against the threshold for the small ..
  93. ncbi A yeast telomere binding activity binds to two related telomere sequence motifs and is indistinguishable from RAP1
    M S Longtine
    Department of Plant Biology, University of Minnesota, St Paul 55108
    Curr Genet 16:225-39. 1989
    ..In extracts of yeast spheroplasts prepared by incubation of yeast cells with Zymolyase, an altered, proteolyzed form, of TBA (TBA-S) is present...
  94. ncbi The early interaction of the outer membrane protein phoe with the periplasmic chaperone Skp occurs at the cytoplasmic membrane
    N Harms
    Department of Molecular Microbiology, Institute of Molecular Biological Sciences, BioCentrum Amsterdam, De Boelelaan 1087, Amsterdam 1081 HV, The Netherlands
    J Biol Chem 276:18804-11. 2001
    b>Spheroplasts were used to study the early interactions of newly synthesized outer membrane protein PhoE with periplasmic proteins employing a protein cross-linking approach...
  95. ncbi Cloning and functional expression of an MscL ortholog from Rhizobium etli: characterization of a mechanosensitive channel
    Daniel Balleza
    Laboratory of Molecular Biology, University of Wisconsin, Madison, WI, 53706, USA
    J Membr Biol 234:13-27. 2010
    ..etli in E. coli to examine its activity by patch clamp in giant spheroplasts and characterized it at the single-channel level. We obtained evidence that ReMscL prevents the lysis of E...
  96. pmc Outer membrane localization of murein hydrolases: MltA, a third lipoprotein lytic transglycosylase in Escherichia coli
    J Lommatzsch
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    J Bacteriol 179:5465-70. 1997
    ..30 degrees C, the optimal temperature for enzyme activity, but not at 37 degrees C results in the formation of spheroplasts. Not only a deletion mutant in mltA, but also double mutants in mltA and one of the two other well-..
  97. ncbi Complement-mediated bacteriolysis after binding of specific antibodies to drug-resistant Pseudomonas aeruginosa: morphological changes observed by using a field emission scanning electron microscope
    Jun Tanaka
    Osaka Research Laboratory, Benesis Corporation, 3 16 89 Kashima, Yodogawa ku, Osaka, Osaka 532 8505, Japan
    J Infect Chemother 16:383-7. 2010
    ..When the bacteria were treated with meropenem, a carbapenem antibiotic, spheroplasts and bulges (spheroidization) appeared after 1-3 h...
  98. ncbi The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation
    Kirsten Börngen
    Institute of Biochemistry, University of Cologne, 50674 Cologne, Germany
    Biochim Biophys Acta 1798:2141-9. 2010
    ..coli spheroplasts MscCG showed the typical pressure dependent gating behavior of a stretch-activated channel with a current/..
  99. ncbi Purification of yeast membranes and organelles by sucrose density gradient centrifugation
    Jennifer Chang
    Department of Biological Sciences, St John s University, Queens, NY, USA
    Methods Mol Biol 457:141-9. 2008
    ..Yeast cells are converted to spheroplasts and gently lysed under conditions that preserve the integrity of organelles...
  100. ncbi Identification of hyperpolarization-activated calcium channels in apical pollen tubes of Pyrus pyrifolia
    Hai Yong Qu
    College of Horticulture, Nanjing Agricultural University, Nanjing, China
    New Phytol 174:524-36. 2007
    ..Here, we developed a protocol for successful isolation of spheroplasts from pollen tubes of Pyrus pyrifolia and identified a hyperpolarization-activated cation channel using the ..
  101. pmc Antifungal activity of local anesthetics against Candida species
    C Pina-Vaz
    Department of Microbiology, Porto School of Medicine, University of Porto, Portugal
    Infect Dis Obstet Gynecol 8:124-37. 2000
    ..To evaluate the activity of benzydamine, lidocaine, and bupivacaine, three drugs with local anesthetic activity, against Candida albicans and non-albicans strains and to clarify their mechanism of activity...

Research Grants3

  1. Characterization of OmpR Gene Regulation
    Linda J Kenney; Fiscal Year: 2010
    ..An EnvZ-GFP fusion will be employed to examine signaling and transcription in intact cells and in spheroplasts to determine the role (if any) of the outer membrane...
  2. KILLING OF PSEUDOMONAS BY A GRANULE PROTEIN FROM PMNL
    BEULAH GRAY; Fiscal Year: 1990
    ..The capacity of the fragments to kill intact P. aeruginosa cells and carbenicillin-induced spheroplasts of bacteria will be determined...
  3. Structure Analysis of Aquaporin Membrane Channels
    Mark Yeager; Fiscal Year: 2006
    ..In addition, we have developed an osmotic-shock assay in yeast spheroplasts to verify that the recombinant protein exhibits water channel activity...