methanobacterium

Summary

Summary: A genus of anaerobic, rod-shaped METHANOBACTERIACEAE. Its organisms are nonmotile and use ammonia as the sole source of nitrogen. These methanogens are found in aquatic sediments, soil, sewage, and the gastrointestinal tract of animals.

Top Publications

  1. ncbi Crystal structures of MTH1187 and its yeast ortholog YBL001c
    Xiao Tao
    Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, New York 10027, USA
    Proteins 52:478-80. 2003
  2. pmc Novel insights into K+ selectivity from high-resolution structures of an open K+ channel pore
    Sheng Ye
    Life Sciences Institute, Zhejiang University, Zhejiang, China
    Nat Struct Mol Biol 17:1019-23. 2010
  3. pmc Structural aspects and immunolocalization of the F420-reducing and non-F420-reducing hydrogenases from Methanobacterium thermoautotrophicum Marburg
    I J Braks
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, Federal Republic of Germany
    J Bacteriol 176:7677-87. 1994
  4. pmc Desulfotomaculum and Methanobacterium spp. dominate a 4- to 5-kilometer-deep fault
    Duane P Moser
    Environmental Microbiology Group, Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    Appl Environ Microbiol 71:8773-83. 2005
  5. pmc shift from acetoclastic to H2-dependent methanogenesis in a west Siberian peat bog at low pH values and isolation of an acidophilic Methanobacterium strain
    O R Kotsyurbenko
    Institut fur Mikrobiologie, Carolo Wilhelmina Technische Universität zu Braunschweig, Biozentrum, Braunschweig, Germany
    Appl Environ Microbiol 73:2344-8. 2007
  6. pmc Solution structure of ribosomal protein S28E from Methanobacterium thermoautotrophicum
    Bin Wu
    Northeast Structural Genomics Consortium, Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada
    Protein Sci 12:2831-7. 2003
  7. pmc Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum
    A Yee
    Division of Molecular and Structural Biology, Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 2M9, Canada
    Proc Natl Acad Sci U S A 97:6311-5. 2000
  8. ncbi A unique organization of the protein subunits of the DNA polymerase clamp loader in the archaeon Methanobacterium thermoautotrophicum deltaH
    Z Kelman
    Department of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 275:7327-36. 2000
  9. pmc Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase - engineering a thermostable ATP independent enzyme
    Alexander M Zhelkovsky
    New England Biolabs, Division of RNA Biology, 240 County Road, Ipswich, MA 01938 2723, USA
    BMC Mol Biol 13:24. 2012
  10. ncbi Polymorphism and double hexamer structure in the archaeal minichromosome maintenance (MCM) helicase from Methanobacterium thermoautotrophicum
    Yacob Gómez-Llorente
    Biocomputing Unit, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Darwin 3, 28049 Madrid, Spain
    J Biol Chem 280:40909-15. 2005

Research Grants

  1. Gating Mechanisms of the Prokaryotic Calcium Activated Potassium channel MthK
    DAVID JOHN POSSON; Fiscal Year: 2010
  2. Molecular Mechanism of Cation Channel Selectivity
    Youxing Jiang; Fiscal Year: 2013
  3. BIOSYNTHESIS OF RIBOFLAVIN AND RELATED COMPOUNDS
    HEINZ FLOSS; Fiscal Year: 1991
  4. Mechanism of Ligand Gating in Potassium Channels
    Youxing Jiang; Fiscal Year: 2008
  5. Mechanism of KDO 8-P and DAH 7-P Synthase
    RONALD WOODARD; Fiscal Year: 2004
  6. ENZYMOLOGY OF ACETOTROPHIC METHANOGENESIS
    JAMES FERRY; Fiscal Year: 2003
  7. Probing Microb Comm - Stable Isotope & RNA/DNA Analyses
    James Tiedje; Fiscal Year: 2004
  8. High-Accuracy Models of Proteins from Remote Homology
    Jie Liang; Fiscal Year: 2009
  9. Mechanism of A5P Isomerase
    RONALD WESLEY WOODARD; Fiscal Year: 2010

Detail Information

Publications188 found, 100 shown here

  1. ncbi Crystal structures of MTH1187 and its yeast ortholog YBL001c
    Xiao Tao
    Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, New York 10027, USA
    Proteins 52:478-80. 2003
  2. pmc Novel insights into K+ selectivity from high-resolution structures of an open K+ channel pore
    Sheng Ye
    Life Sciences Institute, Zhejiang University, Zhejiang, China
    Nat Struct Mol Biol 17:1019-23. 2010
    ..This single K+ binding at low concentration effectively blocks the permeation of Na+, providing a structural basis for the anomalous mole-fraction effect, a key property of multi-ion pores...
  3. pmc Structural aspects and immunolocalization of the F420-reducing and non-F420-reducing hydrogenases from Methanobacterium thermoautotrophicum Marburg
    I J Braks
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, Federal Republic of Germany
    J Bacteriol 176:7677-87. 1994
    ..12.99.1.) were isolated from a crude extract of Methanobacterium thermoautotrophicum Marburg...
  4. pmc Desulfotomaculum and Methanobacterium spp. dominate a 4- to 5-kilometer-deep fault
    Duane P Moser
    Environmental Microbiology Group, Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    Appl Environ Microbiol 71:8773-83. 2005
    ..and an archaeal species related to Methanobacterium spp...
  5. pmc shift from acetoclastic to H2-dependent methanogenesis in a west Siberian peat bog at low pH values and isolation of an acidophilic Methanobacterium strain
    O R Kotsyurbenko
    Institut fur Mikrobiologie, Carolo Wilhelmina Technische Universität zu Braunschweig, Biozentrum, Braunschweig, Germany
    Appl Environ Microbiol 73:2344-8. 2007
    ..8, and Methanobacteriaceae became important except for Methanomicrobiaceae and Methanosarcinaceae. An acidophilic and psychrotolerant Methanobacterium sp. was isolated using H(2)-plus-CO(2)-supplemented medium at pH 4.5.
  6. pmc Solution structure of ribosomal protein S28E from Methanobacterium thermoautotrophicum
    Bin Wu
    Northeast Structural Genomics Consortium, Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada
    Protein Sci 12:2831-7. 2003
    The ribosomal protein S28E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. Sequence homologs of S28E are found only in archaea and eukaryotes...
  7. pmc Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum
    A Yee
    Division of Molecular and Structural Biology, Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 2M9, Canada
    Proc Natl Acad Sci U S A 97:6311-5. 2000
    ..We have determined the solution structure of the RPB5 subunit from the thermophilic archeon, Methanobacterium thermoautotrophicum...
  8. ncbi A unique organization of the protein subunits of the DNA polymerase clamp loader in the archaeon Methanobacterium thermoautotrophicum deltaH
    Z Kelman
    Department of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 275:7327-36. 2000
    ..Here we report the isolation and characterization of homologues of RFC and PCNA from the archaeon, Methanobacterium thermoautotrophicum DeltaH...
  9. pmc Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase - engineering a thermostable ATP independent enzyme
    Alexander M Zhelkovsky
    New England Biolabs, Division of RNA Biology, 240 County Road, Ipswich, MA 01938 2723, USA
    BMC Mol Biol 13:24. 2012
    ..An additional useful feature is for the ligase to be active at elevated temperatures. This has the advantage or reducing preferences caused by structures of single-stranded substrates and linkers...
  10. ncbi Polymorphism and double hexamer structure in the archaeal minichromosome maintenance (MCM) helicase from Methanobacterium thermoautotrophicum
    Yacob Gómez-Llorente
    Biocomputing Unit, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Darwin 3, 28049 Madrid, Spain
    J Biol Chem 280:40909-15. 2005
    b>Methanobacterium thermoautotrophicum minichromosome maintenance complex (mtMCM), a cellular replicative helicase, is a useful model for the more complex eukaryotic MCMs...
  11. ncbi A mathematical model for the kinetics of Methanobacterium bryantii M.o.H. considering hydrogen thresholds
    Fatih Karadagli
    Department of Civil and Environmental Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208 3109, USA
    Biodegradation 18:453-64. 2007
    ..such as substrate thresholds and survival mode observed in experiments with the H2-oxidizing methanogen Methanobacterium bryantii M.o.H. We apply our model to the experimental data presented in our companion paper on H2 thresholds...
  12. pmc Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae
    J NOLLING
    Department of Microbiology, Agricultural University of Wageningen, The Netherlands
    J Bacteriol 174:5719-26. 1992
    ..system, designated MthTI, was localized on plasmid pFV1 from the thermophilic archaeon Methanobacterium thermoformicicum THF...
  13. ncbi Structural insight on the mechanism of regulation of the MarR family of proteins: high-resolution crystal structure of a transcriptional repressor from Methanobacterium thermoautotrophicum
    Vivian Saridakis
    Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada
    J Mol Biol 377:655-67. 2008
    ..Electrophoretic mobility shift assay and biophysical analyses further suggest that salicylate inactivates MTH313 and prevents it from binding to its promoter region...
  14. pmc Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum
    C D Mackereth
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Proc Natl Acad Sci U S A 97:6316-21. 2000
    ..Structure determination of this RNA polymerase subunit from Methanobacterium thermoautotrophicum reveals a topology, which we term a zinc-bundle, consisting of three alpha-helices ..
  15. ncbi Methanobacterium lacus sp. nov., isolated from the profundal sediment of a freshwater meromictic lake
    Guillaume Borrel
    Clermont Universite, Universite Blaise Pascal, Laboratoire Microorganismes Génome et Environnement, BP 10448, Clermont Ferrand, France
    Int J Syst Evol Microbiol 62:1625-9. 2012
    ..phylogenetic analyses based on 16S rRNA gene sequences, strain 17A1(T) appeared to be a member of the genus Methanobacterium, with Methanobacterium beijingense 8-2(T) (96...
  16. pmc Molecular mechanism underlying the interaction of typical Sac10b family proteins with DNA
    Yan feng Liu
    Ministry of Education Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, People s Republic of China
    PLoS ONE 7:e34986. 2012
    ..Here, we determined the crystal structure of Mth10b, an atypical member of the Sac10b family from Methanobacterium thermoautotrophicum ΔH, at 2.2 Å...
  17. ncbi Cloning, sequencing and immunological characterization of the corrinoid-containing subunit of the N5-methyltetrahydromethanopterin: coenzyme-M methyltransferase from Methanobacterium thermoautotrophicum
    E Stupperich
    Institut fur Mikrobiologie, Gottingen, Germany
    Eur J Biochem 217:115-21. 1993
    A 3.5-kb EcoRI fragment of the Methanobacterium thermoautotrophicum chromosome contains five open reading frames, mtrA to mtrE...
  18. ncbi Mutational, structural, and kinetic studies of the ATP-binding site of Methanobacterium thermoautotrophicum nicotinamide mononucleotide adenylyltransferase
    Vivian Saridakis
    Molecular and Structural Biology, Ontario Cancer Institute, University Health Network, and the Department of Medical Biophysics, University of Toronto, Ontario, Canada
    J Biol Chem 278:34356-63. 2003
    Several residues lining the ATP-binding site of Methanobacterium thermoautotrophicum nicotinamide mononucleotide adenylyltransferase (NMNATase) were mutated in an effort to better characterize their roles in substrate binding and ..
  19. ncbi The energy conserving N5-methyltetrahydromethanopterin:coenzyme M methyltransferase complex from Methanobacterium thermoautotrophicum is composed of eight different subunits
    U Harms
    Max Planck Institut fur terrestrische Mikrobiologie, Philipps Universitat, Marburg, Germany
    Eur J Biochem 228:640-8. 1995
    N5-Methyltetrahydromethanopterin:coenzyme M methyltransferase (Mtr) from Methanobacterium thermoautotrophicum strain Marburg is a membrane-associated enzyme complex which catalyzes an energy-conserving, sodium-ion-translocating step in ..
  20. pmc Halotolerance of Methanobacterium thermoautotrophicum delta H and Marburg
    R Ciulla
    Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167 3860
    J Bacteriol 176:3177-87. 1994
    b>Methanobacterium thermoautotrophicum delta H and Marburg were adapted to grow in medium containing up to 0.65 M NaCl. From 0.01 to 0.5 M NaCl, there was a lag before cell growth which increased with increasing external NaCl...
  21. ncbi Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters
    Kai Ma
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China
    Int J Syst Evol Microbiol 55:325-9. 2005
    ..9 mol%. The similarity levels of the 16S rRNA sequence of strain 8-2T with other species of the genus Methanobacterium ranged from 93.8 to 96.0 %...
  22. ncbi The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotide
    A Hochheimer
    Max Planck Institut fur terrestrische Mikrobiologie, Philipps Universitat, Marburg, Germany
    Eur J Biochem 234:910-20. 1995
    ..We report here on the primary structures of the four subunits FwdABCD of the tungsten enzyme from Methanobacterium thermoautotrophicum which were determined by cloning and sequencing the encoding genes fwdABCD...
  23. pmc Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri
    J NOLLING
    Department of Microbiology, Ohio State University, Columbus 43210, USA
    J Bacteriol 177:7238-44. 1995
    ..reductases (CH2 = H4MPT reductases), and their flanking regions have been cloned from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri and sequenced...
  24. ncbi Methanobacterium aarhusense sp. nov., a novel methanogen isolated from a marine sediment (Aarhus Bay, Denmark)
    Adris Georgis Shlimon
    Department of Microbiology, Institute of Biological Science, Aarhus University, DK 8000 Aarhus C, Denmark
    Int J Syst Evol Microbiol 54:759-63. 2004
    ..rRNA gene comparison with sequences of known methanogens, strain H2-LR(T) could be affiliated to the genus Methanobacterium. The strain forms a distinct line of descent within this genus, with Methanobacterium oryzae (95...
  25. ncbi Methanobacterium subterraneum sp. nov., a new alkaliphilic, eurythermic and halotolerant methanogen isolated from deep granitic groundwater
    S Kotelnikova
    Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Science, Pushchino na Oke, Moscow Region, Russia
    Int J Syst Bacteriol 48:357-67. 1998
    ..Phylogenetic studies based upon 16S rRNA gene sequence comparisons placed the isolate A8p in the genus Methanobacterium. Phenotypic and phylogenetic characters indicate that the alkaliphilic, halotolerant strain A8p represents a ..
  26. ncbi Geranylgeranylglyceryl phosphate synthase. Characterization of the recombinant enzyme from Methanobacterium thermoautotrophicum
    T Soderberg
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 40:14847-54. 2001
    ..The gene encoding GGGP synthase from Methanobacterium thermoautotrophicum was cloned using probes designed from the N-terminal sequence determined from the ..
  27. ncbi Purification and properties of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanobacterium thermoautotrophicum
    P Gartner
    Laboratorium für Mikrobiologie des Fachbereichs Biologie, Philipps Universitat Marburg, Germany
    Eur J Biochem 213:537-45. 1993
    ..It catalyzes an energy-conserving step in methane formation from CO2 and from acetate. The enzyme from Methanobacterium thermoautotrophicum (strain Marburg) has been purified 30-fold to apparent homogeneity...
  28. ncbi Comparison of three methyl-coenzyme M reductases from phylogenetically distant organisms: unusual amino acid modification, conservation and adaptation
    W Grabarse
    Max Planck Institut fur Biophysik, Heinrich Hoffmann Strasse 7, 60528 Frankfurt, Germany
    J Mol Biol 303:329-44. 2000
    ..growth temperature optimum, 98 degrees C) were determined and compared with the known structure of MCR from Methanobacterium thermoautotrophicum (growth temperature optimum, 65 degrees C). The active sites of MCR from M. barkeri and M...
  29. ncbi Methanobacterium petrolearium sp. nov. and Methanobacterium ferruginis sp. nov., mesophilic methanogens isolated from salty environments
    Koji Mori
    NITE Biological Resource Center, National Institute of Technology and Evaluation, 2 5 8 Kazusakamatari, Kisarazu, Chiba 292 0818, Japan
    Int J Syst Evol Microbiol 61:138-43. 2011
    ..16S rRNA gene sequence analysis revealed that strains Mic5c12(T) and Mic6c05(T) belonged to the genus Methanobacterium and their closest relative was Methanobacterium subterraneum A8p(T) (97.3 and 97...
  30. ncbi Syntrophomonas zehnderi sp. nov., an anaerobe that degrades long-chain fatty acids in co-culture with Methanobacterium formicicum
    Diana Z Sousa
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Int J Syst Evol Microbiol 57:609-15. 2007
    ..syntrophic fatty-acid-oxidizing bacterium, designated strain OL-4(T), was isolated as a co-culture with Methanobacterium formicicum DSM 1535(NT) from an anaerobic expanded granular sludge bed reactor used to treat an oleate-based ..
  31. ncbi Methanobacterium movens sp. nov. and Methanobacterium flexile sp. nov., isolated from lake sediment
    Jinxing Zhu
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China
    Int J Syst Evol Microbiol 61:2974-8. 2011
    ..Phylogenetic analysis based on 16S rRNA gene sequences placed the two isolates in the genus Methanobacterium. Strain TS-2(T) was most closely related to Methanobacterium alcaliphilum NBRC 105226(T) (96% 16S rRNA gene ..
  32. ncbi Methanobacterium thermoautotrophicum encodes two multisubunit membrane-bound [NiFe] hydrogenases. Transcription of the operons and sequence analysis of the deduced proteins
    A Tersteegen
    Max Planck Institut für Mikrobiologie, Marburg, Germany
    Eur J Biochem 264:930-43. 1999
    ..each encoding a putative multisubunit membrane-bound [NiFe] hydrogenase, were identified in the genome of Methanobacterium thermoautotrophicum...
  33. pmc Simple and efficient synthesis of 5' pre-adenylated DNA using thermostable RNA ligase
    Alexander M Zhelkovsky
    New England Biolabs, Division of RNA Biology, Ipswich, MA 01938 2723, USA
    Nucleic Acids Res 39:e117. 2011
    ..of pre-adenylated DNA linkers/adapters for next-generation sequencing using thermostable RNA ligase from Methanobacterium thermoautotrophicum (MthRnl)...
  34. pmc Coenzyme F390 synthetase from Methanobacterium thermoautotrophicum Marburg belongs to the superfamily of adenylate-forming enzymes
    P Vermeij
    Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands
    J Bacteriol 178:505-10. 1996
    ..To gain more insight into the mechanism of the former reaction, coenzyme F390 synthetase from Methanobacterium thermoautotrophicum Marburg was purified 89-fold from cell extract to a specific activity of 0.75 mumol.min-1...
  35. ncbi The open pore conformation of potassium channels
    Youxing Jiang
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nature 417:523-6. 2002
    ..The open conformation favours high conduction by compressing the membrane field to the selectivity filter, and also permits large organic cations and inactivation peptides to enter the pore from the intracellular solution...
  36. ncbi Molecular analysis of Methanobacterium phage psiM2
    P Pfister
    Institute of Microbiology, Swiss Federal Institute of Technology Zurich, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    Mol Microbiol 30:233-44. 1998
    The methanogenic archaeon Methanobacterium thermoautotrophicum Marburg is infected by the double-stranded DNA phage psiM2. The complete phage genome sequence of 26 111 bp was established...
  37. ncbi The DNA binding protein Tfx from Methanobacterium thermoautotrophicum: structure, DNA binding properties and transcriptional regulation
    A Hochheimer
    Max Planck Institut fur terrestrische Mikrobiologie, Philipps Universitat, Marburg, Germany
    Mol Microbiol 31:641-50. 1999
    In Methanobacterium thermoautotrophicum, the fmdECB operon encoding the molybdenum formyl-methanofuran dehydrogenase is directly preceded by an open reading frame tfx predicted to encode a DNA binding protein. The 16...
  38. pmc Gating and inward rectifying properties of the MthK K+ channel with and without the gating ring
    Yang Li
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Gen Physiol 129:109-20. 2007
    In MthK, a Ca2+-gated K+ channel from Methanobacterium thermoautotrophicum, eight cytoplasmic RCK domains form an octameric gating ring that controls the intracellular gate of the ion conduction pore...
  39. pmc Organization and growth phase-dependent transcription of methane genes in two regions of the Methanobacterium thermoautotrophicum genome
    J NOLLING
    Department of Microbiology, Ohio State University, Columbus 43210, USA
    J Bacteriol 177:2460-8. 1995
    Two regions of the Methanobacterium thermoautotrophicum genome containing genes that encode enzymes involved in methanogenesis (methane genes) have been cloned and sequenced to determine the extent of methane gene clustering and ..
  40. ncbi Solution Structure of MTH0776 from Methanobacterium thermoautotrophicum
    Godwin Amegbey
    Department of Computing Science and Biological Sciences, University of Alberta, Edmonton, Alberta, Canada, T6G 2E8
    J Biomol NMR 33:51-6. 2005
  41. ncbi Crystal structure of Methanobacterium thermoautotrophicum phosphoribosyl-AMP cyclohydrolase HisI
    J Sivaraman
    Biotechnology Research Institute, National Research Council, Montreal, Quebec H4P 2R2, Canada, Montreal
    Biochemistry 44:10071-80. 2005
    ..The three-dimensional structure of PR-AMP cyclohydrolase from Methanobacterium thermoautotrophicum was solved and refined to 1.7 A resolution. The enzyme is a homodimer...
  42. ncbi The heterodisulfide reductase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic of pyridine-nucleotide-dependent thioredoxin reductases
    R Hedderich
    Max Planck Institut für terrestrische Mikrobiologie und Laboratorium für Mikrobiologie des Fachbereichs Biologie, Philipps Universitat Marburg, Germany
    Eur J Biochem 225:253-61. 1994
    ..46 kDa) did not show sequence similarity to other known proteins, but appears to possess a C-terminal hydrophobic alpha-helix that might function as a membrane anchor. Although hdrB and hdrC are juxtaposed, these genes are not near hdrA...
  43. pmc Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics
    D R Smith
    Genome Therapeutics Corporation, Collaborative Research Division, Waltham, Massachusetts 02154, USA
    J Bacteriol 179:7135-55. 1997
    The complete 1,751,377-bp sequence of the genome of the thermophilic archaeon Methanobacterium thermoautotrophicum deltaH has been determined by a whole-genome shotgun sequencing approach...
  44. pmc Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA
    Christopher Torchia
    Department of Biological Sciences and Department of Microbiology and Immunology, State University of New York at Buffalo, Buffalo, NY 14260, USA
    Nucleic Acids Res 36:6218-27. 2008
    ..Here, we report the characterization of an RNA ligase from the thermophilic archaeon, Methanobacterium thermoautotrophicum...
  45. pmc Component A2 of methylcoenzyme M reductase system from Methanobacterium thermoautotrophicum delta H: nucleotide sequence and functional expression by Escherichia coli
    C H Kuhner
    Department of Microbiology, University of Illinois, Urbana 61801
    J Bacteriol 175:3195-203. 1993
    The gene for component A2 of the methylcoenzyme M reductase system from Methanobacterium thermoautotrophicum delta H was cloned, and its nucleotide sequence was determined...
  46. ncbi H2: heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum. Composition and properties
    E Setzke
    Max Planck Institut für Terrestrsche Mikrobiologie, Marburg, Germany
    Eur J Biochem 220:139-48. 1994
    ..In this study, we show that in Methanobacterium thermoautotrophicum (strain Marburg) this reaction is catalyzed by a stable H2-heterodisulfide oxidoreductase ..
  47. ncbi The molybdenum formylmethanofuran dehydrogenase operon and the tungsten formylmethanofuran dehydrogenase operon from Methanobacterium thermoautotrophicum. Structures and transcriptional regulation
    A Hochheimer
    Max Planck Institut fur terrestrische Mikrobiologie, Philipps Universitat Marburg, Germany
    Eur J Biochem 242:156-62. 1996
    b>Methanobacterium thermoautotrophicum contains a tungsten formylmethanofuran dehydrogenase (FwdABCD) and a molybdenum formylmethanofuran dehydrogenase (FmdABC)...
  48. pmc Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245
    J NOLLING
    Department of Microbiology, The Ohio State University, Columbus 43210, USA
    J Bacteriol 179:899-908. 1997
    The formate dehydrogenase-encoding fdhCAB operon and flanking genes have been cloned and sequenced from Methanobacterium thermoformicicum Z-245...
  49. ncbi Primary structure of cyclohydrolase (Mch) from Methanobacterium thermoautotrophicum (strain Marburg) and functional expression of the mch gene in Escherichia coli
    M Vaupel
    Max Planck Institut fur terrestrische Mikrobiologie, Marburg, Germany
    Eur J Biochem 236:294-300. 1996
    ..The mch gene was overexpressed in Escherichia coli yielding an active enzyme of 37 kDa with a specific activity of 30 U/mg cell extract protein...
  50. ncbi Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH
    Z Kelman
    Department of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 274:28751-61. 1999
    We describe here the isolation and characterization of a B-type DNA polymerase (PolB) from the archaeon Methanobacterium thermoautotrophicum DeltaH. Uniquely, the catalytic domains of M...
  51. pmc Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta H
    T D Pihl
    Department of Microbiology, Ohio State University, Columbus 43210
    J Bacteriol 176:6384-91. 1994
    The genes encoding the two isoenzymes of methyl coenzyme M reductase (MRI and MRII) in Methanobacterium thermoautotrophicum delta H have been cloned and sequenced...
  52. ncbi Purification, regulation, and molecular and biochemical characterization of pyruvate carboxylase from Methanobacterium thermoautotrophicum strain deltaH
    B Mukhopadhyay
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 273:5155-66. 1998
    We discovered that Methanobacterium thermoautotrophicum strain DeltaH possessed pyruvate carboxylase (PYC), and this biotin prototroph required exogenously supplied biotin to exhibit detectable amounts of PYC activity...
  53. ncbi Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methano
    A Wasserfallen
    Mikrobiologisches Institut, Swiss Federal Institute of Technology, Zurich
    Int J Syst Evol Microbiol 50:43-53. 2000
    ..of new and published data, the phylogenetic position of 18 thermophilic isolates currently classified as Methanobacterium species was reinvestigated...
  54. ncbi Structures of the MthK RCK domain and the effect of Ca2+ on gating ring stability
    Jianbo Dong
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    J Biol Chem 280:41716-24. 2005
    MthK is a Ca2+-gated K+ channel from Methanobacterium autotrophicum. The crystal structure of the MthK channel in a Ca2+-bound open state was previously determined at 3...
  55. pmc The Methanobacterium thermoautotrophicum MCM protein can form heptameric rings
    Xiong Yu
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    EMBO Rep 3:792-7. 2002
    ..In most archaea a single MCM protein exists. The protein from Methanobacterium thermoautotrophicum (mtMCM) has been reported to assemble into a large complex consistent with a dodecamer...
  56. ncbi Double hexamer disruption and biochemical activities of Methanobacterium thermoautotrophicum MCM
    Ryan J Fletcher
    Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089, USA
    J Biol Chem 280:42405-10. 2005
    b>Methanobacterium thermoautotrophicum MCM (mtMCM) is a helicase required for DNA replication. Previous electron microscopy studies have shown mtMCM in several oligomeric forms...
  57. pmc TFE, an archaeal transcription factor in Methanobacterium thermoautotrophicum related to eucaryal transcription factor TFIIEalpha
    B L Hanzelka
    Department of Microbiology, The Ohio State University, Columbus, Ohio 43210, USA
    J Bacteriol 183:1813-8. 2001
    In the archaeon Methanobacterium thermoautotrophicum, MTH1669 encodes a protein with a sequence related to the N-terminal sequences of the alpha-subunits of eucaryal general transcription factor TFIIE...
  58. ncbi Crystal structure of dTDP-4-keto-6-deoxy-D-hexulose 3,5-epimerase from Methanobacterium thermoautotrophicum complexed with dTDP
    D Christendat
    Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Ontario M5G 2M9, Canada
    J Biol Chem 275:24608-12. 2000
    ..The crystal structure of RmlC from Methanobacterium thermoautotrophicum was determined in the presence and absence of dTDP, a substrate analogue...
  59. ncbi Endonuclease IV enhances base excision repair of endonuclease III from Methanobacterium thermoautotrophicum
    Jung Ho Back
    Structural Biology Research Center, Korea Institute of Science and Technology, P O BOX 131, Cheongryang, Seoul, South Korea
    DNA Repair (Amst) 2:455-70. 2003
    ..We expressed and characterized two putative endonuclease genes from Methanobacterium thermoautotrophicum, Mt0764 and Mt1010, encoding homologues of endonuclease III (endo III) and endonuclease ..
  60. ncbi Crystal structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum
    P Strop
    Biochemistry Option, California Institute of Technology, Pasadena, California 91125, USA
    J Biol Chem 276:10299-305. 2001
    The structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum (Cab) has been determined to 2.1-A resolution using the multiwavelength anomalous diffraction phasing technique...
  61. ncbi Insights into ligand binding and catalysis of a central step in NAD+ synthesis: structures of Methanobacterium thermoautotrophicum NMN adenylyltransferase complexes
    V Saridakis
    Division of Molecular and Structural Biology, Ontario Cancer Institute, 610 University Avenue Toronto M5G 2M9, Ontario, Canada
    J Biol Chem 276:7225-32. 2001
    ..The structure of Methanobacterium thermoautotrophicum NMNATase determined using multiwavelength anomalous dispersion phasing revealed a ..
  62. ncbi Modulation of MthK potassium channel activity at the intracellular entrance to the pore
    Lyubov V Parfenova
    Department of Physiology, University of Texas Health Science Center, San Antonio, 78229, USA
    J Biol Chem 281:21131-8. 2006
    ..with the LB2003 K(+) uptake-deficient strain of Escherichia coli to analyze residues that are critical to Methanobacterium thermoautotrophicum potassium channel (MthK) function...
  63. pmc Calcium-dependent gating of MthK, a prokaryotic potassium channel
    Brittany Zadek
    Department of Biochemistry and Membrane Biology, University of California, Davis, 95616, USA
    J Gen Physiol 127:673-85. 2006
    ..We were able to capture the mechanistic features of MthK with a modified MWC model...
  64. pmc Regulation of tryptophan biosynthesis in Methanobacterium thermoautotrophicum Marburg
    D A Gast
    Mikrobiologisches Institut, Swiss Federal Institute of Technology, ETH Zentrum, Zurich
    J Bacteriol 176:4590-6. 1994
    A tryptophan-auxotrophic mutant of the archaeon Methanobacterium thermoautotrophicum Marburg was grown with growth-promoting and growth-limiting concentrations of tryptophan...
  65. ncbi Crystal structure and mechanism of a calcium-gated potassium channel
    Youxing Jiang
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nature 417:515-22. 2002
    ..expressed, analysed electrical properties, and determined the crystal structure of a K(+) channel (MthK) from Methanobacterium thermoautotrophicum in the Ca(2+)-bound, opened state...
  66. ncbi How to gate an ion channel: lessons from MthK
    Sudha Chakrapani
    Nat Struct Mol Biol 14:180-2. 2007
  67. ncbi The purification, characterization, and primary structure of a small redox protein from Methanobacterium thermoautotrophicum, an archaebacterium
    S C McFarlan
    Department of Biochemistry, Medical School, University of Minnesota, Minneapolis 55455
    J Biol Chem 267:10561-9. 1992
    ..has been purified to homogeneity from cell-free extracts of the strictly anaerobic thermophilic methanogen, Methanobacterium thermoautotrophicum (strain Marburg)...
  68. pmc Identification of the CTAG-recognizing restriction-modification systems MthZI and MthFI from Methanobacterium thermoformicicum and characterization of the plasmid-encoded mthZIM gene
    J NOLLING
    Department of Microbiology, Wageningen Agricultural University, The Netherlands
    Nucleic Acids Res 20:5047-52. 1992
    ..and modification (R/M) systems, designated MthZI and MthFI, were identified in the thermophilic archaeon Methanobacterium thermoformicicum strains Z-245 and FTF, respectively...
  69. ncbi Characterization of a 45-kDa flavoprotein and evidence for a rubredoxin, two proteins that could participate in electron transport from H2 to CO2 in methanogenesis in Methanobacterium thermoautotrophicum
    J NOLLING
    Department of Microbiology, Ohio State University, Columbus, USA
    Eur J Biochem 231:628-38. 1995
    b>Methanobacterium thermoautotrophicum strains contain a flavoprotein (flavoprotein A) that copurifies with the H2:heterodisulfide oxidoreductase complex...
  70. pmc Structure of the archaeal translation initiation factor aIF2 beta from Methanobacterium thermoautotrophicum: implications for translation initiation
    Pablo Gutierrez
    McGill University, Department of Biochemistry, McIntyre Medical Science Building, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada
    Protein Sci 13:659-67. 2004
    ..We have determined the solution structure of the intact beta-subunit of aIF2 from Methanobacterium thermoautotrophicum...
  71. pmc The solution structure of ribosomal protein S17E from Methanobacterium thermoautotrophicum: a structural homolog of the FF domain
    Bin Wu
    Division of Cancer Genomics and Proteomics, Ontario Cancer Institute, Toronto, Ontario M5G 2M9, Canada
    Protein Sci 17:583-8. 2008
    The ribosomal protein S17E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. S17E is a 62-residue protein conserved in archaea and eukaryotes and has no counterparts in bacteria...
  72. ncbi Biochemical activities of the BOB1 mutant in Methanobacterium thermoautotrophicum MCM
    Ryan J Fletcher
    Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089, USA
    Biochemistry 45:462-7. 2006
    Minichromosomal maintenance proteins (MCMs) are considered to be the replicative helicase. Methanobacterium thermoautotrophicum has a single MCM gene (mtMCM). The crystal structure of the mtMCM N-terminal region is a double hexamer...
  73. ncbi The formylmethanofuran dehydrogenase isoenzymes in Methanobacterium wolfei and Methanobacterium thermoautotrophicum: induction of the molybdenum isoenzyme by molybdate and constitutive synthesis of the tungsten isoenzyme
    A Hochheimer
    Max Planck Institut fur terrestrische Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Arch Microbiol 170:389-93. 1998
    ..b>Methanobacterium wolfei and Methanobacterium thermoautotrophicum have been shown to contain two isoenzymes, a tungsten-..
  74. ncbi Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea
    M Nishihara
    Department of Chemistry, University of Occupational and Environmental Health, Japan, Kitakyushu
    J Biochem 122:572-6. 1997
    ..backbone (sn-glycerol-1-phosphate) of archaeal ether lipids was purified from a methanogenic archaeon, Methanobacterium thermoautotrophicum, and characterized...
  75. pmc Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with Desulfovibrio vulgaris Hildenborough and Methanobacterium congolense: global transcriptomic and proteomic analyses
    Yujie Men
    Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720 1710, USA
    ISME J 6:410-21. 2012
    ..vulgaris Hildenborough (DVH) as a co-culture, as well as with DVH and the hydrogenotrophic methanogen Methanobacterium congolense (MC) as a tri-culture using lactate as the sole energy and carbon source...
  76. ncbi MTH187 from Methanobacterium thermoautotrophicum has three HEAT-like repeats
    Olivier Julien
    Département de biochimie et de microbiologie and CREFSIP, Universite Laval, 3255 pav Marchand, Quebec, G1K 7P4, Canada
    J Biomol NMR 35:149-54. 2006
    ..is one of the 424 non-membrane proteins that were cloned and purified for the structural proteomic project of Methanobacterium thermoautotrophicum [Christendat, D., Yee, A., Dharamsi, A., Kluger, Y., Gerstein, M., Arrowsmith, C.H...
  77. ncbi Structure-based functional classification of hypothetical protein MTH538 from Methanobacterium thermoautotrophicum
    J R Cort
    Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, EMSL 2569 K8 98, Richland, WA 99352, USA
    J Mol Biol 302:189-203. 2000
    The structure of MTH538, a previously uncharacterized hypothetical protein from Methanobacterium thermoautotrophicum, has been determined by NMR spectroscopy...
  78. ncbi Enzymatic degradation of cyclic 2,3-diphosphoglycerate to 2,3-diphosphoglycerate in Methanobacterium thermoautotrophicum
    M V Sastry
    Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02167
    Biochemistry 31:2926-35. 1992
    ..to be the product of the enzymatic degradation of cyclic 2,3-diphosphoglycerate (cDPG) in the archaebacterium Methanobacterium thermoautotrophicum delta H...
  79. pmc Cyclic 2,3-diphosphoglycerate as a component of a new branch in gluconeogenesis in Methanobacterium thermoautotrophicum delta H
    A Gorkovenko
    Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167
    J Bacteriol 175:4087-95. 1993
    A unique compound, cyclic 2,3-diphosphoglycerate (cDPG), is the major soluble carbon and phosphorus solute in Methanobacterium thermoautotrophicum delta H under optimal conditions of cell growth...
  80. ncbi Kinetics and binding sites for interaction of the prefoldin with a group II chaperonin: contiguous non-native substrate and chaperonin binding sites in the archaeal prefoldin
    Mina Okochi
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    J Biol Chem 279:31788-95. 2004
    ..Our data are consistent with substrate and chaperonin binding sites on prefoldin that are different but in close proximity, which suggests a possible handover mechanism of prefoldin substrates to the chaperonin...
  81. ncbi Structural diversity and plasticity associated with nucleotides targeting orotidine monophosphate decarboxylase
    Ewa Poduch
    Center for Molecular Design and Preformulations, Toronto General Research Institute, Toronto General Hospital, Toronto, ON, Canada
    J Med Chem 51:432-8. 2008
    ..the kinetic properties of eight common and endogenous nucleotides with ODCases from three species: Methanobacterium thermoautotrophicum, Plasmodium falciparum, and Homo sapiens...
  82. ncbi Ion channels: an open and shut case
    Maria Schumacher
    Nature 417:501-2. 2002
  83. pmc The crystal structure of hypothetical protein MTH1491 from Methanobacterium thermoautotrophicum
    Dinesh Christendat
    Clinical Genomics Center, University Health Network, Toronto, Ontario, M5G 1L7, Canada
    Protein Sci 11:1409-14. 2002
    ..we have determined the crystal structure of MTH1491, a previously uncharacterized hypothetical protein from Methanobacterium thermoautotrophicum...
  84. ncbi Multidomain initiation factor 2 from Thermus thermophilus consists of the individual autonomous domains
    Gabriel Zoldak
    Department of Biochemistry, Faculty of Sciences, P J Safarik University, Kosice, Slovakia
    Biochemistry 47:4992-5005. 2008
    The three-dimensional chalice-like crystal structure of initiation factor 2 IF2/eIF5B from Methanobacterium thermoautotrophicum represents a novel fold and domain architecture in which the N-terminal G domain and the C-terminal C domain ..
  85. ncbi Crystal structure of Methanobacterium thermoautotrophicum conserved protein MTH1020 reveals an NTN-hydrolase fold
    Vivian Saridakis
    Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Ontario, Canada
    Proteins 48:141-3. 2002
  86. ncbi Crystal structures of inhibitor complexes reveal an alternate binding mode in orotidine-5'-monophosphate decarboxylase
    Ning Wu
    Department of Biochemistry, University of Toronto, Ontario M5S 1A8, Canada
    J Biol Chem 277:28080-7. 2002
    The crystal structures of the enzyme orotidine-5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with its product UMP and the inhibitors 6-hydroxyuridine 5'-phosphate (BMP), XMP, and CMP are reported...
  87. ncbi Solution structure of the hypothetical protein MTH0637 from Methanobacterium thermoautotrophicum
    Antonio Pineda-Lucena
    J Biomol NMR 22:291-4. 2002
  88. ncbi Crystal structure of human nicotinamide mononucleotide adenylyltransferase in complex with NMN
    Erik Werner
    Crystallography Group, Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13092, Berlin, Germany
    FEBS Lett 516:239-44. 2002
    ..Human NMNAT displays a different oligomerization compared to the archaeal enzyme. The protein-nicotinamide mononucleotide interaction pattern provides insight into ligand binding in the human enzyme...
  89. ncbi A mechanism from quantum chemical studies for methane formation in methanogenesis
    Vladimir Pelmenschikov
    Department of Physics, Stockholm University, Box 6730, S 113 85 Stockholm, Sweden
    J Am Chem Soc 124:4039-49. 2002
    ..Heterodisulfide CoB-S-S-CoM formation is proposed in the final step in which nickel is reduced back to Ni(I). The suggested mechanism agrees well with experimental observations...
  90. ncbi Substrate binding induces domain movements in orotidine 5'-monophosphate decarboxylase
    Pernille Harris
    Centre for Crystallographic Studies, Department of Chemistry, University of Copenhagen, Universitetsparken 5, Denmark
    J Mol Biol 318:1019-29. 2002
    ..presented herein, of the two Saccharomyces cerevisiae ODCase structures (with and without BMP) and the Methanobacterium thermoautotrophicum ODCase structures (with and without 6-aza UMP) show very similar, but somewhat smaller ..
  91. pmc HMt, a histone-related protein from Methanobacterium thermoautotrophicum delta H
    R Tabassum
    Department of Microbiology, Ohio State University, Columbus 43210
    J Bacteriol 174:7890-5. 1992
    HMt, a histone-related protein, has been isolated and characterized from Methanobacterium thermoautotrophicum delta H...
  92. pmc Automated error-tolerant macromolecular structure determination from multidimensional nuclear Overhauser enhancement spectra and chemical shift assignments: improved robustness and performance of the PASD algorithm
    John J Kuszewski
    Imaging Sciences Laboratory, Center for Information Technology, National Institutes of Health, Bethesda, MD 20892 5624, USA
    J Biomol NMR 41:221-39. 2008
    ..We show that useful assignment information can be obtained even in the case in which a unique structure cannot be determined...
  93. ncbi Structural and functional characterization of a thioredoxin-like protein (Mt0807) from Methanobacterium thermoautotrophicum
    Godwin Y Amegbey
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada T6G 2N8
    Biochemistry 42:8001-10. 2003
    Mt0807 is an 85-residue thiol-redox protein from the anaerobic archaebacterium Methanobacterium thermoautotrophicum...
  94. ncbi Data mining crystallization databases: knowledge-based approaches to optimize protein crystal screens
    Matthew S Kimber
    Affinium Pharmaceuticals Inc, Toronto, Ontario, Canada
    Proteins 51:562-8. 2003
    ....
  95. pmc Solution structure of the hypothetical protein Mth677 from Methanobacterium thermoautotrophicum: a novel alpha+beta fold
    Francisco J Blanco
    NMR Group, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain
    Protein Sci 13:1458-65. 2004
    The structure of Mth677, a hypothetical protein from Methanobacterium thermoautotrophicum (Mth), has been determined by using heteronuclear nuclear magnetic resonance (NMR) methods on a double-labeled (15)N-(13)C sample...
  96. ncbi Three-dimensional structures of fibrillar Sm proteins: Hfq and other Sm-like proteins
    Veronique Arluison
    Institut de Biologie Physico Chimique, CNRS UPR 9073 conventionnee avec l université Paris 7, 13 rue P et M Curie, 75005 Paris, France
    J Mol Biol 356:86-96. 2006
    ..The different fibrillar ultrastructures formed by Hfq and SmAP are presented and examined herein, with the overall goal of elucidating another similarity amongst the diverse members of the Sm protein family...
  97. ncbi The structure and function of MCM from archaeal M. Thermoautotrophicum
    Ryan J Fletcher
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Science Center, School of Medicine, Denver, Colorado 80262, USA
    Nat Struct Biol 10:160-7. 2003
    ..We have determined the structure of a fragment of MCM from Methanobacterium thermoautotrophicum (mtMCM), a model system for eukaryotic MCM...
  98. ncbi Crystal structure of the putative adapter protein MTH1859
    Hong Ye
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    J Struct Biol 148:251-6. 2004
    MTH1859 from Methanobacterium thermoautotrophicum is a 77 residue protein representing a conserved family of functionally uncharacterized proteins...
  99. ncbi The crystal structure of MT0146/CbiT suggests that the putative precorrin-8w decarboxylase is a methyltransferase
    Jacob P Keller
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA
    Structure 10:1475-87. 2002
    ..We herein present the crystal structure of MT0146, the CbiT homolog from Methanobacterium thermoautotrophicum...
  100. ncbi Crystal structure of MTH169, a crucial component of phosphoribosylformylglycinamidine synthetase
    Renu Batra
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Proteins 49:285-8. 2002
  101. ncbi Identification of a novel archaebacterial thioredoxin: determination of function through structure
    Sudeepa Bhattacharyya
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
    Biochemistry 41:4760-70. 2002
    ..and ascertain the function of a previously unknown, conserved protein (MtH895) from the thermophilic archeon Methanobacterium thermoautotrophicum...

Research Grants9

  1. Gating Mechanisms of the Prokaryotic Calcium Activated Potassium channel MthK
    DAVID JOHN POSSON; Fiscal Year: 2010
    ..MthK is a prokaryotic K+ channel from Methanobacterium thermoautotrophicum that is representative of a family of channels activated by calcium...
  2. Molecular Mechanism of Cation Channel Selectivity
    Youxing Jiang; Fiscal Year: 2013
    ..other is K+ selective channels, using a K+ selective NaK mutant (NaK2K) and the MthK K+ channel from Methanobacterium thermoautotrophicum as model systems...
  3. BIOSYNTHESIS OF RIBOFLAVIN AND RELATED COMPOUNDS
    HEINZ FLOSS; Fiscal Year: 1991
    ..Finally, the biosynthesis of 8-hydroxy-7,8-bisnor-5- deazariboflavin in Streptomyces will be investigated and the results will be compared to those in Methanobacterium thermoautotrophicum, another producer of this 5-deazaflavin.
  4. Mechanism of Ligand Gating in Potassium Channels
    Youxing Jiang; Fiscal Year: 2008
    ..proven to be an excellent model system, a Ca2+-activated K+ channel called MthK, from the archaebacterium Methanobacterium thermoautotrophicum...
  5. Mechanism of KDO 8-P and DAH 7-P Synthase
    RONALD WOODARD; Fiscal Year: 2004
    ....
  6. ENZYMOLOGY OF ACETOTROPHIC METHANOGENESIS
    JAMES FERRY; Fiscal Year: 2003
    ..The results are expected to contribute not only to a deeper understanding of the acetate fermentation pathway, but more importantly to the broader field of anaerobic microbiology and beyond. ..
  7. Probing Microb Comm - Stable Isotope & RNA/DNA Analyses
    James Tiedje; Fiscal Year: 2004
    ..abstract_text> ..
  8. High-Accuracy Models of Proteins from Remote Homology
    Jie Liang; Fiscal Year: 2009
    ..These computational capabilities could have profound impacts to drug design and disease studies. ..
  9. Mechanism of A5P Isomerase
    RONALD WESLEY WOODARD; Fiscal Year: 2010
    ..The ultimate goal of these studies is to better understand the role of LPS, a critical macromolecule essential to both the survival and virulence of Gram-(-) microorganisms ..