Genomes and Genes
Summary: A genus of aerobic, chemolithotrophic, coccoid ARCHAEA whose organisms are thermoacidophilic. Its cells are highly irregular in shape, often lobed, but occasionally spherical. It has worldwide distribution with organisms isolated from hot acidic soils and water. Sulfur is used as an energy source.
Publications276 found, 100 shown here
- CRISPR families of the crenarchaeal genus Sulfolobus: bidirectional transcription and dynamic propertiesReidun K Lillestøl
Centre for Comparative Genomics, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark
Mol Microbiol 72:259-72. 2009Clusters of regularly interspaced short palindromic repeats (CRISPRs) of Sulfolobus fall into three main families based on their repeats, leader regions, associated cas genes and putative recognition sequences on viruses and plasmids...
- A novel interference mechanism by a type IIIB CRISPR-Cmr module in SulfolobusLing Deng
Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK2200, Copenhagen N, Denmark
Mol Microbiol 87:1088-99. 2013..The archaeon Sulfolobus islandicus REY15A encodes three interference modules, one of type IA and two of type IIIB...
- Viral biogeography revealed by signatures in Sulfolobus islandicus genomesNicole L Held
Department of Microbiology, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
Environ Microbiol 11:457-66. 2009..The biogeographic distribution of viruses that infect Sulfolobus islandicus is investigated using genome comparisons among four newly identified, integrated, Sulfolobus spindle-..
- Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in SulfolobusLing Deng
Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen K, Denmark
Nucleic Acids Res 40:2470-80. 2012..cbp1 gene deletion from Sulfolobus islandicus REY15A produced a strong reduction in pre-crRNA yields from CRISPR loci but did not inhibit the ..
- Genome analyses of Icelandic strains of Sulfolobus islandicus, model organisms for genetic and virus-host interaction studiesLi Guo
Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, DK 2200N Copenhagen, Denmark
J Bacteriol 193:1672-80. 2011The genomes of two Sulfolobus islandicus strains obtained from Icelandic solfataras were sequenced and analyzed. Strain REY15A is a host for a versatile genetic toolbox...
- DNA bending, compaction and negative supercoiling by the architectural protein Sso7d of Sulfolobus solfataricusAlessandra Napoli
Institute of Protein Biochemistry and Enzymology, Consiglio Nazionale delle Ricerche, Via Pietro Castellino 111, 80131 Naples, Italy
Nucleic Acids Res 30:2656-62. 2002..the Sso7d/Sac7d family are small, abundant, non-specific DNA-binding proteins of the hyperthermophilic Archaea SULFOLOBUS: Crystal structures of these proteins in complex with oligonucleotides showed that they induce changes in the ..
- Patterns of gene flow define species of thermophilic ArchaeaHinsby Cadillo-Quiroz
Department of Microbiology and Institute for Genomic Biology, University of Illinois, Urbana Champaign, Urbana, Illinois, United States of America
PLoS Biol 10:e1001265. 2012..Here we use high-throughput genome sequencing to identify ongoing speciation in the thermoacidophilic Archaeon Sulfolobus islandicus...
- How oligomerization contributes to the thermostability of an archaeon protein. Protein L-isoaspartyl-O-methyltransferase from Sulfolobus tokodaiiYoshikazu Tanaka
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba yama 07, Aoba ku, Sendai 980 8579, Japan
J Biol Chem 279:32957-67. 2004..of archaeon proteins, we focused on a hexameric protein, protein L-isoaspartyl-O-methyltransferase from Sulfolobus tokodaii (StoPIMT)...
- Structure of Hjc, a Holliday junction resolvase, from Sulfolobus solfataricusC S Bond
Wellcome Trust Biocentre, University of Dundee, Dundee, Tayside DD1 5EH, United Kingdom
Proc Natl Acad Sci U S A 98:5509-14. 2001The 2.15-A structure of Hjc, a Holliday junction-resolving enzyme from the archaeon Sulfolobus solfataricus, reveals extensive structural homology with a superfamily of nucleases that includes type II restriction enzymes...
- Structural and enzymatic characterization of the lactonase SisLac from Sulfolobus islandicusJulien Hiblot
URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
PLoS ONE 7:e47028. 2012A new member of the Phosphotriesterase-Like Lactonases (PLL) family from the hyperthermophilic archeon Sulfolobus islandicus (SisLac) has been characterized...
- Genetic manipulation in Sulfolobus islandicus and functional analysis of DNA repair genesChangyi Zhang
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, People s Republic of China
Biochem Soc Trans 41:405-10. 2013Recently, a novel gene-deletion method was developed for the crenarchaeal model Sulfolobus islandicus, which is a suitable tool for addressing gene essentiality in depth...
- Reverse gyrase gene from Sulfolobus shibatae B12: gene structure, transcription unit and comparative sequence analysis of the two domainsC Jaxel
Institut de Génétique et de Microbiologie, URA 1354, Universite Paris Sud, Orsay, France
Nucleic Acids Res 24:4668-75. 1996We cloned and sequenced a DNA fragment from the thermophilic archaeal strain Sulfolobus shibatae B12 that includes the gene topR encoding the reverse gyrase...
- The DNA primase of Sulfolobus solfataricus is activated by substrates containing a thymine-rich bubble and has a 3'-terminal nucleotidyl-transferase activityMariarosaria De Falco
Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via P Castellino, 111, 80131 Napoli, Italy
Nucleic Acids Res 32:5223-30. 2004..and biochemical characterization of a DNA primase (Sso DNA primase) from the thermoacidophilic crenarchaeon Sulfolobus solfataricus...
- Genetic requirements for the function of the archaeal virus SSV1 in Sulfolobus solfataricus: construction and testing of viral shuttle vectorsK M Stedman
Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
Genetics 152:1397-405. 1999..ORF) disruption and a serial selection technique in Escherichia coli and the extremely thermophilic archaeon Sulfolobus solfataricus allowed the identification of otherwise cryptic crucial and noncrucial viral open reading frames in ..
- Occurrence and characterization of mercury resistance in the hyperthermophilic archaeon Sulfolobus solfataricus by use of gene disruptionJames Schelert
Beadle Center for Genetics, University of Nebraska, Lincoln, Nebraska 68588 0666, USA
J Bacteriol 186:427-37. 2004..The archaeon Sulfolobus solfataricus was sensitive to mercuric chloride, and low-level adaptive resistance could be induced by metal ..
- The complete genome of the crenarchaeon Sulfolobus solfataricus P2Q She
Microbial Genome Group, Institute of Molecular Biology, University of Copenhagen, , DK-1307 Copenhagen, Denmark
Proc Natl Acad Sci U S A 98:7835-40. 2001The genome of the crenarchaeon Sulfolobus solfataricus P2 contains 2,992,245 bp on a single chromosome and encodes 2,977 proteins and many RNAs. One-third of the encoded proteins have no detectable homologs in other sequenced genomes...
- The interaction of Alba, a conserved archaeal chromatin protein, with Sir2 and its regulation by acetylationStephen D Bell
Medical Research Council MRC Cancer Cell Unit, The Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 2QH, UK
Science 296:148-51. 2002..These data provide a paradigm for how Sir2 family proteins influence transcription and suggest that modulation of chromatin structure by acetylation arose before the divergence of the archaeal and eukaryotic lineages...
- Copper tolerance of the thermoacidophilic archaeon Sulfolobus metallicus: possible role of polyphosphate metabolismFrancisco Remonsellez
Laboratory of Molecular Microbiology and Biotechnology, Department of Biology, Faculty of Sciences, University of Chile, Santiago 1, Casilla 653, Santiago, Chile
Microbiology 152:59-66. 2006..by the electron energy loss spectroscopy (EELS) procedure and quantified by using specific enzymic methods in Sulfolobus acidocaldarius, Sulfolobus metallicus and Sulfolobus solfataricus...
- UV-inducible DNA exchange in hyperthermophilic archaea mediated by type IV piliMałgorzata Ajon
Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9749 AG Groningen, The Netherlands
Mol Microbiol 82:807-17. 2011..Knockout strains of Sulfolobus acidocaldarius incapable of pilus production could not self-aggregate, but were partners in mating experiments ..
- Biogeography of the Sulfolobus islandicus pan-genomeMichael L Reno
Department of Microbiology and Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
Proc Natl Acad Sci U S A 106:8605-10. 2009..Through population-scale comparative genomics of 7 Sulfolobus islandicus genomes from 3 locations, we demonstrate the biogeographical structure of the pan-genome of this ..
- Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicusQunxin She
Danish Archaea Centre, Department of Biology, University of Copenhagen, Biocenter, Ole Maaloes Vej 5, DK 2200 Copenhagen N, Denmark
Biochem Soc Trans 37:92-6. 2009b>Sulfolobus belongs to the hyperthermophilic archaea and it serves as a model organism to study archaeal molecular biology and evolution...
- Reverse gyrase recruitment to DNA after UV light irradiation in Sulfolobus solfataricusAlessandra Napoli
Institute of Protein Biochemistry, Consiglio Nazionale delle Ricerche, Via P Castellino 111, 80131 Naples, Italy
J Biol Chem 279:33192-8. 2004..We show here that reverse gyrase of the archaean Sulfolobus solfataricus is mobilized to DNA in vivo after UV irradiation...
- Identification and properties of the crenarchaeal single-stranded DNA binding protein from Sulfolobus solfataricusR I Wadsworth
Centre for Biomolecular Science, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK
Nucleic Acids Res 29:914-20. 2001..b>Sulfolobus SSB lacks the zinc finger motif found in the eucaryal and euryarchaeal proteins, possessing instead a flexible C-..
- CRISPR-mediated defense mechanisms in the hyperthermophilic archaeal genus SulfolobusAndrea Manica
University of Vienna, Department of Genetics in Ecology, Vienna, Austria
RNA Biol 10:671-8. 2013..Archaeal genomes and, in particular, organisms of the extremely thermoacidophilic genus Sulfolobus, carry extensive CRISPR loci each with dozens of sequence signatures (spacers) able to mediate targeting and ..
- Molecular and structural basis of ESCRT-III recruitment to membranes during archaeal cell divisionRachel Y Samson
Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK
Mol Cell 41:186-96. 2011Members of the crenarchaeal kingdom, such as Sulfolobus, divide by binary fission yet lack genes for the otherwise near-ubiquitous tubulin and actin superfamilies of cytoskeletal proteins...
- Exceptional virion release mechanism: one more surprise from archaeal virusesDavid Prangishvili
Institut Pasteur, Department of Microbiology, Biologie Moléculaire du Gène chez les Extrêmophiles, 75015 Paris, France
Curr Opin Microbiol 14:315-20. 2011..Here we summarize available data on the extraordinary egress mechanism exploited by the Sulfolobus islandicus rod-shaped virus SIRV2 and the Sulfolobus turreted icosahedral virus STIV...
- A novel virus family, the Rudiviridae: Structure, virus-host interactions and genome variability of the sulfolobus viruses SIRV1 and SIRV2D Prangishvili
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
Genetics 152:1387-96. 1999The unenveloped, stiff-rod-shaped, linear double-stranded DNA viruses SIRV1 and SIRV2 from Icelandic Sulfolobus isolates form a novel virus family, the Rudiviridae. The sizes of the genomes are 32. 3 kbp for SIRV1 and 35.8 kbp for SIRV2...
- Cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium Sulfolobus acidocaldariusK Maruta
Hayashibara Biochemical Laboratories, Inc, Okayama, Japan
Biochim Biophys Acta 1291:177-81. 1996..enzyme (TreX), and maltooligosyltrehalose synthase (TreY) have been cloned from the thermophilic archaebacterium Sulfolobus acidocaldarius ATCC33909...
- Targeted disruption of the alpha-amylase gene in the hyperthermophilic archaeon Sulfolobus solfataricusPenny Worthington
Beadle Center for Genetics, University of Nebraska, Lincoln 68588, USA
J Bacteriol 185:482-8. 2003b>Sulfolobus solfataricus secretes an acid-resistant alpha-amylase (amyA) during growth on starch as the sole carbon and energy source. Synthesis of this activity is subject to catabolite repression...
- Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB proteinIain D Kerr
Centre for Biomolecular Science, St Andrews University, Fife, KY16 9ST, UK
EMBO J 22:2561-70. 2003..We have determined the crystal structure of the SSB protein from the crenarchaeote Sulfolobus solfataricus to 1.26 A...
- Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poletaF Boudsocq
Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human Development, National Institutes of Health, MD 20892 2725, USA
Nucleic Acids Res 29:4607-16. 2001..Searches of the complete genome of the crenarchaeon Sulfolobus solfataricus P2 reveal that it possesses a DinB homolog that has been termed DNA polymerase IV (Dpo4)...
- Complete genome sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain7Y Kawarabayasi
National Institute of Technology and Evaluation, Shibuya, Tokyo, Japan
DNA Res 8:123-40. 2001The complete genomic sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain7 which optimally grows at 80 degrees C, at low pH, and under aerobic conditions, has been determined by the whole genome shotgun method ..
- A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vectorMelanie Jonuscheit
Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstr 10, Germany
Mol Microbiol 48:1241-52. 2003b>Sulfolobus solfataricus has developed into an important model organism for molecular and biochemical studies of hyperthermophilic archaea...
- An abundant DNA binding protein from the hyperthermophilic archaeon Sulfolobus shibatae affects DNA supercoiling in a temperature-dependent fashionH Xue
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People s Republic of China
J Bacteriol 182:3929-33. 2000..DNA binding protein Ssh10b, a member of the Sac10b family, has been purified from the hyperthermophilic archaeon Sulfolobus shibatae. Ssh10b constitutes about 4% of the cellular protein...
- Complete nucleotide sequence of the virus SSV1 of the archaebacterium Sulfolobus shibataeP Palm
Max Planck Institut fur Biochemie, Martinsried, Germany
Virology 185:242-50. 1991The DNA sequence of the Sulfolobus shibatae virus SSV1 is the first complete sequence of an archaebacterial virus genome. The viral DNA is a closed double-stranded DNA circle of 15465 bp...
- Genomic comparison of archaeal conjugative plasmids from SulfolobusBo Greve
Danish Archaea Centre, Institute of Molecular Biology, Copenhagen University, Sølvgade 83H, DK 1307 Copenhagen K, Denmark
Archaea 1:231-9. 2004All of the known self-transmissable plasmids of the Archaea have been found in the genus Sulfolobus. To gain more insight into archaeal conjugative processes, four newly isolated self-transmissable plasmids, pKEF9, pHVE14, pARN3 and pARN4,..
- A novel type of replicative enzyme harbouring ATPase, primase and DNA polymerase activityGeorg Lipps
Department of Biochemistry, University of Bayreuth, Universitatsstrasse 30, Germany
EMBO J 22:2516-25. 2003..The protein ORF904, encoded by the plasmid pRN1 from the thermoacidophile archaeon Sulfolobus islandicus, is a highly compact multifunctional enzyme with ATPase, primase and DNA polymerase activity...
- A novel ligand-binding domain involved in regulation of amino acid metabolism in prokaryotesThijs J G Ettema
Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, NL 6307 CT Wageningen, The Netherlands
J Biol Chem 277:37464-8. 2002..Both domains appear to play analogous roles in controlling key steps in amino acid metabolism at the level of gene expression as well as enzyme activity...
- An atypical topoisomerase II from Archaea with implications for meiotic recombinationA Bergerat
Institut de Genetique et Microbiologie, Universite Paris Sud, CNRS URA 1354, Orsay, France
Nature 386:414-7. 1997..We have cloned and sequenced the genes encoding the A and B subunits of topoisomerase II from the archaeon Sulfolobus shibatae. This enzyme is the first of a new family...
- Solution structure and DNA-binding properties of a thermostable protein from the archaeon Sulfolobus solfataricusH Baumann
Center for Structural Biochemistry, Karolinska Institutet, Novum, Huddinge, Sweden
Nat Struct Biol 1:808-19. 1994The archaeon Sulfolobus solfataricus expresses large amounts of a small basic protein, Sso7d, which was previously identified as a DNA-binding protein possibly involved in compaction of DNA...
- Characterization of the transcriptional activity of the cryptic plasmid pRN1 from Sulfolobus islandicus REN1H1 and regulation of its replication operonSilvia Berkner
Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
J Bacteriol 189:1711-21. 2007The plasmid pRN1 from Sulfolobus islandicus REN1H1 belongs to the crenarchaeal plasmid family pRN...
- An active nonautonomous mobile element in Sulfolobus islandicus REN1H1Silvia Berkner
Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany
J Bacteriol 189:2145-9. 2007In the crenarchaeote Sulfolobus islandicus REN1H1, a mobile element of 321 bp length has been shown to be active. It does not contain terminal inverted repeats and transposes by a replicative mechanism...
- Purification and characterization of a thermostable carboxypeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricusS Colombo
Dipartimento di Fisiologia e Biochimica Generali, Universita di Milano, Italy
Eur J Biochem 206:349-57. 1992A carboxypeptidase was purified to electrophoretic homogeneity from the thermoacidophilic archaebacterium Sulfolobus solfataricus...
- Characterization of the Sulfolobus host-SSV2 virus interactionPatrizia Contursi
Department of Biological Chemistry, Institute of Molecular Biology and Physiology, University of Copenhagen, Copenhagen, Denmark
Extremophiles 10:615-27. 2006The Sulfolobus spindle virus, SSV2, encodes a tyrosine integrase which furthers provirus formation in host chromosomes...
- Sulfolobus solfataricus translation initiation factor 1 stimulates translation initiation complex formationDavid Hasenöhrl
Max F Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter, Dr Bohrgasse 9 4, 1030 Vienna, Austria
RNA 12:674-82. 2006..Here, we show that the archaeal homolog aIF1 has analogous functions. The aIF1 protein of the archaeon Sulfolobus solfataricus is bound to the small ribosomal subunit during translation initiation and accelerates binding of ..
- The genetic element pSSVx of the extremely thermophilic crenarchaeon Sulfolobus is a hybrid between a plasmid and a virusH P Arnold
Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
Mol Microbiol 34:217-26. 1999A new Sulfolobus islandicus strain, REY15/4, harboured both a novel fusellovirus, SSV2, and a small plasmid, pSSVx. The plasmid spread in S...
- Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanismsMarc Graille
Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619 CNRS, Universite Paris Sud, IFR115, F 91405 Orsay, France
Structure 16:360-70. 2008..This structure, combined with a wealth of experimental data gathered on these enzymes, allows us to propose a structural model for the two-gate DNA transfer mechanism...
- Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7Naoto Ohtani
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997 0017, Japan
Nucleic Acids Res 32:5809-19. 2004ST0753, the orthologous gene of Type 1 RNase H found in a thermoacidophilic archaeon, Sulfolobus tokodaii, was analyzed. The recombinant ST0753 protein exhibited RNase H activity in both in vivo and in vitro assays...
- The structure of the NTPase that powers DNA packaging into Sulfolobus turreted icosahedral virus 2Lotta J Happonen
Institute of Biotechnology, University of Helsinki, Helsinki, Finland
J Virol 87:8388-98. 2013..thermophilic NTPase, B204, from the nontailed, membrane-containing, hyperthermoacidophilic archaeal dsDNA virus Sulfolobus turreted icosahedral virus 2...
- Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeonCarole Jaubert
Departement de Microbiologie, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Institut Pasteur, Paris, France
Open Biol 3:130010. 2013The 2 465 177 bp genome of Sulfolobus islandicus LAL14/1, host of the model rudivirus SIRV2, was sequenced. Exhaustive comparative genomic analysis of S. islandicus LAL14/1 and the nine other completely sequenced S...
- Small abundant DNA binding proteins from the thermoacidophilic archaeon Sulfolobus shibatae constrain negative DNA supercoilsV Q Mai
Department of Biology, Pomona College, Claremont, California 91711, USA
J Bacteriol 180:2560-3. 1998Major DNA binding proteins, designated Ssh7, were purified from the thermoacidophilic archaeon Sulfolobus shibatae. The Ssh7 proteins have an apparent molecular mass of 6...
- Structure, function, and targets of the transcriptional regulator SvtR from the hyperthermophilic archaeal virus SIRV1Florence Guillière
Institut Pasteur, Unité de RMN des Biomolécules, CNRS URA 2185, 75015 Paris, France
J Biol Chem 284:22222-37. 2009..SvtR (formerly called gp08) from the rod-shaped virus SIRV1, which infects the hyperthermophilic archaeon Sulfolobus islandicus that thrives at 85 degrees C in hot acidic springs. The protein forms a dimer in solution...
- A novel member of the bacterial-archaeal regulator family is a nonspecific dna-binding protein and induces positive supercoilingA Napoli
Institute of Protein Biochemistry and Enzymology, Consiglio Nazionale delle Ricerche, Via Marconi 10, 80125 Naples, Italy
J Biol Chem 276:10745-52. 2001..We report the identification and characterization of Smj12, a novel protein of Sulfolobus solfataricus, which is homologous to members of the so-called Bacterial-Archaeal family of regulators, found in ..
- Structure of A197 from Sulfolobus turreted icosahedral virus: a crenarchaeal viral glycosyltransferase exhibiting the GT-A foldEric T Larson
Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59715, USA
J Virol 80:7636-44. 2006b>Sulfolobus turreted icosahedral virus (STIV) was the first icosahedral virus characterized from an archaeal host. It infects Sulfolobus species that thrive in the acidic hot springs (pH 2.9 to 3...
- A synthetic arabinose-inducible promoter confers high levels of recombinant protein expression in hyperthermophilic archaeon Sulfolobus islandicusNan Peng
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, People s Republic of China
Appl Environ Microbiol 78:5630-7. 2012..Here we report a versatile vector that confers high levels of protein expression in Sulfolobus islandicus, a hyperthermophilic crenarchaeon...
- An intracellular protease of the crenarchaeon Sulfolobus solfataricus, which has sequence similarity to eukaryotic peptidases of the CD clanAnnamaria Guagliardi
Dipartimento di Chimica Biologica, Universita Federico II di Napoli, Via Mezzocannone 16, 80134 Napoli, Italy
Biochem J 368:357-63. 2002We purified from crude extracts of the hyperthermophilic crenarchaeon Sulfolobus solfataricus a protease that is able to hydrolyse proteins with a pH optimum of 7.5 and a temperature optimum of 70 degrees C...
- Recent advances in genetic analyses of hyperthermophilic archaea and bacteriaK M Noll
Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3125, USA
Arch Microbiol 168:73-80. 1997..Transformation protocols have already been reported for Sulfolobus and Pyrococcus, and plasmid-mediated conjugation was recently found in Sulfolobus...
- A distinctive single-strand DNA-binding protein from the Archaeon Sulfolobus solfataricusCynthia A Haseltine
Division of Biological Sciences, Sections of Microbiology and of Molecular and Cellular Biology, Center for Genetics and Development, University of California Davis, 95616 8665, USA
Mol Microbiol 43:1505-15. 2002..and a zinc-finger motif within a single polypeptide of 645 amino acids, as for Methanococcus jannaschii, the Sulfolobus solfataricus SSB protein (SsoSSB) has a single DNA-binding domain in a polypeptide of just 148 amino acids with ..
- Characterization of Sulfolobus islandicus rod-shaped virus 2 gp19, a single-strand specific endonucleaseAndrew F Gardner
New England Biolabs, Inc, Ipswich, MA 01938, USA
Extremophiles 15:619-24. 2011The hyperthermophilic Sulfolobus islandicus rod-shaped virus 2 (SIRV2) encodes a 25-kDa protein (SIRV2gp19) annotated as a hypothetical protein with sequence homology to the RecB nuclease superfamily...
- The architecture and chemical stability of the archaeal Sulfolobus turreted icosahedral virusReza Khayat
Scripps Research Institute, Department of Molecular Biology, La Jolla, California, USA
J Virol 84:9575-83. 2010..The Sulfolobus turreted icosahedral virus (STIV) is a double-stranded DNA (dsDNA) archaeal virus that contains a host-derived ..
- Genus-specific protein binding to the large clusters of DNA repeats (short regularly spaced repeats) present in Sulfolobus genomesXu Peng
Danish Archaea Centre, Institute of Molecular Biology, University of Copenhagen, Sølvgade 83H, DK 1307 Copenhagen, Denmark
J Bacteriol 185:2410-7. 2003..For the crenarchaeon Sulfolobus solfataricus P2, the repeats in the genome fall mainly into two closely related sequence families that are ..
- A spreadable, non-integrative and high copy number shuttle vector for Sulfolobus solfataricus based on the genetic element pSSVx from Sulfolobus islandicusTiziana Aucelli
Istituto di Biochimica delle Proteine, Via P Castellino 111, 80131 CNR, Napoli, Italy
Nucleic Acids Res 34:e114. 2006The pSSVx genetic element from Sulfolobus islandicus REY15/4 is a hybrid between a plasmid and a fusellovirus, able to be maintained in non-integrative form and to spread when the helper SSV2 virus is present in the cells...
- Thermoadaptation of a mesophilic hygromycin B phosphotransferase by directed evolution in hyperthermophilic Archaea: selection of a stable genetic marker for DNA transfer into Sulfolobus solfataricusR Cannio
Dipartimento di Chimica Organica e Biologica, Universita di Napoli Federico II, Italy
Extremophiles 5:153-9. 2001..Escherichia coli, isolated by directed evolution at 75 degrees C in transformants of a thermophilic strain of Sulfolobus solfataricus, was characterized with respect to its genetic stability in both the original mesophilic and the ..
- Gene expression in archaebacteria: physical mapping of constitutive and UV-inducible transcripts from the Sulfolobus virus-like particle SSV1W D Reiter
Max Planck Institut fur Biochemie, D 8033 Martinsried, Federal Republic of Germany
Mol Gen Genet 209:270-5. 1987The transcription of the genome of the UV-inducible Sulfolobus virus-like particle SSV1 was studied...
- Crystal structure of a thermophilic cytochrome P450 from the archaeon Sulfolobus solfataricusJ K Yano
Department of Molecular Biology and Biochemistry and Program in Macromolecular Structure, University of California, Irvine, California 92697 3900, USA
J Biol Chem 275:31086-92. 2000The structure of the first P450 identified in Archaea, CYP119 from Sulfolobus solfataricus, has been solved in two different crystal forms that differ by the ligand (imidazole or 4-phenylimidazole) coordinated to the heme iron...
- Three conformations of an archaeal chaperonin, TF55 from Sulfolobus shibataeG Schoehn
Department of Crystallography, Birkbeck College, Malet St, London, WC1E 7HX, UK
J Mol Biol 296:813-9. 2000..Using electron cryo-microscopy and three-dimensional reconstruction, we describe three conformations of a group II chaperonin, including an asymmetric, bullet-shaped form, revealing the range of domain movements in this subfamily...
- A unique virus release mechanism in the ArchaeaAriane Bize
Biologie Moléculaire du Gène chez les Extrêmophiles, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
Proc Natl Acad Sci U S A 106:11306-11. 2009..Here, we demonstrate that the virions of the archaeal Sulfolobus islandicus rod-shaped virus 2 (SIRV2) are released from the host cell through a mechanism, involving the ..
- Evolutionary rates and gene dispensability associate with replication timing in the archaeon Sulfolobus islandicusKenneth M Flynn
Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, USA
Genome Biol Evol 2:859-69. 2010..To test this hypothesis, six archaeal genomes from the genus Sulfolobus containing three origins of replication were selected, conserved orthologs were identified, and the evolutionary ..
- Glucose transport in the extremely thermoacidophilic Sulfolobus solfataricus involves a high-affinity membrane-integrated binding proteinS V Albers
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands
J Bacteriol 181:4285-91. 1999The archaeon Sulfolobus solfataricus grows optimally at 80 degrees C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose...
- Stability of mRNA in the hyperthermophilic archaeon Sulfolobus solfataricusElisabetta Bini
George Beadle Center for Genetics, University of Nebraska Lincoln, 68588 0666, USA
RNA 8:1129-36. 2002..Because archaea are generally insensitive to antibiotics, examination of mRNA stability in the hyperthermophile, Sulfolobus solfataricus, required the identification of transcription inhibitors for half-life determinations...
- Mechanism of DNA polymerization catalyzed by Sulfolobus solfataricus P2 DNA polymerase IVKevin A Fiala
Department of Biochemistry, Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210, USA
Biochemistry 43:2116-25. 2004The kinetic mechanism of DNA polymerization catalyzed by Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) is resolved by pre-steady-state kinetic analysis of single-nucleotide (dTTP) incorporation into a DNA 21/41-mer...
- Three replication origins in Sulfolobus species: synchronous initiation of chromosome replication and asynchronous terminationMagnus Lundgren
Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University, Norbyvagen 18C, SE 752 36 Uppsala, Sweden
Proc Natl Acad Sci U S A 101:7046-51. 2004Chromosome replication origins were mapped in vivo in the two hyperthermophilic archaea, Sulfolobus acidocaldarius and Sulfolobus solfataricus, by using microarray-based marker frequency analysis...
- Structure of Alba: an archaeal chromatin protein modulated by acetylationB N Wardleworth
Centre for Biomolecular Science, St Andrews University, North Haugh, St Andrews, Fife KY16 9ST, UK
EMBO J 21:4654-62. 2002..We present the crystal structure of Alba from Sulfolobus solfataricus at 2.6 A resolution (PDB code 1h0x)...
- Aggregation of the Acylphosphatase from Sulfolobus solfataricus: the folded and partially unfolded states can both be precursors for amyloid formationGeorgia Plakoutsi
Dipartimento di Scienze Biochimiche, Universita di Firenze, Viale Morgagni 50, 50134 Firenze, Italy
J Biol Chem 279:14111-9. 2004..In this study, we used the acylphosphatase from the hyperthermophilic Archaea Sulfolobus solfataricus (Sso AcP) to investigate the mechanism of aggregation under conditions in which the protein ..
- Cell cycle characteristics of thermophilic archaeaR Bernander
Department of Microbiology, Biomedical Center, Uppsala University, Sweden
J Bacteriol 179:4963-9. 1997..Exponentially growing cells of the thermophilic archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius were analyzed by flow cytometry, and several unusual cell cycle ..
- Unmarked gene deletion and host-vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicusLing Deng
Huazhong Agricultural University, Wuhan, People s Republic of China
Extremophiles 13:735-46. 2009b>Sulfolobus islandicus is being used as a model for studying archaeal biology, geo-biology and evolution. However, no genetic system is available for this organism. To produce an S...
- A role for the ESCRT system in cell division in archaeaRachel Y Samson
Medical Research Council MRC Cancer Cell Unit, Hills Road, Cambridge CB2 0XZ, UK
Science 322:1710-3. 2008..However, a number of hyperthermophilic members of the Kingdom Crenarchaea, including members of the Sulfolobus genus, encode homologs of the eukaryotic endosomal sorting system components Vps4 and ESCRT-III (endosomal ..
- Purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bondsG Cacciapuoti
Institute of Biochemistry of Macromolecules, Faculty of Medicine, Second University of Naples, Italy
J Biol Chem 269:24762-9. 19945'-Methylthioadenosine phosphorylase from Sulfolobus solfataricus, a thermoacidophilic archaeon optimally growing at 87 degrees C, has been purified to homogeneity. Reducing agents are not required for catalytic activity...
- An error-prone family Y DNA polymerase (DinB homolog from Sulfolobus solfataricus) uses a 'steric gate' residue for discrimination against ribonucleotidesAngela M DeLucia
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
Nucleic Acids Res 31:4129-37. 2003..Nevertheless, ribonucleotide discrimination by the DinB homolog (Dbh) DNA polymerase of Sulfolobus solfataricus is as stringent as in other polymerases...
- Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricusNicholas P Robinson
Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, United Kingdom
Cell 116:25-38. 2004..identification of two active origins of replication in the single chromosome of the hyperthermophilic archaeon Sulfolobus solfataricus...
- Relationships between fuselloviruses infecting the extremely thermophilic archaeon Sulfolobus: SSV1 and SSV2Kenneth M Stedman
Max Planck Institut fur Biochemie, Am Klopferspitz 18a, 82152 Martinsried, Germany
Res Microbiol 154:295-302. 2003The fusellovirus SSV2 from an Icelandic Sulfolobus strain was isolated, characterized and its complete genomic sequence determined...
- Ferrous iron- and sulfur-induced genes in Sulfolobus metallicusStephan Bathe
Department of Biological Sciences, University of Warwick, Coventry, UK
Appl Environ Microbiol 73:2491-7. 2007Genes of Sulfolobus metallicus that appeared to be upregulated in relation to growth on either ferrous iron or sulfur were identified using subtractive hybridization of cDNAs...
- Archaeal integrases and mechanisms of gene captureQ She
Danish Archaea Centre, Institute of Molecular Biology, Copenhagen University, Sølvgade 83H, DK 1307 Copenhagen K, Denmark
Biochem Soc Trans 32:222-6. 2004..It has been demonstrated that SSV (Sulfolobus spindle virus) viruses, carrying an SSV-type integrase gene, and conjugative plasmids, carrying a pNOB8-type ..
- Comparative study of the extracellular proteome of Sulfolobus species reveals limited secretionAlbert F Ellen
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
Extremophiles 14:87-98. 2010..A proteomic inventory and comparison of the growth medium proteins in three hyperthermoacidophiles, i.e., Sulfolobus solfataricus, S. acidocaldarius and S...
- Thermal and conformational stability of Ssh10b protein from archaeon Sulfolobus shibattaeSu Xu
National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing 100101, China
Biochem J 382:433-40. 2004..The small DeltaC(p) of Ssh10b appears to result mainly from the presence of compactness in the denatured state...
- Development of a genetic system for the archaeal virus Sulfolobus turreted icosahedral virus (STIV)Jennifer Fulton Wirth
Thermal Biology Institute, Montana State University, Bozeman, MT, USA
Virology 415:6-11. 2011..We describe the construction of an infectious clone for the archaeal virus Sulfolobus turreted icosahedral virus (STIV). STIV was isolated from a high temperature (82°C) acidic (pH 2...
- The genome of the archaeal virus SIRV1 has features in common with genomes of eukaryal virusesH Blum
, Feodor-Lynen-Str. 25, D-81377 Munich, Germany
Virology 281:6-9. 2001The virus SIRV1 of the extremely thermophilic archaeon Sulfolobus has a double-stranded DNA genome similar in architecture to the genomes of eukaryal viruses of the families Poxviridae, Pycodnaviridae, and Asfarviridae: the two strands of ..
- Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Sulfolobus solfataricus via chemical rescue of an inactive mutantBeatrice Cobucci-Ponzano
Institute of Protein Biochemistry, Consiglio Nazionale delle Ricerche, Via P Castellino 111, 80131, Naples, Italy
Biochemistry 42:9525-31. 2003..mutation in the region of overlap between the ORFs SSO11867 and SSO3060 of the hyperthermophilic Archaeon Sulfolobus solfataricus [Cobucci-Ponzano et al. J. Biol. Chem. (2003) 278, 14622-14631]...
- Crystallization and preliminary X-ray diffraction studies of Hje, a HolliDay junction resolving enzyme from Sulfolobus solfataricusClaire L Middleton
Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland
Acta Crystallogr D Biol Crystallogr 59:171-3. 2003HolliDay junction endonuclease (Hje) from Sulfolobus solfataricus is a resolving enzyme involved in cleaving specific sites on either side of recombinant four-way HolliDay junctions. The HJE gene from S...
- The structure of a thermophilic archaeal virus shows a double-stranded DNA viral capsid type that spans all domains of lifeGeorge Rice
Thermal Biology Institute and Department of Microbiology, Montana State University, Bozeman, MT 59717, USA
Proc Natl Acad Sci U S A 101:7716-20. 2004....
- Development of a simvastatin selection marker for a hyperthermophilic acidophile, Sulfolobus islandicusTao Zheng
State Key Laboratory of Microbial Technology, Shandong University, Jinan, People s Republic of China
Appl Environ Microbiol 78:568-74. 2012We report here a novel selectable marker for the hyperthermophilic crenarchaeon Sulfolobus islandicus...
- A broadly applicable gene knockout system for the thermoacidophilic archaeon Sulfolobus islandicus based on simvastatin selectionChangyi Zhang
Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, IL, USA
Microbiology 158:1513-22. 2012b>Sulfolobus species have been developed as excellent model organisms to address fundamental questions of archaeal biology...
- The putative DNA-binding protein Sto12a from the thermoacidophilic archaeon Sulfolobus tokodaii contains intrachain and interchain disulfide bondsAkeo Shinkai
RIKEN SPring 8 Center, Harima Institute, 1 1 1 Kouto, Sayo, Hyogo 679 5148, Japan
J Mol Biol 372:1293-304. 2007The Sto12a protein, from the thermoacidophilic archaeon Sulfolobus tokodaii, has been identified as a small putative DNA-binding protein...
- Archaeal RNA polymerase: the influence of the protruding stalk in crystal packing and preliminary biophysical analysis of the Rpo13 subunitMagdalena Wojtas
Structural Biology Unit, CIC bioGUNE, CIBERehd, 48160 Derio, Spain
Biochem Soc Trans 39:25-30. 2011We review recent results on the complete structure of the archaeal RNAP (RNA polymerase) enzyme of Sulfolobus shibatae...
- Sulfolobus islandicus: a model system for evolutionary genomicsChangyi Zhang
Department of Microbiology, University of Illinois at Urbana Champaign, 601 S Goodwin Ave, Urbana, IL 61801, USA
Biochem Soc Trans 41:458-62. 2013b>Sulfolobus islandicus has been developed as a model system for combining approaches of evolutionary and molecular biology in Archaea...
- Structural study of a single-point mutant of Sulfolobus solfataricus alcohol dehydrogenase with enhanced activityLuciana Esposito
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 6 8, I 80134 Naples, Italy
FEBS Lett 539:14-8. 2003Alcohol dehydrogenase from Sulfolobus solfataricus (SsADH) is the only enzyme from Archaea among the structurally studied members of the medium-chain ADH family described so far...
- Homology modeling, molecular dynamics simulations, and analysis of CYP119, a P450 enzyme from extreme acidothermophilic archaeon Sulfolobus solfataricusY T Chang
Molecular Research Institute, 2495 Old Middlefield Way, Mountain View, California 94043 2316, USA
Biochemistry 39:2484-98. 2000The recent characterization of a thermophilic and barophilic CYP119 from Sulfolobus solfataricus offers a new opportunity to identify the origin of its stability by comparing it with mesophilic P450s with known structures...
- SDS-resistant active and thermostable dimers are obtained from the dissociation of homotetrameric beta-glycosidase from hyperthermophilic Sulfolobus solfataricus in SDS. Stabilizing role of the A-C intermonomeric interfaceFabrizio Gentile
Istituto di Endocrinologia e Oncologia Sperimentale del CNR and Dipartimento di Biologia e Patologia Cellulare e Molecolare, Universita Federico II, Via Pansini 5, 80131 Napoli, Italy
J Biol Chem 277:44050-60. 2002..Previous work with homotetrameric beta-glycosidase from hyperthermophilic Sulfolobus solfataricus (M(r) 226,760) has shown that addition of 0.05-0...
- Peptidyl-tRNA hydrolase from Sulfolobus solfataricusMichel Fromant
Laboratoire de Biochimie, Unité Mixte de Recherche 7654, CNRS Ecole Polytechnique, 91128 Palaiseau Cedex, France
Nucleic Acids Res 31:3227-35. 2003..of liberating functional tRNA(Lys) from Escherichia coli diacetyl-lysyl-tRNA(Lys) was purified from the archae Sulfolobus solfataricus. Contrasting with the specificity of peptidyl- tRNA hydrolase (PTH) from E.coli, the S...
- Sulerythrin, the smallest member of the rubrerythrin family, from a strictly aerobic and thermoacidophilic archaeon, Sulfolobus tokodaii strain 7Takayoshi Wakagi
Department of Biotechnology, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, 113 8657, Japan
FEMS Microbiol Lett 222:33-7. 2003A protein corresponding to the N-terminal domain of rubrerythrin was isolated from a strictly aerobic archaeon, Sulfolobus tokodaii strain 7. The molecular mass was found to be 15...
- Crystal structure of Sulfolobus tokodaii Sua5 complexed with L-threonine and AMPPNPMitsuo Kuratani
RIKEN Systems and Structural Biology Center, Yokohama 230 0045, Japan
Proteins 79:2065-75. 2011..In this study, we analyzed the L-threonine-binding mode of Sua5 from the archaeon Sulfolobus tokodaii. Isothermal titration calorimetry measurements revealed that S...
- Microbial Diversity in Mechanisms of Disulfide Bond Formation and ReductionDana Boyd; Fiscal Year: 2013..coli and the archaeon Sulfolobus solfataricus...
- X-ray crystallographic studies of multi-subunit nucleic acid polymerasesKatsuhiko Murakami; Fiscal Year: 2013..We recently reported the first X-ray crystal structure of the archaeal RNA polymerase from Sulfolobus solfataricus at 3.4 E resolution...
- Polymerase Interactions with Carcinogen-modified DNAF Peter Guengerich; Fiscal Year: 2013..One focus will be biochemical and in vivo studies with the DNA polymerases of a model organism, the crenarcheon Sulfolobus solfataricus, and approaches will be extended to human Y- family polymerases...
- Biochemical and structural characterization of self-assembling viral particlesREBECCA ETHEL TAUROG; Fiscal Year: 2010The Sulfolobus islandicus rod-shaped virus (SIRV) is a fibrous virus that infects thermophilic, acidophilic Archaea found in thermal vents and hot springs around the world...
- Structural Characterization of Archaeal Virus Assembly and Host InteractionsChi Yu Fu; Fiscal Year: 2012..We have been able to apply whole cell electron cryotomography (ECT) to follow the assembly and maturation of Sulfolobus turreted icosahedral virus (STIV) in a life-like state in intact archaeon Sulfolobus cells...
- Structural and Mechanistic Studies of Error-Prone PolymerasesJanice D Pata; Fiscal Year: 2012..We are using the DinB homolog (Dbh) from Sulfolobus acidocaldarius as a model for the DinB class of DNA polymerases...
- Data Mining Assisted Discovery of Novel OrganophosphorouRobert Turner; Fiscal Year: 2005..has already been used to discover two novel OPH's from the thermophiles Geobacillus stearothermophilus and Sulfolobus solfataricus...
- Biophysical studies of a Sulfolobus icosahedral virusReza Khayat; Fiscal Year: 2007The Sulfolobus Turreted Icosahedral virus (STIV) is a lytic hyperthermophile virus that infects members of the Sulfolobus family within the kingdom Crenarchaeota of the domain Archaea...
- INTERACTIONS OF SEQUENCE-GENERAL DNA-BINDING PROTEINSCharles Miller; Fiscal Year: 2001..In particular, chromosomal proteins from archaeabacteria (Sulfolobus) and bacteria (E...
- THE CCA-ADDING ENZYME (tRNA NUCLEOTIDYL TRANSFERASE)Alan Weiner; Fiscal Year: 2006..of the active site, scrunching pocket, and tRNA-binding regions of four different enzymes: the archaeal class I Sulfolobus shibatae CCA-adding enzyme (Aim 1), the eubacterial class II Bacillus stearothermophilus CCA-adding enzyme (Aim ..
- MOLECULAR MECHANISM OF HMG COA REDUCTASECynthia Stauffacher; Fiscal Year: 2002..reaction which can be examined by X- ray crystallographic techniques, and to extend these studies to the Sulfolobus and Syrian hamster enzymes...
- GENOMIC ANALYSIS OF LAMBDOID, TB & OTHER BACTERIOPHAGESRoger Hendrix; Fiscal Year: 2007..abstract_text> ..
- DNA RADICAL KINETICSMartin Newcomb; Fiscal Year: 2001....
- MECHANISTIC PROBES FOR ENZYME-CATALYZED OXIDATIONSMARTIN E NEWCOMB; Fiscal Year: 2010..A better understanding of the fundamental nature of the reactive species formed in the enzymes and the mechanisms of their reactions is expected to lead to lead ultimately to health-related advances in disease treatment and drug design. ..