entamoeba histolytica

Summary

Summary: A species of parasitic protozoa causing ENTAMOEBIASIS and amebic dysentery (DYSENTERY, AMEBIC). Characteristics include a single nucleus containing a small central karyosome and peripheral chromatin that is finely and regularly beaded.

Top Publications

  1. ncbi Amoebiasis
    Samuel L Stanley
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO, St Louis, MO 63110, USA
    Lancet 361:1025-34. 2003
  2. ncbi Amebiasis
    Rashidul Haque
    International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh
    N Engl J Med 348:1565-73. 2003
  3. ncbi Phosphatidylinositol-phosphates mediate cytoskeletal reorganization during phagocytosis via a unique modular protein consisting of RhoGEF/DH and FYVE domains in the parasitic protozoon Entamoeba histolytica
    Kumiko Nakada-Tsukui
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
    Cell Microbiol 11:1471-91. 2009
  4. pmc New assembly, reannotation and analysis of the Entamoeba histolytica genome reveal new genomic features and protein content information
    Hernan A Lorenzi
    J Craig Venter Institute, Rockville, Maryland, United States of America
    PLoS Negl Trop Dis 4:e716. 2010
  5. ncbi The bittersweet interface of parasite and host: lectin-carbohydrate interactions during human invasion by the parasite Entamoeba histolytica
    William A Petri
    Division of Infectious Diseases, University of Virginia, MR4 Bldg Room 2115, Lane Road, Charlottesville 22908 1340, USA
    Annu Rev Microbiol 56:39-64. 2002
  6. pmc An ex-vivo human intestinal model to study Entamoeba histolytica pathogenesis
    Devendra Bansal
    Institut Pasteur, Unité de Biologie Cellulaire du Parasitisme, Paris, France
    PLoS Negl Trop Dis 3:e551. 2009
  7. ncbi The immunopathogenesis of Entamoeba histolytica
    Leanne Mortimer
    Faculty of Medicine, Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Dr NW, Calgary, Alberta, Canada
    Exp Parasitol 126:366-80. 2010
  8. pmc Entamoeba histolytica cysteine proteases cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel
    Martin E Lidell
    Department of Medical Biochemistry, Goteborg University, Medicinaregatan 9A, S 413 90 Gothenburg, Sweden
    Proc Natl Acad Sci U S A 103:9298-303. 2006
  9. pmc Tissue destruction and invasion by Entamoeba histolytica
    Katherine S Ralston
    Department of Medicine, University of Virginia Health Sciences Center, Charlottesville, VA, USA
    Trends Parasitol 27:254-63. 2011
  10. ncbi A C2 domain protein kinase initiates phagocytosis in the protozoan parasite Entamoeba histolytica
    - Somlata
    School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
    Nat Commun 2:230. 2011

Detail Information

Publications323 found, 100 shown here

  1. ncbi Amoebiasis
    Samuel L Stanley
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO, St Louis, MO 63110, USA
    Lancet 361:1025-34. 2003
    ..The causative protozoan parasite, Entamoeba histolytica, is a potent pathogen...
  2. ncbi Amebiasis
    Rashidul Haque
    International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh
    N Engl J Med 348:1565-73. 2003
  3. ncbi Phosphatidylinositol-phosphates mediate cytoskeletal reorganization during phagocytosis via a unique modular protein consisting of RhoGEF/DH and FYVE domains in the parasitic protozoon Entamoeba histolytica
    Kumiko Nakada-Tsukui
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
    Cell Microbiol 11:1471-91. 2009
    ..PtdIns(3)P] and putative PtdIns-P-binding proteins during phagocytosis in the enteric protozoan parasite Entamoeba histolytica. It was previously shown that phagocytosis in E...
  4. pmc New assembly, reannotation and analysis of the Entamoeba histolytica genome reveal new genomic features and protein content information
    Hernan A Lorenzi
    J Craig Venter Institute, Rockville, Maryland, United States of America
    PLoS Negl Trop Dis 4:e716. 2010
    ..For this reason, after five years from the initial submission of the Entamoeba histolytica draft genome publication, we have re-examined the original 23 Mb assembly and the annotation of the ..
  5. ncbi The bittersweet interface of parasite and host: lectin-carbohydrate interactions during human invasion by the parasite Entamoeba histolytica
    William A Petri
    Division of Infectious Diseases, University of Virginia, MR4 Bldg Room 2115, Lane Road, Charlottesville 22908 1340, USA
    Annu Rev Microbiol 56:39-64. 2002
    b>Entamoeba histolytica, as its name suggests, is an enteric parasite with a remarkable ability to lyse host tissues. However, the interaction of the parasite with the host is more complex than solely destruction and invasion...
  6. pmc An ex-vivo human intestinal model to study Entamoeba histolytica pathogenesis
    Devendra Bansal
    Institut Pasteur, Unité de Biologie Cellulaire du Parasitisme, Paris, France
    PLoS Negl Trop Dis 3:e551. 2009
    ..a human intestinal infection affecting 50 million people every year) is caused by the protozoan parasite Entamoeba histolytica. To study the molecular mechanisms underlying human colon invasion by E...
  7. ncbi The immunopathogenesis of Entamoeba histolytica
    Leanne Mortimer
    Faculty of Medicine, Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, 3330 Hospital Dr NW, Calgary, Alberta, Canada
    Exp Parasitol 126:366-80. 2010
    Amebiasis is the disease caused by the enteric dwelling protozoan parasite Entamoeba histolytica. The WHO considers amebiasis as one of the major health problems in developing countries; it is surpassed by only malaria and ..
  8. pmc Entamoeba histolytica cysteine proteases cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel
    Martin E Lidell
    Department of Medical Biochemistry, Goteborg University, Medicinaregatan 9A, S 413 90 Gothenburg, Sweden
    Proc Natl Acad Sci U S A 103:9298-303. 2006
    In order for the protozoan parasite Entamoeba histolytica (E.h...
  9. pmc Tissue destruction and invasion by Entamoeba histolytica
    Katherine S Ralston
    Department of Medicine, University of Virginia Health Sciences Center, Charlottesville, VA, USA
    Trends Parasitol 27:254-63. 2011
    b>Entamoeba histolytica is the causative agent of amebiasis, a disease that is a major source of morbidity and mortality in the developing world...
  10. ncbi A C2 domain protein kinase initiates phagocytosis in the protozoan parasite Entamoeba histolytica
    - Somlata
    School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
    Nat Commun 2:230. 2011
    ..In the protozoan parasite Entamoeba histolytica, it has an essential role in survival and pathogenesis...
  11. pmc Leptin signaling in intestinal epithelium mediates resistance to enteric infection by Entamoeba histolytica
    X Guo
    Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA
    Mucosal Immunol 4:294-303. 2011
    ..Previous observation that a genetic polymorphism in the leptin receptor affected susceptibility to Entamoeba histolytica infection led to the hypothesis that leptin signaling has a protective role during intestinal amebic ..
  12. pmc Nucleus-localized antisense small RNAs with 5'-polyphosphate termini regulate long term transcriptional gene silencing in Entamoeba histolytica G3 strain
    Hanbang Zhang
    Division of Infectious Diseases, Department of Internal Medicine, Stanford University School of Medicine, Stanford, California 94305 5107, USA
    J Biol Chem 286:44467-79. 2011
    In the deep-branching eukaryotic parasite Entamoeba histolytica, transcriptional gene silencing (TGS) of the Amoebapore A gene (ap-a) in the G3 strain has been reported with subsequent development of this parasite strain for gene ..
  13. pmc Global analysis of gene expression in response to L-Cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica
    Afzal Husain
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    BMC Genomics 12:275. 2011
    b>Entamoeba histolytica, an enteric protozoan parasite, causes amebic colitis and extra intestinal abscesses in millions of inhabitants of endemic areas. E...
  14. pmc Downregulation of an Entamoeba histolytica rhomboid protease reveals roles in regulating parasite adhesion and phagocytosis
    Leigh A Baxt
    Department of Microbiology and Immunology, Stanford University School of Medicine, S 143 Grant Building, 300 Pasteur Drive, Stanford, CA 94305, USA
    Eukaryot Cell 9:1283-93. 2010
    b>Entamoeba histolytica is a deep-branching eukaryotic pathogen. Rhomboid proteases are intramembrane serine proteases, which cleave transmembrane proteins in, or in close proximity to, their transmembrane domain...
  15. pmc Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR
    Jaco J Verweij
    Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    J Clin Microbiol 42:1220-3. 2004
    b>Entamoeba histolytica, Giardia lamblia, and Cryptosporidium are three of the most important diarrhea-causing parasitic protozoa...
  16. pmc Prospective case-control study of the association between common enteric protozoal parasites and diarrhea in Bangladesh
    Rashidul Haque
    International Centre for Diarrhoeal Disease Research, Bangladesh
    Clin Infect Dis 48:1191-7. 2009
    ..was to determine, by use of modern diagnostic methods, the proportion of diarrhea cases in Bangladesh attributable to Cryptosporidium hominis, Cryptosporidium parvum, Entamoeba histolytica, and Giardia lamblia assemblages A and B.
  17. pmc Localization and targeting of an unusual pyridine nucleotide transhydrogenase in Entamoeba histolytica
    Mohammad Abu Yousuf
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo, Japan
    Eukaryot Cell 9:926-33. 2010
    ..mitochondrion-related organelle, the mitosome, in the anaerobic/microaerophilic protozoan parasite Entamoeba histolytica based on the potential mitochondrion-targeting signal...
  18. ncbi The lysine- and glutamic acid-rich protein KERP1 plays a role in Entamoeba histolytica liver abscess pathogenesis
    Julien Santi-Rocca
    Institut Pasteur, Unité Biologie Cellulaire du Parasitisme, Paris, F 75015, France, and INSERM U786, Paris, F 75015, France
    Cell Microbiol 10:202-17. 2008
    The parasite Entamoeba histolytica colonizes the large bowel where it may persist as an asymptomatic luminal gut infection, which changes to virulence...
  19. pmc Evaluation of the Triage Micro Parasite Panel for detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum in patient stool specimens
    S E Sharp
    Department of Pathology and Laboratory Medicine, Mount Sinai Medical Center, Miami Beach, Florida 33140, USA
    J Clin Microbiol 39:332-4. 2001
    ..Seven specimens were positive for Giardia lamblia, four were positive for Entamoeba histolytica/E. dispar, and three were positive for Cryptosporidium parvum as determined by both methods...
  20. ncbi Extracellular matrix-induced signaling in Entamoeba histolytica: its role in invasiveness
    I Meza
    Departamento de Biomedicina Molecular, CINVESTAV del IPN, Mexico, D F, Mexico
    Parasitol Today 16:23-8. 2000
    b>Entamoeba histolytica trophozoites can invade the mucosa of the host large intestine to cause disease...
  21. ncbi Epidemiologic and clinical characteristics of acute diarrhea with emphasis on Entamoeba histolytica infections in preschool children in an urban slum of Dhaka, Bangladesh
    Rashidul Haque
    Centre for Health and Population Research, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
    Am J Trop Med Hyg 69:398-405. 2003
    ..use of improved diagnostic tests for amebiasis enabled for the first time analysis of the contribution of Entamoeba histolytica to total diarrheal illness in this community setting. The average incidence rate of diarrhea was 1...
  22. pmc Longitudinal study of intestinal Entamoeba histolytica infections in asymptomatic adult carriers
    Joerg Blessmann
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 41:4745-50. 2003
    To gain insight into the dynamics of intestinal Entamoeba histolytica infection, a longitudinal study was performed over an observation period of 15 months with a group of 383 randomly selected adult individuals (mean age, 38...
  23. pmc Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica
    Fumika Mi-ichi
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
    PLoS Negl Trop Dis 5:e1263. 2011
    ..We have previously shown that mitosomes of the anaerobic/microaerophilic intestinal protozoan parasite Entamoeba histolytica have uniquely evolved and compartmentalized a sulfate activation pathway...
  24. ncbi The use of real-time PCR to identify Entamoeba histolytica and E. dispar infections in prisoners and primary-school children in Ethiopia
    A Kebede
    Ethiopian Health and Nutrition Research Institute, PO Box 1242, Addis Ababa, Ethiopia
    Ann Trop Med Parasitol 98:43-8. 2004
    ..This finding is consistent with the conclusion of a previous, smaller investigation: that many amoebic infections in Ethiopia are incorrectly attributed to E. histolytica and then treated, unnecessarily, with amoebicidal drugs...
  25. ncbi Broad early immune response of porcine epithelial jejunal IPI-2I cells to Entamoeba histolytica
    Francois Meurens
    Institut National de la Recherche Agronomique INRA Tours, UR1282, Infectiologie Animale et Sante Publique, Nouzilly F 37380, France
    Mol Immunol 46:927-36. 2009
    ..This will focus future efforts on delineating the molecular and cellular mechanisms of other cell partners by the way of in vivo infection of swine...
  26. pmc A retromerlike complex is a novel Rab7 effector that is involved in the transport of the virulence factor cysteine protease in the enteric protozoan parasite Entamoeba histolytica
    Kumiko Nakada-Tsukui
    Department of Parasitology, Gunma University Graduate School of Medicine, Maebashi 371 8511, Japan
    Mol Biol Cell 16:5294-303. 2005
    Vesicular trafficking plays an important role in a virulence mechanism of the enteric protozoan parasite Entamoeba histolytica as secreted and lysosomal cysteine protease (CP) contributes to both cytolysis of tissues and degradation of ..
  27. ncbi Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin
    Paul H Davis
    Department of Medicine, Washington University School of Medicine, St Louis, MO, USA
    Mol Biochem Parasitol 151:118-28. 2007
    ..histolytica HM-1:IMSS genome. We find extensive differences between the two strains, including distinct patterns of gene expression of cysteine proteinases, AIG family members, and lectin light chains...
  28. pmc Serum-dependent selective expression of EhTMKB1-9, a member of Entamoeba histolytica B1 family of transmembrane kinases
    Shiteshu Shrimal
    School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
    PLoS Pathog 6:e1000929. 2010
    b>Entamoeba histolytica transmembrane kinases (EhTMKs) can be grouped into six distinct families on the basis of motifs and sequences. Analysis of the E...
  29. ncbi A study on the usefulness of Techlab Entamoeba histolytica II antigen detection ELISA in the diagnosis of amoebic liver abscess (ALA) at Hospital Universiti Sains Malaysia (HUSM), Kelantan, Malaysia
    M Zeehaida
    Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
    Trop Biomed 25:209-16. 2008
    ..Sains Malaysia (HUSM), Kelantan employs an indirect haemagglutination assay (IHA) which detects anti-Entamoeba histolytica antibodies in patients' serum samples...
  30. pmc A proteomic and cellular analysis of uropods in the pathogen Entamoeba histolytica
    Jacques Marquay Markiewicz
    Institut Pasteur, Unité Biologie Cellulaire du Parasitisme, Paris, France
    PLoS Negl Trop Dis 5:e1002. 2011
    Exposure of Entamoeba histolytica to specific ligands induces cell polarization via the activation of signalling pathways and cytoskeletal elements...
  31. pmc Host-microbe interactions and defense mechanisms in the development of amoebic liver abscesses
    Julien Santi-Rocca
    Unité Biologie Cellulaire du Parasitisme, Institut Pasteur, Paris Cedex 15, France
    Clin Microbiol Rev 22:65-75, Table of Contents. 2009
    Amoebiasis by Entamoeba histolytica is a major public health problem in developing countries and leads to several thousand deaths per year...
  32. pmc New insights into Entamoeba histolytica pathogenesis
    Leigh A Baxt
    Department of Microbiology and Immunology, Stanford University, Stanford, California, USA
    Curr Opin Infect Dis 21:489-94. 2008
    b>Entamoeba histolytica is an important global pathogen and a leading cause of parasitic death worldwide. This article summarizes significant research findings over the last year.
  33. ncbi EhPAK, a member of the p21-activated kinase family, is involved in the control of Entamoeba histolytica migration and phagocytosis
    Elisabeth Labruyère
    Unité de Biologie Cellulaire du Parasitisme, INSERM U389, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Cell Sci 116:61-71. 2003
    b>Entamoeba histolytica migration is essential for the development of amoebiasis, a human disease characterised by invasion and destruction of tissues...
  34. pmc Members of the Entamoeba histolytica transmembrane kinase family play non-redundant roles in growth and phagocytosis
    Sarah N Buss
    Department of Microbiology, University of Virginia, Charlottesville, VA 22908 1340, USA
    Int J Parasitol 40:833-43. 2010
    b>Entamoeba histolytica contains a large and novel family of transmembrane kinases (TMKs). The expression patterns of the E...
  35. pmc Transcriptional silencing of multiple genes in trophozoites of Entamoeba histolytica
    Rivka Bracha
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
    PLoS Pathog 2:e48. 2006
    In a previous work we described the transcriptional silencing of the amoebapore A (AP-A) gene (Ehap-a) of Entamoeba histolytica strain HM-1:IMSS...
  36. pmc Prevalence and spatial distribution of Entamoeba histolytica/dispar and Giardia lamblia among schoolchildren in Agboville area (Côte d'Ivoire)
    Mamadou Ouattara
    Biosciences Université d Abidjan Cocody, Abidjan, Cote d Ivoire
    PLoS Negl Trop Dis 4:e574. 2010
    ..comprehension of the epidemiology of the intestinal protozoa, prevalence, and spatial distribution of Entamoeba histolytica/dispar and Giardia lamblia, and their association with drinking water supplies, were determined in the ..
  37. ncbi Identification of an avirulent Entamoeba histolytica strain with unique tRNA-linked short tandem repeat markers
    Aleyla Escueta-de Cadiz
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Parasitol Int 59:75-81. 2010
    Highly polymorphic, non-coding short tandem repeats (STR) are scattered between the tRNA genes in Entamoeba histolytica in a unique tandemly arrayed organization. STR markers that correlate with the virulence of individual E...
  38. pmc A new nuclear function of the Entamoeba histolytica glycolytic enzyme enolase: the metabolic regulation of cytosine-5 methyltransferase 2 (Dnmt2) activity
    Ayala Tovy
    Department of Molecular Microbiology, The Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel
    PLoS Pathog 6:e1000775. 2010
    ..PEP), was shown to have both a cytoplasmatic and a nuclear localization in the parasite Entamoeba histolytica. We discovered that enolase acts as a Dnmt2 inhibitor...
  39. ncbi High prevalence rate of Entamoeba histolytica asymptomatic infection in a rural Mexican community
    Fernando Ramos
    Department of Experimental Medicine, School of Medicine, National Autonomous University of Mexico, Mexico Distrito Federal, Mexico
    Am J Trop Med Hyg 73:87-91. 2005
    The frequency of Entamoeba histolytica and Entamoeba dispar infection was analyzed in a rural community in the state of Morelos, Mexico, using polymerase chain reaction (PCR)...
  40. pmc The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica
    Pavel Dolezal
    Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria, Australia
    PLoS Pathog 6:e1000812. 2010
    ..In the case of Entamoeba histolytica, tiny mitochondria known as mitosomes have undergone extreme reduction...
  41. pmc RNA interference in Entamoeba histolytica: implications for parasite biology and gene silencing
    Hanbang Zhang
    Stanford University School of Medicine, S 143 Grant Building, 300 Pasteur Drive, Stanford, CA 94305, USA
    Future Microbiol 6:103-17. 2011
    b>Entamoeba histolytica is a major health threat to people in developing countries, where it causes invasive diarrhea and liver abscesses...
  42. pmc Diagnosis of amebic liver abscess and amebic colitis by detection of Entamoeba histolytica DNA in blood, urine, and saliva by a real-time PCR assay
    Rashidul Haque
    International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1000, Bangladesh
    J Clin Microbiol 48:2798-801. 2010
    ..challenging, as most patients at the time of diagnosis do not have a concurrent intestinal infection with Entamoeba histolytica. Fecal testing for E. histolytica parasite antigen or DNA is negative in most patients...
  43. ncbi Impact of intestinal colonization and invasion on the Entamoeba histolytica transcriptome
    Carol A Gilchrist
    Department of Internal Medicine, University of Virginia, Charlottesville, VA 22908 1340, USA
    Mol Biochem Parasitol 147:163-76. 2006
    A genome-wide transcriptional analysis of Entamoeba histolytica was performed on trophozoites isolated from the colon of six infected mice and from in vitro culture...
  44. pmc Mitosomes in Entamoeba histolytica contain a sulfate activation pathway
    Fumika Mi-ichi
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Proc Natl Acad Sci U S A 106:21731-6. 2009
    ..To understand the central role of mitosomes in Entamoeba histolytica, a parasitic protozoon that causes amoebic dysentery and liver abscesses, we examined the proteomic ..
  45. pmc Transcriptional silencing of an amoebapore gene in Entamoeba histolytica: molecular analysis and effect on pathogenicity
    Rivka Bracha
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    Eukaryot Cell 2:295-305. 2003
    Transcriptional silencing of the gene coding for amoebapore A (AP-A) was observed when trophozoites of Entamoeba histolytica were transfected with a hybrid plasmid construct containing the ap-a gene flanked by the upstream and downstream ..
  46. ncbi Glycolysis in Entamoeba histolytica. Biochemical characterization of recombinant glycolytic enzymes and flux control analysis
    Emma Saavedra
    Departamento de Bioquimica, Instituto Nacional de Cardiologia, Tlalpan, Mexico DF, Mexico
    FEBS J 272:1767-83. 2005
    The synthesis of ATP in the human parasite Entamoeba histolytica is carried out solely by the glycolytic pathway. Little kinetic and structural information is available for most of the pathway enzymes...
  47. pmc Structural and biochemical studies of serine acetyltransferase reveal why the parasite Entamoeba histolytica cannot form a cysteine synthase complex
    Sudhir Kumar
    School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
    J Biol Chem 286:12533-41. 2011
    Cysteine (Cys) plays a major role in growth and survival of the human parasite Entamoeba histolytica. We report here the crystal structure of serine acetyltransferase (SAT) isoform 1, a cysteine biosynthetic pathway enzyme from E...
  48. ncbi Overexpression of myosin IB in living Entamoeba histolytica enhances cytoplasm viscosity and reduces phagocytosis
    Sabrina Marion
    Unité de Biologie Cellulaire du Parasitisme, INSERM U389, Institut Pasteur, 28 rue du Dr Roux 75724, Paris Cedex 15, France
    J Cell Sci 117:3271-9. 2004
    The human parasite Entamoeba histolytica is an ancient protozoan that expresses only one unconventional myosin, which has homology with myosin IB from other amoebae. Myosin IB is involved in phagocytosis of human cells by E. histolytica...
  49. ncbi Entamoeba histolytica: identification of EhGPCR-1, a novel putative G protein-coupled receptor that binds to EhRabB
    Karina Picazarri
    Departamento de Patologia Experimental, Centro de Investigacion y de Estudios Avanzados del IPN, A P 14 740, Mexico, D F 07000, Mexico
    Exp Parasitol 110:253-8. 2005
    EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E...
  50. pmc Entamoeba histolytica cysteine proteinase 5 binds integrin on colonic cells and stimulates NFkappaB-mediated pro-inflammatory responses
    YongZhong Hou
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 285:35497-504. 2010
    ..b>Entamoeba histolytica is a protozoan parasite that colonizes the gut, and in 10% of infected individuals, causes amebic colitis ..
  51. ncbi Persistence of secretory antiamoebic antibodies in patients with past invasive intestinal or hepatic amoebiasis
    O Valenzuela
    Departamento de Medicina Experimental, Facultad de Medicina, UNAM, ,
    Parasitol Res 87:849-52. 2001
    ..A total of 83% of these individuals developed high levels of serum anti-Entamoeba histolytica IgA. However, only 10.4% of the dysentery patients showed this anti-E...
  52. ncbi Induction of permeability changes and death of vertebrate cells is modulated by the virulence of Entamoeba spp. isolates
    James A Dvorak
    Laboratory of Malaria and Vector Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0425, USA
    Parasitol Int 52:169-73. 2003
    Although Entamoeba histolytica is capable of inducing an apoptotic response in vertebrate cells in vitro (Cell. Microbiol...
  53. ncbi Identification and evaluation of inhibitors of the EhGEF1 protein from Entamoeba histolytica
    N A Hernández-Cuevas
    Departamento de Biomedicina Molecular, Centro de Investigacion y de Estudios Avanzados del IPN, Mexico DF, Mexico
    J Mol Recognit 24:935-44. 2011
    ..It has been reported that members of the Dbl family have important roles in several cellular events in Entamoeba histolytica. These include activation of the actin cytoskeleton, cytokinesis, capping, uroid formation, cellular ..
  54. ncbi Host tissue invasion by Entamoeba histolytica is powered by motility and phagocytosis
    Elisabeth Labruyère
    Unité de Biologie Cellulaire du Parasitisme, INSERM U389, Institut Pasteur, Paris, France
    Arch Med Res 37:253-8. 2006
    ..histolytica require an active actin-myosin cytoskeleton to cap surface receptors, to adhere to host components, to migrate through tissues, to phagocyte human cells and to form liver abscesses...
  55. pmc Comparison of a stool antigen detection kit and PCR for diagnosis of Entamoeba histolytica and Entamoeba dispar infections in asymptomatic cyst passers in Iran
    Shahram Solaymani-Mohammadi
    Division of Intestinal and Genetal Protozoal Diseases, Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran Universoty of Medical Sciences, 14155 Tehran 6446, Iran
    J Clin Microbiol 44:2258-61. 2006
    ..histolytica II stool antigen kit and those from nested PCR. We concluded that E. dispar is much more common in asymptomatic cyst passers in Iran and that antigen detection and PCR are comparable diagnostic modalities...
  56. pmc Seroprevalence of Entamoeba histolytica infection in China
    Bin Yang
    Department of Microbiology and Parasitology, Shanghai Medical College, Fudan University, Shanghai, China
    Am J Trop Med Hyg 87:97-103. 2012
    The seroprevalence of Entamoeba histolytica infection in the residents of seven provinces in China was examined by using an enzyme-linked immunosorbent assay with a crude antigen and a recombinant surface antigen, C-Igl, of the parasites...
  57. pmc Overexpression of a mutant form of EhRabA, a unique Rab GTPase of Entamoeba histolytica, alters endoplasmic reticulum morphology and localization of the Gal/GalNAc adherence lectin
    B H Welter
    Department of Biological Sciences, Clemson University, Clemson, South Carolina 29634, USA
    Eukaryot Cell 8:1014-26. 2009
    b>Entamoeba histolytica is a protozoan parasite that causes amoebic dysentery and liver abscess. Vesicle trafficking events, such as phagocytosis and delivery of plasma membrane proteins, have been implicated in pathogenicity...
  58. ncbi Rab11B small GTPase regulates secretion of cysteine proteases in the enteric protozoan parasite Entamoeba histolytica
    Biswa Nath Mitra
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 851, Japan
    Cell Microbiol 9:2112-25. 2007
    Vesicular trafficking plays a pivotal role in the virulence of the enteric protozoan parasite Entamoeba histolytica. In the present study, we showed that one isotype of the small GTPase Rab11, EhRab11B, plays a central role in the ..
  59. ncbi Efficacy of a Gal-lectin subunit vaccine against experimental Entamoeba histolytica infection and colitis in baboons (Papio sp.)
    Mohamed D Abd Alla
    Department of Medicine, Medical College of Wisconsin, United States
    Vaccine 30:3068-75. 2012
    ..a Gal-lectin based intranasal synthetic peptide vaccine, we developed a new experimental primate model of Entamoeba histolytica intestinal infection. Release of xenic E...
  60. ncbi Acetaldehyde/alcohol dehydrogenase-2 (EhADH2) and clathrin are involved in internalization of human transferrin by Entamoeba histolytica
    Magda Reyes-López
    Programa de Doctorado en Ciencias Biológicas de la Universidad Autónoma Metropolitana, Apdo Postal 23 181, Mexico, DF 04960, Mexico
    Microbiology 157:209-19. 2011
    ..b>Entamoeba histolytica is a parasitic protozoan able to use holoTf as a sole iron source in vitro...
  61. ncbi Epithelial induction of porcine suppressor of cytokine signaling 2 (SOCS2) gene expression in response to Entamoeba histolytica
    Timothée Bruel
    Institut National de la Recherche Agronomique, UR1282, Infectiologie Animale et Sante Publique, Nouzilly, Tours, France
    Dev Comp Immunol 34:562-71. 2010
    ..following article, we used porcine polarized intestinal cells to study early response to the protozoan, Entamoeba histolytica, and we identified by rapid amplification of cDNA ends (RACE) PCR porcine SOCS1, SOCS4, SOCS5 and SOCS6 ..
  62. pmc Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient mice
    S L Stanley
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110
    Infect Immun 63:1587-90. 1995
    Evidence from in vitro studies suggest that the Entamoeba histolytica cysteine proteinase plays a role in the tissue lysis and cytopathic effects seen in invasive amebiasis. We used affinity-purified antibodies against a recombinant E...
  63. pmc Endoplasmic reticulum stress-sensing mechanism is activated in Entamoeba histolytica upon treatment with nitric oxide
    Julien Santi-Rocca
    Institut Pasteur, Unité Biologie Cellulaire du Parasitisme, Paris, France
    PLoS ONE 7:e31777. 2012
    ..The Entamoeba histolytica endomembrane system is simple compared to those of higher eukaryotes, as a canonical ER is not observed...
  64. pmc Analysis of Entamoeba histolytica excretory-secretory antigen and identification of a new potential diagnostic marker
    Weng Kin Wong
    Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia
    Clin Vaccine Immunol 18:1913-7. 2011
    ..Western blots using excretory-secretory antigen (ESA) collected from axenically grown Entamoeba histolytica were probed with the above serum samples...
  65. pmc Identification of four Entamoeba histolytica organellar DNA polymerases of the family B and cellular localization of the Ehodp1 gene and EhODP1 protein
    Maria Esther Herrera-Aguirre
    Posgrado en Ciencias Genomicas, Universidad Autonoma de la Ciudad de Mexico, San Lorenzo 290, Col del Valle, CP 03100 México, DF, Mexico
    J Biomed Biotechnol 2010:734898. 2010
    ..of a family of four active genes (Ehodp1, Ehodp2, Ehodp3, and Ehodp4) encoding putative DNA polymerases in Entamoeba histolytica, the protozoan parasite responsible of human amoebiasis...
  66. pmc Laboratory diagnosis of amebiasis
    Mehmet Tanyuksel
    Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Etlik, Ankara 06018, Turkey
    Clin Microbiol Rev 16:713-29. 2003
    The detection of Entamoeba histolytica, the causative agent of amebiasis, is an important goal of the clinical microbiology laboratory. To assess the scope of E. histolytica infection, it is necessary to utilize accurate diagnostic tools...
  67. ncbi Benzotriazoles and indazoles are scaffolds with biological activity against Entamoeba histolytica
    Fabian López-Vallejo
    Torrey Pines Institute for Molecular Studies, 11350 SW Village Parkway, Port St Lucie, FL 34987, USA
    J Biomol Screen 16:862-8. 2011
    Parasitic infections caused by Entamoeba histolytica are still major threats against public health, especially in developing countries...
  68. ncbi Bursera fagaroides, effect of an ethanolic extract on ornithine decarboxylase (ODC) activity in vitro and on the growth of Entamoeba histolytica
    Paulina Rosas-Arreguín
    Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal No 187, Guanajuato, Gto 36000, Mexico
    Exp Parasitol 119:398-402. 2008
    ..stem bark of Bursera fagaroides on ornithine decarboxylase (ODC) activity in vitro and on the growth of Entamoeba histolytica was evaluated. For this purpose, increasing concentrations of the extract, up to 8...
  69. ncbi Crystal structure of native O-acetyl-serine sulfhydrylase from Entamoeba histolytica and its complex with cysteine: structural evidence for cysteine binding and lack of interactions with serine acetyl transferase
    Krishna Chinthalapudi
    School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
    Proteins 72:1222-32. 2008
    ..The C-terminal helix positioning in the EhOASS may effect its interactions with EhSAT and thus influencing the formation of the cysteine synthase complex in this organism...
  70. ncbi Differences in morphology of phagosomes and kinetics of acidification and degradation in phagosomes between the pathogenic Entamoeba histolytica and the non-pathogenic Entamoeba dispar
    Biswa Nath Mitra
    Department of Parasitology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371 851, Japan
    Cell Motil Cytoskeleton 62:84-99. 2005
    Phagocytosis plays an important role in the pathogenicity of the intestinal protozoan parasite Entamoeba histolytica. We compared the morphology of phagosomes and the kinetics of phagosome maturation using conventional light and electron ..
  71. pmc Loss of dsRNA-based gene silencing in Entamoeba histolytica: implications for approaches to genetic analysis
    Ryan C MacFarlane
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305 5124, USA
    Exp Parasitol 119:296-300. 2008
    The ability to regulate gene expression in the protozoan parasite Entamoeba histolytica is critical in determining gene function. We previously published that expression of dsRNA specific to E...
  72. ncbi Entamoeba histolytica infection in men who have sex with men
    Chien Ching Hung
    Department of Internal Medicine, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan
    Lancet Infect Dis 12:729-36. 2012
    b>Entamoeba histolytica infection (amoebiasis) is the second leading cause of death from parasitic diseases...
  73. ncbi Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde-alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica
    Erika Pineda
    Departamento de Bioquimica, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico DF, Mexico
    FEBS J 277:3382-95. 2010
    The in vitro Entamoeba histolytica pyruvate:ferredoxin oxidoreductase (EhPFOR) kinetic properties and the effect of oxidative stress on glycolytic pathway enzymes and fluxes in live trophozoites were evaluated...
  74. ncbi Cysteine proteinases from distinct cellular compartments are recruited to phagocytic vesicles by Entamoeba histolytica
    Xuchu Que
    Department of Pathology, University of California, San Diego, UCSD Medical Center, 200 West Arbor Drive, San Diego, CA 92103 8416, USA
    Mol Biochem Parasitol 119:23-32. 2002
    ..encoded by at least seven genes, play a critical role in the pathogenesis of invasive amebiasis caused by Entamoeba histolytica. The study of these enzymes has been hampered by the inability to obtain significant quantities of the ..
  75. ncbi Newly visualized fibrillar collagen scaffolds dictate Entamoeba histolytica invasion route in the human colon
    Roman Thibeaux
    Institut Pasteur, Unité Biologie Cellulaire du Parasitisme, Paris, France INSERM U786, Paris, France
    Cell Microbiol 14:609-21. 2012
    ..determined the impact of the collagen fibres architecture on the invasive process of the enteric parasite Entamoeba histolytica. The behaviour of E...
  76. ncbi The genome of the protist parasite Entamoeba histolytica
    Brendan Loftus
    TIGR, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 433:865-8. 2005
    b>Entamoeba histolytica is an intestinal parasite and the causative agent of amoebiasis, which is a significant source of morbidity and mortality in developing countries. Here we present the genome of E...
  77. ncbi Entamoeba histolytica Schaudinn, 1903 and Entamoeba dispar Brumpt, 1925: differences in their cell surfaces and in the bacteria-containing vacuoles
    Paulo F P Pimenta
    Laboratory of Medical Entomology, Centro de Pesquisas Rene Rachou, Fundacao Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil
    J Eukaryot Microbiol 49:209-19. 2002
    b>Entamoeba histolytica Schaudinn, 1903 and Entamoeba dispar Brumpt. 1925 are two of eight species of Entamoeba that sometimes inhabit the human colon...
  78. pmc Evidence of a continuous endoplasmic reticulum in the protozoan parasite Entamoeba histolytica
    Jose E Teixeira
    Department of Medicine, University of Vermont College of Medicine, Burlington, VT 05405, USA
    Eukaryot Cell 7:1222-6. 2008
    b>Entamoeba histolytica, the cause of amebiasis, is believed to have no continuous endoplasmic reticulum (ER), with ER functions occurring in vesicles...
  79. ncbi NOX1 participates in ROS-dependent cell death of colon epithelial Caco2 cells induced by Entamoeba histolytica
    Kyeong Ah Kim
    Department of Environmental Medical Biology, Yonsei University College of Medicine, 134 Sinchon dong, Seodaemun gu, Seoul 120 752, Republic of Korea
    Microbes Infect 13:1052-61. 2011
    b>Entamoeba histolytica, which causes amebic colitis and occasional liver abscesses in humans, can induce host cell death through apoptosis and necrosis. Recently, we have demonstrated that E...
  80. ncbi Structure and content of the Entamoeba histolytica genome
    C G Clark
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Adv Parasitol 65:51-190. 2007
    The intestinal parasite Entamoeba histolytica is one of the first protists for which a draft genome sequence has been published...
  81. ncbi Overdiagnosis of amoebiasis in the absence of Entamoeba histolytica among patients presenting with diarrhoea in Wonji and Akaki, Ethiopia
    Amha Kebede
    Ethiopian Health and Nutrition Research and Ethio Netherlands AIDS Research Project, Addis Ababa, Ethiopia
    Trans R Soc Trop Med Hyg 97:305-7. 2003
    ..taking place, stool samples of 232 patients with complaints of diarrhoea were examined for the presence of Entamoeba histolytica and E. dispar DNA between April and December 2001...
  82. ncbi Methods for the investigation of diversity in Entamoeba histolytica
    C Graham Clark
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
    Arch Med Res 37:258-62. 2006
    The ability to distinguish variants of a species has many potential applications. In Entamoeba histolytica the first method to detect variation was based on isoenzyme analysis...
  83. ncbi Entamoeba histolytica uses ferritin as an iron source and internalises this protein by means of clathrin-coated vesicles
    Fernando López-Soto
    Departamento de Biologia Celular, Centro de Investigacion y de Estudios Avanzados del IPN, Mexico DF 07000, Mexico
    Int J Parasitol 39:417-26. 2009
    b>Entamoeba histolytica is a parasitic protozoan that produces dysentery and often reaches the liver, leading to abscess formation. Ferritin is an iron-storage protein that is mainly found in liver and spleen in mammals...
  84. ncbi Entamoeba histolytica: an update
    William Stauffer
    Department of Internal Medicine, Division of Infectious Disease and International Medicine, University of Minnesota, Minneapolis 55455, USA
    Curr Opin Infect Dis 16:479-85. 2003
    Over the past decade, since it was formally recognized that Entamoeba histolytica and Entamoeba dispar were two distinct species, studies in this field have made dramatic in-roads into the understanding of E...
  85. pmc A novel Entamoeba histolytica cysteine proteinase, EhCP4, is key for invasive amebiasis and a therapeutic target
    Chen He
    Department of Pathology and Medicine, University of California, San Diego, California 92103 8416, USA
    J Biol Chem 285:18516-27. 2010
    b>Entamoeba histolytica cysteine proteinases (EhCPs) play a key role in disrupting the colonic epithelial barrier and the innate host immune response during invasion of E. histolytica, the protozoan cause of human amebiasis...
  86. ncbi A possible cluster of sexually transmitted Entamoeba histolytica: genetic analysis of a highly virulent strain
    Irving E Salit
    Division of Infectious Diseases, Department of Medicine, University Health Network, Toronto General Hospital, Toronto, Ontario, Canada
    Clin Infect Dis 49:346-53. 2009
    Transmission of Entamoeba histolytica generally occurs by fecal excretion of cysts followed by oral ingestion of contaminated food or water...
  87. ncbi Entamoeba histolytica: apoptosis induced in vitro by nitric oxide species
    Espiridión Ramos
    Departamento de Medicina, Experimental, Facultad de Medicina Universidad Nacional Autonoma de Mexico, Mexico DF, Mexico
    Exp Parasitol 116:257-65. 2007
    ..This phenomenon has not been observed yet in Entamoeba histolytica. This work analyzed the in vitro effect of sodium nitroprusside, sodium nitrite and sodium nitrate (NOs) ..
  88. pmc Using differential gene expression to study Entamoeba histolytica pathogenesis
    Carol A Gilchrist
    Division of Infectious Diseases and International Health, Departments of Medicine, Microbiology and Pathology, University of Virginia, PO Box 801340, Charlottesville, VA 22908 1340, USA
    Trends Parasitol 25:124-31. 2009
    The release of the Entamoeba histolytica genome has facilitated the development of techniques to survey rapidly and to relate gene expression with biology...
  89. pmc Differences in the transcriptome signatures of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties
    Laura Biller
    Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str, 74, 20359 Hamburg, Germany
    BMC Genomics 11:63. 2010
    The availability of two genetically very similar cell lines (A and B) derived from the laboratory isolate Entamoeba histolytica HM-1:IMSS, which differ in their virulence properties, provides a powerful tool for identifying pathogenicity ..
  90. ncbi Structure and function of the Entamoeba histolytica Gal/GalNAc lectin
    Barbara J Mann
    Department of Internal Medicine and Microbiology, University of Virginia, Charlottesville 22908, USA
    Int Rev Cytol 216:59-80. 2002
    Gal/GalNAc lectin is a novel multifunctional virulence factor of the human parasite Entamoeba histolytica. The native protein is a 260-kDa heterodimer consisting of a type 1 membrane protein disulfide bonded to a lipid-anchored protein...
  91. pmc Epithelial cell apoptosis facilitates Entamoeba histolytica infection in the gut
    Stephen M Becker
    Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, VA 22908, USA
    Am J Pathol 176:1316-22. 2010
    b>Entamoeba histolytica is the protozoan parasite that causes amebic colitis. The parasite triggers apoptosis on contact with host cells; however, the biological significance of this event during intestinal infection is unclear...
  92. ncbi A novel ADP/ATP transporter in the mitosome of the microaerophilic human parasite Entamoeba histolytica
    Ka Wai Chan
    Dunn Human Nutrition Unit, Medical Research Council, Cambridge, UK
    Curr Biol 15:737-42. 2005
    ..We determined the mitochondrial carrier family (MCF) complement of the Entamoeba histolytica mitochondrial homolog, also known as a crypton [5] or more commonly as a mitosome [3]...
  93. pmc Use of bacterially expressed dsRNA to downregulate Entamoeba histolytica gene expression
    Carlos F Solis
    Institut Pasteur, Unité Biologie Cellulaire du Parasitisme, Paris, France
    PLoS ONE 4:e8424. 2009
    ..synthesized hairpin RNA are valuable tools for the analysis of gene function in the protozoan parasite Entamoeba histolytica. However, these approaches still require time-consuming procedures including transfection and drug ..
  94. pmc The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic
    Sudip K Ghosh
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts, USA
    PLoS Negl Trop Dis 4:e750. 2010
    The infectious and diagnostic form of Entamoeba histolytica (Eh), cause of amebic dysentery and liver abscess, is the quadranucleate cyst...
  95. ncbi The endoplasmic reticulum chaperone calreticulin is recruited to the uropod during capping of surface receptors in Entamoeba histolytica
    Fabienne Girard-Misguich
    Institut Pasteur, Unité Biologie Cellulaire du Parasitisme, Paris, France
    Mol Biochem Parasitol 157:236-40. 2008
    ..Here, we report descriptive data on the localization of Entamoeba histolytica's CRT at rest and following cap formation by Concanavalin A. As expected, CRT from E...
  96. ncbi Molecular cloning and characterization of the genes encoding two isoforms of cysteine synthase in the enteric protozoan parasite Entamoeba histolytica
    T Nozaki
    Department of Tropical Medicine and Parasitology, Keio University, School of Medicine, Tokyo, Japan
    Mol Biochem Parasitol 97:33-44. 1998
    The enteric protozoan parasite Entamoeba histolytica was shown to possess cysteine synthase (CS) activity. The cDNA and genomic clones that encode two isoforms of the E...
  97. pmc Metabolome analysis revealed increase in S-methylcysteine and phosphatidylisopropanolamine synthesis upon L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica
    Afzal Husain
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 285:39160-70. 2010
    ..In the protozoan parasite Entamoeba histolytica, the causative agent of amebiasis, L-cysteine plays an essential role in proliferation, adherence, and ..
  98. pmc Bioinformatic analysis of Entamoeba histolytica SINE1 elements
    Derek M Huntley
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
    BMC Genomics 11:321. 2010
    Invasive amoebiasis, caused by infection with the human parasite Entamoeba histolytica remains a major cause of morbidity and mortality in some less-developed countries. Genetically E...
  99. ncbi Calpain-dependent cleavage of SHP-1 and SHP-2 is involved in the dephosphorylation of Jurkat T cells induced by Entamoeba histolytica
    K A Kim
    Department of Environmental Medical Biology, Institute of Tropical Medicine, and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
    Parasite Immunol 32:176-83. 2010
    Host cell death induced by Entamoeba histolytica is an important mechanism for both host defence and microbial immune evasion during human amoebiasis. However, the signalling pathways underlying cell death induced by E...
  100. pmc Two atypical L-cysteine-regulated NADPH-dependent oxidoreductases involved in redox maintenance, L-cystine and iron reduction, and metronidazole activation in the enteric protozoan Entamoeba histolytica
    Ghulam Jeelani
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 285:26889-99. 2010
    ..activities of two atypical NADPH-dependent oxidoreductases (EhNO1/2) from the enteric protozoan parasite Entamoeba histolytica. EhNO1/2 were previously annotated as the small subunit of glutamate synthase (glutamine:2-oxoglutarate ..
  101. pmc Entamoeba histolytica-induced dephosphorylation in host cells
    Jose E Teixeira
    Department of Internal Medicine, University of Virginia, School of Medicine, Charlottesville, Virginia 22908, USA
    Infect Immun 70:1816-23. 2002
    ..To determine whether protein tyrosine dephosphorylation played a role in signaling pathways affecting Entamoeba histolytica-mediated host cell killing, we investigated the involvement of PTPases during the attachment of E...

Research Grants64

  1. ENTAMOEBA HISTOLYTICA GENOME PROJECT
    Brendan Loftus; Fiscal Year: 2002
    DESCRIPTION (Adapted from the Investigator's Abstract): Entamoeba histolytica is a protozoan parasite that causes tens of millions of cases of dysentery and liver abscess in Latin America, Africa and Asia...
  2. New Rapid Diagnostic Tests for Amebiasis
    JOEL F HERBEIN; Fiscal Year: 2010
    Our goal is to produce point-of-care diagnostic tests for amebiasis. Entamoeba histolytica is a protozoan parasite and the causative agent of amebiasis in humans...
  3. Multiplexed Detection of Food and Waterborne Pathogens
    Francis Barany; Fiscal Year: 2011
    ..to detect the Category B food and water-borne protozoa (Cryptosporidium parvum, Cyclospora cayatanensis, Entamoeba histolytica, Giardia lamblia and Microsporidia) and viruses (Caliciviruses, Hepatitis A virus and Rotavirus) ..
  4. Molecular Mechanism of Entamoeba histolytica phagocytosis
    Christopher D Huston; Fiscal Year: 2012
    Amebiasis ranks second as a protozoan cause of death. Entamoeba histolytica, the etiologic agent, is an intestinal ameba that acquires nutrients by phagocytosis of colonic bacteria, and phagocytosis of host cells by E...
  5. Maternal Intestinal Infections and Low Birth Weight in a Rural Indian Cohort
    RACHEL MARGARET WHELAN; Fiscal Year: 2012
    ..This proposal is for an investigation of the role of chronic parasitic infections (particularly giardia, entamoeba histolytica and helminthic infections) as contributors to low birth weight, preterm delivery and other birth outcomes ..
  6. Alum-absorbed subunit vaccine to prevent intestinal amebiasis
    David M Lyerly; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): We propose to produce a subunit vaccine for Entamoeba histolytica and test it in mice for immunogenicity and for protection from intestinal amebiasis...
  7. A forward genetics screen for genes regulating phagocytosis and signaling in Enta
    Lesly A Temesvari; Fiscal Year: 2010
    b>Entamoeba histolytica is the causative agent of amoebic dysentery and liver abscess...
  8. Novel Therapeutics for Class B Protozoa
    Sharon L Reed; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Entamoeba histolytica and Giardia lamblia are Class B protozoa, which are major causes of water- and foodborne epidemics worldwide...
  9. Role of lipid rafts and phosphoinositides in E. histolytica virulence
    Lesly A Temesvari; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Entamoeba histolytica is the causative agent of amoebic dysentery and liver abscess. It ranks third as a worldwide cause of morbidity and has been classified as a Category B bioterrorism agent...
  10. 2012 International Conference on Anaerobic Parasites (ICAP)
    PATRICIA JEAN JOHNSON; Fiscal Year: 2012
    ..This meeting will focus on 3 related anaerobic human parasites, Entamoeba histolytica, Giardia intestinalis, and Trichomonas vaginalis with worldwide distribution, presenting public health ..
  11. Development of Genetic Tools in Entamoeba histolytica
    Upinder Singh; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The protozoan parasite Entamoeba histolytica causes dysentery and liver abscesses and is one of the most common causes of death from parasitic disease worldwide...
  12. The Role of Leptin in Susceptibility to Amebiasis
    Chelsea S Marie; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Entamoeba histolytica is the pathogenic protozoan responsible for intestinal amebiasis and amebic liver abscess. E...
  13. Elucidating a G-protein signaling pathway in E. histolytica amoebic colitis
    Dustin E Bosch; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Ameobic colitis, caused by Entamoeba histolytica, is a major world health burden, resulting in an estimated 100,000 deaths per year...
  14. Point-of-Care Diagnostic Test for Entamoeba histolytica Cysts
    JOEL F HERBEIN; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The NIAID Category B priority pathogen Entamoeba histolytica is a public health threat, especially as a cause of diarrhea in travelers to and immigrants from endemic areas, men who have sex with men, ..
  15. Investigating Entamoeba histolytica Colonic Pathogenesis
    Upinder Singh; Fiscal Year: 2012
    The protozoan parasite Entamoeba histolytica causes an estimated 50 million cases of invasive disease annually. The most common manifestations of amebic infection are colonic disease and liver abscesses...
  16. Development of an E. histolytica Multilocus Genotyping System
    Carol A Gilchrist; Fiscal Year: 2013
    ..The eukaryotic parasite Entamoeba histolytica is one of the top 10 causes of diarrhea in the developing world. The outcome of an E...
  17. NATURAL IMMUNITY TO ENTAMOEBA HISTOLYTICA
    JONATHAN RAVDIN; Fiscal Year: 1999
    ..This proposal will address an incredibly important issues in amebiasis research and have a major impact on vaccine development and clinical practice in the field. ..
  18. Transcriptome analysis of Entamoeba development using RNA-sequencing
    Upinder Singh; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Entamoeba histolytica infects over 500 million people annually and is a leading cause of parasitic death in humans. The infectious cycle of E...
  19. Multiple Lectin Model of the Cyst Wall of Entamoeba
    John C Samuelson; Fiscal Year: 2010
    ..b>Entamoeba histolytica (Eh) is a protozoan parasite that causes amebic dysentery and liver abscess...
  20. Multiplex Screening ELISA for Giardia, Cryptosporidium and E. histolytica
    JOEL F HERBEIN; Fiscal Year: 2010
    ..and Entamoeba histolytica in stool...
  21. Role of Leptin in Innate Mucosal Defense from Amebiasis
    William A Petri; Fiscal Year: 2013
    ..leptin receptor signaling in intestinal epithelial cells (IECs) protects from intestinal infection due to Entamoeba histolytica, both intrinsically within IECs by up-regulating repair pathways, and extrinsically by IEC-mediated ..
  22. Cooperative Research Partnership for an Amebic Colitis Vaccine
    William A Petri; Fiscal Year: 2010
    ..develop and test in mice an acellular vaccine for the prevention of amebic colitis due to infection with Entamoeba histolytica, an NIAID Category B priority pathogen...
  23. INTRASPECIES VARIATION IN ENTAMOEBA HISTOLYTICA
    William Petri; Fiscal Year: 1999
    DESCRIPTION Hypothesis: Genetically distinct isolates of Entamoeba histolytica exist within a defined geographic area and vary in invasiveness and organ tropism...
  24. Mouse Strain dependent innate resistance to intestinal amebiasis
    Eric Houpt; Fiscal Year: 2009
    b>Entamoeba histolytica kills more humans than any other Category B protozoa by far. This R01 proposes work by a new investigator to identify mechanisms of innate resistance to intestinal amoebiasis in a new mouse model...
  25. DESIGN AND SYNTHESIS OF CYSTEINE PROTEINASE INHIBITORS
    DANIEL RICH; Fiscal Year: 1992
    ..of the following cysteine proteinases: papain; cathepsin B, H, and L; the cysteine proteinase for entamoeba histolytica; and calpains I and II...
  26. New Diagnostics for Amebiasis
    David Lyerly; Fiscal Year: 2005
    b>Entamoeba histolytica, the cause of amoebiasis, is a Category B pathogen considered in the top three biodefense parasites because it is difficult to diagnose...
  27. MECHANISMS OF E HISTOLYTICA ADHERENCE AND CYTOLYSIS
    JONATHAN RAVDIN; Fiscal Year: 1993
    The enteric protozoan Entamoeba histolytica causes disease worldwide; parasite adherence and cytolytic events are crucial in pathogenesis of invasive amebiasis...
  28. TRANSCRIPTIONAL CONTROL IN ENTAMOEBA HISTOLYTICA
    Upinder Singh; Fiscal Year: 2001
    ..the role of three conserved and novel cis-acting regulatory sequences within the core promoter region of Entamoeba histolytica protein encoding genes...
  29. Microalgal Oral Vaccines for Control of Amebiasis
    Richard Wagner; Fiscal Year: 2004
    ..oral vaccine for the prevention of amebic dysentery in humans caused by the protozoan parasite Entamoeba histolytica. It is our hypothesis that an oral, microalgal-based vaccine delivery system is a cost-effective means to ..
  30. MOLECULAR GENETICS OF DRUG RESISTANCE IN E HISTOLYICA
    John Samuelson; Fiscal Year: 1993
    The goal of the proposed work is to investigate mechanism of drug resistance in Entamoeba histolytica, the protozoan parasite that causes human amoebiasis. Drug treatment is presently the major means of controlling E...
  31. SERODIAGNOSIS AND IMMUNOPROPHYLAXIS OF AMEBIASIS
    David Lyerly; Fiscal Year: 1999
    DESCRIPTION (Adapted from the Applicant's Abstract): The trophozoites of Entamoeba histolytica, the cause of human amebiasis adhere to colonic mucins, intestinal epithelium and leukocytes via a galactose-specific lectin...
  32. Shotgun Sequencing of the Trichomonas Vaginalis Genome
    Jane Carlton; Fiscal Year: 2003
    ..Use will be made of the sequence data from the Entamoeba histolytica and Giardia lamblia genome projects, in a comparative approach to gene identification...
  33. BIOCHEMICAL CYTOLOGY OF ANAEROBIC PARASITIC PROTISTS
    MIKLOS MULLER; Fiscal Year: 2003
    ..metabolism of the medically important cavity parasites of humans, the amitochondriate Giardia lamblia, Entamoeba histolytica and Trichomonas vaginalis differs markedly from that of their human host...
  34. FeS cluster assembly-- amitochondrial parasitic protists
    MIKLOS MULLER; Fiscal Year: 2003
    ..FeS) cluster assembly in amitochondriate parasites of importance in human (Trichonionas vaginalis, Entamoeba histolytica, Girdia lamblia) and veterinary (Tritrichomonas foetus) medicine...
  35. Identification of effectors of host cell killing by Entamoeba histolytica
    Katherine S Ralston; Fiscal Year: 2010
    ..Since infection is common in children in the developing world and is a significant cause of morbidity and mortality, these studies have promise to positively impact global child health. ..
  36. Regulation of rRNA transcription in Entamoeba histolyti*
    William Petri; Fiscal Year: 2003
    ..The aim of this proposal is to locate the regulatory elements required for transcription of rRNA genes in Entamoeba histolytica. Transcription of rDNA in E...
  37. NEW APPROACHES TO THE DIAGNOSIS OF AMEBIASIS
    David Lyerly; Fiscal Year: 2001
    Amebiasis, caused by Entamoeba histolytica, is a major problem in developing countries...
  38. BIOCHEMICAL CYTOLOGY OF GIARDIA AND ENTAMOEBA
    DONALD LINDMARK; Fiscal Year: 1980
    ..is to gain an understanding of the metabolism of two anaerobic protozoan parasites, Giardia lamblia and Entamoeba histolytica. Special emphasis will be placed on comparative aspects of anaerobic metabolism as to their ..
  39. INTERACTIONS OF C3 WITH BACTERIAL PATHOGENS
    MARGARET HOSTETTER; Fiscal Year: 2000
    ..cleavage due to serine proteinases, complement convertases, or organisms such as Pseudomonas aeruginosa or Entamoeba histolytica. Our recent experiments have shown that C3 degrading activity correlates directly with pneumococcal ..
  40. MOLECULAR PATHOGENESIS OF DIGESTIVE DISEASES
    HARRY BERNARD GREENBERG; Fiscal Year: 2010
    ..pylori, and diarrheal agents such as rotavirus, salmonella, E. coli, cholera, Entamoeba histolytica and the Whipple's bacillus...
  41. CYSTEINE PROTEINASES AND VIRULENCE OF E. HISTOLYTICA
    Sharon Reed; Fiscal Year: 2000
    ..Cysteine proteinases, which play a role in tissue invasion of host defenses, are a key virulence factor of Entamoeba histolytica. They have shown that purified proteinase of E...
  42. MUCOSAL IMMUNITY AND INFECTION
    MICHAEL LAMM; Fiscal Year: 2004
    ..Project 1 addresses Entamoeba histolytica and amoebiasis, a leading cause of parasitic death and morbidity worldwide...
  43. PATHWAYS REGULATING AMOEBIC CYST FORMATION
    Daniel Eichinger; Fiscal Year: 2009
    DESCRIPTION (provided by the applicant): Entamoeba histolytica infects approximately 1% of the world's population, and causes up to 100,000 deaths annually...
  44. Rapid Diagnostics for Category B Enteropathogens
    William Petri; Fiscal Year: 2007
    ..Successful completion of these studies will yield high throughput diagnostic tests that target multiple enteropathogens simultaneously in a single sample. ..
  45. ADHERENCE BLOCKING VACCINE FOR AMEBIASIS
    William Petri; Fiscal Year: 2003
    DESCRIPTION (adapted from the application): Entamoeba histolytica, the cause of amebic liver abscess and amebic dysentery represents a significant threat to public health worldwide...
  46. GENE EXPRESSION IN ENTAMOEBA HISTOLYTICA
    William Petri; Fiscal Year: 2008
    DESCRIPTION (provided by the applicant): We propose to study gene expression in Entamoeba histolytica controlled by the upstream regulatory element 3 binding protein (URE3-BP)...
  47. Developing Tools to Study Encystation in Entamoeba Histolytica
    Upinder Singh; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): The protozoan parasite Entamoeba histolytica causes an estimated 50 million cases of invasive disease annually and is the second leading parasitic cause of death worldwide...
  48. Adhesins and Invasins of Francisella Tularensis
    Barbara Mann; Fiscal Year: 2004
    ..Understanding the basic mechanisms of pathogenicity will lead to a greater understanding of why this organism is such an effective pathogen, and the identification of new targets for therapies. ..
  49. Assessing Genetic Diveristy in Entamoeba Histolytica
    Upinder Singh; Fiscal Year: 2006
    DESCRIPTION (provided by the applicant): The protozoan parasite Entamoeba histolytica causes an estimated 50 million cases of invasive disease annually and is the second leading parasitic cause of death worldwide...
  50. GALACTOSE LECTIN OF E HISTOLYTICA
    Barbara Mann; Fiscal Year: 2002
    b>Entamoeba histolytica is a human cytolytic parasite with worldwide distribution that infects up to 50 million people and results in an estimated 70,000 deaths annually. The E...
  51. Identification of Transcriptional Regulatory Networks in Entamoeba Histolytica
    Upinder Singh; Fiscal Year: 2008
    ..b>Entamoeba histolytica is a leading cause of parasitic death, but little is known about the virulence program that this protozoan ..
  52. EXPANDED PROTEIN STRUCTURE REFINEMENT AND VALIDATION
    Ethan Merritt; Fiscal Year: 2005
    ..This, in turn, will make it easier to identify key biological features such as the presence and conformation of bound ligands, and the nature of hinge and inter-domain motions. ..
  53. Software tools for analysis and visulaization of protein structure
    Ethan A Merritt; Fiscal Year: 2010
    ..The algorithms and computation tools developed as part of this work will be distributed freely, and we will provide web-based state of the art analysis of structures submitted by external researchers. ..
  54. Mucosal immune regulation in murine amebic colitis
    Eric Houpt; Fiscal Year: 2007
    ..The comprehensive approach in training and research laid out in this research proposal will guide the applicant towards his goal of being an independent investigator in immunoparasitology. ..
  55. MOLECULAR DISSECTION OF E. HISTOLYTICA PATHOGENESIS
    William F Stenson; Fiscal Year: 2010
    The protozoan parasite Entamoeba histolytica causes amebiasis, which remains a major health problem in much of the world, and is considered a potential biological weapon...
  56. RNA METABOLISM IN TRYPANOSOMES
    Elisabetta Ullu; Fiscal Year: 2009
    ..3. Further characterize the RNAi pathway in terms of its components and how the interaction of TbAGO1 with translating ribosomes is brought about. ..
  57. Identification of E. histolytica virulence factors
    Molly Hughes; Fiscal Year: 2004
    ..b>Entamoeba histolytica is one of the etiologic agents...
  58. Indoleamine 23-Dioxygnease in Intestinal Inflammation
    William F Stenson; Fiscal Year: 2010
    ..The long term goal of this project is to determine if there is a role for pharmacologic agents that increase IDO expression in the management of human inflammatory bowel disease. ..