acanthamoeba

Summary

Summary: A genus of free-living soil amoebae that produces no flagellate stage. Its organisms are pathogens for several infections in humans and have been found in the eye, bone, brain, and respiratory tract.

Top Publications

  1. ncbi Reduced expression of the global regulator protein CsrA in Legionella pneumophila affects virulence-associated regulators and growth in Acanthamoeba castellanii
    Vera Forsbach-Birk
    Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Str 8, D 8908I Uhn, Germany
    Int J Med Microbiol 294:15-25. 2004
  2. ncbi Isolation and identification of amoeba-resisting bacteria from water in human environment by using an Acanthamoeba polyphaga co-culture procedure
    Isabelle Pagnier
    Unite des Rickettsies, CNRS UMR 6020, Faculte de Medecine de Marseille, 13385 Marseille Cedex 05, France
    Environ Microbiol 10:1135-44. 2008
  3. ncbi Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea
    Govinda S Visvesvara
    Division of Parasitic Diseases, National Center for Infectious Diseases, Atlanta, Georgia 30341 3724, USA
    FEMS Immunol Med Microbiol 50:1-26. 2007
  4. pmc Giant virus with a remarkable complement of genes infects marine zooplankton
    Matthias G Fischer
    Department of Microbiology, University of British Columbia, Vancouver, BC, Canada V6T 1Z4
    Proc Natl Acad Sci U S A 107:19508-13. 2010
  5. pmc mRNA deep sequencing reveals 75 new genes and a complex transcriptional landscape in Mimivirus
    Matthieu Legendre
    Structural and Genomic Information Laboratory, Centre National de la Recherche Scientifique, UPR2589, Mediterranean Institute of Microbiology IFR88, Aix Marseille University, Parc Scientifique de Luminy, FR 13288 Marseille, France
    Genome Res 20:664-74. 2010
  6. ncbi Acanthamoeba affects the integrity of human brain microvascular endothelial cells and degrades the tight junction proteins
    Naveed Ahmed Khan
    School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, UK
    Int J Parasitol 39:1611-6. 2009
  7. pmc Viruses with more than 1,000 genes: Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, and reannotation of Mimivirus genes
    Philippe Colson
    Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes URMITE, Centre National de la Recherche Scientifique UMR IRD 6236, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    Genome Biol Evol 3:737-42. 2011
  8. ncbi Mimivirus
    J M Claverie
    Structural and Genomic Information Laboratory, Parc Scientifique de Luminy, Case 934 13288 Marseille cedex 09, France
    Curr Top Microbiol Immunol 328:89-121. 2009
  9. ncbi Tentative characterization of new environmental giant viruses by MALDI-TOF mass spectrometry
    Bernard La Scola
    Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille and URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    Intervirology 53:344-53. 2010
  10. ncbi Acanthamoeba keratitis: diagnosis and treatment update 2009
    John K G Dart
    Corneal and External Disease Service, Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom
    Am J Ophthalmol 148:487-499.e2. 2009

Research Grants

  1. CRISPR Cas genes and Legionella pneumophila infection
    Nicholas P Cianciotto; Fiscal Year: 2013
  2. New Therapies for Pathogenic Free-Living Amoebae
    Dennis E Kyle; Fiscal Year: 2013
  3. PATHOGENESIS OF ACANTHAMOEBA KERATITIS
    Noorjahan Panjwani; Fiscal Year: 2013
  4. Planning Grant for Parasitic Ulcer Treatment Trial
    Jeremy David Keenan; Fiscal Year: 2013
  5. Biology, Immunology &therapy of Acanthamoeba Keratitis
    Hassan Alizadeh; Fiscal Year: 2013
  6. GENETIC DETERMINANTS OF PATHOGENICITY IN LEGIONELLA
    Lucy Tompkins; Fiscal Year: 2001
  7. NEW TREATMENT FOR AMOEBIC KERATITIS
    CLARENCE VILLEMEZ; Fiscal Year: 2001
  8. THREE DIMENSIONAL STRUCTURE OF ALPHA ACTININ
    Kenneth Taylor; Fiscal Year: 2000
  9. RIBOSOMAL RNA TRANSCRIPTION FACTOR FUNCTIONAL ANALYSIS
    Marvin Paule; Fiscal Year: 1992
  10. PATHOGENESIS AND THERAPY OF ACANTHAMOEBA KERATITIS
    Michael Osato; Fiscal Year: 1990

Detail Information

Publications235 found, 100 shown here

  1. ncbi Reduced expression of the global regulator protein CsrA in Legionella pneumophila affects virulence-associated regulators and growth in Acanthamoeba castellanii
    Vera Forsbach-Birk
    Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Str 8, D 8908I Uhn, Germany
    Int J Med Microbiol 294:15-25. 2004
    ..Replication ability of the CsrA(-) mutant in amoebae was also affected. Based on these results, we could show that CsrA is involved in the regulation of the bacterial switch from the replicative to the transmissible form...
  2. ncbi Isolation and identification of amoeba-resisting bacteria from water in human environment by using an Acanthamoeba polyphaga co-culture procedure
    Isabelle Pagnier
    Unite des Rickettsies, CNRS UMR 6020, Faculte de Medecine de Marseille, 13385 Marseille Cedex 05, France
    Environ Microbiol 10:1135-44. 2008
    ..This preliminary work demonstrates that the water environment in the vicinity of humans is a reservoir of ARB, including well-known pathogens for which amoebae and/or water was not recognized earlier as a possible reservoir...
  3. ncbi Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea
    Govinda S Visvesvara
    Division of Parasitic Diseases, National Center for Infectious Diseases, Atlanta, Georgia 30341 3724, USA
    FEMS Immunol Med Microbiol 50:1-26. 2007
    ..free-living amoebae that exist in nature, members of only four genera have an association with human disease: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri and Sappinia diploidea. Acanthamoeba spp. and B...
  4. pmc Giant virus with a remarkable complement of genes infects marine zooplankton
    Matthias G Fischer
    Department of Microbiology, University of British Columbia, Vancouver, BC, Canada V6T 1Z4
    Proc Natl Acad Sci U S A 107:19508-13. 2010
    ..Phylogenetic analysis indicates that CroV is a nucleocytoplasmic large DNA virus, with Acanthamoeba polyphaga mimivirus as its closest relative, although less than one-third of the genes of CroV have homologs in ..
  5. pmc mRNA deep sequencing reveals 75 new genes and a complex transcriptional landscape in Mimivirus
    Matthieu Legendre
    Structural and Genomic Information Laboratory, Centre National de la Recherche Scientifique, UPR2589, Mediterranean Institute of Microbiology IFR88, Aix Marseille University, Parc Scientifique de Luminy, FR 13288 Marseille, France
    Genome Res 20:664-74. 2010
    Mimivirus, a virus infecting Acanthamoeba, is the prototype of the Mimiviridae, the latest addition to the nucleocytoplasmic large DNA viruses...
  6. ncbi Acanthamoeba affects the integrity of human brain microvascular endothelial cells and degrades the tight junction proteins
    Naveed Ahmed Khan
    School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, UK
    Int J Parasitol 39:1611-6. 2009
    Haematogenous spread is a key step in the development of Acanthamoeba granulomatous encephalitis, however it is not clear how circulating amoebae cross the blood-brain barrier to enter the CNS to produce disease...
  7. pmc Viruses with more than 1,000 genes: Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, and reannotation of Mimivirus genes
    Philippe Colson
    Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes URMITE, Centre National de la Recherche Scientifique UMR IRD 6236, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    Genome Biol Evol 3:737-42. 2011
    The genome sequence of the Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, is reported. With 1,191,693 nt in length and 1,023 predicted protein-coding genes, the Mamavirus has the largest genome among the known viruses...
  8. ncbi Mimivirus
    J M Claverie
    Structural and Genomic Information Laboratory, Parc Scientifique de Luminy, Case 934 13288 Marseille cedex 09, France
    Curr Top Microbiol Immunol 328:89-121. 2009
    b>Acanthamoeba polyphaga Mimivirus, the first representative and prototype member of the Mimiviridae, is the latest addition to the menagerie of lesser-known big DNA viruses...
  9. ncbi Tentative characterization of new environmental giant viruses by MALDI-TOF mass spectrometry
    Bernard La Scola
    Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille and URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    Intervirology 53:344-53. 2010
    Metagenomic studies have revealed that Acanthamoeba polyphaga Mimivirus relatives are common in the environment; however, only three Acanthamoeba-growing giant viruses have been isolated from hundreds of environmental samples...
  10. ncbi Acanthamoeba keratitis: diagnosis and treatment update 2009
    John K G Dart
    Corneal and External Disease Service, Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom
    Am J Ophthalmol 148:487-499.e2. 2009
    To describe the current management of Acanthamoeba keratitis (AK).
  11. pmc Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge
    J M Schroeder
    Department of Molecular Genetics, The Ohio State University, 484 W. 12th Ave, Columbus, OH 43210, USA
    J Clin Microbiol 39:1903-11. 2001
    This study identified subgenic PCR amplimers from 18S rDNA that were (i) highly specific for the genus Acanthamoeba, (ii) obtainable from all known genotypes, and (iii) useful for identification of individual genotypes...
  12. pmc Biology and pathogenesis of Acanthamoeba
    Ruqaiyyah Siddiqui
    The Aga Khan University, Karachi, Pakistan
    Parasit Vectors 5:6. 2012
    b>Acanthamoeba is a free-living protist pathogen, capable of causing a blinding keratitis and fatal granulomatous encephalitis...
  13. ncbi Parachlamydia acanthamoebae, an emerging agent of pneumonia
    G Greub
    Centre for Research on Intracellular Bacteria CRIB, Institute of Microbiology, University Hospital Centre and University of Lausanne, Lausanne, Switzerland
    Clin Microbiol Infect 15:18-28. 2009
    Parachlamydia acanthamoebae is a Chlamydia-like organism that easily grows within Acanthamoeba spp. Thus, it probably uses these widespread free-living amoebae as a replicative niche, a cosmopolite aquatic reservoir and a vector...
  14. ncbi Cloning and characterization of a novel mannose-binding protein of Acanthamoeba
    Marco Garate
    Department of Ophthalmology, Center for Vision Research and The New England Eye Center, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    J Biol Chem 279:29849-56. 2004
    Acanthamoebae produce a painful, blinding infection of the cornea. The mannose-binding protein (MBP) of Acanthamoeba is thought to play a key role in the pathogenesis of the infection by mediating the adhesion of parasites to the host ..
  15. ncbi In vivo and in vitro laser confocal microscopy to diagnose acanthamoeba keratitis
    Atsushi Shiraishi
    Department of Ophthalmology and Regenerative Medicine, Ehime University Graduate School of Medicine, Toon, Ehime, Japan
    Cornea 29:861-5. 2010
    To determine the effectiveness of laser confocal microscopy in identifying Acanthamoeba cysts and trophozoites in the cornea of patients with Acanthamoeba keratitis (AK) and to evaluate its effectiveness in following AK after treatment.
  16. ncbi Protease activities of Acanthamoeba polyphaga and Acanthamoeba castellanii
    José de Jesús Serrano-Luna
    Departamento de Biologia Celular, CINVESTAV IPN, Mexico, D F Mexico
    Can J Microbiol 52:16-23. 2006
    b>Acanthamoeba spp. are free-living amoebae that cause amoebic granulomatous encephalitis, skin lesions, and ocular amoebic keratitis in humans...
  17. pmc Mycobacterium tuberculosis complex mycobacteria as amoeba-resistant organisms
    Felix Mba Medie
    Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR 6236 CNRS Université de la Méditerranée, IRD 189, IFR 48, Faculte de Medecine, Marseille, France
    PLoS ONE 6:e20499. 2011
    ..An environmental source has been proposed for the animal Mycobacterium bovis and the human Mycobacterium canettii...
  18. pmc Amoebal coculture of "Mycobacterium massiliense" sp. nov. from the sputum of a patient with hemoptoic pneumonia
    Toidi Adekambi
    Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, Université de la Méditteranée, Marseille, France
    J Clin Microbiol 42:5493-501. 2004
    ..The type strain has been deposited in the Collection Institut Pasteur as CIP 108297(T) and in Culture Collection of the University of Goteborg, Goteborg, Sweden, as CCUG 48898(T)...
  19. ncbi Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida)
    Daniele Corsaro
    CHLAREAS, Chlamydia Research Association, 12 rue du Maconnais, 54500, Vandoeuvre les Nancy, France
    Parasitol Res 107:233-8. 2010
    b>Acanthamoeba are widespread free-living amoebae, able to cause infection in animals, with keratitis and granulomatous encephalitis as major diseases in humans...
  20. pmc Isolation of Legionella anisa using an amoebic coculture procedure
    B La Scola
    , CNRS UPRESA 6020, , 13385 Marseille Cedex 05, France
    J Clin Microbiol 39:365-6. 2001
    ..However, coculture of a sputum sample with Acanthamoeba polyphaga amoebae led to the recovery of Legionella anisa...
  21. pmc Waterborne protozoan pathogens
    M M Marshall
    Department of Veterinary Science, University of Arizona, Tucson 85721, USA
    Clin Microbiol Rev 10:67-85. 1997
    ..The waterborne parasites Giardia lamblia, Naegleria fowleri, Acanthamoeba spp...
  22. pmc Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections
    Gregory C Booton
    Department of Evolution, Ecology, Organismal Biology, The Ohio State University, 318 W 12th Ave, Columbus, OH 43210, USA
    J Clin Microbiol 43:1689-93. 2005
    b>Acanthamoeba is a free-living protozoan genus found in a wide variety of natural habitats, including water, soil, and air...
  23. ncbi Acanthamoeba polyphaga resuscitates viable non-culturable Legionella pneumophila after disinfection
    Maria Teresa Garcia
    Department of Microbiology and Immunology, University of Louisville, Louisville, KY, USA
    Environ Microbiol 9:1267-77. 2007
    ..Our work showed that in the absence of Acanthamoeba polyphaga, seven L. pneumophila strains became non-culturable after treatment by 256 p.p.m...
  24. pmc Laboratory diagnosis of amoebic keratitis: comparison of four diagnostic methods for different types of clinical specimens
    Andrea K Boggild
    Tropical Diseases Unit, Toronto General Hospital, Toronto, Ontario, Canada
    J Clin Microbiol 47:1314-8. 2009
    ..for acanthamoebae by direct smear analysis, culture, and PCR using two different primer sets specific for Acanthamoeba ribosomal DNA...
  25. pmc Purification and characterization of a 33 kDa serine protease from Acanthamoeba lugdunensis KA/E2 isolated from a Korean keratitis patient
    Hyo Kyung Kim
    Department of Neurology, Ulsan University College of Medicine, Ulsan 680 060, Republic of Korea
    Korean J Parasitol 41:189-96. 2003
    ..proteases in the pathogenesis of amoebic keratitis, we purified and characterized a serine protease secreted by Acanthamoeba lugdunensis KA/E2, isolated from a Korean keratitis patient...
  26. ncbi Isolation and genotyping of potentially pathogenic Acanthamoeba and Naegleria species from tap-water sources in Osaka, Japan
    Akiko Edagawa
    Division of Environmental Health, Osaka Prefectural Institute of Public Health, Osaka, Japan
    Parasitol Res 105:1109-17. 2009
    ..chain reaction and sequence analysis were then performed to define the species of the detected Naegleria and Acanthamoeba isolates. A total of 257 of 374 samples (68...
  27. pmc Identification of a novel t17 genotype of acanthamoeba from environmental isolates and t10 genotype causing keratitis in Thailand
    Warisa Nuprasert
    Molecular Biology of Malaria and Opportunistic Parasites Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
    J Clin Microbiol 48:4636-40. 2010
    We analyzed the nuclear small-subunit rRNA genes of Acanthamoeba isolates from freshwater sources (n=16) and from patients (n=6) in Bangkok and surrounding areas...
  28. ncbi Four cases of Acanthamoeba keratitis treated with phototherapeutic keratectomy
    Michiko Kandori
    Department of Ophthalmology, Osaka University Medical School, Suita, Japan
    Cornea 29:1199-202. 2010
    To report 4 cases of Acanthamoeba keratitis treated with excimer laser phototherapeutic keratectomy (PTK) and to discuss the clinical efficacy of this procedure.
  29. ncbi Recovery of an environmental Chlamydia strain from activated sludge by co-cultivation with Acanthamoeba sp
    Astrid Collingro
    Division of Microbial Ecology, Institute for Ecology and Conservation Biology, University of Vienna, Althanstr 14, 1090 Vienna, Austria
    Microbiology 151:301-9. 2005
    ..reports the recovery of a novel environmental chlamydia strain from activated sludge by co-cultivation with Acanthamoeba sp...
  30. ncbi Amebic meningoencephalitides and keratitis: challenges in diagnosis and treatment
    Govinda S Visvesvara
    Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Curr Opin Infect Dis 23:590-4. 2010
    b>Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri, although free-living amebae, also cause devastating diseases in humans leading to death. Acanthamoeba spp. and B...
  31. pmc Pathogenesis of acanthamoeba keratitis
    Noorjahan Panjwani
    Departments of Ophthalmology and Biochemistry, The New England Eye Center, Tufts University School of Medicine, Boston, Massachusetts, USA
    Ocul Surf 8:70-9. 2010
    b>Acanthamoeba keratitis (AK) is a serious infection of the cornea. At present, diagnosis of the disease is not straightforward and treatment is very demanding...
  32. pmc Acanthamoeba keratitis in South India: a longitudinal analysis of epidemics
    Prajna Lalitha
    Aravind Eye Institute, Madurai, India
    Ophthalmic Epidemiol 19:111-5. 2012
    In light of the increased incidence of contact lens associated Acanthamoeba keratitis in recent years, this study analyzed longitudinal trends of its incidence among predominantly non-contact lens wearers in a high-volume referral center ..
  33. pmc Crescent bodies of Parachlamydia acanthamoeba and its life cycle within Acanthamoeba polyphaga: an electron micrograph study
    Gilbert Greub
    Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    Appl Environ Microbiol 68:3076-84. 2002
    ..The life cycle of Parachlamydia acanthamoeba within Acanthamoeba polyphaga was studied by transmission electron microscopy of 8-, 36-, and 144-h coculture...
  34. pmc Development of colorimetric microtiter plate assay for assessment of antimicrobials against Acanthamoeba
    James McBride
    Department of Immunology, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, 27 Taylor St, Glasgow, Scotland, UK
    J Clin Microbiol 43:629-34. 2005
    ..assay, based on the reduction of alamarBlue, to assess the efficacies of much needed new antimicrobials against Acanthamoeba species...
  35. pmc National outbreak of Acanthamoeba keratitis associated with use of a contact lens solution, United States
    Jennifer R Verani
    Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Emerg Infect Dis 15:1236-42. 2009
    An outbreak of Acanthamoeba keratitis, a rare, potentially blinding, corneal infection, was detected in the United States in 2007; cases had been increasing since 2004. A case-control study was conducted to investigate the outbreak...
  36. pmc Production of respirable vesicles containing live Legionella pneumophila cells by two Acanthamoeba spp
    S G Berk
    Center for the Management, Utilization and Protection of Water Resources, Tennessee Technological University, Cookeville 38505, USA
    Appl Environ Microbiol 64:279-86. 1998
    Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 microns in diameter and theoretically could contain several hundred bacteria. Viable L...
  37. ncbi Characterization and pathogenetic role of proteinase from Acanthamoeba castellanii
    B K Na
    Department of Biology, Chung-Ang University, Seoul, 156-756, Korea
    Microb Pathog 30:39-48. 2001
    A secreted proteinase was purified from the culture supernatant of Acanthamoeba castellanii with several chromatographic steps. The purified proteinase was a chymotrypsin-like serine proteinase...
  38. pmc Keratitis by Acanthamoeba triangularis: report of cases and characterization of isolates
    Ying Hua Xuan
    Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700 422, Korea
    Korean J Parasitol 46:157-64. 2008
    Three Acanthamoeba isolates (KA/E9, KA/E17, and KA/E23) from patients with keratitis were identified as Acanthamoeba triangularis by analysis of their molecular characteristics, a species not previously recognized to be a corneal pathogen...
  39. pmc Survival of environmental mycobacteria in Acanthamoeba polyphaga
    Toidi Adekambi
    Unite des Rickettsies, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Appl Environ Microbiol 72:5974-81. 2006
    ..to survive in the trophozoites and cysts of Acanthamoeba polyphaga. All the species tested entered the trophozoites of A...
  40. ncbi Laboratory investigation of Acanthamoeba lugdunensis from patients with keratitis
    Hak Sun Yu
    Department of Parasitology, Kyungbook National University School of Medicine, Taegu, South Korea
    Invest Ophthalmol Vis Sci 45:1418-26. 2004
    Species of four Acanthamoeba isolates (KA/E2, KA/E12, KA/E15, and KA/E16) from the cornea of patients with keratitis were identified and their molecular characteristics compared with those of other strains.
  41. ncbi Early diagnosis of amoebic keratitis due to a mixed infection with Acanthamoeba and Hartmannella
    Jacob Lorenzo-Morales
    University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda Astrofísico Fco Sánchez, S N, 38203, La Laguna, Tenerife, Canary Islands, Spain
    Parasitol Res 102:167-9. 2007
    A mixed keratitis due to Acanthamoeba and Hartmannella species is reported. The patient was a soft contact lens wearer. Early diagnosis was achieved by polymerase chain reaction and culture...
  42. ncbi Acanthamoeba interactions with human brain microvascular endothelial cells
    Selwa Alsam
    School of Biological and Chemical Sciences, Birkbeck College, University of London, WC1E 7HX, England, London, UK
    Microb Pathog 35:235-41. 2003
    b>Acanthamoeba are opportunistic protozoan parasites that can cause fatal granulomatous amoebic encephalitis, however, the pathogenic mechanisms associated with this disease remain unclear...
  43. ncbi Molecular cloning and characterization of a cDNA encoding a laminin-binding protein (AhLBP) from Acanthamoeba healyi
    Yeon Chul Hong
    Department of Parasitology, Kyungpook National University School of Medicine, Taegu 700 422, Republic of Korea
    Exp Parasitol 106:95-102. 2004
    Adherence of Acanthamoeba to host tissue is believed to be crucial in the establishment of amoebic keratitis or GAE...
  44. pmc Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence
    Won Tae Kim
    Department of Parasitology, Kyungpook National University School of Medicine, Daegu, Korea
    Korean J Parasitol 44:321-30. 2006
    The pathogenic mechanism of granulomatous amebic encephalitis (GAE) and amebic keratitis (AK) by Acanthamoeba has yet to be clarified. Protease has been recognized to play an important role in the pathogenesis of GAE and AK...
  45. ncbi Identification of Acanthamoeba genotype T4 and Paravahlkampfia sp. from two clinical samples
    Soykan Ozkoc
    Department of Parasitology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey
    J Med Microbiol 57:392-6. 2008
    In this study, two free-living amoebae strains, Acanthamoeba genotype T4 and Paravahlkampfia sp., which were isolated from keratitis cases are presented...
  46. ncbi Acanthamoeba interaction with extracellular matrix glycoproteins: biological and biochemical characterization and role in cytotoxicity and invasiveness
    Bruno da Rocha-Azevedo
    Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, 23298 0678, USA
    J Eukaryot Microbiol 56:270-8. 2009
    b>Acanthamoeba are free-living amoebae that are dispersed in most environments. Occasionally, Acanthamoeba cause serious human infections, such as keratitis and encephalitis...
  47. pmc Induction of morphological and electrophysiological changes in hamster cornea after in vitro interaction with trophozoites of Acanthamoeba spp
    Maritza Omaña-Molina
    Department of Experimental Pathology, Center for Research and Advanced Studies, Mexico City, Mexico
    Infect Immun 72:3245-51. 2004
    b>Acanthamoeba castellani and Acanthamoeba polyphaga are free-living amebae that cause keratitis and granulomatous encephalitis in humans...
  48. ncbi Elastase secretion in Acanthamoeba polyphaga
    Gabriela A Ferreira
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, CEP 05508 000 São Paulo, Brazil
    Acta Trop 112:156-63. 2009
    b>Acanthamoeba species are frequently isolated from soil and water collections. In the environment, the organisms multiply as phagotrophic trophozoites and encyst under adverse conditions...
  49. ncbi Serine-like proteolytic enzymes correlated with differential pathogenicity in patients with acute Acanthamoeba keratitis
    F R de Souza Carvalho
    Cornea and External Disease Service, Department of Ophthalmology, Federal University of Sao Paulo, Paulista School of Medicine, Sao Paulo, Brazil
    Clin Microbiol Infect 17:603-9. 2011
    Acute ocular infection due to free-living amoebae of the genus Acanthamoeba is characterized by severe pain, loss of corneal transparency and, eventually, blindness...
  50. ncbi The use of high-resolution ¹H nuclear magnetic resonance (NMR) spectroscopy in the clinical diagnosis of Acanthamoeba
    Sandra Hauber
    School of Biological and Chemical Sciences, Birkbeck College, University of London, London, UK
    Parasitol Res 109:1661-9. 2011
    b>Acanthamoeba are opportunistic protozoan pathogens that can produce sight-threatening keratitis and fatal granulomatous encephalitis. The successful prognosis requires early diagnosis and differentiation of pathogenic Acanthamoeba spp...
  51. pmc Contribution of amoebic coculture to recovery of Legionella isolates from respiratory samples: prospective analysis over a period of 32 months
    G Descours
    Universite de Lyon, Lyon, France
    J Clin Microbiol 50:1725-6. 2012
    ..1%. Amoebic coculture was particularly efficient in isolating Legionella spp. from respiratory samples contaminated with oropharyngeal flora...
  52. ncbi 'Candidatus Odyssella thessalonicensis' gen. nov., sp. nov., an obligate intracellular parasite of Acanthamoeba species
    R J Birtles
    Unite des Rickettsies, CNRS UPRESA 6020, Faculte de Medecine, Marseille, France
    Int J Syst Evol Microbiol 50:63-72. 2000
    An intracellular bacterium, strain L13, was observed infecting an environmental isolate of an Acanthamoeba species...
  53. ncbi Development and application of an in vitro susceptibility test for Acanthamoeba species isolated from keratitis to polyhexamethylene biguanide and chlorhexidine
    Sandhya Narasimhan
    Microbiology Research Center, Vision Research Foundation, Sankara Nehralaya, 18 College Road, Chennai 600 006, India
    Cornea 21:203-5. 2002
    To develop a reliable in vitro drug susceptibility test against Acanthamoeba isolates and to determine the minimum cysticidal concentration (MCC) of the drug.
  54. ncbi Treatment with voriconazole in 3 eyes with resistant Acanthamoeba keratitis
    Stacy Bang
    Cataract, Cornea and External Disease Service, Wilmer Eye Institute, Johns Hopkins Hospital, Baltimore, Maryland, USA
    Am J Ophthalmol 149:66-9. 2010
    ..To report the use of topical voriconazole 1% (Vfend; Pfizer Inc, New York, New York, USA) ophthalmic solution for Acanthamoeba keratitis (AK) resistant to treatment with chlorhexidine (PerioChip; Dexel Pharma Technologies, Jerusalem, ..
  55. ncbi Anti-acanthamoeba efficacy and toxicity of miltefosine in an organotypic skin equivalent
    Julia Walochnik
    Department of Medical Parasitology, Institute of Hygiene, Medical University of Vienna, 1095 Vienna, Austria
    J Antimicrob Chemother 64:539-45. 2009
    Acanthamoebae can cause infections of several organs, including eye, skin, lung and brain. Except for Acanthamoeba keratitis, these infections are linked to immunodeficiency...
  56. ncbi Illuminating the evolutionary history of chlamydiae
    Matthias Horn
    Department of Microbial Ecology, Institute of Ecology and Conservation Biology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria
    Science 304:728-30. 2004
    ..Ancient chlamydiae appear to be the originators of mechanisms for the exploitation of eukaryotic cells...
  57. pmc Infection of Acanthamoeba polyphaga with Simkania negevensis and S. negevensis survival within amoebal cysts
    S Kahane
    Department of Virology, Faculty of Health Sciences, Ben Gurion University of the Negev, Israel
    Appl Environ Microbiol 67:4789-95. 2001
    ..This study reports for the first time the ability of S. negevensis to survive and grow inside Acanthamoeba polyphaga in addition to its known ability to grow in cell cultures of human or simian origin. Infectivity of S...
  58. pmc In situ detection of novel bacterial endosymbionts of Acanthamoeba spp. phylogenetically related to members of the order Rickettsiales
    T R Fritsche
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
    Appl Environ Microbiol 65:206-12. 1999
    ..We report here the isolation from corneal scrapings of two Acanthamoeba strains that harbor gram-negative rod endosymbionts that could not be cultured by standard techniques...
  59. pmc The Legionella pneumophila rpoS gene is required for growth within Acanthamoeba castellanii
    L M Hales
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Bacteriol 181:4879-89. 1999
    ..strain was able to kill HL-60- and THP-1-derived macrophages, it could not replicate within a protozoan host, Acanthamoeba castellanii. These data suggest that L...
  60. pmc Survival and growth of Francisella tularensis in Acanthamoeba castellanii
    Hadi Abd
    Swedish Defence Research Agency, SE 901 82 Umea, Sweden
    Appl Environ Microbiol 69:600-6. 2003
    ..To test this hypothesis, Acanthamoeba castellanii was cocultured with a strain of F...
  61. ncbi Contact lens-related microbial keratitis: recent outbreaks
    Avni Patel
    Wills Eye, Philadelphia, Pennsylvania 19107, USA
    Curr Opin Ophthalmol 19:302-6. 2008
    ..This paper reviews the literature generated on atypical contact lens-related infections and their relationship with particular contact lens solutions since 2005...
  62. pmc Intracellular growth in Acanthamoeba castellanii affects monocyte entry mechanisms and enhances virulence of Legionella pneumophila
    J D Cirillo
    Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Lincoln, Nebraska 68583, USA
    Infect Immun 67:4427-34. 1999
    ..L. pneumophila grown in one of its environmental hosts, Acanthamoeba castellanii, is phenotypically different from L. pneumophila grown on standard laboratory medium (BCYE agar)...
  63. ncbi Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality
    Vincent Thomas
    STERIS SA R and D, Fontenay aux Roses, France
    FEMS Microbiol Rev 34:231-59. 2010
    ..by listing all known pathogenic microbial species for which growth and/or survival promotion by FLA (mainly Acanthamoeba spp.) has been demonstrated...
  64. pmc Infection of Acanthamoeba castellanii with Mycobacterium bovis and M. bovis BCG and survival of M. bovis within the amoebae
    Stephanie J Taylor
    Microbial Sciences Group, School of Biomedical and Life Sciences, University of Surrey, Guildford, Surrey GU2 7XH, United Kingdom
    Appl Environ Microbiol 69:4316-9. 2003
    ..We have shown that M. bovis survived ingestion by Acanthamoeba castellanii. In contrast, two strains of M. bovis BCG did not survive well within Acanthamoeba.
  65. pmc Surviving within the amoebal exocyst: the Mycobacterium avium complex paradigm
    Iskandar Ben Salah
    Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS 6236, IRD 189, IFR 48 Faculté de Médecine, Universite de la Mediterranee, Marseille France
    BMC Microbiol 10:99. 2010
    ..We herein studied the intra-ameobal location of all members of the MAC as model organisms for environmental mycobacteria...
  66. pmc Cryptococcus neoformans interactions with amoebae suggest an explanation for its virulence and intracellular pathogenic strategy in macrophages
    J N Steenbergen
    Department of Microbiology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 98:15245-50. 2001
    ..and amoebae killing assays, and phagocytosis assays revealed that Cn is phagocytosed by and replicates in Acanthamoeba castellanii, which leads to death of amoebae...
  67. pmc Viability of Acanthamoeba after exposure to a multipurpose disinfecting contact lens solution and two hydrogen peroxide systems
    K Hiti
    Department of Ophthalmology, University of Graz, Austria
    Br J Ophthalmol 86:144-6. 2002
    Contact lens cases contaminated with Acanthamoeba are a major risk factor for an infection of the eye. In this study the anti-Acanthamoeba activity of three different contact lens storage solutions was tested.
  68. ncbi Acanthamoeba polyphaga is a possible host for Vibrio cholerae in aquatic environments
    Gunnar Sandström
    Centre for Microbiological Preparedness, Swedish Institute for Infections Disease Control, SE 17182 Solna, Sweden
    Exp Parasitol 126:65-8. 2010
    b>Acanthamoeba is a genus of free-living amoebae found to be able to host many bacterial species living in the environment. Acanthamoebae and Vibrio cholerae are found in the aquatic environments of cholera endemic areas...
  69. ncbi Host cell processes that influence the intracellular survival of Legionella pneumophila
    Sunny Shin
    Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Avenue, Room 345, New Haven, CT 06536, USA
    Cell Microbiol 10:1209-20. 2008
    ..In this review, we discuss how L. pneumophila manipulates host cells, as well as host cell processes that either facilitate or impede its intracellular survival...
  70. pmc Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus
    Nathan Zauberman
    Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot, Israel
    PLoS Biol 6:e114. 2008
    ..Here we present an exception to this paradigm by demonstrating that genome delivery and packaging in the virus Acanthamoeba polyphaga mimivirus occur through two distinct portals...
  71. pmc Diversity of bacterial endosymbionts of environmental acanthamoeba isolates
    Stephan Schmitz-Esser
    Department of Microbial Ecology, University of Vienna, Althanstr 14, 1090 Vienna, Austria
    Appl Environ Microbiol 74:5822-31. 2008
    ..In this study, the symbionts of eight novel environmental Acanthamoeba strains isolated from different locations worldwide were characterized...
  72. ncbi Results of penetrating keratoplasty for visual rehabilitation after Acanthamoeba keratitis
    Shady T Awwad
    Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 9057, USA
    Am J Ophthalmol 140:1080-1084. 2005
    To assess the results of penetrating keratoplasty in quiet eyes after resolution of Acanthamoeba keratitis.
  73. ncbi [Use of the presence of cellulose in cellular wall of Acanthamoeba cysts for diagnostic purposes]
    Monika Derda
    Katedra i Zakład Biologii i Parazytologii Lekarskiej, Uniwersytet Medyczny im Karola Marcinkowskiego, ul Fredry 10, 61 701 Poznan
    Wiad Parazytol 55:47-51. 2009
    Species identification within the genus Acanthamoeba is based predominantly on morphological and biochemical features. It is labor-intensive and requires cloning and axenization...
  74. pmc Detection limits of Legionella pneumophila in environmental samples after co-culture with Acanthamoeba polyphaga
    Lisa Conza
    Swiss National Reference Centre for Legionella, Cantonal Institute of Microbiology, Via Mirasole 22A, 6500, Bellinzona, Switzerland
    BMC Microbiol 13:49. 2013
    The efficiency of recovery and the detection limit of Legionella after co-culture with Acanthamoeba polyphaga are not known and so far no investigations have been carried out to determine the efficiency of the recovery of Legionella spp...
  75. pmc Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients
    Ying Hua Xuan
    Department of Parasitology, Kyungpook National University School of Medicine, Taegu 700 422, Korea
    Korean J Parasitol 45:1-9. 2007
    The endosymbionts of 4 strains of Acanthamoeba (KA/E9, KA/E21, KA/E22, and KA/E23) isolated from the infected corneas of Korean patients were characterized via orcein stain, transmission electron microscopic examination, and 16S rDNA ..
  76. ncbi Differential stimulation of microglial pro-inflammatory cytokines by Acanthamoeba culbertsoni versus Acanthamoeba castellanii
    Francine Marciano-Cabral
    Department of Microbiology and Immunology, Virginia Commonwealth University School of Medicine, Richmond, VA 23298 0678, USA
    J Eukaryot Microbiol 51:472-9. 2004
    b>Acanthamoeba spp. are opportunistic pathogens that cause granulomatous amebic encephalitis. We compared the highly pathogenic species A. culbertsoni to the relatively less pathogenic species A...
  77. ncbi Advantages of using mitochondrial 16S rDNA sequences to classify clinical isolates of Acanthamoeba
    Dolena R Ledee
    Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210 1292, USA
    Invest Ophthalmol Vis Sci 44:1142-9. 2003
    This work was intended to test the classification of Acanthamoeba into genotypes based on nuclear ribosomal RNA gene (18S rDNA, Rns) sequences. Nearly all Acanthamoeba keratitis (AK) isolates are genotype RnsT4...
  78. ncbi Granulomatous inflammation in Acanthamoeba keratitis: an immunohistochemical study of five cases and review of literature
    G K Vemuganti
    Department of Ophthalmic Pathology Service, L V Prasad Eye Institute, L V Prasad Marg, Banjara Hills, Hyderabad 500 034, India
    Indian J Med Microbiol 23:231-8. 2005
    b>Acanthamoeba keratitis usually presents as a necrotizing stromal inflammation. We report a rare presentation of granulomatous inflammation in Acanthamoeba keratitis
  79. ncbi Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates
    Amir Hossein Maghsood
    School of Biological and Chemical Sciences, Birkbeck College, University of London, London WC1E 7HX, UK
    J Med Microbiol 54:755-9. 2005
    The majority of the keratitis-causing Acanthamoeba isolates are genotype T4...
  80. ncbi ITS1 sequence variabilities correlate with 18S rDNA sequence types in the genus Acanthamoeba (Protozoa: Amoebozoa)
    Martina Köhsler
    Department of Medical Parasitology, Clinical Institute of Hygiene and Medical Microbiology, Medical University of Vienna, Kinderspitalgasse 15, 1095 Vienna, Austria
    Parasitol Res 98:86-93. 2006
    ..spacer (ITS1), a more variable region than the 18S rRNA gene, was sequenced, and the sequences of 15 different Acanthamoeba isolates were compared to reveal if ITS1 sequence variability correlates with 18S rDNA sequence typing and if ..
  81. ncbi Detection of free living amoebae, Acanthamoeba and Naegleria, in swimming pools, Malaysia
    I Init
    Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
    Trop Biomed 27:566-77. 2010
    This study reports the detection of Acanthamoeba and Naegleria species in 14 swimming pools around Petaling Jaya and Kuala Lumpur, Malaysia...
  82. ncbi Acanthamoeba sp. promotes the survival and growth of Acinetobacter baumanii
    Estelle Cateau
    Laboratoire de parasitologie et mycologie médicale, Faculte de Medecine et de Pharmacie, Poitiers, France
    FEMS Microbiol Lett 319:19-25. 2011
    ..In this paper, we have studied potential relationships between A. baumanii and Acanthamoeba species. Two strains of A...
  83. ncbi Acanthamoeba castellanii: structural basis of the cytopathic mechanisms
    Arturo González-Robles
    Department of Experimental Pathology, Center for Research and Advanced Studies, Mexico City, Mexico
    Exp Parasitol 114:133-40. 2006
    In this study we report observations on the structural mechanisms of the cytopathic effect of Acanthamoeba castellanii trophozoites on cultured MDCK cell monolayers. Co-incubations were carried out for a maximum of 24h...
  84. ncbi Acanthamoeba meningoencephalitis in an immunocompetent patient: an autopsy case report
    Rajesh Reddy
    Department of Neurosurgery, Nizam s Institute of Medical Sciences, Hyderabad, India
    Neuropathology 31:183-7. 2011
    ..We report a rare, fatal case of granulomatous amoebic encephalitis in an immunocompetent patient mimicking CNS tuberculosis, and review the imageological features and diagnostic tests...
  85. ncbi Ecto-ATPases of clinical and non-clinical isolates of Acanthamoeba
    James Sissons
    School of Biological and Chemical Sciences, Birkbeck College, University of London, London WC1E 7HX, UK
    Microb Pathog 37:231-9. 2004
    b>Acanthamoeba are opportunistic protozoan parasites that can cause fatal granulomatous amoebic encephalitis and eye keratitis, however the pathogenic mechanisms of Acanthamoeba remain unclear...
  86. ncbi Factors affecting the epidemiology of Acanthamoeba keratitis
    Youhanna W Ibrahim
    Department of Ophthalmology, Queen Alexandra Hospital, Portsmouth, United Kingdom
    Ophthalmic Epidemiol 14:53-60. 2007
    Despite being a relatively rare disease in comparison with other forms of infectious keratitis, Acanthamoeba keratitis is a potentially blinding disease...
  87. ncbi Biocides currently used for bronchoscope decontamination are poorly effective against free-living amoebae
    Gilbert Greub
    Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    Infect Control Hosp Epidemiol 24:784-6. 2003
    ..We showed that two particular biocides are inadequate for disinfecting bronchoscopes, as Acanthamoeba polyphaga survived 3 hours of exposure to these undiluted agents.
  88. ncbi Molecular and physiological differentiation between pathogenic and nonpathogenic Acanthamoeba
    Naveed A Khan
    Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Curr Microbiol 45:197-202. 2002
    In this study, 14 isolates of Acanthamoeba from both clinical and environmental sources belonging to seven different species were assayed for tolerance of high osmotic pressure, temperature tolerance, extracellular proteases, and ..
  89. ncbi The role of the innate and adaptive immune responses in Acanthamoeba keratitis
    Jerry Y Niederkorn
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75390 9057, USA
    Arch Immunol Ther Exp (Warsz) 50:53-9. 2002
    ..Free-living amoebae of the genus Acanthamoeba produce a progressive infection of the cornea called Acanthamoeba keratitis...
  90. ncbi The response regulator LetA regulates the stationary-phase stress response in Legionella pneumophila and is required for efficient infection of Acanthamoeba castellanii
    Damien Lynch
    Department of Medical Microbiology and Hygiene, University of Ulm, Robert Koch Str 8, D 89081, Ulm, Germany
    FEMS Microbiol Lett 219:241-8. 2003
    ..The letA mutant exhibited reduced infectivity and was defective for intracellular growth within Acanthamoeba castellanii. Transcription of the rpoS and dotA genes was reduced in the letA mutant...
  91. pmc Endosymbionts of Acanthamoeba isolated from domestic tap water in Korea
    Seon Hee Choi
    Department of Parasitology, School of Medicine, Pusan National University, Busan, 602 739, Korea
    Korean J Parasitol 47:337-44. 2009
    In a previous study, we reported our discovery of Acanthamoeba contamination in domestic tap water; in that study, we determined that some Acanthamoeba strains harbor endosymbiotic bacteria, via our molecular characterization by ..
  92. ncbi Acanthamoeba encephalitis
    V Kaushal
    Department of Microbiology, Dayanand Medical College and Hospital, Ludhiana 141 001, Punjab, India
    Indian J Med Microbiol 26:182-4. 2008
    Central nervous system infection with free-living amoebae is rare. We present a fatal case of Acanthamoeba encephalitis in a 63-year-old female from India where acanthamoebae were demonstrated and cultured from CSF...
  93. ncbi Intracellular localization and dynamics of myosin-II and myosin-IC in live Acanthamoeba by transient transfection of EGFP fusion proteins
    Hyun Hee Kong
    Structural Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    J Cell Sci 115:4993-5002. 2002
    We developed a reliable method for transient transfection of Acanthamoeba using Superfect (Qiagen) and a vector with the Acanthamoeba ubiquitin promoter and enhanced green fluorescent protein (EGFP) as the reporter gene...
  94. ncbi Cure of Acanthamoeba cerebral abscess in a liver transplant patient
    Konrad Tang Tat Fung
    Liver Transplantation and Hepatobiliary Medicine, Royal Free Hospital, London, United Kingdom
    Liver Transpl 14:308-12. 2008
    b>Acanthamoeba-related cerebral abscess and encephalitis are rare but usually fatal, being caused by free-living amoebic infections usually occurring in immunocompromised patients...
  95. pmc Cytopathic changes in rat microglial cells induced by pathogenic Acanthamoeba culbertsoni: morphology and cytokine release
    H J Shin
    Department of Microbiology, Ajou University School of Medicine, Suwon 442 749, Korea
    Clin Diagn Lab Immunol 8:837-40. 2001
    To determine whether pathogenic Acanthamoeba culbertsoni trophozoites and lysate can induce cytopathic changes in primary-culture microglial cells, morphological changes were observed by transmission electron microscopy (TEM)...
  96. ncbi Successful treatment of Acanthamoeba meningitis with combination oral antimicrobials
    T Singhal
    Department of Pediatrics, All India Institute of Medical Sciences, New Delhi
    Pediatr Infect Dis J 20:623-7. 2001
    b>Acanthamoeba was implicated as the causative agent of chronic meningitis in three apparently immunocompetent children. Diagnosis was established by cerebrospinal fluid wet mount examination and culture...
  97. doi Successful treatment of acanthamoeba meningoencephalitis during induction therapy of childhood acute lymphoblastic leukemia
    Deepak Gupta
    Pediatr Blood Cancer 50:1292-3. 2008
  98. pmc Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene
    H S Yu
    Department of Parasitology, Kyungpook National University School of Medicine, Taegu, Korea
    Korean J Parasitol 37:181-8. 1999
    ..We investigated the value of mitochondrial small subunit rRNA gene (mt SSU rDNA) PCR-RFLP as a taxonomic tool for Acanthamoeba isolates with close inter-relationships...
  99. pmc Cytotoxic activity of N-chlorotaurine on Acanthamoeba spp
    Ursula Fürnkranz
    Department of Medical Parasitology, Clinical Institute of Hygiene and Medical Microbiology, Medical University of Vienna, Kinderspitalgasse 15, Vienna A 1095, Austria
    Antimicrob Agents Chemother 52:470-6. 2008
    b>Acanthamoeba spp...
  100. pmc Antibiotic susceptibilities of Parachlamydia acanthamoeba in amoebae
    M Maurin
    Unite des Rickettsies, CNRS UPRES A 6020, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 46:3065-7. 2002
    Parachlamydia acanthamoeba are intracellular bacteria of amoebae and are considered potential etiological agents of human pneumonia...
  101. ncbi Keratocyte loss in Acanthamoeba keratitis: phagocytosis, necrosis or apoptosis?
    G K Vemuganti
    Ophthalmic Pathology Service, L V Prasad Eye Institute, L V Prasad Marg, Banjara Hills, Hyderabad 500 034, India
    Indian J Ophthalmol 48:291-4. 2000
    Pathogenesis of Acanthamoeba keratitis involves breakdown of epithelial barrier, stromal invasion by Acanthamoeba, loss of keratocytes, inflammatory response and finally stromal necrosis...

Research Grants44

  1. CRISPR Cas genes and Legionella pneumophila infection
    Nicholas P Cianciotto; Fiscal Year: 2013
    ..Furthermore, loss of cas2 results in a dramatic (approx. 1000-fold) defect in Lpn infection of the amoeba Acanthamoeba castellanii...
  2. New Therapies for Pathogenic Free-Living Amoebae
    Dennis E Kyle; Fiscal Year: 2013
    ..Naegleria fowleri and Acanthamoeba spp. are the best known examples of pathogenic FLA. N...
  3. PATHOGENESIS OF ACANTHAMOEBA KERATITIS
    Noorjahan Panjwani; Fiscal Year: 2013
    Abstract: Acanthamoeba keratitis (AK) is a serious, debilitating, and intensely painful infection of the cornea caused by parasites of the genus Acanthamoeba...
  4. Planning Grant for Parasitic Ulcer Treatment Trial
    Jeremy David Keenan; Fiscal Year: 2013
    ..The objective of this study is to plan for a clinical trial to determine better ways to diagnose and treat acanthamoeba keratitis...
  5. Biology, Immunology &therapy of Acanthamoeba Keratitis
    Hassan Alizadeh; Fiscal Year: 2013
    Summary Acanthamoeba keratitis is a sight-threatening corneal disease caused by pathogenic free-living amoebae...
  6. GENETIC DETERMINANTS OF PATHOGENICITY IN LEGIONELLA
    Lucy Tompkins; Fiscal Year: 2001
    ..Other studies demonstrated that prior growth of laboratory passaged strains in Acanthamoeba enhanced the invasiveness of L. pneumophila into macrophages...
  7. NEW TREATMENT FOR AMOEBIC KERATITIS
    CLARENCE VILLEMEZ; Fiscal Year: 2001
    ..Unlike disseminated Acanthamoeba infections, which are life threatening and occur in patients with compromised immune systems, acanthamoebic ..
  8. THREE DIMENSIONAL STRUCTURE OF ALPHA ACTININ
    Kenneth Taylor; Fiscal Year: 2000
    ..3) Crystallize Acanthamoeba alpha-actinin and obtain an image that reveals the alignment of protein domains...
  9. RIBOSOMAL RNA TRANSCRIPTION FACTOR FUNCTIONAL ANALYSIS
    Marvin Paule; Fiscal Year: 1992
    ..The role of the core is to bind one or more trans-acting transcription factors. In Acanthamoeba, a single factor, TIF, binds to a defined sequence in the core...
  10. PATHOGENESIS AND THERAPY OF ACANTHAMOEBA KERATITIS
    Michael Osato; Fiscal Year: 1990
    b>Acanthamoeba keratitis is an infrequent, progressive, and often severe infection of healthy young adults, predominantly occurring as a complication of soft contact lens wear or minor corneal injury...
  11. INVASION GENE AND VIRULENCE OF LEGIONELLA
    Jeffrey Cirillo; Fiscal Year: 2001
    ..The investigator's recent data indicate that Legionella grown in an environmental host, the amoebae Acanthamoeba castellani, are approximately two to four logs more invasive for host cells than bacteria grown in the standard ..
  12. RNA POLYMERASE III TRANSCRIPTION REGULATION MECHANISM
    Marvin Paule; Fiscal Year: 1999
    ..In Acanthamoeba, rRNA transcription is regulated by polymerase I modification, and recent results show that 5S RNA ..
  13. ANTI-ACANTHAMOEBA EYE INFECTION DROPS
    Francis Smith; Fiscal Year: 2000
    ..of this project is to develop an eye drop which is more effective than other existing therapies in treating Acanthamoeba keratitis...
  14. TRANSCRIPTION OF ACANTHAMOEBA RNA PLYMERASE II GENES
    Erik Bateman; Fiscal Year: 2004
    DESCRIPTION (Adapted from the Applicant's Abstract): Acanthamoeba castellanii is a small, free living amoeba that is the causative agent for Acanthamoeba keratitis...
  15. Mining slime mold for susceptibility genes to fungi
    Arturo Casadevall; Fiscal Year: 2005
    ..that the interactions of the human pathogenic fungus Cryptococcus neoformans with the ameboid predators Acanthamoeba castellani and Dictostylium discoideum are very similar to that observed with mammalian macrophages...
  16. METABOLIC ENHANCEMENT OF CONTACT LENS PRESERVATIVES
    Francis Smith; Fiscal Year: 1999
    DESCRIPTION: Current contact lens care systems are not very effective against Acanthamoeba because the cyst form of the microorganism is more resistant the trophozoite form to the preservatives in the disinfecting solutions...
  17. TOWARD A PROTOZOAN IMMUNOTOXIN
    CLARENCE VILLEMEZ; Fiscal Year: 1991
    ..proposed here is designed to answer that question by preparing immunotoxins that react with essentially all Acanthamoeba castellanii cell surface antigens, and evaluating them for cell killing potential...
  18. EFFECTS OF LEAD ON PROCARYOTIC AND EUCARYOTIC CELLS
    R Skogerboe; Fiscal Year: 1980
    ..properties of the cytosol and membranal ribosomal contents of bacteria and the eukaryotic organisms Acanthamoeba, Tetrahymena, and Dunaliella exposed to lead to further test the effectiveness of lead in the alteration of the ..
  19. DEVELOPMENT OF DNA PROBES FOR ACANTHAMOEBAS
    SUSAN GOSS; Fiscal Year: 1992
    DESCRIPTION (Adapted from the Applicant's Abstract): Acanthamoeba species are free-living amoebas that are universally distributed in the environment...
  20. Fluorescence Lifetime and Steady-State Spectrometer
    E Ostap; Fiscal Year: 2003
    ..The core user group has extensive training in the operation, design, and maintenance of spectroscopic equipment. ..
  21. Optimization of Vectors for Stable Transfection of Acanthamoeba
    Erik Bateman; Fiscal Year: 2008
    unreadable] DESCRIPTION (provided by applicant): Acanthamoeba castellanii is one of several closely related species that cause the eye disease Acanthamoeba keratitis...
  22. Ophthalmic Antibiotic Resistance Study
    Kirk Wilhelmus; Fiscal Year: 2003
    ..abstract_text> ..
  23. DNA Probes for Acanthamoeba Genomics and Epidemiology
    Paul Fuerst; Fiscal Year: 2007
    ..study extends previous investigations of DNA diagnostic approaches for the opportunistically pathogenic protist Acanthamoeba, the causative agent of the sight-threatening disease Acanthamoeba keratitis (AK)...
  24. Corneal Refractive Power Post Refractive Surgery
    CHARLOTTE JOSLIN; Fiscal Year: 2008
    ..The long-term objective to this research is to contribute to the knowledge of IOL power determination in post-refractive surgery patients. ..
  25. Functional Relevance of Microglial Cannabinoid Receptors
    Guy Cabral; Fiscal Year: 2004
    ..Functional assays will employ paired cannabinoid enantiomers, receptor subtype-specific antagonists, and cannabinoids exhibiting differential ligand binding affinities to establish structure-activity relationships. ..
  26. CHEMOKINE RECEPTORS AND SIV NEUROPATHOGENESIS
    Susan Westmoreland; Fiscal Year: 2003
    ..We will then assess neuronal cultures for cellular injury and induction of apoptosis. ..
  27. Sixth International Conference on Legionella
    Nicholas Cianciotto; Fiscal Year: 2005
    ..This unique gathering of microbiologists, physicians, public health officials, and engineers is the definitive meeting for those concerned with Legionella and its associated diseases. ..
  28. The Role of Selectin-Mediated Recognition in Glaucoma
    Noorjahan Panjwani; Fiscal Year: 2006
    ..The proposed studies will determine whether the selectin-based carbohydrate recognition system plays a role in the pathogenic mechanisms of aqueous outflow resistance in glaucoma. ..
  29. Control of Eye Melanoma Metastsis
    Hans E Grossniklaus; Fiscal Year: 2010
    ..The third aim determines the mechanism of combined angiostatin/IFN control of the micrometastases using methods in the first two aims. ..
  30. Molecular pathogenesis of Francisella tularnesis
    YOUSEF A ABU KWAIK; Fiscal Year: 2010
    ..The bacterial effectors involved in exploiting the macrophage are potential targets for treatment as well as potential vaccine candidates. ..
  31. Regulatory Role of CTL-O2/Bcl2 in Epithelial Homeostasis
    H Cavanagh; Fiscal Year: 2006
    ..abstract_text> ..
  32. CORE GRANT FOR VISION RESEARCH
    Noorjahan Panjwani; Fiscal Year: 2005
    ..abstract_text> ..
  33. The Pore-forming Toxin of Legionella Pneumophila
    Yousef Abu Kwaik; Fiscal Year: 2003
    ..Our studies on L. pneumophila may provide a paradigm for other vacuolar intracellular pathogens such as Mycobacterium, Salmonella, and Chlamydia. ..
  34. Receptor-Mediated Endocytosis of Legionella Pneumophila
    Yousef Abu Kwaik; Fiscal Year: 2004
    ..Our studies may uncover potential pathogenic evolution of Lpn to invade the more evolved mammalian cells, and may contribute to the understanding of invasion of protozoa by Mycobacterium and Chlamydia. ..
  35. SUBMACULAR SURGERY TRIALS PATHOLOGY CENTER
    Hans Grossniklaus; Fiscal Year: 2003
    ..Additionally, eyes obtained post-mortem from patients enrolled in the SST will be studied for pathologic correlation with clinical findings and outcomes. ..
  36. Control of Eye Melanoma Metastasis
    Hans Grossniklaus; Fiscal Year: 2003
    ..Both the IFN and angiostatin will prevent the progression of micrometastases into metastases by anti-angiogenesis. Angiostatin will prevent metastases by an anti-melanoma invasion effect. ..