trypanosoma cruzi

Summary

Summary: The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors.

Top Publications

  1. ncbi Chagas disease
    Anis Rassi
    Division of Cardiology, Anis Rassi Hospital, Goiania, GO, Brazil
    Lancet 375:1388-402. 2010
  2. ncbi A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI
    B Zingales
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, SP, Brasil
    Mem Inst Oswaldo Cruz 104:1051-4. 2009
  3. ncbi The revised Trypanosoma cruzi subspecific nomenclature: rationale, epidemiological relevance and research applications
    Bianca Zingales
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Avenida Professor Lineu Prestes 748, 05508 000 Sao Paulo, SP, Brazil
    Infect Genet Evol 12:240-53. 2012
  4. pmc Trypanosoma cruzi infection induces a global host cell response in cardiomyocytes
    Patricio A Manque
    Department of Microbiology, Virginia Commonwealth University, Richmond, VA, USA
    Infect Immun 79:1855-62. 2011
  5. pmc Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi
    C A Machado
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 98:7396-401. 2001
  6. pmc International study to evaluate PCR methods for detection of Trypanosoma cruzi DNA in blood samples from Chagas disease patients
    Alejandro G Schijman
    Laboratorio de Biología Molecular de Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    PLoS Negl Trop Dis 5:e931. 2011
  7. ncbi Comparative phylogeography of Trypanosoma cruzi TCIIc: new hosts, association with terrestrial ecotopes, and spatial clustering
    Arlei Marcili
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, 05508 900 Sao Paulo, Brazil
    Infect Genet Evol 9:1265-74. 2009
  8. pmc Chromosome level assembly of the hybrid Trypanosoma cruzi genome
    D Brent Weatherly
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA
    BMC Genomics 10:255. 2009
  9. ncbi Pathogenesis of chronic Chagas heart disease
    Jose Antonio Marin-Neto
    Cardiology Division, Department of Internal Medicine, Medical School of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil
    Circulation 115:1109-23. 2007
  10. ncbi Interactions between intestinal compounds of triatomines and Trypanosoma cruzi
    Eloi S Garcia
    Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Manguinhos, CEP, Rio de Janeiro, Brazil
    Trends Parasitol 26:499-505. 2010

Detail Information

Publications342 found, 100 shown here

  1. ncbi Chagas disease
    Anis Rassi
    Division of Cardiology, Anis Rassi Hospital, Goiania, GO, Brazil
    Lancet 375:1388-402. 2010
    Chagas disease is a chronic, systemic, parasitic infection caused by the protozoan Trypanosoma cruzi, and was discovered in 1909...
  2. ncbi A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI
    B Zingales
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, SP, Brasil
    Mem Inst Oswaldo Cruz 104:1051-4. 2009
    In an effort to unify the nomenclature of Trypanosoma cruzi, the causative agent of Chagas disease, an updated system was agreed upon at the Second Satellite Meeting. A consensus was reached that T...
  3. ncbi The revised Trypanosoma cruzi subspecific nomenclature: rationale, epidemiological relevance and research applications
    Bianca Zingales
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Avenida Professor Lineu Prestes 748, 05508 000 Sao Paulo, SP, Brazil
    Infect Genet Evol 12:240-53. 2012
    The protozoan Trypanosoma cruzi, its mammalian reservoirs, and vectors have existed in nature for millions of years. The human infection, named Chagas disease, is a major public health problem for Latin America. T...
  4. pmc Trypanosoma cruzi infection induces a global host cell response in cardiomyocytes
    Patricio A Manque
    Department of Microbiology, Virginia Commonwealth University, Richmond, VA, USA
    Infect Immun 79:1855-62. 2011
    Chagas' disease, caused by the hemoflagellate protozoan Trypanosoma cruzi, affects millions of people in South and Central America...
  5. pmc Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi
    C A Machado
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 98:7396-401. 2001
    ..a large data set of nucleotide sequences from two nuclear genes and a region of the mitochondrial genome of Trypanosoma cruzi, the agent of Chagas' disease...
  6. pmc International study to evaluate PCR methods for detection of Trypanosoma cruzi DNA in blood samples from Chagas disease patients
    Alejandro G Schijman
    Laboratorio de Biología Molecular de Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    PLoS Negl Trop Dis 5:e931. 2011
    ..The purpose of this study was to evaluate the performance of PCR methods in detection of Trypanosoma cruzi DNA by an external quality evaluation.
  7. ncbi Comparative phylogeography of Trypanosoma cruzi TCIIc: new hosts, association with terrestrial ecotopes, and spatial clustering
    Arlei Marcili
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, 05508 900 Sao Paulo, Brazil
    Infect Genet Evol 9:1265-74. 2009
    We characterized 28 new isolates of Trypanosoma cruzi IIc (TCIIc) of mammals and triatomines from Northern to Southern Brazil, confirming the widespread distribution of this lineage...
  8. pmc Chromosome level assembly of the hybrid Trypanosoma cruzi genome
    D Brent Weatherly
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA
    BMC Genomics 10:255. 2009
    ..genome sequences of the kinetoplastid pathogens Leishmania major and Trypanosoma brucei the assembly of the Trypanosoma cruzi genome has been hindered by its repetitive nature and the fact that the reference strain (CL Brener) is a ..
  9. ncbi Pathogenesis of chronic Chagas heart disease
    Jose Antonio Marin-Neto
    Cardiology Division, Department of Internal Medicine, Medical School of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil
    Circulation 115:1109-23. 2007
    ..Despite nearly 1 century of research, the pathogenesis of chronic Chagas cardiomyopathy is incompletely understood, the most intriguing challenge of which is the complex host-parasite interaction...
  10. ncbi Interactions between intestinal compounds of triatomines and Trypanosoma cruzi
    Eloi S Garcia
    Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Manguinhos, CEP, Rio de Janeiro, Brazil
    Trends Parasitol 26:499-505. 2010
    Triatomine bugs are vectors of Trypanosoma cruzi, the etiologic agent of Chagas disease, a devastating disease that disables and leads to the death of many people in Latin America...
  11. ncbi Myeloid-derived suppressor cells infiltrate the heart in acute Trypanosoma cruzi infection
    Henar Cuervo
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Immunol 187:2656-65. 2011
    Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, affects several million people in Latin America...
  12. ncbi NADPH oxidase inhibition ameliorates Trypanosoma cruzi-induced myocarditis during Chagas disease
    Monisha Dhiman
    Department of Microbiology and Immunology, Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555 1070, USA
    J Pathol 225:583-96. 2011
    b>Trypanosoma cruzi, the aetiological agent of Chagas disease, invades nucleated mammalian cells including macrophages. In this study, we investigated the crosstalk between T...
  13. pmc Trypanosoma cruzi subverts the sphingomyelinase-mediated plasma membrane repair pathway for cell invasion
    Maria Cecilia Fernandes
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
    J Exp Med 208:909-21. 2011
    Upon host cell contact, the protozoan parasite Trypanosoma cruzi triggers cytosolic Ca(2+) transients that induce exocytosis of lysosomes, a process required for cell invasion...
  14. pmc Flow cytometric analysis and microsatellite genotyping reveal extensive DNA content variation in Trypanosoma cruzi populations and expose contrasts between natural and experimental hybrids
    Michael D Lewis
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    Int J Parasitol 39:1305-17. 2009
    b>Trypanosoma cruzi exhibits remarkable genetic heterogeneity. This is evident at the nucleotide level but also structurally, in the form of karyotypic variation and DNA content differences between strains...
  15. pmc Sex, subdivision, and domestic dispersal of Trypanosoma cruzi lineage I in southern Ecuador
    Sofía Ocaña-Mayorga
    Centro de Investigación en Enfermedades Infecciosas, Pontificia Universidad Catolica del Ecuador, Quito, Ecuador
    PLoS Negl Trop Dis 4:e915. 2010
    ..We evaluated the molecular diversity of Trypanosoma cruzi, the etiological agent of Chagas disease, in southern Ecuador (Loja Province)...
  16. ncbi The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research
    M A Ferguson
    Division of Molecular Parasitology and Biological Chemistry, Department of Biochemistry, The Wellcome Trust Building, University of Dundee, Dundee DD1 5EH, UK
    J Cell Sci 112:2799-809. 1999
    ..Apart from providing stable membrane anchorage, GPI anchors have been implicated in the sequestration of GPI-anchored proteins into specialised membrane microdomains, known as lipid rafts, and in signal transduction events...
  17. pmc Shotgun sequencing analysis of Trypanosoma cruzi I Sylvio X10/1 and comparison with T. cruzi VI CL Brener
    Oscar Franzén
    Science for Life Laboratory, Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
    PLoS Negl Trop Dis 5:e984. 2011
    b>Trypanosoma cruzi is the causative agent of Chagas disease, which affects more than 9 million people in Latin America...
  18. ncbi Frequency of interferon- gamma -producing T cells specific for Trypanosoma cruzi inversely correlates with disease severity in chronic human Chagas disease
    Susana A Laucella
    Instituto Nacional de Parasitologia Dr Mario Fatala Chaben, Buenos Aires, Argentina
    J Infect Dis 189:909-18. 2004
    This study sought to quantify CD8(+) T cell responses to Trypanosoma cruzi and to identify potential links between these responses and the severity of disease in humans...
  19. ncbi Chagas disease in Spain, the United States and other non-endemic countries
    Joaquim Gascon
    Centre de Salut Internacional, CRESIB, Hospital Clinic, IDIBAPS, C Villarroel, 170, 08036, Barcelona, Spain
    Acta Trop 115:22-7. 2010
    ..countries now living in North America, Europe, Australia and Japan, including thousands of people with Trypanosoma cruzi infection. Most infected individuals are not aware of their status...
  20. ncbi The molecular epidemiology and phylogeography of Trypanosoma cruzi and parallel research on Leishmania: looking back and to the future
    M A Miles
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, UK
    Parasitology 136:1509-28. 2009
    b>Trypanosoma cruzi is the protozoan agent of Chagas disease, and the most important parasitic disease in Latin America...
  21. ncbi A new genotype of Trypanosoma cruzi associated with bats evidenced by phylogenetic analyses using SSU rDNA, cytochrome b and Histone H2B genes and genotyping based on ITS1 rDNA
    A Marcili
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo USP, SP, Brasil
    Parasitology 136:641-55. 2009
    We characterized 15 Trypanosoma cruzi isolates from bats captured in the Amazon, Central and Southeast Brazilian regions. Phylogenetic relationships among T...
  22. ncbi A role for extracellular amastigotes in the immunopathology of Chagas disease
    J Scharfstein
    Laboratório de Imunologia Molecular, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil
    Mem Inst Oswaldo Cruz 94:51-63. 1999
    In spite of the growing knowledge obtained about immune control of Trypanosoma cruzi infection, the mechanisms responsible for the variable clinico-pathological expression of Chagas disease remain unknown...
  23. ncbi Chagas cardiomyopathy--where do we stand after a hundred years?
    Andreia Biolo
    Division of Cardiology, Hospital de Clínicas de Porto Alegre and Faculty of Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil
    Prog Cardiovasc Dis 52:300-16. 2010
    ..In this article, we review the main clinical aspects of Chagas cardiomyopathy and underscore some upcoming challenges for the appropriate control, diagnosis, and management of this complex disease...
  24. pmc Analyses of 32 loci clarify phylogenetic relationships among Trypanosoma cruzi lineages and support a single hybridization prior to human contact
    Carlos A Flores-López
    Department of Biology, University of Maryland, College Park, MD, USA
    PLoS Negl Trop Dis 5:e1272. 2011
    The genetic diversity of Trypanosoma cruzi, the etiological agent of Chagas disease, has been traditionally divided in two major groups, T...
  25. ncbi Oxidative damage during chagasic cardiomyopathy development: role of mitochondrial oxidant release and inefficient antioxidant defense
    Jian Jun Wen
    Department of Microbiology and Immunology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA
    Free Radic Biol Med 37:1821-33. 2004
    ..we evaluated the oxidant status and antioxidant defense capabilities of the heart during the course of Trypanosoma cruzi infection and disease development in a murine model system...
  26. ncbi Characterization of a 21kDa protein from Trypanosoma cruzi associated with mammalian cell invasion
    Claudio V da Silva
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil
    Microbes Infect 11:563-70. 2009
    b>Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion...
  27. ncbi Invasion of MDCK epithelial cells with altered expression of Rho GTPases by Trypanosoma cruzi amastigotes and metacyclic trypomastigotes of strains from the two major phylogenetic lineages
    Adriana B Fernandes
    Departamento de Microbiologia, Immunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de Sao Paulo UNIFESP, Rua Botucatu, 862 6th floor, 04023 062, Sao Paulo, SP, Brazil
    Microbes Infect 6:460-7. 2004
    In order to invade mammalian cells, Trypanosoma cruzi infective forms cause distinct rearrangements of membrane and host cell cytoskeletal components...
  28. ncbi Roles of naturally occurring protease inhibitors in the modulation of host cell signaling and cellular invasion by Trypanosoma cruzi
    Julio Scharfstein
    Lnstituto de Biofisica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil
    Subcell Biochem 47:140-54. 2008
    b>Trypanosoma cruzi trypomastigotes rely on the structural diversity of the cruzipain family of cysteine proteases to infect and multiply in nonprofessional phagocytic cells...
  29. pmc Recent, independent and anthropogenic origins of Trypanosoma cruzi hybrids
    Michael D Lewis
    Department of Pathogen Molecular Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    PLoS Negl Trop Dis 5:e1363. 2011
    The single celled eukaryote Trypanosoma cruzi, a parasite transmitted by numerous species of triatomine bug in the Americas, causes Chagas disease in humans. T...
  30. ncbi Starvation and rapamycin differentially regulate host cell lysosome exocytosis and invasion by Trypanosoma cruzi metacyclic forms
    Rafael Miyazawa Martins
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Rua Botucatu 862, Sao Paulo, Brazil
    Cell Microbiol 13:943-54. 2011
    ....
  31. ncbi Invasion mechanisms among emerging food-borne protozoan parasites
    Nobuko Yoshida
    Department of Microbiology, Immunology and Parasitology, Universidade Federal de Sao Paulo, R Pedro de Toledo 669, Sao Paulo, Brasil
    Trends Parasitol 27:459-66. 2011
    ..Among the emerging protozoan parasites, the most prominent is Trypanosoma cruzi, rarely reported in the past to be transmitted by the oral route but currently responsible for frequent ..
  32. ncbi Ergosterol biosynthesis and drug development for Chagas disease
    Julio A Urbina
    Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela
    Mem Inst Oswaldo Cruz 104:311-8. 2009
    ..presents an overview of the currently available drugs nifurtimox (NFX) and benznidazole (BZN) used against Trypanosoma cruzi, the aetiological agent of Chagas disease; herein we discuss their limitations along with potential ..
  33. ncbi The effects on Trypanosoma cruzi of novel synthetic naphthoquinones are mediated by mitochondrial dysfunction
    Rubem F S Menna-Barreto
    Laboratorio de Biologia Celular, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Rio de Janeiro, RJ 21040 900, Brazil
    Free Radic Biol Med 47:644-53. 2009
    ..isolated from Brazilian flora, and their derivatives, as alternative chemotherapeutic agents against Trypanosoma cruzi. From C-allyl lawsone three naphthofuranquinones were synthesized, which were active against trypomastigotes ..
  34. ncbi Predominance of lineage I among Trypanosoma cruzi isolates from Venezuelan patients with different clinical profiles of acute Chagas' disease
    Néstor Añez
    Departamento de Biologia, Universidad de Los Andes, Facultad de Ciencias, Merida, Venezuela
    Trop Med Int Health 9:1319-26. 2004
    b>Trypanosoma cruzi isolates from 23 acute chagasic patients from localities of Western Venezuela (state of Barinas) where Chagas' disease is endemic were typed using ribosomal and mini-exon gene markers...
  35. ncbi Large urban outbreak of orally acquired acute Chagas disease at a school in Caracas, Venezuela
    Belkisyolé Alarcón de Noya
    Departments of Immunology, Instituto de Medicina Tropical, Universidad Central de Venezuela Código Postal 1041, Los Chaguaramos, Caracas, Venezuela
    J Infect Dis 201:1308-15. 2010
    b>Trypanosoma cruzi oral transmission is possible through food contamination by vector's feces. Little is known about the epidemiology and clinical features of microepidemics of orally acquired acute Chagas disease (CD).
  36. ncbi Rapid quantitation of Trypanosoma cruzi in host tissue by real-time PCR
    Kara L Cummings
    Department of Cellular Biology, Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
    Mol Biochem Parasitol 129:53-9. 2003
    ..and sensitive quantitation of tissue parasite burden in animals infected with the protozoan parasite Trypanosoma cruzi was developed...
  37. ncbi Trypanosoma cruzi in Brazilian Amazonia: Lineages TCI and TCIIa in wild primates, Rhodnius spp. and in humans with Chagas disease associated with oral transmission
    Arlei Marcili
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, 05508 900 Sao Paulo, SP, Brazil
    Int J Parasitol 39:615-23. 2009
    In this study, we provide phylogenetic and biogeographic evidence that the Trypanosoma cruzi lineages T. cruzi I (TCI) and T...
  38. pmc Host cell invasion by Trypanosoma cruzi is potentiated by activation of bradykinin B(2) receptors
    J Scharfstein
    Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CEP 21990 400 Rio de Janeiro, Brazil
    J Exp Med 192:1289-300. 2000
    The parasitic protozoan Trypanosoma cruzi employs multiple molecular strategies to invade a broad range of nonphagocytic cells...
  39. ncbi Uptake of apoptotic cells drives the growth of a pathogenic trypanosome in macrophages
    C G Freire-de-Lima
    Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, RJ, Brazil
    Nature 403:199-203. 2000
    ..in an experimental model of Chagas' disease, a debilitating cardiac illness caused by the protozoan Trypanosoma cruzi. Here we show that the interaction of apoptotic, but not necrotic T lymphocytes with macrophages infected ..
  40. ncbi Susceptibility and natural resistance of Trypanosoma cruzi strains to drugs used clinically in Chagas disease
    L S Filardi
    Centro de Pesquisas Rene Rachou, FIOCRUZ, Belo Horizonte, Brazil
    Trans R Soc Trop Med Hyg 81:755-9. 1987
    ..derivatives used clinically in Chagas disease (nifurtimox and benznidazole) were investigated in 47 Trypanosoma cruzi strains isolated from human patients, domestic vectors and sylvatic reservoirs or vectors...
  41. ncbi The endless race between Trypanosoma cruzi and host immunity: lessons for and beyond Chagas disease
    Caroline Junqueira
    Laboratorio de Immunopatologia, Centro de Pesquisas Rene Rachou, Fundacao Oswaldo Cruz, Belo Horizonte, MG, Brazil
    Expert Rev Mol Med 12:e29. 2010
    Infection with the protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, is characterised by a variable clinical course - from symptomless cases to severe chronic disease with cardiac and/or gastrointestinal involvement...
  42. ncbi An estimate of the burden of Chagas disease in the United States
    Caryn Bern
    Division of Parasitic Diseases, National Center for Zoonotic, Vector Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Clin Infect Dis 49:e52-4. 2009
    ..published seroprevalence figures to immigrant populations, we estimate that 300,167 individuals with Trypanosoma cruzi infection live in the United States, with 30,000-45,000 cardiomyopathy cases and 63-315 congenital ..
  43. ncbi Mechanism of genetic exchange in American trypanosomes
    Michael W Gaunt
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Nature 421:936-9. 2003
    The kinetoplastid Protozoa are responsible for devastating diseases. In the Americas, Trypanosoma cruzi is the agent of Chagas' disease--a widespread disease transmissible from animals to humans (zoonosis)--which is transmitted by ..
  44. pmc Accurate real-time PCR strategy for monitoring bloodstream parasitic loads in chagas disease patients
    Tomas Duffy
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    PLoS Negl Trop Dis 3:e419. 2009
    This report describes a real-time PCR (Q-PCR) strategy to quantify Trypanosoma cruzi (T. cruzi) DNA in peripheral blood samples from Chagas disease patients targeted to conserved motifs within the repetitive satellite sequence.
  45. pmc Perforin and gamma interferon expression are required for CD4+ and CD8+ T-cell-dependent protective immunity against a human parasite, Trypanosoma cruzi, elicited by heterologous plasmid DNA prime-recombinant adenovirus 5 boost vaccination
    Bruna C G de Alencar
    Centro Interdisciplinar de Terapia Gênica CINTERGEN, Universidade Federal de Sao Paulo Escola Paulista de Medicina, Rua Mirassol 207, Sao Paulo, SP 04044 010, Brazil
    Infect Immun 77:4383-95. 2009
    ..immunity (reduction of acute parasitemia and prolonged survival) against experimental infection with Trypanosoma cruzi. Protective immunity correlated with the presence of in vivo antigen-specific cytotoxic activity prior to ..
  46. ncbi Chemokines, inflammation and Trypanosoma cruzi infection
    Mauro M Teixeira
    Departmento Bioquímica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, Pampulha, 31270 901, Belo Horizonte, MG, Brazil
    Trends Parasitol 18:262-5. 2002
    The inflammatory response that follows the infection with Trypanosoma cruzi is essential for host resistance to infection but is also responsible for the diverse pathology observed in Chagas disease...
  47. pmc CD8+ T cells in Trypanosoma cruzi infection
    Angel M Padilla
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
    Curr Opin Immunol 21:385-90. 2009
    CD8(+) T cells have emerged as crucial players in the control of a number of protozoan pathogens, including Trypanosoma cruzi, the agent of human Chagas disease. The recent identification of the dominant targets of T...
  48. pmc Cysteine protease inhibitors cure an experimental Trypanosoma cruzi infection
    J C Engel
    Department of Pathology, University of California San Francisco, California 94143, USA
    J Exp Med 188:725-34. 1998
    b>Trypanosoma cruzi is the causative agent of Chagas' disease. The major protease, cruzain, is a target for the development of new chemotherapy...
  49. ncbi The mucin-like glycoprotein super-family of Trypanosoma cruzi: structure and biological roles
    A Acosta-Serrano
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biochem Parasitol 114:143-50. 2001
    b>Trypanosoma cruzi expresses at its surface large amounts of mucin-like glycoproteins. The T...
  50. ncbi The development of Trypanosoma cruzi in triatominae
    A H Kollien
    Department of Special Zoology, Ruhr University Bochum, D 44780 Bochum, Germany
    Parasitol Today 16:381-7. 2000
    b>Trypanosoma cruzi multiplies and differentiates in the digestive tract of triatomine insects...
  51. ncbi Development of a real-time PCR assay for Trypanosoma cruzi detection in blood samples
    Maria Piron
    Transfusion Safety Laboratory, Banc de Sang i Teixits, Barcelona, Spain
    Acta Trop 103:195-200. 2007
    The aim of this study was to develop a real-time PCR technique to detect Trypanosoma cruzi DNA in blood of chagasic patients. Analytical sensitivity of the real-time PCR was assessed by two-fold serial dilutions of T...
  52. pmc Genome-scale multilocus microsatellite typing of Trypanosoma cruzi discrete typing unit I reveals phylogeographic structure and specific genotypes linked to human infection
    Martin S Llewellyn
    London School of Hygiene and Tropical Medicine, London, UK
    PLoS Pathog 5:e1000410. 2009
    b>Trypanosoma cruzi is the most important parasitic infection in Latin America and is also genetically highly diverse, with at least six discrete typing units (DTUs) reported: Tc I, IIa, IIb, IIc, IId, and IIe...
  53. pmc Ancestral genomes, sex, and the population structure of Trypanosoma cruzi
    Jorge M de Freitas
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
    PLoS Pathog 2:e24. 2006
    Acquisition of detailed knowledge of the structure and evolution of Trypanosoma cruzi populations is essential for control of Chagas disease...
  54. pmc Widespread, focal copy number variations (CNV) and whole chromosome aneuploidies in Trypanosoma cruzi strains revealed by array comparative genomic hybridization
    Todd A Minning
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA
    BMC Genomics 12:139. 2011
    b>Trypanosoma cruzi is a protozoan parasite and the etiologic agent of Chagas disease, an important public health problem in Latin America. T...
  55. pmc Regulatory T cells phenotype in different clinical forms of Chagas' disease
    Fernanda Fortes de Araújo
    Laboratorio de Imunologia Celular e Molecular, Centro de Pesquisas Rene Rachou, FIOCRUZ, Belo Horizonte, Brasil
    PLoS Negl Trop Dis 5:e992. 2011
    ..Ex vivo immunophenotyping of whole blood, as well as after stimulation with Trypanosoma cruzi antigens, demonstrated that individuals in the indeterminate (IND) clinical form of the disease have a ..
  56. ncbi The role of parasite persistence in pathogenesis of Chagas heart disease
    F R S Gutierrez
    Department of Biochemistry and Immunology, Ribeirao Preto School of Medicine, University of Sao Paulo, Sao Paulo, Brazil
    Parasite Immunol 31:673-85. 2009
    Chagas disease (CD) is caused by the infection with the protozoan haemoflagellate Trypanosoma cruzi. This disease is still a great menace to public health, and is largely neglected as it affects mostly the poorest populations of Latin ..
  57. pmc Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in Trypanosoma cruzi
    Matthew Yeo
    London School of Hygiene and Tropical Medicine, London, United Kingdom
    PLoS Negl Trop Dis 5:e1049. 2011
    ..b>Trypanosoma cruzi, the protozoan agent of American trypanosomiasis (Chagas disease), has remarkable genetic and ecological ..
  58. pmc Trypanosoma cruzi induces the reactive oxygen species-PARP-1-RelA pathway for up-regulation of cytokine expression in cardiomyocytes
    Xueqing Ba
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 285:11596-606. 2010
    ..human cardiomyocytes (AC16) produce reactive oxygen species (ROS) and inflammatory cytokines in response to Trypanosoma cruzi. ROS were primarily produced by mitochondria, some of which diffused to cytosol of infected cardiomyocytes...
  59. pmc Genome size, karyotype polymorphism and chromosomal evolution in Trypanosoma cruzi
    Renata T Souza
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil
    PLoS ONE 6:e23042. 2011
    The Trypanosoma cruzi genome was sequenced from a hybrid strain (CL Brener). However, high allelic variation and the repetitive nature of the genome have prevented the complete linear sequence of chromosomes being determined...
  60. ncbi Trypanosoma cruzi infection: a continuous invader-host cell cross talk with participation of extracellular matrix and adhesion and chemoattractant molecules
    A P M P Marino
    Laboratório de Autoimunidade e Imuno regulação, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brasil
    Braz J Med Biol Res 36:1121-33. 2003
    Several lines of evidence have shown that Trypanosoma cruzi interacts with host extracellular matrix (ECM) components producing breakdown products that play an important role in parasite mobilization and infectivity...
  61. ncbi Host cell invasion mediated by Trypanosoma cruzi surface molecule gp82 is associated with F-actin disassembly and is inhibited by enteroinvasive Escherichia coli
    Mauro Cortez
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, R Botucatu, 862, 6th andar, 04023 062 Sao Paulo, Brazil
    Microbes Infect 8:1502-12. 2006
    The target cell F-actin disassembly, induced by a Ca2+-signaling Trypanosoma cruzi factor of unknown molecular identity, has been reported to promote parasite invasion...
  62. ncbi The peptidases of Trypanosoma cruzi: digestive enzymes, virulence factors, and mediators of autophagy and programmed cell death
    Vanina E Alvarez
    Instituto de Investigaciones Biotecnologicas IIB INTECH, Universidad Nacional de San Martín CONICET, Buenos Aires, Argentina
    Biochim Biophys Acta 1824:195-206. 2012
    b>Trypanosoma cruzi, the agent of the American Trypanosomiasis, Chagas disease, contains cysteine, serine, threonine, aspartyl and metallo peptidases...
  63. ncbi Novel strategy in Trypanosoma cruzi cell invasion: implication of cholesterol and host cell microdomains
    Maria Cecilia Fernandes
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Rua Botucatu, Sao Paulo, SP, Brazil
    Int J Parasitol 37:1431-41. 2007
    b>Trypanosoma cruzi, the etiological agent of Chagas' disease, is an obligatory intracellular parasite in the mammalian host. In order to invade a wide variety of mammalian cells, T...
  64. ncbi Cooperative activation of TLR2 and bradykinin B2 receptor is required for induction of type 1 immunity in a mouse model of subcutaneous infection by Trypanosoma cruzi
    Ana Carolina Monteiro
    Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro UFRJ, Centre de Ciências da Saúde, Sala D 007, Rio de Janeiro, Brazil
    J Immunol 177:6325-35. 2006
    ..In this study, we show that these premises are met in a model of s.c. infection by Trypanosoma cruzi, a protozoan that liberates kinins from kininogens through its major protease, cruzipain...
  65. pmc Subdominant/cryptic CD8 T cell epitopes contribute to resistance against experimental infection with a human protozoan parasite
    Mariana R Dominguez
    Centro de Terapia Celular e Molecular CTCMol, Universidade Federal de Sao Paulo Escola Paulista de Medicina, Sao Paulo, Brazil
    PLoS ONE 6:e22011. 2011
    ..Earlier, we have shown that during experimental infection, the human intracellular pathogen Trypanosoma cruzi restricts the repertoire of CD8(+) T cells generating strong immunodominance...
  66. ncbi The evolution of Trypanosoma cruzi: the 'bat seeding' hypothesis
    Patrick B Hamilton
    Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, United Kingdom
    Trends Parasitol 28:136-41. 2012
    Recent discussions on the evolution of Trypanosoma cruzi have been dominated by the southern super-continent hypothesis, whereby T...
  67. ncbi Mammalian cell invasion and intracellular trafficking by Trypanosoma cruzi infective forms
    Renato A Mortara
    Departamento de Microbiologia, Imunologia e Parasitologia, UNIFESP, Escola Paulista de Medicina, Rua Botucatu, 862, 6 andar, 04023 062 Sao Paulo, SP, Brazil
    An Acad Bras Cienc 77:77-94. 2005
    b>Trypanosoma cruzi, the etiological agent of Chagas disease, occurs as different strains or isolates that may be grouped in two major phylogenetic lineages: T. cruzi I, associated with the sylvatic cycle and T...
  68. ncbi The challenge of Chagas' disease: has the human pathogen, Trypanosoma cruzi, learned how to modulate signaling events to subvert host cells?
    F M Lima
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Sao Paulo, SP, Brazil
    N Biotechnol 27:837-43. 2010
    Chagas' disease, caused by Trypanosoma cruzi, is an urgent and highly prevalent danger that is endemic to Latin America, and which the research community continues to ignore...
  69. pmc Variability of kinetoplast DNA gene signatures of Trypanosoma cruzi II strains from patients with different clinical forms of Chagas' disease in Brazil
    Eliane Lages-Silva
    Departamento de Ciencias Biologicas, Universidade Federal do Triangulo Mineiro, Uberaba, Brazil
    J Clin Microbiol 44:2167-71. 2006
    ..Our research aims at characterizing the genetic profiles of Trypanosoma cruzi isolates recently obtained from 70 chagasic patients who either showed pathological lesions with symptoms of ..
  70. ncbi IL-17 is necessary for host protection against acute-phase Trypanosoma cruzi infection
    Yoshiyuki Miyazaki
    Division of Molecular and Cellular Immunoscience, Department of Biomolecular Sciences, Saga University, Saga, Japan
    J Immunol 185:1150-7. 2010
    ..Therefore, we experimentally infected IL-17A-deficient (IL-17A(-/-)) mice with Trypanosoma cruzi. IL-17A(-/-) mice had a lower survival rate and prolonged worse parasitemia compared with control C57BL/6 ..
  71. ncbi Involvement of Ssp-4-related carbohydrate epitopes in mammalian cell invasion by Trypanosoma cruzi amastigotes
    Claudio Vieira da Silva
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Rua Botucatu, 862, 60 Andar, São Paulo 04023 062, Brazil
    Microbes Infect 8:2120-9. 2006
    ..carbohydrate epitopes defined by monoclonal antibodies 1D9 and 2B7 was related to cell invasion by Trypanosoma cruzi amastigotes from different isolates and whether the highest expression of the epitope defined by MAb 1D9 ..
  72. pmc Extraordinary Trypanosoma cruzi diversity within single mammalian reservoir hosts implies a mechanism of diversifying selection
    Martin S Llewellyn
    The London School of Hygiene and Tropical Medicine, London, UK
    Int J Parasitol 41:609-14. 2011
    b>Trypanosoma cruzi is an evolutionarily ancient parasitic protozoan endemic to the Americas...
  73. ncbi Further interest of miniexon multiplex PCR for a rapid typing of Trypanosoma cruzi DTU groups
    Claudia Aliaga
    Institut de Recherche pour le Developpement, Society and Health Department, MIVEGEC, University of Montpellier CNRS 5290 IRD 224, Representation in Bolivia, Av Hernando Siles N 5290, CP 9214, La Paz, Bolivia
    Infect Genet Evol 11:1155-8. 2011
    In order to validate a rapid typing of Trypanosoma cruzi DTUs, the miniexon multiplex PCR was tested for the first time, on a large and diversified sample of 70 strains belonging to all current DTUs (TcI to TcVI)...
  74. pmc Inducible nitric oxide synthase is not essential for control of Trypanosoma cruzi infection in mice
    Kara L Cummings
    Center for Tropical and Emerging Global Diseases, 623 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA
    Infect Immun 72:4081-9. 2004
    Immune control of many intracellular pathogens, including Trypanosoma cruzi, is reported to be dependent on the production of nitric oxide...
  75. pmc Silencing of the laminin gamma-1 gene blocks Trypanosoma cruzi infection
    Pius N Nde
    Division of Microbial Pathogenesis and Immune Response, Department of Biomedical Sciences, School of Medicine, Meharry Medical College, 1005 Dr D B Todd Jr Blvd, Nashville, TN 37208, USA
    Infect Immun 74:1643-8. 2006
    It is thought that Trypanosoma cruzi, the protozoan that causes Chagas' disease, modulates the extracellular matrix network to facilitate infection of human cells. However, direct evidence to document this phenomenon is lacking...
  76. pmc The CC chemokine receptor 5 is important in control of parasite replication and acute cardiac inflammation following infection with Trypanosoma cruzi
    Jenny L Hardison
    Department of Molecular Biology and Biochemistry, 3205 McGaugh Hall, University of California Irvine, Irvine, CA 92697 3900, USA
    Infect Immun 74:135-43. 2006
    Infection of susceptible mice with the Colombiana strain of Trypanosoma cruzi results in an orchestrated expression of chemokines and chemokine receptors within the heart that coincides with parasite burden and cellular infiltration...
  77. pmc Profiling the Trypanosoma cruzi phosphoproteome
    Fabricio K Marchini
    Instituto Carlos Chagas, FIOCRUZ, Curitiba, Parana, Brazil
    PLoS ONE 6:e25381. 2011
    ..To in depth characterize the phosphoproteome of Trypanosoma cruzi, a parasite of the Kinetoplastida class, protein samples from cells at different phases of the ..
  78. pmc Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi
    Paula Ordonhez Rigato
    CTCMol, UNIFESP, Escola Paulista de Medicina, Rua Mirassol 207, Sao Paulo, SP, Brazil 04044 010
    Infect Immun 79:2120-30. 2011
    ..immunity against experimental systemic infection of mice with a human intracellular protozoan parasite, Trypanosoma cruzi. In the present study, we further characterized the protective long-lived CD8(+) T cells...
  79. ncbi Fighting the oxidative assault: the Trypanosoma cruzi journey to infection
    Lucia Piacenza
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo Uruguay, Uruguay
    Curr Opin Microbiol 12:415-21. 2009
    ..the concomitant generation of oxidant species is a medullar innate immune process for the control of acute Trypanosoma cruzi infection...
  80. ncbi The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease
    Najib M El-Sayed
    Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
    Science 309:409-15. 2005
    Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that the diploid genome contains a predicted 22,570 proteins encoded by genes, of which 12,570 represent allelic pairs...
  81. pmc CD8+-T-cell-dependent control of Trypanosoma cruzi infection in a highly susceptible mouse strain after immunization with recombinant proteins based on amastigote surface protein 2
    Adriano F S Araújo
    CINTERGEN, UNIFESP Escola Paulista de Medicina, Rua Mirassol, 207, São Paulo SP 04044 010, Brazil
    Infect Immun 73:6017-25. 2005
    ..surface protein 2 (ASP-2) protects approximately 65% of highly susceptible A/Sn mice against the lethal Trypanosoma cruzi infection...
  82. pmc Genetic transformation of a Corynebacterial symbiont from the Chagas disease vector Triatoma infestans
    Ravi V Durvasula
    Department of Internal Medicine, University of New Mexico, 915 Camino Del Salud NE, CRF 305, Albuquerque, NM 87131, USA
    Exp Parasitol 119:94-8. 2008
    ..We have modified this bacterium to produce an immunologically active single chain antibody fragment, termed rDB3. This study establishes the basis for generating paratransgenic T. infestans as a strategy for control of Chagas disease...
  83. pmc A key role for old yellow enzyme in the metabolism of drugs by Trypanosoma cruzi
    Bruno Kilunga Kubata
    Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Osaka 565 0874, Japan
    J Exp Med 196:1241-51. 2002
    b>Trypanosoma cruzi is the etiological agent of Chagas' disease...
  84. ncbi Identification of six Trypanosoma cruzi lineages by sequence-characterised amplified region markers
    S Brisse
    Centre d Etudes sur le Polymorphisme des Microorganismes, UMR CNRS IRD 9926, IRD, 911 Avenue Agropolis, BP 5045, 34 032 Montpellier Cedex, France
    Mol Biochem Parasitol 111:95-105. 2000
    Six discrete phylogenetic lineages were recently identified in Trypanosoma cruzi, on the basis of multilocus enzyme electrophoresis and random amplified polymorphic DNA (RAPD) characterisation...
  85. pmc Inheritance of DNA transferred from American trypanosomes to human hosts
    Mariana M Hecht
    Chagas Disease Multidisciplinary Research Laboratory, Faculty of Medicine, University of Brasilia, Brasilia, Federal District, Brazil
    PLoS ONE 5:e9181. 2010
    ..Instances of naturally occurring human infections by Trypanosoma cruzi are documented, where mitochondrial minicircles integrated mainly into retrotransposable LINE-1 of various ..
  86. pmc Image-based high-throughput drug screening targeting the intracellular stage of Trypanosoma cruzi, the agent of Chagas' disease
    Juan C Engel
    Sandler Center for Basic Research in Parasitic Diseases and Department of Pathology, University of California, San Francisco, UCSF MC 2550, Room 501E, 1700 4th St, San Francisco, CA 94158 2330, USA
    Antimicrob Agents Chemother 54:3326-34. 2010
    Chagas' disease, caused by infection with the parasite Trypanosoma cruzi, is the major cause of heart failure in Latin America...
  87. ncbi Long-term cardiac outcomes of treating chronic Chagas disease with benznidazole versus no treatment: a nonrandomized trial
    Rodolfo Viotti
    Hospital Interzonal General de Agudos Eva Perón and Instituto Nacional de Parasitología Dr Mario Fatala Chaben, Buenos Aires, Argentina
    Ann Intern Med 144:724-34. 2006
    ..Benznidazole is effective for treating acute-stage Chagas disease, but its effectiveness for treating indeterminate and chronic stages remains uncertain...
  88. pmc Perspectives on Trypanosoma cruzi-induced heart disease (Chagas disease)
    Herbert B Tanowitz
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Prog Cardiovasc Dis 51:524-39. 2009
    Chagas disease is caused by the parasite Trypanosoma cruzi. It is a common cause of heart disease in endemic areas of Latin America...
  89. pmc Drug-induced cure drives conversion to a stable and protective CD8+ T central memory response in chronic Chagas disease
    Juan M Bustamante
    Center for Tropical and Emerging Global Diseases, Coverdell Center, 500 D W Brooks Drive, University of Georgia, Athens, Georgia 30602, USA
    Nat Med 14:542-50. 2008
    ..By establishing a system for drug cure of chronic Trypanosoma cruzi infection, we present the first extensively documented case of total parasite clearance after drug treatment ..
  90. pmc A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes
    Shane R Wilkinson
    School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, United Kingdom
    Proc Natl Acad Sci U S A 105:5022-7. 2008
    ..Loss of a single copy of this gene in Trypanosoma cruzi, either through in vitro drug selection or by targeted gene deletion, is sufficient to cause significant ..
  91. pmc Chagas heart disease pathogenesis: one mechanism or many?
    Kevin M Bonney
    Department of Pathology and Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    Curr Mol Med 8:510-8. 2008
    Chagas heart disease (CHD), caused by the protozoan parasite Trypanosoma cruzi, is the leading cause of infectious myocarditis in the world...
  92. ncbi Evidence for genetic exchange and hybridization in Trypanosoma cruzi based on nucleotide sequences and molecular karyotype
    Sylvain Brisse
    Génétique des Maladies Infectieuses, UMR CNRS IRD No 9926, Institut de Recherches pour le Dévelopement, Montpellier, France
    Infect Genet Evol 2:173-83. 2003
    b>Trypanosoma cruzi is thought to undergo predominant clonal evolution, as determined by population genetics studies...
  93. pmc Cardiac myosin autoimmunity in acute Chagas' heart disease
    J S Leon
    Department of Pathology and Feinberg Cardiovascular Research Institute, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Infect Immun 69:5643-9. 2001
    Infection with Trypanosoma cruzi, the agent of Chagas' disease, may induce antibodies and T cells reactive with self antigens (autoimmunity)...
  94. ncbi Proteomic analysis of Trypanosoma cruzi resistance to Benznidazole
    Hélida M Andrade
    Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisa Rene Rachou FIOCRUZ, Brazil
    J Proteome Res 7:2357-67. 2008
    The first proteomic analysis of Trypanosoma cruzi resistance to Benznidazole (BZ) is presented. The differential proteome of T...
  95. ncbi Central role of extracellular signal-regulated kinase and Toll-like receptor 4 in IL-10 production in regulatory dendritic cells induced by Trypanosoma cruzi
    Carolina V Poncini
    Departamento de Microbiologia, Parasitologia e Inmunologia, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 13, 1121 Ciudad Autónoma de Buenos Aires, Argentina
    Mol Immunol 47:1981-8. 2010
    Several Trypanosoma cruzi molecules that stimulate macrophages activity were described as Toll-like receptor 2 (TLR2) ligands. Besides, the models of dendritic cells (DC) are poorly characterised...
  96. ncbi The ubiquitin-proteasome pathway plays an essential role in proteolysis during Trypanosoma cruzi remodeling
    J L de Diego
    Michael Heidelberger Division, Pathology Department, New York University School of Medicine, New York, New York 10016, USA
    Biochemistry 40:1053-62. 2001
    ..The 26S proteasome of Trypanosoma cruzi epimastigotes was identified as a high molecular weight complex (1400 kDa) with an ATP-dependent ..
  97. ncbi Reactivation of Chagas disease with central nervous system involvement in HIV-infected patients in Argentina, 1992-2007
    Ezequiel Cordova
    Francisco J Muñiz Infectious Diseases Hospital, Uspallata 2262, Buenos Aires, Argentina
    Int J Infect Dis 12:587-92. 2008
    ..The objective of this study was to evaluate clinical and microbiological characteristics of Chagas disease (ChD) with central nervous system (CNS) involvement in AIDS patients...
  98. ncbi Mode of action of nifurtimox and N-oxide-containing heterocycles against Trypanosoma cruzi: is oxidative stress involved?
    Mariana Boiani
    Laboratorio de Química Orgánica, Facultad de Ciencias Facultad de Química, Universidad de la Republica, Montevideo, Uruguay
    Biochem Pharmacol 79:1736-45. 2010
    Chagas disease is caused by the trypanosomatid parasite Trypanosoma cruzi and threatens millions of lives in South America...
  99. ncbi Characterization of isolated acidocalcisomes of Trypanosoma cruzi
    D A Scott
    Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 275:24215-21. 2000
    ..we describe a new method using iodixanol density gradients for purification of the acidocalcisome from Trypanosoma cruzi epimastigotes...
  100. ncbi Phospholipase A1 from Trypanosoma cruzi infective stages generates lipid messengers that activate host cell protein kinase c
    M L Belaunzarán
    Department of Microbiology, School of Medicine, University of Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina
    Parasitology 134:491-502. 2007
    Here we have studied phospholipase A1 (Plase A1) from Trypanosoma cruzi infective stages and it's possible role regarding the interaction with mammalian host cells...
  101. ncbi Activity of "reversed" diamidines against Trypanosoma cruzi "in vitro"
    C F Silva
    Lab Biologia Celular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil
    Biochem Pharmacol 73:1939-46. 2007
    Chagas' disease is an important parasitic illness caused by the flagellated protozoan Trypanosoma cruzi. The disease affects nearly 17 million individuals in endemic areas of Latin America and the current chemotherapy is quite ..

Research Grants68

  1. Pathogenesis of Oxidative Stress in Chagas Disease
    Nisha Jain Garg; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Chagas disease is caused by the protozoan Trypanosoma cruzi, and represents the third greatest tropical disease burden globally...
  2. Role of Lipoproteins in Chagasic heart disease
    JYOTHI FALGUNI NAGAJYOTHI; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Chagas disease, caused by an intracellular parasite Trypanosoma cruzi, is a major public health problem with significant social and economic implications in most Latin American countries and among ..
  3. Mitochondrial Biomarkers of Cardiomyopathy and Cure in Chagas Disease
    Nisha Jain Garg; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Chagasic cardiomyopathy (CCM) is caused by the protozoan Trypanosoma cruzi, and represents the third greatest tropical disease burden globally...
  4. Rational Development of Anti-Trypanosoma Cruzi Drugs
    Michael H Gelb; Fiscal Year: 2013
    ..b>Trypanosoma cruzi, the etiologic agent of Chagas disease, is widespread in Latin America and causes cardiomyopathy and ..
  5. International Workshop on Opportunistic Protists (IWOP-12, 13 and 14)
    Louis M Weiss; Fiscal Year: 2012
    ..of other neglected pathogens in AIDS patients including the kinetoplastid flagellates Leishmania and Trypanosoma cruzi. IWOP has evolved into a crucial forum for the dissemination of information on diverse and often neglected ..
  6. K777 for treatment of Chagas Disease: IND-enabling Studies and IND Submission
    Robert Don; Fiscal Year: 2012
    ..cysteine protease inhibitor which irreversibly inhibits cruzain, a key protease required for viability of Trypanosoma cruzi, the parasite responsible for Chagas disease...
  7. Role of mucin-type O-glycosylation in Trypanosoma cruzi biology
    Christopher M West; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Trypanosoma cruzi is a single-cell protozoan parasite that causes Chagas disease, characterized by cardiac or gastrointestinal complications in chronically infected patients...
  8. Olfactory and behavioral responses of kissing bugs (Reduviidae: Triatominae), vec
    John G Hildebrand; Fiscal Year: 2010
    ..as kissing bugs, vinchuca, chipo, barbeiro, are blood-sucking insects, vectors of the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas Disease...
  9. Infectious Disease Genomics and Bioinformatics Training in Brazil
    Jessica C Kissinger; Fiscal Year: 2013
    ..These organisms include: Plasmodium sp., Trypanosoma cruzi. Toxoplasma gondii, Schistosoma mansoni. Anopheles, Blomphalaria and Humans...
  10. Geographically robust rapid assay for T. cruzi infection
    SYAMAL X RAYCHAUDHURI; Fiscal Year: 2010
    ..is to develop a standardized and FDA cleared rapid serodiagnostic and confirmatory test for detection of Trypanosoma cruzi (T. cruzi) infections. T...
  11. Synthesis of immunogenic glycolipopeptides for the development of vaccines for Ch
    Katja Michael; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Trypanosoma cruzi is a protozoan parasite that causes Chagas disease in Central America...
  12. Chagas Disease Confirmatory Assay
    Andrew Levin; Fiscal Year: 2009
    ..DESCRIPTION (provided by applicant): Chagas'Disease is a vector-borne parasitic infection of humans with Trypanosoma cruzi, which has recently been identified as an emerging and significant threat to transfusion safety and the ..
  13. Human serum carbonyl proteome in cardiovascular diseases
    Nisha Jain Garg; Fiscal Year: 2010
    Chagasic cardiomyopathy emerges in 30-40% of the patients infected by Trypanosoma cruzi, and accounts for >50,000 deaths and the loss of 2.74 million disability-adjusted life years per year...
  14. Confirmatory Immunoblot Test for Chagas'Disease
    ANDREW E LEVIN; Fiscal Year: 2013
    ..DESCRIPTION (provided by applicant): Chagas'Disease is a vector-borne parasitic infection of humans with Trypanosoma cruzi, which has recently been identified as an emerging and significant threat to transfusion safety and the ..
  15. Pathogenesis of Trypanosoma cruzi infection
    Roberto Docampo; Fiscal Year: 2013
    ..One of the most interesting characteristics of Trypanosoma cruzi, the etiologic agent of Chagas disease, is its possession of two closely associated organelles, the ..
  16. Characterization of Potassium Channels in the Human Parasite Trypanosoma Cruzi
    Veronica Jimenez; Fiscal Year: 2012
    ..provided by applicant): Chagas disease is a zoonotic tropical pathology, caused by the protozoan parasite Trypanosoma cruzi. Endemic in Latin America, it is one of the leading causes of congestive heart failure in the world...
  17. Transcriptome profiling of Trypanosoma cruzi and its host cell
    Najib M El-Sayed; Fiscal Year: 2012
    ..In the present study, we focus on the kinetoplastid protozoan parasite Trypanosoma cruzi, the causative agent of human Chagas'disease...
  18. Trypanosoma cruzi Antioxidant Systems, Virulence and Parasite Persistence in Chag
    Rafael Radi; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Chagas disease caused by the parasitic protozoan Trypanosoma cruzi remains a major public health concern in Latin America and is now spreading worldwide...
  19. Early Detection of Congenital Chagas Disease
    Robert H Gilman; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Twenty-six percent of new Trypanosoma cruzi infections occur through mother-to-child transmission, and as vector and blood donation control improve, the proportion attributable to congenital ..
  20. Global gene expression analysis of Trypanosoma cruzi under hyperosmotic stress
    Roberto Docampo; Fiscal Year: 2012
    ..Mexico in the North to Argentina and Chile in the South, there are 16 to 19 million people infected with Trypanosoma cruzi, the causative agent of Chagas disease. Estimated yearly incidence amounts to 561,000 cases...
  21. Testing DNA Vaccine Against T. cruzi in Large Animal Model (Dogs)
    Nisha Jain Garg; Fiscal Year: 2010
    ..cruzi. Infection by Trypanosoma cruzi causes Chagasic cardiomyopathy in humans...
  22. Mechanistic and structural studies of eukaryotic UDP-galactopyranose mutases
    Pablo Sobrado; Fiscal Year: 2013
    ..fumigatus and Trypanosoma cruzi as prototypes from fungal and protozoan parasites, respectively...
  23. Characterization of Potassium Channels in the Human Parasite Trypanosoma Cruzi
    Veronica Jimenez; Fiscal Year: 2013
    Abstract Chagas disease is a zoonotic tropical pathology, caused by the protozoan parasite Trypanosoma cruzi. Endemic en Latin America, it is one of the leading causes of congestive heart failure in the world...
  24. The Role of Trypanosome Calcium Signaling in Parasite Virulence and Transmission
    Brian T Emmer; Fiscal Year: 2010
    The long-term objective of this application is to understand the molecular mechanisms by which Trypanosoma cruzi and Trypanosoma brucei are able to survive within the mammalian host and cause such profound disease...
  25. A novel vacuolar compartment in Toxoplasma gondii
    Silvia N Moreno; Fiscal Year: 2010
    ..a property that it shares with the plant cell vacuole and the contractile vacuole and acidocalcisomes of Trypanosoma cruzi. Our hypotheses are that: (1) the T...
  26. HTS assay for the intracellular stages of T. cruzi, the vector of Chagas disease
    Juan Engel; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Trypanosoma cruzi is an obligate intracellular parasite that causes Chagas'disease, a chronic and debilitating infection that affects millions of people...
  27. Structures and selective inhibition of tRNA synthetases from pathogenic protozoa
    WILHELMUS HOL; Fiscal Year: 2009
    ..This project focuses on essential proteins from three major global pathogenic protozoa, Trypanosoma brucei, Trypanosoma cruzi and Leishmania species...
  28. Cell Surface Receptors on T Cells and Macrophages
    CORNELIS P TERHORST; Fiscal Year: 2013
    ..microbicidal innate immune responses of macrophages and monocytes to infections by bacteria and the parasite Trypanosoma cruzi. Surprisingly, the co-stimulatory molecule Slamf1 is also a microbial sensor that enters the phagosome for ..
  29. Selective inhibition of tRNA synthetases from pathogenic protozoa
    WILHELMUS G J HOL; Fiscal Year: 2013
    ..This project focuses on essential proteins from three major global pathogenic protozoa, Trypanosoma brucei, Trypanosoma cruzi and Leishmania species...
  30. Ribosome profiling of Trypanosoma brucei
    Marilyn Parsons; Fiscal Year: 2012
    ..Related trypanosomatid pathogens, including Trypanosoma cruzi (the causative agent of Chagas'disease) and numerous Leishmania species (which cause a diverse spectrum ..
  31. STRUCTURE AND MECHANISM OF PROTEIN PRENYLTRANSFERASES
    Lorena S Beese; Fiscal Year: 2013
    ..analysis of protein prenyltransferases from human pathogens, including Candida albicans, Trypanosoma brucei, Trypanosoma cruzi and Plasmodium...
  32. Validating novel drug targets in Trypanosoma cruzi polyamine biosynthesis
    Michael C Gretes; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Trypanosoma cruzi is the protozoan trypanosomatid parasite that causes Chagas disease, which affects 10-12 million overwhelmingly poor people in 22 Latin American countries and an estimated 300,000 ..
  33. Development of Novel Chagas Vaccines
    Daniel F Hoft; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Trypanosoma cruzi is an important human pathogen responsible for 10-50,000 deaths/year infecting 12-20 million people...
  34. Inhibitors Against Trypanosomatid Pyruvate Kinases
    MALCOLM DOUGLAS WALKINSHAW; Fiscal Year: 2012
    ..is to discover and optimize selective inhibitors of pyruvate kinase (PYK) from two protist parasites: Trypanosoma cruzi causes Chagas'disease throughout the Americas, including Texas and other southern regions of the United ..
  35. New molecular techniques for T. cruzi
    Huan Huang; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Trypanosoma cruzi (T. cruzi) causes Chagas Disease (e.g., American Trypanosomiasis) in humans...
  36. Role of DNA Ligands in Innate Immunity
    Nilay Dey; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Chagas disease (CHD) caused by Trypanosoma cruzi (T. cruzi) is endemic in Latin America and an emerging disease in the US and other developed countries. The overall prevalence of human T...
  37. Algorithms and Software for Difficult Proteomics Problems
    Marshall Bern; Fiscal Year: 2009
    ..bacteria, and will perform deeper and more thorough proteomic analyses of highly variable organisms such as Trypanosoma cruzi. More generally, proteomics laboratories worldwide, working on a wide variety of health related projects, ..
  38. The ecology and epidemiology of Chagas disease in urban and rural communities
    CESAR GABRIEL NAQUIRA; Fiscal Year: 2011
    ..epidemiologic, qualitative and spatial methods in concert with high resolution molecular characterization of Trypanosoma cruzi and Triatoma infestans...
  39. Oxidative Response Networks in Chagasic Cardiomyopathy
    Nisha Jain Garg; Fiscal Year: 2013
    ..b>Trypanosoma cruzi is the etiological agent, however, clinical disease does not correlate with parasite presence, and host ..
  40. Structure and Function of the Trypanosome Flagellar Membrane
    David M Engman; Fiscal Year: 2013
    ..A number of closely related calcium-binding proteins are found in the flagella of the protozoan parasites Trypanosoma cruzi and Trypanosoma brucei: a 24 kDa flagellar calcium binding protein (FCaBP) of T...
  41. Trypanosome transmembrane kinases
    Marilyn Parsons; Fiscal Year: 2013
    ..T. brucei has served as a model system for the study of the related trypanosomatid pathogens Trypanosoma cruzi and Leishmania spp., and the three together infect over 12 million people worldwide...
  42. Lyosome Mediated Cell Invasion by Trypanosoma Cruzi
    NORMA WINDSOR ANDREWS; Fiscal Year: 2012
    ..periods of this grant, we learned fundamental aspects of the mechanism by which the protozoan parasite Trypanosoma cruzi gains access to the intracellular environment. Host cell lysosomes play a key role in this process...
  43. Transmission-blocking live vaccine for Chagas disease
    Rick L Tarleton; Fiscal Year: 2013
    ..In many sites, the transmission of Trypanosoma cruzi to insects - and subsequently to the people - is nearly totally determined by the presence or absence of T...
  44. Sterol Biosynthesis in Trypanosomatid Parasites
    Frederick Buckner; Fiscal Year: 2004
    ..is fatal if untreated and cutaneous leishmaniasis causes serious morbidity, Chagas disease is caused by Trypanosoma cruzi and is endemic in over 20 countries in Latin America...
  45. Structure-Aided Design of CYP51 Inhibitors for Treatment of Parasitic Infections
    Larissa M Podust; Fiscal Year: 2013
    ..body of basic research now makes it possible to design drugs that target the sterol biosynthesis pathway in Trypanosoma cruzi, agent of the neglected tropical disease Chagas Disease...
  46. Regional Training Center for Tropical Disease Research in Quito-Ecuador
    Mario J Grijalva; Fiscal Year: 2013
    ..the development of improved standardized genotyping methods for T. cruzi, and their use for Trypanosoma cruzi strain characterization;2...
  47. Exploiting the cAMP pathway in Chagas Disease Therapy
    Daniel L Altschuler; Fiscal Year: 2013
    ..e. Chagas disease. Chagas disease is a life-threatening illness caused by the protozoan parasite Trypanosoma cruzi. Endemic to poor rural areas of South America, with an infected population estimated in 10 million and an ..
  48. Genetic Epidemiology of Chagas Disease Progression
    SARAH A WILLIAMS-BLANGERO; Fiscal Year: 2012
    Chagas disease affects 17 million people and a further 100 million people are at risk for infection with Trypanosoma cruzi, the parasitic cause of Chagas disease, throughout Latin America...
  49. Lead Identification to Clinical Candidate Selection: Drugs for Chagas Disease
    James H McKerrow; Fiscal Year: 2013
    ..highly prevalent throughout Latin America, is a serious and debilitating infection caused by the parasite Trypanosoma cruzi that results in severe cardiomyopathy or megasyndromes...
  50. A novel preribosomal complex in trypanosomes
    Noreen Williams; Fiscal Year: 2013
    ..HEALTH RELEVANCE: The protozoan parasites, the Kinetoplastids, include the organisms Trypanosoma brucei, Trypanosoma cruzi and Leishmania, which cause serious diseases in much of the world (African sleeping sickness, Chagas'..
  51. Identification and Validation of Targets of Phenotypic High Throughput Screening
    Sean Ekins; Fiscal Year: 2013
    ..Disease Project Summary Nearly 10 million people in Latin America are infected with the eukaryotic parasite Trypanosoma cruzi, the causative agent of Chagas disease...
  52. Mathematical Techniques for Control of Epidemic Trypanosoma cruzi Transmission
    Michael Zachary Levy; Fiscal Year: 2012
    Chagas disease, caused by the unicellular parasite Trypanosoma cruzi, is a preventable disease. Nevertheless more people in the Americas die from Chagas disease than any other parasitic infection...
  53. Molecular Analysis of Trypanosome Infection
    Fernando Villalta; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Trypanosoma cruzi is a blood and tissue parasite that affects millions of individuals causing significant human morbidity and mortality...
  54. Development of T cell memory in human Trypanosoma cruzi infection
    Susana Laucella; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Development of T cell memory in human Trypanosoma cruzi infection Trypanosoma cruzi-infected subjects with long-term (>20 years) infections have relatively modest CD4+ and CD8+ T cell responses to ..
  55. Growth factor mimicry in Trypanosoma cruzi invasion of the heart
    MERCIO A PEREIRAPERRIN; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Chagas'disease, caused by the obligate intracellular protozoan Trypanosoma cruzi, is the most important parasitic disease in Latin America, afflicting more than 8 million people, 30-40% of whom ..
  56. Role of host cell metabolism in supporting intracellular Trypanosma cruzi growth
    Barbara A Burleigh; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Trypanosoma cruzi, the protozoan parasite that causes human Chagas'disease, is an obligate intracellular pathogen that lives and divides in the cytoplasm of its mammalian host cell...
  57. ANALYSIS OF THE TRYPANOSOMA BRUCEI GENOME USING TILING ARRAYS
    Christian Tschudi; Fiscal Year: 2009
    ..The completion of the genomes of three trypanosomatids, namely Trypanosoma brucei, Trypanosoma cruzi and Leishmania major, has opened insights into the coding potential and evolution of these genomes...
  58. Disrupting Vector-borne Disease Transmission in Complex Urban Environments
    Michael Zachary Levy; Fiscal Year: 2013
    ..fieldwork in the city of Arequipa, Peru, where transmission of the parasitic agent of Chagas disease, Trypanosoma cruzi, by the insect vector Triatoma infestans is a serious urban problem...
  59. Chemokine-endothelin interaction & Chagas' disease
    Herbert Tanowitz; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant) [unreadable] Chagas' disease, caused by Trypanosoma cruzi, is an important cause of heart disease in Brazil...
  60. MUTATIONAL ANALYSIS OF A PARASITE PURINE SALVAGE ENZYME
    SYDNEY CRAIG; Fiscal Year: 2001
    ..The enzyme that is the subject of this study is the hypoxanthine phosphoribosyltransferase (HPRT) of Trypanosoma cruzi, etiologic agent of Chagas' disease...
  61. TRYPANASOMA CRUZI GENOME-SBRI
    Kenneth Stuart; Fiscal Year: 2004
    We propose to sequence the genome of Trypanosoma cruzi, a human pathogen of global importance, as part of an interactive research project...
  62. ROLE OF CALRETICULIN IN CALCIUM HOMEOSTASIS OF T. CRUZI
    Roberto Docampo; Fiscal Year: 2002
    ..Mexico in the North to Argentina and Chile in the South, there are 18 to 20 million people infected with Trypanosoma cruzi, the causative agent of Chagas' disease...
  63. Development of Dynamic Combinatorial Libraries for Cruzipain Inhibition
    Peter Wipf; Fiscal Year: 2009
    ..for in vitro cruzipain inhibition;(c) compounds having good inhibition activities will be submitted to a Trypanosoma cruzi growth inhibition assay in vitro, in order to evaluate the trypanocidal activity...
  64. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 2002
    ..Mexico in the North to Argentina and Chile in the South, there are 16 to 19 million people infected with Trypanosoma cruzi, the causative agent of Chagas' disease...
  65. RECOMBINANT TRYPANOSOMA CRUZI PROTEIN VACCINES
    LOUIS KIRCHHOFF; Fiscal Year: 1991
    American trypanosomiasis (Chagas' disease) is caused by Trypanosoma cruzi, a protozoan parasite transmitted by reduviid bugs...
  66. ACIDOCALCISOMES IN TRYPANOSOMA CRUZI
    Roberto Docampo; Fiscal Year: 2003
    ..Mexico in the North to Argentina and Chile in the South, there are 16 to 19 million people infected with Trypanosoma cruzi, the causative agent of Chagas' disease...
  67. Trypanosoma cruzi-elicited cardiac hypertrophy
    Christine Petersen; Fiscal Year: 2004
    ..towards heart failure and a frequent consequence of chronic infections with the intracellular pathogen Trypanosoma cruzi, the causative agent of Chagas' disease in humans...
  68. Molecular & Biological Characterization of Trypanosoma cruzi from United States
    Michael Yabsley; Fiscal Year: 2009
    Chagas disease, caused by Trypanosoma cruzi, causes nearly 50,000 deaths a year in Latin America and is a concern in the United States (US) because of the high prevalence of T...