Genomes and Genes
Summary: A genus of protozoa, formerly also considered a fungus. Characteristics include the presence of violet to brown spores.
Publications168 found, 100 shown here
- Effects of Ca2+-ATPase inhibitors, ionomycin, and pharmacological modulators of ryanodine receptor on calcium release from intracellular pools and on oscillatory contractile behavior in Physarum polycephalumA A Kochegarov
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia
Biochemistry (Mosc) 65:662-71. 2000Changes in calcium levels in organelles of the plasmodium of the myxomycete Physarum polycephalum were analyzed using the fluorescent calcium indicator chlortetracycline (CTC)...
- Flow-network adaptation in Physarum amoebaeAtsushi Tero
Research Institute for Electronic Science, Hokkaido University, Sapporo, 060 0812, Japan
Theory Biosci 127:89-94. 2008..The large amoeboid plasmodium of Physarum is able to solve a maze and to connect multiple food locations via a smart network...
- Nucleotide sequence of the Physarum polycephalum small subunit ribosomal RNA as inferred from the gene sequence: secondary structure and evolutionary implicationsT Johansen
Institute of Medical Biology, University of Tromsø, Norway
Curr Genet 14:265-73. 1988The nucleotide sequence of the Physarum polycephalum small subunit ribosomal RNA (SSU rRNA) gene has been determined. Sequence data indicate that the mature 19S SSU rRNA is 1,964 nucleotides long...
- Structure and evolution of myxomycete nuclear group I introns: a model for horizontal transfer by intron homingS Johansen
Institute of Medical Biology, University of Tromsø, Norway
Curr Genet 22:297-304. 1992..positions in the large subunit ribosomal RNA (LSU rRNA) gene of the two myxomycete species, Didymium iridis and Physarum polycephalum. Structural models of intron RNAs, including secondary and tertiary interactions, are proposed...
- Extrachromosomal ribosomal DNA of Didymium iridis: sequence analysis of the large subunit ribosomal RNA gene and sub-telomeric regionS Johansen
Institute of Medical Biology, University of Tromsø, Norway
Curr Genet 22:305-12. 1992..Based on pairwise comparisons of LSU rRNA sequences, the time of divergence between the two myxomycete genera Didymium and Physarum was estimated.
- Selective inhibition of DNA polymerase-alpha family with chemically synthesized derivatives of PHYLPA, a unique Physarum lysophosphatidic acidK Murakami-Murofushi
Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan
Biochim Biophys Acta 1258:57-60. 1995PHYLPA, a unique Physarum lysophosphatidic acid (LPA), showed selective inhibition of a family of DNA polymerase alpha, including DNA polymerases alpha, delta and epsilon; but no inhibition of DNA polymerase beta or gamma was observed...
- Insertional editing of nascent mitochondrial RNAs in PhysarumL M Visomirski-Robic
Department of Molecular Biology and Microbiology, Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
Proc Natl Acad Sci U S A 94:4324-9. 1997Maturation of Physarum mitochondrial RNA involves the highly specific insertion of nonencoded nucleotides at multiple locations...
- Constitutive homologous recombination between mitochondrial DNA and a linear mitochondrial plasmid in Physarum polycephalumH Takano
Department of Biology, Faculty of Science, University of Tokyo, Japan
Curr Genet 22:221-7. 1992In one particular myxamoebal strain (NG7; mF+) of Physarum polycephalum, a linear mitochondrial plasmid (mF plasmid) which promotes mitochondrial fusion has been identified...
- RNA editing in Physarum mitochondria: assays and biochemical approachesElaine M Byrne
Trinity Centre for Engineering, Trinity College Dublin, Dublin, Ireland
Methods Enzymol 424:143-72. 2007Mitochondrial RNAs in the myxomycete Physarum polycephalum differ from the templates from which they are transcribed in defined ways. Most transcripts contain nucleotides that are not present in their respective genes...
- Identification of proteins immunologically related to vertebrate lamins in the nuclear matrix of the myxomycete Physarum polycephalumS Lang
Department of Microbiology, University of Innsbruck Medical School, Austria
Eur J Cell Biol 61:177-83. 1993Lamin-like proteins have been identified as components of the nuclear matrix of the myxomycete Physarum polycephalum...
- Specificity and direction of depolymerization of beta-poly(L-malate) catalysed by polymalatase from Physarum polycephalum--fluorescence labeling at the carboxy-terminus of beta-poly(L-malate)B Gasslmaier
Institut für Biophysik und physikalische Biochemie der Universität Regensburg, Germany
Eur J Biochem 250:308-14. 1997Beta-poly(L-malate), a major constituent of nuclei in plasmodia of Physarum polycephalum, is enzymatically degraded to L-malate after secretion into the culture medium...
- Replication timing of 10 developmentally regulated genes in Physarum polycephalumG Pierron
Institut de Recherches Scientifiques sur le Cancer, Villejuif, France
Nucleic Acids Res 17:553-66. 1989..Within one cell type of Physarum - the plasmodium - we defined the temporal order of replication of 10 genes which were known to be variably ..
- Polyphyletic origin of the genus Physarum (Physarales, Myxomycetes) revealed by nuclear rDNA mini-chromosome analysis and group I intron synapomorphySatish C R Nandipati
RNA and Transcriptomics Group, Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, MH building Breivika, N 9037, Tromsø, Norway
BMC Evol Biol 12:166. 2012..Physarales is of particular interest since the two best-studied myxomycete species, Physarum polycephalum and Didymium iridis, belong to this order and are currently subjected to whole genome and ..
- Identification of the telomeric sequence of the acellular slime molds Didymium iridis and Physarum polycephalumJ Forney
Department of Molecular Biology, University of California, Berkeley 94720
Nucleic Acids Res 15:9143-52. 1987We have determined the telomeric DNA sequence of the acellular slime molds Didymium iridis and Physarum polycephalum. In both organisms the telomeres consist of tandem repeats of the hexamer 5'(TTAGGG)3'...
- Structure of the extrachromosomal ribosomal RNA chromatin of Physarum polycephalumR Lucchini
Institut für Zellbiologie ETH Hönggerberg, Zurich, Switzerland
J Mol Biol 196:829-43. 1987Isolated nucleoli from exponentially growing microplasmodia of Physarum polycephalum were digested with micrococcal nuclease or DNAase I, or were photoreacted with trimethyl psoralen...
- Purification of tropomyosin from Saccharomyces cerevisiae and identification of related proteins in Schizosaccharomyces and PhysarumH P Liu
Section of Biochemistry, Cornell University, Ithaca, NY 14853
Proc Natl Acad Sci U S A 86:90-3. 1989..specifically crossreact with heat-stable Mr 33,000 polypeptides in extracts of Schizosaccharomyces pombe and Physarum polycephalum...
- Non-templated addition of nucleotides to the 3' end of nascent RNA during RNA editing in PhysarumY W Cheng
Center for RNA Molecular Biology, Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA
EMBO J 20:1405-14. 2001RNAs in Physarum: mitochondria contain extra nucleotides that are not encoded by the mitochondrial genome, at least in the traditional sense...
- Mitochondrial DNA of Physarum polycephalum: physical mapping, cloning and transcription mappingE P Jones
Cell and Molecular Biology Program, University of Texas, Dallas, Richardson 75083 0688
Curr Genet 17:331-7. 1990Mitochondrial DNA (mtDNA) has been isolated from four strains of Physarum polycephalum and a restriction site map has been determined using nine restriction enzymes...
- RNA editing by cytidine insertion in mitochondria of Physarum polycephalumR Mahendran
Cell and Molecular Biology Program, University of Texas at Dallas, Richardson 75083 0688
Nature 349:434-8. 1991..The mitochondrial RNA apparently encoding the alpha subunit of ATP synthetase in the acellular slime mould, Physarum polycephalum, is edited at 54 sites by cytidine insertion.
- Insertional editing of mitochondrial tRNAs of Physarum polycephalum and Didymium nigripesT Antes
Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75080, USA
Mol Cell Biol 18:7521-7. 1998tRNAs encoded on the mitochondrial DNA of Physarum polycephalum and Didymium nigripes require insertional editing for their maturation...
- Gene families encode the major encystment-specific proteins of Physarum polycephalum plasmodiaF Bernier
Departement de Biochimie, Faculte des sciences et de genie, Universite Laval, Quebec, Canada
Gene 59:265-77. 1987The encystment of Physarum polycephalum plasmodia, also called spherulation, involves the synthesis of many specific mRNAs and proteins...
- Nucleolar introns from Physarum flavicomum contain insertion elements that may explain how mobile group I introns gained their open reading framesA Vader
Department of Cell Biology, University of Tromsø, Norway
Nucleic Acids Res 22:4553-9. 1994Comparison of two group I intron sequences in the nucleolar genome of the myxomycete Physarum flavicomum to their homologs in the closely related Physarum polycephalum revealed insertion-like elements...
- Involvement of actin dephosphorylation in germination of Physarum sclerotiumKiyoshi Furuhashi
Department of Biology, Faculty of Education, Shiga University, Otsu, Japan
J Eukaryot Microbiol 49:129-33. 2002The plasmodium of Physarum polycephalum grows without cytokinesis and shows an active cytoplasmic streaming under wet and nutritious conditions...
- Purification and partial characterization of transglutaminase from Physarum polycephalumJ D Klein
Graduate Program in Molecular Biology, New Mexico State University, Las Cruces 88003 0001
J Bacteriol 174:2599-605. 1992..818-fold to apparent homogeneity from acetone powder preparations of spherules of the acellular slime mold Physarum polycephalum...
- Methylation is co-ordinated on the putative replication origins of Physarum ribosomal DNAC A Cooney
Department of Biological Chemistry, School of Medicine, University of California, Davis 95616
J Mol Biol 204:889-901. 1988In Physarum polycephalum, the ribosomal DNA is found as 60,000 base-pair palindromes. Each rDNA has four symmetrically arranged replication origins flanked by ribosomal RNA genes...
- Dynamic organization of ATP and birefringent fibrils during free locomotion and galvanotaxis in the plasmodium of Physarum polycephalumT Ueda
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
J Cell Biol 110:1097-102. 1990..of both ATP and birefringent fibrils throughout the cell was studied in the multinuclear ameboid cell of the Physarum plasmodium during free locomotion and galvanotaxis...
- A mobile group I intron in the nuclear rDNA of Physarum polycephalumD E Muscarella
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
Cell 56:443-54. 1989We have shown that a strain-specific group I intron (intron 3) in the nuclear extrachromosomal rDNA or Physarum polycephalum is a mobile element...
- Retrotransposon-like nature of Tp1 elements: implications for the organisation of highly repetitive, hypermethylated DNA in the genome of Physarum polycephalumH M Rothnie
Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, UK
Nucleic Acids Res 19:279-86. 1991The repetitive fraction of the genome of the eukaryotic slime mould Physarum polycephalum is dominated by the Tp1 family of highly repetitive retrotransposon-like sequences...
- Selective gene expression during sporulation of Physarum polycephalumR Martel
Department of Biochemistry, Faculty of Sciences, Laval University, Quebec, Canada
J Bacteriol 170:4784-90. 1988..of polypeptides synthesized in vitro from poly(A)+ RNA showed that mRNA populations change during sporulation of Physarum polycephalum...
- Characterization of I-Ppo, an intron-encoded endonuclease that mediates homing of a group I intron in the ribosomal DNA of Physarum polycephalumD E Muscarella
Section of Biochemistry, Molecular and Cellular Biology, Cornell University, Ithaca, New York 14853
Mol Cell Biol 10:3386-96. 1990..several aspects of I-Ppo, the endonuclease that mediates the mobility of intron 3 in the ribosomal DNA of Physarum polycephalum. This intron is unique among mobile group I introns in that it is located in nuclear DNA...
- Physical relationship between a gene and its origin of replication in Physarum polycephalumM Benard
Laboratoire de Biologie et Ultrastructure du Noyau, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France
Exp Cell Res 186:299-305. 1990Taking advantage of the natural synchrony of the S-phase within the plasmodium of Physarum polycephalum, we extracted highly synchronous DNA samples at precise time points in early S-phase...
- Site-specific initiation of DNA replication within the non-transcribed spacer of Physarum rDNAM Benard
Organisation Fonctionnelle du Noyau, UPR 9044 CNRS, Villejuif, France
Nucleic Acids Res 23:1447-53. 1995b>Physarum polycephalum rRNA genes are found on extrachromosomal 60 kb linear palindromic DNA molecules...
- Changes in diadenosine tetraphosphate levels in Physarum polycephalum with different oxygen concentrationsP N Garrison
Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284 7760
J Bacteriol 171:1506-12. 1989..Ap4A) were measured, by a specific high-pressure liquid chromatography method, in microplasmodia of Physarum polycephalum subjected to different degrees of hypoxia, hyperoxia, and treatment with H2O2...
- Immunoelectron microscopic localization of the nucleolar protein B-36 (fibrillarin) during the cell cycle of Physarum polycephalumG Pierron
Laboratoire de Biologie et Ultrastructure du Noyau, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France
Biol Cell 65:119-26. 1989Monoclonal antibodies raised against the 34-kD nucleolar protein, B-36, from the slime mold Physarum polycephalum have been used to examine the electron microscopic localization of B-36 during the cell cycle in Physarum...
- The F-actin capping proteins of Physarum polycephalum: cap42(a) is very similar, if not identical, to fragmin and is structurally and functionally very homologous to gelsolin; cap42(b) is Physarum actinC Ampe
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium
EMBO J 6:4149-57. 1987We have carried out a primary structure analysis of the F-actin capping proteins of Physarum polycephalum. Cap42(b) was completely sequenced and was found to be identical with Physarum actin...
- Locomotive mechanism of Physarum plasmodia based on spatiotemporal analysis of protoplasmic streamingKenji Matsumoto
Department of Mathematics, Hokkaido University, Sapporo, Japan
Biophys J 94:2492-504. 2008We investigate how an amoeba mechanically moves its own center of gravity using the model organism Physarum plasmodium...
- Actin co-purifies with RNA polymerase IIS S Smith
Biochem Biophys Res Commun 86:161-6. 1979
- Fragmin60 encodes an actin-binding protein with a C2 domain and controls actin Thr-203 phosphorylation in Physarum plasmodia and sclerotiaTatyana Sklyarova
Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Rommelaere Institute, Albert Baertsoenkaai 3, B 9000 Ghent, Belgium
J Biol Chem 277:39840-9. 2002..show that in vivo phosphorylation of actin in the fragmin60-actin complex occurs in sclerotia, a dormant stage of Physarum development, as well as in plasmodia...
- Ca2+-binding modulator protein in protozoa and myxomyceteJ Kuznicki
Cell Biol Int Rep 3:17-23. 1979..with the properties of brain modulator protein of 3,5-cyclic nucleotide phosphodiesterase was identified in Physarum polycephalum plasmodia and in Euglena gracilis and Amoeba proteus cells by urea polyacrylamide gel ..
- Exon junction sequences as cryptic splice sites: implications for intron originTerrie Sadusky
Institute of Reproductive and Developmental Biology, Hammersmith Campus, Imperial College London, London W12 ONN, England
Curr Biol 14:505-9. 2004..identified nine cryptic splice sites within the coding sequence of actin genes from humans, Arabidopsis, and Physarum by inactivating their normal intron splice sites...
- Physarum machines: encapsulating reaction-diffusion to compute spanning treeAndrew Adamatzky
Faculty of Computing, Engineering and Mathematical Sciences, University of the West of England, Bristol, UK
Naturwissenschaften 94:975-80. 2007The Physarum machine is a biological computing device, which employs plasmodium of Physarum polycephalum as an unconventional computing substrate...
- Structural evidence for non-canonical binding of Ca2+ to a canonical EF-hand of a conventional myosinJudit E Debreczeni
Department of Biochemistry, Eotvos Lorand University, Budapest H 1117, Hungary
J Biol Chem 280:41458-64. 2005..previously identified a single inhibitory Ca2+-binding site in the first EF-hand of the essential light chain of Physarum conventional myosin (Farkas, L., Malnasi-Csizmadia, A., Nakamura, A., Kohama, K., and Nyitray, L. (2003) J. Biol...
- Polypropionate lactones of deoxysugars glycosides from slime mold Lycogala epidendrumTomas Rezanka
Institute of Microbiology, Videnska 1083, 14220 Prague, Czech Republic
Phytochemistry 63:945-52. 2003....
- Specific and nonspecific enzymes involved in the catabolism of mononucleoside and dinucleoside polyphosphatesA Guranowski
Katedra Biochemii i Biotechnologii, Akademia Rolnicza, ul Wo gt yOska 35, 60 637, PoznaO, Poland
Pharmacol Ther 87:117-39. 2000..asymmetrically cleaving ones, which are typical of higher eukaryotes, and symmetrically cleaving enzymes found in Physarum polycephalum and in many bacteria...
- Evolution of RNA editing sites in the mitochondrial small subunit rRNA of the MyxomycotaUma Krishnan
Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas, USA
Methods Enzymol 424:197-220. 2007..site location in the core region of the small subunit rRNA gene in the mtDNA of seven myxomycetes, including Physarum polycephalum. We find that the unique type of insertional RNA editing present in mitochondria of P...
- Evolution of hierarchical cytoplasmic inheritance in the plasmodial slime mold Physarum polycephalumAkiko Iwanaga
Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812 8581, Japan
Evolution 58:710-22. 2004..for uniparental mitochondrial transmission is known between many mating types of plasmodial slime mold Physarum polycephalum...
- Structure, function and evolution of the mitochondrial division apparatusTsuneyoshi Kuroiwa
Laboratory of Cell Biology and Frontier Project Life s Adaptation Strategies of Environmental Change, Department of Life Sciences, College of Science, Rikkyo University, 3 34 1 Nishiikebukuro, Toshima ku, Tokyo 171 8501, Japan
Biochim Biophys Acta 1763:510-21. 2006..electron-dense MD ring in the cytoplasm at the constriction sites of dividing mitochondria in the slime mold Physarum polycephalum, and then two large MD rings (with outer cytoplasmic and inner matrix sides) in the red alga ..
- Abundant 5S rRNA-like transcripts encoded by the mitochondrial genome in amoebozoaCharles E Bullerwell
Department of Biochemistry and Molecular Biology, Room 8 F2, Sir Charles Tupper Medical Building, Dalhousie University, 5840 College Street, Halifax, Nova Scotia B3H 1X5, Canada
Eukaryot Cell 9:762-73. 2010..members of Amoebozoa, namely, in the lobose amoeba Hartmannella vermiformis and in the myxomycete slime mold Physarum polycephalum...
- Isolation and characterization of glutathione reductase from Physarum polycephalum and stage-specific expression of the enzyme in life-cycle stages with different oxidation-reduction levelsYoshiko Minami
Department of Biochemistry, Faculty of Science, Okayama University of Science, 1 1 Ridai cho, Okayama 700 0005, Japan
J Eukaryot Microbiol 50:317-23. 2003b>Physarum polycephalum has a life cycle with several distinct phases that have different oxidation-reduction requirements...
- Lens crystallins and their microbial homologs: structure, stability, and functionR Jaenicke
Institut fur Biophysik und Physikalische Biochemie, Universitat Regensburg, Germany
Crit Rev Biochem Mol Biol 36:435-99. 2001..Recent studies of natural and mutant vertebrate bg-crystallins as well as spherulin 3a from Physarum polycephalum and Protein S from Myxococcus xanthus allowed the correlation of structure and stability of ..
- Structure and properties of the recombinant NADH-cytochrome b5 reductase of Physarum polycephalumTerumi Ikegami
Department of Biochemistry, Faculty of Science, Okayama University of Science, Okayama, Japan
Biosci Biotechnol Biochem 71:783-90. 2007A cDNA for NADH-cytochrome b(5) reductase of Physarum polycephalum was cloned from a cDNA library, and the nucleotide sequence of the cDNA was determined (accession no. AB259870)...
- RNA editing is absent in a single mitochondrial gene of Didymium iridisPeter G Hendrickson
Children s Memorial Research Center, Immunology Department, 2300 Children s Plaza, Mailstop 212, Chicago, Illinois 60614, USA
Mycologia 102:1288-94. 2010..The D. iridis sequence was compared to the putative nad3 gene in the related myxomycete Physarum polycephalum, which would require editing. Based on this comparison, editing sites could be predicted for the P...
- Sampling gene diversity across the supergroup Amoebozoa: large EST data sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba spRussell F Watkins
Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5
Protist 159:269-81. 2008..including two free-living amoebae (Acanthamoeba castellanii, Hartmannella vermiformis) and three slime molds (Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp...
- [A SC35-like protein is localized in the nucleus of Physarum polycephalum]Bo Hu
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China
Yi Chuan Xue Bao 31:177-82. 2004After being labeled with an anti-SC35 antibody, the specimens of Physarum polycephalum at S, G2, prophase, metaphase and ana-telophase were observed with an Hitachi electron microscope and gold particles marking the location of the SC35-..
- Structure of Physarum polycephalum cytochrome b5 reductase at 1.56 A resolutionSangwoo Kim
Institute for Protein Research, Osaka University, 3 2 Yamada Oka, Suita, Osaka, Japan
Acta Crystallogr Sect F Struct Biol Cryst Commun 63:274-9. 2007b>Physarum polycephalum cytochrome b(5) reductase catalyzes the reduction of cytochrome b(5) by NADH. The structure of P. polycephalum cytochrome b(5) reductase was determined at a resolution of 1.56 A...
- RNA editing of 10 Didymium iridis mitochondrial genes and comparison with the homologous genes in Physarum polycephalumStephen J Traphagen
The English High School, Boston Public Schools, Boston, Massachusetts 02130, USA
RNA 16:828-38. 2010..of editing events and editing sequence features were very similar to those observed in the homologous genes of Physarum polycephalum, though the actual editing locations showed a variable degree of conservation...
- Physarum amoebae express a distinct fragmin-like actin-binding protein that controls in vitro phosphorylation of actin by the actin-fragmin kinaseJ Bailey
Flanders Interuniversity Institute for Biotechnology VIB, Department of Medical Protein Research, Faculty of Medicine, University of Gent, Belgium
Eur J Biochem 265:240-50. 1999Amoebae and plasmodia constitute the two vegetative growth phases of the Myxomycete Physarum. In vitro and in vivo phosphorylation of actin in plasmodia is tightly controlled by fragmin P, a plasmodium-specific actin-binding protein that ..
- Platelet-activating factor acetylhydrolases: broad substrate specificity and lipoprotein binding does not modulate the catalytic properties of the plasma enzymeJ H Min
Department of Biochemistry and Chemistry, University of Washington, Seattle, Washington 98195, USA
Biochemistry 40:4539-49. 2001..homologous PAF-AH which occurs intracellularly [PAF-AH(II)] as well as for the PAF-AH from the lower eukaryote Physarum polycephalum although pPAF-AH and PAF-AH(II) tolerate the removal of the sn-3 headgroup better than the PAF-AH ..
- Calcium-induced conformational changes in Leishmania infantum kinetoplastid membrane protein-11M A Fuertes
Facultad de Ciencias, Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Contoblanco, Spain
J Biol Inorg Chem 6:107-17. 2001..the existence of 67% similarity and 33% identity with the EF-hand of the plasmodia-specific 40-kDa protein from Physarum polycephalum...
- Localization of the newly initiated and processed ribosomal primary transcripts during the mitotic cycle in Physarum polycephalumG Pierron
Laboratoire Organisation Fonctionnelle du Noyau, UPR 9044, Institut de Recherches sur le Cancer, Villejuif, 94801, France
Exp Cell Res 229:407-20. 1996..prophasic disintegration of the nucleolus, during the "closed" mitosis of the naturally synchronous plasmodium of Physarum polycephalum...
- The complete DNA sequence of the mitochondrial genome of Physarum polycephalumH Takano
Department of Biological Science, Faculty of Science, Kumamoto University, Japan
Mol Gen Genet 264:539-45. 2001..No guide RNA-like sequences were observed in the mtDNA of P. polycephalum. Comparison with reported Physarum mtDNA sequences suggested that sites of RNA editing vary among strains...
- Properties, intracellular localization, and stage-specific expression of membrane-bound beta-glucosidase, BglM1, from Physarum polycephalumMasato Hayase
Department of Biochemistry, Okayama University of Science, 1 1 Ridai cho, Okayama 700 0005, Japan
Int J Biochem Cell Biol 40:2141-50. 2008b>Physarum polycephalum expresses a membrane-bound beta-glucosidase (BglM1) with a molecular mass of 130 kDa...
- Maternal inheritance of mitochondria: multipolarity, multiallelism and hierarchical transmission of mitochondrial DNA in the true slime mold Physarum polycephalumYohsuke Moriyama
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bldg FSB 601, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japane
J Plant Res 123:139-48. 2010Direct evidence of digestion of paternal mitochondrial DNA (mtDNA) has been found in the true slime mold Physarum polycephalum...
- Clathrin is involved in organization of mitotic spindle and phragmoplast as well as in endocytosis in tobacco cell culturesH Tahara
Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo, Japan
Protoplasma 230:1-11. 2007..in Lilium longiflorum germinating pollen using a monoclonal antibody raised against myosin II heavy chain from Physarum polycephalum. In the present study, the equivalent polypeptide was also found in cultured tobacco BY-2 cells...
- Nucleotide sequence of pig plasma gelsolin. Comparison of protein sequence with human gelsolin and other actin-severing proteins shows strong homologies and evidence for large internal repeatsM Way
MRC Laboratory of Molecular Biology, Cambridge, England
J Mol Biol 203:1127-33. 1988..the gelsolin sequences with chicken brush border villin, severin from Dictyostelium discoideum and fragmin from Physarum polycephalum shows a strong evolutionary relationship between all these proteins...
- Identification of a nuclear localization motif in the serine/arginine protein kinase PSRPK of physarum polycephalumShide Liu
Shenzhen Key Laboratory of Microbial Genetic Engineering and College of Life Science, Shenzhen University, Shenzhen, PR China
BMC Biochem 10:22. 2009..But no nuclear localization signal (NLS) of SRPKs was found. We isolated an SRPK-like protein PSRPK (GenBank accession No. DQ140379) from Physarum polycephalum previously, and identified a NLS of PSRPK in this study.
- Glom is a novel mitochondrial DNA packaging protein in Physarum polycephalum and causes intense chromatin condensation without suppressing DNA functionsNarie Sasaki
Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan
Mol Biol Cell 14:4758-69. 2003..inducing agglomeration of mitochondrial chromosome), from highly condensed mt-nucleoids of the true slime mold, Physarum polycephalum...
- Non-DNA-templated addition of nucleotides to the 3' end of RNAs by the mitochondrial RNA polymerase of Physarum polycephalumMara L Miller
Department of Molecular and Cell Biology, The University of Texas at Dallas, Box 830688, Richardson, TX 75083 0688, USA
Mol Cell Biol 28:5795-802. 2008..In the myxomycete Physarum polycephalum, nucleotides that are not specified by the mitochondrial DNA templates are inserted into some RNAs, ..
- Early activated replication origins within the cell cycle-regulated histone H4 genes in PhysarumM Benard
Laboratoire Organisation Fonctionnelle du Noyau, CNRS UPR 1983, IFR 1221, F 94801 Villejuif, France
Nucleic Acids Res 27:2091-8. 1999..H4 gene family, H4-1 and H4-2, are replicated at the onset of S phase in the naturally synchronous plasmodium of Physarum polycephalum, suggesting that they are flanked by replication origins...
- Two forms of RNA editing are required for tRNA maturation in Physarum mitochondriaJonatha M Gott
Center for RNA Molecular Biology, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
RNA 16:482-8. 2010The mitochondrial genome of Physarum polycephalum encodes five tRNAs, four of which are edited by nucleotide insertion...
- A novel type of protein kinase phosphorylates actin in the actin-fragmin complexL Eichinger
Adolf Butenandt Institut Zellbiologie, Ludwig Maximilians Universitat, Munchen, Germany
EMBO J 15:5547-56. 1996Actin-fragmin kinase (AFK) from Physarum polycephalum specifically phosphorylates actin in the EGTA-resistant 1:1 actin-fragmin complex...
- Invalidation of Hyperamoeba by transferring its species to other genera of MyxogastriaAnna Maria Fiore-Donno
Zoology Department, University of Oxford, South Parks Road, OX13PS Oxford, United Kingdom
J Eukaryot Microbiol 57:189-96. 2010..Phylogenetic analyses show unambiguously that the type species Hyperamoeba flagellata is a Physarum (Physarum flagellatum comb. nov...
- Regulation of levels of actin threonine phosphorylation during life cycle of Physarum polycephalumYuki Shirai
Department of Biology, Ochanomizu University, Bunkyo ku, Tokyo 112 8610, Japan
Cell Motil Cytoskeleton 63:77-87. 2006Under various environmental stresses, the true slime mold Physarum polycephalum converts into dormant forms, such as microcysts, sclerotia, and spores, which can survive in adverse environments for a considerable period of time...
- The crystal structure of the Physarum polycephalum actin-fragmin kinase: an atypical protein kinase with a specialized substrate-binding domainS Steinbacher
Abteilung Strukturforschung, Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
EMBO J 18:2923-9. 1999..In plasmodia of Physarum polycephalum, the F-actin capping activity of the actin-fragmin complex is regulated by the phosphorylation of ..
- G1-phase arrest is not a prerequisite for encystment in PhysarumR W Anderson
Department of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom
Exp Cell Res 236:351-4. 1997Amoebae of the Myxomycete Physarum polycephalum form resistant, walled cysts when the food bacteria in a culture have been consumed. No G1 phase has been detected in the vegetative amoebal cell cycle, most of which comprises the G2 phase...
- Spatiotemporal symmetry in rings of coupled biological oscillators of Physarum plasmodial slime moldA Takamatsu
PRESTO, Japan Science and Technology Corporation, Saitama, Japan
Phys Rev Lett 87:078102. 2001Spatiotemporal patterns in rings of coupled biological oscillators of the plasmodial slime mold, Physarum polycephalum, were investigated by comparing with results analyzed by the symmetric Hopf bifurcation theory based on group theory...
- Chromatin remodeling by cell cycle stage-specific extracts from Physarum polycephalumC Thiriet
Department of Biochemistry and Biophysics, University of Rochester Medical Center, NY USA
Eur J Cell Biol 78:214-20. 1999..The slime mold Physarum polycephalum presents the attractive advantage of natural synchrony of the cell cycle in several million nuclei...
- RNase-dependent discontinuities associated with the crossovers of spontaneously formed joint DNA molecules in Physarum polycephalumChrystelle Maric
Laboratoire Réplication de l ADN et Ultrastructure du Noyau, CNRS FRE 3238, Institut Andre Lwoff, 7 rue Guy Moquet, BP 8, 94801, Villejuif Cedex, France
Chromosoma 119:601-11. 2010..Here, we studied their structure and frequency of formation in Physarum polycephalum. By "3D gels", we evidenced that they are not made of four continuous DNA strands...
- Physarum polymalic acid hydrolase: Recombinant expression and enzyme activationWolfgang Mueller
Biophysik und Physikalische Biochemie, Universitaet Regensburg, 93040 Regensburg, Germany
Biochem Biophys Res Commun 377:735-40. 2008..acid together with its tailoring specific exohydrolase is purified from plasmodium cultures of the slime mold Physarum polycephalum, a member of the phylum myxomycota...
- RNA B is the major nucleolar trimethylguanosine-capped small nuclear RNA associated with fibrillarin and pre-rRNAs in Trypanosoma bruceiT Hartshorne
Intercampus Program in Molecular Parasitology, School of Pharmacy, University of California, San Francisco 94143 1204
Mol Cell Biol 13:144-54. 1993..A 35-kDa T. brucei fibrillarin homolog was identified by using an anti-Physarum fibrillarin monoclonal antibody...
- Dry stress-induced phosphorylation of Physarum actinK Furuhashi
Department of Biology, Faculty of Education, Shiga University, Japan
Biochem Biophys Res Commun 242:653-8. 1998Protein phosphorylation plays important roles in a variety of stress responses. Although plasmodium of Physarum polycephalum rapidly grows and shows an active cytoplasmic streaming under nutrient and wet conditions, dry stress transforms ..
- Evolution of a protein superfamily: relationships between vertebrate lens crystallins and microorganism dormancy proteinsG Wistow
National Eye Institute, National Institutes of Health, Bethesda, Maryland 20982
J Mol Evol 30:140-5. 1990A search of sequence databases shows that spherulin 3a, an encystment-specific protein of Physarum polycephalum, is probably structurally related to the beta- and gamma-crystallins, vertebrate ocular lens proteins, and to Protein S, a ..
- Linker histone phosphorylation regulates global timing of replication origin firingChristophe Thiriet
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
J Biol Chem 284:2823-9. 2009..Here, we show that linker histone (H1) is involved in the global regulation of DNA replication in Physarum polycephalum...
- Generation of highly site-specific DNA double-strand breaks in human cells by the homing endonucleases I-PpoI and I-CreIR J Monnat
Department of Pathology, University of Washington, Seattle, Washington 98195, USA
Biochem Biophys Res Commun 255:88-93. 1999..have determined the ability of two well-characterized eukaryotic homing endonucleases, I-PpoI from the myxomycete Physarum polycephalum and I-CreI from the green alga Chlamydomonas reinhardtii, to generate site-specific DNA double-..
- Amoeboid organism solves complex nutritional challengesAudrey Dussutour
Centre de Recherches sur la Cognition Animale, Universite Paul Sabatier, 31062 Toulouse, France
Proc Natl Acad Sci U S A 107:4607-11. 2010..organisms coordinate the search for multiple nutrients? We address this question in the acellular slime mold Physarum polycephalum and show that this extraordinary organism can make complex nutritional decisions, despite lacking a ..
- Biological functions of a novel lipid mediator, cyclic phosphatidic acidKimiko Murakami-Murofushi
Department of Biology, Faculty of Science, Ochanomizu University, 2 1 1 Ohtsuka, Bunkyo ku, 112 8610, Tokyo, Japan
Biochim Biophys Acta 1582:1-7. 2002..bioactive lipid, cyclic phosphatidic acid (cPA), was isolated originally from myxoamoebae of a true slime mold, Physarum polycephalum, and has now been detected in a wide range of organisms from slime molds to humans...
- A first glimpse at the transcriptome of Physarum polycephalumGernot Glockner
Leibniz Institute for Age Research Fritz Lipmann Institute, Beutenbergstr, 11, D 07745 Jena, Germany
BMC Genomics 9:6. 2008b>Physarum polycephalum, an acellular plasmodial species belongs to the amoebozoa, a major branch in eukaryote evolution...
- [Transient expression in microplasmodia of Physarum polycephalum]Shide Liu
Shenzhen Key Laboratory of Microbial and Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China
Sheng Wu Gong Cheng Xue Bao 25:854-62. 2009The plasmodium of Physarum polycephalum is a suitable eukaryotic cell for cell cycle investigation, but there is no compatible transient expression system for the plasmodium...
- Transcriptomic changes arising during light-induced sporulation in Physarum polycephalumIsrael Barrantes
Max Planck Institute for Dynamics of Complex Technical Systems and Magdeburg Centre for Systems Biology MaCS, Otto von Guericke University, Magdeburg, Germany
BMC Genomics 11:115. 2010b>Physarum polycephalum is a free-living amoebozoan protist displaying a complex life cycle, including alternation between single- and multinucleate stages through sporulation, a simple form of cell differentiation...
- A novel Physarum polycephalum SR protein kinase specifically phosphorylates the RS domain of the human SR protein, ASF/SF2Shide Liu
Shenzhen Key Laboratory of Microbial and Genetic Engineering, College of Life Science Shenzhen University, Shenzhen, China
Acta Biochim Biophys Sin (Shanghai) 41:657-67. 2009A 1591-bp cDNA of a serine-rich protein kinase (SRPK)-like protein has been identified in Physarum polycephalum (GenBank accession No. DQ140379). The cDNA contains two repeat sequences at bp 1-153 and bp 395-547...
- Physarum nitric oxide synthases: genomic structures and enzymology of recombinant proteinsSimon Messner
Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3 VI, Innsbruck, Austria
Biochem J 418:691-700. 2009b>Physarum polycephalum expresses two closely related, calcium-independent NOSs (nitric oxide synthases). In our previous work, we showed that both NOSs are induced during starvation and apparently play a functional role in sporulation...
- The one-kilobase DNA fragment upstream of the ardC actin gene of Physarum polycephalum is both a replicator and a promoterG Pierron
Laboratoire Organisation Fonctionnelle du Noyau, UPR 9044, CNRS, Institut de Recherches sur le Cancer, 94801 Villejuif, France
Mol Cell Biol 19:3506-14. 1999The 1-kb DNA fragment upstream of the ardC actin gene of Physarum polycephalum promotes the transcription of a reporter gene either in a transient-plasmid assay or as an integrated copy in an ectopic position, defining this region as the ..
- A calmodulin-dependent protein kinase from lower eukaryote Physarum polycephalumAkio Nakamura
Department of Molecular and Cellular Pharmacology, Faculty of Medicine, Gunma University Graduate School of Medicine, Gunma 371 8511, Japan
Biochem Biophys Res Commun 328:838-44. 2005A full-length cDNA coding a calmodulin (CaM)-dependent protein kinase gene was cloned from Physarum plasmodia poly(A)-RNA by polymerase chain reaction with the oligonucleotide primers that were designed after the amino acid sequence of ..
- Characteristics of pattern formation and evolution in approximations of Physarum transport networksJeff Jones
Centre for Unconventional Computing, University of the West of England, Bristol, BS16 1QY, UK
Artif Life 16:127-53. 2010..Inspired by one such organism, the true slime mold Physarum polycephalum, we present examples of complex emergent pattern formation and evolution formed by a population of ..
- Distinct roles for sequences upstream of and downstream from Physarum editing sitesAmy C Rhee
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
RNA 15:1753-65. 2009RNAs in the mitochondria of Physarum polycephalum contain nonencoded nucleotides that are added during RNA synthesis...
- Action spectrum for sporulation and photoavoidance in the plasmodium of Physarum polycephalum, as modified differentially by temperature and starvationT Nakagaki
Graduate School of Human Informatics, Nagoya University, Japan
Photochem Photobiol 64:859-62. 1996The plasmodium of the myxomycete Physarum polycephalum sporulates in bright natural environments, suggesting a relationship between photobehavior and sporulation...
- Purification and partial characterization of a nucleolar scleroderma antigen (Mr = 34,000; pI, 8.5) rich in NG,NG-dimethylarginineM A Lischwe
J Biol Chem 260:14304-10. 1985..In the first 31 residues, there are 16 glycine, 6 DMA, and 3 phenylalanine residues. This is a novel demonstration of clusters of glycine and DMA in a protein...
- Polyamines in microorganismsC W Tabor
Microbiol Rev 49:81-99. 1985
- Cellular memory hints at the origins of intelligencePhilip Ball
Nature 451:385. 2008
- Determination of absolute stereochemistry, total synthesis, and evaluation of peptides from the myxomycete Physarum melleumShuwa Hanazawa
Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 263 8522, Japan
Bioorg Med Chem Lett 18:95-8. 2008The absolute stereochemistry of melleumin A (1) and B (2), novel peptide compounds isolated from the myxomycete Physarum melleum, was determined by synthesis of their segments and by a modified Mosher's method...
- [Isolation and characterization of the ALP1 protease from Aspergillus fumigatus and its protein inhibitor from Physarium polycephalum]D A Davies
Bioorg Khim 31:259-68. 2005..A thermostable protein inhibitor of the ALP1 protease was isolated from the plasmodium of the myxomycete Physarum polycephalum. Its molecular mass is 32-33 kDa. The inhibitor inhibits the ALP1 protease activity with IC50 of 0...
- Nanoconjugate based on polymalic acid for brain tumor treatmentJulia Y Ljubimova; Fiscal Year: 2013..system (Polycefin) based on naturally derived nanoplatform, poly(p-L-malic acid) (PMLA) fi-om a slime mold, Physarum polycephalum...
- REGULATION OF ORNITHINE DECARBOXYLASE ACTIVITYJohn Mitchell; Fiscal Year: 1980..of the regulation of this enzyme will be performed on the readily available enzyme from the primitive eukaryote, Physarum polycephalum, while a similar investigation, initially in less detail, will be made of the two enzyme forms in ..
- ULTRASTRUCTURAL STUDIES OF GENETIC ACTIVITYOscar Miller; Fiscal Year: 1992..interactions will be studied in eukaryotes using synchronously replicating yeast cells, Drosophila embryos and Physarum cultures...
- PHYSICAL STUDIES OF CONFORMATIONAL CHANGE IN CHROMATINRODNEY HARRINGTON; Fiscal Year: 1990..to characterize the structure and conformation of chromatin from the naturally mitotically synchronous slime mold Physarum polycephalum...
- RIBOSOMAL DNA IN PHYSARUM POLYCEPHALUMVOLKER VOGT; Fiscal Year: 1990The gene coding for ribosomal RNA in Physarum polycephalum exist as a collection of about 200 extrachromosomal DNA molecules 60 kb in size. This rDNA can be purified readily; most of it has been cloned and sequenced...
- MITOCHONDRIAL GENE EXPRESSION IN PHYSARUM POLYCEPHALUMDennis Miller; Fiscal Year: 1992..A unique step in mitochondrial gene expression has been identified in the mitochondria of the myxomycot, Physarum polycephalum...
- THE MECHANISM OF RADIATION-INDUCED MITOTIC DELAYNANCY OLEINICK; Fiscal Year: 1980..We are taking advantage of the natural synchrony of nuclear division in the plasmodial phase of Physarum polycephalum, in which we previously determined the times in late G2-early prophase when the plasmodium loses ..
- Transcription and Insertional editing in PhysarumJonatha Gott; Fiscal Year: 2006..The long-term goal of this project is the elucidation of the mechanism of insertional RNA editing in Physarum mitochondria...
- MEMBRANE STRUCTURE, CELL CONTACT AND CELL FUSIONHenry Aldrich; Fiscal Year: 1980Myxamoebae of Didymium iridis, Physarum polycephalum, Physarum flavicomum, and Didtypostelium discoideum will be studied to determine the relation of cell surface and membrane structure to sexual fusion processes...
- Tumor drug delivery systems based on polymalic acidJulia Y Ljubimova; Fiscal Year: 2010..A new nanoscale drug delivery system (drug size 20-30 nm) based on naturally derived poly malic acid (PMLA) from Physarum polycephalum will be studied...
- NOVEL HUMAN GENE SPECIFIC REAGENTS FROM HOMING ENDOSRaymond Monnat; Fiscal Year: 2005..The availability of human GSR's may also allow modification of altered human genes responsible for specific diseases or genes of pathogenic microorganisms including bacteria and viruses, parasites, etc. ..