physarum

Summary

Summary: A genus of protozoa, formerly also considered a fungus. Characteristics include the presence of violet to brown spores.

Top Publications

  1. ncbi Effects of Ca2+-ATPase inhibitors, ionomycin, and pharmacological modulators of ryanodine receptor on calcium release from intracellular pools and on oscillatory contractile behavior in Physarum polycephalum
    A A Kochegarov
    Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia
    Biochemistry (Mosc) 65:662-71. 2000
  2. ncbi Flow-network adaptation in Physarum amoebae
    Atsushi Tero
    Research Institute for Electronic Science, Hokkaido University, Sapporo, 060 0812, Japan
    Theory Biosci 127:89-94. 2008
  3. ncbi Nucleotide sequence of the Physarum polycephalum small subunit ribosomal RNA as inferred from the gene sequence: secondary structure and evolutionary implications
    T Johansen
    Institute of Medical Biology, University of Tromsø, Norway
    Curr Genet 14:265-73. 1988
  4. ncbi Structure and evolution of myxomycete nuclear group I introns: a model for horizontal transfer by intron homing
    S Johansen
    Institute of Medical Biology, University of Tromsø, Norway
    Curr Genet 22:297-304. 1992
  5. ncbi Extrachromosomal ribosomal DNA of Didymium iridis: sequence analysis of the large subunit ribosomal RNA gene and sub-telomeric region
    S Johansen
    Institute of Medical Biology, University of Tromsø, Norway
    Curr Genet 22:305-12. 1992
  6. ncbi Selective inhibition of DNA polymerase-alpha family with chemically synthesized derivatives of PHYLPA, a unique Physarum lysophosphatidic acid
    K Murakami-Murofushi
    Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan
    Biochim Biophys Acta 1258:57-60. 1995
  7. pmc Insertional editing of nascent mitochondrial RNAs in Physarum
    L M Visomirski-Robic
    Department of Molecular Biology and Microbiology, Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 94:4324-9. 1997
  8. ncbi Constitutive homologous recombination between mitochondrial DNA and a linear mitochondrial plasmid in Physarum polycephalum
    H Takano
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    Curr Genet 22:221-7. 1992
  9. ncbi RNA editing in Physarum mitochondria: assays and biochemical approaches
    Elaine M Byrne
    Trinity Centre for Engineering, Trinity College Dublin, Dublin, Ireland
    Methods Enzymol 424:143-72. 2007
  10. ncbi Identification of proteins immunologically related to vertebrate lamins in the nuclear matrix of the myxomycete Physarum polycephalum
    S Lang
    Department of Microbiology, University of Innsbruck Medical School, Austria
    Eur J Cell Biol 61:177-83. 1993

Research Grants

Detail Information

Publications168 found, 100 shown here

  1. ncbi Effects of Ca2+-ATPase inhibitors, ionomycin, and pharmacological modulators of ryanodine receptor on calcium release from intracellular pools and on oscillatory contractile behavior in Physarum polycephalum
    A A Kochegarov
    Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia
    Biochemistry (Mosc) 65:662-71. 2000
    Changes in calcium levels in organelles of the plasmodium of the myxomycete Physarum polycephalum were analyzed using the fluorescent calcium indicator chlortetracycline (CTC)...
  2. ncbi Flow-network adaptation in Physarum amoebae
    Atsushi Tero
    Research Institute for Electronic Science, Hokkaido University, Sapporo, 060 0812, Japan
    Theory Biosci 127:89-94. 2008
    ..The large amoeboid plasmodium of Physarum is able to solve a maze and to connect multiple food locations via a smart network...
  3. ncbi Nucleotide sequence of the Physarum polycephalum small subunit ribosomal RNA as inferred from the gene sequence: secondary structure and evolutionary implications
    T Johansen
    Institute of Medical Biology, University of Tromsø, Norway
    Curr Genet 14:265-73. 1988
    The nucleotide sequence of the Physarum polycephalum small subunit ribosomal RNA (SSU rRNA) gene has been determined. Sequence data indicate that the mature 19S SSU rRNA is 1,964 nucleotides long...
  4. ncbi Structure and evolution of myxomycete nuclear group I introns: a model for horizontal transfer by intron homing
    S Johansen
    Institute of Medical Biology, University of Tromsø, Norway
    Curr Genet 22:297-304. 1992
    ..positions in the large subunit ribosomal RNA (LSU rRNA) gene of the two myxomycete species, Didymium iridis and Physarum polycephalum. Structural models of intron RNAs, including secondary and tertiary interactions, are proposed...
  5. ncbi Extrachromosomal ribosomal DNA of Didymium iridis: sequence analysis of the large subunit ribosomal RNA gene and sub-telomeric region
    S Johansen
    Institute of Medical Biology, University of Tromsø, Norway
    Curr Genet 22:305-12. 1992
    ..Based on pairwise comparisons of LSU rRNA sequences, the time of divergence between the two myxomycete genera Didymium and Physarum was estimated.
  6. ncbi Selective inhibition of DNA polymerase-alpha family with chemically synthesized derivatives of PHYLPA, a unique Physarum lysophosphatidic acid
    K Murakami-Murofushi
    Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan
    Biochim Biophys Acta 1258:57-60. 1995
    PHYLPA, a unique Physarum lysophosphatidic acid (LPA), showed selective inhibition of a family of DNA polymerase alpha, including DNA polymerases alpha, delta and epsilon; but no inhibition of DNA polymerase beta or gamma was observed...
  7. pmc Insertional editing of nascent mitochondrial RNAs in Physarum
    L M Visomirski-Robic
    Department of Molecular Biology and Microbiology, Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 94:4324-9. 1997
    Maturation of Physarum mitochondrial RNA involves the highly specific insertion of nonencoded nucleotides at multiple locations...
  8. ncbi Constitutive homologous recombination between mitochondrial DNA and a linear mitochondrial plasmid in Physarum polycephalum
    H Takano
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    Curr Genet 22:221-7. 1992
    In one particular myxamoebal strain (NG7; mF+) of Physarum polycephalum, a linear mitochondrial plasmid (mF plasmid) which promotes mitochondrial fusion has been identified...
  9. ncbi RNA editing in Physarum mitochondria: assays and biochemical approaches
    Elaine M Byrne
    Trinity Centre for Engineering, Trinity College Dublin, Dublin, Ireland
    Methods Enzymol 424:143-72. 2007
    Mitochondrial RNAs in the myxomycete Physarum polycephalum differ from the templates from which they are transcribed in defined ways. Most transcripts contain nucleotides that are not present in their respective genes...
  10. ncbi Identification of proteins immunologically related to vertebrate lamins in the nuclear matrix of the myxomycete Physarum polycephalum
    S Lang
    Department of Microbiology, University of Innsbruck Medical School, Austria
    Eur J Cell Biol 61:177-83. 1993
    Lamin-like proteins have been identified as components of the nuclear matrix of the myxomycete Physarum polycephalum...
  11. ncbi Specificity and direction of depolymerization of beta-poly(L-malate) catalysed by polymalatase from Physarum polycephalum--fluorescence labeling at the carboxy-terminus of beta-poly(L-malate)
    B Gasslmaier
    Institut für Biophysik und physikalische Biochemie der Universität Regensburg, Germany
    Eur J Biochem 250:308-14. 1997
    Beta-poly(L-malate), a major constituent of nuclei in plasmodia of Physarum polycephalum, is enzymatically degraded to L-malate after secretion into the culture medium...
  12. pmc Replication timing of 10 developmentally regulated genes in Physarum polycephalum
    G Pierron
    Institut de Recherches Scientifiques sur le Cancer, Villejuif, France
    Nucleic Acids Res 17:553-66. 1989
    ..Within one cell type of Physarum - the plasmodium - we defined the temporal order of replication of 10 genes which were known to be variably ..
  13. pmc Polyphyletic origin of the genus Physarum (Physarales, Myxomycetes) revealed by nuclear rDNA mini-chromosome analysis and group I intron synapomorphy
    Satish C R Nandipati
    RNA and Transcriptomics Group, Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, MH building Breivika, N 9037, Tromsø, Norway
    BMC Evol Biol 12:166. 2012
    ..Physarales is of particular interest since the two best-studied myxomycete species, Physarum polycephalum and Didymium iridis, belong to this order and are currently subjected to whole genome and ..
  14. pmc Identification of the telomeric sequence of the acellular slime molds Didymium iridis and Physarum polycephalum
    J Forney
    Department of Molecular Biology, University of California, Berkeley 94720
    Nucleic Acids Res 15:9143-52. 1987
    We have determined the telomeric DNA sequence of the acellular slime molds Didymium iridis and Physarum polycephalum. In both organisms the telomeres consist of tandem repeats of the hexamer 5'(TTAGGG)3'...
  15. ncbi Structure of the extrachromosomal ribosomal RNA chromatin of Physarum polycephalum
    R Lucchini
    Institut für Zellbiologie ETH Hönggerberg, Zurich, Switzerland
    J Mol Biol 196:829-43. 1987
    Isolated nucleoli from exponentially growing microplasmodia of Physarum polycephalum were digested with micrococcal nuclease or DNAase I, or were photoreacted with trimethyl psoralen...
  16. pmc Purification of tropomyosin from Saccharomyces cerevisiae and identification of related proteins in Schizosaccharomyces and Physarum
    H P Liu
    Section of Biochemistry, Cornell University, Ithaca, NY 14853
    Proc Natl Acad Sci U S A 86:90-3. 1989
    ..specifically crossreact with heat-stable Mr 33,000 polypeptides in extracts of Schizosaccharomyces pombe and Physarum polycephalum...
  17. pmc Non-templated addition of nucleotides to the 3' end of nascent RNA during RNA editing in Physarum
    Y W Cheng
    Center for RNA Molecular Biology, Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    EMBO J 20:1405-14. 2001
    RNAs in Physarum: mitochondria contain extra nucleotides that are not encoded by the mitochondrial genome, at least in the traditional sense...
  18. ncbi Mitochondrial DNA of Physarum polycephalum: physical mapping, cloning and transcription mapping
    E P Jones
    Cell and Molecular Biology Program, University of Texas, Dallas, Richardson 75083 0688
    Curr Genet 17:331-7. 1990
    Mitochondrial DNA (mtDNA) has been isolated from four strains of Physarum polycephalum and a restriction site map has been determined using nine restriction enzymes...
  19. ncbi RNA editing by cytidine insertion in mitochondria of Physarum polycephalum
    R Mahendran
    Cell and Molecular Biology Program, University of Texas at Dallas, Richardson 75083 0688
    Nature 349:434-8. 1991
    ..The mitochondrial RNA apparently encoding the alpha subunit of ATP synthetase in the acellular slime mould, Physarum polycephalum, is edited at 54 sites by cytidine insertion.
  20. pmc Insertional editing of mitochondrial tRNAs of Physarum polycephalum and Didymium nigripes
    T Antes
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75080, USA
    Mol Cell Biol 18:7521-7. 1998
    tRNAs encoded on the mitochondrial DNA of Physarum polycephalum and Didymium nigripes require insertional editing for their maturation...
  21. ncbi Gene families encode the major encystment-specific proteins of Physarum polycephalum plasmodia
    F Bernier
    Departement de Biochimie, Faculte des sciences et de genie, Universite Laval, Quebec, Canada
    Gene 59:265-77. 1987
    The encystment of Physarum polycephalum plasmodia, also called spherulation, involves the synthesis of many specific mRNAs and proteins...
  22. pmc Nucleolar introns from Physarum flavicomum contain insertion elements that may explain how mobile group I introns gained their open reading frames
    A Vader
    Department of Cell Biology, University of Tromsø, Norway
    Nucleic Acids Res 22:4553-9. 1994
    Comparison of two group I intron sequences in the nucleolar genome of the myxomycete Physarum flavicomum to their homologs in the closely related Physarum polycephalum revealed insertion-like elements...
  23. ncbi Involvement of actin dephosphorylation in germination of Physarum sclerotium
    Kiyoshi Furuhashi
    Department of Biology, Faculty of Education, Shiga University, Otsu, Japan
    J Eukaryot Microbiol 49:129-33. 2002
    The plasmodium of Physarum polycephalum grows without cytokinesis and shows an active cytoplasmic streaming under wet and nutritious conditions...
  24. pmc Purification and partial characterization of transglutaminase from Physarum polycephalum
    J D Klein
    Graduate Program in Molecular Biology, New Mexico State University, Las Cruces 88003 0001
    J Bacteriol 174:2599-605. 1992
    ..818-fold to apparent homogeneity from acetone powder preparations of spherules of the acellular slime mold Physarum polycephalum...
  25. ncbi Methylation is co-ordinated on the putative replication origins of Physarum ribosomal DNA
    C A Cooney
    Department of Biological Chemistry, School of Medicine, University of California, Davis 95616
    J Mol Biol 204:889-901. 1988
    In Physarum polycephalum, the ribosomal DNA is found as 60,000 base-pair palindromes. Each rDNA has four symmetrically arranged replication origins flanked by ribosomal RNA genes...
  26. pmc Dynamic organization of ATP and birefringent fibrils during free locomotion and galvanotaxis in the plasmodium of Physarum polycephalum
    T Ueda
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    J Cell Biol 110:1097-102. 1990
    ..of both ATP and birefringent fibrils throughout the cell was studied in the multinuclear ameboid cell of the Physarum plasmodium during free locomotion and galvanotaxis...
  27. ncbi A mobile group I intron in the nuclear rDNA of Physarum polycephalum
    D E Muscarella
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    Cell 56:443-54. 1989
    We have shown that a strain-specific group I intron (intron 3) in the nuclear extrachromosomal rDNA or Physarum polycephalum is a mobile element...
  28. pmc Retrotransposon-like nature of Tp1 elements: implications for the organisation of highly repetitive, hypermethylated DNA in the genome of Physarum polycephalum
    H M Rothnie
    Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, UK
    Nucleic Acids Res 19:279-86. 1991
    The repetitive fraction of the genome of the eukaryotic slime mould Physarum polycephalum is dominated by the Tp1 family of highly repetitive retrotransposon-like sequences...
  29. pmc Selective gene expression during sporulation of Physarum polycephalum
    R Martel
    Department of Biochemistry, Faculty of Sciences, Laval University, Quebec, Canada
    J Bacteriol 170:4784-90. 1988
    ..of polypeptides synthesized in vitro from poly(A)+ RNA showed that mRNA populations change during sporulation of Physarum polycephalum...
  30. pmc Characterization of I-Ppo, an intron-encoded endonuclease that mediates homing of a group I intron in the ribosomal DNA of Physarum polycephalum
    D E Muscarella
    Section of Biochemistry, Molecular and Cellular Biology, Cornell University, Ithaca, New York 14853
    Mol Cell Biol 10:3386-96. 1990
    ..several aspects of I-Ppo, the endonuclease that mediates the mobility of intron 3 in the ribosomal DNA of Physarum polycephalum. This intron is unique among mobile group I introns in that it is located in nuclear DNA...
  31. ncbi Physical relationship between a gene and its origin of replication in Physarum polycephalum
    M Benard
    Laboratoire de Biologie et Ultrastructure du Noyau, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France
    Exp Cell Res 186:299-305. 1990
    Taking advantage of the natural synchrony of the S-phase within the plasmodium of Physarum polycephalum, we extracted highly synchronous DNA samples at precise time points in early S-phase...
  32. pmc Site-specific initiation of DNA replication within the non-transcribed spacer of Physarum rDNA
    M Benard
    Organisation Fonctionnelle du Noyau, UPR 9044 CNRS, Villejuif, France
    Nucleic Acids Res 23:1447-53. 1995
    b>Physarum polycephalum rRNA genes are found on extrachromosomal 60 kb linear palindromic DNA molecules...
  33. pmc Changes in diadenosine tetraphosphate levels in Physarum polycephalum with different oxygen concentrations
    P N Garrison
    Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284 7760
    J Bacteriol 171:1506-12. 1989
    ..Ap4A) were measured, by a specific high-pressure liquid chromatography method, in microplasmodia of Physarum polycephalum subjected to different degrees of hypoxia, hyperoxia, and treatment with H2O2...
  34. ncbi Immunoelectron microscopic localization of the nucleolar protein B-36 (fibrillarin) during the cell cycle of Physarum polycephalum
    G Pierron
    Laboratoire de Biologie et Ultrastructure du Noyau, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France
    Biol Cell 65:119-26. 1989
    Monoclonal antibodies raised against the 34-kD nucleolar protein, B-36, from the slime mold Physarum polycephalum have been used to examine the electron microscopic localization of B-36 during the cell cycle in Physarum...
  35. pmc The F-actin capping proteins of Physarum polycephalum: cap42(a) is very similar, if not identical, to fragmin and is structurally and functionally very homologous to gelsolin; cap42(b) is Physarum actin
    C Ampe
    Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium
    EMBO J 6:4149-57. 1987
    We have carried out a primary structure analysis of the F-actin capping proteins of Physarum polycephalum. Cap42(b) was completely sequenced and was found to be identical with Physarum actin...
  36. pmc Locomotive mechanism of Physarum plasmodia based on spatiotemporal analysis of protoplasmic streaming
    Kenji Matsumoto
    Department of Mathematics, Hokkaido University, Sapporo, Japan
    Biophys J 94:2492-504. 2008
    We investigate how an amoeba mechanically moves its own center of gravity using the model organism Physarum plasmodium...
  37. ncbi Actin co-purifies with RNA polymerase II
    S S Smith
    Biochem Biophys Res Commun 86:161-6. 1979
  38. ncbi Fragmin60 encodes an actin-binding protein with a C2 domain and controls actin Thr-203 phosphorylation in Physarum plasmodia and sclerotia
    Tatyana Sklyarova
    Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Rommelaere Institute, Albert Baertsoenkaai 3, B 9000 Ghent, Belgium
    J Biol Chem 277:39840-9. 2002
    ..show that in vivo phosphorylation of actin in the fragmin60-actin complex occurs in sclerotia, a dormant stage of Physarum development, as well as in plasmodia...
  39. ncbi Ca2+-binding modulator protein in protozoa and myxomycete
    J Kuznicki
    Cell Biol Int Rep 3:17-23. 1979
    ..with the properties of brain modulator protein of 3,5-cyclic nucleotide phosphodiesterase was identified in Physarum polycephalum plasmodia and in Euglena gracilis and Amoeba proteus cells by urea polyacrylamide gel ..
  40. ncbi Exon junction sequences as cryptic splice sites: implications for intron origin
    Terrie Sadusky
    Institute of Reproductive and Developmental Biology, Hammersmith Campus, Imperial College London, London W12 ONN, England
    Curr Biol 14:505-9. 2004
    ..identified nine cryptic splice sites within the coding sequence of actin genes from humans, Arabidopsis, and Physarum by inactivating their normal intron splice sites...
  41. ncbi Physarum machines: encapsulating reaction-diffusion to compute spanning tree
    Andrew Adamatzky
    Faculty of Computing, Engineering and Mathematical Sciences, University of the West of England, Bristol, UK
    Naturwissenschaften 94:975-80. 2007
    The Physarum machine is a biological computing device, which employs plasmodium of Physarum polycephalum as an unconventional computing substrate...
  42. ncbi Structural evidence for non-canonical binding of Ca2+ to a canonical EF-hand of a conventional myosin
    Judit E Debreczeni
    Department of Biochemistry, Eotvos Lorand University, Budapest H 1117, Hungary
    J Biol Chem 280:41458-64. 2005
    ..previously identified a single inhibitory Ca2+-binding site in the first EF-hand of the essential light chain of Physarum conventional myosin (Farkas, L., Malnasi-Csizmadia, A., Nakamura, A., Kohama, K., and Nyitray, L. (2003) J. Biol...
  43. ncbi Polypropionate lactones of deoxysugars glycosides from slime mold Lycogala epidendrum
    Tomas Rezanka
    Institute of Microbiology, Videnska 1083, 14220 Prague, Czech Republic
    Phytochemistry 63:945-52. 2003
    ....
  44. ncbi Specific and nonspecific enzymes involved in the catabolism of mononucleoside and dinucleoside polyphosphates
    A Guranowski
    Katedra Biochemii i Biotechnologii, Akademia Rolnicza, ul Wo gt yOska 35, 60 637, PoznaO, Poland
    Pharmacol Ther 87:117-39. 2000
    ..asymmetrically cleaving ones, which are typical of higher eukaryotes, and symmetrically cleaving enzymes found in Physarum polycephalum and in many bacteria...
  45. ncbi Evolution of RNA editing sites in the mitochondrial small subunit rRNA of the Myxomycota
    Uma Krishnan
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas, USA
    Methods Enzymol 424:197-220. 2007
    ..site location in the core region of the small subunit rRNA gene in the mtDNA of seven myxomycetes, including Physarum polycephalum. We find that the unique type of insertional RNA editing present in mitochondria of P...
  46. ncbi Evolution of hierarchical cytoplasmic inheritance in the plasmodial slime mold Physarum polycephalum
    Akiko Iwanaga
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812 8581, Japan
    Evolution 58:710-22. 2004
    ..for uniparental mitochondrial transmission is known between many mating types of plasmodial slime mold Physarum polycephalum...
  47. ncbi Structure, function and evolution of the mitochondrial division apparatus
    Tsuneyoshi Kuroiwa
    Laboratory of Cell Biology and Frontier Project Life s Adaptation Strategies of Environmental Change, Department of Life Sciences, College of Science, Rikkyo University, 3 34 1 Nishiikebukuro, Toshima ku, Tokyo 171 8501, Japan
    Biochim Biophys Acta 1763:510-21. 2006
    ..electron-dense MD ring in the cytoplasm at the constriction sites of dividing mitochondria in the slime mold Physarum polycephalum, and then two large MD rings (with outer cytoplasmic and inner matrix sides) in the red alga ..
  48. pmc Abundant 5S rRNA-like transcripts encoded by the mitochondrial genome in amoebozoa
    Charles E Bullerwell
    Department of Biochemistry and Molecular Biology, Room 8 F2, Sir Charles Tupper Medical Building, Dalhousie University, 5840 College Street, Halifax, Nova Scotia B3H 1X5, Canada
    Eukaryot Cell 9:762-73. 2010
    ..members of Amoebozoa, namely, in the lobose amoeba Hartmannella vermiformis and in the myxomycete slime mold Physarum polycephalum...
  49. ncbi Isolation and characterization of glutathione reductase from Physarum polycephalum and stage-specific expression of the enzyme in life-cycle stages with different oxidation-reduction levels
    Yoshiko Minami
    Department of Biochemistry, Faculty of Science, Okayama University of Science, 1 1 Ridai cho, Okayama 700 0005, Japan
    J Eukaryot Microbiol 50:317-23. 2003
    b>Physarum polycephalum has a life cycle with several distinct phases that have different oxidation-reduction requirements...
  50. ncbi Lens crystallins and their microbial homologs: structure, stability, and function
    R Jaenicke
    Institut fur Biophysik und Physikalische Biochemie, Universitat Regensburg, Germany
    Crit Rev Biochem Mol Biol 36:435-99. 2001
    ..Recent studies of natural and mutant vertebrate bg-crystallins as well as spherulin 3a from Physarum polycephalum and Protein S from Myxococcus xanthus allowed the correlation of structure and stability of ..
  51. ncbi Structure and properties of the recombinant NADH-cytochrome b5 reductase of Physarum polycephalum
    Terumi Ikegami
    Department of Biochemistry, Faculty of Science, Okayama University of Science, Okayama, Japan
    Biosci Biotechnol Biochem 71:783-90. 2007
    A cDNA for NADH-cytochrome b(5) reductase of Physarum polycephalum was cloned from a cDNA library, and the nucleotide sequence of the cDNA was determined (accession no. AB259870)...
  52. ncbi RNA editing is absent in a single mitochondrial gene of Didymium iridis
    Peter G Hendrickson
    Children s Memorial Research Center, Immunology Department, 2300 Children s Plaza, Mailstop 212, Chicago, Illinois 60614, USA
    Mycologia 102:1288-94. 2010
    ..The D. iridis sequence was compared to the putative nad3 gene in the related myxomycete Physarum polycephalum, which would require editing. Based on this comparison, editing sites could be predicted for the P...
  53. ncbi Sampling gene diversity across the supergroup Amoebozoa: large EST data sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp
    Russell F Watkins
    Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5
    Protist 159:269-81. 2008
    ..including two free-living amoebae (Acanthamoeba castellanii, Hartmannella vermiformis) and three slime molds (Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp...
  54. ncbi [A SC35-like protein is localized in the nucleus of Physarum polycephalum]
    Bo Hu
    Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China
    Yi Chuan Xue Bao 31:177-82. 2004
    After being labeled with an anti-SC35 antibody, the specimens of Physarum polycephalum at S, G2, prophase, metaphase and ana-telophase were observed with an Hitachi electron microscope and gold particles marking the location of the SC35-..
  55. pmc Structure of Physarum polycephalum cytochrome b5 reductase at 1.56 A resolution
    Sangwoo Kim
    Institute for Protein Research, Osaka University, 3 2 Yamada Oka, Suita, Osaka, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:274-9. 2007
    b>Physarum polycephalum cytochrome b(5) reductase catalyzes the reduction of cytochrome b(5) by NADH. The structure of P. polycephalum cytochrome b(5) reductase was determined at a resolution of 1.56 A...
  56. pmc RNA editing of 10 Didymium iridis mitochondrial genes and comparison with the homologous genes in Physarum polycephalum
    Stephen J Traphagen
    The English High School, Boston Public Schools, Boston, Massachusetts 02130, USA
    RNA 16:828-38. 2010
    ..of editing events and editing sequence features were very similar to those observed in the homologous genes of Physarum polycephalum, though the actual editing locations showed a variable degree of conservation...
  57. ncbi Physarum amoebae express a distinct fragmin-like actin-binding protein that controls in vitro phosphorylation of actin by the actin-fragmin kinase
    J Bailey
    Flanders Interuniversity Institute for Biotechnology VIB, Department of Medical Protein Research, Faculty of Medicine, University of Gent, Belgium
    Eur J Biochem 265:240-50. 1999
    Amoebae and plasmodia constitute the two vegetative growth phases of the Myxomycete Physarum. In vitro and in vivo phosphorylation of actin in plasmodia is tightly controlled by fragmin P, a plasmodium-specific actin-binding protein that ..
  58. ncbi Platelet-activating factor acetylhydrolases: broad substrate specificity and lipoprotein binding does not modulate the catalytic properties of the plasma enzyme
    J H Min
    Department of Biochemistry and Chemistry, University of Washington, Seattle, Washington 98195, USA
    Biochemistry 40:4539-49. 2001
    ..homologous PAF-AH which occurs intracellularly [PAF-AH(II)] as well as for the PAF-AH from the lower eukaryote Physarum polycephalum although pPAF-AH and PAF-AH(II) tolerate the removal of the sn-3 headgroup better than the PAF-AH ..
  59. ncbi Calcium-induced conformational changes in Leishmania infantum kinetoplastid membrane protein-11
    M A Fuertes
    Facultad de Ciencias, Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Contoblanco, Spain
    J Biol Inorg Chem 6:107-17. 2001
    ..the existence of 67% similarity and 33% identity with the EF-hand of the plasmodia-specific 40-kDa protein from Physarum polycephalum...
  60. ncbi Localization of the newly initiated and processed ribosomal primary transcripts during the mitotic cycle in Physarum polycephalum
    G Pierron
    Laboratoire Organisation Fonctionnelle du Noyau, UPR 9044, Institut de Recherches sur le Cancer, Villejuif, 94801, France
    Exp Cell Res 229:407-20. 1996
    ..prophasic disintegration of the nucleolus, during the "closed" mitosis of the naturally synchronous plasmodium of Physarum polycephalum...
  61. ncbi The complete DNA sequence of the mitochondrial genome of Physarum polycephalum
    H Takano
    Department of Biological Science, Faculty of Science, Kumamoto University, Japan
    Mol Gen Genet 264:539-45. 2001
    ..No guide RNA-like sequences were observed in the mtDNA of P. polycephalum. Comparison with reported Physarum mtDNA sequences suggested that sites of RNA editing vary among strains...
  62. ncbi Properties, intracellular localization, and stage-specific expression of membrane-bound beta-glucosidase, BglM1, from Physarum polycephalum
    Masato Hayase
    Department of Biochemistry, Okayama University of Science, 1 1 Ridai cho, Okayama 700 0005, Japan
    Int J Biochem Cell Biol 40:2141-50. 2008
    b>Physarum polycephalum expresses a membrane-bound beta-glucosidase (BglM1) with a molecular mass of 130 kDa...
  63. ncbi Maternal inheritance of mitochondria: multipolarity, multiallelism and hierarchical transmission of mitochondrial DNA in the true slime mold Physarum polycephalum
    Yohsuke Moriyama
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bldg FSB 601, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japane
    J Plant Res 123:139-48. 2010
    Direct evidence of digestion of paternal mitochondrial DNA (mtDNA) has been found in the true slime mold Physarum polycephalum...
  64. ncbi Clathrin is involved in organization of mitotic spindle and phragmoplast as well as in endocytosis in tobacco cell cultures
    H Tahara
    Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo, Japan
    Protoplasma 230:1-11. 2007
    ..in Lilium longiflorum germinating pollen using a monoclonal antibody raised against myosin II heavy chain from Physarum polycephalum. In the present study, the equivalent polypeptide was also found in cultured tobacco BY-2 cells...
  65. ncbi Nucleotide sequence of pig plasma gelsolin. Comparison of protein sequence with human gelsolin and other actin-severing proteins shows strong homologies and evidence for large internal repeats
    M Way
    MRC Laboratory of Molecular Biology, Cambridge, England
    J Mol Biol 203:1127-33. 1988
    ..the gelsolin sequences with chicken brush border villin, severin from Dictyostelium discoideum and fragmin from Physarum polycephalum shows a strong evolutionary relationship between all these proteins...
  66. pmc Identification of a nuclear localization motif in the serine/arginine protein kinase PSRPK of physarum polycephalum
    Shide Liu
    Shenzhen Key Laboratory of Microbial Genetic Engineering and College of Life Science, Shenzhen University, Shenzhen, PR China
    BMC Biochem 10:22. 2009
    ..But no nuclear localization signal (NLS) of SRPKs was found. We isolated an SRPK-like protein PSRPK (GenBank accession No. DQ140379) from Physarum polycephalum previously, and identified a NLS of PSRPK in this study.
  67. pmc Glom is a novel mitochondrial DNA packaging protein in Physarum polycephalum and causes intense chromatin condensation without suppressing DNA functions
    Narie Sasaki
    Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan
    Mol Biol Cell 14:4758-69. 2003
    ..inducing agglomeration of mitochondrial chromosome), from highly condensed mt-nucleoids of the true slime mold, Physarum polycephalum...
  68. pmc Non-DNA-templated addition of nucleotides to the 3' end of RNAs by the mitochondrial RNA polymerase of Physarum polycephalum
    Mara L Miller
    Department of Molecular and Cell Biology, The University of Texas at Dallas, Box 830688, Richardson, TX 75083 0688, USA
    Mol Cell Biol 28:5795-802. 2008
    ..In the myxomycete Physarum polycephalum, nucleotides that are not specified by the mitochondrial DNA templates are inserted into some RNAs, ..
  69. pmc Early activated replication origins within the cell cycle-regulated histone H4 genes in Physarum
    M Benard
    Laboratoire Organisation Fonctionnelle du Noyau, CNRS UPR 1983, IFR 1221, F 94801 Villejuif, France
    Nucleic Acids Res 27:2091-8. 1999
    ..H4 gene family, H4-1 and H4-2, are replicated at the onset of S phase in the naturally synchronous plasmodium of Physarum polycephalum, suggesting that they are flanked by replication origins...
  70. pmc Two forms of RNA editing are required for tRNA maturation in Physarum mitochondria
    Jonatha M Gott
    Center for RNA Molecular Biology, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    RNA 16:482-8. 2010
    The mitochondrial genome of Physarum polycephalum encodes five tRNAs, four of which are edited by nucleotide insertion...
  71. pmc A novel type of protein kinase phosphorylates actin in the actin-fragmin complex
    L Eichinger
    Adolf Butenandt Institut Zellbiologie, Ludwig Maximilians Universitat, Munchen, Germany
    EMBO J 15:5547-56. 1996
    Actin-fragmin kinase (AFK) from Physarum polycephalum specifically phosphorylates actin in the EGTA-resistant 1:1 actin-fragmin complex...
  72. ncbi Invalidation of Hyperamoeba by transferring its species to other genera of Myxogastria
    Anna Maria Fiore-Donno
    Zoology Department, University of Oxford, South Parks Road, OX13PS Oxford, United Kingdom
    J Eukaryot Microbiol 57:189-96. 2010
    ..Phylogenetic analyses show unambiguously that the type species Hyperamoeba flagellata is a Physarum (Physarum flagellatum comb. nov...
  73. ncbi Regulation of levels of actin threonine phosphorylation during life cycle of Physarum polycephalum
    Yuki Shirai
    Department of Biology, Ochanomizu University, Bunkyo ku, Tokyo 112 8610, Japan
    Cell Motil Cytoskeleton 63:77-87. 2006
    Under various environmental stresses, the true slime mold Physarum polycephalum converts into dormant forms, such as microcysts, sclerotia, and spores, which can survive in adverse environments for a considerable period of time...
  74. pmc The crystal structure of the Physarum polycephalum actin-fragmin kinase: an atypical protein kinase with a specialized substrate-binding domain
    S Steinbacher
    Abteilung Strukturforschung, Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
    EMBO J 18:2923-9. 1999
    ..In plasmodia of Physarum polycephalum, the F-actin capping activity of the actin-fragmin complex is regulated by the phosphorylation of ..
  75. ncbi G1-phase arrest is not a prerequisite for encystment in Physarum
    R W Anderson
    Department of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom
    Exp Cell Res 236:351-4. 1997
    Amoebae of the Myxomycete Physarum polycephalum form resistant, walled cysts when the food bacteria in a culture have been consumed. No G1 phase has been detected in the vegetative amoebal cell cycle, most of which comprises the G2 phase...
  76. ncbi Spatiotemporal symmetry in rings of coupled biological oscillators of Physarum plasmodial slime mold
    A Takamatsu
    PRESTO, Japan Science and Technology Corporation, Saitama, Japan
    Phys Rev Lett 87:078102. 2001
    Spatiotemporal patterns in rings of coupled biological oscillators of the plasmodial slime mold, Physarum polycephalum, were investigated by comparing with results analyzed by the symmetric Hopf bifurcation theory based on group theory...
  77. ncbi Chromatin remodeling by cell cycle stage-specific extracts from Physarum polycephalum
    C Thiriet
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, NY USA
    Eur J Cell Biol 78:214-20. 1999
    ..The slime mold Physarum polycephalum presents the attractive advantage of natural synchrony of the cell cycle in several million nuclei...
  78. ncbi RNase-dependent discontinuities associated with the crossovers of spontaneously formed joint DNA molecules in Physarum polycephalum
    Chrystelle Maric
    Laboratoire Réplication de l ADN et Ultrastructure du Noyau, CNRS FRE 3238, Institut Andre Lwoff, 7 rue Guy Moquet, BP 8, 94801, Villejuif Cedex, France
    Chromosoma 119:601-11. 2010
    ..Here, we studied their structure and frequency of formation in Physarum polycephalum. By "3D gels", we evidenced that they are not made of four continuous DNA strands...
  79. ncbi Physarum polymalic acid hydrolase: Recombinant expression and enzyme activation
    Wolfgang Mueller
    Biophysik und Physikalische Biochemie, Universitaet Regensburg, 93040 Regensburg, Germany
    Biochem Biophys Res Commun 377:735-40. 2008
    ..acid together with its tailoring specific exohydrolase is purified from plasmodium cultures of the slime mold Physarum polycephalum, a member of the phylum myxomycota...
  80. pmc RNA B is the major nucleolar trimethylguanosine-capped small nuclear RNA associated with fibrillarin and pre-rRNAs in Trypanosoma brucei
    T Hartshorne
    Intercampus Program in Molecular Parasitology, School of Pharmacy, University of California, San Francisco 94143 1204
    Mol Cell Biol 13:144-54. 1993
    ..A 35-kDa T. brucei fibrillarin homolog was identified by using an anti-Physarum fibrillarin monoclonal antibody...
  81. ncbi Dry stress-induced phosphorylation of Physarum actin
    K Furuhashi
    Department of Biology, Faculty of Education, Shiga University, Japan
    Biochem Biophys Res Commun 242:653-8. 1998
    Protein phosphorylation plays important roles in a variety of stress responses. Although plasmodium of Physarum polycephalum rapidly grows and shows an active cytoplasmic streaming under nutrient and wet conditions, dry stress transforms ..
  82. ncbi Evolution of a protein superfamily: relationships between vertebrate lens crystallins and microorganism dormancy proteins
    G Wistow
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20982
    J Mol Evol 30:140-5. 1990
    A search of sequence databases shows that spherulin 3a, an encystment-specific protein of Physarum polycephalum, is probably structurally related to the beta- and gamma-crystallins, vertebrate ocular lens proteins, and to Protein S, a ..
  83. pmc Linker histone phosphorylation regulates global timing of replication origin firing
    Christophe Thiriet
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Biol Chem 284:2823-9. 2009
    ..Here, we show that linker histone (H1) is involved in the global regulation of DNA replication in Physarum polycephalum...
  84. ncbi Generation of highly site-specific DNA double-strand breaks in human cells by the homing endonucleases I-PpoI and I-CreI
    R J Monnat
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    Biochem Biophys Res Commun 255:88-93. 1999
    ..have determined the ability of two well-characterized eukaryotic homing endonucleases, I-PpoI from the myxomycete Physarum polycephalum and I-CreI from the green alga Chlamydomonas reinhardtii, to generate site-specific DNA double-..
  85. pmc Amoeboid organism solves complex nutritional challenges
    Audrey Dussutour
    Centre de Recherches sur la Cognition Animale, Universite Paul Sabatier, 31062 Toulouse, France
    Proc Natl Acad Sci U S A 107:4607-11. 2010
    ..organisms coordinate the search for multiple nutrients? We address this question in the acellular slime mold Physarum polycephalum and show that this extraordinary organism can make complex nutritional decisions, despite lacking a ..
  86. ncbi Biological functions of a novel lipid mediator, cyclic phosphatidic acid
    Kimiko Murakami-Murofushi
    Department of Biology, Faculty of Science, Ochanomizu University, 2 1 1 Ohtsuka, Bunkyo ku, 112 8610, Tokyo, Japan
    Biochim Biophys Acta 1582:1-7. 2002
    ..bioactive lipid, cyclic phosphatidic acid (cPA), was isolated originally from myxoamoebae of a true slime mold, Physarum polycephalum, and has now been detected in a wide range of organisms from slime molds to humans...
  87. pmc A first glimpse at the transcriptome of Physarum polycephalum
    Gernot Glockner
    Leibniz Institute for Age Research Fritz Lipmann Institute, Beutenbergstr, 11, D 07745 Jena, Germany
    BMC Genomics 9:6. 2008
    b>Physarum polycephalum, an acellular plasmodial species belongs to the amoebozoa, a major branch in eukaryote evolution...
  88. ncbi [Transient expression in microplasmodia of Physarum polycephalum]
    Shide Liu
    Shenzhen Key Laboratory of Microbial and Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China
    Sheng Wu Gong Cheng Xue Bao 25:854-62. 2009
    The plasmodium of Physarum polycephalum is a suitable eukaryotic cell for cell cycle investigation, but there is no compatible transient expression system for the plasmodium...
  89. pmc Transcriptomic changes arising during light-induced sporulation in Physarum polycephalum
    Israel Barrantes
    Max Planck Institute for Dynamics of Complex Technical Systems and Magdeburg Centre for Systems Biology MaCS, Otto von Guericke University, Magdeburg, Germany
    BMC Genomics 11:115. 2010
    b>Physarum polycephalum is a free-living amoebozoan protist displaying a complex life cycle, including alternation between single- and multinucleate stages through sporulation, a simple form of cell differentiation...
  90. ncbi A novel Physarum polycephalum SR protein kinase specifically phosphorylates the RS domain of the human SR protein, ASF/SF2
    Shide Liu
    Shenzhen Key Laboratory of Microbial and Genetic Engineering, College of Life Science Shenzhen University, Shenzhen, China
    Acta Biochim Biophys Sin (Shanghai) 41:657-67. 2009
    A 1591-bp cDNA of a serine-rich protein kinase (SRPK)-like protein has been identified in Physarum polycephalum (GenBank accession No. DQ140379). The cDNA contains two repeat sequences at bp 1-153 and bp 395-547...
  91. pmc Physarum nitric oxide synthases: genomic structures and enzymology of recombinant proteins
    Simon Messner
    Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3 VI, Innsbruck, Austria
    Biochem J 418:691-700. 2009
    b>Physarum polycephalum expresses two closely related, calcium-independent NOSs (nitric oxide synthases). In our previous work, we showed that both NOSs are induced during starvation and apparently play a functional role in sporulation...
  92. pmc The one-kilobase DNA fragment upstream of the ardC actin gene of Physarum polycephalum is both a replicator and a promoter
    G Pierron
    Laboratoire Organisation Fonctionnelle du Noyau, UPR 9044, CNRS, Institut de Recherches sur le Cancer, 94801 Villejuif, France
    Mol Cell Biol 19:3506-14. 1999
    The 1-kb DNA fragment upstream of the ardC actin gene of Physarum polycephalum promotes the transcription of a reporter gene either in a transient-plasmid assay or as an integrated copy in an ectopic position, defining this region as the ..
  93. ncbi A calmodulin-dependent protein kinase from lower eukaryote Physarum polycephalum
    Akio Nakamura
    Department of Molecular and Cellular Pharmacology, Faculty of Medicine, Gunma University Graduate School of Medicine, Gunma 371 8511, Japan
    Biochem Biophys Res Commun 328:838-44. 2005
    A full-length cDNA coding a calmodulin (CaM)-dependent protein kinase gene was cloned from Physarum plasmodia poly(A)-RNA by polymerase chain reaction with the oligonucleotide primers that were designed after the amino acid sequence of ..
  94. ncbi Characteristics of pattern formation and evolution in approximations of Physarum transport networks
    Jeff Jones
    Centre for Unconventional Computing, University of the West of England, Bristol, BS16 1QY, UK
    Artif Life 16:127-53. 2010
    ..Inspired by one such organism, the true slime mold Physarum polycephalum, we present examples of complex emergent pattern formation and evolution formed by a population of ..
  95. pmc Distinct roles for sequences upstream of and downstream from Physarum editing sites
    Amy C Rhee
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    RNA 15:1753-65. 2009
    RNAs in the mitochondria of Physarum polycephalum contain nonencoded nucleotides that are added during RNA synthesis...
  96. ncbi Action spectrum for sporulation and photoavoidance in the plasmodium of Physarum polycephalum, as modified differentially by temperature and starvation
    T Nakagaki
    Graduate School of Human Informatics, Nagoya University, Japan
    Photochem Photobiol 64:859-62. 1996
    The plasmodium of the myxomycete Physarum polycephalum sporulates in bright natural environments, suggesting a relationship between photobehavior and sporulation...
  97. ncbi Purification and partial characterization of a nucleolar scleroderma antigen (Mr = 34,000; pI, 8.5) rich in NG,NG-dimethylarginine
    M A Lischwe
    J Biol Chem 260:14304-10. 1985
    ..In the first 31 residues, there are 16 glycine, 6 DMA, and 3 phenylalanine residues. This is a novel demonstration of clusters of glycine and DMA in a protein...
  98. pmc Polyamines in microorganisms
    C W Tabor
    Microbiol Rev 49:81-99. 1985
  99. doi Cellular memory hints at the origins of intelligence
    Philip Ball
    Nature 451:385. 2008
  100. ncbi Determination of absolute stereochemistry, total synthesis, and evaluation of peptides from the myxomycete Physarum melleum
    Shuwa Hanazawa
    Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 263 8522, Japan
    Bioorg Med Chem Lett 18:95-8. 2008
    The absolute stereochemistry of melleumin A (1) and B (2), novel peptide compounds isolated from the myxomycete Physarum melleum, was determined by synthesis of their segments and by a modified Mosher's method...
  101. ncbi [Isolation and characterization of the ALP1 protease from Aspergillus fumigatus and its protein inhibitor from Physarium polycephalum]
    D A Davies
    Bioorg Khim 31:259-68. 2005
    ..A thermostable protein inhibitor of the ALP1 protease was isolated from the plasmodium of the myxomycete Physarum polycephalum. Its molecular mass is 32-33 kDa. The inhibitor inhibits the ALP1 protease activity with IC50 of 0...

Research Grants19

  1. Nanoconjugate based on polymalic acid for brain tumor treatment
    Julia Y Ljubimova; Fiscal Year: 2013
    ..system (Polycefin) based on naturally derived nanoplatform, poly(p-L-malic acid) (PMLA) fi-om a slime mold, Physarum polycephalum...
  2. REGULATION OF ORNITHINE DECARBOXYLASE ACTIVITY
    John Mitchell; Fiscal Year: 1980
    ..of the regulation of this enzyme will be performed on the readily available enzyme from the primitive eukaryote, Physarum polycephalum, while a similar investigation, initially in less detail, will be made of the two enzyme forms in ..
  3. ULTRASTRUCTURAL STUDIES OF GENETIC ACTIVITY
    Oscar Miller; Fiscal Year: 1992
    ..interactions will be studied in eukaryotes using synchronously replicating yeast cells, Drosophila embryos and Physarum cultures...
  4. PHYSICAL STUDIES OF CONFORMATIONAL CHANGE IN CHROMATIN
    RODNEY HARRINGTON; Fiscal Year: 1990
    ..to characterize the structure and conformation of chromatin from the naturally mitotically synchronous slime mold Physarum polycephalum...
  5. RIBOSOMAL DNA IN PHYSARUM POLYCEPHALUM
    VOLKER VOGT; Fiscal Year: 1990
    The gene coding for ribosomal RNA in Physarum polycephalum exist as a collection of about 200 extrachromosomal DNA molecules 60 kb in size. This rDNA can be purified readily; most of it has been cloned and sequenced...
  6. MITOCHONDRIAL GENE EXPRESSION IN PHYSARUM POLYCEPHALUM
    Dennis Miller; Fiscal Year: 1992
    ..A unique step in mitochondrial gene expression has been identified in the mitochondria of the myxomycot, Physarum polycephalum...
  7. THE MECHANISM OF RADIATION-INDUCED MITOTIC DELAY
    NANCY OLEINICK; Fiscal Year: 1980
    ..We are taking advantage of the natural synchrony of nuclear division in the plasmodial phase of Physarum polycephalum, in which we previously determined the times in late G2-early prophase when the plasmodium loses ..
  8. Transcription and Insertional editing in Physarum
    Jonatha Gott; Fiscal Year: 2006
    ..The long-term goal of this project is the elucidation of the mechanism of insertional RNA editing in Physarum mitochondria...
  9. MEMBRANE STRUCTURE, CELL CONTACT AND CELL FUSION
    Henry Aldrich; Fiscal Year: 1980
    Myxamoebae of Didymium iridis, Physarum polycephalum, Physarum flavicomum, and Didtypostelium discoideum will be studied to determine the relation of cell surface and membrane structure to sexual fusion processes...
  10. Tumor drug delivery systems based on polymalic acid
    Julia Y Ljubimova; Fiscal Year: 2010
    ..A new nanoscale drug delivery system (drug size 20-30 nm) based on naturally derived poly malic acid (PMLA) from Physarum polycephalum will be studied...
  11. NOVEL HUMAN GENE SPECIFIC REAGENTS FROM HOMING ENDOS
    Raymond Monnat; Fiscal Year: 2005
    ..The availability of human GSR's may also allow modification of altered human genes responsible for specific diseases or genes of pathogenic microorganisms including bacteria and viruses, parasites, etc. ..