tetrahymena thermophila

Summary

Summary: A species of ciliate protozoa used in genetic and cytological research.

Top Publications

  1. pmc Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote
    Jonathan A Eisen
    The Institute for Genomic Research, Rockville, Maryland, United States of America
    PLoS Biol 4:e286. 2006
  2. pmc Shiga toxin as a bacterial defense against a eukaryotic predator, Tetrahymena thermophila
    William Lainhart
    Department of Biological Sciences, University at Buffalo, Cooke Hall, North Campus, Buffalo, NY 14260, USA
    J Bacteriol 191:5116-22. 2009
  3. pmc Tetrahymena thermophila, a unicellular eukaryote with separate germline and somatic genomes
    Eduardo Orias
    Department of Molecular, Cellular and Developmental Biology, University of California at Santa Barbara, Santa Barbara, CA 93106, USA
    Res Microbiol 162:578-86. 2011
  4. pmc Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closure
    Robert S Coyne
    J Craig Venter Institute formerly The Institute for Genomic Research, 9704 Medical Center Dr, Rockville, MD, USA
    BMC Genomics 9:562. 2008
  5. pmc The two domains of centrin have distinct basal body functions in Tetrahymena
    Tyson Vonderfecht
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, CO 80309, USA
    Mol Biol Cell 22:2221-34. 2011
  6. pmc Gene network landscape of the ciliate Tetrahymena thermophila
    Jie Xiong
    Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China
    PLoS ONE 6:e20124. 2011
  7. ncbi Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation
    J E Brownell
    Department of Biology, University of Rochester, New York, 14627, USA
    Cell 84:843-51. 1996
  8. pmc Transcriptome analysis of the model protozoan, Tetrahymena thermophila, using Deep RNA sequencing
    Jie Xiong
    Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People s Republic of China
    PLoS ONE 7:e30630. 2012
  9. pmc A domesticated piggyBac transposase plays key roles in heterochromatin dynamics and DNA cleavage during programmed DNA deletion in Tetrahymena thermophila
    Chao Yin Cheng
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
    Mol Biol Cell 21:1753-62. 2010
  10. ncbi Organization and pairing of meiotic chromosomes in the ciliate Tetrahymena thermophila
    Josef Loidl
    Institute of Botany, University of Vienna, Rennweg 14, 1030 Vienna, Austria
    J Cell Sci 117:5791-801. 2004

Detail Information

Publications236 found, 100 shown here

  1. pmc Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote
    Jonathan A Eisen
    The Institute for Genomic Research, Rockville, Maryland, United States of America
    PLoS Biol 4:e286. 2006
    The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct nuclei within a single cell...
  2. pmc Shiga toxin as a bacterial defense against a eukaryotic predator, Tetrahymena thermophila
    William Lainhart
    Department of Biological Sciences, University at Buffalo, Cooke Hall, North Campus, Buffalo, NY 14260, USA
    J Bacteriol 191:5116-22. 2009
    ..We show here that Tetrahymena thermophila, a bacterivorous predator, is killed when cocultured with bacteria bearing a Shiga toxin (Stx)-encoding ..
  3. pmc Tetrahymena thermophila, a unicellular eukaryote with separate germline and somatic genomes
    Eduardo Orias
    Department of Molecular, Cellular and Developmental Biology, University of California at Santa Barbara, Santa Barbara, CA 93106, USA
    Res Microbiol 162:578-86. 2011
    b>Tetrahymena thermophila is a ciliate--a unicellular eukaryote. Remarkably, every cell maintains differentiated germline and somatic genomes: one silent, the other expressed...
  4. pmc Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closure
    Robert S Coyne
    J Craig Venter Institute formerly The Institute for Genomic Research, 9704 Medical Center Dr, Rockville, MD, USA
    BMC Genomics 9:562. 2008
    b>Tetrahymena thermophila, a widely studied model for cellular and molecular biology, is a binucleated single-celled organism with a germline micronucleus (MIC) and somatic macronucleus (MAC)...
  5. pmc The two domains of centrin have distinct basal body functions in Tetrahymena
    Tyson Vonderfecht
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, CO 80309, USA
    Mol Biol Cell 22:2221-34. 2011
    ..The results also show that the two domains of Cen1 are able to bind Ca(2+) and that perturbation of Ca(2+) binding affects Cen1 function. In all, the data suggest that the two domains of Cen1 have distinct functions...
  6. pmc Gene network landscape of the ciliate Tetrahymena thermophila
    Jie Xiong
    Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China
    PLoS ONE 6:e20124. 2011
    ..In this study, we used different methods to find gene networks in the ciliate Tetrahymena thermophila, and describe some important properties of this network, such as modules and hubs.
  7. ncbi Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation
    J E Brownell
    Department of Biology, University of Rochester, New York, 14627, USA
    Cell 84:843-51. 1996
    ..These findings shed light on the biochemical function of the evolutionarily conserved Gcn5p-Ada complex, directly linking histone acetylation to gene activation, and indicate that histone acetylation is a targeted phenomenon...
  8. pmc Transcriptome analysis of the model protozoan, Tetrahymena thermophila, using Deep RNA sequencing
    Jie Xiong
    Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People s Republic of China
    PLoS ONE 7:e30630. 2012
    The ciliated protozoan Tetrahymena thermophila is a well-studied single-celled eukaryote model organism for cellular and molecular biology. However, the lack of extensive T...
  9. pmc A domesticated piggyBac transposase plays key roles in heterochromatin dynamics and DNA cleavage during programmed DNA deletion in Tetrahymena thermophila
    Chao Yin Cheng
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
    Mol Biol Cell 21:1753-62. 2010
    ..These findings suggest that Tpb2p plays a key role in the assembly of specialized DNA elimination chromatin architectures and is likely responsible for the DNA cleavage step of programmed DNA deletion...
  10. ncbi Organization and pairing of meiotic chromosomes in the ciliate Tetrahymena thermophila
    Josef Loidl
    Institute of Botany, University of Vienna, Rennweg 14, 1030 Vienna, Austria
    J Cell Sci 117:5791-801. 2004
    During meiotic prophase in the ciliate Tetrahymena thermophila micronuclei dramatically elongate and form thread-like crescents...
  11. pmc Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophila
    Mary T Couvillion
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Genes Dev 23:2016-32. 2009
    ..The ciliate Tetrahymena thermophila encodes numerous PPD proteins exclusively of the Piwi clade...
  12. pmc Predation on multiple trophic levels shapes the evolution of pathogen virulence
    Ville Petri Friman
    Department of Biological and Environmental Science, Centre of Excellence in Evolutionary Research, University of Jyvaskyla, Jyvaskyla, Finland
    PLoS ONE 4:e6761. 2009
    ..Exposing opportunistic bacterial pathogen Serratia marcescens to predation by protozoan Tetrahymena thermophila decreased its virulence when measured as host moth Parasemia plantaginis survival...
  13. pmc A developmentally regulated gene, ASI2, is required for endocycling in the macronuclear anlagen of Tetrahymena
    Lihui Yin
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53201 1881, USA
    Eukaryot Cell 9:1343-53. 2010
    ..Thus, endocycling is conserved from ciliates to multicellular organisms. The gene ASI2 in Tetrahymena thermophila encodes a putative signal transduction receptor...
  14. pmc 1+1 = 3: a fusion of 2 enzymes in the methionine salvage pathway of Tetrahymena thermophila creates a trifunctional enzyme that catalyzes 3 steps in the pathway
    Hannah M W Salim
    Biology Department, Pomona College, Claremont, California, USA
    PLoS Genet 5:e1000701. 2009
    ..a computational approach to identify the enzymes of the methionine salvage pathway in another eukaryote, Tetrahymena thermophila. In this organism, the pathway has two fused genes, MTNAK and MTNBD...
  15. ncbi Ecotoxicity of nanoparticles of CuO and ZnO in natural water
    I Blinova
    Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn 12618, Estonia
    Environ Pollut 158:41-7. 2010
    ..AFW) and in natural waters to crustaceans Daphnia magna and Thamnocephalus platyurus and protozoan Tetrahymena thermophila was compared...
  16. pmc The Tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus
    Tomoko Noto
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3, A 1030 Vienna, Austria
    Cell 140:692-703. 2010
    ..These results suggest that Giw1p senses the state of Twi1p-associated siRNAs and selectively transports the mature Twi1p-siRNA complex into the nucleus...
  17. pmc Template boundary definition in mammalian telomerase
    Jiunn Liang Chen
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Genes Dev 17:2747-52. 2003
    ..The divergent structure of boundary definition elements in mammals, yeast, and ciliates suggests diverse mechanisms for template boundary definition in telomerase...
  18. ncbi Pulsed-resource dynamics constrain the evolution of predator-prey interactions
    Ville Petri Friman
    Centre of Excellence in Evolutionary Research, Department of Biological and Environmental Science, University of Jyvaskyla, Finland
    Am Nat 177:334-45. 2011
    ..and ecological dynamics between the prey bacterium Serratia marcescens and the predatory protozoan Tetrahymena thermophila. Predation increased the frequency of defensive, nonpigmented prey types, which bore competitive costs in ..
  19. ncbi Exploring the mitochondrial proteome of the ciliate protozoon Tetrahymena thermophila: direct analysis by tandem mass spectrometry
    Daryl G S Smith
    Department of Chemistry and Centre for Research in Mass Spectrometry, York University, Toronto, Ontario, Canada
    J Mol Biol 374:837-63. 2007
    ..Highly purified mitochondria from Tetrahymena thermophila, a ciliated protozoon, were digested exhaustively with trypsin and the resulting peptides subjected to ..
  20. pmc Detection of fused genes in eukaryotic genomes using gene deFuser: analysis of the Tetrahymena thermophila genome
    Hannah M W Salim
    Department of Biology, Pomona College, Claremont, California 91711, USA
    BMC Bioinformatics 12:279. 2011
    ..We have developed a program, Gene deFuser, that analyzes uploaded protein sequence files for characteristics of gene fusion events and presents the results in a convenient web interface...
  21. ncbi Whole genome studies of Tetrahymena
    Robert S Coyne
    Department of Genomic Medicine, J Craig Venter Institute, Rockville, MD, USA
    Methods Cell Biol 109:53-81. 2012
    ..decade, genomic studies have emerged as essential and highly productive tools to explore the biology of Tetrahymena thermophila. The current major resources, which have been extensively mined by the research community, are the ..
  22. ncbi Marked amplification and diversification of products of ras genes from rat brain, Rab GTPases, in the ciliates Tetrahymena thermophila and Paramecium tetraurelia
    Yumiko Saito-Nakano
    Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan
    J Eukaryot Microbiol 57:389-99. 2010
    ..Rab encoded in the genome correlates with multicellularity; however, we found that unicellular ciliates Tetrahymena thermophila (Tt) and Paramecium tetraurelia (Pt) possess many more Rab genes in their genome than the 64 HsRab genes ..
  23. pmc Gigantic macroautophagy in programmed nuclear death of Tetrahymena thermophila
    Takahiko Akematsu
    Division of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Ishikawa, JP
    Autophagy 6:901-11. 2010
    ....
  24. pmc Role of apoptosis-inducing factor (AIF) in programmed nuclear death during conjugation in Tetrahymena thermophila
    Takahiko Akematsu
    Division of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Shizenken, Kakuma machi, Kanazawa, Ishikawa, Japan
    BMC Cell Biol 11:13. 2010
    ..In Tetrahymena thermophila, when the new macronucleus differentiates, the parental macronucleus is selectively eliminated from the ..
  25. pmc An RPA-related sequence-specific DNA-binding subunit of telomerase holoenzyme is required for elongation processivity and telomere maintenance
    Bosun Min
    University of California, Berkeley, 94720 3200, USA
    Mol Cell 36:609-19. 2009
    ..Here, we present the identification of three additional subunits of Tetrahymena thermophila telomerase holoenzyme...
  26. ncbi In vitro splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence
    T R Cech
    Department of Chemistry, University of Colorado, Boulder 80309
    Cell 27:487-96. 1981
    ..According to this model, the guanosine cofactor provides the free 3' hydroxyl necessary to initiate a series of three transfers that results in splicing of the pre-rRNA and cyclization of the excised IVS...
  27. ncbi Robust method for proteome analysis by MS/MS using an entire translated genome: demonstration on the ciliome of Tetrahymena thermophila
    Jeffrey C Smith
    Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3
    J Proteome Res 4:909-19. 2005
    ..demonstrated with the identification of 223 cilia proteins in the unicellular eukaryotic model organism Tetrahymena thermophila, whose initial genomic sequence draft was released in November 2003...
  28. pmc Complete sequence of the mitochondrial genome of Tetrahymena thermophila and comparative methods for identifying highly divergent genes
    Clifford F Brunk
    Department of Organismic Biology, Ecology and Evolution, University of California Los Angeles, Los Angeles, CA 90095 1606, USA
    Nucleic Acids Res 31:1673-82. 2003
    The complete sequence of the mitochondrial genome of Tetrahymena thermophila has been determined and compared with the mitochondrial genome of Tetrahymena pyriformis. The sequence similarity clearly indicates homology of the entire T...
  29. ncbi Tetrahymena thermophila
    Kathleen Collins
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Curr Biol 15:R317-8. 2005
  30. ncbi Toxicity of ZnO and CuO nanoparticles to ciliated protozoa Tetrahymena thermophila
    Monika Mortimer
    Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia
    Toxicology 269:182-9. 2010
    The toxic effects of nanoparticles (NPs) of ZnO and CuO to particle-ingesting model organism protozoa Tetrahymena thermophila were evaluated...
  31. ncbi Both carboxy-terminal tails of alpha- and beta-tubulin are essential, but either one will suffice
    Jianming Duan
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Curr Biol 12:313-6. 2002
    ..Using mutagenesis and homologous gene replacement in Tetrahymena thermophila, we analyzed mutations, deletions, tail switches, and tail duplications of alpha- and beta-tubulin CTTs...
  32. pmc Dynein-2 affects the regulation of ciliary length but is not required for ciliogenesis in Tetrahymena thermophila
    Vidyalakshmi Rajagopalan
    Department of Biology, Harvey Mudd College, Claremont, CA 91711, USA
    Mol Biol Cell 20:708-20. 2009
    ..These results demonstrate that dynein-2 contributes to the regulation of ciliary length but is not required for ciliogenesis in Tetrahymena...
  33. pmc Two GW repeat proteins interact with Tetrahymena thermophila argonaute and promote genome rearrangement
    Janna Bednenko
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Mol Cell Biol 29:5020-30. 2009
    In conjugating Tetrahymena thermophila, massive DNA elimination occurs upon the development of the new somatic genome from the germ line genome...
  34. pmc Biological and biochemical functions of RNA in the tetrahymena telomerase holoenzyme
    Doreen D Cunningham
    Department of Molecular and Cell Biology, 16 Barker Hall, University of California, Berkeley, CA 94720 3204, USA
    Mol Cell Biol 25:4442-54. 2005
    ..These findings demonstrate nontemplate functions of TER that are critical for the telomerase holoenzyme catalytic cycle and for proper telomere length maintenance in vivo...
  35. pmc Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65
    Mahavir Singh
    Department of Chemistry, and the Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095 1569, USA
    Mol Cell 47:16-26. 2012
    ..Additionally, our studies define a structurally homologous domain (xRRM) in genuine La and LARP7 proteins and suggest a general mode of RNA binding for biogenesis of their diverse RNA targets...
  36. ncbi Mapping of DNA binding sites in the Tetrahymena telomerase holoenzyme proteins by UV cross-linking and mass spectrometry
    Karin Kertesz Rosenfeld
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    J Mol Biol 410:77-92. 2011
    ..Our study constitutes the first direct mapping of DNA interaction sites in telomerase holoenzyme complexes. This mapping represents a significant contribution to the understanding of the mechanism of telomere extension by telomerase...
  37. pmc A novel RNA binding domain in tetrahymena telomerase p65 initiates hierarchical assembly of telomerase holoenzyme
    Catherine M O'Connor
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3204, USA
    Mol Cell Biol 26:2029-36. 2006
    ..These findings elucidate an extensive network of p65-TER recognition specificity and define a novel p65 RNA binding domain that initiates telomerase holoenyzme biogenesis...
  38. pmc Roles of telomerase reverse transcriptase N-terminal domain in assembly and activity of Tetrahymena telomerase holoenzyme
    Barbara Eckert
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200, USA
    J Biol Chem 287:12805-14. 2012
    ..Our results add to the understanding of telomerase holoenzyme architecture and TERT domain functions with direct implications for the unique mechanism of single-stranded repeat synthesis...
  39. pmc Mass spectrometric imaging of highly curved membranes during Tetrahymena mating
    Sara G Ostrowski
    Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA
    Science 305:71-3. 2004
    ..This evidence suggests that biological fusion involves and might in fact be driven by a heterogeneous redistribution of lipids at the fusion site...
  40. pmc Mapping three classical isozyme loci in tetrahymena: meiotic linkage of EstA to the ChxA linkage group
    S L Allen
    Department of Biology, University of Michigan, Ann Arbor 48109 1048, USA
    Genetics 144:1489-96. 1996
    ..method for mapping conventional loci and obtaining meiotic linkage data for the ciliated protozoan Tetrahymena thermophila. By coupling nullisomic deletion mapping with meiotic linkage mapping, loci known to be located on a ..
  41. ncbi Flow cytometry assessment of cytotoxicity and reactive oxygen species generation by single and binary mixtures of cadmium, zinc and copper on populations of the ciliated protozoan Tetrahymena thermophila
    Andrea Gallego
    Departamento de Microbiologia III, Facultad de Biologia, Cl Jose Antonio Novais 2, Universidad Complutense, 28040 Madrid, Spain
    Chemosphere 68:647-61. 2007
    ..cytometry, the cytotoxicity of the heavy metals Cd, Zn and Cu on populations of the ciliated protozoan Tetrahymena thermophila. The obtained LC(50) for these metals was estimated as 0.195mgCdl(-1), 3.58mgZnl(-1) and 0...
  42. pmc Microarray analyses of gene expression during the Tetrahymena thermophila life cycle
    Wei Miao
    Department of Biology, University of Rochester, Rochester, New York, USA
    PLoS ONE 4:e4429. 2009
    The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression.
  43. pmc The condensin complex is essential for amitotic segregation of bulk chromosomes, but not nucleoli, in the ciliate Tetrahymena thermophila
    Marcella D Cervantes
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Cell Biol 26:4690-700. 2006
    The macronucleus of the binucleate ciliate Tetrahymena thermophila contains fragmented and amplified chromosomes that do not have centromeres, eliminating the possibility of mitotic nuclear division...
  44. pmc DYF-1 Is required for assembly of the axoneme in Tetrahymena thermophila
    Drashti Dave
    Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Eukaryot Cell 8:1397-406. 2009
    ..Our observations support the conserved function for DYF-1 in axoneme assembly or stability but also show that the consequences of loss of DYF-1 for axoneme segments are organism specific...
  45. pmc Product analysis illuminates the final steps of IES deletion in Tetrahymena thermophila
    S V Saveliev
    Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706-1544, USA
    EMBO J 20:3251-61. 2001
    DNA sequences (IES elements) eliminated from the developing macronucleus in the ciliate Tetrahymena thermophila are released as linear fragments, which have now been detected and isolated...
  46. ncbi Exposure to CuO nanoparticles changes the fatty acid composition of protozoa Tetrahymena thermophila
    Monika Mortimer
    Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia
    Environ Sci Technol 45:6617-24. 2011
    In the current study, the toxicity mechanism of nanosized CuO (nCuO) to the freshwater ciliated protozoa Tetrahymena thermophila was studied...
  47. ncbi The role of cortical geometry in the nuclear development of Tetrahymena thermophila
    J Gaertig
    Cellular Biology, University of Georgia, Athens 30602, USA
    J Eukaryot Microbiol 47:590-6. 2000
    ....
  48. pmc Experimental identification and analysis of macronuclear non-coding RNAs from the ciliate Tetrahymena thermophila
    Kasper L Andersen
    Department of Cellular and Molecular Medicine and Center for Non coding RNA in Technology and Health, The Panum Institute, University of Copenhagen, 3 Blegdamsvej, DK 2200N, Denmark
    Nucleic Acids Res 40:1267-81. 2012
    The ciliate Tetrahymena thermophila is an important eukaryotic model organism that has been used in pioneering studies of general phenomena, such as ribozymes, telomeres, chromatin structure and genome reorganization...
  49. pmc Expression, secretion and surface display of a human alkaline phosphatase by the ciliate Tetrahymena thermophila
    Ingo Aldag
    Cilian AG, Johann Krane Weg 42, Munster, Germany
    BMC Biotechnol 11:11. 2011
    b>Tetrahymena thermophila possesses many attributes that render it an attractive host for the expression of recombinant proteins...
  50. pmc Distinct functional roles of β-tubulin isotypes in microtubule arrays of Tetrahymena thermophila, a model single-celled organism
    Sandra Pucciarelli
    School of Biosciences and Biotechnology, University of Camerino, Camerino MC, Italy
    PLoS ONE 7:e39694. 2012
    ..the functions of the recently discovered, noncanonical β-like tubulins (BLTs) of the ciliate, Tetrahymena thermophila. Tetrahymena forms 17 distinct microtubular structures whose assembly had been thought to be based on ..
  51. ncbi Development of an Aeromonas hydrophila  infection model using the protozoan Tetrahymena thermophila
    Jing Li
    Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China
    FEMS Microbiol Lett 316:160-8. 2011
    ..have not been well defined, we established an infection model using the free-living, ciliate protozoa Tetrahymena thermophila. The expression of A. hydrophila virulence genes following infection of T...
  52. pmc Comprehensive analysis reveals dynamic and evolutionary plasticity of Rab GTPases and membrane traffic in Tetrahymena thermophila
    Lydia J Bright
    Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, USA
    PLoS Genet 6:e1001155. 2010
    ..Thus, characterizing entire gene families can provide insight into the evolutionary flexibility of fundamental cellular pathways...
  53. ncbi [Expression analysis of 5 hsp70 genes in Tetrahymena thermophila]
    Li Fang Feng
    Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, China
    Dongwuxue Yanjiu 32:267-76. 2011
    Thirteen hsp70 genes with complete conserved domains were identified in Tetrahymena thermophila, and expression of five similar and non-intron hsp70 genes were analyzed...
  54. ncbi Conservation and innovation in Tetrahymena membrane traffic: proteins, lipids, and compartments
    Alejandro D Nusblat
    Cátedra de Biotecnología y Microbiología Industrial, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, C1113AAD Buenos Aires, Argentina
    Methods Cell Biol 109:141-75. 2012
    The past decade has seen a significant expansion in our understanding of membrane traffic in Tetrahymena thermophila, facilitated by the development of new experimental tools and by the availability of the macronuclear genome sequence...
  55. pmc An essential role for the DNA breakage-repair protein Ku80 in programmed DNA rearrangements in Tetrahymena thermophila
    I Ting Lin
    Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 112, Taiwan, Republic of China
    Mol Biol Cell 23:2213-25. 2012
    Programmed DNA rearrangements are important processes present in many organisms. In the ciliated protozoan Tetrahymena thermophila, DNA rearrangements occur during the sexual conjugation process and lead to the deletion of thousands of ..
  56. ncbi Mapping the germ-line and somatic genomes of a ciliated protozoan, Tetrahymena thermophila
    E Orias
    Department of Molecular, Cellular, and Developmental Biology, University of California at Santa Barbara UCSB, Santa Barbara, California 93106, USA
    Genome Res 8:91-9. 1998
    ..b>Tetrahymena thermophila is the only ciliate in which a systematic genetic mapping of DNA polymorphisms has begun...
  57. ncbi A comparison of the polycation receptors of Paramecium tetraurelia and Tetrahymena thermophila
    Eric D Robinette
    Department of Science and Mathematics, Cedarville University, Cedarville, OH 45314, USA
    J Eukaryot Microbiol 55:86-90. 2008
    ..Polycation responses of both organisms are inhibited by neomycin sulfate. While PACAP is the most effective of the three chemorepellents in Tetrahymena, lysozyme is the most effective chemorepellent in Paramecium...
  58. ncbi Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymena
    Kazufumi Mochizuki
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Cell 110:689-99. 2002
    ..We identified a gene, TWI1, in Tetrahymena thermophila that is homologous to piwi and is required for DNA elimination...
  59. ncbi A novel robust heat-inducible promoter for heterologous gene expression in Tetrahymena thermophila
    Ting Yu
    School of Biosciences and Biotechnology, University of Camerino, Camerino, Italy
    Protist 163:284-95. 2012
    ....
  60. ncbi A mutational analysis of conjugation in Tetrahymena thermophila. 2. Phenotypes affecting middle and late development: third prezygotic nuclear division, pronuclear exchange, pronuclear fusion, and postzygotic development
    E S Cole
    Biology Department, St Olaf College, Northfield, Minnesota, 55057, USA
    Dev Biol 189:233-45. 1997
    ..These mutant phenotypes are used to draw inferences regarding developmental dependencies that govern a cell's entry into the postzygotic and exconjugant developmental programs...
  61. pmc Holoenzyme proteins required for the physiological assembly and activity of telomerase
    Keren L Witkin
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3204, USA
    Genes Dev 18:1107-18. 2004
    ..These findings demonstrate that telomerase holoenzyme proteins other than TERT play critical roles in RNP biogenesis and function...
  62. ncbi Profilin functions in cytokinesis, nuclear positioning, and stomatogenesis in Tetrahymena thermophila
    David E Wilkes
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    J Eukaryot Microbiol 50:252-62. 2003
    Expression of the actin-binding protein profilin was disrupted in the ciliate Tetrahymena thermophila by an antisense ribosome method. In cells with the antisense disruption no profilin protein was detected...
  63. pmc A beta-tubulin mutation selectively uncouples nuclear division and cytokinesis in Tetrahymena thermophila
    Joshua J Smith
    Department of Pharmacology, Medical School, University of Minnesota, 6 120 Jackson Hall, 321 Church Street S E, Minneapolis, MN 55455, USA
    Eukaryot Cell 3:1217-26. 2004
    The ciliated protozoan Tetrahymena thermophila contains two distinct nuclei within a single cell-the mitotic micronucleus and the amitotic macronucleus...
  64. pmc Genome-wide identification and evolution of ATP-binding cassette transporters in the ciliate Tetrahymena thermophila: A case of functional divergence in a multigene family
    Jie Xiong
    Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, PR China
    BMC Evol Biol 10:330. 2010
    ..Owing to their medical and toxicological importance, members of the ABC superfamily have been studied in several model organisms and warrant examination in newly sequenced genomes...
  65. ncbi Monovalent cations mediate formation of native tertiary structure of the Tetrahymena thermophila ribozyme
    Keiji Takamoto
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nat Struct Biol 9:928-33. 2002
    ....
  66. pmc Tetrahymena telomerase protein p65 induces conformational changes throughout telomerase RNA (TER) and rescues telomerase reverse transcriptase and TER assembly mutants
    Andrea J Berman
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Mol Cell Biol 30:4965-76. 2010
    ..Second, p65 acts as a molecular buttress within the assembled RNP, mutually stabilizing TER and TERT in catalytically active conformations...
  67. ncbi Dynein light chain family in Tetrahymena thermophila
    David E Wilkes
    Department of Biology, Harvey Mudd College, Claremont, California 91711 5990, USA
    Cell Motil Cytoskeleton 64:82-96. 2007
    ..We have searched the recently sequenced Tetrahymena thermophila macronuclear genome to describe the entire family of dynein light chains expressed in this organism...
  68. ncbi Multiple monovalent ion-dependent pathways for the folding of the L-21 Tetrahymena thermophila ribozyme
    Takeshi Uchida
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Mol Biol 328:463-78. 2003
    ..The Mg(2+)-dependent folding of the L-21 Sca 1 Tetrahymena thermophila ribozyme has been followed using this technique at an elevated concentration of monovalent ion (200 mM ..
  69. ncbi Electron tomography and immuno-labeling of Tetrahymena thermophila basal bodies
    Thomas H Giddings
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Methods Cell Biol 96:117-41. 2010
    ..The ciliate Tetrahymena thermophila has more than 700 basal bodies per cell, making it an excellent choice for the study of the structure, ..
  70. pmc Centromeric histone H3 is essential for vegetative cell division and for DNA elimination during conjugation in Tetrahymena thermophila
    Bowen Cui
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Mol Cell Biol 26:4499-510. 2006
    The Tetrahymena thermophila CNA1 gene encodes the centromeric H3, Cna1p...
  71. pmc Class I histone deacetylase Thd1p affects nuclear integrity in Tetrahymena thermophila
    Emily A Wiley
    W M Keck Joint Science Department, Claremont Colleges, 925 N Mills Ave, Claremont, CA 91711, USA
    Eukaryot Cell 4:981-90. 2005
    ..We previously identified a macronuclear-specific class I HDAC in Tetrahymena thermophila called Thd1p, which is expressed early in the development of the macronucleus when it initially becomes ..
  72. pmc The CNA1 histone of the ciliate Tetrahymena thermophila is essential for chromosome segregation in the germline micronucleus
    Marcella D Cervantes
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Mol Biol Cell 17:485-97. 2006
    ..To study ciliate chromosome segregation, we have identified the centromeric histone gene in the Tetrahymena thermophila genome (CNA1)...
  73. ncbi Cloning and sequencing of four new metallothionein genes from Tetrahymena thermophila and T. pigmentosa: evolutionary relationships in Tetrahymena MT family
    Francesco Boldrin
    Department of Biology, University of Padova, Via U Bassi 58 B, I 35131 Padova, Italy
    Protist 154:431-42. 2003
    The structure of four new MT (metallothionein) genes of Tetrahymena thermophila and T. pigmentosa were characterized...
  74. pmc Phosphorylation of the SQ H2A.X motif is required for proper meiosis and mitosis in Tetrahymena thermophila
    Xiaoyuan Song
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Mol Cell Biol 27:2648-60. 2007
    ..These results demonstrate multiple roles of H2A.X phosphorylation in maintaining genomic integrity in different phases of the Tetrahymena life cycle...
  75. pmc The transition from conjugal development to the first vegetative cell division is dependent on RAD51 expression in the ciliate Tetrahymena thermophila
    T C Marsh
    Department of Pharmacology, Medical School, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Genetics 157:1591-8. 2001
    ..DNA and is involved in both genetic recombination and double-strand break repair in the ciliate Tetrahymena thermophila. We have previously shown that disruption of the Tetrahymena RAD51 somatic macronuclear locus leads to ..
  76. ncbi Confocal fluorescence microscopy for Tetrahymena thermophila
    E S Cole
    Biology Department, St Olaf College, Northfield, Minnesota 55057, USA
    Methods Cell Biol 70:337-59. 2002
  77. pmc Polymorphism and selection at the SerH immobilization antigen locus in natural populations of Tetrahymena thermophila
    Carri A Gerber
    Department of Biological, Geological and Environmental Sciences, Cleveland State University, Cleveland, Ohio 44115, USA
    Genetics 160:1469-79. 2002
    The SerH locus of Tetrahymena thermophila is one of several paralogous loci with genes encoding variants of the major cell surface protein known as the immobilization antigen (i-ag)...
  78. pmc Role of histone deacetylation in developmentally programmed DNA rearrangements in Tetrahymena thermophila
    Sandra Duharcourt
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Eukaryot Cell 1:293-303. 2002
    ..We propose a model in which underacetylated nucleosomes mark germ line-limited sequences for excision...
  79. ncbi Cloning and characterization of the gene encoding the highly expressed ribosomal protein l3 of the ciliated protozoan Tetrahymena thermophila. Evidence for differential codon usage in highly expressed genes
    L K Larsen
    Department of Molecular Biology, Odense University, Campusvej 55, Odense M, DK 5260, Denmark
    Cell Biol Int 23:551-60. 1999
    ..the cDNA and the macronuclear genomic copy of the highly conserved ribosomal protein (r-protein) L3 of Tetrahymena thermophila. The r-protein L3 is encoded by a single copy gene interrupted by one intron...
  80. pmc Identification of an essential proximal sequence element in the promoter of the telomerase RNA gene of Tetrahymena thermophila
    B W Hargrove
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
    Nucleic Acids Res 27:4269-75. 1999
    ..are facilitated by the large number of linear DNA molecules found in ciliated protozoa, such as Tetrahymena thermophila. To examine the expression of telomerase, we investigated the transcription of the RNA polymerase III-..
  81. pmc Stepwise protein-mediated RNA folding directs assembly of telomerase ribonucleoprotein
    Michael D Stone
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 446:458-61. 2007
    ..These results identify the RNA folding pathway during telomerase biogenesis and define the mechanism of action for an essential telomerase holoenzyme protein...
  82. pmc Tetrahymena Genome Database (TGD): a new genomic resource for Tetrahymena thermophila research
    Nicholas A Stover
    Department of Genetics, School of Medicine, Stanford University, Stanford, CA 94305 5120, USA
    Nucleic Acids Res 34:D500-3. 2006
    ..ciliate.org) for researchers studying the model ciliate organism Tetrahymena thermophila. Employing the underlying database structure and programming of the Saccharomyces Genome Database, the ..
  83. pmc The domain architecture of large guanine nucleotide exchange factors for the small GTP-binding protein Arf
    Barbara Mouratou
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    BMC Genomics 6:20. 2005
    ..In contrast, the function of non-catalytic domains and how they regulate/cooperate with the catalytic domain is essentially unknown...
  84. pmc New Tetrahymena basal body protein components identify basal body domain structure
    Chandra L Kilburn
    Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, CO 80309, USA
    J Cell Biol 178:905-12. 2007
    ..body proteome and determine the specific localization of basal body components in the ciliated protozoan Tetrahymena thermophila. Using a biochemical, bioinformatic, and genetic approach, we identify 97 known and candidate basal body ..
  85. ncbi Crystal structure of a group I ribozyme domain: principles of RNA packing
    J H Cate
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Science 273:1678-85. 1996
    ..The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described...
  86. pmc The reverse transcriptase component of the Tetrahymena telomerase ribonucleoprotein complex
    K Collins
    Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, CA 94720 3204, USA
    Proc Natl Acad Sci U S A 95:8485-90. 1998
    ..These experiments suggest a complexity of telomerase structure and function...
  87. pmc Highly divergent mitochondrial ATP synthase complexes in Tetrahymena thermophila
    Praveen Balabaskaran Nina
    Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS Biol 8:e1000418. 2010
    ..nature of ATP synthase in Apicomplexa, genes for these essential components were absent even in Tetrahymena thermophila, a free-living ciliate belonging to a sister clade of Apicomplexa, which demonstrates robust oxidative ..
  88. pmc A class II histone deacetylase acts on newly synthesized histones in Tetrahymena
    Joshua J Smith
    W M Keck Science Center, 925 N Mills Ave, Claremont, CA 91711, USA
    Eukaryot Cell 7:471-82. 2008
    ..In the ciliate Tetrahymena thermophila, transcription-related deacetylation in the macronucleus is physically separated from deposition-related ..
  89. ncbi Chemoattraction to lysophosphatidic acid does not require a change in membrane potential in Tetrahymena thermophila
    Thomas J Lampert
    Department of Biological Sciences, University at Buffalo, Amherst, NY 14260, U S A
    Cell Biol Int 35:519-28. 2011
    ..These combined behavioural and electrophysiological analyses support the novel hypothesis that chemoattraction to some attractants, like LPA, can occur without hyperpolarization and increased swim speed in Tetrahymena...
  90. ncbi Differentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR
    Lifang Feng
    State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China
    Aquat Toxicol 81:99-105. 2007
    ..We constructed the suppression subtractive hybridization library of Tetrahymena thermophila exposed to TBT, and screened out 101 Expressed Sequence Tags whose expressions were significantly up- or ..
  91. pmc The Tetrahymena thermophila phagosome proteome
    Mary Ellen Jacobs
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    Eukaryot Cell 5:1990-2000. 2006
    ..In contrast, unicellular eukaryotes, such as the ciliate Tetrahymena thermophila, employ phagocytosis to ingest and degrade other microorganisms to meet their nutritional needs...
  92. pmc The two SAS-6 homologs in Tetrahymena thermophila have distinct functions in basal body assembly
    Brady P Culver
    Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, CO 80309, USA
    Mol Biol Cell 20:1865-77. 2009
    ..To address this question we used Tetrahymena thermophila, which expresses two SAS-6 homologues, TtSAS6a and TtSAS6b...
  93. ncbi Progeny of germ line knockouts of ASI2, a gene encoding a putative signal transduction receptor in Tetrahymena thermophila, fail to make the transition from sexual reproduction to vegetative growth
    Shuqiang Li
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53201 1881, USA
    Dev Biol 295:633-46. 2006
    ..The germ line ASI2 null phenotype is rescued in a mating of a knockout strain with wild type cells...
  94. ncbi Tubulin polyglutamylase enzymes are members of the TTL domain protein family
    Carsten Janke
    Centre de Recherches de Biochimie Macromoleculaire, CNRS, 34293 Montpellier, France
    Science 308:1758-62. 2005
    ..In the model protist Tetrahymena thermophila, two conserved types of polyglutamylases were characterized that differ in substrate preference and ..
  95. pmc Structure of the Tetrahymena thermophila telomerase RNA helix II template boundary element
    Rebecca J Richards
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 1569, USA
    Nucleic Acids Res 34:816-25. 2006
    ..Here, we report the solution structure of the TBE element (helix II) from Tetrahymena thermophila telomerase RNA...
  96. pmc Induction of gene silencing by hairpin RNA expression in Tetrahymena thermophila reveals a second small RNA pathway
    Rachel A Howard-Till
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Seattle, WA 98109, USA
    Mol Cell Biol 26:8731-42. 2006
    ..causes gene silencing, RNA interference (RNAi) has been linked to programmed DNA deletion in the ciliate Tetrahymena thermophila. Here we have developed an efficient method to inducibly express double-stranded RNA hairpins and ..
  97. pmc Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila
    Chengjie Fu
    Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, PR China
    BMC Genomics 10:208. 2009
    ....
  98. pmc A non-long terminal repeat retrotransposon family is restricted to the germ line micronucleus of the ciliated protozoan Tetrahymena thermophila
    Jeffrey S Fillingham
    Department of Biology, York University, Toronto M3J 1P3, Ontario, Canada
    Eukaryot Cell 3:157-69. 2004
    The ciliated protozoan Tetrahymena thermophila undergoes extensive programmed DNA rearrangements during the development of a somatic macronucleus from the germ line micronucleus in its sexual cycle...
  99. pmc Studies on the minimal lengths required for DNA primers to be extended by the Tetrahymena telomerase: implications for primer positioning by the enzyme
    Nava Baran
    Department of Biology, Technion Israel Institute of Technology, Haifa 32, 000, Israel
    Nucleic Acids Res 30:5570-8. 2002
    ....
  100. ncbi Methylation of histone h3 at lysine 9 targets programmed DNA elimination in tetrahymena
    Sean D Taverna
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908, USA
    Cell 110:701-11. 2002
    ..These results extend the range of H3(Lys9)Me involvement in chromatin activities outside transcriptional regulation and also strengthen the link between heterochromatin formation and programmed DNA elimination...
  101. ncbi Cytotoxicity of metals common in mining effluent to rainbow trout cell lines and to the ciliated protozoan, Tetrahymena thermophila
    Vivian R Dayeh
    Department of Biology, University of Waterloo, Waterloo, ON, Canada N2L 3G1
    Toxicol In Vitro 19:399-410. 2005
    ..samples for toxicity, two cell lines from rainbow trout, RTgill-W1 and RTL-W1, and the ciliate, Tetrahymena thermophila, were compared for their sensitivity to the toxicity of five metals that are commonly found in mining ..

Research Grants54

  1. Structure and dynamics of the Tetrahymena telomerase ribonucleoprotein
    Michael D Stone; Fiscal Year: 2013
    ..analysis of telomerase we will study the telomerase complex from the well-established model organism Tetrahymena thermophila, using a multifaceted experimental strategy that combines single molecule biophysical techniques paired ..
  2. Structural Studies of Telomerase Holoenzyme
    EDWARD JOHN MIRACCO; Fiscal Year: 2013
    ..The goal of this fellowship will be to acquire a cryoelectron microscopy structure of the Tetrahymena thermophila telomerase holoenzyme complex, assign each component, deduce function from structures obtained at ..
  3. High-throughput Gene Knockout Production in Tetrahymena thermophila
    ROBERT STEPHEN COYNE; Fiscal Year: 2012
    ..coli clones containing gene replacement mutations in approximately 1,300 protein-coding genes of Tetrahymena thermophila and to transform these clones into the Tetrahymena germline genome to generate knockout strains...
  4. The Developmental Genetics of Tetrahymena thermophila.
    Eric S Cole; Fiscal Year: 2010
    ..and molecular mechanisms associated with two dynamic organelles within a unicellular model organism: Tetrahymena thermophila. The nuclear exchange junction represents a molecular theater for studying membrane trafficking and ..
  5. REPLICATION AND AMPLIFICATION OF TETRAHYMENA RDNA
    Geoffrey M Kapler; Fiscal Year: 2010
    ..Our model organism, Tetrahymena thermophila, harbors a 21 kb ribosomal DNA (rDNA) minichromosome that is amplified 9,000-fold during development, but ..
  6. Physical and functional probing of DEAD-box proteins as general RNA chaperones
    Rick Russell; Fiscal Year: 2013
    ..In years 1-5 of funding (2004-2009), we used a well-studied group I intron from Tetrahymena thermophila to show that CYT-19 functions as a true chaperone, facilitating refolding of a long-lived misfolded ..
  7. The structure of telomerase RNA during catalysis
    DAUD COLE; Fiscal Year: 2012
    ..be elucidated by a combination of site-specific mutants and high resolution biochemical techniques using Tetrahymena thermophila telomerase RNA (tTR) as a model...
  8. Novel methods for high-level expression of aglycosylated or fully glycosylated mo
    Paul Colussi; Fiscal Year: 2010
    ..expression platform for the production of monoclonal antibodies in the common pond water ciliate, Tetrahymena thermophila. Successful development of this platform will allow Tetragenetics Inc to make this alternative ..
  9. Tetrahymena Basal Body Duplication
    Mark Winey; Fiscal Year: 2013
    ..steps of basal body assembly by utilizing the cytology and molecular genetic advantages of the ciliate, Tetrahymena thermophila. Basal bodies have the defining structural characteristic of a conserved radial array of nine microtubule ..
  10. The role of Centrins 1 and 2 at Tetrahymena thermophila basal bodies.
    Tyson Vonderfecht; Fiscal Year: 2012
    ..The ciliate protist Tetrahymena thermophila is being used as a system to study centrin function at basal bodies...
  11. ASSET: Advancing Secondary Science Education with Tetrahymena.
    Theodore G Clark; Fiscal Year: 2013
    ..The laboratory units will feature Tetrahymena thermophila, a safe, easily grown single-cell organism that is ideal for demonstrating many of the basic principles ..
  12. A Resource Center for Tetrahymena Thermophila
    Theodore G Clark; Fiscal Year: 2013
    ..A key model for eukaryotic cell and molecular biology, Tetrahymena thermophila has been instrumental to our understanding of a wide range of biological phenomena with direct relevance ..
  13. ncRNA targeted excision of HIV-1 from human cells
    Kevin V Morris; Fiscal Year: 2011
    ..In both human cells and the ciliated protozoa Tetrahymena thermophila, shRNAs are used to guide histone 3 Lysine 9 di-methylation (H3K9me2) to homology containing target loci ..
  14. STRUCTURAL AND FUNCTIONAL ANALYSIS OF TELOMERASE RNAS
    Daniel Romero; Fiscal Year: 1999
    ..RNA (ThR) in the enzymatic activity of telomerase beyond that of a templating function, using the cilate Tetrahymena thermophila as the experimental system. The research plan is logically divided into two objectives...
  15. Analysis of Group I and II Introns in Mammalian Cells
    Bruce Sullenger; Fiscal Year: 2005
    ..In the previous funding cycle of this grant application, we demonstrated that the Tetrahymena thermophila group I intron can perform self- and trans-splicing reactions to alter the sequences of transcripts in ..
  16. CONTROL OF CILIA BIOGENESIS IN TETRAHYMENA THERMOPHILA
    David Pennock; Fiscal Year: 1990
    ..Towards that end two Tetrahymena thermophila mutants with temperature-sensitive defects in both cilia biogenesis and some aspect of the cell cycle ..
  17. THREE SPLICE-SITE OF A SELF-SPLICING GROUP I INTRON
    Richard Waring; Fiscal Year: 1993
    The ribosomal RNA of Tetrahymena thermophila is produced as a precursor containing an intervening sequence (intron) which is able to catalyze its own excision and to join its flanking sequences (exons)...
  18. Mutation accumulation in the ciliate Tetrahymena thermophila
    REBECCA ZUFALL; Fiscal Year: 2013
    ..Here we introduce a new experimental system for elucidating these mutational parameters: the ciliate Tetrahymena thermophila. T...
  19. MOLECULAR GENETICS OF A CELL-CELL RECOGNITION SYSTEM
    Eduardo Orias; Fiscal Year: 1990
    ..is to isolate and characterize the locus of mating type determination in the unicellular eukaryote Tetrahymena thermophila. We propose to walk to the mat locus, starting from the rDNA gene located 30 centimorgans away on ..
  20. ANALYSES OF TETRAHYMENA MUTANTS LACKING OUTER DYNEIN
    David Pennock; Fiscal Year: 1993
    ..into ciliary axonemes and to understand the role(s) of outer arm dyneins in ciliary motility by using a Tetrahymena thermophila mutant that is nonmotile and that lacks outer arm dyneins as a model system...
  21. REGULATION OF RDNA REPLICATION IN TETRAHYMENA
    DRENA LARSON; Fiscal Year: 1993
    ..by focusing on the replication of the amplified rRNA genes (rDNA) in the single-celled protozoan, Tetrahymena thermophila. The proposed experiments investigate the contrdl of rDNA replication in two ways...
  22. MECHANISMS OF FUNCTIONAL SPECIALIZATION OF MICROTUBULES
    Jacek Gaertig; Fiscal Year: 2000
    ..proposal is to study the significance of post- translational modifications of tubulin in the ciliate, Tetrahymena thermophila. The project is based upon a recent achievement by the principal investigator, while he was a ..
  23. ANALYSIS OF SPATIAL PATTERNING IN A UNICELLULAR ORGANISM
    Joseph Frankel; Fiscal Year: 1993
    ..The specific aim is to analyze the arrangement of structures in the cell surface region of a ciliate, Tetrahymena thermophila. Previous studies of hereditary reversals of asymmetry in the arrangement of cell-surface structures led ..
  24. Tetrahymena Genome Sequencing Project
    Jonathan Eisen; Fiscal Year: 2005
    b>Tetrahymena thermophila is an excellent microbial organism for fundamental eukaryotic experimental cell and molecular biology and for comparative and functional genomics...
  25. Function and Regulation of Inner Arm Dyneins
    David Pennock; Fiscal Year: 2005
    ..into six genes encoding the 1-headed inner arm dynein heavy chains in the unicellular protozoan, Tetrahymena thermophila. The specific aims of this project are: I...
  26. STRUCTURAL BIOLOGY OF RNA FOLDING
    MARK CHANCE; Fiscal Year: 2003
    ..The proposed equilibrium and time-resolved folding studies of the 385 nucleotide L-21 Sca I Tetrahymena thermophila ribozyme will yield a thermodynamic and kinetic picture of the formation of the individual tertiary ..
  27. RNAi in DNA Sequence Elimination in Tetrahymena
    Martin A Gorovsky; Fiscal Year: 2010
    ..These studies should shed light on other RNAi- like processes that are important for normal development and maintenance of genome stability in multicellular organisms. ..
  28. Repeated Genes: Organization, Evolution, and Function
    MARTIN GOROVSKY; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  29. CONTROL OF GENE ACTIVITY AND ORGANELLE BIOGENESIS
    MARTIN GOROVSKY; Fiscal Year: 2006
    ..The lower eukaryote, Tetrahymena thermophila, is used as a model...
  30. MECHANISMS OF REGULATED EXOCYTOSIS IN TETRAHYMENA
    AARON TURKEWITZ; Fiscal Year: 2001
    ..of proteins involved in the biosynthesis and exocytosis of dense-core granules in the ciliated protist, Tetrahymena thermophila. Granule assembly involves the ordered condensation of a set of granule proteins beginning in the trans-..
  31. Forward Genetics in Tetrahymena: Cloning by Complementation
    ROBERT COYNE; Fiscal Year: 2008
    unreadable] DESCRIPTION (provided by applicant): The ciliated protozoan Tetrahymena thermophila is a model organism for cellular and molecular biology that has contributed too many discoveries of fundamental importance in basic and ..
  32. Biogenesis of Dense Core Secretory Granules in Tetrahymena
    AARON TURKEWITZ; Fiscal Year: 2009
    ..We will do this using the ciliate Tetrahymena thermophila, a genetically-manipulable single-celled system...
  33. Identification of Tetrahymena DNA Rearrangement Genes
    DOUGLAS LEE CHALKER; Fiscal Year: 2010
    The ciliate Tetrahymena thermophila excises approximately 6000 specific DNA segments from its somatic nucleus during development...
  34. FUNCTIONAL ANALYSIS OF CALCIUM STORES IN TETRAHYMENA
    AARON TURKEWITZ; Fiscal Year: 2003
    ..The ciliate Tetrahymena thermophila offers a host of experimental advantages for studying such mechanisms...
  35. FOOTPRINTING WITH IRON (II)-GENERATED HYDROXYL RADICAL
    THOMAS TULLIUS; Fiscal Year: 2003
    ..abstract_text> ..
  36. IDENTITY AND FUNCTION OF VERTEBRATE TELOMERE PROTEINS
    Carolyn Price; Fiscal Year: 2002
    ..We will use a retroviral cDNA expression library to screen for genes that act as high copy suppressors of defects in telomere function. ..
  37. USING THE CHEMISTRY OF IRON(II) TO STUDY DNA STRUCTURE
    THOMAS TULLIUS; Fiscal Year: 2002
    ..The high resolution and structural sensitivity of the hydroxyl radical will be used to determine structural details of these DNA molecules in solution. ..
  38. PURCHASE OF 600MHZ NMR SPECTROMETER
    Juli Feigon; Fiscal Year: 2001
    ..abstract_text> ..
  39. SPINDLE POLES AND NUCLEAR PORES--YEAST NDC1 AND MPS2
    Mark Winey; Fiscal Year: 2003
    ..The proposed project is aimed at understanding the function of a set of unusual membrane proteins at the SPB and the NPC, and at identifying other proteins with which the interact at these organelles. ..
  40. CONFORMATIONAL VARIABILITY OF RNA
    JULI F FEIGON; Fiscal Year: 2010
    ..Our structural studies will provide molecular insight into how non-coding RNAs are regulated for cell function. ..
  41. Frontiers in NMR: A Celebration of the UCLA MRR
    Juli Feigon; Fiscal Year: 2003
    ..The Seaborg Event attracts a wide audience from southern California, as well as many alumni from out-of-state, and will thus showcase the new facility to an audience that extends beyond the magnetic resonance scientific community. ..
  42. Chromosome breakage and DNA detection in Tetrahymena
    Meng Chao Yao; Fiscal Year: 2006
    ..This proposal will examine this type of DNA rearrangements in the model eukaryote Tetrahymena thermophila. Past studies of this project have shown that the somatic genome of this organism is dramatically re-..
  43. Conference Proposal to support FASEB Conference on Ciliate Molecular Biology
    Carolyn Price; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  44. Automated Macromolecular Crystallization System
    JEFFREY KIEFT; Fiscal Year: 2007
    ..Cost savings. Cost of crystallization trials will decrease due to the use of smaller amounts of reagents, including solutions and protein/RNA samples. [unreadable] [unreadable] [unreadable]..
  45. Development of tools for structural genomics of viral RNA
    JEFFREY KIEFT; Fiscal Year: 2008
    ..By solving the structures of these RNAs, we will inform and enable new strategies for developing therapeutics aimed at curing viral disease. [unreadable] [unreadable] [unreadable]..
  46. PHYSICAL CHEMISTRY OF NUCLEIC ACIDS
    Carlos Bustamante; Fiscal Year: 2008
    ..Finally, in the experiments described here, mechanical force will be used as a new controllable variable to characterize the process of mechanochemical transduction in this motor enzyme. ..
  47. TETRAHYMENA GENOME PROJECT
    Eduardo Orias; Fiscal Year: 2008
    unreadable] DESCRIPTION (provided by applicant): Tetrahymena thermophila is a very useful genetic, unicellular, animal model for fundamental eukaryotic experimental cell and molecular biology and for comparative genomics...
  48. Function & Regulation of two Novel PARPs, tankyrase 1&2
    Carolyn Price; Fiscal Year: 2008
    ..Aim 4 will investigate the in vivo regulation of tankyrase PARP activity. We will examine the in vivo levels of tankyrase poly(ADP-ribosyl)ation and whether this is regulated by phosphorylation. ..
  49. A Unified Reporter Gene for Multi-Modality Imaging
    Jianghong Rao; Fiscal Year: 2005
    ..abstract_text> ..
  50. Developmental regulation of DNA replication during programmed gene ampplification
    Geoffrey Kapler; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  51. Tetrahymena Basal Body Duplication
    Mark Winey; Fiscal Year: 2004
    ....
  52. DNA MODIFICATION/RESTRICTION IN PRO-AND EUKARYOTES
    Stanley Hattman; Fiscal Year: 2001
    ..We also propose to initiate new research on the [N6-amino] DNA-MTase in the eukaryotic protozoan. Tetrahymena thermophila, to investigate if it has an essential biological function; e.g...
  53. RATIONAL DESIGN TOOLS FOR ANTISENSE NUCLEIC ACIDS
    Ye Ding; Fiscal Year: 2007
    ..In the post-genomic era, the availability of the software and the Web server will substantially facilitate applications of antisense nucleic acids in high throughput functional genomics. ..
  54. Structure of genomic DNA at single-nucleotide resolution
    Thomas D Tullius; Fiscal Year: 2010
    ..Experimental data production and informatics pipelines, data verification and validation protocols, and plans for data deposition are detailed in the application. ..