caenorhabditis elegans

Summary

Summary: A species of nematode that is widely used in biological, biochemical, and genetic studies.

Top Publications

  1. ncbi Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
    A Fire
    Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Nature 391:806-11. 1998
  2. ncbi The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
    B J Reinhart
    Department of Molecular Biology, Massachusetts General Hospital, and Harvard Medical School, Boston 02114, USA
    Nature 403:901-6. 2000
  3. ncbi Collective dynamics of 'small-world' networks
    D J Watts
    Department of Theoretical and Applied Mechanics, Cornell University, Ithaca, New York 14853, USA
    Nature 393:440-2. 1998
  4. pmc Integrative analysis of the Caenorhabditis elegans genome by the modENCODE project
    Mark B Gerstein
    Program in Computational Biology and Bioinformatics, Yale University, Bass 432, 266 Whitney Avenue, New Haven, CT 06520, USA
    Science 330:1775-87. 2010
  5. ncbi The evolutionary fate and consequences of duplicate genes
    M Lynch
    Department of Biology, University of Oregon, Eugene, OR 97403, USA
    Science 290:1151-5. 2000
  6. ncbi Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA
    A E Pasquinelli
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Nature 408:86-9. 2000
  7. ncbi RAS is regulated by the let-7 microRNA family
    Steven M Johnson
    Department of Molecular, Cellular and Developmental Biology, Yale University, P O Box 208103, New Haven, CT 06520, USA
    Cell 120:635-47. 2005
  8. pmc Single-copy insertion of transgenes in Caenorhabditis elegans
    Christian Frøkjaer-Jensen
    Department of Biology and Howard Hughes Medical Institute, University of Utah, 257 South 1400 East, Salt Lake City, Utah 84112, USA
    Nat Genet 40:1375-83. 2008
  9. pmc WormBase 2012: more genomes, more data, new website
    Karen Yook
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Nucleic Acids Res 40:D735-41. 2012
  10. ncbi Network motifs: simple building blocks of complex networks
    R Milo
    Departments of Physics of Complex Systems and Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel 76100
    Science 298:824-7. 2002

Detail Information

Publications290 found, 100 shown here

  1. ncbi Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
    A Fire
    Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Nature 391:806-11. 1998
    ..RNA interference has been used in the nematode Caenorhabditis elegans to manipulate gene expression...
  2. ncbi The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
    B J Reinhart
    Department of Molecular Biology, Massachusetts General Hospital, and Harvard Medical School, Boston 02114, USA
    Nature 403:901-6. 2000
    ..We propose that the sequential stage-specific expression of the lin-4 and let-7 regulatory RNAs triggers transitions in the complement of heterochronic regulatory proteins to coordinate developmental timing...
  3. ncbi Collective dynamics of 'small-world' networks
    D J Watts
    Department of Theoretical and Applied Mechanics, Cornell University, Ithaca, New York 14853, USA
    Nature 393:440-2. 1998
    ..The neural network of the worm Caenorhabditis elegans, the power grid of the western United States, and the collaboration graph of film actors are shown to be ..
  4. pmc Integrative analysis of the Caenorhabditis elegans genome by the modENCODE project
    Mark B Gerstein
    Program in Computational Biology and Bioinformatics, Yale University, Bass 432, 266 Whitney Avenue, New Haven, CT 06520, USA
    Science 330:1775-87. 2010
    We systematically generated large-scale data sets to improve genome annotation for the nematode Caenorhabditis elegans, a key model organism...
  5. ncbi The evolutionary fate and consequences of duplicate genes
    M Lynch
    Department of Biology, University of Oregon, Eugene, OR 97403, USA
    Science 290:1151-5. 2000
    ..Although duplicate genes may only rarely evolve new functions, the stochastic silencing of such genes may play a significant role in the passive origin of new species...
  6. ncbi Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA
    A E Pasquinelli
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Nature 408:86-9. 2000
    Two small RNAs regulate the timing of Caenorhabditis elegans development...
  7. ncbi RAS is regulated by the let-7 microRNA family
    Steven M Johnson
    Department of Molecular, Cellular and Developmental Biology, Yale University, P O Box 208103, New Haven, CT 06520, USA
    Cell 120:635-47. 2005
    ..let-7 expression is lower in lung tumors than in normal lung tissue, while RAS protein is significantly higher in lung tumors, providing a possible mechanism for let-7 in cancer...
  8. pmc Single-copy insertion of transgenes in Caenorhabditis elegans
    Christian Frøkjaer-Jensen
    Department of Biology and Howard Hughes Medical Institute, University of Utah, 257 South 1400 East, Salt Lake City, Utah 84112, USA
    Nat Genet 40:1375-83. 2008
    At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA...
  9. pmc WormBase 2012: more genomes, more data, new website
    Karen Yook
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Nucleic Acids Res 40:D735-41. 2012
    ..We report on the growth of our database and on our work in keeping pace with the growing demand for data, efforts to anticipate the requirements of users and new collaborations with the larger science community...
  10. ncbi Network motifs: simple building blocks of complex networks
    R Milo
    Departments of Physics of Complex Systems and Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel 76100
    Science 298:824-7. 2002
    ..describe elements as different as biomolecules within a cell and synaptic connections between neurons in Caenorhabditis elegans. Motifs may thus define universal classes of networks...
  11. ncbi Processing of primary microRNAs by the Microprocessor complex
    Ahmet M Denli
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 432:231-5. 2004
    ..Suppression of Pasha expression in Drosophila cells or Caenorhabditis elegans interferes with pri-miRNA processing, leading to an accumulation of pri-miRNAs and a reduction in mature ..
  12. ncbi IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA
    Marcella Calfon
    Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Nature 415:92-6. 2002
    ..that mutations in either ire-1 or the transcription-factor-encoding xbp-1 gene abolished the UPR in Caenorhabditis elegans. Mammalian XBP-1 is essential for immunoglobulin secretion and development of plasma cells, and high ..
  13. ncbi Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
    Ravi S Kamath
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nature 421:231-7. 2003
    ..Our resulting data set and reusable RNAi library of 16,757 bacterial clones will facilitate systematic analyses of the connections among gene sequence, chromosomal location and gene function in C. elegans...
  14. ncbi C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation
    A C Epstein
    The Henry Wellcome Building of Genomic Medicine, Roosevelt Drive, Oxford OX3 7BN, United Kingdom
    Cell 107:43-54. 2001
    ....
  15. ncbi Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans
    Coleen T Murphy
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143 2200, USA
    Nature 424:277-83. 2003
    ..DAF-16, a FOXO-family transcription factor, influences the rate of ageing of Caenorhabditis elegans in response to insulin/insulin-like growth factor 1 (IGF-I) signalling...
  16. ncbi daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans
    K D Kimura
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Science 277:942-6. 1997
    ..Life-span regulation by insulin-like metabolic control is analogous to mammalian longevity enhancement induced by caloric restriction, suggesting a general link between metabolism, diapause, and longevity...
  17. pmc Evidence for compensatory upregulation of expressed X-linked genes in mammals, Caenorhabditis elegans and Drosophila melanogaster
    Xinxian Deng
    Department of Pathology, University of Washington School of Medicine, Seattle, USA
    Nat Genet 43:1179-85. 2011
    ..elegans and Drosophila. Our analyses, which take into account the skewed gene content of the X chromosome, support the hypothesis of upregulation of expressed X-linked genes to balance expression of the genome...
  18. pmc Imaging individual mRNA molecules using multiple singly labeled probes
    Arjun Raj
    Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Methods 5:877-9. 2008
    ..We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons...
  19. pmc Population dynamics and habitat sharing of natural populations of Caenorhabditis elegans and C. briggsae
    Marie Anne Felix
    Institut Jacques Monod, CNRS University of Paris VII, France
    BMC Biol 10:59. 2012
    The nematode Caenorhabditis elegans is a major model organism in laboratory biology. Very little is known, however, about its ecology, including where it proliferates. In the past, C...
  20. pmc Crystal structure of the multidrug transporter P-glycoprotein from Caenorhabditis elegans
    Mi Sun Jin
    Department of Biological Sciences, Purdue University, Indiana 47907, USA
    Nature 490:566-9. 2012
    ..Here we present biochemical characterization of P-gp from Caenorhabditis elegans and its crystal structure at a resolution of 3.4 ångströms...
  21. pmc COSA-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers
    Rayka Yokoo
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 149:75-87. 2012
    ..COSA-1 foci serve as a unique live cell readout for investigating CO formation and CO interference...
  22. ncbi An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans
    N C Lau
    Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 294:858-62. 2001
    Two small temporal RNAs (stRNAs), lin-4 and let-7, control developmental timing in Caenorhabditis elegans. We find that these two regulatory RNAs are members of a large class of 21- to 24-nucleotide noncoding RNAs, called microRNAs (..
  23. pmc The cell-non-autonomous nature of electron transport chain-mediated longevity
    Jenni Durieux
    The Glenn Center for Aging Research, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cell 144:79-91. 2011
    ..These results suggest that mitochondria may establish and perpetuate the rate of aging for the whole organism independent of cell-autonomous functions...
  24. pmc Differential chromatin marking of introns and expressed exons by H3K36me3
    Paulina Kolasinska-Zwierz
    The Gurdon Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Genet 41:376-81. 2009
    ..To provide a framework for the analysis of chromatin function in Caenorhabditis elegans, we generated a genome-wide map of histone H3 tail methylations. We find that C...
  25. pmc A genome-scale resource for in vivo tag-based protein function exploration in C. elegans
    Mihail Sarov
    TransgeneOmics Unit, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany
    Cell 150:855-66. 2012
    ..a genome-scale transgenic platform for in vivo expression of fluorescent- and affinity-tagged proteins in Caenorhabditis elegans under endogenous cis regulatory control...
  26. ncbi A molecular evolutionary framework for the phylum Nematoda
    M L Blaxter
    Institute of Cell, Animal and Population Biology, University of Edinburgh, UK
    Nature 392:71-5. 1998
    ..a global scale; interstitial nematodes pervade sediment and soil ecosystems in overwhelming numbers; and Caenorhabditis elegans is a favourite experimental model system...
  27. pmc BioGRID: a general repository for interaction datasets
    Chris Stark
    Ontario Cancer Institute, Princess Margaret Hospital, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9
    Nucleic Acids Res 34:D535-9. 2006
    ..thebiogrid.org. BioGRID release version 2.0 includes >116 000 interactions from Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster and Homo sapiens...
  28. ncbi The let-7 family of microRNAs
    Sarah Roush
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Trends Cell Biol 18:505-16. 2008
    The first two known microRNAs (miRNAs), lin-4 and let-7, were originally discovered in the nematode Caenorhabditis elegans and control the timing of stem-cell division and differentiation...
  29. ncbi Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing
    A Grishok
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 106:23-34. 2001
    ..In Caenorhabditis elegans, lin-4 and let-7 encode small temporal RNAs (stRNAs) of 22 nt that regulate stage-specific development...
  30. pmc Formation, regulation and evolution of Caenorhabditis elegans 3'UTRs
    Calvin H Jan
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Nature 469:97-101. 2011
    ..Although crucial roles for 3'UTR-mediated gene regulation have been found in Caenorhabditis elegans, most C. elegans genes have lacked annotated 3'UTRs...
  31. ncbi Multimodal fast optical interrogation of neural circuitry
    Feng Zhang
    Department of Bioengineering, Stanford University, Stanford, California 94305, USA
    Nature 446:633-9. 2007
    ..Likewise, NpHR and ChR2 can be targeted together to Caenorhabditis elegans muscle and cholinergic motor neurons to control locomotion bidirectionally...
  32. ncbi Alternative Perspectives on Aging in Caenorhabditis elegans: Reactive Oxygen Species or Hyperfunction?
    David Gems
    Institute of Healthy Ageing and Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom
    Antioxid Redox Signal 19:321-9. 2013
    ..This theory also predicts that processes that protect against oxidative damage (involving detoxification, repair, and turnover) protect against aging and increase lifespan...
  33. pmc Chromosome-scale selective sweeps shape Caenorhabditis elegans genomic diversity
    Erik C Andersen
    Lewis Sigler Institute for Integrative Genomics, Princeton University, New Jersey, USA
    Nat Genet 44:285-90. 2012
    The nematode Caenorhabditis elegans is central to research in molecular, cell and developmental biology, but nearly all of this research has been conducted on a single strain of C. elegans...
  34. pmc Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans
    R S Kamath
    Wellcome CRC Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK
    Genome Biol 2:RESEARCH0002. 2001
    In Caenorhabditis elegans, injection of double-stranded RNA (dsRNA) results in the specific inactivation of genes containing homologous sequences, a technique termed RNA-mediated interference (RNAi)...
  35. pmc Transgenerational epigenetic inheritance of longevity in Caenorhabditis elegans
    Eric L Greer
    Department of Genetics, Stanford University, 300 Pasteur Drive, Stanford, California 94305, USA
    Nature 479:365-71. 2011
    ..H3K4me3) complex, composed of ASH-2, WDR-5 and the histone methyltransferase SET-2, regulates Caenorhabditis elegans lifespan...
  36. ncbi Green fluorescent protein as a marker for gene expression
    M Chalfie
    Department of Biological Sciences, Columbia University, New York, NY 10027
    Science 263:802-5. 1994
    ..GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells...
  37. ncbi Opposing activities protect against age-onset proteotoxicity
    Ehud Cohen
    Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 313:1604-10. 2006
    ..Aggregation-mediated Abeta(1-42) toxicity was reduced in Caenorhabditis elegans when aging was slowed by decreased insulin/insulin growth factor-1-like signaling (IIS)...
  38. ncbi An extensive class of small RNAs in Caenorhabditis elegans
    R C Lee
    Dartmouth Medical School, Department of Genetics, Hanover, NH 03755, USA
    Science 294:862-4. 2001
    ..lin-4 and let-7 antisense RNAs are temporal regulators that control the timing of developmental events in Caenorhabditis elegans by inhibiting translation of target mRNAs...
  39. ncbi Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans
    H A Tissenbaum
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Nature 410:227-30. 2001
    In Caenorhabditis elegans, mutations that reduce the activity of an insulin-like receptor (daf-2) or a phosphatidylinositol-3-OH kinase (age-1) favour entry into the dauer state during larval development and extend lifespan in adults...
  40. ncbi Regulation of aging and age-related disease by DAF-16 and heat-shock factor
    Ao Lin Hsu
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143 2200, USA
    Science 300:1142-5. 2003
    The Caenorhabditis elegans transcription factor HSF-1, which regulates the heat-shock response, also influences aging...
  41. ncbi Lifespan extension by conditions that inhibit translation in Caenorhabditis elegans
    Malene Hansen
    Department of Biochemistry and Biophysics, University of California, 600 16th Street, San Francisco, CA 94158, USA
    Aging Cell 6:95-110. 2007
    ..proteins, ribosomal-protein S6 kinase or translation-initiation factors increases the lifespan of Caenorhabditis elegans. These perturbations, as well as inhibition of the nutrient sensor target of rapamycin (TOR), which is ..
  42. ncbi Glucose restriction extends Caenorhabditis elegans life span by inducing mitochondrial respiration and increasing oxidative stress
    Tim J Schulz
    Department of Human Nutrition, Institute of Nutrition, University of Jena, D 07743 Jena, Germany
    Cell Metab 6:280-93. 2007
    ..We show here that increased glucose availability decreases Caenorhabditis elegans life span, while impaired glucose metabolism extends life expectancy by inducing mitochondrial respiration...
  43. pmc Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like signaling in C. elegans
    Jennifer M A Tullet
    Section on Developmental and Stem Cell Biology, Joslin Diabetes Center Department of Pathology, Harvard Medical School Harvard Stem Cell Institute, 1 Joslin Place, Boston, MA 02215, USA
    Cell 132:1025-38. 2008
    ..Furthermore, SKN-1 that is constitutively active increases life span independently of DAF-16. Our findings indicate that the transcription network regulated by SKN-1 promotes longevity and is an important direct target of IIS...
  44. pmc TOR signaling and rapamycin influence longevity by regulating SKN-1/Nrf and DAF-16/FoxO
    Stacey Robida-Stubbs
    Joslin Diabetes Center, Harvard Stem Cell Institute, and Department of Genetics, Harvard Medical School, Boston, MA 02215, USA
    Cell Metab 15:713-24. 2012
    ..We conclude that modulation of SKN-1/Nrf and DAF-16/FoxO may be generally important in the effects of TOR signaling in vivo and that these transcription factors mediate an opposing relationship between growth signals and longevity...
  45. pmc OrthoList: a compendium of C. elegans genes with human orthologs
    Daniel D Shaye
    Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, New York, United States of America
    PLoS ONE 6:e20085. 2011
    ..We sought to assess the orthology between C. elegans and human genes to understand better the relationship between their genomes and to generate a compelling list of candidates to streamline RNAi-based screens in this model...
  46. ncbi Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses
    Georg Nagel
    Max Planck Institute for Biophysics, Max von Laue Str 3, D 60438 Frankfurt, Germany
    Curr Biol 15:2279-84. 2005
    ..cation channel from the green alga Chlamydomonas reinhardtii, in excitable cells of the nematode Caenorhabditis elegans, to trigger specific behaviors, simply by illumination...
  47. ncbi Induction of autophagy by spermidine promotes longevity
    Tobias Eisenberg
    Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    Nat Cell Biol 11:1305-14. 2009
    ..Finally, we found that enhanced autophagy is crucial for polyamine-induced suppression of necrosis and enhanced longevity...
  48. pmc WormBase: a comprehensive resource for nematode research
    Todd W Harris
    Ontario Institute for Cancer Research, Toronto, ON, Canada
    Nucleic Acids Res 38:D463-7. 2010
    ..wormbase.org) is a central data repository for nematode biology. Initially created as a service to the Caenorhabditis elegans research field, WormBase has evolved into a powerful research tool in its own right...
  49. ncbi Functional siRNAs and miRNAs exhibit strand bias
    Anastasia Khvorova
    Amgen, Inc, One Amgen Center Drive, Thousand Oaks, CA 91320, USA
    Cell 115:209-16. 2003
    ..Together, these results suggest that the thermodynamic properties of siRNA play a critical role in determining the molecule's function and longevity, possibly biasing the steps involved in duplex unwinding and strand retention by RISC...
  50. pmc Repertoire and evolution of miRNA genes in four divergent nematode species
    Elzo de Wit
    Hubrecht Institute KNAW and University Medical Center Utrecht, Cancer Genomics Center, Utrecht 3584 CT, The Netherlands
    Genome Res 19:2064-74. 2009
    ..We have performed small RNA sequencing in the nematodes Caenorhabditis elegans, C. briggsae, C...
  51. ncbi A conserved MST-FOXO signaling pathway mediates oxidative-stress responses and extends life span
    Maria K Lehtinen
    Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 125:987-1001. 2006
    ....
  52. pmc High-throughput in vivo analysis of gene expression in Caenorhabditis elegans
    Rebecca Hunt-Newbury
    Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS Biol 5:e237. 2007
    ..and generated spatial and temporal tissue expression profiles for 1,886 specific genes in the nematode Caenorhabditis elegans. This effort encompasses about 10% of all genes identified in this organism...
  53. pmc The landscape of C. elegans 3'UTRs
    Marco Mangone
    Center for Genomics and Systems Biology, Department of Biology, New York University, 1009 Silver Center, New York, NY 10003, USA
    Science 329:432-5. 2010
    ..DNA (cDNA) ends, full-length cDNAs, and RNA-seq, we defined approximately 26,000 distinct 3'UTRs in Caenorhabditis elegans for approximately 85% of the 18,328 experimentally supported protein-coding genes and revised ..
  54. pmc Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination
    Daniel J Dickinson
    1 Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA 2 Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Nat Methods 10:1028-34. 2013
    Study of the nematode Caenorhabditis elegans has provided important insights in a wide range of fields in biology. The ability to precisely modify genomes is critical to fully realize the utility of model organisms...
  55. ncbi Cell polarity in eggs and epithelia: parallels and diversity
    Daniel St Johnston
    The Gurdon Institute and the Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 141:757-74. 2010
    ..We also highlight the prospects for future studies on how cortical polarity interfaces with other cellular processes, such as morphogenesis, exocytosis, and lipid signaling, and how defects in polarity contribute to tumor formation...
  56. ncbi Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
    L Timmons
    Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
    Gene 263:103-12. 2001
    ..mediated by double-stranded RNA (RNAi) has been a valuable tool in the analysis of gene function in Caenorhabditis elegans. Here we report an efficient induction of RNAi using bacteria to deliver double-stranded RNA...
  57. ncbi The endocrine regulation of aging by insulin-like signals
    Marc Tatar
    Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, USA
    Science 299:1346-51. 2003
    ....
  58. pmc SKN-1 links C. elegans mesendodermal specification to a conserved oxidative stress response
    Jae Hyung An
    Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 17:1882-93. 2003
    During the earliest stages of Caenorhabditis elegans embryogenesis, the transcription factor SKN-1 initiates development of the digestive system and other mesendodermal tissues. Postembryonic SKN-1 functions have not been elucidated...
  59. ncbi C. elegans locomotory rate is modulated by the environment through a dopaminergic pathway and by experience through a serotonergic pathway
    E R Sawin
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Neuron 26:619-31. 2000
    b>Caenorhabditis elegans modulates its locomotory rate in response to its food, bacteria, in two ways. First, well-fed wild-type animals move more slowly in the presence of bacteria than in the absence of bacteria...
  60. ncbi Tissue-specific activities of C. elegans DAF-16 in the regulation of lifespan
    Nataliya Libina
    Department of Biochemistry and Biophysics, Mission Bay Genentech Hall, 600 16th Street, Room S312D, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell 115:489-502. 2003
    ..We suggest that this network of tissue interactions and feedback regulation allows the tissues to equilibrate and fine-tune their expression of downstream genes, which, in turn, coordinates their rates of aging within the animal...
  61. pmc Against the oxidative damage theory of aging: superoxide dismutases protect against oxidative stress but have little or no effect on life span in Caenorhabditis elegans
    Ryan Doonan
    Institute of Healthy Ageing and Research Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom
    Genes Dev 22:3236-41. 2008
    ..We tested the impact of each SOD isoform in Caenorhabditis elegans by manipulating its five sod genes and saw no major effects on life span...
  62. pmc Massively parallel sequencing of the polyadenylated transcriptome of C. elegans
    Ladeana W Hillier
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington 98195 5065, USA
    Genome Res 19:657-66. 2009
    ..sequencing by synthesis methods, we have surveyed the polyA+ transcripts from four stages of the nematode Caenorhabditis elegans to an unprecedented depth...
  63. pmc Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG
    Yingchuan B Qi
    Division of Biological Sciences, Neurobiology Section, University of California, San Diego, CA 92093, USA
    Proc Natl Acad Sci U S A 109:7499-504. 2012
    ..We transgenically expressed mitochondrially targeted miniSOG (mito-miniSOG) in Caenorhabditis elegans neurons...
  64. pmc Many families of C. elegans microRNAs are not essential for development or viability
    Ezequiel Alvarez-Saavedra
    Howard Hughes Medical Institute, Department of Biology, MIT, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Curr Biol 20:367-73. 2010
    ..Conversely, because in some cases miRNA family members act redundantly, our findings emphasize the importance of determining miRNA function in the absence of miRNAs related in sequence...
  65. pmc Cytoskeletal forces span the nuclear envelope to coordinate meiotic chromosome pairing and synapsis
    Aya Sato
    Howard Hughes Medical Institute, Chevy Chase, MD 20815 USA
    Cell 139:907-19. 2009
    ..In the nematode Caenorhabditis elegans, special regions on each chromosome known as pairing centers are essential for both homologous pairing and ..
  66. pmc Structural properties of the Caenorhabditis elegans neuronal network
    Lav R Varshney
    Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS Comput Biol 7:e1001066. 2011
    ..Even for Caenorhabditis elegans, whose neuronal network is relatively small and stereotypical from animal to animal, published wiring ..
  67. ncbi Autophagy genes are essential for dauer development and life-span extension in C. elegans
    Alicia Melendez
    Department of Medicine, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, NY 10032, USA
    Science 301:1387-91. 2003
    Both dauer formation (a stage of developmental arrest) and adult life-span in Caenorhabditis elegans are negatively regulated by insulin-like signaling, but little is known about cellular pathways that mediate these processes...
  68. ncbi R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway
    Qinghua Liu
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Science 301:1921-5. 2003
    ..R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4. Association with R2D2 does not affect the enzymatic activity of DCR-2...
  69. pmc Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans
    R F Ketting
    The Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, Utrecht, The Netherlands
    Genes Dev 15:2654-9. 2001
    ..Here we characterize the Caenorhabditis elegans ortholog of Dicer (K12H4.8; dcr-1) in vivo and in vitro. dcr-1 mutants show a defect in RNAi...
  70. pmc Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte
    B Grant
    Columbia University College of Physicians and Surgeons, Department of Biochemistry and Molecular Biophysics, New York, New York 10032, USA
    Mol Biol Cell 10:4311-26. 1999
    The Caenorhabditis elegans oocyte is a highly amenable system for forward and reverse genetic analysis of receptor-mediated endocytosis...
  71. pmc Inhibition of respiration extends C. elegans life span via reactive oxygen species that increase HIF-1 activity
    Seung Jae Lee
    Division of Molecular and Life Science, School of Interdisciplinary Bioscience and Bioengineering, and World Class University Information Technology Convergence Engineering, Pohang University of Science and Technology, Pohang, Kyungbuk 790 784, South Korea
    Curr Biol 20:2131-6. 2010
    ..In this way, HIF-1 appears to link respiratory stress in the mitochondria to a nuclear transcriptional response that promotes longevity...
  72. ncbi CED-1 is a transmembrane receptor that mediates cell corpse engulfment in C. elegans
    Z Zhou
    Howard Hughes Medical Institute, Department of Biology, Building 68, Room 425, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Cell 104:43-56. 2001
    ..We suggest that the ABC transporter CED-7 promotes cell corpse recognition by CED-1, possibly by exposing a phospholipid ligand on the surfaces of cell corpses...
  73. pmc The DNA-encoded nucleosome organization of a eukaryotic genome
    Noam Kaplan
    Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel
    Nature 458:362-6. 2009
    ..of nucleosome sequence preferences that is significantly correlated with in vivo nucleosome occupancy in Caenorhabditis elegans. Our results indicate that the intrinsic DNA sequence preferences of nucleosomes have a central role in ..
  74. pmc Most Caenorhabditis elegans microRNAs are individually not essential for development or viability
    Eric A Miska
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Genet 3:e215. 2007
    ..We report the generation of deletion mutations in 87 miRNA genes in Caenorhabditis elegans, expanding the number of mutated miRNA genes to 95, or 83% of known C. elegans miRNAs...
  75. ncbi Genome-wide RNAi screening in Caenorhabditis elegans
    Ravi S Kamath
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Methods 30:313-21. 2003
    In Caenorhabditis elegans, introduction of double-stranded RNA (dsRNA) results in the specific inactivation of an endogenous gene with corresponding sequence; this technique is known as RNA interference (RNAi)...
  76. ncbi daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans
    S T Henderson
    Institute for Behavioral Genetics, University of Colorado at Boulder, Campus Box 447, Boulder, CO 80309 0447, USA
    Curr Biol 11:1975-80. 2001
    ..We suggest that changes in the subcellular localization of DAF-16 by environmental cues allows for rapid reallocation of resources in response to a changing environment at all stages of life...
  77. ncbi Impaired insulin/IGF1 signaling extends life span by promoting mitochondrial L-proline catabolism to induce a transient ROS signal
    Kim Zarse
    Department of Human Nutrition, Institute of Nutrition, University of Jena, Jena, Germany
    Cell Metab 15:451-65. 2012
    ..elegans life span. Taken together, iIIS promotes L-proline metabolism to generate a ROS signal for the adaptive induction of endogenous stress defense to extend life span...
  78. pmc A mitochondrial superoxide signal triggers increased longevity in Caenorhabditis elegans
    Wen Yang
    Department of Biology, McGill University, Montreal, Quebec, Canada
    PLoS Biol 8:e1000556. 2010
    ..This model provides a new explanation for the well-documented correlation between ROS and the aged phenotype as a gradual increase of molecular damage during aging would trigger a gradually stronger ROS response...
  79. pmc MicroRNAs both promote and antagonize longevity in C. elegans
    Alexandre de Lencastre
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511 USA
    Curr Biol 20:2159-68. 2010
    ..elegans, but the mechanisms of life-span regulation are not completely known. MicroRNAs (miRNAs) regulate various aspects of development and metabolism, and one miRNA has been previously implicated in life span...
  80. ncbi Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling
    K Lin
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA
    Nat Genet 28:139-45. 2001
    The lifespan of Caenorhabditis elegans is regulated by the insulin/insulin-like growth factor (IGF)-1 receptor homolog DAF-2, which signals through a conserved phosphatidylinositol 3-kinase (PI 3-kinase)/Akt pathway...
  81. pmc Motifs in brain networks
    Olaf Sporns
    Department of Psychology and Program in Cognitive Science, Indiana University, Bloomington, Indiana, USA
    PLoS Biol 2:e369. 2004
    ..These results are consistent with the hypothesis that highly evolved neural architectures are organized to maximize functional repertoires and to support highly efficient integration of information...
  82. pmc Expression pattern and, surprisingly, gene length shape codon usage in Caenorhabditis, Drosophila, and Arabidopsis
    L Duret
    Laboratoire de Biometrie, Génétique et Biologie des Populations, Unité Mixte de Recherche Centre National de la Recherche Scientifique 5558, Villeurbanne Cedex, France
    Proc Natl Acad Sci U S A 96:4482-7. 1999
    ..the expression pattern and analyzed codon usage in 8,133, 1,550, and 2,917 genes, respectively, from Caenorhabditis elegans, Drosophila melanogaster, and Arabidopsis thaliana...
  83. pmc Lifespan regulation by evolutionarily conserved genes essential for viability
    Sean P Curran
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
    PLoS Genet 3:e56. 2007
    ..We screened 2,700 genes essential for Caenorhabditis elegans development and identified 64 genes that extend lifespan when inactivated postdevelopmentally...
  84. pmc The threshold for polyglutamine-expansion protein aggregation and cellular toxicity is dynamic and influenced by aging in Caenorhabditis elegans
    James F Morley
    Department of Biochemistry, Molecular Biology, and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 99:10417-22. 2002
    ..of fluorescently tagged polyQ proteins (Q29, Q33, Q35, Q40, and Q44) in the body wall muscle cells of Caenorhabditis elegans and show that young adults exhibit a sharp boundary at 35-40 glutamines associated with the appearance of ..
  85. pmc Deletion of the mitochondrial superoxide dismutase sod-2 extends lifespan in Caenorhabditis elegans
    Jeremy M Van Raamsdonk
    Department of Biology, McGill University, Montreal, Quebec, Canada
    PLoS Genet 5:e1000361. 2009
    ..In this experiment, we examine the effect of eliminating each of the five individual sod genes present in Caenorhabditis elegans. In contrast to what is observed in other model organisms, none of the sod deletion mutants shows ..
  86. ncbi The daf-2 gene network for longevity regulates oxidative stress resistance and Mn-superoxide dismutase gene expression in Caenorhabditis elegans
    Y Honda
    Tokyo Metropolitan Institute of Gerontology, Tokyo 173 0015, Japan
    FASEB J 13:1385-93. 1999
    Longevity is regulated by the daf-2 gene network in Caenorhabditis elegans. Mutations in the daf-2 gene, which encodes a member of the insulin receptor family, confer the life extension (Age) phenotype and the constitutive dauer (a growth-..
  87. pmc Amyloid-binding compounds maintain protein homeostasis during ageing and extend lifespan
    Silvestre Alavez
    Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, California 94945, USA
    Nature 472:226-9. 2011
    ..Here we show that exposure of adult Caenorhabditis elegans to the amyloid-binding dye Thioflavin T (ThT) resulted in a profoundly extended lifespan and slowed ageing...
  88. pmc Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans
    Dimitrios G Zisoulis
    Department of Biology, Stem Cell Program, University of California, San Diego, La Jolla, California, USA
    Nat Struct Mol Biol 17:173-9. 2010
    ..Furthermore, our analyses revealed a striking enrichment of Argonaute binding sites in genes important for miRNA function, suggesting an autoregulatory role that may confer robustness to the miRNA pathway...
  89. ncbi Antioxidant defense and aging in C. elegans: is the oxidative damage theory of aging wrong?
    David Gems
    Institute of Healthy Ageing and Research Department of Genetics, Evolution and Environment, University College London, London, UK
    Cell Cycle 8:1681-7. 2009
    ..New challenges to the theory from work using nematode worms seem set to bring them down to peck at its bones. But is the theory really dead, or does it just need to be modified?..
  90. pmc Gene finding in novel genomes
    Ian Korf
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    BMC Bioinformatics 5:59. 2004
    ..Computational gene prediction continues to be an important problem, especially for genomes with little experimental data...
  91. pmc Different dietary restriction regimens extend lifespan by both independent and overlapping genetic pathways in C. elegans
    Eric L Greer
    Department of Genetics, Stanford University, Stanford, CA 94305, USA
    Aging Cell 8:113-27. 2009
    ..Here we examine the genetic pathways that mediate longevity by different DR regimens in Caenorhabditis elegans. We have previously shown that the low-energy sensing AMP-activated protein kinase AMPK/aak-2 and the ..
  92. ncbi A genome-wide map of conserved microRNA targets in C. elegans
    Sabbi Lall
    Center for Comparative Functional Genomics, Department of Biology, New York University, New York, New York 10003, USA
    Curr Biol 16:460-71. 2006
    ..Metazoan miRNAs regulate protein-coding genes by binding the 3' UTR of cognate mRNAs. Identifying targets for the 115 known C. elegans miRNAs is essential for understanding their function...
  93. pmc Alpha-synuclein is part of a diverse and highly conserved interaction network that includes PARK9 and manganese toxicity
    Aaron D Gitler
    Whitehead Institute for Biomedical Research and Howard Hughes Medical Institute, Cambridge, Massachusetts 02142, USA
    Nat Genet 41:308-15. 2009
    ..Further, knockdown of the ATP13A2 ortholog in Caenorhabditis elegans enhances alpha-syn misfolding...
  94. pmc A comprehensive analysis of gene expression changes provoked by bacterial and fungal infection in C. elegans
    Ilka Engelmann
    Centre d immunologie de Marseille Luminy, Universite de la Mediterranee, Marseille, France
    PLoS ONE 6:e19055. 2011
    While Caenorhabditis elegans specifically responds to infection by the up-regulation of certain genes, distinct pathogens trigger the expression of a common set of genes...
  95. ncbi Two neurons mediate diet-restriction-induced longevity in C. elegans
    Nicholas A Bishop
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 447:545-9. 2007
    ..Here we show that increased longevity of diet-restricted Caenorhabditis elegans requires the transcription factor gene skn-1 acting in the ASIs, a pair of neurons in the head...
  96. pmc Parallel evolution of domesticated Caenorhabditis species targets pheromone receptor genes
    Patrick T McGrath
    Howard Hughes Medical Institute, Laboratory of Neural Circuits and Behavior, The Rockefeller University, New York, New York 10065, USA
    Nature 477:321-5. 2011
    ..Two strains of Caenorhabditis elegans grown at high density have independently acquired multigenic resistance to pheromone-induced dauer ..
  97. ncbi Long-lived C. elegans daf-2 mutants are resistant to bacterial pathogens
    Danielle A Garsin
    Department of Genetics, Massachusetts General Hospital, Boston, MA 02114, USA
    Science 300:1921. 2003
  98. pmc Inactivation of conserved C. elegans genes engages pathogen- and xenobiotic-associated defenses
    Justine A Melo
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Cell 149:452-66. 2012
    ..Variation in cellular surveillance and endocrine pathways controlling behavior, detoxification, and immunity selected by past toxin or microbial interactions could underlie aberrant responses to foods, medicines, and microbes...
  99. pmc Widespread protein aggregation as an inherent part of aging in C. elegans
    Della C David
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America
    PLoS Biol 8:e1000450. 2010
    ..we identified several hundred proteins that become more insoluble with age in the multicellular organism Caenorhabditis elegans. These proteins are predicted to be significantly enriched in beta-sheets, which promote disease protein ..
  100. pmc Caenorhabditis elegans HCF-1 functions in longevity maintenance as a DAF-16 regulator
    Ji Li
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA
    PLoS Biol 6:e233. 2008
    ..We report that the Caenorhabditis elegans homolog of host cell factor 1 (HCF-1) represents a new longevity modulator and functions as a negative ..
  101. pmc Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans
    Colin C Conine
    The Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01606, USA
    Proc Natl Acad Sci U S A 107:3588-93. 2010
    Gametogenesis is a thermosensitive process in numerous metazoans, ranging from worms to man. In Caenorhabditis elegans, a variety of RNA-binding proteins that associate with germ-line nuage (P granules), including the Piwi-clade argonaute ..

Research Grants79

  1. Mechanisms of Heme Transport in Macrophages
    CARINE CARINE WHITE; Fiscal Year: 2012
    ..has identified the first eukaryotic heme importer/transporter HRG-1, using the genetic animal model Caenorhabditis elegans (Nature 2008)...
  2. Neuroimaging Assessments of Brain Integrity in Aging Mice
    Ai Ling Lin; Fiscal Year: 2013
    ..well-studied one for various model organisms of extended longevity, including Saccharomyces cerevisiae, Caenorhabditis elegans, rodents and monkeys...
  3. Diverse Roles for RIC8 During G Protein Signaling in Fungi
    Katherine A Borkovich; Fiscal Year: 2012
    ..RIC8 is required for asymmetric cell division in both Caenorhabditis elegans and Drosophila...
  4. CONTROL OF EUCARYOTIC FUNCTION BY METHYLATION
    Steven G Clarke; Fiscal Year: 2013
    ..Here, we will utilize both mouse and worm (Caenorhabditis elegans) systems to explore the relationships between the accumulation of age-damaged proteins, their recognition ..
  5. Chromosomal Control of Segregation of C. Elegans
    Abby F Dernburg; Fiscal Year: 2013
    ..We study meiosis in the nematode roundworm Caenorhabditis elegans, which offers major experimental advantages, including outstanding cytology and powerful genetic tools...
  6. Microfluidic-microtiter interface for rapid large-scale screenings of C. elegans
    Adela Ben Yakar; Fiscal Year: 2010
    ..Among these animals, the nematode Caenorhabditis elegans (C. elegans) is one of the most powerful model organisms providing a wide-range of genetic tools...
  7. Presynaptic Mechanisms Regulating the Dopamine Transporter
    JAMES A HARDAWAY; Fiscal Year: 2013
    ..In the nematode Caenorhabditis elegans, synaptic spillover of DA produced by pharmacological or genetic loss of DAT (DAT-1) induces a locomotory ..
  8. Development and Regulation of Cholinergic Synapses
    Andres Villu Maricq; Fiscal Year: 2013
    ..is to gain a mechanistic understanding of AChR-mediated synaptic signaling by using a genetic approach in Caenorhabditis elegans to identify signaling pathways that contribute to the delivery or function of a7-like AChRs...
  9. Obesity and diabetes genetics in Caenorhabditis elegans.
    Alexander A Soukas; Fiscal Year: 2013
    ..Discovery of obesity and diabetes candidate genes will help both to inform future study of metabolic diseases and to design intelligent therapies to combat these devastating diseases. ..
  10. Insulin, IGF and Insulin Signaling: effects on Anopheles lifespan and immunity
    MICHAEL ALLEN RIEHLE; Fiscal Year: 2012
    ..the ISC and its impacts on mosquito physiology and vector competence that were conceptualized for Caenorhabditis elegans, Drosophila melanogaster and mammals...
  11. CONTROL OF MEIOSIS AND GERMLINE PROLIFERATION
    DAVID IRWIN GREENSTEIN; Fiscal Year: 2012
    ..The nematode Caenorhabditis elegans has emerged as a paradigm for studying meiosis and germline proliferation and their regulation by ..
  12. The Transport of Nutritional Heme in Animal Development
    Iqbal Hamza; Fiscal Year: 2013
    ..We have demonstrated that the roundworm Caenorhabditis elegans is an excellent animal model to identify heme transport pathways because it synthesizes a large number of ..
  13. Exploration of the functions of the ciliopathy Arls in cilia.
    Jinghua Hu; Fiscal Year: 2013
    ..The highly conserved ciliopathy candidates, ciliogenesis pathway, and cilia sensory function make Caenorhabditis elegans a powerful model for characterizing the physiological roles of ciliopathy genes in their native cellular ..
  14. Gene Networks Regulating Muscle Protein Degradation
    Nathaniel J Szewczyk; Fiscal Year: 2010
    ..using the simplest genetically tractable organism available for studying innervated muscle, the nematode Caenorhabditis elegans. As in human muscle, proteolysis in C...
  15. Design of a high-throughput screen for chemicals that cause meiotic aneuploidy
    Patrick Allard; Fiscal Year: 2011
    ..a high-throughput screening approach that makes use of the remarkable biological features of the nematode Caenorhabditis elegans, which as a meiotic model system shows a high degree of conservation with humans...
  16. Identifying novel anti-infectives by high through-put screening in whole animals
    Frederick M Ausubel; Fiscal Year: 2013
    ..We have developed infection models in the nematode Caenorhabditis elegans that can be used to identify drugs that cure otherwise lethal infections...
  17. Investigation of Anaphase Promoting Complex function in synaptic transmission
    Jennifer Kowalski; Fiscal Year: 2012
    ..This will be done using the model nematode, Caenorhabditis elegans, which shares significant conservation of nervous system proteins and function with humans...
  18. Epigenetic control of sex chromosome behavior in meiosis.
    JoAnne Engebrecht; Fiscal Year: 2013
    ..conserved mechanisms underlying chromosome behavior during male meiosis using the metazoan animal model Caenorhabditis elegans, which is particularly amenable to genetic, cell biological and molecular approaches...
  19. Using shared mechanisms of morphogenesis to study neural tube defects
    JESSICA LEE SULLIVAN-BROWN; Fiscal Year: 2012
    ..Gastrulation in the nematode, Caenorhabditis elegans (C...
  20. Molecular Genetic Tools for Parasitic Helminths
    Edward J Pearce; Fiscal Year: 2013
    ..Nevertheless, the free-living worm Caenorhabditis elegans and planaria can be studied using sophisticated molecular tools, suggesting that development of similar ..
  21. Germline Silencing of Unpaired Chromatin
    Eleanor M Maine; Fiscal Year: 2013
    ..The proposal specifically focuses on meiotic silencing of unpaired chromatin in the nematode, Caenorhabditis elegans. Meiotic silencing is an epigenetic regulatory mechanism that has been described in mammals and nematodes ..
  22. Cellular responses to pore-forming toxins and regulation/function of the exocyst
    Anand Sitaram; Fiscal Year: 2013
    ..Our lab recently published a genome-wide RNAi screen, performed in the Caenorhabditis elegans roundworm, that strongly implicates subunits of the exocyst as important components of the cellular ..
  23. The functional role of DRH-1 in RNAi directed viral immunity
    Rui Lu; Fiscal Year: 2013
    ..DRH-1 is a putative RNA helicase conserved in both the Caenorhabditis elegans (C .elegans) nematode and mammals...
  24. Genomic Stability Functions of SMC Proteins in Germ Cells
    Raymond C Chan; Fiscal Year: 2013
    ..We are combining genetics and cytology in the roundworm Caenorhabditis elegans for the focused study of meiotic sister-chromatid recombination...
  25. Variable Dose Rate X-ray Irradiator
    Howard B Lieberman; Fiscal Year: 2010
    ..be undertaken on a wide array of model systems such as radioresistant yeast, Drosophila melanogaster and Caenorhabditis elegans, as well as mammalian cells in culture, or small rodents. Drs...
  26. A Model for Nephronophthisis in Caenorhabditis elegans
    Maureen M Barr; Fiscal Year: 2013
    ..The nematode Caenorhabditis elegans is a powerful model organism to study the roles of the nephrocystins in their native cellular environment...
  27. Lectin-Carbohydrate Interactions in the Host-Parasite System
    Xiao Qiang Yu; Fiscal Year: 2010
    ..Immulectin-2 (IML-2) directly binds to nematodes Caenorhabditis elegans and Brugia malayi, and binding of IML-2 to C. elegans stimulates melanization of the worms...
  28. Regulation of an osmosensitive CIC anion channel by Ste20 kinase signaling
    Kevin Strange; Fiscal Year: 2012
    ..We have exploited the genetic and molecular tractability of the nematode Caenorhabditis elegans to characterize the regulation and physiological roles of ClC channels that are assembled and operational ..
  29. Genetic dissection of the RNAi-mediated antiviral immunity in C. elegans
    Shou Wei Ding; Fiscal Year: 2013
    ..The proposed research will genetically dissect the immune pathway of Caenorhabditis elegans animals triggered by Flock house virus (FHV), one of the best characterized RNA viruses...
  30. The MicroRNA Pathway in Translational Regulation of Neuronal Development
    Fen Biao Gao; Fiscal Year: 2013
    ..Indeed, miRNAs have been implicated in left/right neuronal asymmetry in Caenorhabditis elegans, photoreceptor formation and early neurogenesis in Drosophila, brain morphogenesis and neurogenesis in ..
  31. Small Molecule Signaling in Caenorhabditis elegans
    FRANK CLEMENS SCHROEDER; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The nematode Caenorhabditis elegans is one of the most important model organisms for biomedical research, because of its biological tractability and because many of its physiological pathways show ..
  32. Satiety signaling in Caenorhabditis elegans
    Leon Avery; Fiscal Year: 2013
    ..by applicant): We propose to investigate the molecular mechanisms of appetite control in the nematode Caenorhabditis elegans. Just as in mammals, C elegans appetite is promoted by hunger signals and suppressed by satiety signals...
  33. Analysis of Protein Dynamics in C. elegans by mLC/MS/MS
    Michael MacCoss; Fiscal Year: 2013
    ..turnover and the importance of insulin signaling, we propose to study both these processes in the nematode Caenorhabditis elegans using quantitative proteornics...
  34. IR-LAMP: Optigenetic technology to spatially manipulate protein function in vivo
    Julie C Canman; Fiscal Year: 2011
    ..target specific sub-cellular locations and inactivate fast-acting ts alleles in embryos from the nematode Caenorhabditis elegans with high temporal and spatial resolution...
  35. Prostaglandins in C. elegans Fertilization
    Michael A Miller; Fiscal Year: 2013
    ..The objective of this application is to determine the extent to which the nematode model Caenorhabditis elegans can be used to address fundamental questions in prostaglandin biology...
  36. Innate Immune Recognition of Candida albicans Infection in a C. elegans Model
    Read Pukkila-Worley; Fiscal Year: 2013
    ..For these studies, I will use the model host Caenorhabditis elegans to determine the mechanisms by which C. albicans activates epithelial immunity...
  37. Genetic Analysis of Iron Homeostasis in C.Elegans
    ELIZABETH ANN LEIBOLD; Fiscal Year: 2010
    ..We have discovered that Caenorhabditis elegans express ferritin (CeFTN-1 and CeFTN-2), which are key iron storage proteins...
  38. Regulation of Systemic RNA Interference in the Nematode C. elegans
    JENNIFER SUNG WHANGBO; Fiscal Year: 2013
    ..The study of systemic RNAi in the model system Caenorhabditis elegans will provide important insights for the delivery of RNAi-based therapies in humans...
  39. Mechanisms of lack of senescence and inducible senescence in the genus Hydra
    Daniel E Martinez; Fiscal Year: 2013
    ..treated to eliminate the stem cells which produce gametes, we will investigate the possibility that, as in Caenorhabditis elegans and Drosophila melanogaster, germ cells in H. oligactis can reduce lifespan...
  40. Design of a high-throughput screen for chemicals that cause meiotic aneuploidy
    Patrick Allard; Fiscal Year: 2013
    ..a high-throughput screening approach that makes use of the remarkable biological features of the nematode Caenorhabditis elegans, which as a meiotic model system shows a high degree of conservation with humans...
  41. The Dosage Compensation Machinery of C. elegans
    Gyorgyi Csankovszki; Fiscal Year: 2013
    ..In the worm Caenorhabditis elegans, dosage compensation is accomplished by a condensin-like dosage compensation complex (DCC), which binds ..
  42. Control of Yeast Life Span
    Kurt W Runge; Fiscal Year: 2013
    ..that cells lacking the ATP2 gene had a short lifespan, which was surprising since RNAi experiments in Caenorhabditis elegans indicated that reducing expression of other F1F0- ATP synthase subunits extends lifespan...
  43. Architecture of a Genetic Network Governing Morphogenesis
    David H A Fitch; Fiscal Year: 2013
    ..governing the morphogenesis of a novel, simple and sexually dimorphic model structure, the tail tip of Caenorhabditis elegans. The tail tip is composed of four cells which-in males only-radically alter their shape and position at ..
  44. Heme Oxygenase: Structure, Function and Pathogenesis
    Angela Wilks; Fiscal Year: 2010
    ..ITC and NMR) and in vivo analysis of inhibitor efficacy by high- throughput MIC50 screening and the Caenorhabditis elegans host-pathogen model of bacterial infection;iv) Optimize the inhibitors by lead compound validation and ..
  45. Mutational and evolutionary impact of mitochondrial dysfunction
    SUZANNE R ESTES; Fiscal Year: 2013
    ..The main objective will be achieved using the model nematode Caenorhabditis elegans and its sister species C. briggsae...
  46. Presynaptic Regulation of C.elegans Dopamine Transporter
    Randy D Blakely; Fiscal Year: 2013
    ..to identify and characterize novel presynaptic regulators of DA signaling using the powerful model system Caenorhabditis elegans. Over the past 15 years, the Blakely lab has developed skills in the C...
  47. Can drug targets from parasitic nematodes be usefully expressed in C. elegans?
    ADRIAN JOHN WOLSTENHOLME; Fiscal Year: 2013
    ..Functional expression of parasite genes in the free-living species, Caenorhabditis elegans, has the potential to address these problems but the complexity of the drug targets may cause difficulties...
  48. Investigating Neuroprotective Targets for Parkinson Disease Associated with Autop
    Guy A Caldwell; Fiscal Year: 2011
    ..We have exploited attributes of the nematode model system, Caenorhabditis elegans, to develop assays that facilitate the screening and isolation of conserved genetic factors that mediate ..
  49. WormBase: a core data resource for C elegans and other nematodes
    PAUL WARREN STERNBERG; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Caenorhabditis elegans is a major model system for basic biological and biomedical research and the first animal for which there is a complete description of its genome, anatomy and development, and ..
  50. COPAS BIOSORT Worm Sorter
    WOLFGANG B LIEDTKE; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Caenorhabditis elegans, a multicellular roundworm of approximately 1000 differentiated cells in adult animals, offers numerous advantages, namely it has an extremely short generation time, and its ..
  51. Characterization of a novel presynaptic target for ethanol action
    GREGG W ROMAN; Fiscal Year: 2012
    ..Munc13.1 is a presynaptic active zone protein essential for neurotransmitter release in brain. In Caenorhabditis elegans, the homologous Unc13 protein is responsible for the sensitivity to volatile anesthetics...
  52. Investigation of calcium signaling in Caenorhabditis elegans
    Kenneth Norman; Fiscal Year: 2013
    ..guanine nucleotide exchange factor for the Rho Family GTPases, regulates several rhythmic behaviors in Caenorhabditis elegans by modulating calcium oscillations...
  53. Regulation of Caenorhabditis elegans DNA polymerase eta by regulated proteolysis
    Ashley B Williams; Fiscal Year: 2011
    ..HEALTH RELEVANCE: The experiments in this proposal will explore the regulation of DNA polymerase POLH-1 in Caenorhabditis elegans. Homologs of POLH-1 (DNA polymerase 7 in humans) minimizes mutations caused by UV light, thus limiting the ..
  54. Mechanisms of Sensory Modulation of Aging in Drosophila
    Scott D Pletcher; Fiscal Year: 2013
    ..Indeed, evidence from work in the nematode, Caenorhabditis elegans, and from our work in the fruit fly, Drosophila melanogaster, has established that aging is strongly ..
  55. LIPID SIGNALING IN REGULATION OF LONGEVITY
    MENG CARLA WANG; Fiscal Year: 2013
    ..During the preliminary studies, we characterized LIPL-4 as a lysosomal acid lipase in Caenorhabditis elegans. Its constitute expression in the fat storage tissue induces lipolysis as well as prolongs organism ..
  56. Erk-mediated regulation of Dicer and Drosha function in C. elegans
    Swathi Arur; Fiscal Year: 2013
    ..of ERK signaling results in defective oocyte maturation and perturbed trophoblast development, while in Caenorhabditis elegans loss of ERK function disrupts oocyte differentiation and maturation, leading to sterility or early ..
  57. Role of Oxidative Stress and Protein Transporters in Glyphosate and Mancozeb Neur
    Vanessa A Fitsanakis; Fiscal Year: 2012
    ..Using the nematode Caenorhabditis elegans (C...
  58. Identification of genetic pathways that regulate neuronal circuits in C. elegans
    SALVATORE JAMES CHERRA; Fiscal Year: 2012
    ..The roundworm, Caenorhabditis elegans, will be utilized as a model system for four main reasons: 1) its neuronal networks are formed and ..
  59. Genome-wide investigations in fungal pathogens utilizing an invertebrate model ho
    Eleftherios Mylonakis; Fiscal Year: 2010
    ..The main objective of this proposal is the development and implementation of whole animal Caenorhabditis elegans-based, genome-wide assays to study fungal virulence. The C...
  60. Force Clamp Systems for Evaluation of Mechanotransduction
    BETH L PRUITT; Fiscal Year: 2013
    ..The proposed research uses the simple roundworm, Caenorhabditis elegans, because more is understood about its sense of touch than that of any other animal. Research using C...
  61. Lifespan and healthspan regulation by hypoxic response transcription factor HIF-1
    Matt Kaeberlein; Fiscal Year: 2013
    ..the hypoxic response transcription factor, HIF-1, as a novel modifier of longevity and healthspan in Caenorhabditis elegans. In a study published in the journal Science, we showed that stabilization of HIF-1 by inhibiting its ..
  62. MicroRNA genes and their functions
    David P Bartel; Fiscal Year: 2013
    ..They will also identify long noncoding RNAs in Caenorhabditis elegans, flies, and vertebrates...
  63. Secretory Vesicle Function During Spermatogenesis & Fertilization in C. elegans
    STEVEN W L apos HERNAULT; Fiscal Year: 2010
    ..Our preliminary data show that Caenorhabditis elegans spermatogenesis is ideally suited for analyzing the V-ATPase role and regulation during biogenesis and ..
  64. The role of cannabinoid signaling in the nematode Caenorhabditis elegans
    MATTHEW SIMON GILL; Fiscal Year: 2010
    ..in many different species, including some invertebrates, but they have not been identified in the nematode Caenorhabditis elegans. In C...
  65. Compounds Targeting Nematode Secretion as Novel Anti-filarial Drugs
    Bethany Westlund; Fiscal Year: 2006
    ..Using the model nematode Caenorhabditis elegans, we have identified 30 compounds that inhibited secretion from a collection of 10,000 small molecules ..
  66. Development of a genetically tractable nematode model of alphavirus infection
    Margaret R Macdonald; Fiscal Year: 2010
    ..This project will utilize the well-studied nematode Caenorhabditis elegans as a model organism for studying the role of host factors in Alphavirus replication...
  67. GENETICS OF NEMATODE PHARYNGEAL MUSCLE EXCITABILITY
    Leon Avery; Fiscal Year: 2003
    ..molecular understanding of the function of an electrically excitable cell, the pharyngeal muscle cell of Caenorhabditis elegans. Through our past research we have developed a model describing key ion channels active at each stage of ..
  68. NR4A Regulation of Organ Morphogenesis
    CHRISTOPHER GISSENDANNER; Fiscal Year: 2009
    ..and characterize NR4A nuclear receptor pathways regulating organ morphogenesis in the model organism Caenorhabditis elegans. The NR4A nuclear receptors are a conserved, functionally diverse group of transcription factors that ..
  69. MOLECULAR GENETICS OF C. ELEGANS RNA POLYMERASE II
    DONALD RIDDLE; Fiscal Year: 1990
    The nematode, Caenorhabditis elegans, is currently utilized as a laboratory model for a variety of biomedical research applications, including the study of metazoan development...
  70. C elegans:a model to study intestinal water-soluble vitamins transport in vivo
    HAMID SAID; Fiscal Year: 2007
    ..unreadable] DESCRIPTION (provided by applicant): The aim of this R21 proposal is to establish the nematode Caenorhabditis elegans (C...
  71. Understanding the Role of MMPs in Basement Membrane Breaching In vivo
    LAURA CATHERINE KELLEY; Fiscal Year: 2012
    ..live-cell imaging with the highly- stereotyped and genetically tractable model of anchor cell invasion in Caenorhabditis elegans to uncover the molecular mechanisms regulating basement membrane breaching in vivo...
  72. A C.elegans high-throughput assay for the identification of new antifungal agents
    Eleftherios Mylonakis; Fiscal Year: 2010
    The main objective of this proposal is the development of automated, high-throughput, Caenorhabditis elegans-based assays that can be used to screen chemical compounds and identify those with antifungal activity...
  73. GENETICS OF CAENORHABDITIS ELEGANS SPERM MORPHOGENESIS
    STEVEN L HERNAULT; Fiscal Year: 2003
    ..We study the genetic control of asymmetric partitioning during Caenorhabditis elegans spermatocyte development, which offers some of the advantages of a unicellular system within a metazoan ..
  74. Identifying New Aging and Longevity Genes in Caenorhabditis elegans
    Muneesh Tewari; Fiscal Year: 2007
    ..It is anticipated that the combined approach of protein interaction mapping and RNAi-based analysis will identify many new genes involved in dauer formation, aging and longevity. [unreadable] [unreadable] [unreadable]..
  75. DEVELOPMENT GLYCOBIOLOGY OF CAENORHABDITIS ELEGANS
    Richard Cummings; Fiscal Year: 2003
    ..the opportunity to use genetic approaches to study these potential functions is the common soil nematode Caenorhabditis elegans. C...
  76. FACTORS REGULATING TRANSPOSITION IN C. ELEGANS
    John Collins; Fiscal Year: 1992
    The goal of this research is to understand transposable element activity and its control in the nematode Caenorhabditis elegans. The process of transposition is a major source of spontaneous mutation in a wide range of organisms, ..
  77. Linked Protein Repair, Proteolysis, and Oxidation in Aging
    Steven Clarke; Fiscal Year: 2009
    ..We propose to use mice, yeast (Saccharomyces cerevisiae), and nematode worms (Caenorhabditis elegans) as model systems...
  78. Novel whole-animal screens for anti-microbials
    FREDERICK AUSUBEL; Fiscal Year: 2009
    The goal of the propoposed project is to develop Caenorhabditis elegans whole-animal high-throughput screening assays in 96-well plates that lead to the identification of novel classes of antimicrobial or immune- enhancing compounds...
  79. COPAS SELECT Embryo Sorter
    Deborah Andrew; Fiscal Year: 2009
    ..a COPAS Select Embryo Sorter for use in research and education in studies of Drosophila melanogaster and Caenorhabditis elegans. Historically, these organisms have had a significant impact on our understanding of human biology;a major ..