sea urchins

Summary

Summary: Somewhat flattened, globular echinoderms, having thin, brittle shells of calcareous plates. They are useful models for studying FERTILIZATION and EMBRYO DEVELOPMENT.

Top Publications

  1. pmc All males are not created equal: fertility differences depend on gamete recognition polymorphisms in sea urchins
    S R Palumbi
    Department of Organismic Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 96:12632-7. 1999
  2. ncbi Molecular identification of a hyperpolarization-activated channel in sea urchin sperm
    R Gauss
    Institut für Biologische Informationsverarbeitung, Forschungszentrum Julich, Germany
    Nature 393:583-7. 1998
  3. pmc Evolutionary plasticity of developmental gene regulatory network architecture
    Veronica F Hinman
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Proc Natl Acad Sci U S A 104:19404-9. 2007
  4. pmc Evolutionary crossroads in developmental biology: sea urchins
    David R McClay
    Department of Biology, Duke University, Durham, NC 27708, USA
    Development 138:2639-48. 2011
  5. pmc SpBase: the sea urchin genome database and web site
    R Andrew Cameron
    Center for Computational Regulatory Genomics, Beckman Institute 139 74, California Institute of Technology, Pasadena, CA 91104, USA
    Nucleic Acids Res 37:D750-4. 2009
  6. ncbi Voltage-sensing mechanism is conserved among ion channels gated by opposite voltages
    Roope Mannikko
    Department of Neuroscience, The Nobel Institute for Neurophysiology, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Nature 419:837-41. 2002
  7. ncbi A genomic regulatory network for development
    Eric H Davidson
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 295:1669-78. 2002
  8. pmc Developmental gene regulatory network architecture across 500 million years of echinoderm evolution
    Veronica F Hinman
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 100:13356-61. 2003
  9. ncbi Sea urchin Forkhead gene family: phylogeny and embryonic expression
    Qiang Tu
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Dev Biol 300:49-62. 2006
  10. pmc Individual variability in reproductive success determines winners and losers under ocean acidification: a case study with sea urchins
    Peter Schlegel
    Department of Biological Sciences, Macquarie University, Sydney, Australia
    PLoS ONE 7:e53118. 2012

Detail Information

Publications302 found, 100 shown here

  1. pmc All males are not created equal: fertility differences depend on gamete recognition polymorphisms in sea urchins
    S R Palumbi
    Department of Organismic Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 96:12632-7. 1999
    ..They also suggest that positive selection at gamete recognition loci like bindin involves strong selection within species on mate choice interactions...
  2. ncbi Molecular identification of a hyperpolarization-activated channel in sea urchin sperm
    R Gauss
    Institut für Biologische Informationsverarbeitung, Forschungszentrum Julich, Germany
    Nature 393:583-7. 1998
    ..Because of their sequence and functional properties, Ih channels form a class of their own within the superfamily of voltage-gated and cyclic-nucleotide-gated channels...
  3. pmc Evolutionary plasticity of developmental gene regulatory network architecture
    Veronica F Hinman
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Proc Natl Acad Sci U S A 104:19404-9. 2007
    Sea stars and sea urchins evolved from a last common ancestor that lived at the end of the Cambrian, approximately half a billion years ago...
  4. pmc Evolutionary crossroads in developmental biology: sea urchins
    David R McClay
    Department of Biology, Duke University, Durham, NC 27708, USA
    Development 138:2639-48. 2011
    Embryos of the echinoderms, especially those of sea urchins and sea stars, have been studied as model organisms for over 100 years...
  5. pmc SpBase: the sea urchin genome database and web site
    R Andrew Cameron
    Center for Computational Regulatory Genomics, Beckman Institute 139 74, California Institute of Technology, Pasadena, CA 91104, USA
    Nucleic Acids Res 37:D750-4. 2009
    SpBase is a system of databases focused on the genomic information from sea urchins and related echinoderms. It is exposed to the public through a web site served with open source software (http://spbase.org/)...
  6. ncbi Voltage-sensing mechanism is conserved among ion channels gated by opposite voltages
    Roope Mannikko
    Department of Neuroscience, The Nobel Institute for Neurophysiology, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Nature 419:837-41. 2002
    ....
  7. ncbi A genomic regulatory network for development
    Eric H Davidson
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 295:1669-78. 2002
    ..Its architecture reveals specific and general aspects of development, such as how given cells generate their ordained fates in the embryo and why the process moves inexorably forward in developmental time...
  8. pmc Developmental gene regulatory network architecture across 500 million years of echinoderm evolution
    Veronica F Hinman
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 100:13356-61. 2003
    ..Their endomesodermal fate maps are similar, except that sea urchins generate a skeletogenic cell lineage that produces a prominent skeleton lacking entirely in starfish larvae...
  9. ncbi Sea urchin Forkhead gene family: phylogeny and embryonic expression
    Qiang Tu
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Dev Biol 300:49-62. 2006
    ..The sea urchin fox genes clearly execute many different regulatory functions, and almost all of them participate in the process of embryonic development...
  10. pmc Individual variability in reproductive success determines winners and losers under ocean acidification: a case study with sea urchins
    Peter Schlegel
    Department of Biological Sciences, Macquarie University, Sydney, Australia
    PLoS ONE 7:e53118. 2012
    ..Consequently, the extent to which inter-individual variation mediates different selection responses has not been tested. Investigating this variation is important, since some individuals may be preadapted to future climate scenarios...
  11. ncbi Mechanism regulating Ca2+-dependent mechanosensory behaviour in sea urchin spermatozoa
    Yuka Kambara
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan
    Cell Struct Funct 36:69-82. 2011
    ..The results show that the Ca(2+)-dependent mechanosensory behaviour of the sea urchin sperm is regulated by organized functioning of the membrane environment including the plasma membrane proteins and flagellasialin...
  12. ncbi The sea urchin's siren
    Thoru Pederson
    Department of Biochemistry and Molecular Pharmacology, Program in Cell Dynamics, University of Massachusetts Medical School, 377 Plantation Street, Worcester, MA 01605, USA
    Dev Biol 300:9-14. 2006
    ..Like all of us, I am excited to see the S. purpuratus genome appear and heartily congratulate, by writing this essay, the trailblazers whose intellectual courage and persistence has brought us to this happy position...
  13. ncbi Nodal and BMP2/4 pattern the mesoderm and endoderm during development of the sea urchin embryo
    Veronique Duboc
    UPMC Univ Paris 06 CNRS, UMR 7009 Biologie du Développement Observatoire Océanologique, 06230 Villefranche sur Mer, France
    Development 137:223-35. 2010
    ....
  14. ncbi Sulfated fucans from the egg jellies of the closely related sea urchins Strongylocentrotus droebachiensis and Strongylocentrotus pallidus ensure species-specific fertilization
    Ana Cristina E S Vilela-Silva
    Laboratório de Tecido Conjuntivo, Hospital Universitario Clementino Fraga Filho, and the Departamento de Bioquimica Médica, Centro de Ciencias da Saude, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941 590, Brazil
    J Biol Chem 277:379-87. 2002
    ..Sulfated polysaccharides from egg jelly are the molecules responsible for inducing the sperm acrosome reaction in sea urchins. This is an obligatory event for sperm binding to, and fusion with, the egg...
  15. ncbi LvNotch signaling mediates secondary mesenchyme specification in the sea urchin embryo
    D R Sherwood
    Developmental, Cell and Molecular Biology Group, Box 91000, Duke University, Durham, NC 27708, USA
    Development 126:1703-13. 1999
    ..Taken together, these results offer compelling evidence that LvNotch signaling directly specifies the SMC fate, and that this signaling is critical for the differential specification of SMCs and endoderm in the sea urchin embryo...
  16. ncbi Caught in the evolutionary act: precise cis-regulatory basis of difference in the organization of gene networks of sea stars and sea urchins
    Veronica F Hinman
    Department of Biological Sciences, Carnegie Mellon University, PA 15213, USA
    Dev Biol 312:584-95. 2007
    ..Finally, inter-specific gene transfer experiments confirm this scenario and demonstrate evolution occurring at the level of sequence changes to the cis-regulatory module...
  17. pmc Sea urchins predation facilitates coral invasion in a marine reserve
    Rafel Coma
    Centre d Estudis Avançats de Blanes, Consejo Superior de Investigaciones Cientificas, Blanes, Spain
    PLoS ONE 6:e22017. 2011
    ..b>Sea urchins overgraze macroalgae and create barren patches in the space-limited macroalgal community that subsequently ..
  18. ncbi Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins
    Gaia Pigino
    Biomolecular Research Laboratory, Paul Scherrer Institute, Switzerland
    J Struct Biol 178:199-206. 2012
    ..This comparative structural analysis defines the diversity of molecular architectures in these organisms, and forms the basis for future correlation with their different bending-motions...
  19. pmc Hysteresis in the voltage dependence of HCN channels: conversion between two modes affects pacemaker properties
    Roope Mannikko
    Department of Neuroscience, The Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden
    J Gen Physiol 125:305-26. 2005
    ..Computer simulations suggest that voltage hysteresis in HCN channels decreases the risk of arrhythmia in pacemaker cells...
  20. ncbi Divergent patterns of neural development in larval echinoids and asteroids
    Yoko Nakajima
    Department of Biology, Keio University, Yokohama 223 8521, Japan
    Evol Dev 6:95-104. 2004
    ..Although there are several shared features of the larval nervous systems of echinoids and asteroids, the patterns of development reveal fundamental differences in neural ontogeny...
  21. ncbi A marine diatom-derived aldehyde induces apoptosis in copepod and sea urchin embryos
    Giovanna Romano
    Stazione Zoologica Anton Dohrn Villa Comunale, I 80121 Naples, Italy
    J Exp Biol 206:3487-94. 2003
    ..Since diatoms are an important food source for marine herbivores such as copepods and sea urchins, these findings may help explain why unsaturated aldehydes often induce reproductive failure, with important ..
  22. pmc A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory networks
    Albert J Poustka
    Max Planck Institut fur Molekulare Genetik, Evolution and Development Group, Ihnestrasse 73, 14195 Berlin, Germany
    Genome Biol 8:R85. 2007
    ..The genome of the sea urchin Strongylocentrotus purpuratus has recently been sequenced because it is a major model system for the study of gene regulatory networks. Embryonic expression patterns for most genes are unknown, however...
  23. ncbi The molecular architecture of axonemes revealed by cryoelectron tomography
    Daniela Nicastro
    Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, CB 347, University of Colorado, Boulder, CO 80309 0347, USA
    Science 313:944-8. 2006
    ..Periodic densities were also observed inside doublet microtubules; these may contribute to doublet stability...
  24. ncbi The molecular evolution of sperm bindin in six species of sea urchins (Echinoida: Strongylocentrotidae)
    C H Biermann
    Department of Ecology and Evolution, State University of New York at Stony Brook, USA
    Mol Biol Evol 15:1761-71. 1998
    ..A large proportion of the bindin-coding sequence consists of a highly variable repeat region. Bindin sequences, even including the large intron, could not resolve the branching order among five of the species...
  25. pmc Evolutionary animation: how do molecular phylogenies compare to Mayr's reconstruction of speciation patterns in the sea?
    Stephen R Palumbi
    Hopkins Marine Station, Department of Biological Sciences, Stanford University, Pacific Grove, CA 93950, USA
    Proc Natl Acad Sci U S A 102:6566-72. 2005
    ..applied to a wide set of taxa, and a seminal paper by Mayr used it to explore speciation patterns in tropical sea urchins. Since then, taxonomic information in several of these genera has been augmented by detailed molecular ..
  26. pmc ISWI contributes to ArsI insulator function in development of the sea urchin
    Mamiko Yajima
    MCB Department, Brown University, 185 Meeting Street, BOX GL173, Providence, RI 02912, USA
    Development 139:3613-22. 2012
    ..These studies reveal a mechanistic basis for ArsI function in the gene regulatory network of early development...
  27. pmc Dynamic expression of multiple scavenger receptor cysteine-rich genes in coelomocytes of the purple sea urchin
    Z Pancer
    Division of Biology 156 29, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 97:13156-61. 2000
    ..The mechanisms controlling SRCR gene expression and the functional significance of this dynamic system await elucidation...
  28. ncbi Pattern formation in a pentameral animal: induction of early adult rudiment development in sea urchins
    Sharon B Minsuk
    Department of Biology and Indiana Molecular Biology Institute, Indiana University, Bloomington, Indiana 47405, USA
    Dev Biol 247:335-50. 2002
    ..We compare HeARS expression in H. erythrogramma with that in indirect developers and discuss its implications for modularity in the evolution of developmental mode...
  29. ncbi Morphological evolution of newly metamorphosed sea urchins--a phylogenetic and functional analysis
    Richard B Emlet
    Oregon Institute of Marine Biology and Department of Biology, University of Oregon, Charleston, OR 97420, USA
    Integr Comp Biol 50:571-88. 2010
    Newly metamorphosed juvenile sea urchins are highly variable across taxa...
  30. pmc Atypical protein kinase C controls sea urchin ciliogenesis
    Gerard Pruliere
    Observatoire Oceanologique, Biologie du Développement, Universite Pierre et Marie Curie and CNRS, Villefranche sur Mer, France
    Mol Biol Cell 22:2042-53. 2011
    ..We suggest that aPKC might function to phosphorylate kinesin and so activate the transport of intraflagellar vesicles...
  31. ncbi Sea urchin fertilization in a warm, acidified and high pCO2 ocean across a range of sperm densities
    Maria Byrne
    Schools of Medical and Biological Sciences, University of Sydney, NSW 2006, Australia
    Mar Environ Res 69:234-9. 2010
    ..It is important to identify where vulnerabilities lie across life histories and our results indicate that sea urchin fertilization is robust to climate change stressors. However, developmental stages may be vulnerable to ocean change...
  32. pmc Uncoupling of complex regulatory patterning during evolution of larval development in echinoderms
    Kristen A Yankura
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    BMC Biol 8:143. 2010
    ..The embryos of these animals are microscopic, feeding within the plankton until they metamorphose into their adult forms...
  33. pmc The rate of change in Ca(2+) concentration controls sperm chemotaxis
    Luis Alvarez
    Department of Molecular Sensory Systems, Center of Advanced European Studies and Research caesar, 53175 Bonn, Germany
    J Cell Biol 196:653-63. 2012
    ..The cytoskeleton of cilia, the axoneme, is highly conserved. Thus, motile ciliated cells in general might use a similar cellular computation to translate changes of [Ca(2+)](i) into motion...
  34. ncbi Offerings from an urchin
    Susan G Ernst
    Department of Biology, Tufts University, Medford, MA 02155, USA
    Dev Biol 358:285-94. 2011
    ....
  35. ncbi A Wnt-FoxQ2-nodal pathway links primary and secondary axis specification in sea urchin embryos
    Shunsuke Yaguchi
    National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 14:97-107. 2008
    ..Therefore, restriction of FoxQ2 to the animal plate is a crucial element of canonical Wnt signaling that coordinates patterning along the AV axis with the initiation of OA specification...
  36. ncbi Conspecific sperm precedence in two species of tropical sea urchins
    Laura B Geyer
    Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Evolution 59:97-105. 2005
    ....
  37. ncbi Rapid microinjection of fertilized eggs
    Melani S Cheers
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Methods Cell Biol 74:287-310. 2004
  38. ncbi Effects of anthropogenic seawater acidification on acid-base balance in the sea urchin Psammechinus miliaris
    Hayley Miles
    Marine Biology and Ecology Research Centre, School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, Devon PL4 8AA, UK
    Mar Pollut Bull 54:89-96. 2007
    ..Geological sequestration leaks may result in dramatic localised pH reductions, e.g. pH 5.8. P. miliaris is intolerant of pH 6.16 seawater and significant mortality is seen at pH 6.63...
  39. ncbi Molecular characterisation of SALMFamide neuropeptides in sea urchins
    Maurice R Elphick
    School of Biological and Chemical Sciences, Queen Mary, University of London, UK
    J Exp Biol 208:4273-82. 2005
    ..Our aim here was to characterise SALMFamide neuropeptides in sea urchins (class Echinoidea)...
  40. pmc Gene regulatory network subcircuit controlling a dynamic spatial pattern of signaling in the sea urchin embryo
    Joel Smith
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 105:20089-94. 2008
    ..Thus the specific cis-regulatory linkages of the gene regulatory network encode the coordinated spatial expression of Wnt and Notch signaling as they sweep outward across the vegetal plate of the embryo...
  41. ncbi The dynamic gene expression patterns of transcription factors constituting the sea urchin aboral ectoderm gene regulatory network
    Jen Hao Chen
    Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan
    Dev Dyn 240:250-60. 2011
    ..The dynamic changes in the expression patterns of these transcription factor genes and the nuclearization of pSmad1/5/8 may provide a foundation for resolving the AE GRN...
  42. pmc The sea urchin sperm receptor for egg jelly is a modular protein with extensive homology to the human polycystic kidney disease protein, PKD1
    G W Moy
    Marine Biology Research Division, University of California, San Diego, La Jolla 92093 0202, USA
    J Cell Biol 133:809-17. 1996
    ..The lesion in cellular physiology resulting from mutations in the PKD1 protein remains unknown. The homology between REJ modules of the sea urchin REJ and human PKD1 suggests that PKD1 could be involved in ionic regulation...
  43. ncbi Total synthesis of cyclic ADP-carbocyclic-ribose, a stable mimic of Ca2+-mobilizing second messenger cyclic ADP-ribose
    S Shuto
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    J Am Chem Soc 123:8750-9. 2001
    ..When cADPcR was injected into sea urchin eggs, it caused a significant release of Ca2+ in the cells, an effect considerably stronger than that of cADPR. Thus, cADPcR was identified as a stable mimic of cADPR...
  44. doi Near-future levels of ocean acidification reduce fertilization success in a sea urchin
    Jon N Havenhand
    Curr Biol 18:R651-R652. 2008
  45. ncbi The relationship between conspecific fertilization success and reproductive isolation among three congeneric sea urchins
    Don R Levitan
    Department of Biological Science, Florida State University, Tallahassee 32306 1100, USA
    Evolution 56:1599-609. 2002
    ..Field observations indicate that S. droebachiensis is often surrounded by heterospecific sea urchins. Genetic analysis of larvae produced during heterospecific spawning events indicate that hybrids are generally ..
  46. ncbi Superfamilies of evolved and designed networks
    Ron Milo
    Departments of Molecular Cell Biology, Physics of Complex Systems, and Computer Science, Weizmann Institute of Science, Rehovot 76100, Israel
    Science 303:1538-42. 2004
    ..Additional superfamilies include power grids, protein-structure networks and geometric networks, World Wide Web links and social networks, and word-adjacency networks from different languages...
  47. ncbi Speciation on the coasts of the new world: phylogeography and the evolution of bindin in the sea urchin genus Lytechinus
    Kirk S Zigler
    Smithsonian Tropical Research Institute, Box 2072, Balboa, Panama
    Evolution 58:1225-41. 2004
    Beginning with E. Mayr's study in 1954, tropical sea urchins have played an important role in studies of speciation in the sea, but what are the processes of cladogenesis and divergence that give rise to new species in this group? We ..
  48. pmc Vasa protein expression is restricted to the small micromeres of the sea urchin, but is inducible in other lineages early in development
    Ekaterina Voronina
    Providence Institute of Molecular Oogenesis, Department of Molecular Biology, Cell Biology and Biochemistry, 185 Meeting Street, Box G, Brown University, Providence, RI 02912, USA
    Dev Biol 314:276-86. 2008
    ..Overall, these results support the contention that sea urchins do not have obligate primordial germ cells determined in early development, that vasa may function in an early ..
  49. ncbi R11: a cis-regulatory node of the sea urchin embryo gene network that controls early expression of SpDelta in micromeres
    Roger Revilla-i-Domingo
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Dev Biol 274:438-51. 2004
    ..In addition, we introduce new methodological tools for quantitative measurement of the output of expression constructs that promise to be of general value for cis-regulatory analysis in sea urchin embryos...
  50. ncbi The role of micromere signaling in Notch activation and mesoderm specification during sea urchin embryogenesis
    H C Sweet
    Department of Biological Sciences and Science and Technology Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Development 126:5255-65. 1999
    ..We propose that the micromeres induce adjacent cells to form SMCs, possibly by presenting a ligand for the Notch receptor...
  51. ncbi Marine reserves reestablish lost predatory interactions and cause community changes in rocky reefs
    Paolo Guidetti
    Laboratory of Zoology and Marine Biology, DiSTeBA, University of Lecce, via prov le Monteroni, 73100 Lecce, Italy
    Ecol Appl 16:963-76. 2006
    ..Protected locations supported higher density and size of the most effective fish preying on sea urchins (the sea breams Diplodus sargus and D. vulgaris) than unprotected locations...
  52. pmc Functional cis-regulatory genomics for systems biology
    Jongmin Nam
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 107:3930-5. 2010
    ..This approach will qualitatively alter the practice of GRN construction as well as validation, and will impact many additional areas of regulatory system biology...
  53. ncbi Tuning sperm chemotaxis by calcium burst timing
    Adan Guerrero
    Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
    Dev Biol 344:52-65. 2010
    ..Tuning of Ca(2+) fluctuations and associated turning episodes to the chemoattractant gradient polarity is a central feature of sea urchin sperm chemotaxis and may be a feature of sperm chemotaxis in general...
  54. pmc Impact of ocean warming and ocean acidification on larval development and calcification in the sea urchin Tripneustes gratilla
    Hannah Sheppard Brennand
    School of Medical Sciences, University of Sydney, Sydney, New South Whales, Australia
    PLoS ONE 5:e11372. 2010
    ..However, this may be buffered by enhanced growth and metabolism due to warming...
  55. ncbi An atypical CNG channel activated by a single cGMP molecule controls sperm chemotaxis
    Wolfgang Bönigk
    Center of Advanced European Studies and Research, Abteilung Molekulare Neurosensorik, Ludwig Erhard Allee 2, 53175 Bonn, Germany
    Sci Signal 2:ra68. 2009
    ..Thus, CNGK has developed a mechanism of activation that is different from the activation of other CNG channels, which requires the cooperative binding of several ligands and operates in the micromolar rather than the nanomolar range...
  56. pmc Nanos functions to maintain the fate of the small micromere lineage in the sea urchin embryo
    Celina E Juliano
    Department of Molecular and Cell Biology and Biochemistry, Brown University, 185 Meeting St, Providence, RI 02912, USA
    Dev Biol 337:220-32. 2010
    ..This work, in combination with other recent results in Ilyanassa and Platynereis dumerilii, suggests the presence of a conserved molecular program underlying both primordial germ cell and multipotent cell specification and maintenance...
  57. ncbi Specification of cell fate in the sea urchin embryo: summary and some proposed mechanisms
    E H Davidson
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Development 125:3269-90. 1998
    ..The requirements for postembryonic adult body plan formation in the larval rudiment include engagement of a new level of genetic regulatory apparatus, exemplified by the Hox gene complex...
  58. ncbi Heterodimerization of the two motor subunits of the heterotrimeric kinesin, KRP85/95
    D J Rashid
    Section of Molecular and Cellular Biology, University of California, Davis 95616, USA
    J Mol Biol 252:157-62. 1995
    ..Furthermore, SpKRP85-SpKRP95 complexes can be immunoprecipitated from a cell-free translation system, providing direct evidence that SpKRP85 and SpKRP95 are capable of heterodimerization...
  59. ncbi Multiple controls of community structure and dynamics in a sublittoral marine environment
    Bernat Hereu
    Departament d Ecologia, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain
    Ecology 89:3423-35. 2008
    ..We conducted an experiment by manipulating densities of the major consumers of benthic algae (fishes and sea urchins) in approximately 100-m2 enclosures in a marine reserve, and monitored algal assemblages over two and a half ..
  60. pmc A spatially dynamic cohort of regulatory genes in the endomesodermal gene network of the sea urchin embryo
    Joel Smith
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Dev Biol 313:863-75. 2008
    ....
  61. ncbi The S. purpuratus genome: a comparative perspective
    Stefan C Materna
    Division of Biology, m c 139 74, California Institute of Technology, 1200 East California Blvd, Pasadena, CA 91125, USA
    Dev Biol 300:485-95. 2006
    ..models are not as abundant in the other genomes and thus constitute expansions that are specific at least to sea urchins if not to all echinoderms...
  62. ncbi A micromere induction signal is activated by beta-catenin and acts through notch to initiate specification of secondary mesenchyme cells in the sea urchin embryo
    D R McClay
    Department of Biology, DCMB Group, Box 91000, Duke University, Durham, NC 27708, USA
    Development 127:5113-22. 2000
    ..As Notch is maternally expressed in macromeres, additional components must be downstream of nuclear beta-catenin in macromeres for these cells to receive and transduce the micromere induction signal...
  63. pmc Speract induces calcium oscillations in the sperm tail
    Chris D Wood
    School of Cell and Molecular Biosciences, University of Newcastle upon Tyne, NE2 4HH, UK
    J Cell Biol 161:89-101. 2003
    ..Sperm are highly polarized cells. Our results indicate that a clear understanding of the link between [Ca2+]i and sperm motility will only be gained by analysis of [Ca2+]i signals at the level of the single sperm...
  64. ncbi Speract. Purification and characterization of a peptide associated with eggs that activates spermatozoa
    J R Hansbrough
    J Biol Chem 256:1447-52. 1981
    ..Gametes from 5000 female sea urchins were required for the isolation of approximately 9 mg of the peptide...
  65. ncbi Reproductive character displacement and the genetics of gamete recognition in tropical sea urchins
    Laura B Geyer
    Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Evolution 57:1049-60. 2003
    ..In sea urchins, the gamete recognition protein bindin evolves under positive selection when species are broadly sympatric, ..
  66. ncbi Sea urchin spine calcite forms via a transient amorphous calcium carbonate phase
    Yael Politi
    Department of Structural Biology, 76100 Rehovot, Israel
    Science 306:1161-4. 2004
    ..Deposition of transient amorphous phases as a strategy for producing single crystals with complex morphology may have interesting implications for the development of sophisticated materials...
  67. ncbi Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening
    Cristina Calestani
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Development 130:4587-96. 2003
    ..The results suggest that S. purpuratus pks, fmo and sult could belong to a differentiation gene battery of pigment cells...
  68. ncbi Sea urchin bindin divergence predicts gamete compatibility
    Kirk S Zigler
    Smithsonian Tropical Research Institute, Balboa, Panama
    Evolution 59:2399-404. 2005
    ..In this study, we surveyed data from the literature from five genera of sea urchins for which there was information on gamete compatibility, divergence of the sperm-egg recognition protein bindin,..
  69. ncbi Tuning sperm chemotaxis
    Adan Guerrero
    Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Av Universidad, 2001 Col Chamilpa, Cuernavaca, Morelos, Mexico
    Biochem Soc Trans 38:1270-4. 2010
    ..The article considers this new evidence and summarizes the known underlying cellular mechanisms and behavioural strategies that sperm use to locate and fertilize the oocyte...
  70. ncbi Range expansion of a habitat-modifying species leads to loss of taxonomic diversity: a new and impoverished reef state
    S D Ling
    School of Zoology, University of Tasmania, Private Bag 5, Hobart, 7001, Australia
    Oecologia 156:883-94. 2008
    ..Such a disproportionate effect by a single range-expanding species demonstrates that climate change may lead to unexpectedly large impacts on marine biodiversity as key habitat-modifying species undergo range modification...
  71. ncbi The immune gene repertoire encoded in the purple sea urchin genome
    Taku Hibino
    Sunnybrook Research Institute and Department of Medical Biophysics, University of Toronto, 2075 Bayview Ave, Room S 126B, Toronto, Ontario, Canada M4N 3M5
    Dev Biol 300:349-65. 2006
    ..b>Sea urchins are long-lived, complex organisms and these findings reveal an innate immune system of unprecedented complexity...
  72. pmc High accuracy, high-resolution prevalence measurement for the majority of locally expressed regulatory genes in early sea urchin development
    Stefan C Materna
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Gene Expr Patterns 10:177-84. 2010
    ..The data are available via an interactive website for quick plotting of selected time courses...
  73. ncbi The signal flow and motor response controling chemotaxis of sea urchin sperm
    U Benjamin Kaupp
    Institut für Biologische Informationsverarbeitung, Forschungszentrum Julich, 52425 Julich, Germany
    Nat Cell Biol 5:109-17. 2003
    ..Both resact and cyclic nucleotides cause a turn or brief tumbling in the swimming path of sperm. We conclude that a cGMP-mediated increase in the Ca2+ concentration induces the primary motor response of sperm to the chemoattractant...
  74. ncbi Spatial expression of Hox cluster genes in the ontogeny of a sea urchin
    C Arenas-Mena
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Development 127:4631-43. 2000
    ..The spatial expression patterns of the Hox genes illuminate the evolutionary process by which the pentameral echinoderm body plan emerged from a bilateral ancestor...
  75. ncbi cis-regulatory processing of Notch signaling input to the sea urchin glial cells missing gene during mesoderm specification
    Andrew Ransick
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Dev Biol 297:587-602. 2006
    ..While these Su(H) target sites provide the cis-regulatory architecture with the core of an N signaling transcriptional response switch, both the on and off outputs from this module require additional inputs...
  76. ncbi Diacylglycerol induces fusion of nuclear envelope membrane precursor vesicles
    Teresa Barona
    Biology Department, Amherst College, Amherst, Massachusetts 01002, USA
    J Biol Chem 280:41171-7. 2005
    ....
  77. ncbi Molecular phylogenies and divergence times of sea urchin species of Strongylocentrotidae, Echinoida
    Youn Ho Lee
    Polar Sciences Laboratory, Korea Ocean Research and Development Institute, Ansan, South Korea
    Mol Biol Evol 20:1211-21. 2003
    b>Sea urchins of the family Strongylocentrotidae have been important model systems in many fields of basic biology, yet knowledge of their evolutionary identities such as the phylogenetic relationships and divergence times remains limited...
  78. ncbi A glimpse into the molecular entrails of endoderm formation
    Didier Y R Stainier
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics, and Human Genetics, University of California, San Francisco, San Francisco, California 94143 0448, USA
    Genes Dev 16:893-907. 2002
  79. ncbi Cell-permeant NAADP: a novel chemical tool enabling the study of Ca2+ signalling in intact cells
    Raman Parkesh
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, United Kingdom
    Cell Calcium 43:531-8. 2008
    ..NAADP-AM is a powerful chemical tool that will be of enormous biological utility in a wide range of systems and will greatly facilitate research into the role of NAADP in health and disease...
  80. ncbi Sequential signaling crosstalk regulates endomesoderm segregation in sea urchin embryos
    Aditya J Sethi
    National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA
    Science 335:590-3. 2012
    ..Thus, we report that endomesoderm segregation is a progressive process, requiring a succession of regulatory interactions between cWnt and Notch signaling...
  81. ncbi A genetic regulatory network for Xenopus mesendoderm formation
    Matthew Loose
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, UK
    Dev Biol 271:467-78. 2004
    ..Wider comparisons of such networks will inform our understanding of developmental evolution...
  82. pmc New insights into mutable collagenous tissue: correlations between the microstructure and mechanical state of a sea-urchin ligament
    Ana R Ribeiro
    Instituto de Engenharia Biomedica, Biomaterials Division, NewTherapies Group, Universidade do Porto, Porto, Portugal
    PLoS ONE 6:e24822. 2011
    ..Further evidence that the juxtaligamental cells are the effectors of these changes in mechanical properties was provided by a correlation between their cytology and the tensile state of the CDLs...
  83. pmc Voltage-controlled gating at the intracellular entrance to a hyperpolarization-activated cation channel
    Brad S Rothberg
    Department of Neurobiology, Harvard Medical School, 220 Longwood Avenue, Boston, MA 02115, USA
    J Gen Physiol 119:83-91. 2002
    ..Together, these results are consistent with a voltage-controlled structure at the intracellular side of the spHCN channel that can gate the flow of cations through the pore...
  84. ncbi Sea urchin eggs in the acid reign
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, United Kingdom
    Cell Calcium 50:147-56. 2011
    ..This review attempts to summarize what we currently know about egg acidic vesicles in the context of Ca(2+) signalling. The dynamics of Ca(2+) storage, Ca(2+) mobilization, proton fluxes and two-pore channels will be discussed...
  85. pmc The endoderm gene regulatory network in sea urchin embryos up to mid-blastula stage
    Isabelle S Peter
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Dev Biol 340:188-99. 2010
    ..Here we integrate the architecture of regulatory interactions with the spatial restriction of regulatory gene expression to model the logic control of endoderm development...
  86. ncbi Frizzled5/8 is required in secondary mesenchyme cells to initiate archenteron invagination during sea urchin development
    Jenifer Croce
    Unite de Biologie du Developpement, UMR 7009, CNRS, Universite Pierre et Marie Curie, Observatoire Oceanologique, Villefranche sur Mer, France
    Development 133:547-57. 2006
    ..Taken together, the results suggest that Fz5/8 plays a crucial role specifically in SMCs to control primary invagination during sea urchin gastrulation...
  87. ncbi Measurement of the intracellular pH threshold for sperm aster formation in sea urchin eggs
    M S Hamaguchi
    Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152-8551, Japan
    Dev Growth Differ 43:447-58. 2001
    ..0 or more. Consequently, whether a Cai increase on fertilization occurs or not, the threshold pHi value for sperm aster formation is constant in sea urchin eggs...
  88. pmc Transcriptional regulatory cascades in development: initial rates, not steady state, determine network kinetics
    Hamid Bolouri
    Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103 8904, USA
    Proc Natl Acad Sci U S A 100:9371-6. 2003
    ..The kinetics of such developmental systems thus depend mainly on the initial output rates of genes activated in response to the advent of new transcription factors...
  89. ncbi Isolation and culture of micromeres and primary mesenchyme cells
    Fred H Wilt
    Department of Molecular Cell Biology, University of California, Berkeley, California 94720, USA
    Methods Cell Biol 74:273-85. 2004
  90. ncbi Functional characterization of the sea urchin sns chromatin insulator in erythroid cells
    Santina Acuto
    Unità di Ricerca P Cutino, Ematologia II, A O V Cervello, via Trabucco n 180 90146, Palermo, Italy
    Blood Cells Mol Dis 35:339-44. 2005
    ....
  91. ncbi Adaptive evolution of sperm bindin tracks egg incompatibility in neotropical sea urchins of the genus Echinometra
    Michael A McCartney
    Smithsonian Tropical Research Institute, Balboa, Republic of Panama
    Mol Biol Evol 21:732-45. 2004
    ..is a gamete recognition protein known to control species-specific sperm-egg adhesion and membrane fusion in sea urchins. Previous analyses have shown that diversifying selection on bindin amino acid sequence is found when ..
  92. ncbi Quantitative analysis of gametic incompatibility between closely related species of neotropical sea urchins
    Michael A McCartney
    Smithsonian Tropical Research Institute, Apartado 2072, Balboa, Republic of Panama
    Biol Bull 202:166-81. 2002
    ..Compensatory sexual selection on sperm in this species could follow, and promote divergence of proteins mediating sperm-egg recognition...
  93. ncbi Lack of character displacement in the male recognition molecule, bindin, in Altantic sea urchins of the genus Echinometra
    Laura B Geyer
    Naos Marine Laboratories, Smithsonian Tropical Research Institute, Panama, Republica de Panama
    Mol Biol Evol 26:2135-46. 2009
    ..Processes acting within species, such as sexual selection, sperm competition, or sexual conflict, are more likely to be involved in the evolution of this molecule...
  94. ncbi Assessment of the use potential of edible sea urchins (Paracentrotus lividus) processing waste within the agricultural system: influence on soil chemical and biological properties and bean (Phaseolus vulgaris) and wheat (Triticum vulgare) growth in an ame
    Giovanni Garau
    Sezione di Scienze e Tecnologie Ambientali e Alimentari, Dipartimento di Agraria, University of Sassari, Viale Italia 39, 07100 Sassari, Italy
    J Environ Manage 109:12-8. 2012
    ..lividus processing waste, which foresees economic value in the sea urchin by-product through its re-use within the agricultural production system...
  95. pmc SpCoel1: a sea urchin profilin gene expressed specifically in coelomocytes in response to injury
    L C Smith
    Division of Biology, California Institute of Technology, Pasadena 91125
    Mol Biol Cell 3:403-14. 1992
    ....
  96. ncbi Transcriptomic responses to ocean acidification in larval sea urchins from a naturally variable pH environment
    Tyler G Evans
    Department of Ecology, Evolution and Marine Biology, University of California Santa Barbara, Santa Barbara, CA 93106 9620, USA
    Mol Ecol 22:1609-25. 2013
    ..Next, we cultured the progeny of adult purple sea urchins (Strongylocentrotus purpuratus) collected from this region in CO2 -acidified seawater representing present day ..
  97. ncbi Involvement of Tcf/Lef in establishing cell types along the animal-vegetal axis of sea urchins
    L Huang
    Department of Biochemistry and Molecular Biology, M D Anderson Cancer Center, University of Texas, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Dev Genes Evol 210:73-81. 2000
    ..To examine the role of Tcf/Lef in sea urchins we cloned a Strongylocentrotus purpuratus Tcf/Lef homolog...
  98. ncbi Detecting expression patterns of Wnt pathway components in sea urchin embryos
    Joanna M Bince
    Department of Zoology, The University of Hawaii at Manoa, Honolulu, HI, USA
    Methods Mol Biol 469:201-11. 2008
    ..Here, we provide detailed protocols for determining the expression and localization of mRNA and proteins in early sea urchin embryos, which can be used in studies examining the regulation of Wnt signaling along the A-V axis...
  99. ncbi Characterization of matrix metalloprotease activities induced in the sea urchin extraembryonic matrix, the hyaline layer
    C Sharpe
    Department of Biochemistry, Memorial University of Newfoundland, St John's, Canada
    Biochem Cell Biol 79:461-8. 2001
    ..These conclusions are discussed in the context of the high calcium and magnesium concentrations present in the sea water environment of the sea urchin embryo...
  100. ncbi How to grow a gut: ontogeny of the endoderm in the sea urchin embryo
    G M Wessel
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Bioessays 21:459-71. 1999
    ..Recent progress at the molecular level should soon allow us to explain the seminal experimental observations made in this embryo over a hundred years ago...
  101. ncbi Cyclic ADP-ribose
    A H Guse
    University of Hamburg, Institute for Medical Biochemistry and Molecular Biology, Division of Cellular Signal Transduction, Germany
    J Mol Med (Berl) 78:26-35. 2000
    ....

Research Grants63

  1. Purchase of a nanoLC-QTRAP Mass Spectrometer for Targeted Proteomics Studies
    Sonja Hess; Fiscal Year: 2013
    ..In addition, the Davidson laboratory will study a defined set of transcription factors in sea urchins that will delineate the spatial specifications of gene expression in sea urchin embryos...
  2. Characterization of Aging in Sea Urchin Species with Different Life Spans
    Andrea Bodnar; Fiscal Year: 2012
    ..As an exception, sea urchins present a unique and tractable model for the study of aging and negligible senescence...
  3. Gene regulatory network evolution and the origin of biological novelties
    MARK MARTINDALE; Fiscal Year: 2013
    ..the specification of endomesodermal cell fates have been constructed in a handful of model systems, include sea urchins, vertebrates, and nematodes...
  4. Structure and Function in Notch Signaling
    Stephen C Blacklow; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Notch receptors anchor a fundamental signaling pathway conserved from sea urchins to humans...
  5. Gene regulatory study of pigment cell development in sea urchins
    Cristina Calestani; Fiscal Year: 2010
    ....
  6. Control of Multidrug Transport Activity in Embryos
    Amro M Hamdoun; Fiscal Year: 2011
    ..cell surface changes in early embryo development and changes in the efflux transporter activity focusing on sea urchins as an easily assayed and manipulated model organism...
  7. EMBRYOLOGY COURSE
    ALEJANDRO SANCHEZ ALVARADO; Fiscal Year: 2013
    ..elegans, Drosophila, planarians, ascidians, sea urchins, zebrafish, amphibians, chick and mouse...
  8. Regulators of Dishevelled Function in the Wnt Signaling Pathway
    Athula H Wikramanayake; Fiscal Year: 2013
    ..In sea urchins, Dvl puncta accumulate in a specialized domain in the egg cortex at the vegetal pole, and these structures ..
  9. Control of Early Embryonic Cell Polarity in a Model Deuterostome
    David Burgess; Fiscal Year: 2010
    ..The animal kingdom is divided into two major groups based on how their embryos develop. Echinoderms, including sea urchins, develop like lower and higher chordates whereas other genetic model organisms heavily studied for their ..
  10. SYMPOSIUM ON MODEL INVERTEBRATE SYSTEMS FOR THE STUDY OF STEM CELLS IN DEVELOPMEN
    Jeffrey L Ram; Fiscal Year: 2013
    ..their topics range from regeneration in Ciona (a basal chordate), to analysis of aging mechanisms in "ageless" sea urchins and their short-lived cousins, to Hydra, "the everlasting embryo "...
  11. Mechanisms of Adhesive Interactions
    STEVEN OPPENHEIMER; Fiscal Year: 2009
    ..molecules in live embryos blocks the cellular interaction; (7) if the findings are similar in two species of sea urchins. This study is innovative and novel because the system is totally accessible to probes in living embryos and ..
  12. Structure and function of ABC transporters to understand persistence of global ma
    Amro M Hamdoun; Fiscal Year: 2013
    ..conservation of the major sub-family types (ABCB, ABCC and ABCG) of xenobiotic eliminating transporters between sea urchins and man...
  13. Beyond GFP and aequorin: Ocean-wide study of fluorescent and luminous proteins
    Mikhail Matz; Fiscal Year: 2009
    ..assembled that includes spectacular taxonomic diversity of animals, ranging from radiolarians and jellyfish to sea urchins and fish...
  14. A C. elegans localization-of-expression mapping project
    Marc Vidal; Fiscal Year: 2005
    ..the best illustrations of this statement is the discovery of cyclins and cyclin-dependent kinases in yeasts and sea urchins. Other examples of the role of C...
  15. A C. elegans localization-of-expression mapping project
    Marc Vidal; Fiscal Year: 2002
    ..the best illustrations of this statement is the discovery of cyclins and cyclin-dependent kinases in yeasts and sea urchins. Other examples of the role of C...
  16. Delta and Mesoderm Induction in Eucidaris Embryos
    Hyla Sweet; Fiscal Year: 2004
    ..The embryos of derived sea urchins have four micromeres at the vegetal pole that have powerful organizing abilities...
  17. A GENE FAMILY EXPRESSED IN SEA URCHIN EMBRYONIC ECTODERM
    WILLIAM KLEIN; Fiscal Year: 2005
    ..be used as a model system to address the key issues of cell type-specific gene activation during development Sea urchins offer unique opportunities for developmental analysis because of their close evolutionary and developmental ..
  18. Developmental Biology of Sea Urchins-XV
    ROBERT ANGERER; Fiscal Year: 2003
    ..Invitees will include all those interested in cell, development, and evolutionary mechanism in sea urchins, tunicates, hemichordates and cephalochordates...
  19. Comparative analysis of genes required for skeleton formation in sea urchins
    Laura Romano; Fiscal Year: 2009
    ..the extent to which there are differences in the cis-regulatory region of SM50 among closely related species of sea urchins, and to evaluate their functional significance...
  20. CRYSTALLOGRAPHIC & SOLUTION STUDIES OF HISTONES
    EVANGELOS MOUDRIANAKIS; Fiscal Year: 1990
    ..from highly differentiated systems (chicken erythrocyte, calf thymus, sea urchin sperm), embryonic systems (sea urchins), and mammalian cells in culture (HL60, HEp-2 & NIH 3T3,+ butyrate, etc.)...
  21. Evolution of sperm morphology genes in sea urchins
    MOLLIE MANIER; Fiscal Year: 2007
    ..This project will be implemented using sea urchins, aided by a vast understanding of their reproductive and developmental biology as well as an ongoing genome ..
  22. Misregulation of apoptosis in cloned pig embryos
    Heide Schatten; Fiscal Year: 2007
    ..Pilot data from this R03 small grants program research will be used to apply for funding through the R01 mechanism. [unreadable] [unreadable] [unreadable]..
  23. REGULATION OF BETA-CATENIN NUCLEARIZATION
    CHARLES ETTENSOHN; Fiscal Year: 2006
    ..In addition, using dominant negative constructs and morpholinos, we will examine the developmental function of two regulators of GSK3 activity, disheveled and Akt/PKB. ..
  24. MECHANISM AND REGULATION OF ACTIN-BASED RETROGRADE FLOW
    JOHN HENSON; Fiscal Year: 2005
    ..4. To study the inducible transformation of coelomocytes from a lamellipodial to a filopodial form. ..
  25. CONFOCAL MICROSCOPE
    Gary Wessel; Fiscal Year: 2003
    ..The requested instrument would significantly enhance the research productivity of the broad, interdisciplinary community of researchers described here, and greatly improve the biological image capabilities in the region. ..
  26. Mitochondria distribution in cloned pig embryos
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 small grants program research will be used to apply for funding through the RO1 mechanism. ..
  27. Cytoskeletal organization in apicomplexan parasites
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 research will be used to apply for funding through the RO1 mechanism. ..
  28. Regulatory Genomics: BAC-GFP Library of Control Genes
    Eric Davidson; Fiscal Year: 2008
    ..Numerous letters from outside investigators attesting interest in this facility are included as an Appendix to this application. ..
  29. Sea Urchin Developmental Biology Conference XIV
    DAVID MC CLAY; Fiscal Year: 2002
    ..meeting has special importance because of three genome projects being conducted (two on tunicates and one on sea urchins)...
  30. Mechanism of cytochrome P450 uncoupling and inactivation
    JARED GOLDSTONE; Fiscal Year: 2005
    ..Recently identified AhR-agonistic persistent pollutants, such as polybrominated diphenyl ethers will also be investigated. ..
  31. GERM LAYER SPECIFICATION
    DAVID MC CLAY; Fiscal Year: 2007
    ..This network eventually will enable us to explore how positional cues are produced and displayed for later morphogenetic patterning. ..
  32. NEURAL TUBE DEFECTS
    DAVID MC CLAY; Fiscal Year: 2005
    ..abstract_text> ..
  33. Redox-sensitive developmental pathways and gene regulatory networks
    JAMES COFFMAN; Fiscal Year: 2009
    ..Finally, the Comparative Toxicogenomics Database (CTD) at MDIBL will be used to determine the relevance of the pathways discovered in sea urchins to human health, and to generate hypotheses that might explain specific human diseases.
  34. Computational Modeling of Developmental Processes
    LARRY TABER; Fiscal Year: 2009
    ..This software will open up new avenues for studying the mechanics of development in the cardiovascular, nervous, and other systems composed of soft tissue. ..
  35. Control of Cell Proliferation by Runx Proteins
    JAMES COFFMAN; Fiscal Year: 2009
    ..purpuratus genome sequence, which will greatly facilitate both the cis-regulatory analysis of genes and the identification of purified proteins by mass spectrometry. ..
  36. BIOMECHANICS OF LOOPING IN THE EMBRYONIC HEART
    LARRY TABER; Fiscal Year: 2003
    ..Defining the biomechanical forces involved in looping would provide insight into this morphogenetic process and thereby help researchers searching for the link between gene expression and looping morphology. ..
  37. Computational and Experimental Study of Early Cardiac Morphogenesis
    LARRY TABER; Fiscal Year: 2009
    ..In addition, the computational model created during the course of our research may someday lead to advanced models of the developing human heart, which is not amenable to direct experimental study. ..
  38. Spiralian developmental system for the study of multipotency
    Cesar Arenas Mena; Fiscal Year: 2008
    ..The ultimate goal is to manipulate these genes and reprogram in vivo differentiated cells into multipotent stem cells. [unreadable] [unreadable] [unreadable]..
  39. ROLE OF NITRIC OXIDE IN FERTILIZATION
    David Epel; Fiscal Year: 2002
    ..The possible role of NO in regulating the egg's ion channels (as part of polyspermy block) and sperm physiology (such as motility and the acrosome reaction) will also be investigated. ..
  40. Signaling activities of amelogenin gene splice products.
    Arthur Veis; Fiscal Year: 2006
    ..In this revision, more experimental details have been provided. ..
  41. MOLECULAR PHYSIOLOGY OF POTASSIUM CHANNELS
    Gary Yellen; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  42. STRUCTURE & ASSEMBLY OF COLLAGEN MOLECULES & FIBRILS
    Arthur Veis; Fiscal Year: 2004
    ..The final study (4) will examine N-telopeptide structures as related to molecular assembly into fibrils and the cross-link patterns that modulate packing. These studies will help in understanding dysfunction in humans. ..
  43. STRUCTURE-FUNCTION OF ADP-RIBOSYL CYCLASE AND HOMOLOGS
    HONCHEUNG LEE; Fiscal Year: 2003
    ..3. To determine the structure of the active site of the cyclase by X-ray crystallography. 4. To develop an inducible expression system of the cyclase for use in physiological studies. ..
  44. FUNCTION OF SEX SPECIFIC EXONS IN DNMT1 GENE
    Timothy Bestor; Fiscal Year: 2002
    ....
  45. NOVEL DNA METHYLTRANSFERASES IN DEVELOPMENT AND DISEASE
    Timothy Bestor; Fiscal Year: 2002
    ..abstract_text> ..
  46. SMALL MOLECULE REGULATION OF BREAST CANCER EXPRESSION
    JOEL GOTTESFELD; Fiscal Year: 2002
    ..Toxicity of the polyamides will be assessed in cell-based assays and non-specific effects of the polyamides on genome-wide gene expression will be monitored using high density DNA array/hybridization methodology. ..
  47. CCD Camera for Intermediate Voltage Electron Microscopes
    Kenneth H Downing; Fiscal Year: 2010
    ..These adaptations will produce substantial benefits in work ranging from high-resolution structural studies of proteins and viruses to the three dimensional study of cell ultrastructure. ..
  48. RNA PROCESSING AND RIBONUCLEOPROTEIN
    Thoru Pederson; Fiscal Year: 2001
    ..The same studies should provide information on sequences required for nuclear export. These two inter-related projects test the hypothesis that the nucleolus is a more pluri-functional organelle than previously thought. ..
  49. Methylation Landscape of the Human Genome
    Timothy Bestor; Fiscal Year: 2003
    ..The impending completion of the sequence of the human genome presents a unique opportunity to gain understanding of the shape of genomic methylation patterns and their role in human development and disease. ..
  50. Phosphorylation dependent recognition of a histone mRNA hairpin by SLBP
    William Marzluff; Fiscal Year: 2009
    ..abstract_text> ..
  51. Genetic Control of Tubulin Function in Development
    ELIZABETH RAFF; Fiscal Year: 2005
    ..The primary aim is to determine how transient expression of beta3-tubulin during development modulates the microtubule cytoskeleton in the differentiated cell. ..
  52. Dynamic Interactions of IP3 Receptor Ligands
    BARBARA EHRLICH; Fiscal Year: 2004
    ....
  53. CONTROLLING GENE EXPRESSION WITH PEPTIDE NUCLEIC ACIDS
    David Corey; Fiscal Year: 2007
    ..The work proposed here will rigorously test the value of antisense PNAs and our data will shape decisions regarding the use of PNAs for laboratory studies and clinical development. ..
  54. Histone Deacetylase Inhibitors as Therapeutics for Friedreich's ataxia
    JOEL GOTTESFELD; Fiscal Year: 2007
    ..We are developing small molecules to reverse this effect, with the hope that these molecules may be therapeutic for FRDA. [unreadable] [unreadable] [unreadable]..
  55. PROFESSORS OF THE FUTURE: POST DOC CAREER DEVELOPMENT
    JERRY HEDRICK; Fiscal Year: 2006
    ..abstract_text> ..
  56. 3-D Image Restoration for Polarized Light Microscopy
    Rudolf Oldenbourg; Fiscal Year: 2003
    ..The image restoration procedures will greatly enhance the utility of the P01-Scope for biological and medical applications to non-invasively analyze the architectural dynamics of living cells. ..
  57. TUBULIN STRUCTURE BY ELECTRON CRYSTALLOGRAPHY
    KENNETH DOWNING; Fiscal Year: 2003
    ..abstract_text> ..
  58. Recognition of Chromosomal DNA by Double-Stranded RNA
    David R Corey; Fiscal Year: 2010
    ..Specifically, our work will expand the options available for designing RNA drugs to reduce gene expression and fills an unmet need by providing a new strategy for designing RNAs to increase gene expression. ..
  59. INHIBITION OF HIV GENE TRANSCRIPTION BY SMALL MOLECULES
    JOEL GOTTESFELD; Fiscal Year: 2004
    ..Given our success in inhibition of virus replication in cell culture, we propose collaborative preclinical studies with the Mosier laboratory to determine the effectiveness of polyamides in the human PBL-SCID mouse model. ..