drosophila melanogaster

Summary

Summary: A species of fruit fly much used in genetics because of the large size of its chromosomes.

Top Publications

  1. pmc Identification of functional elements and regulatory circuits by Drosophila modENCODE
    Sushmita Roy
    Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology MIT, Cambridge, MA 02139, USA
    Science 330:1787-97. 2010
  2. pmc A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3
    Pablo Cingolani
    Institute of Environmental Health Sciences, Wayne State University, Detroit, MI, USA
    Fly (Austin) 6:80-92. 2012
  3. pmc The Drosophila melanogaster Genetic Reference Panel
    Trudy F C Mackay
    Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA
    Nature 482:173-8. 2012
  4. pmc The developmental transcriptome of Drosophila melanogaster
    Brenton R Graveley
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 6403, USA
    Nature 471:473-9. 2011
  5. pmc FlyBase 101--the basics of navigating FlyBase
    Peter McQuilton
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    Nucleic Acids Res 40:D706-14. 2012
  6. pmc An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases
    Johannes Bischof
    Frontiers in Genetics, National Center of Competence in Research, Institute of Molecular Biology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Proc Natl Acad Sci U S A 104:3312-7. 2007
  7. ncbi A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
    Georg Dietzl
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, A 1030 Vienna, Austria
    Nature 448:151-6. 2007
  8. pmc MicroRNA targets in Drosophila
    Anton J Enright
    Computational Biology Center, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Genome Biol 5:R1. 2003
  9. ncbi The host defense of Drosophila melanogaster
    Bruno Lemaitre
    Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
    Annu Rev Immunol 25:697-743. 2007
  10. ncbi Using FlyAtlas to identify better Drosophila melanogaster models of human disease
    Venkateswara R Chintapalli
    Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, UK
    Nat Genet 39:715-20. 2007

Detail Information

Publications347 found, 100 shown here

  1. pmc Identification of functional elements and regulatory circuits by Drosophila modENCODE
    Sushmita Roy
    Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology MIT, Cambridge, MA 02139, USA
    Science 330:1787-97. 2010
    ..Our results provide a foundation for directed experimental and computational studies in Drosophila and related species and also a model for systematic data integration toward comprehensive genomic and functional annotation...
  2. pmc A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3
    Pablo Cingolani
    Institute of Environmental Health Sciences, Wayne State University, Detroit, MI, USA
    Fly (Austin) 6:80-92. 2012
    ..SNPs in ~117 Mb unique sequences, representing a substitution rate of ~1/305 nucleotides, between the Drosophila melanogaster w(1118); iso-2; iso-3 strain and the reference y(1); cn(1) bw(1) sp(1) strain...
  3. pmc The Drosophila melanogaster Genetic Reference Panel
    Trudy F C Mackay
    Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA
    Nature 482:173-8. 2012
    ..Here, we describe the Drosophila melanogaster Genetic Reference Panel (DGRP), a community resource for analysis of population genomics and quantitative ..
  4. pmc The developmental transcriptome of Drosophila melanogaster
    Brenton R Graveley
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 6403, USA
    Nature 471:473-9. 2011
    b>Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites...
  5. pmc FlyBase 101--the basics of navigating FlyBase
    Peter McQuilton
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    Nucleic Acids Res 40:D706-14. 2012
    ..org) is the leading database and web portal for genetic and genomic information on the fruit fly Drosophila melanogaster and related fly species...
  6. pmc An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases
    Johannes Bischof
    Frontiers in Genetics, National Center of Competence in Research, Institute of Molecular Biology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Proc Natl Acad Sci U S A 104:3312-7. 2007
    Germ-line transformation via transposable elements is a powerful tool to study gene function in Drosophila melanogaster. However, some inherent characteristics of transposon-mediated transgenesis limit its use for transgene analysis...
  7. ncbi A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
    Georg Dietzl
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3 5, A 1030 Vienna, Austria
    Nature 448:151-6. 2007
    ..Here we report the generation and validation of a genome-wide library of Drosophila melanogaster RNAi transgenes, enabling the conditional inactivation of gene function in specific tissues of the intact ..
  8. pmc MicroRNA targets in Drosophila
    Anton J Enright
    Computational Biology Center, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Genome Biol 5:R1. 2003
    ..and characterization of the first few target genes regulated by miRNAs in Caenorhabditis elegans and Drosophila melanogaster have set the stage for elucidation of a novel network of regulatory control...
  9. ncbi The host defense of Drosophila melanogaster
    Bruno Lemaitre
    Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
    Annu Rev Immunol 25:697-743. 2007
    To combat infection, the fruit fly Drosophila melanogaster relies on multiple innate defense reactions, many of which are shared with higher organisms...
  10. ncbi Using FlyAtlas to identify better Drosophila melanogaster models of human disease
    Venkateswara R Chintapalli
    Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, UK
    Nat Genet 39:715-20. 2007
    ..a new online resource, provides the most comprehensive view yet of expression in multiple tissues of Drosophila melanogaster. Meta-analysis of the data shows that a significant fraction of the genome is expressed with great tissue ..
  11. pmc Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators
    Lin Tian
    Howard Hughes Medical Institute, Ashburn, Virginia, USA
    Nat Methods 6:875-81. 2009
    ..In Caenorhabditis elegans chemosensory neurons and the Drosophila melanogaster antennal lobe, sensory stimulation-evoked fluorescence responses were significantly enhanced with GCaMP3 (..
  12. ncbi The evolutionary fate and consequences of duplicate genes
    M Lynch
    Department of Biology, University of Oregon, Eugene, OR 97403, USA
    Science 290:1151-5. 2000
    ..Although duplicate genes may only rarely evolve new functions, the stochastic silencing of such genes may play a significant role in the passive origin of new species...
  13. pmc Genome sequence of Aedes aegypti, a major arbovirus vector
    Vishvanath Nene
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 316:1718-23. 2007
    ..gambiae and Drosophila melanogaster. Nonetheless, chromosomal synteny is generally maintained among all three insects, although conservation ..
  14. pmc A GAL4-driver line resource for Drosophila neurobiology
    Arnim Jenett
    Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA
    Cell Rep 2:991-1001. 2012
    We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer...
  15. pmc Systematic protein location mapping reveals five principal chromatin types in Drosophila cells
    Guillaume J Filion
    Division of Gene Regulation, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Cell 143:212-24. 2010
    ..Finally, we provide evidence that the different chromatin types help to target DNA-binding factors to specific genomic regions. These results provide a global view of chromatin diversity and domain organization in a metazoan cell...
  16. ncbi Inhibition of mTOR induces autophagy and reduces toxicity of polyglutamine expansions in fly and mouse models of Huntington disease
    Brinda Ravikumar
    Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2XY, UK
    Nat Genet 36:585-95. 2004
    ..Our data provide proof-of-principle for the potential of inducing autophagy to treat Huntington disease...
  17. pmc Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolution
    D Allan Drummond
    FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 134:341-52. 2008
    ....
  18. ncbi bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila
    Julius Brennecke
    European Molecular Biology Laboratory, Meyerhofstr 1, 69117 Heidelberg, Germany
    Cell 113:25-36. 2003
    ..bantam microRNA simultaneously stimulates cell proliferation and prevents apoptosis. We identify the pro-apoptotic gene hid as a target for regulation by bantam miRNA, providing an explanation for bantam's anti-apoptotic activity...
  19. pmc Comprehensive analysis of the chromatin landscape in Drosophila melanogaster
    Peter V Kharchenko
    Center for Biomedical Informatics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 471:480-5. 2011
    ..Here we present a genome-wide chromatin landscape for Drosophila melanogaster based on eighteen histone modifications, summarized by nine prevalent combinatorial patterns...
  20. ncbi Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila
    Julius Brennecke
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, NY 11724, USA
    Cell 128:1089-103. 2007
    ..Thus, sense piRNAs, formed following cleavage of transposon mRNAs may enhance production of antisense piRNAs, complementary to active elements, by directing cleavage of transcripts from master control loci...
  21. pmc The hippo signaling pathway in development and cancer
    Duojia Pan
    Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 19:491-505. 2010
    ..These studies suggest that the core Hippo kinase cascade integrates multiple upstream inputs, enabling dynamic regulation of tissue homeostasis in animal development and physiology...
  22. ncbi A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    G Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Science 293:834-8. 2001
    ..We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce ..
  23. ncbi P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster
    Koen J T Venken
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 314:1747-51. 2006
    We describe a transgenesis platform for Drosophila melanogaster that integrates three recently developed technologies: a conditionally amplifiable bacterial artificial chromosome (BAC), recombineering, and bacteriophage PhiC31-mediated ..
  24. ncbi Transposable elements and the epigenetic regulation of the genome
    R Keith Slotkin
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Rev Genet 8:272-85. 2007
    ..In addition, new insights have been gained into how this silencing has been co-opted to serve essential functions in 'host' cells, highlighting the importance of TEs in the epigenetic regulation of the genome...
  25. ncbi Identification of novel genes coding for small expressed RNAs
    M Lagos-Quintana
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
    Science 294:853-8. 2001
    ..This suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated...
  26. pmc A cis-regulatory map of the Drosophila genome
    Nicolas Negre
    Institute for Genomics and Systems Biology, Department of Human Genetics, The University of Chicago, 900 East 57th Street, Chicago, Illinois 60637, USA
    Nature 471:527-31. 2011
    ..Here we describe results from the modENCODE cis-regulatory annotation project. We produced a map of the Drosophila melanogaster regulatory genome on the basis of more than 300 chromatin immunoprecipitation data sets for eight ..
  27. pmc Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31
    Amy C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
    Genetics 166:1775-82. 2004
    ..Here we show that this site-specific integration system also functions efficiently in Drosophila melanogaster in cultured cells and in embryos...
  28. pmc The many landscapes of recombination in Drosophila melanogaster
    Josep M Comeron
    Department of Biology, University of Iowa, Iowa City, Iowa, USA
    PLoS Genet 8:e1002905. 2012
    ..We characterized the products of 5,860 female meioses in Drosophila melanogaster by genotyping a total of 139 million informative SNPs and mapped 106,964 recombination events at a ..
  29. pmc Genomic variation in natural populations of Drosophila melanogaster
    Charles H Langley
    Department of Evolution and Ecology, University of California, Davis, CA 95616, USA
    Genetics 192:533-98. 2012
    This report of independent genome sequences of two natural populations of Drosophila melanogaster (37 from North America and 6 from Africa) provides unique insight into forces shaping genomic polymorphism and divergence...
  30. ncbi Mutations affecting segment number and polarity in Drosophila
    C Nusslein-Volhard
    Nature 287:795-801. 1980
    In systematic searches for embryonic lethal mutants of Drosophila melanogaster we have identified 15 loci which when mutated alter the segmental pattern of the larva. These loci probably represent the majority of such genes in Drosophila...
  31. pmc Evidence for compensatory upregulation of expressed X-linked genes in mammals, Caenorhabditis elegans and Drosophila melanogaster
    Xinxian Deng
    Department of Pathology, University of Washington School of Medicine, Seattle, USA
    Nat Genet 43:1179-85. 2011
    ..elegans and Drosophila. Our analyses, which take into account the skewed gene content of the X chromosome, support the hypothesis of upregulation of expressed X-linked genes to balance expression of the genome...
  32. pmc Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo
    Richard Benton
    Laboratory of Neurogenetics and Behavior, The Rockefeller University, New York, New York, USA
    PLoS Biol 4:e20. 2006
    ....
  33. ncbi Adaptive protein evolution at the Adh locus in Drosophila
    J H McDonald
    Department of Ecology and Evolutionary Biology, Princeton University, New Jersey 08544
    Nature 351:652-4. 1991
    ..DNA sequence data on the Adh locus (encoding alcohol dehydrogenase, EC 1.1.1.1) in three species in the Drosophila melanogaster species subgroup do not fit this expectation; instead, there are more fixed replacement differences ..
  34. ncbi Drosophila pink1 is required for mitochondrial function and interacts genetically with parkin
    Ira E Clark
    Nature 441:1162-6. 2006
    ..The role of the pink1-parkin pathway in regulating mitochondrial function underscores the importance of mitochondrial dysfunction as a central mechanism of Parkinson's disease pathogenesis...
  35. ncbi Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channels
    Dieter Wicher
    Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans Knöll St 8, D 07745 Jena, Germany
    Nature 452:1007-11. 2008
    ..Thereby, they provide rapid and transient as well as sensitive and prolonged odorant signalling...
  36. ncbi Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome
    Annette L Parks
    Exelixis, 170 Harbor Way, South San Francisco, California 94083 0511, USA
    Nat Genet 36:288-92. 2004
    ..The latter methodology enables the generation of small custom deletions with predictable endpoints throughout the genome and should make their isolation a simple and routine task...
  37. ncbi Coding of odors by a receptor repertoire
    Elissa A Hallem
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 125:143-60. 2006
    ..The space provides a basis for predicting behavioral responses to odors...
  38. pmc A protein complex network of Drosophila melanogaster
    K G Guruharsha
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 147:690-703. 2011
    ..The DPiM represents, to our knowledge, the largest metazoan protein complex map and provides a valuable resource for analysis of protein complex evolution...
  39. ncbi HDAC6 rescues neurodegeneration and provides an essential link between autophagy and the UPS
    Udai Bhan Pandey
    Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nature 447:859-63. 2007
    ..Here we show that autophagy acts as a compensatory degradation system when the UPS is impaired in Drosophila melanogaster, and that histone deacetylase 6 (HDAC6), a microtubule-associated deacetylase that interacts with ..
  40. ncbi Role of histone H2A ubiquitination in Polycomb silencing
    Hengbin Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nature 431:873-8. 2004
    ..Thus, our studies identify the H2A ubiquitin ligase, and link H2A ubiquitination to Polycomb silencing...
  41. pmc Drosophila parkin requires PINK1 for mitochondrial translocation and ubiquitinates mitofusin
    Elena Ziviani
    Department of Biomedical Sciences, Medical Research Council Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield S10 2TN, United Kingdom
    Proc Natl Acad Sci U S A 107:5018-23. 2010
    ..We hypothesize that Mfn ubiquitination may provide a mechanism by which terminally damaged mitochondria are labeled and sequestered for degradation by autophagy...
  42. pmc Sequencing of pooled DNA samples (Pool-Seq) uncovers complex dynamics of transposable element insertions in Drosophila melanogaster
    Robert Kofler
    Institut für Populationsgenetik, Vetmeduni Vienna, Wien, Austria
    PLoS Genet 8:e1002487. 2012
    ..We apply this method to data from a natural Drosophila melanogaster population from Portugal...
  43. pmc A genome-scale shRNA resource for transgenic RNAi in Drosophila
    Jian Quan Ni
    Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts, USA
    Nat Methods 8:405-7. 2011
    Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system ..
  44. ncbi Asymmetry in the assembly of the RNAi enzyme complex
    Dianne S Schwarz
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, MA 01605, USA
    Cell 115:199-208. 2003
    ..Thus, the common step of RISC assembly is an unexpected source of asymmetry for both siRNA function and miRNA biogenesis...
  45. pmc BioGRID: a general repository for interaction datasets
    Chris Stark
    Ontario Cancer Institute, Princess Margaret Hospital, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9
    Nucleic Acids Res 34:D535-9. 2006
    ..0 includes >116 000 interactions from Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster and Homo sapiens...
  46. pmc Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAs
    Qingfa Wu
    Department of Plant Pathology and Microbiology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA
    Proc Natl Acad Sci U S A 107:1606-11. 2010
    ..We also report detection of virus-derived PIWI-interacting RNAs (piRNAs) in Drosophila melanogaster that have not been previously described in any other host species and demonstrate viral genome assembly ..
  47. pmc Mechanisms of life span extension by rapamycin in the fruit fly Drosophila melanogaster
    Ivana Bjedov
    Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, UK
    Cell Metab 11:35-46. 2010
    ..Rapamycin could increase life span of weak insulin/Igf signaling (IIS) pathway mutants and of flies with life span maximized by dietary restriction, indicating additional mechanisms...
  48. ncbi Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfaction
    Mattias C Larsson
    Laboratory of Neurogenetics and Behavior, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Neuron 43:703-14. 2004
    ..Consistent with this cellular defect, the Or83b mutation disrupts behavioral and electrophysiological responses to many odorants. Or83b therefore encodes an atypical odorant receptor that plays an essential general role in olfaction...
  49. pmc TRANSFAC and its module TRANSCompel: transcriptional gene regulation in eukaryotes
    V Matys
    BIOBASE GmbH, Halchtersche Strasse 33, D 38304 Wolfenbuttel, Germany
    Nucleic Acids Res 34:D108-10. 2006
    ..The here described public releases, TRANSFAC 7.0 and TRANSCompel 7.0, are accessible under http://www.gene-regulation.com/pub/databases.html...
  50. pmc Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    J Graham Ruby
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Genome Res 17:1850-64. 2007
    ..to predict miRNAs conserved among Drosophila species and large-scale sequencing of small RNAs from Drosophila melanogaster to experimentally confirm and complement these predictions...
  51. pmc RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo
    Julia Zeitlinger
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Genet 39:1512-6. 2007
    ..We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development...
  52. pmc The BioGRID Interaction Database: 2008 update
    Bobby Joe Breitkreutz
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nucleic Acids Res 36:D637-40. 2008
    ..Source code for the BioGRID and the linked Osprey network visualization system is now freely available without restriction...
  53. pmc An internal thermal sensor controlling temperature preference in Drosophila
    Fumika N Hamada
    National Center for Behavioral Genomics, Volen Center for Complex Systems, Biology Department, Brandeis University MS 008, 415 South Street, Waltham, Massachusetts 02454, USA
    Nature 454:217-20. 2008
    ..This provides a potentially general strategy for robustly selecting a narrow temperature range optimal for survival...
  54. ncbi Wolbachia and virus protection in insects
    Lauren M Hedges
    School of Integrative Biology, University of Queensland, Brisbane 4072, Australia
    Science 322:702. 2008
    ..We demonstrate a fitness benefit for Wolbachia-infected insects that may explain this paradox. Drosophila melanogaster flies infected with Wolbachia are less susceptible to mortality induced by a range of RNA viruses...
  55. pmc Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes
    Michele Markstein
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Genet 40:476-83. 2008
    ..The generality of our approach makes it adaptable to other model systems to identify and modify loci for optimal transgene expression...
  56. ncbi Application of a time-delay neural network to promoter annotation in the Drosophila melanogaster genome
    M G Reese
    Berkeley Drosophila Genome Project, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200, USA
    Comput Chem 26:51-6. 2001
    ..of a eukaryotic core promoter region has been developed and its application for analysis of the Drosophila melanogaster genome is presented...
  57. ncbi Correlates of sleep and waking in Drosophila melanogaster
    P J Shaw
    The Neurosciences Institute, 10640 John Jay Hopkins Drive, San Diego, CA 92121, USA
    Science 287:1834-7. 2000
    ..These results implicate the catabolism of monoamines in the regulation of sleep and waking in the fly and suggest that Drosophila may serve as a model system for the genetic dissection of sleep...
  58. pmc Deep annotation of Drosophila melanogaster microRNAs yields insights into their processing, modification, and emergence
    Eugene Berezikov
    Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, 3584 CT Utrecht, The Netherlands
    Genome Res 21:203-15. 2011
    ..We report here a meta-analysis of short RNA data from Drosophila melanogaster, aggregating published libraries with 76 data sets that we generated for the modENCODE project...
  59. pmc The generic genome browser: a building block for a model organism system database
    Lincoln D Stein
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11790, USA
    Genome Res 12:1599-610. 2002
    ..GBrowse is freely available under an open source license. The software, its documentation, and support are available at http://www.gmod.org...
  60. ncbi G protein-coupled receptors in Anopheles gambiae
    Catherine A Hill
    Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    Science 298:176-8. 2002
    ..expression and, along with 76 putative gustatory receptors, for their molecular evolution relative to Drosophila melanogaster. Examples of lineage-specific gene expansions were observed as well as a single instance of unusually ..
  61. pmc Layered reward signalling through octopamine and dopamine in Drosophila
    Christopher J Burke
    Department of Neurobiology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 492:433-7. 2012
    ..In addition, they reconcile previous findings with octopamine and dopamine and suggest that reinforcement systems in flies are more similar to mammals than previously thought...
  62. pmc Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9
    Xingjie Ren
    Gene Regulatory Laboratory, School of Medicine, Tsinghua University, Beijing 100084, China
    Proc Natl Acad Sci U S A 110:19012-7. 2013
    ..Here we report an effective and inexpensive method for genome DNA editing in Drosophila melanogaster whereby plasmid DNAs encoding short sgRNAs under the control of the U6b promoter are injected into ..
  63. ncbi Accessibility of the Drosophila genome discriminates PcG repression, H4K16 acetylation and replication timing
    Oliver Bell
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    Nat Struct Mol Biol 17:894-900. 2010
    ..Here, we measured genome-wide DNA accessibility in Drosophila melanogaster by combining M.SssI methylation footprinting with methylated DNA immunoprecipitation...
  64. ncbi hippo encodes a Ste-20 family protein kinase that restricts cell proliferation and promotes apoptosis in conjunction with salvador and warts
    Shian Wu
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Cell 114:445-56. 2003
    ..A human homolog of hpo completely rescues the overgrowth phenotype of Drosophila hpo mutants, suggesting that hpo might play a conserved role for growth control in mammals...
  65. ncbi A microRNA in a multiple-turnover RNAi enzyme complex
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 297:2056-60. 2002
    ..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells...
  66. ncbi Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster
    Fleur Ponton
    School of Biological Sciences, Heydon Laurence Building A08, The University of Sydney, NSW 2006, Australia
    J Insect Physiol 57:840-50. 2011
    b>Drosophila melanogaster is one of the most important genetic models and techniques such as reverse transcription quantitative real-time PCR (RT-qPCR) are being employed extensively for deciphering the genetics basis of physiological ..
  67. pmc Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary
    Colin D Malone
    Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Cell 137:522-35. 2009
    ....
  68. pmc X chromosome dosage compensation via enhanced transcriptional elongation in Drosophila
    Erica Larschan
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Nature 471:115-8. 2011
    ..Improving transcriptional output downstream of typical gene-specific controls may explain how dosage compensation can be imposed on the diverse set of genes along an entire chromosome...
  69. ncbi Evolution of supergene families associated with insecticide resistance
    Hilary Ranson
    Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
    Science 298:179-81. 2002
    ..A comparative genomic analysis with Drosophila melanogaster reveals that a considerable expansion of these supergene families has occurred in the mosquito...
  70. pmc The Toll and Imd pathways are the major regulators of the immune response in Drosophila
    Ennio De Gregorio
    Centre de Genetique Moleculaire, CNRS, F 91198 Gif sur Yvette, France
    EMBO J 21:2568-79. 2002
    ..Interestingly, we observed that some Drosophila immune-responsive genes are located in gene clusters, which often are transcriptionally co-regulated...
  71. pmc RNA polymerase is poised for activation across the genome
    Ginger W Muse
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Nat Genet 39:1507-11. 2007
    ..importance of polymerase stalling for transcription regulation, we carried out a genome-wide search for Drosophila melanogaster genes with Pol II stalled within the promoter-proximal region...
  72. ncbi GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets
    Joerg E Braun
    Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Mol Cell 44:120-33. 2011
    ....
  73. ncbi Combinatorial binding predicts spatio-temporal cis-regulatory activity
    Robert P Zinzen
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Nature 462:65-70. 2009
    ..This data-driven approach does not require previous knowledge of transcription factor sequence affinity, function or expression, making it widely applicable...
  74. ncbi Polycomb-dependent regulatory contacts between distant Hox loci in Drosophila
    Frédéric Bantignies
    Institut de Genetique Humaine, CNRS UPR 1142, Montpellier, France
    Cell 144:214-26. 2011
    In Drosophila melanogaster, Hox genes are organized in an anterior and a posterior cluster, called Antennapedia complex and bithorax complex, located on the same chromosome arm and separated by 10 Mb of DNA...
  75. pmc An endogenous small interfering RNA pathway in Drosophila
    Benjamin Czech
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:798-802. 2008
    ..These observations expand the repertoire of small RNAs in Drosophila, adding a class that blurs distinctions based on known biogenesis mechanisms and functional roles...
  76. pmc A high-sugar diet produces obesity and insulin resistance in wild-type Drosophila
    Laura Palanker Musselman
    Division of Endocrinology, Metabolism, and Lipid Research, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Dis Model Mech 4:842-9. 2011
    ..To gain insights into how dietary excess affects insulin resistance, we studied the simple model organism Drosophila melanogaster. Larvae reared on a high-sugar diet were hyperglycemic, insulin resistant and accumulated fat--hallmarks ..
  77. pmc The regulatory activity of microRNA* species has substantial influence on microRNA and 3' UTR evolution
    Katsutomo Okamura
    Sloan Kettering Institute, Department of Developmental Biology, 521 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, New York 10065, USA
    Nat Struct Mol Biol 15:354-63. 2008
    ..Here we show that, although Drosophila melanogaster miRNA* species are less abundant than their partners, they are often present at physiologically relevant ..
  78. ncbi Remote control of insulin secretion by fat cells in Drosophila
    Charles Geminard
    Institute for Developmental Biology and Cancer, CNRS University of Nice Sophia Antipolis, Parc Valrose, 06108 Nice, France
    Cell Metab 10:199-207. 2009
    ..Thus, the availability of nutrients is remotely sensed in fat body cells and conveyed to the brain IPCs by a humoral signal controlling ILP release...
  79. ncbi Drosophila OBP LUSH is required for activity of pheromone-sensitive neurons
    Pingxi Xu
    Department of Pharmacology, Center for Basic Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75335, USA
    Neuron 45:193-200. 2005
    ..These studies directly link pheromone-induced behavior with OBP-dependent activation of a subset of olfactory neurons...
  80. ncbi Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function
    Eric Lecuyer
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada
    Cell 131:174-87. 2007
    ..A searchable web resource documenting mRNA expression and localization dynamics has been established and will serve as an invaluable tool for dissecting localization mechanisms and for predicting gene functions and interactions...
  81. ncbi Insect olfactory receptors are heteromeric ligand-gated ion channels
    Koji Sato
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
    Nature 452:1002-6. 2008
    ..The ligand-gated ion channel formed by an insect OR complex seems to be the basis for a unique strategy that insects have acquired to respond to the olfactory environment...
  82. ncbi Sirtuin 2 inhibitors rescue alpha-synuclein-mediated toxicity in models of Parkinson's disease
    Tiago Fleming Outeiro
    Alzheimer s Research Unit, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129, USA
    Science 317:516-9. 2007
    ..Furthermore, the inhibitors protected against dopaminergic cell death both in vitro and in a Drosophila model of Parkinson's disease. The results suggest a link between neurodegeneration and aging...
  83. pmc Systems genetics of complex traits in Drosophila melanogaster
    Julien F Ayroles
    Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA
    Nat Genet 41:299-307. 2009
    ..Overlapping transcripts in modules associated with different traits provide insight into the molecular basis of pleiotropy between complex traits...
  84. ncbi The endocrine regulation of aging by insulin-like signals
    Marc Tatar
    Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, USA
    Science 299:1346-51. 2003
    ....
  85. ncbi Rest in Drosophila is a sleep-like state
    J C Hendricks
    Center for Sleep and Respiratory Neurobiology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    Neuron 25:129-38. 2000
    To facilitate the genetic study of sleep, we documented that rest behavior in Drosophila melanogaster is a sleep-like state...
  86. pmc A microRNA imparts robustness against environmental fluctuation during development
    Xin Li
    Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Drive, Northwestern University, Evanston, Illinois 60208, USA
    Cell 137:273-82. 2009
    ..We suggest that some conserved microRNAs like miR-7 may enter into novel genetic relationships to buffer developmental programs against variation and impart robustness to diverse regulatory networks...
  87. ncbi Improved splice site detection in Genie
    M G Reese
    Human Genome Informatics Group, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    J Comput Biol 4:311-23. 1997
    ..In further splice site experiments, we also looked at correlations between splice site scores and intron and exon lengths, as well as at the effect of distance to the nearest splice site on false positive rates...
  88. ncbi The shaping of male courtship posture by lateralized gustatory inputs to male-specific interneurons
    Masayuki Koganezawa
    Division of Neurogenetics, Tohoku University Graduate School of Life Sciences, Sendai 980 8578, Japan
    Curr Biol 20:1-8. 2010
    Unilateral wing vibration to generate "love songs" is a hallmark of male courtship posture in Drosophila melanogaster. In contrast, males of some other Drosophila species extend both wings simultaneously during courtship...
  89. ncbi Genome-wide analysis of a long-term evolution experiment with Drosophila
    Molly K Burke
    Ecology and Evolutionary Biology, University of California, Irvine, 321 Steinhaus Hall, Irvine, California 92697 2525, USA
    Nature 467:587-90. 2010
    ..Here we present whole-genome resequencing data from Drosophila melanogaster populations that have experienced over 600 generations of laboratory selection for accelerated ..
  90. pmc Regulation of Drosophila lifespan by JNK signaling
    Benoit Biteau
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Exp Gerontol 46:349-54. 2011
    ..Here, we review recent studies in Drosophila that elucidate the tissue-specific and systemic consequences of JNK activation that ultimately impact lifespan of the organism...
  91. pmc Functional genomic screen reveals genes involved in lipid-droplet formation and utilization
    Yi Guo
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA
    Nature 453:657-61. 2008
    ..These phenotypes are conserved in mammalian cells, suggesting that insights from these studies are likely to be central to our understanding of human diseases involving excessive lipid storage...
  92. pmc Analysis of homeodomain specificities allows the family-wide prediction of preferred recognition sites
    Marcus B Noyes
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 133:1277-89. 2008
    ..This resource also allows prediction of homeodomain specificities from other organisms, which is demonstrated by the prediction and analysis of human homeodomain specificities...
  93. pmc Developmental roles of 21 Drosophila transcription factors are determined by quantitative differences in binding to an overlapping set of thousands of genomic regions
    Stewart MacArthur
    Genomics Division, Lawrence Berkeley National Laboratory, Cyclotron Road MS 84 181, Berkeley, CA 94720, USA
    Genome Biol 10:R80. 2009
    ....
  94. pmc Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells
    Megha Ghildiyal
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 320:1077-81. 2008
    ..in length, that correspond to transposons and heterochromatic sequences in the somatic cells of Drosophila melanogaster. We also detected endo-siRNAs complementary to messenger RNAs (mRNAs); these siRNAs disproportionately ..
  95. ncbi Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex
    D D Sarbassov
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 307:1098-101. 2005
    ..Rictor-mTOR may serve as a drug target in tumors that have lost the expression of PTEN, a tumor suppressor that opposes Akt/PKB activation...
  96. pmc The Drosophila melanogaster transcriptome by paired-end RNA sequencing
    Bryce Daines
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genome Res 21:315-24. 2011
    RNA-seq was used to generate an extensive map of the Drosophila melanogaster transcriptome by broad sampling of 10 developmental stages. In total, 142...
  97. pmc Expression pattern and, surprisingly, gene length shape codon usage in Caenorhabditis, Drosophila, and Arabidopsis
    L Duret
    Laboratoire de Biometrie, Génétique et Biologie des Populations, Unité Mixte de Recherche Centre National de la Recherche Scientifique 5558, Villeurbanne Cedex, France
    Proc Natl Acad Sci U S A 96:4482-7. 1999
    ..and analyzed codon usage in 8,133, 1,550, and 2,917 genes, respectively, from Caenorhabditis elegans, Drosophila melanogaster, and Arabidopsis thaliana...
  98. ncbi GAL4 system in Drosophila: a fly geneticist's Swiss army knife
    Joseph B Duffy
    Department of Biology, Indiana University, 1001 E 3rd Street, Bloomington, IN 47405, USA
    Genesis 34:1-15. 2002
  99. ncbi Activation of pheromone-sensitive neurons is mediated by conformational activation of pheromone-binding protein
    John D Laughlin
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, 12801 East 17th Avenue, M S 8303, P O Box 6511, Aurora, CO 80045, USA
    Cell 133:1255-65. 2008
    ..Therefore, the pheromone-binding protein is an inactive, extracellular ligand converted by pheromone molecules into an activator of pheromone-sensitive neurons and reveals a distinct paradigm for detection of odorants...
  100. ncbi A single p450 allele associated with insecticide resistance in Drosophila
    P J Daborn
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Science 297:2253-6. 2002
    ..Here, via microarray analysis of all P450s in Drosophila melanogaster, we show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a ..
  101. pmc Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1
    Janghoo Lim
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 452:713-8. 2008
    ..This model provides mechanistic insight into the molecular pathogenesis of SCA1 as well as other polyglutamine diseases...

Research Grants67

  1. Sphingolipid Metabolism in Drosophila Development
    Julie D Saba; Fiscal Year: 2013
    ..Studies supported by this grant have explored the role of sphingolipids in the model organism Drosophila melanogaster. These studies have resulted in the chemical characterization of Drosophila sphingoid bases and the ..
  2. Using Drosophila to understand WNK and SPAK/OSR1 regulation of SLC12 cotransporte
    AYLIN RACHEL RODAN; Fiscal Year: 2013
    ..D., and fellowship studies, and in particular previous experience studying Drosophila melanogaster, by developing new scientific skills in physiology and biochemistry...
  3. Novel genomic effects of Y-linked polymorphisms
    Daniel L Hartl; Fiscal Year: 2012
    ..The novelty emerges from our recent discovery that the Y chromosome of Drosophila melanogaster is polymorphic for sequences that differentially affect the expression of many hundreds of autosomal and ..
  4. Persisting Grand Mal and Myoclonic Seizures in JME including Veterans
    Antonio V Delgado-Escueta; Fiscal Year: 2013
    ..guide which putative epilepsy genes have priority for studies of functions and disease mechanisms in Drosophila melanogaster (Aim 3), mice (Aim 2) and humans (Aim 1)...
  5. Regulation of Rhox5 Transcriptional Elongation
    Marcy E Richardson; Fiscal Year: 2013
    ..Recent studies conducted in human embryonic stem cells and Drosophila melanogaster embryos have identified transcriptional elongation as a widely used alternative control mechanism of ..
  6. Analysis of the functions of axon guidance genes during vector mosquito developme
    MOLLY DUMAN SCHEEL; Fiscal Year: 2012
    ..recent years, evolutionary developmental biologists have applied knowledge of developmental genetics in Drosophila melanogaster, a genetically tractable model organism, to better understand development of other arthropods...
  7. Oxidative stress and neurogenetic networks in Drosophila
    Robert R H Anholt; Fiscal Year: 2010
    ..b>Drosophila melanogaster provides a genetically tractable model system, because the genetic background and environmental ..
  8. Genetic Control of Axial Patterning in Drosophila Eye
    Amit Singh; Fiscal Year: 2010
    ..A well established model of the Drosophila melanogaster (fruit fly) will be employed to study dorso-ventral (DV) (axial) patterning and growth in the developing ..
  9. AAA ATPases linking mitochondria with microtubule processing in flies and yeast
    Karen G Hales; Fiscal Year: 2012
    ..Many human genetic neurodegenerative disorders are associated with defects of mitochondrial dynamics. Drosophila melanogaster spermatogenesis is an ideal model system for genetic dissection of conserved mechanisms of mitochondrial ..
  10. Molecular Genetics of Caloric Restriction in Aging Flies
    Blanka Rogina; Fiscal Year: 2013
    ..Mutation in the Indy gene in the fruit fly, Drosophila melanogaster, dramatically extends life span...
  11. Is symmetrical dimethylation of Arginine 2 of Histone H3 used as an epigenetic si
    Marjorie A Oettinger; Fiscal Year: 2010
    ..Fourth, we will analyze these modifications in model organisms (S. cerevisiae, S. pombe and Drosophila melanogaster), determining both the genome-wide localization pattern of the modifications and whether they are ..
  12. Analyzing Anthrax Toxins in Drosophilia
    Ethan Bier; Fiscal Year: 2013
    ..in barrier disruption We discovered this novel effect of anthrax toxins using the model genetic system Drosophila melanogaster (fruit fly) and then validated these effects in human vascular endothelial cells as well as in vivo in ..
  13. Genome-wide Identification of Immediate Early Genes in Insects
    Anandasankar Ray; Fiscal Year: 2012
    ..few genes have been identified as IEGs in mammals, but very little is known about IEGs in insects such as Drosophila melanogaster, which is used as a model to study these biological process...
  14. EGF RECEPTOR MEDIATED SIGNALING IN DROSOPHILA
    GERTRUD M SCHUPBACH; Fiscal Year: 2013
    ..focuses on signaling between the female germline and its surrounding follicle cells in the ovary of Drosophila melanogaster. We have shown that the Drosophila Egfr is expressed in the follicle cells and receives a highly ..
  15. Insulin, IGF and Insulin Signaling: effects on Anopheles lifespan and immunity
    MICHAEL ALLEN RIEHLE; Fiscal Year: 2012
    ..impacts on mosquito physiology and vector competence that were conceptualized for Caenorhabditis elegans, Drosophila melanogaster and mammals...
  16. Regulation of TRP channels and visual transduction
    Craig Montell; Fiscal Year: 2013
    ..goal of our research is to understand the mechanisms underlying phototransduction in the fruitfly, Drosophila melanogaster. Drosophila phototransduction functions through a phospholipase C (PLC)- dependent signaling system, and ..
  17. Expression control in Drosophila splicing assembly factor RNP-4F
    JACK VAUGHN; Fiscal Year: 2010
    ..of the evolutionarily-conserved splicing assembly factor RNP-4F in the important genetic model organism Drosophila melanogaster. Specifically, we postulate that an isoform of dADAR adenosine deaminase plays a major role in a ..
  18. Genetic and Molecular Studies of Drosophila Chromatin Remodeling Factors
    John W Tamkun; Fiscal Year: 2013
    ..To address these important issues, our laboratory uses Drosophila melanogaster as a model organism to study the function of chromatin-remodeling and modifying enzymes...
  19. How do Amine Neurons Work?
    Edward A Kravitz; Fiscal Year: 2013
    ..These experimental approaches are the farthest advanced in fruit flies (Drosophila melanogaster) where powerful genetic methods allow identification and manipulation of single neurons concerned with ..
  20. Using Drosophila as a model to understand TDP-43 function in ALS
    David B Morton; Fiscal Year: 2012
    ..We are using the fruit fly, Drosophila melanogaster, as a model to understand the function of TDP- 43 in neurons and the consequences of aberrant expression...
  21. Genetic control of quantitative traits associated with the metabolic syndrome
    Maria De Luca; Fiscal Year: 2013
    ..These genetic pathways will be identified by integrating genetic and expression network analysis in Drosophila melanogaster. Using this information, human population-based association studies will be performed to investigate the ..
  22. Generation of Wunen/LPP3-based therapy for muscular dystrophy
    Hannele Ruohola Baker; Fiscal Year: 2010
    ..of LPP3 that reduces the level of active second messenger, S1P can suppress dystrophic phenotypes in Drosophila melanogaster. We will now study the mechanism of sphingolipid pathway and Dystrophin interactions and further, will ..
  23. Regulation of tubulogenesis in the Drosophila ovary
    Celeste A Berg; Fiscal Year: 2012
    ..This proposal uses Drosophila melanogaster egg chambers to investigate the mechanisms that control tube size and shape...
  24. Genetic and Molecular Mechanisms of Ethanol-Induced Developmental Defects
    RACHAEL LOUISE FRENCH; Fiscal Year: 2013
    ..and study the molecular targets of developmental ethanol using the genetically amenable model organism Drosophila melanogaster. Drosophila melanogaster, the common fruit fly, has been utilized extensively in biological research, ..
  25. Proteomics of Cell Death via 2-D Microfluidic Profiling
    DON DEVOE; Fiscal Year: 2009
    ..By using the fruit fly Drosophila melanogaster as a model system, these studies will explore pathways and identify biomarkers associated with Caspase ..
  26. A Drosophila Model for Genetic Studies of Metabolism
    Carl S Thummel; Fiscal Year: 2010
    ..The goal of our research is to use the fruit fly, Drosophila melanogaster, as a simple model system to define the central regulatory pathways that control metabolism and maintain ..
  27. Identifying novel anti-infectives by high through-put screening in whole animals
    Frederick M Ausubel; Fiscal Year: 2013
    ..in 384-well plates is followed by secondary screening in a more highly evolved model host, the fruit fly Drosophila melanogaster, increasing the likelihood of isolating drugs that will work in humans...
  28. Group II Intron Mobility and Gene Targeting
    Alan M Lambowitz; Fiscal Year: 2012
    ..group II intron-based gene targeting methods for eukaryotic model organisms, focusing initially on Drosophila melanogaster. (4) To use Xenopus tropicalis to further develop methods for using group II introns for gene targeting ..
  29. Discovery of Vibrio cholerae factors contributing to virulence in Drosophila.
    ALEXANDRA ELIZABETH PURDY; Fiscal Year: 2010
    ..Previous work in the Watnick laboratory has established Drosophila melanogaster as a model organism for V. cholerae intestinal infection...
  30. Steroid-Dependent Changes in the Yorkie Interactome
    Alexey Veraksa; Fiscal Year: 2013
    ..SWH) pathway is a well-conserved metazoan signaling cascade that restricts organ size in the fruit fly Drosophila melanogaster and limits tumorigenesis in mammals...
  31. Aging specific gene expression in Drosophila
    JOHN GERARD TOWER; Fiscal Year: 2013
    ..of this research is to understand the regulation of gene expression during aging using the model organism Drosophila melanogaster. Aging in both flies and humans is found to be associated with a tissue-specific induction of oxidative ..
  32. Octopamine Neuroanatomy and Functioning in Fruit Fly Social Behavior
    KYLE LYNN GOBROGGE; Fiscal Year: 2013
    ..The fruit fly (Drosophila melanogaster) genome is fully sequenced, and as a model system, fruit flies offer numerous possibilities for ..
  33. Developmental Regulation of the Cell Cycle in Drosophila
    LAURA ANNE LEE; Fiscal Year: 2013
    ..conservation of genes involved in the cell cycle combined with the superb genetics and cytology of Drosophila melanogaster make it an ideal model organism for studying cell-cycle regulation in a developmental context...
  34. Drosophila feeding behavior: New tools for the genetic dissection of prandiology
    William W Ja; Fiscal Year: 2012
    ..The fruit fly, Drosophila melanogaster, is a powerful system for studying the relationship of genes with behavior and disease...
  35. Hormonal Modulation of Aggression in Drosophila
    Edward A Kravitz; Fiscal Year: 2013
    ..Over the past decade, we have developed a Drosophila melanogaster model of aggression...
  36. Nuclear mechanics and mechanotransduction in muscular laminopathies
    Jan Lammerding; Fiscal Year: 2013
    ..Using drosophila melanogaster models expressing a panel of lamin mutations with variable muscle involvement, we will relate effects of ..
  37. Interplay between the Endocrine and Innate Systems of Drosphila
    Neal Silverman; Fiscal Year: 2013
    ..This proposal uses the fruit fly Drosophila melanogaster as a model for the study of these interactions...
  38. A model of kidney stone disease using D. melanogaster
    Marshall Stoller; Fiscal Year: 2013
    ..In Drosophila melanogaster (D...
  39. Regulation of RNA Surveillance
    MILES FROME WILKINSON; Fiscal Year: 2012
    ..analyses from several groups indicate that between 5 and 10% of the genes in Saccharomyces cerevisiae, Drosophila melanogaster, and mammals give rise to transcripts that are subject to NMD...
  40. Developing cell lines from clock neurons in Drosophila
    Paul E Hardin; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The identification and analysis of clock genes in the fruit fly, Drosophila melanogaster, revealed that the circadian clock is based on autoregulatory feedback loops in gene expression...
  41. Taste and feeding circuits in Drosophila
    MICHAEL DOUGLAS GORDON; Fiscal Year: 2010
    ..proposal will examine the neural substrates of taste perception and feeding behavior in the fruit fly Drosophila melanogaster. Flies offer an excellent model system for studying this problem because they exhibit robust behavioral ..
  42. Function of the Six and Eya Genes in Retinal Development
    Justin P Kumar; Fiscal Year: 2012
    ..patients and mouse model systems as well as total elimination of the compound eyes of the fruit fly, Drosophila melanogaster. The association of Six and Eya gene lesions with retinal disorders in both insect and mammalian systems ..
  43. Role of Myc in the Growth Regulation Pathways
    PAOLA BELLOSTA BELLOSTA; Fiscal Year: 2010
    ..In our laboratory we are using the fruitfly Drosophila melanogaster as an animal model to study the processes that regulate these diseases...
  44. Small RNA and whole chromosome recognition in Drosophila melanogaster.
    Victoria H Meller; Fiscal Year: 2013
    ..The similarities between analogous processes in flies and mammals suggest that this mechanism may also contribute to normal genome regulation in humans. ..
  45. Mobilization of Triacylglycerol Stores in Insects
    Estela L Arrese; Fiscal Year: 2012
    ..The main lipase found in the insect fat body, TG-lipase (TGL) homolog to Drosophila melanogaster CG8552, and a lipid droplet-associated protein, Lsd1, whose phosphorylation stimulates the rate of ..
  46. The epigenetic basis of cytoplasmic incompatibility
    KRISTIN KALIE JERNIGAN; Fiscal Year: 2012
    ..iii) Third, our preliminary evidence shows that infected Drosophila melanogaster flies treated with a small molecule inhibitor of DNA methylation (5-Azacytidine) exhibit CI when only one ..
  47. Genetics and Molecular Biology of Striated Muscle Myosin
    Sanford I Bernstein; Fiscal Year: 2013
    ..will employ a multidisciplinary approach that takes advantage of the powerful genetic tools available for Drosophila melanogaster along with a broad range of expertise that allows us to study myosin in an integrative manner, from its ..
  48. Ecdysone modulation of sexual behavior in Drosophila melanogaster
    GEOFFREY GANTER; Fiscal Year: 2009
    We will explore the control of sexual behavior of the fruit fly by the steroid hormone ecdysone. The Drosophila melanogaster model system has produced a quantity of data relevant to higher functions of the human nervous system, ..
  49. Cellular Biomechanics of Heat-Shock Induced Defects in Drosophila Embryogenesis
    M Shane Hutson; Fiscal Year: 2013
    ..The project focuses on similar effects in the model system of Drosophila melanogaster in which the developmental defects have been termed phenocopies for their ability to mimic the phenotypes ..
  50. IDENTIFICATION OF GENES ASSOCIATED WITH MYOBLAST FUSION
    Susan M Abmayr; Fiscal Year: 2012
    ..We have chosen embryonic development of the larval body wall muscles in the fruitfly Drosophila melanogaster as a model system, in an effort to utilize the advantages of a combined genetic and molecular approach...
  51. Neuronal Cell Cycle and Survival
    Nicholas E Baker; Fiscal Year: 2013
    ..The eye of the fruit fly Drosophila melanogaster will be used to evaluate this new hypothesis, and to identify specific aspects of cell cycle progression, ..
  52. A Drosophila Model for Williams Syndrome Cognitive Processing
    Ralph J Greenspan; Fiscal Year: 2010
    ..This proposal outlines a plan to develop a behavioral/genetic model in Drosophila melanogaster to study the distinctive cognitive phenotype affecting visuospatial processing found in humans with ..
  53. Function of the Six and Eya Genes in Retinal Development
    JUSTIN KUMAR; Fiscal Year: 2009
    ..patients and mouse model systems as well as total elimination of the compound eyes of the fruit fly, Drosophila melanogaster. The association of Six and Eya gene lesions with retinal disorders in both insect and mammalian systems ..
  54. Variable Dose Rate X-ray Irradiator
    Howard B Lieberman; Fiscal Year: 2010
    ..ideal to enable studies to be undertaken on a wide array of model systems such as radioresistant yeast, Drosophila melanogaster and Caenorhabditis elegans, as well as mammalian cells in culture, or small rodents. Drs...
  55. Deciphering the Composition of oskar mRNP via in vivo Fluorescence Imaging
    DIANA BRATU; Fiscal Year: 2010
    ..is to examine the spatio-temporal requirements of trans-acting factors during mRNA transport in Drosophila melanogaster oocytes...
  56. Role of circadian clocks in maintaining a healthy nervous system
    SVETLANA NIKOLAEVNA RADYUK; Fiscal Year: 2012
    ..To test this hypothesis, we propose an interdisciplinary collaboration using the excellent model system Drosophila melanogaster. In aim 1, we will determine roles of circadian clocks in the regulation of glutathione biosynthesis in ..
  57. Genetic analysis and regulation of amyloids flux in the Drosophila heart
    GIRISH CHANDRA MELKANI; Fiscal Year: 2011
    ..To test our hypothesis, we will use the genetically tractable model organism Drosophila melanogaster and employ an integrative approach in exploration and suppression of cardiac amyloidosis...
  58. Evolution of Drosophila melanogaster cis-regulatory modules
    Aaron Hardin; Fiscal Year: 2013
    ..I use as a model early anterior-posterior patterning in Drosophila melanogaster and its relatives...
  59. L2DTL Function in Maintaining Genomic Stability during Drosophila Development
    CATHERINE SILVER KEY; Fiscal Year: 2010
    ..Since Cdt1/Dup has been extensively studied in Drosophila in various cell and development contexts, Drosophila melanogaster serves as an excellent model for elucidating the role of the L2DTL family in regulating Cdt1/Dup levels ..
  60. Role of FGF Signaling in Controlling Cell Movements During Drosophila Development
    ANGELIKE M STATHOPOULOS; Fiscal Year: 2013
    ..We have found that only three of six possible FGF-FGFR interactions are acting in Drosophila melanogaster: Pyramus and Thisbe FGF ligands each support activation of the FGFR Heartless, while only the FGF ligand ..
  61. Characterization of a mutation that affects feeding and digestion in Drosophila
    EDWARD BLUMENTHAL; Fiscal Year: 2009
    ..and physiological pathways required for the regulation of digestion and feeding behavior in the fruit fly Drosophila melanogaster, an organism that has proven to be an excellent model system for studies of feeding regulation and energy ..
  62. Activation of Insect Immunity by Gram-negative Bacteria
    Neal Silverman; Fiscal Year: 2013
    ..This proposal uses the fruit fly Drosophila melanogaster as a model for the study of innate immunity...
  63. CRYOPRESERVATION OF DROSOPHILA MELANOGASTER EMBRYOS
    PETER STEPONKUS; Fiscal Year: 1993
    The ultimate goal of this project is to develop a protocol for the cryopreservtion of Drosophila melanogaster embryos. We have investigated three different strategies: conventional cryopreservation, vitrification, and supercooling...
  64. TEST OF NEUTRAL MOLECULAR EVOLUTION
    Martin Kreitman; Fiscal Year: 1999
    ..are proposed; all are based on the collection of DNA sequence data from the closely related species Drosophila melanogaster and D. simulans, as well as the species pairs D. yakuba - D. teissieri and D. erecta - D. orena...
  65. EFFECTS OF AGING ON GERMLINE AND SOMATIC STEM CELLS IN DROSOPHILA MELANOGASTER
    MATTHEW WALLENFANG; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  66. Mapping and rescuing EMS-induced lethal mutations on the Drosophila X Chromosome
    Hugo J Bellen; Fiscal Year: 2010
    ..to map and deposit a collection of well defined chemically induced mutations in essential genes on the Drosophila melanogaster X chromosome for distribution by the Bloomington Drosophila Stock Center (BDSC) to the fly community...
  67. Drosophila melanogaster as a model host for Vibrio cholerae
    Paula I Watnick; Fiscal Year: 2010
    ..We have previously shown that ingestion of pathogenic strains of V. cholerae by Drosophila melanogaster results in lethal infection...