hirudo medicinalis

Summary

Summary: A species of European freshwater LEECHES used for BLOODLETTING in ancient times and also for LEECHING in modern times.

Top Publications

  1. ncbi Beyond bloodletting: FDA gives leeches a medical makeover
    Carol Rados
    FDA Consum 38:9. 2004
  2. ncbi Involvement of nitric oxide through endocannabinoids release in microglia activation during the course of CNS regeneration in the medicinal leech
    Karim Arafah
    Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée FABMS, EA 4550, Universite Lille 1, Cite Scientifique, Villeneuve d Ascq F 59655, France
    Glia 61:636-49. 2013
  3. ncbi Evidence for a novel chemotactic C1q domain-containing factor in the leech nerve cord
    Muriel Tahtouh
    Université de Lille 1 USTL, Laboratoire de Neuroimmunologie des Annelides, Centre National de la Recherche Scientifique, FRE 2933, IFR 147, F59655 Villeneuve d Ascq, France
    Mol Immunol 46:523-31. 2009
  4. ncbi A homologous form of human interleukin 16 is implicated in microglia recruitment following nervous system injury in leech Hirudo medicinalis
    Françoise Croq
    Universite Lille Nord de France, Universite Lille 1, Laboratoire de Neuroimmunologie des Annelides, Centre National de la Recherche Scientifique, FRE 3249, IFR 147, F59655 Villeneuve d Ascq, France
    Glia 58:1649-62. 2010
  5. ncbi Small bite, large impact-saliva and salivary molecules in the medicinal leech, Hirudo medicinalis
    Jan Peter Hildebrandt
    Animal Physiology and Biochemistry, Zoological Institute, Ernst Moritz Arndt University, Biotechnikum, Walther Rathenau Strasse 49 a, 17489, Greifswald, Germany
    Naturwissenschaften 98:995-1008. 2011
  6. pmc First isolation of a giant virus from wild Hirudo medicinalis leech: Mimiviridae isolation in Hirudo medicinalis
    Mondher Boughalmi
    Aix Marseille Univ, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes URMITE, UM63 CNRS 7278 IRD 198 INSERM U1095, Facultes de Medecine et de Pharmacie, Marseille, France
    Viruses 5:2920-30. 2013
  7. ncbi Functional screening of serine protease inhibitors in the medical leech Hirudo medicinalis monitored by intensity fading MALDI-TOF MS
    Oscar Yanes
    Institut de Biotecnologia i de Biomedicina and Departament de Bioquímica, Universitat Autonoma de Barcelona, 08193 Bellaterra Barcelona, Spain
    Mol Cell Proteomics 4:1602-13. 2005
  8. ncbi Up-regulation of neurohemerythrin expression in the central nervous system of the medicinal leech, Hirudo medicinalis, following septic injury
    David Vergote
    Laboratoire de Neuroimmunologie des Annelides, UMR 8017 CNRS, IFR 17 INSERM, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq, France
    J Biol Chem 279:43828-37. 2004
  9. ncbi Microtargeted gene silencing and ectopic expression in live embryos using biolistic delivery with a pneumatic capillary gun
    Orit Shefi
    Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0376, USA
    J Neurosci 26:6119-23. 2006
  10. ncbi The multifunctional role of fibroblasts during wound healing in Hirudo medicinalis (Annelida, Hirudinea)
    Gianluca Tettamanti
    Department of Structural and Functional Biology, University of Insubria Via J H Dunant 3, 21100 Varese, Italy
    Biol Cell 96:443-55. 2004

Research Grants

  1. TRANSPORT AND SORTING OF NEURONAL MEMBRANE PROTEINS
    RICHARD ROTUNDO; Fiscal Year: 1990
  2. NAVIGATION AND GUIDANCE--IDENTIFIED GROWTH CONE ARRAY
    John Jellies; Fiscal Year: 1991
  3. TRANSPORT AND SORTNG OF NEURONAL MEMBRANE PROTEINS
    RICHARD ROTUNDO; Fiscal Year: 1992
  4. HOMEBOX GENES AND NEURONAL DIFFERENTIATION
    GABRIEL AISEMBERG; Fiscal Year: 2002
  5. NEURONAL REGULATION OF BEHAVIOR
    WOLFGANG FRIESEN; Fiscal Year: 1992
  6. Receptor Phosphatases and Process Outgrowth
    Eduardo Macagno; Fiscal Year: 2005

Detail Information

Publications211 found, 100 shown here

  1. ncbi Beyond bloodletting: FDA gives leeches a medical makeover
    Carol Rados
    FDA Consum 38:9. 2004
  2. ncbi Involvement of nitric oxide through endocannabinoids release in microglia activation during the course of CNS regeneration in the medicinal leech
    Karim Arafah
    Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée FABMS, EA 4550, Universite Lille 1, Cite Scientifique, Villeneuve d Ascq F 59655, France
    Glia 61:636-49. 2013
    ..These events can bring explanations about the ability of the leech CNS to regenerate after a trauma and the key role of endocannabinoids in this phenomenon...
  3. ncbi Evidence for a novel chemotactic C1q domain-containing factor in the leech nerve cord
    Muriel Tahtouh
    Université de Lille 1 USTL, Laboratoire de Neuroimmunologie des Annelides, Centre National de la Recherche Scientifique, FRE 2933, IFR 147, F59655 Villeneuve d Ascq, France
    Mol Immunol 46:523-31. 2009
    ..In the medicinal leech Hirudo medicinalis, the recruitment of microglial cells at the lesion site is essential for sprouting of injured axons...
  4. ncbi A homologous form of human interleukin 16 is implicated in microglia recruitment following nervous system injury in leech Hirudo medicinalis
    Françoise Croq
    Universite Lille Nord de France, Universite Lille 1, Laboratoire de Neuroimmunologie des Annelides, Centre National de la Recherche Scientifique, FRE 3249, IFR 147, F59655 Villeneuve d Ascq, France
    Glia 58:1649-62. 2010
    In contrast to mammals, the medicinal leech Hirudo medicinalis can completely repair its central nervous system (CNS) after injury...
  5. ncbi Small bite, large impact-saliva and salivary molecules in the medicinal leech, Hirudo medicinalis
    Jan Peter Hildebrandt
    Animal Physiology and Biochemistry, Zoological Institute, Ernst Moritz Arndt University, Biotechnikum, Walther Rathenau Strasse 49 a, 17489, Greifswald, Germany
    Naturwissenschaften 98:995-1008. 2011
    ..Accordingly, one of the most prominent species has been named Hirudo medicinalis by Carl Linne in 1758...
  6. pmc First isolation of a giant virus from wild Hirudo medicinalis leech: Mimiviridae isolation in Hirudo medicinalis
    Mondher Boughalmi
    Aix Marseille Univ, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes URMITE, UM63 CNRS 7278 IRD 198 INSERM U1095, Facultes de Medecine et de Pharmacie, Marseille, France
    Viruses 5:2920-30. 2013
    ..We screened leeches from the species Hirudo medicinalis for giant viruses. We analyzed five H. medicinalis obtained from Tunisia (3) and France (2)...
  7. ncbi Functional screening of serine protease inhibitors in the medical leech Hirudo medicinalis monitored by intensity fading MALDI-TOF MS
    Oscar Yanes
    Institut de Biotecnologia i de Biomedicina and Departament de Bioquímica, Universitat Autonoma de Barcelona, 08193 Bellaterra Barcelona, Spain
    Mol Cell Proteomics 4:1602-13. 2005
    ..In this work, we focused our attention on the leech Hirudo medicinalis. We performed, by "intensity fading" MALDI-TOF mass spectrometry, a comprehensive detection and ..
  8. ncbi Up-regulation of neurohemerythrin expression in the central nervous system of the medicinal leech, Hirudo medicinalis, following septic injury
    David Vergote
    Laboratoire de Neuroimmunologie des Annelides, UMR 8017 CNRS, IFR 17 INSERM, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq, France
    J Biol Chem 279:43828-37. 2004
    We report here some results of a proteomic analysis of changes in protein expression in the leech Hirudo medicinalis in response to septic injury...
  9. ncbi Microtargeted gene silencing and ectopic expression in live embryos using biolistic delivery with a pneumatic capillary gun
    Orit Shefi
    Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0376, USA
    J Neurosci 26:6119-23. 2006
  10. ncbi The multifunctional role of fibroblasts during wound healing in Hirudo medicinalis (Annelida, Hirudinea)
    Gianluca Tettamanti
    Department of Structural and Functional Biology, University of Insubria Via J H Dunant 3, 21100 Varese, Italy
    Biol Cell 96:443-55. 2004
    ....
  11. ncbi Hirudo medicinalis: a platform for investigating genes in neural repair
    W Z Wang
    Department of Human Anatomy and Genetics, South Parks Road, University of Oxford OX1 3QX, UK
    Cell Mol Neurobiol 25:427-40. 2005
    ..It enables us to determine the time course of gene expression in the regenerating nerve cord, and to study the effects of gene knockdown in identified neurons regenerating in defined conditions in culture...
  12. pmc Microbial challenge promotes the regenerative process of the injured central nervous system of the medicinal leech by inducing the synthesis of antimicrobial peptides in neurons and microglia
    David Schikorski
    Centre National de la Recherche Scientifique FRE2933, Laboratoire de Neuroimmunologie des Annelides, Universite de Lille 1, Villeneuve d Ascq, France
    J Immunol 181:1083-95. 2008
    ..Thus, the leech CNS appears as an excellent model for studying the implication of immune molecules in neural repair...
  13. ncbi Deciphering the immune function and regulation by a TLR of the cytokine EMAPII in the lesioned central nervous system using a leech model
    David Schikorski
    Laboratoire de Neuroimmunologie des Annelides, FRE Centre National de la Recherche Scientifique 2933, Groupe Signaux de Danger, Voies de Signalisation et Effecteurs, Universite de Lille 1, 59655 Villeneuve d Ascq, France
    J Immunol 183:7119-28. 2009
    ..p43/endothelial monocyte-activating polypeptide II (EMAPII) was characterized in the CNS of the leech Hirudo medicinalis. As observed in mammals, the leech complex is processed to release the cytokine HmEMAPII...
  14. ncbi [Hirudo medicinalis-leech applications in plastic and reconstructive microsurgery--a literature review]
    K Knobloch
    Plastische, Hand und Wiederherstellungschirurgie, Medizinische Hochschule Hannover, Carl Neuberg Strasse 1, 30625 Hannover
    Handchir Mikrochir Plast Chir 39:103-7. 2007
    ..Especially venous congestion is decreased using hirudo medicinalis application such as following replantation of amputated fingers or congested flaps...
  15. ncbi Characterizations of Hirudo medicinalis DNA promoters for targeted gene expression
    Michael W Baker
    Section of Cell and Developmental Biology, Division of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    J Neurosci Methods 156:145-53. 2006
    ..We report here on the isolation and functional analysis of three Hirudo medicinalis actin gene promoters and the 5' UTR of a leech elongation factor-1alpha (HmEF-1alpha) gene...
  16. ncbi [Leech hirudo medicinalis: a therapeutic alternative available in Mexico]
    Agustín Cornejo-Esquerra
    Centro Medico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico
    Rev Med Inst Mex Seguro Soc 47:335-40. 2009
    Throughout history a diversity of therapeutic properties have been attributed to the leech Hirudo medicinalis, however, during the past twenty years its use has proven to be a practical and effective method for the relief of venous ..
  17. ncbi [Polyfunctionality of destabilase, a lysozyme from a medicinal leech]
    I P Baskova
    Bioorg Khim 34:337-43. 2008
    ..The ability of the enzyme to hydrolyze endoisopeptide bonds formed by transglutaminases, which are detected in many pathological conditions, including thrombosis, is considered from the viewpoint of its further application in practice...
  18. ncbi Activity-dependent suppression of spontaneous spike generation in the Retzius neurons of the leech Hirudo medicinalis L
    Tobias Rose
    Institute for Zoology and Anthropology, Georg August Universitat Gottingen, Berlinerstrasse 28, 37073, Gottingen, Germany
    Invert Neurosci 6:169-76. 2006
    ....
  19. pmc Construction of a medicinal leech transcriptome database and its application to the identification of leech homologs of neural and innate immune genes
    Eduardo R Macagno
    Division of Biological Sciences, University of California, San Diego, CA, USA
    BMC Genomics 11:407. 2010
    The medicinal leech, Hirudo medicinalis, is an important model system for the study of nervous system structure, function, development, regeneration and repair...
  20. ncbi Antibacterial non-glycosidase activity of invertebrate destabilase-lysozyme and of its helical amphipathic peptides
    L L Zavalova
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
    Chemotherapy 52:158-60. 2006
    Since bactericidal properties of some lysozymes are independent of their glycosidase activity, we have investigated this phenomenon for destabilase-lysozyme (DL) from medicinal leech (Hirudo medicinalis).
  21. ncbi Imaging dedicated and multifunctional neural circuits generating distinct behaviors
    Kevin L Briggman
    Department of Biology, University of California, San Diego, La Jolla, California 92037 0357, USA
    J Neurosci 26:10925-33. 2006
    ..These results show that the swimming and crawling networks are driven by both multifunctional and dedicated circuitry...
  22. ncbi 5-Hydroxytryptamine-mediated increase in glutamate uptake by the leech giant glial cell
    Ingolf C Hirth
    Abteilung fur Allgemeine Zoologie, FB Biologie, Universitat Kaiserslautern, Postfach 3049, D 67653 Kaiserslautern, Germany
    Glia 54:786-94. 2006
    ..The activity of the glutamate uptake carrier in the giant glial cell in isolated ganglia of Hirudo medicinalis was monitored by measuring the membrane current and the change in the intracellular Na(+) concentration (Na(+..
  23. ncbi Spike-mediated and graded inhibitory synaptic transmission between leech interneurons: evidence for shared release sites
    Andrei I Ivanov
    Department of Biology, Emory University, Atlanta, GA 30322, USA
    J Neurophysiol 96:235-51. 2006
    ..The data argue for a closer location of HVA Ca channels to release sites than LVA Ca channels. The results are summarized in a conceptual model of a heart interneuron release site...
  24. ncbi Role for calcium signaling and arachidonic acid metabolites in the activity-dependent increase of AHP amplitude in leech T sensory neurons
    Rossana Scuri
    Department of Physiology and Biochemistry G Moruzzi, University of Pisa, Pisa, Italy
    J Neurophysiol 94:1066-73. 2005
    ..of activity-dependent modulation of the afterhyperpolarization (AHP) in tactile (T) neurons of the leech Hirudo medicinalis. The firing of T cells is characterized by an AHP, which is mainly due to the activity of the Na+/K+ ATPase...
  25. ncbi Postactivation inhibition of spontaneously active neurosecretory neurons in the medicinal leech
    Daniela Gocht
    Department of Neurobiology, Institute of Zoology, Berliner Strasse 28, 37073, Gottingen, Germany
    J Comp Physiol A Neuroethol Sens Neural Behav Physiol 193:347-61. 2007
    ..activation of spontaneously active serotonin-containing Retzius cells in isolated ganglia of the leech Hirudo medicinalis induced prolonged membrane hyperpolarisations causing periods of postactivation inhibition of up to 33 s...
  26. ncbi Mg2+-malate co-transport, a mechanism for Na+-independent Mg2+ transport in neurons of the leech Hirudo medicinalis
    Dorothee Gunzel
    Institut für Klinische Physiologie, Heinrich Heine Universitat Dusseldorf, Germany
    J Neurophysiol 94:441-53. 2005
    Mg2+-extrusion from Mg2+-loaded neurons of the leech, Hirudo medicinalis, is mediated mainly by Na+/Mg2+ antiport...
  27. ncbi Sensitization and dishabituation of swim induction in the leech Hirudo medicinalis: role of serotonin and cyclic AMP
    Maria Luisa Zaccardi
    Dipartimento di Fisiologia e Biochimica G Moruzzi, Universita degli Studi di Pisa, Via S Zeno 31, 56127 Pisa, Italy
    Behav Brain Res 153:317-26. 2004
    ..AMP (cAMP) in sensitization and dishabituation of swim induction (SI) has been investigated in the leech Hirudo medicinalis. Electrical stimulation of the body wall evokes swimming activity with a constant latency...
  28. ncbi Graded inhibitory synaptic transmission between leech interneurons: assessing the roles of two kinetically distinct low-threshold Ca currents
    Andrei I Ivanov
    Department of Biology, Emory University, Atlanta, GA 30322, USA
    J Neurophysiol 96:218-34. 2006
    ....
  29. pmc Neuronal competition for action potential initiation sites in a circuit controlling simple learning
    G E Cruz
    Department of Physiology and Biophysics, University of Miami School of Medicine, P O Box 016430 R 430, Miami, FL 33136, USA
    Neuroscience 148:65-81. 2007
    ..In one mode, inputs to different sites sum independently, whereas in another, synaptic input to a single site precisely specifies the overall pattern of activity...
  30. ncbi Functions of the subesophageal ganglion in the medicinal leech revealed by ablation of neuromeres in embryos
    Andrea Cornford
    Division of Biological Sciences, Neurobiology Section, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0357, USA
    J Exp Biol 209:493-503. 2006
    ..These roles of the leech cephalic brain are common features of brain function in many organisms, and our results are consistent with the hypothesis that they arose early in evolution and have been conserved in complex nervous systems...
  31. pmc Co-induction of long-term potentiation and long-term depression at a central synapse in the leech
    Brian D Burrell
    Neuroscience Group, Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, 414 E Clark St, Vermillion, SD 57069, USA
    Neurobiol Learn Mem 90:275-9. 2008
    ..This co-induction of LTP and LTD likely plays an important role in activity-dependent regulation of synaptic transmission...
  32. ncbi Three-dimensional culture of leech and snail ganglia for studies of neural repair
    E J Babington
    Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK
    Invert Neurosci 5:173-82. 2005
    ....
  33. ncbi G protein activation by uncaging of GTP-gamma-S in the leech giant glial cell
    Ingo C Hirth
    Abteilung fur Allgemeine Zoologie, FB Biologie, TU Kaiserslautern, Kaiserslautern, Germany
    J Exp Biol 210:3771-9. 2007
    ..conductance, intracellular Ca(2+) and Na(+) of the giant glial cell in isolated ganglia of the leech Hirudo medicinalis. Uncaging GTP-gamma-S (injected into a giant glial cell as caged compound) by moderate UV illumination ..
  34. pmc Forgotten digital tourniquet: salvage of an ischaemic finger by application of medicinal leeches
    C Durrant
    Wexham Park Hospital, Slough, Berkshire, UK
    Ann R Coll Surg Engl 88:462-4. 2006
    ..We present one case where a digit was salvaged after 4 days of tourniquet application, using medicinal leeches...
  35. ncbi [Effect of the medicinal leech salivary gland secretion on the state of rat subcutaneous mast cells]
    I P Baskova
    Ross Fiziol Zh Im I M Sechenova 91:195-207. 2005
    ....
  36. ncbi Suppurative sequelae of symbiosis
    Andrew Steer
    Paediatric Infectious Diseases Unit, Department of General Medicine, University of Melbourne, Australia
    Lancet 365:188. 2005
  37. pmc Bleeding due to a medicinal leech bite
    I Ikizceli
    Department of Emergency Medicine, Erciyes University School of Medicine, Kayseri, Turkey
    Emerg Med J 22:458-60. 2005
    ..The paper discusses the characteristics of the wounds and possible complication of prolonged bleeding following medicinal leech application. The principles of treatment are also described...
  38. doi Medicinal leech fixation in precarious locations
    Jonathan Bank
    J Reconstr Microsurg 24:67-8. 2008
  39. ncbi The medicinal leech
    Maxwell Urata
    HMSA, USA
    Hawaii Med J 65:9, 11. 2006
  40. ncbi Study of regular intracellular and membrane processes in neurons by laser interference microscopy
    L A Erokhova
    Biological Faculty, M V Lomonosov Moscow State University, Moscow
    Bull Exp Biol Med 140:262-4. 2005
    ..The frequency spectra for the central and pri-membrane regions of the cells are different...
  41. ncbi Development of swimming in the medicinal leech, the gradual acquisition of a behavior
    K A French
    Division of Biological Sciences, Neurobiology Section, UCSD, 9500 Gilman Dr, La Jolla, CA 92093 0357, USA
    J Comp Physiol A Neuroethol Sens Neural Behav Physiol 191:813-21. 2005
    ..These kinematic details suggest how the swim central pattern generating circuit is assembled during embryogenesis...
  42. ncbi [Steroids, histamine and serotonin in medicinal leech salivary gland secretions]
    I P Baskova
    Biomed Khim 54:127-39. 2008
    ..Using standart preparations as the reference steroid hormones (cortisol, dehydroepiandrosterone, androstenedione and testosterone) and histamine and serotonine have been identified in SGS...
  43. ncbi [Blood serum of patients with secondary progressive multiple sclerosis changes neuron electric activity]
    S S Sergeeva
    Zh Nevrol Psikhiatr Im S S Korsakova 105:30-4. 2005
    ..of 20% blood serum estimated by the changes of background and excited spike activity of Retzius' neuron by Hirudo medicinalis, which does not contain myelin, has been studied in 2 groups of patients...
  44. ncbi A new species of the medicinal leech (Oligochaeta, Hirudinida, Hirudo) from Transcaucasia and an identification key for the genus Hirudo
    Serge Y Utevsky
    Department of Zoology and Animal Ecology, V N Karazin Kharkiv National University, Kharkiv 61077, Ukraine
    Parasitol Res 98:61-6. 2005
    ..contains a neglected species previously known as the orientalis coloration type of the medicinal leech Hirudo medicinalis. In this paper, the new species is formally described as Hirudo orientalis sp. n...
  45. pmc A backseat driver
    I R Schwab
    University of California, Davis, Sacramento, California, USA
    Br J Ophthalmol 90:1447. 2006
  46. ncbi Proteins and peptides of the salivary gland secretion of medicinal leeches Hirudo verbana, H. medicinalis, and H. orientalis
    I P Baskova
    Biological Faculty, Lomonosov Moscow State University, Moscow, Russia
    Biochemistry (Mosc) 73:315-20. 2008
    ..This opens prospects for using certain species of medicinal leech for targeted therapy of various pathologies...
  47. ncbi Genetic differentiation between two species of the medicinal leech, Hirudo medicinalis and the neglected H. verbana, based on random-amplified polymorphic DNA
    Peter Trontelj
    Biotechnical Faculty, Department of Biology, University of Ljubljana, 1000 Ljubljana, Slovenia
    Parasitol Res 94:118-24. 2004
    ..The prevailing view is that all varieties of medicinal leech in Europe represent the same species, Hirudo medicinalis. However, the present study based on RAPD molecular markers demonstrates that a second European taxon, H...
  48. ncbi Sodium pumps adapt spike bursting to stimulus statistics
    Sara Arganda
    Neural Processing Laboratory, Instituto Nicolás Cabrera de Física de Materiales, Facultad de Ciencias, C XVI, Universidad Autonoma de Madrid, Spain
    Nat Neurosci 10:1467-73. 2007
    ..We took intracellular recordings from a spike-bursting sensory neuron in the leech Hirudo medicinalis in response to naturalistic tactile stimuli with different statistical distributions...
  49. ncbi Modulation of swimming behavior in the medicinal leech. IV. Serotonin-induced alteration of synaptic interactions between neurons of the swim circuit
    P S Mangan
    Department of Biology, University of Virginia, Charlottesville 22901
    J Comp Physiol A 175:723-36. 1994
    Serotonin enhances the expression of swimming in the medicinal leech Hirudo medicinalis. These two reports examine the physiological causes underlying this modulation. The initial paper (Mangan et al...
  50. ncbi Seasonal variation of serotonin content and nonassociative learning of swim induction in the leech Hirudo medicinalis
    S Catarsi
    Department of Physiology and Biochemistry, University of Pisa, Italy
    J Comp Physiol A 167:469-74. 1990
    ..This finding strengthens the hypothesis of serotonin as the neurotransmitter mediating dishabituation in swim induction of the leech...
  51. ncbi Recovery after anaerobic metabolism in the leech (Hirudo medicinalis L.)
    H Schmidt
    , , Germany
    J Comp Physiol B 163:574-80. 1993
    Medicinal leeches (Hirudo medicinalis L...
  52. ncbi The whole-body shortening reflex of the medicinal leech: motor pattern, sensory basis, and interneuronal pathways
    B K Shaw
    Department of Biology 0357, University of California, San Diego, La Jolla 92093 0357, USA
    J Comp Physiol A 177:667-81. 1995
    ..wall, and isolated nerve cord - to begin to elucidate the neural basis of this reflex in the medicinal leech Hirudo medicinalis. The motor pattern of the reflex involved an activation of excitatory motor neurons innervating dorsal and ..
  53. ncbi Protease inhibitors in the alimentary tract of the medicinal leech Hirudo medicinalis: in vivo and in vitro studies
    F J Roters
    , , , FRG
    J Comp Physiol B 162:85-92. 1992
    ..A hypothetical role of the inhibitors in the preservation of the blood stored in the foregut is discussed...
  54. ncbi Functionally heterogeneous segmental oscillators generate swimming in the medical leech
    C G Hocker
    Department of Biology, NSF Center for Biological Timing, University of Virginia, Charlottesville, VA 22903 2477, USA
    J Comp Physiol A 186:871-83. 2000
    Swimming behavior in the leech Hirudo medicinalis arises from neuronal circuits within the ventral nerve cord...
  55. ncbi Ion transport across leech integument. I. Electrogenic Na+ transport and current fluctuation analysis of the apical Na+ channel
    W M Weber
    , , Germany
    J Comp Physiol B 163:153-9. 1993
    The dorsal skin of the leech Hirudo medicinalis was used for electrophysiological measurements performed in Ussing chambers. The leech skin is a tight epithelium (transepithelial resistance = 10.5 +/- 0.5 k omega...
  56. ncbi Assessment of genotoxic potency of sulfate-rich surface waters on medicinal leech and human leukocytes using different versions of the Comet assay
    Zlatko Mihaljević
    Department of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia
    Ecotoxicol Environ Saf 74:1416-26. 2011
    ..how exposure to sulfate-rich surface waters affects the level of primary DNA damage in hemocytes of leech Hirudo medicinalis. Samples of surface water were collected at two sites near a gypsum factory (Knin, Croatia) and two ..
  57. ncbi Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-c-subtilisin Carlsberg and CI-2-subtilisin Novo
    C A McPhalen
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Biochemistry 27:6582-98. 1988
    ..inhibitors have been determined: subtilisin Carlsberg with the recombinant form of eglin-c from the leech Hirudo medicinalis and subtilisin Novo with chymotrypsin inhibitor 2 from barley seeds...
  58. pmc Acid/base transport across the leech giant glial cell membrane at low external bicarbonate concentration
    J W Deitmer
    Abteilung fur Allgemeine Zoologie, FB Biologie, Universitat Kaiserslautern, Postfach 3049, D 67653 Kaiserslautern, Germany
    J Physiol 512:459-69. 1998
    ..We have studied acid/base transport across the cell membrane of the giant neuropile glial cell in the leech (Hirudo medicinalis) central nervous system induced by changing the external pH (pHo), using double-barrelled, pH-sensitive ..
  59. ncbi Putative nitric oxide synthase (NOS)-containing cells in the central nervous system of the leech, Hirudo medicinalis: NADPH-diaphorase histochemistry
    L D Leake
    School of Biological Sciences, University of Portsmouth, UK
    Brain Res 723:115-24. 1996
    ..synthase (NOS)-containing cells in whole-mount preparations of the central nervous system of the leech, Hirudo medicinalis, were studied using NADPH-diaphorase (NADPH-d) histochemistry...
  60. ncbi Structure of the complex of the antistasin-type inhibitor bdellastasin with trypsin and modelling of the bdellastasin-microplasmin system
    U Rester
    Abteilung Strukturforschung, Max Planck Institut fur Biochemie, Am Klopferspitz 18a, Martinsried, 82152, Germany
    J Mol Biol 293:93-106. 1999
    ..The leech-derived (Hirudo medicinalis) antistasin-type inhibitor bdellastasin represents a specific inhibitor of trypsin and plasmin and thus ..
  61. ncbi Effects of extracellular purines on ion transport across the integument of Hirudo medicinalis
    Mikael Schnizler
    Institut für Tierphysiologie der Justus Liebig Universität Giessen, Wartweg 95, D 35392 Giessen Germany
    J Exp Biol 205:2705-13. 2002
    ..the short-term regulation of transepithelial ion transport across the dorsal integument of the leech Hirudo medicinalis, and we have established a model of Na+ uptake...
  62. ncbi Historical Article: Hirudo medicinalis: ancient origins of, and trends in the use of medicinal leeches throughout history
    I S Whitaker
    Department of Anatomy, University of Cambridge, Cambridge, UK
    Br J Oral Maxillofac Surg 42:133-7. 2004
    Blood letting and the therapeutic use of Hirudo medicinalis date back to ancient Egypt and the beginning of civilisation...
  63. ncbi [The effects of axotomy on the nociceptive neurons of the leech]
    J Pastor-Gómez
    Servicion de Neurofisiología Hospital Universitario La Princesa, Madrid, Espana
    Rev Neurol 35:1001-9. 2002
    ..Lateral N neuron, in the segmental ganglion of the leech Hirudo medicinalis, behave like polimodal nociceptors in mammals, meanwhile medial N cells is similar to mechano nociceptors (..
  64. ncbi Chromosome numbers for three species of medicinal leeches (Hirudo spp.)
    Serge Utevsky
    V N Karazin Kharkiv National University, Kharkiv, Ukraine
    Syst Parasitol 74:95-102. 2009
    Karyological preparations were made from the testisacs of three medicinal leech species Hirudo medicinalis Linnaeus, 1758, H. verbana Carena, 1820 and H. orientalis Utevsky & Trontelj, 2005 ...
  65. ncbi Intracellular Ca2+ release mediated by metabotropic glutamate receptor activation in the leech giant glial cell
    C Lohr
    Abteilung fur Allgemeine Zoologie, Universitat Kaiserslautern, Germany
    J Exp Biol 200:2565-73. 1997
    ..potential (Em) of single, identified neuropile glial cells in the central nervous system of the leech Hirudo medicinalis. Exposed glial cells of isolated ganglia were filled iontophoretically with the Ca2+ indicator dye Fura-2...
  66. pmc Intracellular Ca2+ regulation by the leech giant glial cell
    W Nett
    Abteilung fur Allgemeine Zoologie, FB Biologie, Universitat Kaiserslautern, Postfach 3049, D 67653 Kaiserslautern, Germany
    J Physiol 507:147-62. 1998
    ..fura-2 (for Ca2+) and SBFI (for Na+) in situ in giant glial cells of the central nervous system of the leech Hirudo medicinalis. 2...
  67. pmc Poly-paraphyly of Hirudinidae: many lineages of medicinal leeches
    Anna J Phillips
    Department of Biology, The Graduate Center, The City University of New York, New York, NY, USA
    BMC Evol Biol 9:246. 2009
    ..Notorious throughout the world, the quintessential leech family Hirudinidae has been taken for granted to be monophyletic, as has the non-bloodfeeding family Haemopidae...
  68. ncbi Annelid epithelia as models for electrogenic Na+ transport
    Mikael Schnizler
    Institut für Tierphysiologie der Justus Liebig Universität Giessen, Wartweg 95, Giessen, Germany
    Biochim Biophys Acta 1566:84-91. 2002
    ..We use dissected segments of integuments from the limnic leech Hirudo medicinalis and, recently, from the earthworm Lumbricus terrestris for Ussing chamber experiments...
  69. ncbi Regulation of pharyngeal motility by FMRFamide and related peptides in the medicinal leech, Hirudo medicinalis
    B A O'Gara
    Department of Biological Sciences, Barnard College of Columbia University, 3009 Broadway, New York, NY 10027 6598, USA
    Invert Neurosci 4:41-53. 1999
    ..1 microM) or the cation channel blocker amiloride (100 microM)...
  70. ncbi The phosphoinositide signaling cascade is involved in photoreception in the leech Hirudo medicinalis
    K Ukhanov
    , Tierphysiologie, , Germany
    J Comp Physiol A 186:1171-83. 2000
    ..effects of BAPTA, heparin, and neomycin on electrical light responses were studied in the photoreceptors of Hirudo medicinalis. Light activation produces a fast increase in intracellular Ca2+ concentration (Cai) as detected with the ..
  71. ncbi Calcium transients in subcompartments of the leech Retzius neuron as induced by single action potentials
    A Beck
    Abteilung fur Allgemeine Zoologie, FB Biologie, Universitat Kaiserslautern, Postfach 3049, D 67653 Kaiserslautern, Germany
    J Neurobiol 48:1-18. 2001
    ..induced by a single action potential (AP) in Retzius neurons in situ of isolated ganglia of the leech Hirudo medicinalis using confocal laser scanning microscopy in the cell body, in different axonal branches, and in dendrites...
  72. ncbi Studies on the host specificity of the medicinal blood leech Hirudo medicinalis L
    A Keim
    Institute for Zoology, Department of Parasitology, University of Hohenheim, Germany
    Parasitol Res 79:251-5. 1993
    ..Mammalian blood in the stomach of the medicinal blood leech Hirudo medicinalis gave satisfactory results. In the case of mixed blood samples from H...
  73. ncbi Leech therapy for patients with surgically unsalvageable venous obstruction after revascularized free tissue transfer
    Douglas B Chepeha
    Department of Otolaryngology, University of Michigan Health System, Ann Arbor 48109 0312, USA
    Arch Otolaryngol Head Neck Surg 128:960-5. 2002
    ..To assess the efficacy and associated complications of a leech therapy protocol used for patients with a head and neck free tissue transfer in whom flap viability is threatened because of surgically unsalvageable venous obstruction...
  74. pmc Generalization of habituation and intrinsic sensitization in the leech
    B D Burrell
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Learn Mem 5:405-19. 1998
    Using the shortening reflex of the medicinal leech Hirudo medicinalis we examined stimulus generalization of habituation learning...
  75. ncbi The medicinal leech in Jewish writings
    F Rosner
    Department of Medicine, Mount Sinai Services at Queens Hospital Center, Jamaica, NY, USA
    Isr Med Assoc J 1:296-8. 1999
    ..Modern plastic and microsurgery have rediscovered the medicinal leech, Hirudo medicinalis, to reduce venous congestion...
  76. ncbi Oxytocin-like peptide: a novel epitope colocalized with the FMRFamide-like peptide in the supernumerary neurons of the sex segmental ganglia of leeches--morphological and biochemical characterization; putative anti-diuretic function
    M Salzet
    , ERS CNRS 20, , Villeneuve d Ascq, France
    Brain Res 601:173-84. 1993
    ..ganglia (SG5, SG6) of three species of leeches belonging to different orders: Theromyzon tessulatum, Hirudo medicinalis and Erpobdella octoculata...
  77. ncbi Parallel pathways coordinate crawling in the medicinal leech, Hirudo medicinalis
    A P Baader
    Department of Biology, University of California San Diego, La Jolla 92093 0357, USA
    J Comp Physiol A 176:715-26. 1995
    ....
  78. ncbi The failing flap in facial plastic and reconstructive surgery: role of the medicinal leech
    D S Utley
    Division of Otolaryngology Head and Neck Surgery, Facial Plastic and Reconstructive Surgery, Stanford University Medical Center, Palo Alto Veterans Administration Health Care System, California 94305 5328, USA
    Laryngoscope 108:1129-35. 1998
    To review the use of the medicinal leech, Hirudo medicinalis, in salvaging the failing, venous-congested flap. A protocol for the use of leeches is presented...
  79. ncbi Neurotoxic effects of caulerpenyne
    M Brunelli
    Dipartimento di Fisiologia e Biochimica G Moruzzi, Universita di Pisa, Italy
    Prog Neuropsychopharmacol Biol Psychiatry 24:939-54. 2000
    ..sesquiterpene synthesized by the green alga Caulerpa taxifolia onto the central nervous system of the leech Hirudo medicinalis. Investigations have been performed with three different approaches: neuroethological, electrophysiological ..
  80. ncbi Structural analysis of leech galactocerebrosides using 1D and 2D NMR spectroscopy, gas chromatography-mass spectrometry, and FAB mass spectrometry
    B Zipser
    Department of Physiology, Michigan State University, East Lansing 48824, USA
    Carbohydr Res 308:47-55. 1998
    Cerebrosides were isolated from the leech species, Hirudo medicinalis, and purified to homogeneity by silicic acid chromatography, followed by preparative thin-layer chromatography...
  81. pmc Diverse molecular data demonstrate that commercially available medicinal leeches are not Hirudo medicinalis
    Mark E Siddall
    Division of Invertebrate Zoology, American Museum of Natural History, New York, NY 10024, USA
    Proc Biol Sci 274:1481-7. 2007
    ..inhibitors purified from leech saliva, and notwithstanding the historical association with quackery, Hirudo medicinalis has been approved by the United States Food and Drug Administration as a prescription medical device...
  82. ncbi Probing intermolecular backbone H-bonding in serine proteinase-protein inhibitor complexes
    W Lu
    Gryphon Sciences, 250 East Grand Avenue, Suite 90, South San Francisco, CA 94080, USA
    Chem Biol 6:419-27. 1999
    ..groups of most residues in the binding loop of eglin c, a potent serine proteinase inhibitor from the leech Hirudo medicinalis, are H-bonded to the carbonyl groups of residues in the target enzyme molecules such as chymotrypsin, ..
  83. ncbi Hirudin as alternative anticoagulant--a historical review
    Fritz Markwardt
    Pharmacology, International Institute of Thrombosis and Vascular Diseases, Frankfurt M, Germany
    Semin Thromb Hemost 28:405-14. 2002
    ..The most potent known natural thrombin inhibitor from blood-sucking leeches ( Hirudo medicinalis), hirudin has served as a standard for designing the natural coagulation inhibitors as an anticoagulant drug...
  84. ncbi Modulation of swimming behavior in the medicinal leech. III. Control of cellular properties in motor neurons by serotonin
    P S Mangan
    Department of Biology, University of Virginia, Charlottesville 22901
    J Comp Physiol A 175:709-22. 1994
    Expression of swimming in the medicinal leech (Hirudo medicinalis) is modulated by serotonin, a naturally occurring neurohormone. Exogenous application of serotonin engenders 'spontaneous' swimming activity in nerve-cord preparations...
  85. ncbi Characterization of a voltage-dependent conductance in the basolateral membrane of leech skin epithelium
    M Schnizler
    , , Germany
    J Comp Physiol B 168:295-302. 1998
    Voltage clamp studies were performed on the dorsal integument of Hirudo medicinalis. Under apical calcium-free conditions an inward-directed component of transepithelial current was activated by changes of transepithelial voltage...
  86. ncbi Functional mapping of cannabinoid receptor homologs in mammals, other vertebrates, and invertebrates
    John M McPartland
    GW Pharmaceuticals, Porton Down Science Park, Salisbury, Wiltshire SP4 0JQ, UK
    Gene 312:297-303. 2003
    ..Mapping the contested leech (Hirudo medicinalis) CBR sequence suggests that it encodes a functional CB1; it expresses fewer substitutions than the sea ..
  87. ncbi A systematic overview of the medicinal importance of sanguivorous leeches
    S M Abbas Zaidi
    H S Z H Govt Unani Medical College, Behind MANIT, Nehru Nagar Kolar Bypass Road, Bhopal M P, India
    Altern Med Rev 16:59-65. 2011
    ..Because of their important salivary components, blood-sucking (sanguivorous) leeches, such as Hirudo medicinalis and related species, have engendered great interest from pharmaceutical companies searching for ..
  88. ncbi Serotonin induces four pharmacologically separable contractile responses in the pharynx of the leech Hirudo medicinalis
    B A O'Gara
    Department of Biological Sciences, Barnard College of Columbia University, New York, NY 10027 6598, USA
    Gen Pharmacol 32:669-81. 1999
    ..The results of this study suggest that the leech pharynx possesses more than one type of serotonin receptor...
  89. ncbi Delayed leech-borne infection with Aeromonas hydrophilia in escharotic flap wound
    B Ardehali
    Department of Plastic and Reconstructive Surgery, Charing Cross Hospital, Fulham Palace Road, Hammersmith, London W6 8AF, UK
    J Plast Reconstr Aesthet Surg 59:94-5. 2006
    Medicinal leeches (Hirudo medicinalis) are commonly used in plastic surgery for the salvage of congested flaps and replanted parts compromised by venous congestion...
  90. pmc Symbiosis of Aeromonas veronii biovar sobria and Hirudo medicinalis, the medicinal leech: a novel model for digestive tract associations
    J Graf
    Institute for Medical Microbiology, University of Berne, Switzerland
    Infect Immun 67:1-7. 1999
    b>Hirudo medicinalis, the medicinal leech, is applied postoperatively in modern medicine. Infections by Aeromonas occur in up to 20% of patients unless a preemptive antibiotic treatment is administered...
  91. ncbi Kainate responses of leech Retzius neurons in situ and in vitro
    S Lohrke
    Abteilung fur Allgemeine Zoologie, Universitat Kaiserslautern, Germany
    J Neurobiol 31:345-58. 1996
    ..RCs) of intact segmental ganglia (in situ), acutely isolated RCs, and cultured RCs (in vitro) of the leech Hirudo medicinalis. RCs in intact ganglia responded to kainate (5-20 microM) with depolarizations up to 30 mV or with an inward ..
  92. ncbi Leech therapy for symptomatic relief of cancer pain
    Mehmet Emin Kalender
    Department of Medical Oncology, Gaziantep University, Gaziantep, Turkey
    Pain Med 11:443-5. 2010
    Most patients with advanced stage cancer report moderate to severe pain. The leech (Hirudo medicinalis) is commonly used in traditional medicine for relief of localized pain.
  93. ncbi Vascular endothelial growth factor is involved in neoangiogenesis in Hirudo medicinalis (Annelida, Hirudinea)
    G Tettamanti
    Department of Structural and Functional Biology, University of Insubria, via J H Dunant 3, 21100 Varese, Italy
    Cytokine 22:168-79. 2003
    ..In this study the role of VEGF in neovessel formation is investigated in Hirudo medicinalis. The leech is able to respond to administration of human VEGF by formation of new vessels. The response of H...
  94. ncbi Infection risk related to the use of medicinal leeches
    Tiene G M Bauters
    Department of Pharmacy, University Hospital, De Pintelaan 185, B 9000, Ghent, Belgium
    Pharm World Sci 29:122-5. 2007
    ..To assess the incidence of postoperative wound infections related to treatment with medicinal leeches at Ghent University Hospital...
  95. ncbi Application of the comet assay and detection of DNA damage in haemocytes of medicinal leech affected by aluminium pollution: a case study
    Zlatko Mihaljević
    Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10 000 Zagreb, Croatia
    Environ Pollut 157:1565-72. 2009
    ..The results indicate the DNA damaging potential of aluminium compounds present in an excess amount in tested samples...
  96. pmc The evolution and comparative neurobiology of endocannabinoid signalling
    Maurice R Elphick
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK
    Philos Trans R Soc Lond B Biol Sci 367:3201-15. 2012
    ..there is evidence that 2-AG mediates retrograde signalling at synapses in the nervous system of the leech Hirudo medicinalis by activating presynaptic transient receptor potential vanilloid-type ion channels...
  97. ncbi Microglia of medicinal leech (Hirudo medicinalis) express a specific activation marker homologous to vertebrate ionized calcium-binding adapter molecule 1 (Iba1/alias aif-1)
    Francesco Drago
    Universite Lille 1, Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée, EA4550, Villeneuve d Ascq, 59655, France
    Dev Neurobiol 74:987-1001. 2014
    ..The medicinal leech Hirudo medicinalis is able to regenerate its CNS after injury, leading to a complete functional repair...
  98. ncbi Use of scanning ion conductance microscopy to guide and redirect neuronal growth cones
    Mario Pellegrino
    Dipartimento di Scienze Fisiologiche, Universita di Pisa, Pisa, Italy
    Neurosci Res 64:290-6. 2009
    ..Thanks to its deterministic and interactive features, this novel approach to guiding growth cones is a promising way to develop networks of identified neurons as well as link them with artificial structures...
  99. ncbi The use of medical leeches for venous congestion. A review and case report
    N J Buote
    Nicole J Buote, DVM DACVS, 1818 South Sepulveda Blvd, Los Angeles, CA 90025, United States, Phone 1 310 473 2951, E mail
    Vet Comp Orthop Traumatol 27:173-8. 2014
    The medicinal leech, Hirudo medicinalis, has been used for hundreds of years in human medicine for a variety of diseases, most recently including venous congestion following reconstructive surgeries (skin flaps and reimplantations), ..
  100. ncbi Gastrointestinal bleed after leeching in a patient on aspirin therapy
    Naveen Kumar
    Department of Medicine, Dayanand Medical College and Hospital, Ludhiana, India
    Indian J Gastroenterol 24:170. 2005
    Leeches ( Hirudo medicinalis ) have been used in health care for their property of bloodletting. Bleed occurring from the site of leech attachment has been well documented...
  101. ncbi Automated querying and identification of novel peptides using MALDI mass spectrometric imaging
    Jocelyne Bruand
    University of California, San Diego, La Jolla, California 92093, United States
    J Proteome Res 10:1915-28. 2011
    ..We tested our approach, MSI-Query, on a number of ROIs in the medicinal leech, Hirudo medicinalis, including the central nervous system (CNS)...

Research Grants6

  1. TRANSPORT AND SORTING OF NEURONAL MEMBRANE PROTEINS
    RICHARD ROTUNDO; Fiscal Year: 1990
    ..to inject synthesized AChE, VSV G-glycoprotein, and IL2 receptor mRNA into identified neurons of the leech (Hirudo medicinalis) to study the transport and localization of an identified rapidly transported protein and foreign membrane ..
  2. NAVIGATION AND GUIDANCE--IDENTIFIED GROWTH CONE ARRAY
    John Jellies; Fiscal Year: 1991
    ..examine growth cone navigation and guidance in a recently described identified cell in the medicinal leech, Hirudo medicinalis. Each of these Comb- or C-cells projects an array of about 70 individual, parallel growth cones along an ..
  3. TRANSPORT AND SORTNG OF NEURONAL MEMBRANE PROTEINS
    RICHARD ROTUNDO; Fiscal Year: 1992
    ..in vitro synthesized AChE, VSV G-glycoprotein, and IL2 receptor mRNA into identified neurons of the leech (Hirudo medicinalis) to study the transport and localization of an identified rapidly transported protein and foreign membrane ..
  4. HOMEBOX GENES AND NEURONAL DIFFERENTIATION
    GABRIEL AISEMBERG; Fiscal Year: 2002
    ..Nine Hox/HOM genes have been cloned and partially characterized in the leech Hirudo medicinalis. These genes are expressed in different but overlapping sets of neurons...
  5. NEURONAL REGULATION OF BEHAVIOR
    WOLFGANG FRIESEN; Fiscal Year: 1992
    ..Experiments will be carried out on the ventral nerve cord and cephalic ganglia of the leech, Hirudo medicinalis. Standard physiological and morphological techniques will be employed to identify neurons, to describe ..
  6. Receptor Phosphatases and Process Outgrowth
    Eduardo Macagno; Fiscal Year: 2005
    ....