scid mice

Summary

Summary: Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.

Top Publications

  1. ncbi NOD/SCID/gamma(c)(null) mouse: an excellent recipient mouse model for engraftment of human cells
    Mamoru Ito
    Central Institute for Experimental Animals, Department of Pediatrics, Graduate School of Medicine, Kyoto University, 1430 Nogawa, Miyamae, Kawasaki, Kanagawa 216 0001, Japan
    Blood 100:3175-82. 2002
  2. pmc ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome
    Christophe Ginestier
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Stem Cell 1:555-67. 2007
  3. pmc Prospective identification of tumorigenic breast cancer cells
    Muhammad Al-Hajj
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 100:3983-8. 2003
  4. ncbi Identification of pancreatic cancer stem cells
    Chenwei Li
    Department of Surgery, University of Michigan Medical Center, 1500 East Medical Center Drive, Ann Arbor, MI 48109, USA
    Cancer Res 67:1030-7. 2007
  5. ncbi Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines
    Jeongwu Lee
    Neuro Oncology Branch, National Cancer Institute, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cancer Cell 9:391-403. 2006
  6. pmc Efficient tumour formation by single human melanoma cells
    Elsa Quintana
    Howard Hughes Medical Institute, Life Sciences Institute, Department of Internal Medicine, and Center for Stem Cell Biology, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Nature 456:593-8. 2008
  7. ncbi Identification of human brain tumour initiating cells
    Sheila K Singh
    The Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children and University of Toronto, 555 University Avenue, Toronto, M5G 1X8, Canada
    Nature 432:396-401. 2004
  8. ncbi Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties
    Dario Ponti
    Department of Experimental Oncology and Laboratories, Istituto Nazionale Tumori, Milan, Italy
    Cancer Res 65:5506-11. 2005
  9. ncbi Humanized mice in translational biomedical research
    Leonard D Shultz
    The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA
    Nat Rev Immunol 7:118-30. 2007
  10. ncbi Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells
    Leonard D Shultz
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    J Immunol 174:6477-89. 2005

Research Grants

  1. Role of NGF in inflammatory and proliferative cascades of psoriatic disease
    Siba P Raychaudhuri; Fiscal Year: 2012
  2. Bandana Chatterjee; Fiscal Year: 2016
  3. RONALD JAY KOENIG; Fiscal Year: 2014
  4. The voltage-gated Kv1.3 channel in psoriatic disease: A novel therapeutic target
    Siba P Raychaudhuri; Fiscal Year: 2012
  5. Amelioration of cisplatin ototoxicity by transplatin
    Debashree Mukherjea; Fiscal Year: 2010
  6. Combination therapies for chronic HBV, liver disease and cancer
    Mark A Feitelson; Fiscal Year: 2012
  7. Combined use of green tea and quercetin in prostate cancer
    SUSANNE MARGARETE HENNING; Fiscal Year: 2012
  8. Growth Factor and Antiapoptotic Gene Delivery to Human Islets
    Ram I Mahato; Fiscal Year: 2012
  9. Experimental engineering of ovarian grafting to promote angiogenesis for fertilit
    GLENN LEWIS SCHATTMAN; Fiscal Year: 2010
  10. Development of transformed lactobacilli as a microbicide
    Richard B Markham; Fiscal Year: 2010

Detail Information

Publications388 found, 100 shown here

  1. ncbi NOD/SCID/gamma(c)(null) mouse: an excellent recipient mouse model for engraftment of human cells
    Mamoru Ito
    Central Institute for Experimental Animals, Department of Pediatrics, Graduate School of Medicine, Kyoto University, 1430 Nogawa, Miyamae, Kawasaki, Kanagawa 216 0001, Japan
    Blood 100:3175-82. 2002
    ..mutation (gamma(c)(null)) were generated by 8 backcross matings of C57BL/6J-gamma(c)(null) mice and NOD/Shi-scid mice. When human CD34+ cells from umbilical cord blood were transplanted into this strain, the engraftment rate in ..
  2. pmc ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome
    Christophe Ginestier
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Stem Cell 1:555-67. 2007
    ..These findings offer an important new tool for the study of normal and malignant breast stem cells and facilitate the clinical application of stem cell concepts...
  3. pmc Prospective identification of tumorigenic breast cancer cells
    Muhammad Al-Hajj
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 100:3983-8. 2003
    ..Furthermore, because these cells drive tumor development, strategies designed to target this population may lead to more effective therapies...
  4. ncbi Identification of pancreatic cancer stem cells
    Chenwei Li
    Department of Surgery, University of Michigan Medical Center, 1500 East Medical Center Drive, Ann Arbor, MI 48109, USA
    Cancer Res 67:1030-7. 2007
    ....
  5. ncbi Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines
    Jeongwu Lee
    Neuro Oncology Branch, National Cancer Institute, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cancer Cell 9:391-403. 2006
    ..These findings suggest that TSCs may be a more reliable model than many commonly utilized cancer cell lines for understanding the biology of primary human tumors...
  6. pmc Efficient tumour formation by single human melanoma cells
    Elsa Quintana
    Howard Hughes Medical Institute, Life Sciences Institute, Department of Internal Medicine, and Center for Stem Cell Biology, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Nature 456:593-8. 2008
    ..However, the extent to which NOD/SCID mice underestimate the frequency of tumorigenic human cancer cells has been uncertain...
  7. ncbi Identification of human brain tumour initiating cells
    Sheila K Singh
    The Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children and University of Toronto, 555 University Avenue, Toronto, M5G 1X8, Canada
    Nature 432:396-401. 2004
    ....
  8. ncbi Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties
    Dario Ponti
    Department of Experimental Oncology and Laboratories, Istituto Nazionale Tumori, Milan, Italy
    Cancer Res 65:5506-11. 2005
    ..marker Oct-4, and gave rise to new tumors when as few as 10(3) cells were injected into the mammary fat pad of SCID mice. Long-term cultures of breast tumorigenic cells with stem/progenitor cell properties represent a suitable in ..
  9. ncbi Humanized mice in translational biomedical research
    Leonard D Shultz
    The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA
    Nat Rev Immunol 7:118-30. 2007
    ....
  10. ncbi Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells
    Leonard D Shultz
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    J Immunol 174:6477-89. 2005
    ..generate 6-fold higher percentages of human CD45(+) cells in host bone marrow than with similarly treated NOD-scid mice. These human cells include B cells, NK cells, myeloid cells, plasmacytoid dendritic cells, and HSC...
  11. pmc Hedgehog signaling and Bmi-1 regulate self-renewal of normal and malignant human mammary stem cells
    Suling Liu
    Comprehensive Cancer Center, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109 0946, USA
    Cancer Res 66:6063-71. 2006
    ..These studies support a cancer stem cell model in which the hedgehog pathway and Bmi-1 play important roles in regulating self-renewal of normal and tumorigenic human mammary stem cells...
  12. ncbi Hepatitis C virus replication in mice with chimeric human livers
    D F Mercer
    Surgical-Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Nat Med 7:927-33. 2001
    ..By transplanting normal human hepatocytes into SCID mice carrying a plasminogen activator transgene (Alb-uPA), we generated mice with chimeric human livers...
  13. ncbi Cancer stem cells--perspectives on current status and future directions: AACR Workshop on cancer stem cells
    Michael F Clarke
    Stanford University School of Medicine, Stanford, California, USA
    Cancer Res 66:9339-44. 2006
  14. pmc Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature
    Emmanuelle Charafe-Jauffret
    Centre de Recherche en Cancerologie de Marseille, Laboratoire d Oncologie Moleculaire, UMR891 Inserm Institut Paoli Calmettes, Universite de la Mediterranee, Marseille, France
    Cancer Res 69:1302-13. 2009
    ....
  15. ncbi Melanoma contains CD133 and ABCG2 positive cells with enhanced tumourigenic potential
    Elena Monzani
    Department of Biomolecular Science and Biotechnology, University of Milan, Milan, Italy
    Eur J Cancer 43:935-46. 2007
    ..a subset of cells expressing CD133 (CD133+) and the latter is able to develop a Mart-1 positive tumour in NOD-SCID mice. Secondly, the WM115, a human melanoma cell line, has been found to express both CD133 and ABCG2 markers...
  16. ncbi Humanized mice mount specific adaptive and innate immune responses to EBV and TSST-1
    Michael W Melkus
    Department of Internal Medicine, Division of Infectious Diseases, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    Nat Med 12:1316-22. 2006
    Here we show that transplantation of autologous human hematopoietic fetal liver CD34+ cells into NOD/SCID mice previously implanted with human fetal thymic and liver tissues results in long-term, systemic human T-cell homeostasis...
  17. ncbi Isolation and characterization of multipotent progenitor cells from the Bowman's capsule of adult human kidneys
    Costanza Sagrinati
    Interdepartmental Laboratory of Cellular and Molecular Nephrology, University of Florence, Viale Pieraccini 6, 50139, Firenze, Italy
    J Am Soc Nephrol 17:2443-56. 2006
    ..The injection of CD24+CD133+ PEC but not of CD24-CD133- renal cells into SCID mice that had acute renal failure resulted in the regeneration of tubular structures of different portions of the ..
  18. ncbi CD133 positive hepatocellular carcinoma cells possess high capacity for tumorigenicity
    Shengyong Yin
    Department of General Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, People s Republic of China
    Int J Cancer 120:1444-50. 2007
    ..The implication that CD133 might be one of the markers for HCC cancer stem-like cells needed further investigation...
  19. ncbi A human colon cancer cell capable of initiating tumour growth in immunodeficient mice
    Catherine A O'Brien
    Division of Cell and Molecular Biology, University Health Network, Toronto, Ontario, M5G 1L7, Canada
    Nature 445:106-10. 2007
    ..Here we use renal capsule transplantation in immunodeficient NOD/SCID mice to identify a human colon cancer-initiating cell (CC-IC)...
  20. pmc Increased vaccine efficacy against tuberculosis of recombinant Mycobacterium bovis bacille Calmette-Guérin mutants that secrete listeriolysin
    Leander Grode
    Max Planck Institute for Infection Biology, Berlin, Germany
    J Clin Invest 115:2472-9. 2005
    ..We concluded that superior vaccine efficacy of DeltaureC hly+ rBCG as compared with parental BCG is primarily based on improved cross-priming, which causes enhanced T cell-mediated immunity...
  21. pmc A novel chemokine receptor for SDF-1 and I-TAC involved in cell survival, cell adhesion, and tumor development
    Jennifer M Burns
    ChemoCentryx, Inc, Mountain View, CA 94043, USA
    J Exp Med 203:2201-13. 2006
    ....
  22. pmc Antiretroviral pre-exposure prophylaxis prevents vaginal transmission of HIV-1 in humanized BLT mice
    Paul W Denton
    Department of Internal Medicine, Division of Infectious Diseases, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, United States of America
    PLoS Med 5:e16. 2008
    ..Given the importance of this route of transmission, we investigated the susceptibility of humanized mice to intravaginal HIV-1 infection...
  23. ncbi Mesenchymal stem cells promote engraftment of human umbilical cord blood-derived CD34(+) cells in NOD/SCID mice
    Willy A Noort
    Department of Hematology, Leiden University Medical Center, Leiden University Medical Center, The Netherlands
    Exp Hematol 30:870-8. 2002
    ..In cotransplantation experiments, we examined the homing of MSC as well as the effect on engraftment of human umbilical cord blood (UCB)-derived CD34(+) cells in NOD/SCID mice.
  24. pmc Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro
    Brett D Lindenbach
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:3805-9. 2006
    ..These results greatly extend the utility of the HCVcc genetic system to allow the complete in vitro and in vivo dissection of the HCV life cycle...
  25. ncbi Identification and characterization of tumorigenic liver cancer stem/progenitor cells
    Stephanie Ma
    Department of Pathology, The University of Hong Kong, Pokfulam, Hong Kong, China
    Gastroenterology 132:2542-56. 2007
    ..The aim of this study is to identify, isolate, and characterize the CSC population that drives and maintains hepatocellular carcinoma (HCC) growth and metastasis...
  26. ncbi Pluripotency of mesenchymal stem cells derived from adult marrow
    Yuehua Jiang
    Stem Cell Institute, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Nature 418:41-9. 2002
    ..As MAPCs proliferate extensively without obvious senescence or loss of differentiation potential, they may be an ideal cell source for therapy of inherited or degenerative diseases...
  27. pmc An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation
    C Asseman
    Nuffield Department of Surgery, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, United Kingdom
    J Exp Med 190:995-1004. 1999
    ..These findings provide the first clear evidence that IL-10 plays a nonredundant role in the functioning of regulatory T cells that control inflammatory responses towards intestinal antigens...
  28. ncbi IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis
    Muneo Numasaki
    Department of Geriatric and Respiratory Medicine, Tohoku University School of Medicine, Sendai, Japan
    J Immunol 175:6177-89. 2005
    ..the in vitro growth, whereas IL-17 transfectants grew more rapidly compared with controls when transplanted in SCID mice. This IL-17-elicited enhancement of NSCLC growth was associated with increased tumor vascularity...
  29. ncbi Reconstitution of a functional human immune system in immunodeficient mice through combined human fetal thymus/liver and CD34+ cell transplantation
    Ping Lan
    Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, 02129, USA
    Blood 108:487-92. 2006
    ..to the development of sustained human hematopoiesis and a functional human immune system in immunodeficient NOD/SCID mice. The humanized mice showed systemic repopulation with a comprehensive array of human lymphohematopoietic cells, ..
  30. pmc Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4
    T Takahashi
    Department of Experimental Pathology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    J Exp Med 192:303-10. 2000
    ..This unique function of CTLA-4 could be exploited to potentiate T cell-mediated immunoregulation, and thereby to induce immunologic tolerance or to control autoimmunity...
  31. doi The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis
    Qihong Huang
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Nat Cell Biol 10:202-10. 2008
    ..Taken together, our findings indicate that miRNAs are involved in tumour migration and invasion, and implicate miR-373 and miR-520c as metastasis-promoting miRNAs...
  32. ncbi Complete reconstitution of human lymphocytes from cord blood CD34+ cells using the NOD/SCID/gammacnull mice model
    Hidefumi Hiramatsu
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, 54 Kawaharacho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Blood 102:873-80. 2003
    ..NOD/SCID/ gammacnull mice provide a unique model to investigate human lymphopoiesis without the cotransplantation of human tissues...
  33. pmc Outer-surface protein C of the Lyme disease spirochete: a protein induced in ticks for infection of mammals
    Dorothee Grimm
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South Fourth Street, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 101:3142-7. 2004
    ..The induction of a spirochetal virulence factor preceding the time and host in which it is required demonstrates a developmental sequence for transmission of this arthropod-borne pathogen...
  34. ncbi Side population cells isolated from mesenchymal neoplasms have tumor initiating potential
    Colleen Wu
    Program in Developmental and Stem Cell Biology, Toronto Medical Discovery Tower, Hospital for Sick Children, Toronto, ON, Canada
    Cancer Res 67:8216-22. 2007
    ..In addition, our data suggest that the proportion of SP cells could be used as a prognostic factor and that therapeutically targeting this subpopulation of cells could be used to improve patient outcome...
  35. pmc Humanized NOD/SCID/IL2Rgamma(null) mice transplanted with hematopoietic stem cells under nonmyeloablative conditions show prolonged life spans and allow detailed analysis of human immunodeficiency virus type 1 pathogenesis
    Satoru Watanabe
    Department of Virology, Division of Medical Science, Medical Research Institute, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    J Virol 81:13259-64. 2007
    ..These capacities of the hNOG mice are very attractive for modeling mechanisms of AIDS progression and therapeutic strategy...
  36. doi Factors determining successful engraftment of hepatocytes and susceptibility to hepatitis B and C virus infection in uPA-SCID mice
    Thomas Vanwolleghem
    Center for Vaccinology, Ghent University and Hospital, Gent, Belgium
    J Hepatol 53:468-76. 2010
    ..The human liver-uPA(+/+)-SCID mouse is currently the best small animal model available for viral hepatitis infection studies...
  37. pmc Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand
    Beate Heissig
    Division of Hematology Oncology, Cornell University Medical College, New York, NY 10021, USA
    Cell 109:625-37. 2002
    ..Release of sKitL by MMP-9 enables BM repopulating cells to translocate to a permissive vascular niche favoring differentiation and reconstitution of the stem/progenitor cell pool...
  38. ncbi Human NK cell infusions prolong survival of metastatic human neuroblastoma-bearing NOD/scid mice
    Roberta Castriconi
    Department of Experimental Medicine, University of Genoa, Genoa, Italy
    Cancer Immunol Immunother 56:1733-42. 2007
    ..However, homing properties, safety and therapeutic efficacy of NK cell infusions need to be evaluated in a suitable preclinical murine NB model...
  39. ncbi Involvement of chemokine receptors in breast cancer metastasis
    A Muller
    Department of Immunology, DNAX Research Institute, Palo Alto, California 94304, USA
    Nature 410:50-6. 2001
    ..Our findings indicate that chemokines and their receptors have a critical role in determining the metastatic destination of tumour cells...
  40. ncbi Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet
    Susetta Finotto
    Critical Care and Pulmonary Division, Division of Gastroenterology, Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Science 295:336-8. 2002
    ..Thus, T-bet deficiency, in the absence of allergen exposure, induces a murine phenotype reminiscent of both acute and chronic human asthma...
  41. ncbi Human plasmacytoid dendritic cells regulate immune responses to Epstein-Barr virus (EBV) infection and delay EBV-related mortality in humanized NOD-SCID mice
    Wai Hon Lim
    Transplantation Immunology Laboratory, Department of Medicine, Queen Elizabeth Hospital, University of Adelaide, South Australia, Australia
    Blood 109:1043-50. 2007
    ..PDCs) in the development of EBV infection and the onset of lymphoproliferative disease (LPD) in humanized NOD-SCID mice and studied the effect of EBV on PDC function...
  42. ncbi Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation
    T Blunt
    Wellcome Cancer Research Campaign Institute, Cambridge University, England
    Cell 80:813-23. 1995
    ..As DNA-PK binds to DNA ends and is activated by these structures, our findings provide novel insights into V(D)J recombination and DNA repair processes...
  43. ncbi The nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse model of childhood acute lymphoblastic leukemia reveals intrinsic differences in biologic characteristics at diagnosis and relapse
    Richard B Lock
    Children s Cancer Institute Australia for Medical Research School of Paediatrics, University of New South Wales, Australia
    Blood 99:4100-8. 2002
    ..and prognostic features of the patients, the rates at which individual leukemia samples engrafted in NOD/SCID mice were analyzed...
  44. ncbi Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4
    A Peled
    Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Science 283:845-8. 1999
    ..Thus, up-regulation of CXCR4 expression may be useful for improving engraftment of repopulating stem cells in clinical transplantation...
  45. pmc Essential protective role attributed to the surface lipoproteins of Borrelia burgdorferi against innate defences
    Qilong Xu
    Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Mol Microbiol 69:15-29. 2008
    ..burgdorferi after inoculation into the skin of SCID mice. Increasing expression of any of the four randomly chosen surface lipoproteins, OspA, OspE, VlsE or DbpA, fully ..
  46. doi Expression of CD133 on leukemia-initiating cells in childhood ALL
    Charlotte V Cox
    Bristol Institute for Transfusion Sciences, Bristol, United Kingdom
    Blood 113:3287-96. 2009
    ..These data suggest that leukemia-initiating cells in childhood B-ALL have a primitive CD133(+)/CD19(-) and CD38(-) phenotype...
  47. ncbi Growth retardation and leaky SCID phenotype of Ku70-deficient mice
    Y Gu
    The Howard Hughes Medical Institute, Children s Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Immunity 7:653-65. 1997
    ..These unanticipated features of the Ku70-deficient phenotype with respect to lymphocyte development and V(D)J recombination may reflect differential functions of the three DNA-PK components...
  48. ncbi Plasma elevation of stromal cell-derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells
    K Hattori
    James Ewing Laboratory of Developmental Hematopoiesis, Sloan-Kettering Institute for Cancer Research, 1275 York Ave, New York, NY 10021, USA
    Blood 97:3354-60. 2001
    ..These studies lay the foundation for using SDF1 to induce mobilization of hematopoietic stem and progenitor cells in in vivo studies. (Blood. 2001;97:3354-3360)..
  49. ncbi Mobilization of endothelial and hematopoietic stem and progenitor cells by adenovector-mediated elevation of serum levels of SDF-1, VEGF, and angiopoietin-1
    M A Moore
    Laboratory of Developmental Hematopoiesis, Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Ann N Y Acad Sci 938:36-45; discussion 45-7. 2001
    ..a replication-incompetent adenovector expressing the SDF-1 gene to elevate serum levels of SDF-1 in Balb/c and SCID mice. Within 3 to 5 days there was a marked leukocytosis, predominantly involving monocytes, and a three-fold ..
  50. ncbi Human CD34(+)CXCR4(-) sorted cells harbor intracellular CXCR4, which can be functionally expressed and provide NOD/SCID repopulation
    Orit Kollet
    Immunology Department, Weizmann Institute of Science, Rehovot, Israel
    Blood 100:2778-86. 2002
    ..R4(+)) and CD34(+)CXCR4(-) (R4(-)) subsets, sorted with neutralizing anti-CXCR4 mAb, engrafted NOD/SCID mice with significantly lower levels of human cells compared with nonsorted and SDF-1-migrated CD34(+) cells...
  51. pmc OspC facilitates Borrelia burgdorferi invasion of Ixodes scapularis salivary glands
    Utpal Pal
    Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Clin Invest 113:220-30. 2004
    ..These studies conclusively demonstrate the importance of OspC in the invasion of tick salivary glands by B. burgdorferi, a critical step in the transmission of spirochetes from the arthropod vector to the mammalian host...
  52. ncbi Biodistribution and persistence of an MVA-vectored candidate HIV vaccine in SIV-infected rhesus macaques and SCID mice
    Tomas Hanke
    MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, The John Radcliffe, Oxford OX3 9DS, UK
    Vaccine 23:1507-14. 2005
    ..In SIV-infected macaques, MVA.HIVA DNA was undetectable by nested PCR 6 weeks after dosing. In SCID mice, the MVA...
  53. pmc Clinically proven markers of metastasis predict metastatic spread of human melanoma cells engrafted in scid mice
    A Thies
    Zentrum für Experimentelle Medizin, Institut für Anatomie II Experimentelle Morphologie, Universitatsklinikum Hamburg Eppendorf, Martinistrasse 52, Hamburg D 20246, Germany
    Br J Cancer 96:609-16. 2007
    ....
  54. ncbi Regulation of CXCR4 expression in human mesenchymal stem cells by cytokine treatment: role in homing efficiency in NOD/SCID mice
    Mingxia Shi
    Institute of Basic Medical Sciences and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China
    Haematologica 92:897-904. 2007
    ..Homing of MSC to bone marrow (BM) post-transplantation can occur, but does so with only poor efficiency. This study was designed to evaluate the role of the SDF-1/CXCR4 axis in the homing of Flk1+ MSC derived from human fetal BM...
  55. doi The analysis of the functions of human B and T cells in humanized NOD/shi-scid/gammac(null) (NOG) mice (hu-HSC NOG mice)
    Yohei Watanabe
    Department of Microbiology and Immunology, Tohoku University Graduate School of Medicine, Aoba ku, Sendai, Japan
    Int Immunol 21:843-58. 2009
    ..We found that multiple mechanisms were involved in the T-cell abnormalities. Collectively, our results demonstrate that further improvements are necessary before humanized mice with a functional human immune system are achieved...
  56. doi Antitumor activity of gemcitabine and oxaliplatin is augmented by thymoquinone in pancreatic cancer
    Sanjeev Banerjee
    Department of Pathology, Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan, USA
    Cancer Res 69:5575-83. 2009
    ....
  57. ncbi Leukemic B cells clonally identical to myeloma plasma cells are myelomagenic in NOD/SCID mice
    Linda M Pilarski
    Department of Oncology, University of Alberta and Cross Cancer Institute, Edmonton, Alberta, Canada
    Exp Hematol 30:221-8. 2002
    ....
  58. pmc Exposure of immunocompetent adult mice to Pneumocystis carinii f. sp. muris by cohousing: growth of P. carinii f. sp. muris and host immune response
    Chun Li An
    Trudeau Institute, Saranac Lake, NY 12983, USA
    Infect Immun 71:2065-70. 2003
    ..To accomplish this, we exposed adult immunocompetent mice to SCID mice infected with P. carinii f. sp. muris by cohousing. We found that P. carinii f. sp...
  59. ncbi Characterization of C4-2 prostate cancer bone metastases and their response to castration
    Jesco Pfitzenmaier
    Department of Urology, University of Washington, Seattle 98195, Washington, USA
    J Bone Miner Res 18:1882-8. 2003
    ..Here we describe characterization of a model consisting of direct injection of C4-2 cells into tibias...
  60. pmc Borrelia burgdorferi changes its surface antigenic expression in response to host immune responses
    Fang Ting Liang
    Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520 8031, USA
    Infect Immun 72:5759-67. 2004
    ..This change in antigenic expression could be induced by passively immunizing infected severe combined immunodeficiency mice with specific Borrelia antisera or OspC antibody and appears to allow B. burgdorferi to resist immune attack...
  61. ncbi Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo
    Min Jeong Seo
    Department of Physiology, College of Medicine, Pusan National University, Pusan 602 739, Republic of Korea
    Biochem Biophys Res Commun 328:258-64. 2005
    ..Mesenchymal stem cells isolated from human adipose tissue are immunocompatible and are easily isolated. Therefore, hADSC may become an alternative source to hepatocyte regeneration or liver cell transplantation...
  62. ncbi Premature induction of hypertrophy during in vitro chondrogenesis of human mesenchymal stem cells correlates with calcification and vascular invasion after ectopic transplantation in SCID mice
    Karoliina Pelttari
    Orthopaedic University Hospital of Heidelberg, Heidelberg, Germany
    Arthritis Rheum 54:3254-66. 2006
    ....
  63. pmc Systemic correction of storage disease in MPS I NOD/SCID mice using the sleeping beauty transposon system
    Elena L Aronovich
    Department of Genetics, Cell Biology and Development, Center for Genome Engineering, University of Minnesota, Minneapolis, 55455, USA
    Mol Ther 17:1136-44. 2009
    ....
  64. ncbi Positive selection of natural autoreactive B cells
    K Hayakawa
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Science 285:113-6. 1999
    ..Thus, B cells can be subject to positive selection, generated, and maintained on the basis of their autoreactivity...
  65. pmc Revascularization of ischemic limbs after transplantation of human bone marrow cells with high aldehyde dehydrogenase activity
    Benjamin J Capoccia
    Department of Internal Medicine, Washington University School of Medicine, St Louis, MO, USA
    Blood 113:5340-51. 2009
    ..These clinically relevant cells may prove useful in the treatment of critical ischemia in humans...
  66. pmc No requirement for V(D)J recombination in p53-deficient thymic lymphoma
    M J Liao
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill Medical School, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 18:3495-501. 1998
    ....
  67. ncbi Current understanding of stem cell mobilization: the roles of chemokines, proteolytic enzymes, adhesion molecules, cytokines, and stromal cells
    Tsvee Lapidot
    Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel
    Exp Hematol 30:973-81. 2002
    ..Furthermore, the central role of this chemokine in stem cell homing to the bone marrow as well as retention of undifferentiated cells within this tissue will also be discussed...
  68. ncbi SCID-repopulating cell activity of human cord blood-derived CD34- cells assured by intra-bone marrow injection
    Jianfeng Wang
    Department of Hygiene, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyoku, Kyoto, Japan
    Blood 101:2924-31. 2003
    ..the long-term human cell repopulating capacity of our identified CD34(-) SRCs in nonobese diabetic (NOD)/SCID mice. The unveiling of this novel class of primitive human CD34(-) SRCs by IBMI will provide a new concept of the ..
  69. ncbi An orthotopic metastatic prostate cancer model in SCID mice via grafting of a transplantable human prostate tumor line
    Yuwei Wang
    Department of Cancer Endocrinology, BC Cancer Agency, Vancouver, British Columbia, Canada
    Lab Invest 85:1392-404. 2005
    ..Pieces of prostate cancer tissue from a patient were grafted in testosterone-supplemented male NOD-SCID mice at the subrenal capsule graft site permitting high tumor take rates...
  70. pmc Parameters for establishing humanized mouse models to study human immunity: analysis of human hematopoietic stem cell engraftment in three immunodeficient strains of mice bearing the IL2rgamma(null) mutation
    Michael A Brehm
    Diabetes Division, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Clin Immunol 135:84-98. 2010
    ..Our data define key parameters for establishing humanized mouse models to study human immunity...
  71. ncbi Effects of the epidermal growth factor receptor inhibitor OSI-774, Tarceva, on downstream signaling pathways and apoptosis in human pancreatic adenocarcinoma
    Sylvia S W Ng
    Division of Experimental Therapeutics, Ontario Cancer Institute, Princess Margaret Hospital, University of Toronto, Toronto, Ontario M5G 2M9, Canada
    Mol Cancer Ther 1:777-83. 2002
    ..Taken together, these findings support the use of OSI-774 plus a phosphatidylinositol 3'-kinase inhibitor in combination with gemcitabine in the treatment of pancreatic cancer...
  72. ncbi LuCaP 35: a new model of prostate cancer progression to androgen independence
    Eva Corey
    Department of Urology, University of Washington, Seattle, Washington 98195, USA
    Prostate 55:239-46. 2003
    ..Generation of suitable in vivo models is critical for understanding of processes associated with development and progression of prostate cancer (CaP)...
  73. ncbi Soluble receptor activator of nuclear factor kappaB Fc diminishes prostate cancer progression in bone
    Jian Zhang
    Department of Pathology, School of Medicine, Comprehensive Cancer Center, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, MI 48109 0940, USA
    Cancer Res 63:7883-90. 2003
    ..In contrast, sRANK-Fc had no effect on s.c. implanted LuCaP cells. We conclude that sRANK-Fc is an effective inhibitor of RANKL that diminishes progression of CaP growth in bone through inhibition of bone remodeling...
  74. ncbi Cutting edge: in vivo trogocytosis as a mechanism of double negative regulatory T cell-mediated antigen-specific suppression
    Megan S Ford McIntyre
    Multi Organ Transplantation Program, Toronto General Research Institute, University Health Network, University of Toronto, Toronto, Canada
    J Immunol 181:2271-5. 2008
    ..These data provide new insight into how Tregs mediate Ag-specific T cell suppression and may enhance our ability to use DN Tregs as a therapy for transplant rejection and autoimmune diseases...
  75. doi Stable gene transfer and expression in cord blood-derived CD34+ hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system
    Xingkui Xue
    Division of Pediatric Blood and Marrow Transplantation, Berlin, Germany
    Blood 114:1319-30. 2009
    ..Our results support the continuing development of SB-based gene transfer into human hematopoietic stem cells as a modality for gene therapy...
  76. pmc Immortalization eliminates a roadblock during cellular reprogramming into iPS cells
    Jochen Utikal
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Nature 460:1145-8. 2009
    ..Our results show that the acquisition of immortality is a crucial and rate-limiting step towards the establishment of a pluripotent state in somatic cells and underscore the similarities between induced pluripotency and tumorigenesis...
  77. ncbi The essential roles of the chemokine SDF-1 and its receptor CXCR4 in human stem cell homing and repopulation of transplanted immune-deficient NOD/SCID and NOD/SCID/B2m(null) mice
    T Lapidot
    Dept of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Leukemia 16:1992-2003. 2002
    ..In particular, the essential roles of the chemokine SDF-1 and its receptor CXCR4 which mediate and regulate stem cell homing and repopulation will be discussed...
  78. ncbi Tumoricidal activity of tumor necrosis factor-related apoptosis-inducing ligand in vivo
    H Walczak
    Immunex Corporation, Seattle, Washington 98101, USA
    Nat Med 5:157-63. 1999
    ..17 (SCID) mice, and histologic examination of tumors from SCID mice treated with LZ-huTRAIL demonstrated clear areas of apoptotic necrosis within 9-12 hours of injection.
  79. pmc Circulating fibrocytes traffic to the lungs in response to CXCL12 and mediate fibrosis
    Roderick J Phillips
    Division of Pulmonary and Critical Care Medicine, David Geffen School of Medicine at University of California, Los Angeles, USA
    J Clin Invest 114:438-46. 2004
    ..Thus, our results demonstrate, we believe for the first time, that circulating fibrocytes contribute to the pathogenesis of pulmonary fibrosis...
  80. pmc T cell-specific siRNA delivery suppresses HIV-1 infection in humanized mice
    Priti Kumar
    Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Cell 134:577-86. 2008
    ..Moreover, scFvCD7-9R could deliver antiviral siRNAs to naive T cells in Hu-HSC mice and effectively suppress viremia in infected mice. Thus, siRNA therapy for HIV infection appears to be feasible in a preclinical animal model...
  81. pmc Albumin-conjugated C34 peptide HIV-1 fusion inhibitor: equipotent to C34 and T-20 in vitro with sustained activity in SCID-hu Thy/Liv mice
    Cheryl A Stoddart
    Division of Experimental Medicine, Department of Medicine, San Francisco General Hospital, University of California, San Francisco, San Francisco, California 94110, USA
    J Biol Chem 283:34045-52. 2008
    ....
  82. pmc A robust xenotransplantation model for acute myeloid leukemia
    P V Sanchez
    Department of Medicine, Division of Hematology and Oncology, University of Pennsylvania, Philadelphia, PA, USA
    Leukemia 23:2109-17. 2009
    ..We conclude that the NSG xenotransplantation model is a robust model for human AML cell engraftment, which will allow better characterization of AML biology and testing of new therapies...
  83. ncbi High levels of human peripheral blood mononuclear cell engraftment and enhanced susceptibility to human immunodeficiency virus type 1 infection in NOD/LtSz-scid/scid mice
    R M Hesselton
    Department of Pediatrics, University of Massachusetts Medical School, Worcester, USA
    J Infect Dis 172:974-82. 1995
    Inbred C.B-17-scid/scid mice accept human peripheral blood mononuclear cell (PBMC) xenografts and are susceptible to human immunodeficiency virus type 1 (HIV-1) infection, but low levels of PBMC engraftment impede use of this system in ..
  84. ncbi Image-guided radiovirotherapy for multiple myeloma using a recombinant measles virus expressing the thyroidal sodium iodide symporter
    David Dingli
    Molecular Medicine Program, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Blood 103:1641-6. 2004
    ..Testing in other radiosensitive cancers is warranted...
  85. pmc Identification of cells initiating human melanomas
    Tobias Schatton
    Transplantation Research Center, Children s Hospital Boston and Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 451:345-9. 2008
    ..Identification of tumour-initiating cells with enhanced abundance in more advanced disease but susceptibility to specific targeting through a defining chemoresistance determinant has important implications for cancer therapy...
  86. ncbi Expression of hyaluronate and hyaluronate synthase in human primary tumours and their metastases in scid mice
    Milan Jojovic
    Institute for Anatomy, University Hospital Hamburg Eppendorf, Martinistrasse 52, D 20246, Hamburg, Germany
    Cancer Lett 188:181-9. 2002
    ..in metastatic deposits of human breast, colon, ovarian and small-cell lung cancer cell lines transplanted into scid mice using biotinylated hyaluronectin and immunohistochemical staining of hyaluronate synthase...
  87. ncbi NOD/SCID mice engineered to express human IL-3, GM-CSF and Steel factor constitutively mobilize engrafted human progenitors and compromise human stem cell regeneration
    F E Nicolini
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada
    Leukemia 18:341-7. 2004
    ..Our findings suggest that long-term exposure of primitive human hematopoietic cells to elevated levels of human IL-3, GM-CSF and SF in vivo may deleteriously affect the stem cell compartment, while expanding terminal myelopoiesis...
  88. ncbi Selective expansion and engraftment of human CD16+ NK cells in NOD/SCID mice
    Hideki Harada
    Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Kumamoto, Japan
    Eur J Immunol 35:3599-609. 2005
    ..The NOD/SCID mice engrafted with human NK cells exhibited antitumor activity against K562 erythroleukemia in vitro and in vivo...
  89. pmc KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice
    Christopher H Parsons
    Myles H Thaler Center for AIDS and Human Retrovirus Research, Department of Microbiology, University of Virginia Health Systems, Charlottesville, Virginia 22908, USA
    J Clin Invest 116:1963-73. 2006
    ..to evaluate longitudinal patterns of viral gene expression, cell tropism, and immune responses, we injected NOD/SCID mice intravenously with purified virus and measured latent and lytic viral transcripts in distal organs over the ..
  90. ncbi Human cell engraftment after busulfan or irradiation conditioning of NOD/SCID mice
    Elodie Robert-Richard
    INSERM E217, Bordeaux Cedex, France
    Haematologica 91:1384. 2006
    Human hematopoietic stem cell (HSC) xenotransplantation in NOD/SCID mice requires recipient conditioning, classically achieved by sublethal irradiation...
  91. ncbi A new Hu-PBL model for the study of human islet alloreactivity based on NOD-scid mice bearing a targeted mutation in the IL-2 receptor gamma chain gene
    Marie King
    Department of Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01655, USA
    Clin Immunol 126:303-14. 2008
    Immunodeficient NOD-scid mice bearing a targeted mutation in the IL2 receptor common gamma chain (Il2rgamma(null)) readily engraft with human stem cells...
  92. pmc Distinct angiogenic mediators are required for basic fibroblast growth factor- and vascular endothelial growth factor-induced angiogenesis: the role of cytoplasmic tyrosine kinase c-Abl in tumor angiogenesis
    Wei Yan
    Pioneer Valley Life Sciences Institute, Baystate Medical Center University of Massachusetts at Amherst, Springfield, MA 01107, USA
    Mol Biol Cell 19:2278-88. 2008
    ..Furthermore, the wild-type c-Abl enhanced angiogenesis in both Matrigel implantation and tumor xenograft models. These data provide novel insights into c-Abl's differential functions in mediating bFGF- and VEGF-induced angiogenesis...
  93. doi Modification of Borrelia burgdorferi to overproduce OspA or VlsE alters its infectious behaviour
    Qilong Xu
    Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Microbiology 154:3420-9. 2008
    ..alter the ID(50), but affected spirochaetal dissemination and significantly reduced tissue spirochaete loads in SCID mice. In immunocompetent mice, increased vlsE expression resulted in quick clearance of infection, while constitutive ..
  94. doi Human acute leukemia cells injected in NOD/LtSz-scid/IL-2Rgamma null mice generate a faster and more efficient disease compared to other NOD/scid-related strains
    Alice Agliano
    Division of Hematology Oncology, Department of Medicine, European Institute of Oncology, Milan, Italy
    Int J Cancer 123:2222-7. 2008
    ..These models will be instrumental to studies on leukemia-initiating stem cells, leukemia biology, preclinical treatment studies, and to obtain patient-specific preclinical models to design and investigate patient-tailored therapies...
  95. ncbi Transforming growth factor-beta expression and natural killer cell responses during virus infection of normal, nude, and SCID mice
    H C Su
    Division of Biology and Medicine, Brown University, Providence, RI 02912
    J Immunol 151:4874-90. 1993
    ..of NK cell activation in the absence of T cells was evaluated by comparing responses in athymic nude and SCID mice to those in normal C3H/HeN, C57BL/6, and BALB/c mice during lymphocytic choriomeningitis virus (LCMV) infection...
  96. pmc Passive transfer of antibodies protects immunocompetent and imunodeficient mice against lethal Ebola virus infection without complete inhibition of viral replication
    M Gupta
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 75:4649-54. 2001
    ..Transfer of immune serum to SCID mice resulted in 100% survival after lethal challenge with Ebola virus, indicating that antibodies alone can protect ..
  97. ncbi SU11248 is a novel FLT3 tyrosine kinase inhibitor with potent activity in vitro and in vivo
    Anne Marie O'Farrell
    Preclinical Research and Exploratory Development, SUGEN, South San Francisco, CA 94080, USA
    Blood 101:3597-605. 2003
    ..These results suggest that further exploration of SU11248 activity in AML patients is warranted...
  98. ncbi The effects of RANK blockade and osteoclast depletion in a model of pure osteoblastic prostate cancer metastasis in bone
    Peter G Whang
    Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Center for Health Sciences 76 134, 10833 LeConte Avenue, Los Angeles, CA 90095, USA
    J Orthop Res 23:1475-83. 2005
    ..These results indicate that in vivo RANK blockade may not be effective for the prevention of osteoblastic metastasis but may potentially represent a novel therapy that limits the growth of established metastatic CaP lesions in bone...
  99. ncbi Polymorphism in Sirpa modulates engraftment of human hematopoietic stem cells
    Katsuto Takenaka
    Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada
    Nat Immunol 8:1313-23. 2007
    ..Thus, we have identified Sirpa polymorphism as a potent genetic determinant of the engraftment of human hematopoietic stem cells...
  100. ncbi Antitumor activity of 3,5,4'-trimethoxystilbene in COLO 205 cells and xenografts in SCID mice
    Min Hsiung Pan
    Department of Seafood Science, National Kaohsiung Marine University, Nan Tzu, Kaohsiung, Taiwan
    Mol Carcinog 47:184-96. 2008
    ..Our study identifies the novel mechanisms of the antitumor effects of MR-3 and indicates that these results may have significant applications for cancer chemotherapy...
  101. doi Bcl-xL antisense oligonucleotide and cisplatin combination therapy extends survival in SCID mice with established mesothelioma xenografts
    James E Littlejohn
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College of Medicine, College Station, TX, USA
    Int J Cancer 123:202-8. 2008
    ..More notably, the combination of Bcl-xL ASO and cisplatin extends survival in an orthotopic tumor xenograft model...

Research Grants78

  1. Role of NGF in inflammatory and proliferative cascades of psoriatic disease
    Siba P Raychaudhuri; Fiscal Year: 2012
    ..The success of this project will help the VA patient population immensely because both psoriasis and arthritis related sickness is a major problem among this group of patients. ..
  2. Bandana Chatterjee; Fiscal Year: 2016
    ..ShRNAs, inducible protein expression, lentivirus infection, cell proliferation, xenograft tumor growth in SCID mice, protein enrichment and mass spectrometry...
  3. RONALD JAY KOENIG; Fiscal Year: 2014
    ..genes that are induced in PPFP thyroid cancers, and these cells form xenograft tumors and lung metastases in NOD-SCID mice. In the first Specific Aim, chromatin immunoprecipitation - deep sequencing (ChIP-Seq) will be used to identify ..
  4. The voltage-gated Kv1.3 channel in psoriatic disease: A novel therapeutic target
    Siba P Raychaudhuri; Fiscal Year: 2012
    ..3+ lymphocytes pre and post treatment in the transplanted skin. The overall goal of this proposal is to determine whether Kv1.3 blockade constitutes a potential new therapeutic approach to the treatment of psoriasis. ..
  5. Amelioration of cisplatin ototoxicity by transplatin
    Debashree Mukherjea; Fiscal Year: 2010
    ..Such a treatment could improve the quality of life of cancer patients who would otherwise face many years of poor communication because of hearing loss caused by cisplatin treatment. ..
  6. Combination therapies for chronic HBV, liver disease and cancer
    Mark A Feitelson; Fiscal Year: 2012
    ..The best combinations will then be evaluated for long term antiviral efficacy in HBV transgenic SCID mice, which stably replicate wild type HBV and develop CLD (aim 3)...
  7. Combined use of green tea and quercetin in prostate cancer
    SUSANNE MARGARETE HENNING; Fiscal Year: 2012
    ..knock out mice with GT alone, Q alone and GT+ Q together using the concentration shown to be most effective for SCID mice. Lack of the tumor suppressor PTEN in the mouse prostate recapitulates many of the pathological features of CaP ..
  8. Growth Factor and Antiapoptotic Gene Delivery to Human Islets
    Ram I Mahato; Fiscal Year: 2012
    ..islets, transduction of islets with Adv-hHGF-hIL-1Ra prior to transplantation under the kidney capsules of NOD-SCID mice reduced blood glucose and increased the level of serum insulin and c-peptide levels upon glucose challenge...
  9. Experimental engineering of ovarian grafting to promote angiogenesis for fertilit
    GLENN LEWIS SCHATTMAN; Fiscal Year: 2010
    ..PDGF, involved in revascularizing mouse ovaries grafted into skeletal muscle and human ovarian xenografts in NOD-scid mice as a translational model...
  10. Development of transformed lactobacilli as a microbicide
    Richard B Markham; Fiscal Year: 2010
    ..gordonii in progesterone-treated Hu-PBL-SCID mice and by L. rhamnosus in non-progesterone-treated humanized bone marrow liver thymus NOD- SCID (BLT) mice...
  11. SECRETION AND BIOSYNTHESIS OF INSULIN
    Michael L McDaniel; Fiscal Year: 2012
    ..inhibitors or Wnt agonists 1 rapamycin prior to transplanting a "marginal dose" of islets into STZ-diabetic SCID mice. Understanding the co-regulation of the GSK-3/TSC/mTOR &Wnt/GSK-3/-catenin pathways will provide new ..
  12. Inhibition of CCR1/CCR5 mediated angiogenesis and joint destruction by EGCG
    Salah Uddin Ahmed; Fiscal Year: 2010
    ..The success of the proposed experiments may lead to a significant advancement in the development of EGCG as a potentially safe and inexpensive treatment option for RA. ..
  13. Repair of Bone Defects with Human Autologous Pluripotent Very Small Embryonic lik
    Russell S Taichman; Fiscal Year: 2013
    ..Importantly, VSELs from adult humans repaired a calvarial defect in SCID mice. The human VSELs formed new bone when applied in the injured area as measured by u-CT scan, and histological ..
  14. Pankaj Gupta; Fiscal Year: 2014
    ....
  15. Bonnie B Blomberg; Fiscal Year: 2014
    ..and thymus-independent antigens) and adoptive transfer of combinations of young and old B/T cells into SCID mice will be done...
  16. Stem Cell Based Therapy for Regenerative Endodontics
    George T J Huang; Fiscal Year: 2013
    ..stem cells and implanted in canals of human tooth fragments can be vascularized in the subcutaneous space of SCID mice. Therefore, we hypothesize that pulp-like tissue can be regenerated in tooth using stem cells aided with a ..
  17. Intracellular immunization strategy in inhibit HCV related liver cancer
    Srikanta Dash; Fiscal Year: 2010
    ..We have shown that HCV replication in the subcutaneous tumors in the SCID mice is inhibited by interferon alpha...
  18. Murine Models for Mantle Cell Lymphoma: The role of tumor initiating cells (TIC)
    Richard J Ford; Fiscal Year: 2010
    ....
  19. Homing Receptor Determinants of HSC
    Irving L Weissman; Fiscal Year: 2013
    ..In SCID mice (and perhaps humans), single or repetitive (timed to match the opening of another 0...
  20. Development of Vaccine Strategies to prevent EBV+ Lymphoma in Patients with HIV
    ROBERT ALAN BAIOCCHI; Fiscal Year: 2010
    ..The project has great potential to make an impact on public health, job creation and economic growth. ..
  21. DEVELOPMENT AND REGENERATION OF SKIN APPENDAGES
    Cheng Ming Chuong; Fiscal Year: 2013
    ..Transplantation of skin from transgenic mice to SCID mice will help us determine the molecular relationships involved in hair follicle-macro-environment interactions...
  22. Dissecting the Role of HIV-1 Tat &Chemokine Axis in Subtype-Specificity of HAD
    Vinayaka R Prasad; Fiscal Year: 2012
    ..The current application provides evidence that such differences can be reproduced in small, laboratory animals and seeks to delineate the viral genes responsible for HAD. ..
  23. AARON DAVID MICKLE; Fiscal Year: 2014
    ..will determine the role of PTHrP-modulation of TRPV1 in sensory afferents on chronic bone pain in vivo using scid mice xenografts of human breast cancer cells, MDA-MB231-BoM-1833, that metastasize to bones when injected ..
  24. Ayman Al-Hendy; Fiscal Year: 2014
    ..Additionally it will enhance our understanding of the role of steroid hormones in pathogenesis of uterine fibroids using unique in vivo model systems. ..
  25. Kaushal Rege; Fiscal Year: 2015
    ..The efficacy and biocompatibility (toxicity) of polymers and combination treatments will be evaluated in vitro and in vivo (in mice). ..
  26. Ann Arvin; Fiscal Year: 2014
    ..abstract_text> ..
  27. Ah Ng Tony Kong; Fiscal Year: 2015
    ..proliferative genes elicited by dietary PEITC on the growth inhibition of LNCaP orthotopic xenograft tumors in SCID mice. (3) To elucidate the in vitro epigenetic mechanisms of regulation of the genes obtained from in vivo Aims 1 and ..
  28. IVETTE SUAREZ; Fiscal Year: 2015
    ....
  29. Comparison of Human Ehrlichiosis Agent Genomes
    Yasuko Rikihisa; Fiscal Year: 2010
    ..of the antibodies;and/or by immunizing immunocompetent animals with recombinant proteins or passively immunizing SCID mice with specific antibodies, and challenging them with the virulent strain...
  30. Mitochondria and Carcinogenesis
    Keshav K Singh; Fiscal Year: 2010
    ..The proposed study will help determine the contribution of mutant mtDNA to the development of human cancer. ..
  31. Arti Shukla; Fiscal Year: 2016
    ..A) Human MM cells stably overexpressing NLRP3 or empty vector will be injected intraperitoneally (IP) into SCID mice. Two weeks after cell injection mice will receive either saline or chemotherapeutic drugs (Doxorubicin, ..
  32. Non-invasive Assessment of Tissue Engineered Human Oral Mucosa
    Mary Ann Mycek; Fiscal Year: 2012
    ..activity of an engineered tissue in real time and (2) a fourth to assess its viability after grafting in situ in SCID mice. As our model system we will use an engineered tissue, Ex Vivo Produced Oral Mucosal Equivalent (EVPOME), a ..
  33. Ah Ng Tony Kong; Fiscal Year: 2015
    ..in the TRAMP mouse PCa model;(ii) Inhibitory effect of ?-TmT on the formation and growth of LNCaP tumors in the SCID mice;(iii) IHC of re-expression of Nrf2 and anti-oxidative stress/antioxidant genes in ?-TmT-treated TRAMP prostate ..
  34. Inhibition of platelet-leukocyte interactions to treat TRALI
    Paul S Frenette; Fiscal Year: 2010
    ..PMNs by transplantation of cord blood-derived hematopoietic stem and progenitor cells in immunodeficient NOD/SCID mice deficient in the IL-2r common gamma chain...
  35. Non-CD4 Host defense against P. carinii Pneumonia
    Jay K Kolls; Fiscal Year: 2013
    ..Adoptive transfer of either B-cells or antibodies induced by the DC vaccine confer protection to scid mice demonstrating the key role of antibodies in this protection Moreover this serum reacts strongly with the ..
  36. Pathogenesis of Lymphangioleiomyomatosis
    Lucia Schuger; Fiscal Year: 2010
    ..identifying LAM cells based on two novel LAM cell markers), lung explant cultures, and LAM xenografts in SCID mice. Here we specifically propose: 1- To determine whether SRFdownregulation will correct the pro-proteolytic ..
  37. Sherie L Morrison; Fiscal Year: 2015
    ..B. Evaluate the in vivo efficacy of anti-CD20-hIFNa against human B cell lymphoma xenografts in SCID mice. C. Measure the activity of anti-CD20-hIFNa against primary human B cell lymphoma specimens in vitro...
  38. TYPE II 3BETA HSD GENE--REGULATION OF DHEAS SYNTHESIS
    Peter Hornsby; Fiscal Year: 2009
    ..model of adrenocortical cell and molecular biology using transplantation of adrenal cells into adrenalectomized scid mice. In this model, human ZF cells replace the function of the animals'own adrenal glands...
  39. Development of Novel AIDS Gene Therapy Strategies in the Macaque SHIV Model
    Hans Peter Kiem; Fiscal Year: 2012
    ..cassettes in lentivirus and foamy virus vectors to determine the safety of these vectors systems first in NOD/SCID mice and then in the nonhuman primate setting...
  40. Oncolytic Viruses with Therapeutic Genes in the Treatment of Breast Cancer
    DANUTA B KOZBOR; Fiscal Year: 2013
    ..tumor- derived factors (TDFs) in 4T1-bearing syngeneic mice and human breast carcinoma MDA-MB-23 xenografts in SCID mice. The Specific Aims are: 1)...
  41. Testing the safety and efficacy of inhibitory RNA mediated purine analog resistan
    Christopher C Porter; Fiscal Year: 2010
    ..primarily by using 1) xenografts of human CD34+ umbilical cord blood cells (UCBC) in immunocompromised NOD/SCID mice and 2) identification of lentiviral integration sites by LAM-PCR and sequencing, as well as functional ..
  42. cPLA2alpha, COX-2 and TGF-beta in Liver Cancer
    Tong Wu; Fiscal Year: 2011
    ..antisense cPLA2? or COX-2 and these cells will be analyzed for proliferation and apoptosis, in vitro and in SCID mice. Transgenic mice with targeted expression of cPLA2? and COX- 2 in the liver will be developed and utilized to ..
  43. REGULATION OF EBV INFECTION IN EPITHELIAL CELLS
    SHANNON CELESTE KENNEY; Fiscal Year: 2013
    ..of EBV-infected cells in vitro, and determine if BARF1 promotes the growth of EBV- positive epithelial tumors in SCID mice. In Aim 4, we will define the in vivo phenotypes of the Na and BARF1 mutant viruses in our new mouse model ..
  44. Bispecific Antibody Engineering for AML RIT
    Oliver W Press; Fiscal Year: 2013
    ..bispecific Ab pretargeting, with and without cytotoxic chemotherapy, in a disseminated model of human AML in SCID mice. We hypothesize that the novel bispecific "pretargeted" RIT strategy defined in this proposal will amplify the ..
  45. The Role of the Stem Cell Niche in ALL
    Laura F Gibson; Fiscal Year: 2013
    ..SUP-B15 cells also reconstituted leukemia in NOD/SCID mice, as well as demonstrating differentiation in vivo...
  46. Molecular Mechanisms of Myelokathexis
    Andrew Aprikyan; Fiscal Year: 2009
    ..We will also test this model in vivo in NOD-SCID mice and will determine the efficacy of identified inhibitors in vivo...
  47. Development of a Novel, Chimeric Chikungunya Vaccine
    Richard Kinney; Fiscal Year: 2009
    ..Vaccine constructs with the best efficacy will be evaluated for safety using neurovirulence and SCID mice testing...
  48. Sleeping Beauty-mediated Gene Therapy of X-linked SCID
    Kendra A Hyland; Fiscal Year: 2010
    ..Beauty-mediated transposition and long term expression of (c gene in hematopoietic stem cells derived of X-SCID mice, as a model for SB-mediated gene therapy of X-SCID...
  49. Targeting multiple skin cancer pathways using citrus auraptene and ATRA
    J Michael Mathis; Fiscal Year: 2012
    ..We will first examine the effects of a wider range of doses of ATRA 1 AUR against tumor volume in SCID mice. We will compare the effects of AUR treatment in the context of pharmacological suppression of Stat3 (ATRA ..
  50. LAWRENCE MICHAEL KAUVAR; Fiscal Year: 2015
    ..will explore utility in a model for HCMV disease in transplant recipients using human thymus/liver implants in SCID mice. The final deliverable from this project will be an IND-ready native human therapeutic antibody to HCMV, which ..
  51. Oliver W Press; Fiscal Year: 2015
    ..We predict synergistic anti-tumor activity with combinations of PRIT and lenalidomide or bortezomib. We anticipate rapid translation of the results of these preclinical experiments into our clinical RIT program for MM. ..
  52. Hai Ning Shi; Fiscal Year: 2014
    ..provide evidence to indicate that a simultaneous helminth infection also enhances intestinal inflammation in SCID mice that are adoptively transferred with ovalbumin (OVA) specific T cells and infected orally with OVA expressing E...
  53. Tong Wu; Fiscal Year: 2016
    ..We have shown that inhibiting COX-2 prevents the growth of cholangiocarcinoma cells in culture and in SCID mice. However, in light of the increased cardiovascular side effect associated with some COX-2 inhibitors, it is ..
  54. Improving Primordial Follicle Survival After Transplantation of Cryopreserved Hum
    Kutluk H Oktay; Fiscal Year: 2012
    ....
  55. Fatih M Uckun; Fiscal Year: 2015
    ..C-61 plus TBI regimens will be more effective than TBI alone in improving the event-free survival outcome of SCID mice challenged with primary B-lineage ALL cells...
  56. Autologous TGF-B-Modified HSC for Repair of Vasodegenerative Diabetic Retinopathy
    STEPHEN BARTELMEZ; Fiscal Year: 2009
    ..And 4) increases the reparative function of human diabetic CD34 EPC in SCID mice following ischemia/ reperfusion injury...
  57. Synthetic cannabinoids as novel therapeutic strategies against non-small cell lun
    Ramesh K Ganju; Fiscal Year: 2012
    ..The proposed research can yield important novel future clinical therapies that will significantly improve lung cancer mortality and promote lung health. ..
  58. Molecular Imaging of Stem Cell Transplantation in the Heart
    Juri G Gelovani; Fiscal Year: 2010
    ..This approach will provide insights into the fate of human stem cell transplantation into the heart. ..
  59. RO1: A novel and targeted approach to inhibit invasion and angiogenesis
    Fazlul H Sarkar; Fiscal Year: 2012
    ..In the third specific aim, we will test the in vivo anti-tumor activity of B-DIM using an orthotopic PC model in SCID mice as well as in transgenic mouse models, and test whether the anti- tumor activity could be correlated with the ..
  60. Telomerase And Homologous Recombination As Targets In Barrett's Adenocarcinoma
    Masood A Shammas; Fiscal Year: 2012
    ..of telomerase cause suppression of cancer cell growth in vitro and in xenograft model of human BEAC in SCID mice. The mechanisms of telomere maintenance and their activation during carcinogenesis are not known...
  61. Anne E Cress; Fiscal Year: 2015
    ..Metastasis to secondary sites is a cause of skeletal morbidity, disease progression, cancer pain or death. Recurrent disease could be potentially eliminated by adding important "pre-emptive" strategies to conventional therapy. ..
  62. Hybrid Nanoparticles in Imaging and Therapy of Prostate*
    Kattesh Katti; Fiscal Year: 2009
    ..hybrid AuNPs as image enhancers in computed tomographic (CT) and ultrasound (US) imaging of prostate tumors in SCID mice implanted with human prostate cancer xenografts and with experimental modeling, simulations and studies on ..
  63. Xenodiagnosis of Lyme Disease
    Linden T Hu; Fiscal Year: 2012
    ..The repleted nymphal ticks and the SCID mice will be tested for the presence of B. burgdorferi by PCR and culture...
  64. IDENTIFY ANTIGENIC DETERMINANTS OF HUMAN PERIODONTITIS
    YEN TUNG TENG; Fiscal Year: 2001
    ..Engraftment of immunodeficient NOD/SCID mice with human peripheral blood leukocytes (HuPBL) provides an excellent model for studying immune responses to ..
  65. Blood Brain Barrier Physiology and HIV Dementia
    HOWARD GENDELMAN; Fiscal Year: 2007
    ..The data acquired could provide new insights and therapeutic intervention strategies for monocyte BBB trafficking during progressive HIV-1 infection in brain tissue. ..
  66. DEVELOPMENT OF NEW SCID MOUSE MODELS FOR AIDS RESEARCH
    Leonard Shultz; Fiscal Year: 1999
    ..NOD/LtSz-scid/scid mice support heightened levels of human hematopoietic cell reconstitution compared with C.B-17-scid/scid mice...
  67. XENOGENEIC MODEL FOR HOMING OF HUMAN LYMPHOCYTES
    Sharon Evans; Fiscal Year: 1993
    ..by (a) directly comparing human lymphocyte binding in vitro to peripheral lymph node HEV of normal Balb/c and SCID mice and (b) examining the effect of L-selectin-specific blocking antibodies on the adhesion of human lymphocytes to ..
  68. Stampidine: A Novel Broad-Spectrum Antiviral Agent
    Taracad Venkatachalam; Fiscal Year: 2003
    ..dose-dependent in vivo anti-HIV activity against the NRTI-resistant clinical HIV-1 isolate, BR/92/019 in Hu-PBL-SCID mice. Pharmacokinetic and toxicity studies of STAMP conducted in mice, rats, cats, and dogs following oral ..
  69. HGF Induces Robust Human Thymopoiesis in Mice
    Jan Nolta; Fiscal Year: 2004
    ..Even mature human T cells are rapidly cleared following transplantation into NOD/SCID mice. In spite of the drawbacks to the existing systems, the scientific community constantly strives to improve these ..
  70. VIRAL GASTROENTERITIS--BASIS OF PROTECTION AND VIRULENCE
    Harry Greenberg; Fiscal Year: 2003
    ..capable of mediating resolution of infection by themselves when passively transferred into chronically infected SCID mice. Mice deficient in specific immune functions will be also be examined to determine if they ar fully or partially ..
  71. CLINICAL GENE THERAPY PROGRAM FOR THE TREATMENT OF AIDS
    Rafael Amado; Fiscal Year: 2002
    ..abstract_text> ..
  72. HAART IN SCID MICE WITH HIV ENCEPHALITIS
    William Tyor; Fiscal Year: 2003
    ..will (1) lower the viral load in brain tissue and (2) attenuate the neuropathology common to HIV-infected SCID mice. Specific Aim 1: Determine, after intraperitoneal injections, whether: (A) Mice tolerate HAART at the proposed ..
  73. Therapeutic Interventions for HIV-1 CNS Sequestration.
    Natalie Eddington; Fiscal Year: 2005
    ..uptake and activity of two "model" antiretroviral agents (AZT, zidovudine) using cell culture techniques and the SCID mice. Preliminary studies have shown that Zot is capable of reversibly opening tight junctions in the BBB, as well as ..
  74. Targeted Gold Nanoparticle-Bioconjugates for Imaging Breast Cancer
    Raghuraman Kannan; Fiscal Year: 2009
    ..utility of Bombesin conjugated AuNPs as image enhancers in computer tomographic (CT) imaging of breast tumors in SCID mice implanted with human breast cancer xenografts...
  75. MURINE MODEL OF HIV INFECTION OF HUMAN BRAIN
    Cristian Achim; Fiscal Year: 1999
    ..Pilot studies suggest that brain microspheres injected in the fat pad of SCID mice differentiate and survive for several months in vivo...
  76. Investigation of herpes simplex virus -1 neurotropism in SCID DRG xenografts
    Ann M Arvin; Fiscal Year: 2010
    ..specific aims: (1) we will define the course of events that follows HSV-1 inoculation of human DRG xenografts in SCID mice, identifying what cell types within DRG are permissive for HSV-1 gene expression, whether neurons and/or ..
  77. CANCER PATIENTS IMMUNE RESPONSES STUDIED IN SCID MICE
    Dorothee Herlyn; Fiscal Year: 1992
    ..Whereas secondary B-cell responses have been elicited in SCID mice, the suitability of this animal model for the study of T-cell responses has yet to be demonstrated...