cricetulus

Summary

Summary: A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.

Top Publications

  1. ncbi Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    R I Montgomery
    Northwestern University Medical School, Department of Microbiology Immunology, Chicago, Illinois 60611, USA
    Cell 87:427-36. 1996
  2. pmc The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
    Xun Xu
    BGI Shenzhen, Shenzhen, People s Republic of China
    Nat Biotechnol 29:735-41. 2011
  3. pmc Recreation of the terminal events in physiological integrin activation
    Feng Ye
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 188:157-73. 2010
  4. pmc Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner
    Colin K Choi
    Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 10:1039-50. 2008
  5. ncbi Reconstructing and deconstructing agonist-induced activation of integrin alphaIIbbeta3
    Jaewon Han
    Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    Curr Biol 16:1796-806. 2006
  6. pmc Hierarchy of nonhomologous end-joining, single-strand annealing and gene conversion at site-directed DNA double-strand breaks
    Wael Y Mansour
    Laboratory of Radiobiology and Experimental Radiation Oncology, Department of Radiotherapy and Radiation Oncology, University Medical School Hamburg Eppendorf, Martinistrasse 52, D 20246 Hamburg, Germany
    Nucleic Acids Res 36:4088-98. 2008
  7. pmc Formation and dissociation of M1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules
    Jonathan A Hern
    Division of Physical Biochemistry, Medical Research Council National Institute for Medical Research, London NW7 1AA, United Kingdom
    Proc Natl Acad Sci U S A 107:2693-8. 2010
  8. pmc The phasor approach to fluorescence lifetime imaging analysis
    Michelle A Digman
    Biophys J 94:L14-6. 2008
  9. pmc Transient receptor potential A1 is a sensory receptor for multiple products of oxidative stress
    David A Andersson
    Wolfson Centre for Age Related Diseases, King s College London, London SE1 1UL, United Kingdom
    J Neurosci 28:2485-94. 2008
  10. ncbi Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1
    Jun Arii
    Division of Viral Infection, Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Nature 467:859-62. 2010

Research Grants

  1. CELL TYPE/HOST ASSAY OF MUTAGEN-CARCINOGEN ACTIVITY
    George Yerganian; Fiscal Year: 1980
  2. Cell Interactions in Synapes Formation and Function
    Juan Bruses; Fiscal Year: 2005
  3. Structural determinants of human immunity to anthrax
    Donald Reason; Fiscal Year: 2008
  4. Trace Amine Receptor as Medication Development Targets
    ANITA LEWIN; Fiscal Year: 2008
  5. Human monoclonal panel mimicking anthrax immune globulin
    Donald Reason; Fiscal Year: 2005
  6. MOLECULAR DETERMINANTS OF HUMAN PNEUMOCOCCAL IMMUNITY
    Donald Reason; Fiscal Year: 2004
  7. REGULATION OF VASOPRESSIN SECRETION
    Celia D Sladek; Fiscal Year: 2010
  8. Agonist Activity at G Protein Coupled Receptors
    FRED EHLERT; Fiscal Year: 2008
  9. High Throughput Radiation Biodosimetry
    Stephen Dertinger; Fiscal Year: 2007
  10. NHLBI Bay Area Functional Genomics Consortium
    Stephen Young; Fiscal Year: 2007

Detail Information

Publications307 found, 100 shown here

  1. ncbi Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    R I Montgomery
    Northwestern University Medical School, Department of Microbiology Immunology, Chicago, Illinois 60611, USA
    Cell 87:427-36. 1996
    ..The contribution of HVEM to HSV entry into human cells was demonstrable in activated T cells. HVEM, the first identified mediator of HSV entry, is a new member of the TNF/NGF receptor family...
  2. pmc The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
    Xun Xu
    BGI Shenzhen, Shenzhen, People s Republic of China
    Nat Biotechnol 29:735-41. 2011
    ..We discuss how the availability of this genome sequence may facilitate genome-scale science for the optimization of biopharmaceutical protein production...
  3. pmc Recreation of the terminal events in physiological integrin activation
    Feng Ye
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 188:157-73. 2010
    ....
  4. pmc Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner
    Colin K Choi
    Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 10:1039-50. 2008
    ..From these studies, we have developed a model for adhesion assembly that clarifies the relative contributions of myosin II and actin polymerization and organization...
  5. ncbi Reconstructing and deconstructing agonist-induced activation of integrin alphaIIbbeta3
    Jaewon Han
    Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    Curr Biol 16:1796-806. 2006
    ..Many different signaling molecules that regulate integrin affinity have been described, but a pathway that connects agonist stimulation to talin binding and activation has not been mapped...
  6. pmc Hierarchy of nonhomologous end-joining, single-strand annealing and gene conversion at site-directed DNA double-strand breaks
    Wael Y Mansour
    Laboratory of Radiobiology and Experimental Radiation Oncology, Department of Radiotherapy and Radiation Oncology, University Medical School Hamburg Eppendorf, Martinistrasse 52, D 20246 Hamburg, Germany
    Nucleic Acids Res 36:4088-98. 2008
    ..Furthermore, the cellular choice of repair pathways is reversible and can be influenced at the level of effector proteins such as Ku80 or Rad51...
  7. pmc Formation and dissociation of M1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules
    Jonathan A Hern
    Division of Physical Biochemistry, Medical Research Council National Institute for Medical Research, London NW7 1AA, United Kingdom
    Proc Natl Acad Sci U S A 107:2693-8. 2010
    ..Two-color TIRFM established the dynamic nature of dimer formation with M(1) receptors undergoing interconversion between monomers and dimers on the timescale of seconds...
  8. pmc The phasor approach to fluorescence lifetime imaging analysis
    Michelle A Digman
    Biophys J 94:L14-6. 2008
    ....
  9. pmc Transient receptor potential A1 is a sensory receptor for multiple products of oxidative stress
    David A Andersson
    Wolfson Centre for Age Related Diseases, King s College London, London SE1 1UL, United Kingdom
    J Neurosci 28:2485-94. 2008
    ..These data demonstrate that multiple agents produced during episodes of oxidative stress can activate TRPA1 expressed in sensory neurons...
  10. ncbi Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1
    Jun Arii
    Division of Viral Infection, Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Nature 467:859-62. 2010
    ..The identification of NMHC-IIA as an HSV-1 entry receptor and the involvement of NM-IIA regulation in HSV-1 infection provide an insight into HSV-1 entry and identify new targets for antiviral drug development...
  11. pmc Overexpression of Eag1 potassium channels in clinical tumours
    Bernhard Hemmerlein
    Department of Pathology, Georg August University, Gottingen, Germany
    Mol Cancer 5:41. 2006
    ..We have now studied the expression pattern of the Eag1 channel in a large range of normal and tumour tissues from different collections utilising molecular biological and immunohistochemical techniques...
  12. ncbi Natural oligomers of the amyloid-beta protein specifically disrupt cognitive function
    James P Cleary
    Geriatric Research, Education and Clinical Center, Minneapolis Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    Nat Neurosci 8:79-84. 2005
    ....
  13. pmc The selectivity of beta-adrenoceptor agonists at human beta1-, beta2- and beta3-adrenoceptors
    Jillian G Baker
    Institute of Cell Signalling, C Floor Medical School, Queen s Medical Centre, University of Nottingham, Nottingham, UK
    Br J Pharmacol 160:1048-61. 2010
    ..This study examined the affinity and intrinsic efficacy of 31 beta-adrenoceptor agonists at the three human beta-adrenoceptors to determine whether the current agonists are subtype selective because of affinity or intrinsic efficacy...
  14. ncbi Molecular basis of xeroderma pigmentosum group C DNA recognition by engineered meganucleases
    Pilar Redondo
    Macromolecular Crystallography Group, Spanish National Cancer Research Centre CNIO, c Melchor Fdez Almagro 3, 28029 Madrid, Spain
    Nature 456:107-11. 2008
    ....
  15. pmc Mechanisms and consequences of agonist-induced talin recruitment to platelet integrin alphaIIbbeta3
    Naohide Watanabe
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 181:1211-22. 2008
    ..Thus, talin recruitment to alphaIIbbeta3 by RIAM mediates agonist-induced alphaIIbbeta3 activation, with implications for hemostasis and thrombosis...
  16. pmc Inhibition of cell migration and cell division correlates with distinct effects of microtubule inhibiting drugs
    Hailing Yang
    Department of Integrative Biology and Pharmacology, University of Texas Medical School, Houston, Texas 77030, USA
    J Biol Chem 285:32242-50. 2010
    ..We conclude that drugs produce differential dose-dependent effects at microtubule plus and minus-ends to inhibit different microtubule-mediated functions...
  17. pmc A carboxy-terminal inter-helix linker as the site of phosphatidylinositol 4,5-bisphosphate action on Kv7 (M-type) K+ channels
    Ciria C Hernandez
    Department of Physiology, MS 7756, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA
    J Gen Physiol 132:361-81. 2008
    ..The results are likely to shed light on the general structural mechanisms of phosphoinositide regulation of ion channels...
  18. ncbi Repair kinetics of DNA-DSB induced by X-rays or carbon ions under oxic and hypoxic conditions
    Ryoichi Hirayama
    Graduate School of Science and Technology, Chiba University, Yayoi cho 1 33, Inage Ku, Chiba 163 8522, Japan
    J Radiat Res 46:325-32. 2005
    ..8 and 1.8 after X-ray and carbon ion irradiations, respectively. These values matched to the OER for DNA-DSB with complete rejoining. We conclude that the rejoining of DNA-DSB is an important factor in the mechanism of the oxygen effect...
  19. pmc Complete genetic correction of ips cells from Duchenne muscular dystrophy
    Yasuhiro Kazuki
    Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Japan
    Mol Ther 18:386-93. 2010
    ..Therefore, the combination of patient-specific iPS cells and HAC-containing defective genes represents a powerful tool for gene and cell therapies...
  20. pmc Segregation and activation of myosin IIB creates a rear in migrating cells
    Miguel Vicente-Manzanares
    Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 183:543-54. 2008
    ....
  21. ncbi RBE for carbon track-segment irradiation in cell lines of differing repair capacity
    W K Weyrather
    Gesellschaft fur Schwerionenforschung, Darmstadt, Germany
    Int J Radiat Biol 75:1357-64. 1999
    ..RBE values for cell inactivation at different survival levels were determined and the differences in the RBE-LET patterns were compared with the individual sensitivity to photon irradiation of the different cell lines...
  22. ncbi Analysis of monoclonal antibody product heterogeneity resulting from alternate cleavage sites of signal peptide
    Ruchi B Kotia
    Analytical and Formulation Sciences, Amgen, Thousand Oaks, CA 91320, USA
    Anal Biochem 399:190-5. 2010
    ..The amount of the pre-LC peak recovered from the CE-SDS assay was shown to correlate with the amount of truncated peptide observed from the reduced and alkylated peptide map of the engineered mAb...
  23. pmc CD14 cooperates with complement receptor 3 to mediate MyD88-independent phagocytosis of Borrelia burgdorferi
    Kelly L Hawley
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
    Proc Natl Acad Sci U S A 109:1228-32. 2012
    ..burgdorferi that also participates in the modulation of the proinflammatory output of macrophages. These data also establish a unique mechanism of CR3-mediated phagocytosis that requires the direct cooperation of GPI-anchored proteins...
  24. pmc Structural requirements of membrane phospholipids for M-type potassium channel activation and binding
    Vsevolod Telezhkin
    Department of Neuroscience, Physiology, and Pharmacology, University College London, London WC1E 6BT, United Kingdom
    J Biol Chem 287:10001-12. 2012
    ..2 potassium channels than the more PI(4,5)P(2)-specific Kir2 channels. Notwithstanding, the data also support the view that the main endogenous activator of M-channels is PI(4,5)P(2)...
  25. ncbi Expression systems for therapeutic glycoprotein production
    Yves Durocher
    Animal Cell Technology Group, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal QC H4P2R2, Canada
    Curr Opin Biotechnol 20:700-7. 2009
    ....
  26. ncbi Optimal and consistent protein glycosylation in mammalian cell culture
    Patrick Hossler
    Process Sciences Upstream, Technical Operations, Bristol Myers Squibb Company, East Syracuse, NY 13057, USA
    Glycobiology 19:936-49. 2009
    ..This review will focus on the advancements made in glycosylation control in a manufacturing process, as well as the next steps in understanding and controlling protein glycosylation...
  27. pmc The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cells
    Christelle Rosazza
    Department of Structural Biology and Biophysics, CNRS, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France
    Mol Ther 19:913-21. 2011
    ..The disruption of the microfilaments reduces the DNA accumulation at the plasma membrane and the gene expression. This is the first direct experimental evidence of the participation of the actin cytoskeleton in DNA electrotransfer...
  28. pmc Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development
    Sonja Wilke
    Department of Molecular Structural Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany
    PLoS ONE 6:e27829. 2011
    ..The system was validated by expression, purification, deglycosylation and crystallization of the heavily glycosylated luminal domains of lysosome-associated membrane proteins (LAMP)...
  29. ncbi Bioassays for TSH-receptor autoantibodies: an update
    Simon D Lytton
    Thyroid Research Laboratory, Department of Medicine I, Gutenberg University Medical Center, Mainz 55101, Germany
    Autoimmun Rev 10:116-22. 2010
    ..The critical goal of cell-based anti-TSH-R autoantibody bioassays, to identify the pathogenic immunoglobulins in GD under robust and standardized conditions suitable for routine clinical laboratory practice, is discussed...
  30. pmc Streptolysin O clearance through sequestration into blebs that bud passively from the plasma membrane
    Peter A Keyel
    Howard Hughes Medical Institute, St Louis, MO 63110, USA
    J Cell Sci 124:2414-23. 2011
    ..We conclude, therefore, that ectocytosis is an important means for SLO clearance and hypothesize that this is a primary method by which cells defend themselves generally against pore-forming toxins...
  31. ncbi Comparative study of the cytotoxic and genotoxic effects of titanium oxide and aluminium oxide nanoparticles in Chinese hamster ovary (CHO-K1) cells
    A L Di Virgilio
    Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas, Diag 113 y 64, Correo 16, Suc 4, La Plata 1900, Argentina
    J Hazard Mater 177:711-8. 2010
    ..In conclusion, findings showed cytotoxic and genotoxic effects of TiO(2) and Al(2)O(3) NPs on CHO-K1 cells. Possible causes of controversial reports are discussed further on...
  32. ncbi Large scale microarray profiling and coexpression network analysis of CHO cells identifies transcriptional modules associated with growth and productivity
    Colin Clarke
    National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    J Biotechnol 155:350-9. 2011
    ..In summary, the approach presented in this study provides a novel perspective on the CHO cell transcriptome...
  33. pmc Kindlins, integrin activation and the regulation of talin recruitment to αIIbβ3
    Bryan N Kahner
    Department of Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 7:e34056. 2012
    ..Thus, kindlins do not promote initial talin recruitment to αIIbβ3, suggesting that they co-activate integrin through a mechanism independent of recruitment...
  34. pmc The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds
    Yi Jen Huang
    Department of Physiology and Biophysics, Miller School of Medicine, University of Miami, Miami, FL 33136, USA
    Proc Natl Acad Sci U S A 104:6436-41. 2007
    ..They also indicate a route for cell-cell communication and signal processing within the taste bud...
  35. ncbi The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production
    Mohamed B Al-Fageeh
    Protein Science Group, Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK
    Biotechnol Bioeng 93:829-35. 2006
    ..Opportunities currently exist for further enhancement of the cold-shock effect on recombinant protein production in mammalian cells through advancements in our understanding of the mechanisms involved in the cold-shock response...
  36. ncbi A microfluidic pipette for single-cell pharmacology
    Alar Ainla
    Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivagen 10, SE 412 96 Goteborg, Sweden
    Anal Chem 82:4529-36. 2010
    ..In short, the microfluidic pipette allows for complex, contamination-free multiple-compound delivery for pharmacological screening of intact adherent cells...
  37. pmc Serum-dependent selective expression of EhTMKB1-9, a member of Entamoeba histolytica B1 family of transmembrane kinases
    Shiteshu Shrimal
    School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
    PLoS Pathog 6:e1000929. 2010
    ..Our results suggest that E. histolytica TMKs of B1 family are functional kinases likely to be involved in serum response and cellular proliferation...
  38. pmc Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for cellular engineering
    Matthias Hackl
    Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 19, A 1190 Vienna, Austria
    J Biotechnol 153:62-75. 2011
    ....
  39. pmc Distinct molecular requirements for activation or stabilization of soluble guanylyl cyclase upon haem oxidation-induced degradation
    L S Hoffmann
    Pharma Research Centre, Bayer HealthCare, Aprather Weg 18a, Wuppertal, Germany
    Br J Pharmacol 157:781-95. 2009
    ....
  40. pmc Paclitaxel-dependent cell lines reveal a novel drug activity
    Anutosh Ganguly
    Department of Integrative Biology and Pharmacology, The University of Texas Medical School, 6431 Fannin St, Houston, TX 77030, USA
    Mol Cancer Ther 9:2914-23. 2010
    ..We conclude that paclitaxel rescues mutant cell division by inhibiting the detachment of microtubule minus ends from centrosomes rather than by altering plus-end microtubule dynamics...
  41. ncbi Novel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression properties
    Junhua Qiao
    Department of Molecular Biotechnology Epigenetic Regulation, Helmholtz Centre for Infection Research, Braunschweig, Germany
    J Mol Biol 390:579-94. 2009
    ..Exemplified by Chinese hamster ovary cells, the strategy proves to be successful even for cell lines with an unstable genotype...
  42. pmc The impact of intragenic CpG content on gene expression
    Asli Petra Bauer
    Institute of Medical Microbiology and Hygiene, Molecular Microbiology and Gene Therapy Unit, University of Regensburg, Regensburg, Germany
    Nucleic Acids Res 38:3891-908. 2010
    ..Increased high CpG transcription correlated with changed nucleosomal positions in vitro albeit histone density at the two genes did not change in vivo as monitored by ChIP...
  43. pmc Membrane organization and regulation of cellular cholesterol homeostasis
    María S Jaureguiberry
    Instituto de Investigaciones Bioquimicas de La Plata, CONICET UNLP, Facultad de Ciencias Medicas, calles 60 y 120, La Plata 1900, Buenos Aires, Argentina
    J Membr Biol 234:183-94. 2010
    ..The data suggest that cell functionality is preserved by regulating membrane fluidity and Chol exportation and storage...
  44. pmc The protective antigen component of anthrax toxin forms functional octameric complexes
    Alexander F Kintzer
    Department of Chemistry, University of California, Berkeley, 94720, USA
    J Mol Biol 392:614-29. 2009
    ..This assembly mechanism may provide a novel means to control cytotoxicity...
  45. ncbi E-cadherin germline missense mutations and cell phenotype: evidence for the independence of cell invasion on the motile capabilities of the cells
    Gianpaolo Suriano
    Instituto de Patologia e Immunologia Molecular da Universidade do Porto, Portugal
    Hum Mol Genet 12:3007-16. 2003
    ..Our data give new insights into the understanding of the mechanisms linking invasion and E-cadherin mutations in diffuse gastric cancer...
  46. pmc Cisternal organization of the endoplasmic reticulum during mitosis
    Lei Lu
    Department of Cell Biology and Immune Disease Institute, Harvard Medical School, Boston, MA 02115, USA
    Mol Biol Cell 20:3471-80. 2009
    ..In contrast, during interphase, the ER displays the familiar reticular network of convolved cisternae linked to tubules...
  47. ncbi Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells
    C K Leonard
    Department of Medicinal Chemistry, Genentech, Inc, South San Francisco, California 94080
    J Biol Chem 265:10373-82. 1990
    ....
  48. pmc Quantification of receptor tyrosine kinase transactivation through direct dimerization and surface density measurements in single cells
    Jody L Swift
    Departments of Chemistry and Physics, McGill University, Montreal, QC, Canada H3G 0B1
    Proc Natl Acad Sci U S A 108:7016-21. 2011
    ....
  49. ncbi Interactive competition between homologous recombination and non-homologous end joining
    Chris Allen
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Mol Cancer Res 1:913-20. 2003
    ..By blocking the two major DSB repair pathways, DNA-PKcs inhibitors should radiosensitize at all cell-cycle stages and are therefore excellent candidates for augmenting cancer radiotherapy...
  50. ncbi Complement receptor 3 blockade promotes IL-12-mediated clearance of Porphyromonas gingivalis and negates its virulence in vivo
    George Hajishengallis
    Division of Oral Health and Systemic Disease Department of Periodontics, University of Louisville Health Sciences Center, Louisville, KY 40292, USA
    J Immunol 179:2359-67. 2007
    ..Therefore, CR3 blockade may represent a promising immunomodulatory approach for controlling human periodontitis and possibly associated systemic diseases...
  51. pmc Functional UDP-xylose transport across the endoplasmic reticulum/Golgi membrane in a Chinese hamster ovary cell mutant defective in UDP-xylose Synthase
    Hans Bakker
    Zelluläre Chemie, Zentrum Biochemie, Medizinische Hochschule Hannover, Carl Neuberg Strasse 1, 30625 Hannover, Germany
    J Biol Chem 284:2576-83. 2009
    ..The data presented in this study suggest the bidirectional transport of UDP-xylose across endoplasmic reticulum/Golgi membranes and its role in controlling homeostasis of UDP-glucuronic acid and UDP-xylose production...
  52. ncbi Histone H2AX phosphorylation as a molecular pharmacological marker for DNA interstrand crosslink cancer chemotherapy
    P H Clingen
    Cancer Research UK Drug DNA Interactions Research Group, UCL Cancer Institute, University College London, 72 Huntley Street, London, WC1E 6DD, UK
    Biochem Pharmacol 76:19-27. 2008
    ..These results show that gammaH2AX can act as a highly sensitive and general marker of DNA damage induced by HN2 or cisplatin and shows promise for predicting potential cellular chemosensitivity to ICL agents...
  53. pmc Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation
    Kyohei Umebayashi
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 19:3454-62. 2008
    ..We propose that sustained tyrosine phosphorylation of EGFR facilitates its polyubiquitination in endosomes and counteracts rapid deubiquitination, thereby ensuring Hrs-dependent lysosomal sorting...
  54. pmc Leukotriene E4-induced pulmonary inflammation is mediated by the P2Y12 receptor
    Sailaja Paruchuri
    Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 206:2543-55. 2009
    ..Thus, the P2Y12 receptor is required for proinflammatory actions of the stable abundant mediator LTE4 and is a novel potential therapeutic target for asthma...
  55. pmc Functional capacity of XRCC1 protein variants identified in DNA repair-deficient Chinese hamster ovary cell lines and the human population
    Brian R Berquist
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Nucleic Acids Res 38:5023-35. 2010
    ....
  56. pmc Characterization of a binding site for anionic phospholipids on KCNQ1
    Alison M Thomas
    Department of Medicine, University College London, 5 University Street, London WC1E 6JJ, United Kingdom
    J Biol Chem 286:2088-100. 2011
    ..In conclusion, we use a combined biochemical and functional approach to identify a cluster of basic residues important for the binding and action of anionic phospholipids on the KCNQ1/KCNE1 complex...
  57. pmc Direct involvement of sigma-1 receptors in the dopamine D1 receptor-mediated effects of cocaine
    Gemma Navarro
    Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas, and Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain
    Proc Natl Acad Sci U S A 107:18676-81. 2010
    ....
  58. pmc Modulation of gamma-secretase reduces beta-amyloid deposition in a transgenic mouse model of Alzheimer's disease
    Maria Z Kounnas
    TorreyPines Therapeutics, Inc, La Jolla, CA 92037, USA
    Neuron 67:769-80. 2010
    ..These effects were observed in the absence of Notch-related changes (e.g., intestinal proliferation of goblet cells), which are commonly associated with repeated exposure to functional gamma-secretase inhibitors (GSIs)...
  59. ncbi A novel, high stringency selection system allows screening of few clones for high protein expression
    H J M van Blokland
    ChromaGenics, Kruislaan 406, 1098 SM Amsterdam, The Netherlands
    J Biotechnol 128:237-45. 2007
    ..When combined with anti-repressor elements, this novel selection system can be a valuable tool to rapidly create few, but highly productive mammalian cell lines...
  60. ncbi Robust cell line development using meganucleases
    Jean Pierre Cabaniols
    CELLECTIS S A, Romainville, France
    Methods Mol Biol 435:31-45. 2008
    ..These experiments were conducted with the natural yeast I-SceI meganuclease, but the general design and process can also be applied to engineered meganucleases...
  61. pmc Eag1 expression interferes with hypoxia homeostasis and induces angiogenesis in tumors
    Bryan R Downie
    Max Planck Institute of Experimental Medicine, Hermann Rein Str 3, 37075 Gottingen, Germany
    J Biol Chem 283:36234-40. 2008
    ..Our data suggest that Eag1 interferes with the cellular mechanism for maintaining oxygen homeostasis, increasing HIF-1 activity, and thereby VEGF secretion and tumor vascularization...
  62. ncbi The effect of triethylene glycol dimethacrylate on the cell cycle of mammalian cells
    Helmut Schweikl
    Department of Operative Dentistry and Periodontology, University of Regensburg, D 93042 Regensburg, Germany
    Biomaterials 26:4111-8. 2005
    ..From these results, we conclude that TEGDMA may influence physiological processes like cell growth and differentiation of human pulp cells in vivo...
  63. pmc Trans-dominant inhibition of prion propagation in vitro is not mediated by an accessory cofactor
    James C Geoghegan
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH, USA
    PLoS Pathog 5:e1000535. 2009
    ....
  64. ncbi Analysis of DNA ligase IV mutations found in LIG4 syndrome patients: the impact of two linked polymorphisms
    Pierre Marie Girard
    Genome Damage and Stability Centre, University of Sussex, East Sussex BN1 9RQ, UK
    Hum Mol Genet 13:2369-76. 2004
    ..Analysis of additional mutational changes in LIG4 syndrome (R580X, R814X and G469E) have led to the identification of a nuclear localization signal in DNA ligase IV and sites impacting upon DNA ligase IV adenylation...
  65. pmc Single amino acid changes in the Nipah and Hendra virus attachment glycoproteins distinguish ephrinB2 from ephrinB3 usage
    Oscar A Negrete
    Department of Microbiology, Immunology and Molecular Genetics, UCLA AIDS Institute, 609 Charles Young Dr, 3825 Molecular Science Building, Los Angeles, CA 90095, USA
    J Virol 81:10804-14. 2007
    ..Characterizing the determinants of ephrinB2 versus -B3 usage will further our understanding of henipavirus pathogenesis...
  66. pmc Molecular interplay between endostatin, integrins, and heparan sulfate
    Clément Faye
    Institut de Biologie et Chimie des Proteines, UMR 5086 CNRS University Lyon 1, IFR 128 Biosciences Gerland Lyon Sud, 7 passage du Vercors, 69367 Lyon Cedex 07, France
    J Biol Chem 284:22029-40. 2009
    ..5 nM). The direct binding between integrins and heparin/heparan sulfate might explain why both heparan sulfate and alpha5beta1 integrin are required for the localization of endostatin in endothelial cell lipid rafts...
  67. ncbi An antibody delivery system for regulated expression of therapeutic levels of monoclonal antibodies in vivo
    Jianmin Fang
    Department of Preclinical Oncology and Immunology, Cell Genesys, Inc, South San Francisco, California 94080, USA
    Mol Ther 15:1153-9. 2007
    ..In summary, this optimized mAb expression system allows regulated high-level expression of native full-length mAbs in vivo and may offer a new opportunity for delivery of therapeutic mAbs in the clinic...
  68. ncbi Inhibition of CXCR4-mediated breast cancer metastasis: a potential role for heparinoids?
    James R Harvey
    Breast Research Group, School of Surgical and Reproductive Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom
    Clin Cancer Res 13:1562-70. 2007
    ..This study was designed to test the hypothesis that soluble heparinoid glycosaminoglycan molecules can disrupt the normal response to CXCL12, thereby reducing the metastasis of CXCR4-expressing cancer cells...
  69. ncbi A heparan sulfate-facilitated and raft-dependent macropinocytosis of eosinophil cationic protein
    Tan chi Fan
    Department of Life Science, Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan 30013, China
    Traffic 8:1778-95. 2007
    ..Thus, our results define the early events of ECP internalization and may have implications for novel therapeutic design for ECP-associated diseases...
  70. ncbi The Yin Yang-1 (YY1) protein undergoes a DNA-replication-associated switch in localization from the cytoplasm to the nucleus at the onset of S phase
    Linda Palko
    Department of Biological Science, Florida State University, Tallahassee, Florida 32306 4370, USA
    J Cell Sci 117:465-76. 2004
    ..Taken together, our results provide insight into how YY1 might participate in the cell-cycle control over a variety of nuclear events required for cell division and proliferation...
  71. pmc A molecular switch that controls cell spreading and retraction
    Panagiotis Flevaris
    Department of Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA
    J Cell Biol 179:553-65. 2007
    ..Thus, calpain cleavage of beta(3) at Tyr(759) relieves c-Src-mediated RhoA inhibition, activating the RhoA pathway that confines cell spreading and causes cell retraction...
  72. ncbi Repair of DNA damage induced by accelerated heavy ions in mammalian cells proficient and deficient in the non-homologous end-joining pathway
    Ryuichi Okayasu
    International Space Radiation Laboratory, National Institute of Radiological Sciences, Inage Ku, Chiba shi 263 8555, Japan
    Radiat Res 165:59-67. 2006
    ..This delay was further emphasized in NHEJ-defective 180BR cells. Our results indicate that high-LET radiation induces complex DNA damage that is not easily repaired or is not repaired by NHEJ even at low radiation doses such as 2 Gy...
  73. ncbi A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells
    Tara L Deans
    Department of Biomedical Engineering, Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 130:363-72. 2007
    ....
  74. pmc An episomal mammalian replicon: sequence-independent binding of the origin recognition complex
    Daniel Schaarschmidt
    Department of Biology, Universitat Konstanz, Konstanz, Germany
    EMBO J 23:191-201. 2004
    ..It indicates that specific DNA sequences are not required for the chromatin binding of ORC in vivo. The conclusion is that epigenetic mechanisms determine the sites where mammalian DNA replication is initiated...
  75. pmc Establishment and mitotic stability of an extra-chromosomal mammalian replicon
    Isa M Stehle
    Institute of Cell Biology, University Witten Herdecke, Witten, Germany
    BMC Cell Biol 8:33. 2007
    ..In order to uncouple local and long-range epigenetic features we used an extra-chromosomal replicon to study the requirements for replication and segregation and compared its behavior to that of its integrated counterpart...
  76. ncbi JNK phosphorylates paxillin and regulates cell migration
    Cai Huang
    Department of Cell and Developmental Biology, Comprehensive Center for Inflammatory Disorders, University of North Carolina, Chapel Hill, North Carolina 27599 7090, USA
    Nature 424:219-23. 2003
    ..Expression of Pax(S178A) also inhibited the migration of two other cell lines. Thus, phosphorylation of paxillin by JNK seems essential for maintaining the labile adhesions required for rapid cell migration...
  77. ncbi Biosynthesis, remodelling and functions of mammalian GPI-anchored proteins: recent progress
    Taroh Kinoshita
    WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
    J Biochem 144:287-94. 2008
    ..It is thought that association with membrane rafts is important for GPI-APs in signal transduction and other functions. Here, we review recent progress in studies on biosynthesis, remodelling and functions of mammalian GPI-APs...
  78. pmc Palosuran inhibits binding to primate UT receptors in cell membranes but demonstrates differential activity in intact cells and vascular tissues
    D J Behm
    Department of Cardiovascular Pharmacology, GlaxoSmithKline, Metabolic Pathways Center of Excellence for Drug Discovery, King of Prussia, Pa 19406 0939, USA
    Br J Pharmacol 155:374-86. 2008
    ....
  79. ncbi Structural basis of integrin activation by talin
    Kate L Wegener
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England, UK
    Cell 128:171-82. 2007
    ....
  80. ncbi KCNQ1 mutation Q147R is associated with atrial fibrillation and prolonged QT interval
    Alicia Lundby
    Danish National Research Foundation Centre for Cardiac Arrhythmia, Department of Biomedical Sciences, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Heart Rhythm 4:1532-41. 2007
    ..Atrial fibrillation (AF) and long QT syndrome (LQTS) are cardiac arrhythmia disorders that have been related to dysfunction of the voltage-gated potassium channel subunit Kv7.1 encoded by the KCNQ1 gene...
  81. ncbi Induction and quantification of gamma-H2AX foci following low and high LET-irradiation
    Emma L Leatherbarrow
    DNA Damage Group, Radiation and Genome Stability Unit, Medical Research Council, Harwell, Oxfordshire, UK
    Int J Radiat Biol 82:111-8. 2006
    ....
  82. ncbi Dynamic activity of lipid droplets: protein phosphorylation and GTP-mediated protein translocation
    Rene Bartz
    Department of Cell Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9039, USA
    J Proteome Res 6:3256-65. 2007
    ....
  83. ncbi Use of the chicken lysozyme 5' matrix attachment region to generate high producer CHO cell lines
    Pierre Alain Girod
    Laboratory of Molecular Biotechnology, Center for Biotechnology UNIL EPFL, University of Lausanne, Switzerland
    Biotechnol Bioeng 91:1-11. 2005
    ..When used to express immunoglobulins, the S/MAR element enabled cell clones with high and stable levels of expression to be isolated following the analysis of a few cell lines generated without transgene amplification procedures...
  84. ncbi Familial Alzheimer's disease presenilin 1 mutations cause alterations in the conformation of presenilin and interactions with amyloid precursor protein
    Oksana Berezovska
    Alzheimer Research Unit, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    J Neurosci 25:3009-17. 2005
    ..We propose that the conformational change we observed may therefore provide a shared molecular mechanism for FAD pathogenesis caused by a wide range of PS1 mutations...
  85. ncbi Structural characterization of full-length NSF and 20S particles
    Lei Fu Chang
    State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
    Nat Struct Mol Biol 19:268-75. 2012
    ..These findings provide insight into the molecular mechanism underlying disassembly of the SNARE complex by NSF...
  86. pmc Human artificial chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells
    Jung Hyun Kim
    Laboratories of Molecular Pharmacology, National Cancer Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:20048-53. 2011
    ..This generation of human artificial chromosomes should be suitable for studies of gene function and therapeutic applications...
  87. ncbi Identification of two imidazole binding sites and key residues for substrate specificity in human primary amine oxidase AOC3
    Heli Elovaara
    MediCity Research Laboratory, University of Turku, FI 20520 Turku, Finland
    Biochemistry 50:5507-20. 2011
    ..As a result, Met211 and Leu469 were shown to be key residues for substrate specificity. The native structures of sAOC3 and the mutational data presented in this study will aid the design of hAOC3 specific inhibitors...
  88. pmc Aβ oligomers inhibit synapse remodelling necessary for memory consolidation
    Darragh B Freir
    Laboratory for Neurodegenerative Research, University College Dublin, Dublin, Ireland
    Neurobiol Aging 32:2211-8. 2011
    ..These data suggest that Aβ low-n oligomers target specific temporal facets of consolidation-associated synaptic remodelling whereby loss of functional synapses results in impaired consolidation...
  89. ncbi Early prediction of instability of Chinese hamster ovary cell lines expressing recombinant antibodies and antibody-fusion proteins
    Haimanti Dorai
    Biologics Research, Centocor R and D, 145 King of Prussia Rd, Radnor, PA 19087, USA
    Biotechnol Bioeng 109:1016-30. 2012
    ..This knowledge has been used to develop screening protocols that identify unstable CHO production cell lines at an early stage of the cell line development process, potentially reducing the cost of biotherapeutic development...
  90. pmc Structural organization of the functional domains of Clostridium difficile toxins A and B
    Rory N Pruitt
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 107:13467-72. 2010
    ....
  91. ncbi Conserved microRNAs in Chinese hamster ovary cell lines
    Kathryn C Johnson
    Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, USA
    Biotechnol Bioeng 108:475-80. 2011
    ..The identification of these miRNAs will facilitate investigations of their contribution to the hyperproductivity trait...
  92. pmc GABA, its receptors, and GABAergic inhibition in mouse taste buds
    Gennady Dvoryanchikov
    Department of Physiology and Biophysics and Program in Neuroscience, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA
    J Neurosci 31:5782-91. 2011
    ..We conclude that GABA is an inhibitory transmitter released during taste stimulation and possibly also during growth and differentiation of taste buds...
  93. pmc Cholesterol modulates the rate and mechanism of acetylcholine receptor internalization
    Virginia Borroni
    Instituto Investigaciones Bioquímicas de Bahía Blanca, C Carrindanga Km 7, B8000FWB Bahia Blanca, Argentina
    J Biol Chem 286:17122-32. 2011
    ..The Arf6-dependent mechanism may constitute the default endocytic pathway followed by the AChR in the absence of external ligands, membrane Chol levels acting as a key homeostatic regulator of cell surface receptor levels...
  94. pmc Gamma-secretase is a membrane protein complex comprised of presenilin, nicastrin, Aph-1, and Pen-2
    W Taylor Kimberly
    Center for Neurologic Diseases, Harvard Medical School and Brigham and Women s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:6382-7. 2003
    ..These findings suggest that the four membrane proteins comprise the limiting components of gamma-secretase and coassemble to form the active enzyme in mammalian cells...
  95. pmc Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane
    Mario Brameshuber
    Biophysics Institute, Johannes Kepler University Linz, Altenbergerstrasse 69, A 4040 Linz, Austria
    J Biol Chem 285:41765-71. 2010
    ....
  96. pmc Anti-heparan sulfate peptides that block herpes simplex virus infection in vivo
    Vaibhav Tiwari
    Departments of Ophthalmology and Visual Sciences, University of Illinois, Chicago, Illinois 60612, USA
    J Biol Chem 286:25406-15. 2011
    ..This result also highlights an in vivo significance of HS and 3-OS HS during ocular herpes infection...
  97. pmc The N domain of human angiotensin-I-converting enzyme: the role of N-glycosylation and the crystal structure in complex with an N domain-specific phosphinic inhibitor, RXP407
    Colin S Anthony
    Division of Medical Biochemistry, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Anzio Road, Observatory 7925, South Africa
    J Biol Chem 285:35685-93. 2010
    ..0 Å resolution. The Ndom389 structure revealed a hinge region that may contribute to the breathing motion proposed for substrate binding...
  98. pmc Human artificial chromosome with a conditional centromere for gene delivery and gene expression
    Yuichi Iida
    Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University, Nishi cho, Yonago, Tottori, Japan
    DNA Res 17:293-301. 2010
    ....
  99. ncbi The membrane repair response masks membrane disturbances caused by cell-penetrating peptide uptake
    Caroline Palm-Apergi
    Department of Neurochemistry, Stockholm University, S 106 91 Stockholm, Sweden
    FASEB J 23:214-23. 2009
    ....
  100. pmc A novel function of heparan sulfate in the regulation of cell-cell fusion
    Christopher D O'Donnell
    Department of Ophthalmology and Visual Sciences and the Department of Microbiology and Immunology, College of Medicine, University of Illinois, Chicago, Illinois 60612, USA
    J Biol Chem 284:29654-65. 2009
    ..This could serve as a possible explanation as to why HS-deficient cells showed significantly enhanced cell-cell fusion and suggests that HS could regulate fusion via fine tuning of RhoA and Cdc42 activities...
  101. ncbi IDO inhibits T-cell function through suppressing Vav1 expression and activation
    Runmei Li
    Department of Immunology, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China
    Cancer Biol Ther 8:1402-8. 2009
    ..Since Vav1 signaling pathway regulates T cell homeostasis, this study was designed to test the hypothesis that IDO induces T-cell immunosuppression through inhibiting Vav1 signaling...

Research Grants71

  1. CELL TYPE/HOST ASSAY OF MUTAGEN-CARCINOGEN ACTIVITY
    George Yerganian; Fiscal Year: 1980
    ..on the same target cell population and the same animal is presented by the autoimmune-prone Armenian hamster, Cricetulus migratorius (2n equals 22)...
  2. Cell Interactions in Synapes Formation and Function
    Juan Bruses; Fiscal Year: 2005
    ....
  3. Structural determinants of human immunity to anthrax
    Donald Reason; Fiscal Year: 2008
    ..anthracis infection. In addition, the antibodies isolated will constitute a panel of fully human monoclonal binding domains with potential for therapeutic use as passive immunogens. [unreadable] [unreadable] [unreadable]..
  4. Trace Amine Receptor as Medication Development Targets
    ANITA LEWIN; Fiscal Year: 2008
    ..Evaluation of active compounds in other neuromodulatory systems will assist in the determination of selectivity, an important consideration in medication development. [unreadable] [unreadable]..
  5. Human monoclonal panel mimicking anthrax immune globulin
    Donald Reason; Fiscal Year: 2005
    ..The PA-specific panel we construct will be molecularly defined and characterized, incorporate the advantages of toxin-specific, polyclonal human sera, and lack the inherent risk associated with blood derived products. ..
  6. MOLECULAR DETERMINANTS OF HUMAN PNEUMOCOCCAL IMMUNITY
    Donald Reason; Fiscal Year: 2004
    ..Understanding the molecular mechanisms that shape the human antibody repertoire to pneumococcal polysaccharides may also suggest strategies that would facilitate the development of more efficacious vaccines. ..
  7. REGULATION OF VASOPRESSIN SECRETION
    Celia D Sladek; Fiscal Year: 2010
    ..abstract_text> ..
  8. Agonist Activity at G Protein Coupled Receptors
    FRED EHLERT; Fiscal Year: 2008
    ..Our proposed investigation of Galpha15 and Galpha16 is highly significant with regard to the widespread use of this G protein as a transducer for both orphan and known receptors. ..
  9. High Throughput Radiation Biodosimetry
    Stephen Dertinger; Fiscal Year: 2007
    ..We propose to develop such a radiation biodosimetry assay based on the extremely high analysis rates that are possible with flow cytometry instrumentation. [unreadable] [unreadable] [unreadable]..
  10. NHLBI Bay Area Functional Genomics Consortium
    Stephen Young; Fiscal Year: 2007
    ..A fourth objective is to generate a limited number of genetically modified mice, for the purpose of defining the relevance of specific genes to cardiopulmonary development and disease (Components 4-6). ..
  11. Monitoring Oxygen Repeatedly for Hypoxic Tumor Therapy
    Mohammad Khan; Fiscal Year: 2007
    ..If successful, this approach potentially could be immediately extended for clinical applications. [unreadable] [unreadable] [unreadable]..
  12. CAAX Protein Processing and Cancer Therapy
    Stephen Young; Fiscal Year: 2007
    ..These studies should define the therapeutic potential of blocking the three different steps of CAAX protein processing. ..
  13. Novel Biological Activities of Anti-Viral Antibody
    Donald Forthal; Fiscal Year: 2006
    ..This project may provide crucial insights for guiding the development of vaccines, immunoprophylaxis and immunotherapy. ..
  14. Vasculature is a determinant of epithelial morphogenesis
    MARGARET SCHWARZ; Fiscal Year: 2006
    ..Thus, identifying the factors responsible for coordinating vascular formation and distal lung morphogenesis will provide insight into the determinants by which their balance can affect lung development. ..
  15. STRUCTURE/FUNCTION OF SURFACTANT PROTEIN D
    Erika Crouch; Fiscal Year: 2006
    ..As in the past, secreted recombinant proteins with novel structural attributes will be characterized and utilized for collaborative studies of SP-D function. [unreadable] [unreadable]..
  16. Molecular Mechanisms of FOXL2, An Ovarian Failure Gene
    Margareta Pisarska; Fiscal Year: 2010
    ..The proposed study could provide a better understanding of the mechanisms underlying premature ovarian failure. ..
  17. A NEW ANIMAL MODEL OF SCID FOR BIOMEDICAL RESEARCH
    Katheryn Meek; Fiscal Year: 2006
    ..abstract_text> ..
  18. Integrins and mechanoreception in the inflames TMJ.
    NATHANIEL JESKE; Fiscal Year: 2006
    ..These studies should generate a fundamental understanding of mechanoreceptive mechanisms involved in TMJ pain and neurogenic inflammation, potentially leading to novel therapeutics. ..
  19. Screening for Novel Modulators of Notch/RBPjk Interactions
    MA XENIA ILAGAN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  20. delta-Catenin and Cell-Cell Adhesion in Prostate Cancer
    Qun Lu; Fiscal Year: 2010
    ..Understanding the posttranslational modifications that control 8-catenin functions will unravel mechanisms by which S-catenin modulates gene expression and promotes CaP in vivo. ..
  21. Structure/Function Studies of the Integrin
    Thomas A Bunch; Fiscal Year: 2010
    ..We will pursue experiments that will address this hypothesis, potentially redefining the role of talin in integrin regulation. ..
  22. Single Agents with Designed Combination Chemotherapy Potential
    Aleem Gangjee; Fiscal Year: 2010
    ..abstract_text> ..
  23. Actions of Estrogen & Environmental Estrogens on Neurons
    Scott M Belcher; Fiscal Year: 2010
    ..The ability of ERa, ERp, and the orphan G-protein coupled receptor GPR30 to act as the membrane ER will be directly addressed. ..
  24. Antitumor Antimitotics That Reverse Tumor Resistance
    Aleem Gangjee; Fiscal Year: 2010
    ..The study will also further define the mechanism of action of the novel series and could afford agents for clinical use. ..
  25. Hutchinson Gilford Progeria Syndrome and Atherosclerosis
    Stephen Young; Fiscal Year: 2009
    ..The PI's laboratory has extensive experience in analyzing atherogenesis in mice and is thrilled with the prospect of exploring mechanisms of atherosclerosis in HGPS. ..
  26. ROLE OF SPHINGOMYELIN IN LIPOPROTEIN METABOLISM
    Papasani Subbaiah; Fiscal Year: 2008
    ..These studies will provide novel insights into the physiological role of this important phospholipid, and could possibly open up novel therapeutic targets against inflammation and atherosclerosis. ..
  27. Alpha Folate Receptor Mediated GARFTase Inhibitors as Selective Antitumor Agents
    Aleem Gangjee; Fiscal Year: 2010
    ....
  28. EFFECTS OF NONUNIFORM DISTRIBUTIONS OF RADIOACTIVITY
    Roger Howell; Fiscal Year: 2009
    ..Finally, to initiate transition of our multicellular dosimetry approach to in vivo, we will carry out bystander studies in mouse testis. ..
  29. Novel, P. jirovecii Specific Antipneumocystis Agents
    Aleem Gangjee; Fiscal Year: 2009
    ..These agents could be used alone or in combination to treat PCP thus providing novel agents against a new target. ..
  30. Strategies for mapping origins in mammalian genomes
    JOYCE HAMLIN; Fiscal Year: 2009
    ..By comparing these data to the distributions identified in Aim 1. we will define aspects of chromatin architecture that characterize active origins, origin clusters, and/or local genes. ..
  31. Cellular Physiology of Podocytes
    Stuart E Dryer; Fiscal Year: 2010
    ..Practically nothing is currently known about the effects of insulin on podocytes, which limits what we can know about diabetic nephropathy, especially in its earliest stages. ..
  32. Rapid Screen for Genotoxicants, Chemoprotectors, and Radioprotectors
    Stephen Dertinger; Fiscal Year: 2008
    ..In addition, this assay can be used to investigate agents designed to ameliorate the effects of radiation exposure, e.g. biological response modifiers. [unreadable] [unreadable] [unreadable] [unreadable]..
  33. STRUCTURE BASED ANALOGS AS ANTIOPPORTUNISTIC AGENTS
    Aleem Gangjee; Fiscal Year: 2001
    ..The analogues synthesized will also be submitted for screening against tuberculosis (NIAID) parasitic diseases (WHO) and tumor cells in culture (NCI). ..
  34. AMPLIFICATION--MODEL FOR GENETIC INSTABILITY IN CANCER
    JOYCE HAMLIN; Fiscal Year: 2005
    ..abstract_text> ..
  35. In vivo mutation assay based on pig-a locus
    Stephen Dertinger; Fiscal Year: 2004
    ....
  36. Actions of Estrogen & Environmental Estrogens on Neurons
    Scott Belcher; Fiscal Year: 2004
    ..From these results a neuronal "E2-signature cDNA array" will be developed and used in Aim 3 to determine how environmental estrogens influence E2-responsive gene expression in these developing neurons. ..
  37. Circadian Regulation of Olfaction
    STUART DRYER; Fiscal Year: 2004
    ..abstract_text> ..
  38. NOVEL NONINDUCIBLE THYMIDYLATE SYNTHASE INHIBITORS
    Aleem Gangjee; Fiscal Year: 2004
    ....
  39. Measuring Cytogenetic Damage in Human Blood
    Stephen Dertinger; Fiscal Year: 2003
    ..abstract_text> ..
  40. THE NHLBI BAY AREA FUNCTIONAL GENOMICS COMSORTIUM
    Stephen Young; Fiscal Year: 2003
    ..The Consortium's resources will be distributed freely to any interested investigator and should provide a catalyst for many different NHLBI-funded research programs. ..
  41. SUBUNIT ASSEMBLY DOMAINS--TARGETS TO LIMIT NEUROTOXICITY
    Scott Belcher; Fiscal Year: 2002
    ..abstract_text> ..
  42. FUNCTION OF LIPOPROTEIN LIPASE PROTEOGLYCAN INTERACTION
    Andre Bensadoun; Fiscal Year: 2002
    ..abstract_text> ..
  43. METABOLISM OF SPONTANEOUSLY DAMAGED PROTEINS IN AGING
    Stephen Young; Fiscal Year: 2002
    ..Finally, we propose to ask whether the expression of the repair methyltransferase in the extracellular compartment would limit the accumulation of spontaneous damage to extracellular proteins. ..
  44. ULTRASOUND-INDUCED LUNG DAMAGE ASSESSMENT
    WILLIAM O BRIEN; Fiscal Year: 2002
    ..The theoretical studies provide a mathematical basis for understanding the basic mechanism so that another approach is available for extrapolating to humans. ..
  45. DEVELOPMENT OF IONIC CHANNELS
    STUART DRYER; Fiscal Year: 2005
    ..Neurotrophic factors are potential therapeutic agents for neurodegeneration. This research will provide additional information on neurotrophic factor actions and interactions, especially with respect to posttranslational effects. ..
  46. LCAT AND PATHOPHYSIOLOGY OF PHOSPHOLIPID SPECIES
    Papasani Subbaiah; Fiscal Year: 2002
    ..These studies should provide novel insights into the physiological importance of LCAT beyond its role in cholesterol esterification. ..
  47. Study of Tritonia crawl circuit and ciliated motor cells
    OWEN WOODWARD; Fiscal Year: 2005
    ..This project may also contribute to the understanding and treatment of motor system disorders as well as demonstrate how the CNS may control non-muscular motor behaviors. ..
  48. Combinational Chemoprevention of Esophageal Adenocarcin*
    Navtej Buttar; Fiscal Year: 2005
    ..abstract_text> ..
  49. Development of EPR and NIR Oximetry for Diabetic Foot
    Mohammad Khan; Fiscal Year: 2007
    ..If the promise of these techniques is fulfilled, clinicians will have a new powerful tool for management of peripheral vascular disease, based on fundamental pathophysiology. [unreadable] [unreadable] [unreadable]..
  50. CIRCADIAN REGULATION OF VERTEBRATE PHOTORECEPTORS
    STUART DRYER; Fiscal Year: 2005
    ..abstract_text> ..
  51. Animal Models of Childhood and Adolescent Depression
    David Bylund; Fiscal Year: 2005
    ..abstract_text> ..
  52. SYNTHETIC STUDIES RELATED TO CANCER RESEARCH/TREATMENT
    Paul Wender; Fiscal Year: 2005
    ..Overall, this research program is expected to be of significant value in chemistry, biology, and medicine. ..
  53. Cannabinoid-Induced Desensitization of TRPV1 Receptors
    NATHANIEL JESKE; Fiscal Year: 2006
    ..Results from these studies will provide evidence supporting the use of cannabinoids as a possible treatment for patients suffering from local inflammatory pain. ..
  54. Third Generation Antiopportunistic Agents
    Aleem Gangjee; Fiscal Year: 2003
    ..Screening against tuberculosis (NIAID) and tumor cells in culture (NCI) is also proposed. ..
  55. HGF Signaling in Pulmonary Remodeling
    Regina Day; Fiscal Year: 2007
    ..In addition, it is expected that the results will fundamentally advance the field of lung cell biology. ..
  56. Adrenergic Receptors During Periadolescent Development
    David Bylund; Fiscal Year: 2005
    ..abstract_text> ..
  57. FANCONI ANEMIA GENE PATHWAY IN RADIATION RESPONSES
    LAWRENCE THOMPSON; Fiscal Year: 2004
    ..The results of these studies will lead to more specific models of the nature of the FA protein "pathway" and its quantitative contributions to multiple biological effects associated with IR-mediated oxidative damage. ..
  58. Postisoprenylation Processing and the Nuclear Lamina
    Stephen Young; Fiscal Year: 2007
    ..The third aim is to understand the posttranslational processing of lamin B1 and to define the consequences of lamin B1 deficiency in mammals. ..
  59. Mitochondrial Metabolic Defects and Cancer
    Nukhet Aykin Burns; Fiscal Year: 2006
    ..abstract_text> ..
  60. Automation of the vitro Micronucleus Assay
    Stephen Dertinger; Fiscal Year: 2004
    ..abstract_text> ..
  61. Versatile Mutation Assay Platform
    Stephen Dertinger; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  62. Role of a3a4a5(IV) collagen in podocyte behavior
    Corina Borza; Fiscal Year: 2005
    ..abstract_text> ..
  63. Synthesis of Cannabinoid Receptor Ligands
    John Huffman; Fiscal Year: 2005
    ..A number of new classical cannabinoids will be synthesized, including additional 1',2'-dimethylheptyl-delta8-tetrahydrocannabinol analogues and the six geminally disubstituted dimethylheptyl-delta8-tetrahydrocannabinols. ..
  64. SYNTHESIS OF CANNABINOIDS, ANALOGUES AND METABOLITES
    John Huffman; Fiscal Year: 2009
    ..There a possibility that these metabolites may be contributing to the enhanced potency of cannabinoids with a dimethylheptyl side chain. ..
  65. STUDIES ON B2-ADRENERGIC RECEPTOR MRNA BINDING PROTEIN
    BABY THOLANIKUNNEL; Fiscal Year: 2004
    ..2) To study the mechanisms by which betaAR5B protein participates in beta2AR mRNA degradation and characterize the intracellular properties of this polypeptide. ..
  66. Novel Single, Dual and Multitargeted Inhibitors of RTKs
    Aleem Gangjee; Fiscal Year: 2007
    ..abstract_text> ..
  67. The Effects of a Small Muscarinic M1 Receptor Domain on Internalization
    GREGORY SAWYER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  68. Retroviral Vectors for Safe and Efficacious Gene Therapy
    PAULA CANNON; Fiscal Year: 2004
    ..These vectors would increase the efficacy and safety of gene therapy vectors to be used in clinical trials. ..
  69. MOLECULAR CHARACTERIZATION OF C NEOFORMANS ANTIBODIES
    Arturo Casadevall; Fiscal Year: 2006
    ..These studies are expected to yield new insights on fundamental aspects of antibody function as well as practical information for the development of second generation antibody reagents for human therapy. ..