zebrafish

Summary

Summary: An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.

Top Publications

  1. pmc Efficient genome editing in zebrafish using a CRISPR-Cas system
    Woong Y Hwang
    Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Nat Biotechnol 31:227-9. 2013
  2. pmc The zebrafish reference genome sequence and its relationship to the human genome
    Kerstin Howe
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 496:498-503. 2013
  3. doi High-resolution in situ hybridization to whole-mount zebrafish embryos
    Christine Thisse
    Department of Cell Biology, University of Virginia Health Sciences Center, PO Box 800732, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908, USA
    Nat Protoc 3:59-69. 2008
  4. pmc Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos
    Nannan Chang
    Institute of Molecular Medicine, Peking University, Beijing 100871, China
    Cell Res 23:465-72. 2013
  5. pmc Optimization of a GCaMP calcium indicator for neural activity imaging
    Jasper Akerboom
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia 20147, USA
    J Neurosci 32:13819-40. 2012
  6. pmc Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs
    Lindsay Cade
    Cardiovascular Research Center, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Charlestown, MA 02129, USA
    Nucleic Acids Res 40:8001-10. 2012
  7. ncbi The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs
    Kristen M Kwan
    Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, Utah, USA
    Dev Dyn 236:3088-99. 2007
  8. ncbi In vivo imaging of embryonic vascular development using transgenic zebrafish
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD NIH, Building 6B, 6 Center Drive, Bethesda, MD 20892, USA
    Dev Biol 248:307-18. 2002
  9. pmc Systematic identification of long noncoding RNAs expressed during zebrafish embryogenesis
    Andrea Pauli
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Genome Res 22:577-91. 2012
  10. pmc Ribosome profiling shows that miR-430 reduces translation before causing mRNA decay in zebrafish
    Ariel A Bazzini
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
    Science 336:233-7. 2012

Research Grants

  1. Priyabrata Mukherjee; Fiscal Year: 2016
  2. Role of the Mammalian Siderophore in Cellular Iron Metabolism and Erythropoiesis
    Laxminarayana R Devireddy; Fiscal Year: 2012
  3. John H Postlethwait; Fiscal Year: 2016
  4. Intracellular Phase Diagram Creation Using a Micro-Differential Scanning Calorime
    GARY L SOLBREKKEN; Fiscal Year: 2010
  5. Mary K Sheats; Fiscal Year: 2016
  6. Glutamate receptor recruitment to new synapses in vivo
    PHILIP ERIC WASHBOURNE; Fiscal Year: 2013
  7. Integrated signaling of eicosanoids in HSC formation and regeneration
    Trista E North; Fiscal Year: 2013
  8. Mechanisms of Heme Transport in Macrophages
    CARINE CARINE WHITE; Fiscal Year: 2012
  9. Novel Zebrafish Models to Study Toxicity of PCBs and Pesticides During Pregnancy
    Lisa Satterwhite; Fiscal Year: 2009
  10. MECHANISMS OF EUKARYOTIC TRANSCRIPTIONAL REGULATION
    Michael R Green; Fiscal Year: 2013

Detail Information

Publications455 found, 100 shown here

  1. pmc Efficient genome editing in zebrafish using a CRISPR-Cas system
    Woong Y Hwang
    Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Nat Biotechnol 31:227-9. 2013
    ..Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like ..
  2. pmc The zebrafish reference genome sequence and its relationship to the human genome
    Kerstin Howe
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 496:498-503. 2013
    b>Zebrafish have become a popular organism for the study of vertebrate gene function...
  3. doi High-resolution in situ hybridization to whole-mount zebrafish embryos
    Christine Thisse
    Department of Cell Biology, University of Virginia Health Sciences Center, PO Box 800732, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908, USA
    Nat Protoc 3:59-69. 2008
    ..This protocol describes ISH of digoxigenin-labeled antisense RNA probes to whole-mount zebrafish embryos...
  4. pmc Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos
    Nannan Chang
    Institute of Molecular Medicine, Peking University, Beijing 100871, China
    Cell Res 23:465-72. 2013
    ..However, the applicability and efficiency of this system in model organisms, such as zebrafish, are little studied...
  5. pmc Optimization of a GCaMP calcium indicator for neural activity imaging
    Jasper Akerboom
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia 20147, USA
    J Neurosci 32:13819-40. 2012
    ..vivo in Caenorhabditis chemosensory neurons, Drosophila larval neuromuscular junction and adult antennal lobe, zebrafish retina and tectum, and mouse visual cortex. Signal-to-noise ratio was improved by at least 2- to 3-fold...
  6. pmc Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs
    Lindsay Cade
    Cardiovascular Research Center, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Charlestown, MA 02129, USA
    Nucleic Acids Res 40:8001-10. 2012
    ..Recent work has shown that TALENs can induce mutations in endogenous zebrafish genes, but to date only four genes have been altered, and larger-scale tests of the success rate, mutation ..
  7. ncbi The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs
    Kristen M Kwan
    Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, Utah, USA
    Dev Dyn 236:3088-99. 2007
    Transgenesis is an important tool for assessing gene function. In zebrafish, transgenesis has suffered from three problems: the labor of building complex expression constructs using conventional subcloning; low transgenesis efficiency, ..
  8. ncbi In vivo imaging of embryonic vascular development using transgenic zebrafish
    Nathan D Lawson
    Laboratory of Molecular Genetics, NICHD NIH, Building 6B, 6 Center Drive, Bethesda, MD 20892, USA
    Dev Biol 248:307-18. 2002
    ..We find that the zebrafish fli1 promoter is able to drive expression of enhanced green fluorescent protein (EGFP) in all blood vessels ..
  9. pmc Systematic identification of long noncoding RNAs expressed during zebrafish embryogenesis
    Andrea Pauli
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Genome Res 22:577-91. 2012
    ..in vertebrate embryogenesis, we performed a time-series of RNA-seq experiments at eight stages during early zebrafish development...
  10. pmc Ribosome profiling shows that miR-430 reduces translation before causing mRNA decay in zebrafish
    Ariel A Bazzini
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
    Science 336:233-7. 2012
    ..We monitored the impact of miR-430 on ribosome occupancy of endogenous mRNAs in wild-type and dicer mutant zebrafish embryos and found that miR-430 reduces the number of ribosomes on target mRNAs before causing mRNA decay...
  11. pmc A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish
    Philipp Niethammer
    Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 459:996-9. 2009
    ..Here we probe the role of H(2)O(2) during the early events of wound responses in zebrafish larvae expressing a genetically encoded H(2)O(2) sensor...
  12. ncbi Effective targeted gene 'knockdown' in zebrafish
    A Nasevicius
    Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Department of Genetics, Cell Biology and Development, Minneapolis, Minnesota, USA
    Nat Genet 26:216-20. 2000
    The sequencing of the zebrafish genome should be completed by the end of 2002...
  13. ncbi Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs
    Antonio J Giraldez
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    Science 312:75-9. 2006
    ..b>Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development...
  14. ncbi Animal models of human disease: zebrafish swim into view
    Graham J Lieschke
    Cancer and Haematology Division, Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, 3050, Australia
    Nat Rev Genet 8:353-67. 2007
    ..researchers who are interested in an embryologically and genetically tractable disease model have now turned to zebrafish. Zebrafish biology allows ready access to all developmental stages, and the optical clarity of embryos and ..
  15. pmc Simple methods for generating and detecting locus-specific mutations induced with TALENs in the zebrafish genome
    Timothy J Dahlem
    Department of Human Genetics, University of Utah, Salt Lake City, Utah, United States of America
    PLoS Genet 8:e1002861. 2012
    The zebrafish is a powerful experimental system for uncovering gene function in vertebrate organisms. Nevertheless, studies in the zebrafish have been limited by the approaches available for eliminating gene function...
  16. pmc In vivo genome editing using a high-efficiency TALEN system
    Victoria M Bedell
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA
    Nature 491:114-8. 2012
    The zebrafish (Danio rerio) is increasingly being used to study basic vertebrate biology and human disease with a rich array of in vivo genetic and molecular tools...
  17. pmc ZFIN: enhancements and updates to the Zebrafish Model Organism Database
    Yvonne Bradford
    ZFIN, 5291 University of Oregon, Eugene, OR 97403 5291, USA
    Nucleic Acids Res 39:D822-9. 2011
    ZFIN, the Zebrafish Model Organism Database, http://zfin.org, serves as the central repository and web-based resource for zebrafish genetic, genomic, phenotypic and developmental data...
  18. ncbi A transgenic zebrafish model of neutrophilic inflammation
    Stephen A Renshaw
    MRC Centre for Developmental and Biomedical Genetics, Acadamic Unit of Respiratory Medicine, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    Blood 108:3976-8. 2006
    We have established an in vivo model for genetic analysis of the inflammatory response by generating a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter...
  19. pmc Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
    Yannick Doyon
    Sangamo BioSciences, Inc, Pt Richmond Tech Center, 501 Canal Blvd, Suite A100, Richmond, California 94804, USA
    Nat Biotechnol 26:702-8. 2008
    We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable...
  20. pmc Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)
    Jeffry D Sander
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Nat Methods 8:67-9. 2011
    ..The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multigene pathways or genome-wide alterations...
  21. pmc Brain-wide neuronal dynamics during motor adaptation in zebrafish
    Misha B Ahrens
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Nature 485:471-7. 2012
    ..to record the activity of large populations of neurons at the cellular level, throughout the brain of larval zebrafish expressing a genetically encoded calcium sensor, while the paralysed animals interact fictively with a virtual ..
  22. pmc High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice
    Jin Hee Kim
    Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju, Republic of Korea
    PLoS ONE 6:e18556. 2011
    ..teschovirus-1, respectively, and evaluated their cleavage efficiency in three commonly used human cell lines, zebrafish embryos and adult mice...
  23. pmc Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
    Xiangdong Meng
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, 6th Floor, 364 Plantation St, Worcester, Massachusetts 01605, USA
    Nat Biotechnol 26:695-701. 2008
    ..Here we adapt this technology to create targeted mutations in the zebrafish germ line...
  24. doi Whole-brain functional imaging at cellular resolution using light-sheet microscopy
    Misha B Ahrens
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA
    Nat Methods 10:413-20. 2013
    ..through the genetically encoded calcium indicator GCaMP5G, from the entire volume of the brain of the larval zebrafish in vivo at 0.8 Hz, capturing more than 80% of all neurons at single-cell resolution...
  25. pmc Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system
    Li En Jao
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 8240, USA
    Proc Natl Acad Sci U S A 110:13904-9. 2013
    A simple and robust method for targeted mutagenesis in zebrafish has long been sought. Previous methods generate monoallelic mutations in the germ line of F0 animals, usually delaying homozygosity for the mutation to the F2 generation...
  26. pmc tp53 mutant zebrafish develop malignant peripheral nerve sheath tumors
    Stephane Berghmans
    Department of Pediatric Oncology, Dana Farber Cancer Institute, and Division of Hematology Oncology, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:407-12. 2005
    ..To further elucidate the genetic pathways involving TP53 and cancer, we have exploited the zebrafish, a powerful vertebrate model system that is amenable to whole-genome forward-genetic analysis and synthetic-..
  27. pmc p53 activation by knockdown technologies
    Mara E Robu
    University of Minnesota, Minneapolis, Minnesota, United States of America
    PLoS Genet 3:e78. 2007
    ..We have used several model genes to study these effects in detail in the zebrafish, Danio rerio...
  28. ncbi A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis
    Maxence V Nachury
    Genentech, Inc, South San Francisco, CA 94080, USA
    Cell 129:1201-13. 2007
    ..Conversely, preventing Rab8(GTP) production blocks ciliation in cells and yields characteristic BBS phenotypes in zebrafish. Our data reveal that BBS may be caused by defects in vesicular transport to the cilium.
  29. pmc Evidence for a core gut microbiota in the zebrafish
    Guus Roeselers
    Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA
    ISME J 5:1595-608. 2011
    ..We performed 16S rRNA gene sequence-based comparisons of gut bacterial communities in zebrafish collected recently from their natural habitat and those reared for generations in lab facilities in different ..
  30. pmc Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition
    Håvard Aanes
    BasAM, Norwegian School of Veterinary Science, 0033 Oslo, Norway
    Genome Res 21:1328-38. 2011
    ..To study mechanisms regulating this developmental event in zebrafish, we applied mRNA deep sequencing technology and generated comprehensive information and valuable resources on ..
  31. ncbi In vivo drug discovery in the zebrafish
    Leonard I Zon
    Howard Hughes Medical Institute, Division of Hematology Oncology, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Drug Discov 4:35-44. 2005
    The zebrafish has become a widely used model organism because of its fecundity, its morphological and physiological similarity to mammals, the existence of many genomic tools and the ease with which large, phenotype-based screens can be ..
  32. pmc Improved somatic mutagenesis in zebrafish using transcription activator-like effector nucleases (TALENs)
    Finola E Moore
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America
    PLoS ONE 7:e37877. 2012
    ..Effector Nucleases (TALENs) provide robust and user-friendly technologies for efficiently inactivating genes in zebrafish. These designer nucleases bind to and cleave DNA at particular target sites, inducing error-prone repair that ..
  33. pmc Incorporating RNA-seq data into the zebrafish Ensembl genebuild
    John E Collins
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, United Kingdom
    Genome Res 22:2067-78. 2012
    ..Two zebrafish gene annotations are presented in Ensembl version 62 built on the Zv9 reference sequence...
  34. ncbi A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish
    Koichi Kawakami
    Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Dev Cell 7:133-44. 2004
    We report here development of a novel gene trap method in zebrafish using the Tol2 transposon system...
  35. ncbi SLC24A5, a putative cation exchanger, affects pigmentation in zebrafish and humans
    Rebecca L Lamason
    Jake Gittlen Cancer Research Foundation, Department of Pathology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Science 310:1782-6. 2005
    ..Here we show that zebrafish golden mutants share these melanosomal changes and that golden encodes a putative cation exchanger slc24a5 (..
  36. pmc Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism
    Paul B Yu
    Cardiovascular Research Center and Division of Cardiology, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129, USA
    Nat Chem Biol 4:33-41. 2008
    ..which we identified in a screen for compounds that perturb dorsoventral axis formation in zebrafish. We found that dorsomorphin selectively inhibits the BMP type I receptors ALK2, ALK3 and ALK6 and thus blocks ..
  37. doi Deep sequencing of the zebrafish transcriptome response to mycobacterium infection
    Zoltán Hegedus
    Zenon Bio Ltd, Maros u 40, H 6721 Szeged, Hungary
    Mol Immunol 46:2918-30. 2009
    ..method, we investigated mycobacterium-induced transcriptome changes in a model vertebrate species, the zebrafish. We obtained a sequencing depth of over 5 million tags per sample with strong correlation between replicates...
  38. pmc Early redox, Src family kinase, and calcium signaling integrate wound responses and tissue regeneration in zebrafish
    Sa Kan Yoo
    Program in Cellular and Molecular Biology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Cell Biol 199:225-34. 2012
    ..the Src family kinase (SFK) Lyn functions as a redox sensor in leukocytes that detects H(2)O(2) at wounds in zebrafish larvae...
  39. doi Acute inflammation initiates the regenerative response in the adult zebrafish brain
    Nikos Kyritsis
    Deutsche Forshungsgemeinschaft Center for Regenerative Therapies Dresden Cluster of Excellence, Technische Universitat Dresden, Fetscherstraße 105, 01307 Dresden, Germany
    Science 338:1353-6. 2012
    The zebrafish regenerates its brain after injury and hence is a useful model organism to study the mechanisms enabling regenerative neurogenesis, which is poorly manifested in mammals...
  40. pmc Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish
    Cong Zhu
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Development 138:4555-64. 2011
    ..we evaluate a straightforward modular assembly-based approach for ZFN construction and gene inactivation in zebrafish. From an archive of 27 different zinc-finger modules, we assembled more than 70 different zinc-finger cassettes ..
  41. ncbi Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements
    Michael T Veeman
    Department of Pharmacology, Center for Developmental Biology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA 98195, USA
    Curr Biol 13:680-5. 2003
    ..To further test this hypothesis, we have identified two zebrafish homologs of the Drosophila PCP gene prickle (pk) [8], both of which show discrete and dynamic expression ..
  42. pmc DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45
    Kunal Rai
    Department of Oncological Sciences, University of Utah, Salt Lake City, UT 84112, USA
    Cell 135:1201-12. 2008
    ..Using zebrafish embryos we provide evidence for 5-methylcytosine (5-meC) removal in vivo via the coupling of a 5-meC deaminase (..
  43. pmc The role of the granuloma in expansion and dissemination of early tuberculous infection
    J Muse Davis
    Immunology and Molecular Pathogenesis Graduate Program, Emory University, Atlanta, GA 30322, USA
    Cell 136:37-49. 2009
    ..However, work in zebrafish infected with Mycobacterium marinum suggests that granulomas contribute to early bacterial growth...
  44. ncbi nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate
    J A Lister
    Department of Biological Structure, University of Washington, Box 357420, Seattle, WA 98195 7420, USA
    Development 126:3757-67. 1999
    We report the isolation and identification of a new mutation affecting pigment cell fate in the zebrafish neural crest. Homozygous nacre (nac(w2)) mutants lack melanophores throughout development but have increased numbers of iridophores...
  45. pmc Zebrafish heart regeneration occurs by cardiomyocyte dedifferentiation and proliferation
    Chris Jopling
    Center of Regenerative Medicine in Barcelona, Spain
    Nature 464:606-9. 2010
    ..contrast to mammals, several non-mammalian vertebrate species are able to regenerate their hearts, including the zebrafish, which can fully regenerate its heart after amputation of up to 20% of the ventricle...
  46. ncbi Distinct Wnt signaling pathways have opposing roles in appendage regeneration
    Cristi L Stoick-Cooper
    Howard Hughes Medical Institute, Department of Pharmacology, Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
    Development 134:479-89. 2007
    ..Using transgenic fish lines, we show that Wnt/beta-catenin signaling is activated in the regenerating zebrafish tail fin and is required for formation and subsequent proliferation of the progenitor cells of the blastema...
  47. ncbi RNA-binding protein Dnd1 inhibits microRNA access to target mRNA
    Martijn Kedde
    The Netherlands Cancer Institute, Division of Tumor Biology, Plesmanlaan 121, 1066CX, Amsterdam, The Netherlands
    Cell 131:1273-86. 2007
    ..protein (RBP), counteracts the function of several miRNAs in human cells and in primordial germ cells of zebrafish by binding mRNAs and prohibiting miRNAs from associating with their target sites...
  48. pmc The lta4h locus modulates susceptibility to mycobacterial infection in zebrafish and humans
    David M Tobin
    Department of Microbiology, University of Washington, Box 357242, Seattle, WA 98195, USA
    Cell 140:717-30. 2010
    ..Here we report results from a forward genetic screen in zebrafish larvae that identify multiple mutant classes with distinct patterns of innate susceptibility to Mycobacterium ..
  49. pmc The zebrafish transcriptome during early development
    Liselotte Vesterlund
    Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden
    BMC Dev Biol 11:30. 2011
    ..In this study RNA-Seq is used to compare the transcription profiles of four early developmental stages in zebrafish (Danio rerio) on a global scale.
  50. doi TALEN-mediated precise genome modification by homologous recombination in zebrafish
    Yao Zu
    Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing, China
    Nat Methods 10:329-31. 2013
    We report gene targeting via homologous recombination in zebrafish. We co-injected fertilized eggs with transcription activator-like effector nuclease mRNAs and a donor vector with long homologous arms targeting the tyrosine hydroxylase (..
  51. pmc Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish
    An Xiao
    Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China
    Nucleic Acids Res 41:e141. 2013
    Customized TALENs and Cas9/gRNAs have been used for targeted mutagenesis in zebrafish to induce indels into protein-coding genes...
  52. ncbi MicroRNA expression in zebrafish embryonic development
    Erno Wienholds
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Science 309:310-1. 2005
    ..We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic acid-modified oligonucleotide probes...
  53. pmc Contact inhibition of locomotion in vivo controls neural crest directional migration
    Carlos Carmona-Fontaine
    Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, UK
    Nature 456:957-61. 2008
    ..These results provide the first example of contact inhibition of locomotion in vivo, provide an explanation for coherent directional migration of groups of cells and establish a previously unknown role for non-canonical Wnt signalling...
  54. pmc Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis
    Trista E North
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 447:1007-11. 2007
    ..and homeostasis, a panel of biologically active compounds was screened for effects on stem cell induction in the zebrafish aorta-gonad-mesonephros region...
  55. pmc mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish
    Felix Ellett
    Cancer and Haematology Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
    Blood 117:e49-56. 2011
    ..Functional analyses of the cellular innate immune response in zebrafish infection/inflammation models have been aided by transgenic lines with fluorophore-marked neutrophils...
  56. doi Controlling morpholino experiments: don't stop making antisense
    Judith S Eisen
    Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403 1254, USA
    Development 135:1735-43. 2008
    ..We also discuss how the use of morpholinos can lead to misleading results, including off-target effects, and we suggest controls that will allow researchers to interpret morpholino experiments correctly...
  57. doi Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy
    Philipp J Keller
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Science 322:1065-9. 2008
    ..light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1...
  58. pmc MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis
    Stefania Nicoli
    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 464:1196-200. 2010
    ..By using two-photon imaging of live zebrafish embryos, we observe that flow is essential for angiogenesis during AA development...
  59. pmc Optogenetic dissection of a behavioural module in the vertebrate spinal cord
    Claire Wyart
    Helen Wills Neuroscience Institute and Department of Molecular and Cell Biology, University of California in Berkeley, Berkeley, California 94720, USA
    Nature 461:407-10. 2009
    ..CPG that drives spontaneous locomotion using a combination of intersectional gene expression and optogenetics in zebrafish larvae...
  60. pmc Haematopoietic stem cells derive directly from aortic endothelium during development
    Julien Y Bertrand
    Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093 0380, USA
    Nature 464:108-11. 2010
    ..We previously showed that haematopoiesis occurs through four distinct waves during zebrafish development, with HSCs arising in the final wave in close association with the dorsal aorta...
  61. pmc Multiple sex-associated regions and a putative sex chromosome in zebrafish revealed by RAD mapping and population genomics
    Jennifer L Anderson
    Institute of Neuroscience, University of Oregon, Eugene, Oregon, United States of America
    PLoS ONE 7:e40701. 2012
    Within vertebrates, major sex determining genes can differ among taxa and even within species. In zebrafish (Danio rerio), neither heteromorphic sex chromosomes nor single sex determination genes of large effect, like Sry in mammals, ..
  62. ncbi MicroRNA function in animal development
    Erno Wienholds
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    FEBS Lett 579:5911-22. 2005
    ..Here, we review the recent advances in miRNA biology in animals. Particularly, we focus on the roles of miRNAs in vertebrate development and disease...
  63. doi Control of chemokine-guided cell migration by ligand sequestration
    Bijan Boldajipour
    Center for Molecular Biology of Inflammation, Von Esmarch Strasse 56, D 48149 Munster, Germany
    Cell 132:463-73. 2008
    Primordial germ cell (PGC) migration in zebrafish is directed by the chemokine SDF-1a that activates its receptor CXCR4b. Little is known about the molecular mechanisms controlling the distribution of this chemoattractant in vivo...
  64. doi Real-time visualization of neuronal activity during perception
    Akira Muto
    Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Curr Biol 23:307-11. 2013
    ..The zebrafish is a suitable model animal for fluorescence imaging studies to visualize neuronal activity because its body is ..
  65. pmc Heritable and precise zebrafish genome editing using a CRISPR-Cas system
    Woong Y Hwang
    Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America
    PLoS ONE 8:e68708. 2013
    ..RGN) system that can be used to efficiently and robustly introduce somatic indel mutations in endogenous zebrafish genes...
  66. pmc Determinism and stochasticity during maturation of the zebrafish antibody repertoire
    Ning Jiang
    Department of Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:5348-53. 2011
    ..We used high-throughput sequencing to characterize the diversifying antibody repertoire in zebrafish over five developmental time points...
  67. doi Characterization of the mononuclear phagocyte system in zebrafish
    Valerie Wittamer
    Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093 0380, USA
    Blood 117:7126-35. 2011
    The evolutionarily conserved immune system of the zebrafish (Danio rerio), in combination with its genetic tractability, position it as an excellent model system in which to elucidate the origin and function of vertebrate immune cells...
  68. pmc The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset
    Craig J Ceol
    Stem Cell Program and Hematology Oncology, Children s Hospital Boston, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 471:513-7. 2011
    ..Here we have used a zebrafish melanoma model to test genes in a recurrently amplified region of chromosome 1 for the ability to cooperate with ..
  69. ncbi Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants
    David Traver
    Children s Hospital Boston and the Howard Hughes Medical Institute, 320 Longwood Avenue, Enders 720, Boston, Massachusetts 02115, USA
    Nat Immunol 4:1238-46. 2003
    The zebrafish is firmly established as a genetic model for the study of vertebrate blood development. Here we have characterized the blood-forming system of adult zebrafish...
  70. pmc Filtering of visual information in the tectum by an identified neural circuit
    Filippo Del Bene
    Department of Physiology, University of California, San Francisco, CA 94158, USA
    Science 330:669-73. 2010
    The optic tectum of zebrafish is involved in behavioral responses that require the detection of small objects...
  71. pmc miR-126 regulates angiogenic signaling and vascular integrity
    Jason E Fish
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Dev Cell 15:272-84. 2008
    ..Additionally, knockdown of miR-126 in zebrafish resulted in loss of vascular integrity and hemorrhage during embryonic development...
  72. doi Blood stem cells emerge from aortic endothelium by a novel type of cell transition
    Karima Kissa
    Institut Pasteur, Unité Macrophages et Développement de l Immunité and CNRS URA2578, 25 rue du Dr Roux, F 75724 Paris Cedex 15, France
    Nature 464:112-5. 2010
    ..Here we show by non-invasive, high-resolution imaging of live zebrafish embryos, that HSCs emerge directly from the aortic floor, through a stereotyped process that does not involve ..
  73. doi Dystroglycanopathies: coming into focus
    Caroline Godfrey
    Dubowitz Neuromuscular Centre, UCL Institute of Child Health and the Great Ormond Street Hospital for Children, Guilford Street, University College London, United Kingdom
    Curr Opin Genet Dev 21:278-85. 2011
    ..This review will focus on recent studies that have extended our knowledge of the mechanisms underlying dystroglycanopathies and have further characterised this patient population...
  74. pmc Interplay between Foxd3 and Mitf regulates cell fate plasticity in the zebrafish neural crest
    Kevin Curran
    Department of Biology, University of Washington, Seattle, WA 98195 7420, USA
    Dev Biol 344:107-18. 2010
    Pigment cells of the zebrafish, Danio rerio, offer an exceptionally tractable system for studying the genetic and cellular bases of cell fate decisions...
  75. ncbi Modulation of locomotor activity in larval zebrafish during light adaptation
    Harold A Burgess
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    J Exp Biol 210:2526-39. 2007
    The neural basis of behavioral choice in vertebrates remains largely unknown. Zebrafish larvae have a defined locomotor repertoire as well as a simple nervous system and are therefore an attractive vertebrate system in which to study ..
  76. pmc FGF signalling during embryo development regulates cilia length in diverse epithelia
    Judith M Neugebauer
    Department of Neurobiology and Anatomy, University of Utah School of Medicine, Eccles Institute of Human Genetics, Building 533, Room 3160, 15 North 2030 East, Salt Lake City, Utah 84112 5330, USA
    Nature 458:651-4. 2009
    ..that fibroblast growth factor (FGF) signalling regulates cilia length and function in diverse epithelia during zebrafish and Xenopus development...
  77. pmc Primary contribution to zebrafish heart regeneration by gata4(+) cardiomyocytes
    Kazu Kikuchi
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nature 464:601-5. 2010
    ..By contrast, zebrafish efficiently regenerate lost cardiac muscle, providing a model for understanding how natural heart regeneration ..
  78. pmc Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota
    John F Rawls
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63108, USA
    Proc Natl Acad Sci U S A 101:4596-601. 2004
    ..The zebrafish (Danio rerio) provides an opportunity to investigate the molecular mechanisms underlying these interactions ..
  79. pmc Dampened Hedgehog signaling but normal Wnt signaling in zebrafish without cilia
    Peng Huang
    Department of Molecular and Cellular Biology, Center for Brain Science, Harvard Stem Cell Institute, Broad Institute, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Development 136:3089-98. 2009
    ..To determine whether the role of cilia is conserved in zebrafish, we generated maternal-zygotic (MZ) oval (ovl; ift88) mutants that lack all cilia...
  80. pmc Microbial colonization induces dynamic temporal and spatial patterns of NF-κB activation in the zebrafish digestive tract
    Michelle Kanther
    Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, North Carolina, USA
    Gastroenterology 141:197-207. 2011
    ..We used in vivo imaging and molecular approaches to analyze NF-κB activation in response to the commensal microbiota in transparent gnotobiotic zebrafish.
  81. doi Bone regenerates via dedifferentiation of osteoblasts in the zebrafish fin
    Franziska Knopf
    Biotechnology Center and Center for Regenerative Therapies, University of Technology Dresden, Tatzberg 47 49, 01307 Dresden, Germany
    Dev Cell 20:713-24. 2011
    While mammals have a limited capacity to repair bone defects, zebrafish can completely regenerate amputated bony structures of their fins...
  82. doi Regeneration of the adult zebrafish brain from neurogenic radial glia-type progenitors
    Volker Kroehne
    Biotechnology Center and DFG Research Center for Regenerative Therapies Dresden, Technische Universitat Dresden, Tatzberg 47 49, 01307 Dresden, Germany
    Development 138:4831-41. 2011
    ..By contrast, several non-mammalian vertebrate species, including adult zebrafish, have a remarkable ability to regenerate injured organs, including the CNS...
  83. pmc Combinatorial roles for BMPs and Endothelin 1 in patterning the dorsal-ventral axis of the craniofacial skeleton
    Courtney Alexander
    Department of Developmental and Cell Biology, University of California, Irvine, CA 92697, USA
    Development 138:5135-46. 2011
    ..Here, we use transgenic zebrafish to monitor and perturb BMP signaling during arch formation...
  84. ncbi BRAF mutations are sufficient to promote nevi formation and cooperate with p53 in the genesis of melanoma
    E Elizabeth Patton
    Howard Hughes Medical Institute, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    Curr Biol 15:249-54. 2005
    ..To directly test the role of activated BRAF in nevus and melanoma development, we have generated transgenic zebrafish expressing the most common BRAF mutant form (V600E) under the control of the melanocyte mitfa promoter...
  85. pmc Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish
    Konstantin Stoletov
    Department of Pathology, University of California at San Diego, La Jolla 92093 0682, USA
    Circ Res 104:952-60. 2009
    ..Here we report that feeding adult zebrafish (Danio rerio) a high-cholesterol diet (HCD) resulted in hypercholesterolemia, remarkable lipoprotein oxidation, ..
  86. doi Gain and loss of function of ALS-related mutations of TARDBP (TDP-43) cause motor deficits in vivo
    Edor Kabashi
    Centre of Excellence in Neuromics, Department of Medicine, Universite de Montre al, Montreal, Quebec, Canada
    Hum Mol Genet 19:671-83. 2010
    ..TARDBP mutations (A315T, G348C and A382T) were tested in cell lines, primary cultured motor neurons and living zebrafish embryos...
  87. ncbi Neural stem cells and neurogenesis in the adult zebrafish brain: origin, proliferation dynamics, migration and cell fate
    Heiner Grandel
    Biotechnology Center and Center for Regenerative Therapies, University of Technology, Dresden, Germany
    Dev Biol 295:263-77. 2006
    ..Proliferation zones in the adult zebrafish brain are located in distinct regions along its entire anterior-posterior axis...
  88. pmc Normal table of postembryonic zebrafish development: staging by externally visible anatomy of the living fish
    David M Parichy
    Department of Biology, University of Washington, Seattle, Washington 91895, USA
    Dev Dyn 238:2975-3015. 2009
    The zebrafish is a premier model organism yet lacks a system for assigning postembryonic fish to developmental stages...
  89. pmc Tuberculous granuloma induction via interaction of a bacterial secreted protein with host epithelium
    Hannah E Volkman
    Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98155, USA
    Science 327:466-9. 2010
    ..However, analysis of Mycobacterium marinum in zebrafish has shown that the early granuloma facilitates mycobacterial growth; uninfected macrophages are recruited to the ..
  90. pmc Zebrafish behavioral profiling links drugs to biological targets and rest/wake regulation
    Jason Rihel
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 327:348-51. 2010
    ..and application of a high-throughput, quantitative screen for drugs that alter the behavior of larval zebrafish. We found that the multidimensional nature of observed phenotypes enabled the hierarchical clustering of ..
  91. pmc Transactivation from Gal4-VP16 transgenic insertions for tissue-specific cell labeling and ablation in zebrafish
    Jon M Davison
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Dev Biol 304:811-24. 2007
    Prior studies with transgenic zebrafish confirmed the functionality of the transcription factor Gal4 to drive expression of other genes under the regulation of upstream activator sequences (UAS)...
  92. pmc Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota
    Jennifer M Bates
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
    Cell Host Microbe 2:371-82. 2007
    ..Here, we show that expression of the zebrafish intestinal alkaline phosphatase (Iap), localized to the intestinal lumen brush border, is induced during ..
  93. pmc Differential regulation of protrusion and polarity by PI3K during neutrophil motility in live zebrafish
    Sa Kan Yoo
    Program in Cellular and Molecular Biology, University of Wisconsin Madison, Madison, WI 53706, USA
    Dev Cell 18:226-36. 2010
    ..The dynamics of PI(3)K products PI(3,4,5)P(3)-PI(3,4)P(2) during neutrophil migration were visualized in living zebrafish, revealing that PI(3)K activation at the leading edge is critical for neutrophil motility in intact tissues...
  94. pmc Zebrafish as a model for normal and malignant hematopoiesis
    Lili Jing
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Dis Model Mech 4:433-8. 2011
    b>Zebrafish studies in the past two decades have made major contributions to our understanding of hematopoiesis and its associated disorders...
  95. pmc The zebrafish genome encodes the largest vertebrate repertoire of functional aquaporins with dual paralogy and substrate specificities similar to mammals
    Angèle Tingaud-Sequeira
    Laboratory of Institut de Recerca i Tecnologia Agroalimentàries Institut de Ciències del Mar, Consejo Superior de Investigaciones Cientificas, 08003 Barcelona, Spain
    BMC Evol Biol 10:38. 2010
    ..In mammals, thirteen aquaporins (AQP0-12) have been characterized, but in lower vertebrates, such as fish, the diversity, structure and substrate specificity of these membrane channel proteins are largely unknown...
  96. pmc High-throughput in vivo vertebrate screening
    Carlos Pardo-Martin
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology MIT, Cambridge, Massachusetts, USA
    Nat Methods 7:634-6. 2010
    We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae...
  97. pmc A primer for morpholino use in zebrafish
    Brent R Bill
    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA
    Zebrafish 6:69-77. 2009
    Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio)...
  98. doi Immunology and zebrafish: spawning new models of human disease
    Nathan D Meeker
    The Department of Pediatrics and the Huntsman Cancer Institute, University of Utah, Suite 4265, 2000 Circle of Hope, Salt Lake City, UT 84112, USA
    Dev Comp Immunol 32:745-57. 2008
    The zebrafish has emerged as a powerful new vertebrate model of human disease. Initially prominent in developmental biology, the zebrafish has now been adopted into varied fields of study including immunology...
  99. ncbi Notch signalling limits angiogenic cell behaviour in developing zebrafish arteries
    Arndt F Siekmann
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nature 445:781-4. 2007
    ..is necessary to restrict angiogenic cell behaviour to tip cells in developing segmental arteries in the zebrafish embryo...
  100. pmc Host-microbe interactions in the developing zebrafish
    Michelle Kanther
    Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Curr Opin Immunol 22:10-9. 2010
    The amenability of the zebrafish to in vivo imaging and genetic analysis has fueled expanded use of this vertebrate model to investigate the molecular and cellular foundations of host-microbe relationships...
  101. doi Measuring anxiety in zebrafish: a critical review
    Caio Maximino
    Laboratorio de Neuroendocrinologia, Instituto de Ciencias Biologicas, Universidade Federal do Para, Belem PA, Brazil
    Behav Brain Res 214:157-71. 2010
    b>Zebrafish are increasingly being used in behavioral neuroscience, neuropsychopharmacology and neurotoxicology...

Research Grants125 found, 100 shown here

  1. Priyabrata Mukherjee; Fiscal Year: 2016
    ....
  2. Role of the Mammalian Siderophore in Cellular Iron Metabolism and Erythropoiesis
    Laxminarayana R Devireddy; Fiscal Year: 2012
    ..orthologue of entA, the gene controlling the rate-limiting step of enterobactin synthesis, in developing zebrafish embryos resulted in embryonic lethality, characterized by dramatically decreased hemoglobin levels...
  3. John H Postlethwait; Fiscal Year: 2016
    ..Work focuses on zebrafish, a genetically tractable vertebrate in which sex determination can be easily tipped in one direction or the ..
  4. Intracellular Phase Diagram Creation Using a Micro-Differential Scanning Calorime
    GARY L SOLBREKKEN; Fiscal Year: 2010
    ..tool by measuring the intracellular phase diagram of mouse oocytes, mouse and rat preimplantation embryos, and zebrafish embryos...
  5. Mary K Sheats; Fiscal Year: 2016
    ..Specific Aim 3 will be accomplished using three different animal models of inflammation: tail-wounding in zebrafish and TNFa induced cremaster injury and LPS-mediated acute lung injury in mice...
  6. Glutamate receptor recruitment to new synapses in vivo
    PHILIP ERIC WASHBOURNE; Fiscal Year: 2013
    ..including cultured hippocampal neurons, cultured cerebellar granule cells (CGCs) and spinal cord neurons in zebrafish embryos in vivo...
  7. Integrated signaling of eicosanoids in HSC formation and regeneration
    Trista E North; Fiscal Year: 2013
    ..in human leukemia, is absolutely required for HSC specification in mammals;runx1 expression is conserved in zebrafish, appearing robustly by 36 hours post fertilization (hpf) in the AGM...
  8. Mechanisms of Heme Transport in Macrophages
    CARINE CARINE WHITE; Fiscal Year: 2012
    ..In zebrafish, knockdown of hrg-1 results in yolk tube defects, hydrocephalus, and severe anemia;and these defects are fully ..
  9. Novel Zebrafish Models to Study Toxicity of PCBs and Pesticides During Pregnancy
    Lisa Satterwhite; Fiscal Year: 2009
    ..Recently we created a novel model for toxin induced HLH in zebrafish where the AHR agonist PCB-126 induces a stringy heart with a reduced ventricle and out flow tract defects ..
  10. MECHANISMS OF EUKARYOTIC TRANSCRIPTIONAL REGULATION
    Michael R Green; Fiscal Year: 2013
    ..During the past funding period we have found that Trf3-depleted zebrafish embryos exhibit multiple developmental defects and, in particular, fail to undergo hematopoiesis...
  11. BMP signaling in vertebrate development
    Ken W Y Cho; Fiscal Year: 2013
    ..a core BMP-responsive element (BRE) that responds specifically to BMP signaling from Drosophila to Xenopus, zebrafish and mouse. 2...
  12. Exon capture and large-scale sequencing for disease-cause identification, early d
    Friedhelm Hildebrandt; Fiscal Year: 2010
    ..mechanisms is particularly powerful, as they can be recapitulated in animal models by gene knockdown/knockout in zebrafish or mice...
  13. Tracie R Baker; Fiscal Year: 2016
    ..Understanding and identifying these risks will positively impact human health. My research focus is to use zebrafish to understand two toxic responses seen in adults exposed during development to 2,3,7,8 tetrachlorodibenzo-p-..
  14. PAULA GOODMAN FRAENKEL; Fiscal Year: 2015
    ..that ferroportin1 is required for iron export and iron cycling through enterocytes and macrophages in zebrafish. We have also demonstrated that the regulation of hepcidin expression in zebrafish embryos resembles that ..
  15. Lawrence C Layman; Fiscal Year: 2014
    ..expression in migratory neurons (GnRH neuronal cells and human olfactory neuroblasts) and chd7 expression in zebrafish to determine the effect upon neuron migration and the expression of key genes involved in neuron migration...
  16. A Cis-regulatory Model for Neural Border Induction
    Daniel Meulemans Medeiros; Fiscal Year: 2013
    ..In addition to testing our model, the proposed work will validate ss-catenin and SMAD antibodies for use in zebrafish embryo ChIP, and establish strategies for rapidly testing the BMP and Wnt responsiveness of novel CREs...
  17. Friedhelm Hildebrandt; Fiscal Year: 2016
    ..identified and characterized 5 additional NPHP-causing genes (NPHP5-9) and generated disease models in mouse and zebrafish. The data helped define a unifying pathogenic concept for the new disease entity of "ciliopathies", which ..
  18. Germ Cell Migration in Drosophila.
    Ruth Lehmann; Fiscal Year: 2012
    ..cell migration and survival have been studied in genetically tractable organisms such as Drosophila, mouse and zebrafish. These studies have revealed striking parallels in the behavior of germ cells and in the genes that control this ..
  19. Philippe Mourrain; Fiscal Year: 2015
    ..Last year, we found, in zebrafish and four other teleost fishes, two MCH genes: mch1 and mch2...
  20. In vivo analysis of the mechanisms of axon transport.
    Catherine M Drerup; Fiscal Year: 2011
    ..I have developed zebrafish as an in vivo model for studying axon transport by 1) developing a novel imaging approach to visualize movement ..
  21. Pbx Homeodomain Proteins in Skeletal Muscle Differentiation
    LISA A MAVES; Fiscal Year: 2011
    ..for Myod to activate Myogenin expression and the fast- twitch muscle-specific differentiation program in zebrafish embryos...
  22. Ellen Merrick Schill; Fiscal Year: 2015
    ..a non-steroidal anti-inflammatory drug (NSAID) commonly used in pregnancy, inhibited ENCC migration in a zebrafish drug screen, causing a HSCR-like distal bowel aganglionosis in affected fish...
  23. Forward Genetics to Identify Complex Gene Interactions Involved in Glaucoma
    Elena V Semina; Fiscal Year: 2010
    ..Using forward genetics in zebrafish and pedigree/patient DNA sequence analysis in humans, we will identify complex genetic interactions that ..
  24. Determinants of Disease Expression in Arrhythmogenic Cardiomyopathy
    Jeffrey E Saffitz; Fiscal Year: 2010
    ..In this Challenge Grant Application, we propose to use zebrafish as a model system to discover determinants of arrhythmogenesis and disease expression in ARVC...
  25. Thomas M Vondriska; Fiscal Year: 2016
    ..and linker histones control bulk chromatin rearrangement and global gene expression;therefore, we will employ zebrafish, isolated myocytes and mouse hearts as model systems...
  26. Identification of Disease-Causing Mutations in SCID Using Exome-Wide Sequencing
    Joseph L Roberts; Fiscal Year: 2010
    ..However, a recent report combining genome-wide SNP analysis with functional screening of candidates in zebrafish surprisingly found that mutations in the mitochondrial energy metabolism gene adenylate kinase 2 (AK2) were ..
  27. Mesonephric Model of Podocyte Injury and Regeneration
    Weibin Zhou; Fiscal Year: 2012
    ..To address these questions, I propose to establish zebrafish mesonephros as a new model system for nephrotic syndrome by: (1) utilizing the transgenic zebrafish models that ..
  28. Ruth Lehmann; Fiscal Year: 2016
    ..cell migration and survival have been studied in genetically tractable organisms such as Drosophila, mouse and zebrafish. These studies have revealed striking parallels in the behavior of germ cells and in the genes that control this ..
  29. Role of the zinc finger transcription factor ZBP89 in blood &vascular developmen
    M Amin Arnaout; Fiscal Year: 2013
    ..Forced expression of ZBP-89 increased expression of primitive and definitive hematopoiesis in zebrafish and in mouse embryonic stem cells (ESCs)/embryonic body (EB) cultures, but reduced axial and intersomitic blood ..
  30. Novel Erythroid Cell Membrane Protein
    Emery H Bresnick; Fiscal Year: 2010
    ..Aim 2 - To investigate biological functions of mFam55B via loss-of-function studies in zebrafish and mice. We identified five novel zebrafish genes with significant sequence similarity to mFam55B...
  31. Functional Assessment of the Locus for Atrial Fibrillation on Chromosome 4q25
    PATRICK THOMAS ELLINOR; Fiscal Year: 2010
    ..Using an in vivo zebrafish model system to rapidly identify conserved non-coding enhancers. 2B...
  32. Transcription Factor Cross-Talk in Developmental Toxicity
    LARISSA WILLIAMS; Fiscal Year: 2012
    ..b>Zebrafish are an excellent model to study these interactions during development because of their rapid and external ..
  33. A Developmental Craniofacial Atlas for the Zebrafish
    Charles B Kimmel; Fiscal Year: 2010
    ..We propose to construct a developmental atlas for the craniofacial skeleton of zebrafish, a widely used model organism for understanding conserved features of development of the skull, and human ..
  34. A Comparative Approach to Globin Regulation/Thalassemia
    LEONARD IRA ZON; Fiscal Year: 2012
    ..We have utilized the zebrafish as a model for studying this process...
  35. A Test of Leptin's Central Function in Zebrafish
    RICHARD LYLE LONDRAVILLE; Fiscal Year: 2012
    ..Results of these studies may lead to greater understanding of how obesity, metabolic syndrome, and diabetes develop in humans, as well as potential therapies for those diseases. ..
  36. Role of Stasimon Dysfunction in Spinal Muscular Atrophy
    Livio Pellizzoni; Fiscal Year: 2013
    ..is decreased by SMN deficiency and that contributes to SMN-dependent motor neuron dysfunction in Drosophila and zebrafish models of SMA...
  37. Small Molecule-Mediated Augmentation of Kidney Regeneration
    Neil A Hukriede; Fiscal Year: 2010
    ..screening technology to identify compounds that promote expansion of embryonic renal progenitor cells in zebrafish embryos...
  38. CHARLES G SAGERSTROM; Fiscal Year: 2015
    ..Our experiments also make novel use of several techniques - ChIP, GAL4:UAS transgenics - in zebrafish. PUBLIC HEALTH RELEVANCE: The proposed experiments are aimed at understanding hindbrain formation during ..
  39. Defining Estrogenic Regulation of Hematopoietic Stem Cell Formation and Function
    Trista E North; Fiscal Year: 2013
    ..Using murine assays, they will examine how these compounds effect HSC function across vertebrate species. ..
  40. DHVANIT INDRAVADAN SHAH; Fiscal Year: 2014
    ..The zebrafish, with its close synteny to the human genome and its conserved molecular pathways regulating the development of ..
  41. The Molecular Genetics of Early Neurogenesis
    Ethan Bier; Fiscal Year: 2013
    ..and analyze vertebrate CRMs driving differential expression of neural identity genes in the neural plate/tube of zebrafish embryos. In collaboration with Shannon Fisher's group (Univ...
  42. Iqbal Hamza; Fiscal Year: 2016
    ..The worm and human HRG-1 proteins co-localize to the endo-lysosomal compartment. Knockdown of hrg-1 in zebrafish causes hydrocephalus, yolk tube defects, and anemia - phenotypes that are fully rescued by worm HRG-1...
  43. Grigorios Oikonomou; Fiscal Year: 2015
    ..Danio rerio (zebrafish) larvae are optically transparent and have only ~10 Hcrt and ~6 LC neurons per hemisphere...
  44. Nan Gao; Fiscal Year: 2014
    ..Erfei Bi, an expert in Cdc42 and cell polarity research, Dr. Michael Pack, an expert of Zebrafish gut development, and Dr. Hiroshi Nakagawa, an expert in primary tissue culture and organotypic culture model...
  45. Genes required for lipid processing and digestive organ function in larval zebraf
    Steven A Farber; Fiscal Year: 2011
    ..metabolism that can track FAs from their site of absorption at the intestinal brush border of 6-day old zebrafish larvae, through the circulatory system, and ultimately to lipid drops within a variety of organs...
  46. Jonathan M Backer; Fiscal Year: 2015
    ..proposal addresses these questions through a focused analysis of hVps34 regulation in mammalian cells and in zebrafish. Aim 1 uses a chemical genetic approach to examine the regulation of hVps34 by the hVps15 protein kinase...
  47. Analysis of Hair Cell Regeneration in Zebrafish
    ALEJANDRO SANCHEZ ALVARADO; Fiscal Year: 2010
    ..The aim is to take advantage of the lateral line of zebrafish to define and characterize the molecular and cellular interactions occurring during hair cell regeneration with ..
  48. Embryonic regulation of GnRH neuron migration and function
    David J Kozlowski; Fiscal Year: 2012
    ..To overcome these barriers, we exploit several experimental features of zebrafish to identify GnRH neurons during embryonic migration and monitor electrophysiological activity in an intact ..
  49. Peter C Harris; Fiscal Year: 2016
    ..elegans and zebrafish employed to assess the significance of the most promising variants...
  50. JESSICA OTIS; Fiscal Year: 2014
    ..As a first step in exploring CAV1, drCav1-GFP zebrafish were created that show in vivo drCav1 localization to the basolateral and lateral plasma membranes of ..
  51. Melanin-Concentrating Hormone: Ancestral Role in Feeding & Sleep Regulation
    Philippe Mourrain; Fiscal Year: 2010
    ..Last year, we found, in zebrafish and four other teleost fishes, two MCH genes: mch1 and mch2...
  52. Joshua H Lipschutz; Fiscal Year: 2015
    ..We show in vivo correlation using sec10 antisense morpholinos in zebrafish, which results in a remarkably similar phenotype to that following injection of pkd2 morpholinos, with a "curly ..
  53. KIRSTEN C SADLER EDEPLI; Fiscal Year: 2016
    ..By forward genetic screening in zebrafish embryos combined with functional studies on liver regeneration in adults and expression analysis of human HCC ..
  54. Development and Functional Analysis of Components of the Zebrafish Serotonin Sign
    Kenneth N Wallace; Fiscal Year: 2012
    ..We will investigate the role of the serotonin signaling system to determine how it functions within the zebrafish digestive system...
  55. Weibin Zhou; Fiscal Year: 2015
    ..To address these questions, I propose to establish zebrafish mesonephros as a new model system for nephrotic syndrome by: (1) utilizing the transgenic zebrafish models that ..
  56. Roland S Wu; Fiscal Year: 2014
    ..Synthetic oligonucleotide inhibition of S1P1 expression in zebrafish leads to impaired cardiac sarcomere development. These abnormalities appear to be specific to cardiac myocytes...
  57. Genetics and visualization of innate host response to C. albicans infection in vi
    Robert T Wheeler; Fiscal Year: 2012
    ..However, our recent non-invasive imaging, using a new transparent model of disseminated Candida-zebrafish infection, indicates that macrophages in their natural context have an enhanced ability to limit proliferation ..
  58. Adam C Miller; Fiscal Year: 2014
    ..This proposal utilizes the zebrafish Mauthner (M) circuit to investigate the genetics of electrical synapse formation...
  59. Systematic functional analysis of non-coding sequences in transgenic zebrafish
    Shannon Fisher; Fiscal Year: 2010
    ..We propose to apply transposon-based transgenesis in zebrafish, to functionally analyze a large number of conserved non-coding elements in vivo...
  60. LEONARD IRA ZON; Fiscal Year: 2015
    ..We wish to exploit these new "humanized" research tools to complement our traditional expertise in zebrafish and murine models to study hematopoietic development and disease pathophysiology...
  61. "Gdf6 gene expression and evolution in vertebrates"
    Douglas Mortlock; Fiscal Year: 2009
    ..the expression and regulation of orthologs for Gdf6, an important skeletal patterning gene, during mouse and zebrafish skeletal development...
  62. Carolyn A Felix; Fiscal Year: 2014
    ..Unique attributes of zebrafish embryos enable in vivo visualization of normal and abnormal hematopoietic developmental processes in intact ..
  63. Todd R Miller; Fiscal Year: 2016
    ..In addition, a zebrafish assay will be used to detect unknown toxins and overall water toxicity...
  64. Joseph G Gleeson; Fiscal Year: 2015
    ..We will utilize both traditional and conditional knockout technologies in mouse, genetic modeling in zebrafish, and advanced live-cell imaging capability that will synergize to help advance our understanding of the ..
  65. Dale Warren Hailey; Fiscal Year: 2014
    ..I will use zebrafish lateral line hair cells because we can easily image these cells in their native context while exposing them to ..
  66. WILSON KENDRICK CLEMENTS; Fiscal Year: 2014
    ..Using zebrafish, which are transparent during embryonic development and uniquely receptive to transgenesis, allowing direct ..
  67. The role of the kimble gene in regulating craniofacial morphogenesis
    Daniel S Levic; Fiscal Year: 2013
    ..By investigating a zebrafish mutant with a malformed head skeleton, this project has identified impaired exocytosis as a potentially novel ..
  68. Stuart H Orkin; Fiscal Year: 2015
    ..was based on the premise that parallel genetic investigation of the major vertebrate model organisms, mice and zebrafish, would advance our understanding of fundamental aspects of blood cell formation, provide a platform for new ..
  69. Nancy Bettina Spinner; Fiscal Year: 2015
    ..Our mechanistic studies will utilize zebrafish, in which we have considerable experience examining hepatobiliary development...
  70. Jouni Uitto; Fiscal Year: 2016
    ..The pathogenic nature of missense mutations will also be verified in a novel zebrafish morpholino knock-down system...
  71. FLORENCE LOUISE MARLOW; Fiscal Year: 2016
    ..Studies of the genetic and molecular control of axis formation in zebrafish will clarify the mechanisms establishing these earliest oocyte asymmetries, which are conserved...
  72. KRISTIN EMILY NOACK WATT; Fiscal Year: 2015
    ..blot analysis that p53-dependent apoptosis contributes to craniofacial anomalies in polr1c and polr1d mutant zebrafish. We hypothesize that genetic inhibition of p53 in polr1c and polr1d zebrafish will prevent the pathogenesis of ..
  73. Xinchun Pi; Fiscal Year: 2016
    ..Moreover, we observe that LRP1 regulates endothelial growth, migration, inflammation and tubulogenesis in vitro, zebrafish vascular development and mouse retinal angiogenesis in vivo...
  74. REBECCA BEER; Fiscal Year: 2016
    ..The work will be supplemented with coursework in data analysis and statistics. Ultimately, the multidisciplinary nature of my postdoctoral training will propel me into my desired career as an academic PI. ..
  75. REBECCA ANN ANDERSON; Fiscal Year: 2015
    ..Therefore, zebrafish mutants displaying skeletal dysmorphologies provide an ideal system to study human craniofacial skeletogenesis ..
  76. LINDSAY T MARJORAM; Fiscal Year: 2015
    ..b>Zebrafish has proven a powerful system in modeling human disease states and an optically transparent body and digestive ..
  77. BENJAMIN J FLAHERTY; Fiscal Year: 2014
    ..co-activator TAZ through in vitro biochemical studies as well as genetic interaction experiments in zebrafish. We have identified that the PC1-CTT can bind to and stimulate the activity of TAZ...
  78. Shannon Fisher; Fiscal Year: 2016
    ..Here I propose to harness the power of the zebrafish system for genetic and live imaging studies of suture formation...
  79. Jaime Chu; Fiscal Year: 2016
    ..b>zebrafish provide a powerful vertebrate system to study monogenic disorders with their high genetic conservation to humans,..
  80. David H Sherr; Fiscal Year: 2016
    ..abstract_text> ..
  81. GWENDOLYN LEWIS; Fiscal Year: 2015
    ..In this assay, a Micropoint laser ablation system is used to transect the motor nerve in live, transgenic zebrafish, and the behaviors perineurial glia are visualized using time-lapse confocal microscopy...
  82. Philip Campbell; Fiscal Year: 2015
    ..These studies using the Zebrafish system will test the hypothesis that disrupted neuronal transport of RNAs, vesicles, and organelles represents ..
  83. Functional and Phenotypic Characterization of a New FSGS Gene
    RASHEED ADEBAYO GBADEGESIN; Fiscal Year: 2013
    ..We also showed that knockdown of anillin in zebrafish embryos disrupts the GFB...
  84. Vicente E Torres; Fiscal Year: 2016
    ..The Model Svstems Core will make available PKD model systems (C. elegans, zebrafish, and rodent) and a wide range of imaging and physiology technologies to evaluate potential therapies and ..
  85. Elena V Semina; Fiscal Year: 2014
    ..of novel factors in congenital/juvenile cataract by functional analysis of the new candidate cataract factors in zebrafish and examination of a larger group of human patients affected with cataract and related ocular conditions for ..
  86. Function and Regulation of PKA-mediated GRK Phosphorylation in Photoreceptors
    JARED DAVID CHRISPELL; Fiscal Year: 2013
    ..an appropriate model for studying the role of these two kinases in regards to human vision, we propose to use zebrafish larvae as a model to define the functional consequences of Grk1 and Grk7 phosphorylation by PKA...
  87. Niles A Pierce; Fiscal Year: 2014
    ..performance was achieved when imaging five target mRNAs simultaneously in fixed whole-mount and cross-sectioned zebrafish embryos...
  88. Anna Huttenlocher; Fiscal Year: 2015
    ..to understand the implications of these mechanisms to the pathogenesis of chronic inflammatory disease by using zebrafish as a model system to examine neutrophil chemotaxis and inflammation in vivo...
  89. Alan S Waggoner; Fiscal Year: 2016
    ..The targeted biosensor dyes will be optimized to work in both cultured endothelial cells and living zebrafish. Transgenic zebrafish will be generated that express the FAP at subcellular locations in specific cells using ..
  90. Identifying transcription factors expressed by ventral spinal cord interneurons
    KATHARINE EMMA LEWIS; Fiscal Year: 2013
    ..This project will use zebrafish embryos as a model system. Zebrafish embryos have several advantages for this area of research...
  91. CHARLES BENJAMIN LOVELY; Fiscal Year: 2014
    ..Preliminary data using zebrafish demonstrate that members of the Bmp signaling pathway are ethanol-sensitive loci...
  92. Nona Sotoodehnia; Fiscal Year: 2016
    ..mechanism of the identified genes and functionally dissect the role of the variants identified using mouse and zebrafish models...
  93. LEAH YU LIU; Fiscal Year: 2014
    ..We will use the zebrafish for our studies, which has advantages as a model organism because of in vivo imaging, time resolution, and ..
  94. Michel Bagnat; Fiscal Year: 2016
    ..Recent work in zebrafish from our laboratory has shown that notochord vacuoles are specialized lysosome-related organelles...
  95. The Role of Bicaudal-C in Polycystic Kidney Disease
    Oliver Wessely; Fiscal Year: 2013
    ..Besides mouse and rat PKD models, the more primitive pronephric kidney of Xenopus or zebrafish has emerged as an alternative model system to study the molecular mechanisms underlying the epithelial ..