burkholderia infections

Summary

Summary: Infections with bacteria of the genus BURKHOLDERIA.

Top Publications

  1. ncbi The multifarious, multireplicon Burkholderia cepacia complex
    Eshwar Mahenthiralingam
    Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK
    Nat Rev Microbiol 3:144-56. 2005
  2. ncbi Diversity and significance of Burkholderia species occupying diverse ecological niches
    Tom Coenye
    Laboratory of Microbiology, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Environ Microbiol 5:719-29. 2003
  3. ncbi The various lifestyles of the Burkholderia cepacia complex species: a tribute to adaptation
    Ludovic Vial
    INRS Institut Armand Frappier, Laval, Quebec, Canada
    Environ Microbiol 13:1-12. 2011
  4. pmc Burkholderia thailandensis as a model system for the study of the virulence-associated type III secretion system of Burkholderia pseudomallei
    Andrea Haraga
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Infect Immun 76:5402-11. 2008
  5. pmc Development of Galleria mellonella as an alternative infection model for the Burkholderia cepacia complex
    Kimberley D Seed
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
    Infect Immun 76:1267-75. 2008
  6. pmc Development of a recA gene-based identification approach for the entire Burkholderia genus
    George W Payne
    Cardiff School of Biosciences, Main Building, Museum Avenue, PO Box 915, Cardiff University, Cardiff, Wales CF10 3TL, United Kingdom
    Appl Environ Microbiol 71:3917-27. 2005
  7. ncbi Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis
    Vanitha Mariappan
    Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
    Vaccine 28:1318-24. 2010
  8. ncbi Antibiotic treatment for Burkholderia cepacia complex in people with cystic fibrosis experiencing a pulmonary exacerbation
    Alex Horsley
    School of Translational Medicine, University Hospital of South Manchester, Manchester, UK
    Cochrane Database Syst Rev 10:CD009529. 2012
  9. ncbi Burkholderia cenocepacia zinc metalloproteases influence resistance to antimicrobial peptides
    Cora Kooi
    Department of Microbiology, University of Calgary, Calgary AB T2N 4N1, Canada
    Microbiology 155:2818-25. 2009
  10. pmc BcsKC is an essential protein for the type VI secretion system activity in Burkholderia cenocepacia that forms an outer membrane complex with BcsLB
    Daniel Aubert
    Department of Microbiology and Immunology, Siebens Drake Medical Research Institute, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 285:35988-98. 2010

Research Grants

Detail Information

Publications210 found, 100 shown here

  1. ncbi The multifarious, multireplicon Burkholderia cepacia complex
    Eshwar Mahenthiralingam
    Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK
    Nat Rev Microbiol 3:144-56. 2005
    ..Here we highlight the unique characteristics of the Bcc, focusing on the factors that determine virulence...
  2. ncbi Diversity and significance of Burkholderia species occupying diverse ecological niches
    Tom Coenye
    Laboratory of Microbiology, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    Environ Microbiol 5:719-29. 2003
    ....
  3. ncbi The various lifestyles of the Burkholderia cepacia complex species: a tribute to adaptation
    Ludovic Vial
    INRS Institut Armand Frappier, Laval, Quebec, Canada
    Environ Microbiol 13:1-12. 2011
    ....
  4. pmc Burkholderia thailandensis as a model system for the study of the virulence-associated type III secretion system of Burkholderia pseudomallei
    Andrea Haraga
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Infect Immun 76:5402-11. 2008
    ..Therefore, we propose B. thailandensis as a useful BSL-1 model system to study the role of the Bsa TTSS during Burkholderia infection of mammalian cells and animals...
  5. pmc Development of Galleria mellonella as an alternative infection model for the Burkholderia cepacia complex
    Kimberley D Seed
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
    Infect Immun 76:1267-75. 2008
    ..For experimentation examining the virulent properties of the BCC, the wax worm has proven to be a useful alternative infection model...
  6. pmc Development of a recA gene-based identification approach for the entire Burkholderia genus
    George W Payne
    Cardiff School of Biosciences, Main Building, Museum Avenue, PO Box 915, Cardiff University, Cardiff, Wales CF10 3TL, United Kingdom
    Appl Environ Microbiol 71:3917-27. 2005
    ..cepacia complex strains recovered from clinical infection versus the natural environment, it did facilitate the identification of clonal strain types of B. cepacia, B. stabilis, and B. ambifaria capable of residing in both niches...
  7. ncbi Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis
    Vanitha Mariappan
    Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
    Vaccine 28:1318-24. 2010
    ..Eighteen of these immunogenic proteins which reacted with all three antibodies were identified and might be potential molecules as a diagnostic marker or a putative candidate vaccine against B. cepacia infections...
  8. ncbi Antibiotic treatment for Burkholderia cepacia complex in people with cystic fibrosis experiencing a pulmonary exacerbation
    Alex Horsley
    School of Translational Medicine, University Hospital of South Manchester, Manchester, UK
    Cochrane Database Syst Rev 10:CD009529. 2012
    ..The aim of this review is to assess the available trial evidence for choice and application of treatments for Burkholderia cepacia complex infections...
  9. ncbi Burkholderia cenocepacia zinc metalloproteases influence resistance to antimicrobial peptides
    Cora Kooi
    Department of Microbiology, University of Calgary, Calgary AB T2N 4N1, Canada
    Microbiology 155:2818-25. 2009
    ..These studies suggest that ZmpA and ZmpB may influence the resistance of B. cenocepacia to host antimicrobial peptides as well as alter the host protease/anti-protease balance in chronic respiratory infections...
  10. pmc BcsKC is an essential protein for the type VI secretion system activity in Burkholderia cenocepacia that forms an outer membrane complex with BcsLB
    Daniel Aubert
    Department of Microbiology and Immunology, Siebens Drake Medical Research Institute, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 285:35988-98. 2010
    ..Together, our data show that BcsK(C)/BcsL(B) interaction is essential for the T6SS activity in B. cenocepacia...
  11. pmc Differential mucoid exopolysaccharide production by members of the Burkholderia cepacia complex
    James E A Zlosnik
    Room 377, Child and Family Research Institute, 950 West 28th Ave, Vancouver, BC V5Z 4H4, Canada
    J Clin Microbiol 46:1470-3. 2008
    ..cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can convert from mucoid to nonmucoid...
  12. pmc Genomic acquisition of a capsular polysaccharide virulence cluster by non-pathogenic Burkholderia isolates
    Bernice Meng Qi Sim
    Genome Institute of Singapore, 60 Biopolis Street, 138672 Singapore
    Genome Biol 11:R89. 2010
    ..To study B. thailandensis genomic variation, we profiled 50 isolates using a pan-genome microarray comprising genomic elements from 28 Burkholderia strains and species...
  13. ncbi Quorum sensing in the genus Burkholderia
    Leo Eberl
    Department of Microbiology, Institute of Plant Biology, University Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Int J Med Microbiol 296:103-10. 2006
    ..Given the essential role of QS in the expression of pathogenic traits in Burkholderia these regulatory systems represent attractive targets for the development of novel therapeutics...
  14. pmc Pneumonia and septicemia caused by Burkholderia thailandensis in the United States
    Mindy B Glass
    Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, CDC, MS G34, 1600 Clifton Rd N E, Atlanta, GA 30333, USA
    J Clin Microbiol 44:4601-4. 2006
    ..It is generally considered avirulent and previously has been reported to occur only in Southeast Asia. We report the first case of pneumonia and septicemia caused by B. thailandensis in the United States...
  15. pmc Environmental Burkholderia cepacia complex isolates in human infections
    Adam Baldwin
    Warwick University, Coventry, Wales, United Kingdom
    Emerg Infect Dis 13:458-61. 2007
    ..This finding links the natural environment with the emergence of many Bcc infections...
  16. pmc Diagnostically and experimentally useful panel of strains from the Burkholderia cepacia complex
    E Mahenthiralingam
    Departments of Paediatrics and Pathology, University of British Columbia, Vancouver, British Columbia, Canada
    J Clin Microbiol 38:910-3. 2000
    ..A panel of 30 well-characterized strains representative of each genomovar and new species was assembled to assist with identification, epidemiological analysis, and virulence studies on this important group of opportunistic pathogens...
  17. ncbi Lung transplantation for patients with cystic fibrosis and Burkholderia cepacia complex infection: a single-center experience
    Anthony De Soyza
    Transplantation and Immunobiology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
    J Heart Lung Transplant 29:1395-404. 2010
    ..Therefore, many transplant centers do not list these patients for transplantation...
  18. pmc A Burkholderia cepacia complex non-ribosomal peptide-synthesized toxin is hemolytic and required for full virulence
    Euan L S Thomson
    Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada
    Virulence 3:286-98. 2012
    ..Molecular screening identifies the Bcc species containing this gene cluster, of which several strains produce hemolytic activity...
  19. ncbi Pathophysiology and management of pulmonary infections in cystic fibrosis
    Ronald L Gibson
    Department of Pediatrics, University of Washington School of Medicine, Children s Hospital, Seattle, WA 98125, USA
    Am J Respir Crit Care Med 168:918-51. 2003
    ..In addition, the article discusses infection control policies, therapies to optimize airway clearance and reduce inflammation, and potential future therapies...
  20. doi Nosocomial outbreak of two strains of Burkholderia cepacia caused by contaminated heparin
    C J Yang
    J Hosp Infect 69:398-400. 2008
  21. pmc Development of a species-specific fur gene-based method for identification of the Burkholderia cepacia complex
    Karlene H Lynch
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    J Clin Microbiol 46:447-55. 2008
    ..This assay is useful for the rapid, definitive identification of all nine current BCC species and potentially novel species groups...
  22. ncbi Outbreak of Burkholderia cepacia complex among ventilated pediatric patients linked to hospital sinks
    Cynthia A Lucero
    Epidemic Intelligence Service, Office of Workforce and Career Development, Centers for Disease Control and Prevention, Atlanta, GA, USA
    Am J Infect Control 39:775-8. 2011
    ..Hospital tap water used during oral and tracheostomy care was identified as the most likely mechanism for transmission...
  23. ncbi Approaches to measure the fitness of Burkholderia cepacia complex isolates
    C F Pope
    Centre for Clinical Microbiology, University College London, Rowland Hill Street, London NW3 2QG, UK
    J Med Microbiol 59:679-86. 2010
    ..Unlike in Pseudomonas aeruginosa where hypermutability has been detected in strains isolated from CF patients, we were unable to demonstrate hypermutability in this panel of Burkholderia cenocepacia and Burkholderia multivorans isolates...
  24. ncbi Classification and identification of the Burkholderia cepacia complex: Past, present and future
    Peter Vandamme
    Laboratory of Microbiology, K L Ledeganckstraat 35, B 9000 Ghent, Belgium
    Syst Appl Microbiol 34:87-95. 2011
    ....
  25. pmc Updated version of the Burkholderia cepacia complex experimental strain panel
    Tom Coenye
    J Clin Microbiol 41:2797-8. 2003
  26. ncbi Burkholderia cenocepacia strains isolated from cystic fibrosis patients are apparently more invasive and more virulent than rhizosphere strains
    Luisa Pirone
    ENEA C R Casaccia Department of Biotechnologies, Agroindustry and Protection of Health, Plant Genetics and Genomics Section, Rome, Italy
    Environ Microbiol 10:2773-84. 2008
    ..cenocepacia virulence, showed that they were present in B. cenocepacia strains irrespective of their origin. Overall, our results suggest that rhizosphere strains do not differ from clinical strains in some pathogenic traits...
  27. ncbi High-level resistance to fluoroquinolones and cephalosporins in Burkholderia pseudomallei and closely related species
    Dimitry V Viktorov
    Research Institute for Plague Control, Golubinskaya st 7, 400131 Volgograd, Russia
    Trans R Soc Trop Med Hyg 102:S103-10. 2008
    ....
  28. doi Outbreak of Burkholderia cepacia bacteremia caused by contaminated chlorhexidine in a hemodialysis unit
    M P Romero-Gómez
    Infect Control Hosp Epidemiol 29:377-8. 2008
  29. pmc Role of ornibactin biosynthesis in the virulence of Burkholderia cepacia: characterization of pvdA, the gene encoding L-ornithine N(5)-oxygenase
    P A Sokol
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta T2N 4N1
    Infect Immun 67:4443-55. 1999
    ..The pvdA mutants were less virulent than the parent strain in chronic and acute models of respiratory infection. A functional pvdA gene appears to be required for effective colonization and persistence in B. cepacia lung infections...
  30. pmc Intraspecies signaling involving the diffusible signal factor BDSF (cis-2-dodecenoic acid) influences virulence in Burkholderia cenocepacia
    Robert P Ryan
    BIOMERIT Research Centre, Department of Microbiology, Biosciences Institute, University College Cork, Cork, Ireland
    J Bacteriol 191:5013-9. 2009
    ..Here, we show that BDSF also acts as an intraspecies signal in B. cenocepacia to control factors contributing to virulence of this major opportunistic pathogen...
  31. pmc Contribution of Burkholderia cenocepacia flagella to infectivity and inflammation
    Teresa A Urban
    Department of Microbiology, University of Virginia Health System, Box 800734, Charlottesville, VA 22908, USA
    Infect Immun 72:5126-34. 2004
    ..cenocepacia flagella contribute to virulence in an in vivo infection model, and that induction of host immune responses through interaction with TLR5 may contribute to its overall pathogenic potential...
  32. pmc Correlation between an in vitro invasion assay and a murine model of Burkholderia cepacia lung infection
    Martin V Cieri
    Division of Infectious Disease, Children s Hospital and Regional Medical Center, Seattle, Washington 98105, USA
    Infect Immun 70:1081-6. 2002
    ..Correlation between the models for six strains was demonstrated. Our results indicate that a statistical model used to determine invasiveness in an in vitro invasion assay can be used to predict in vivo invasiveness...
  33. pmc Burkholderia cenocepacia ShvR-regulated genes that influence colony morphology, biofilm formation, and virulence
    Sujatha Subramoni
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    Infect Immun 79:2984-97. 2011
    ..Together, our data show that the ShvR-regulated genes BCAS0208 to BCAS0201 are required for the rough colony morphotype, biofilm and pellicle formation, and virulence in B. cenocepacia...
  34. pmc Burkholderia cenocepacia and Burkholderia multivorans: influence on survival in cystic fibrosis
    A M Jones
    Manchester Adult Cystic Fibrosis Centre, South Manchester University Hospitals NHS Trust, Wythenshawe Hospital, Manchester M23 9LT, UK
    Thorax 59:948-51. 2004
    ..The clinical outcome of infection with the two most commonly isolated genomovars (B cenocepacia and B multivorans) was studied at a specialist CF centre between 1982 and 2003...
  35. ncbi Diversity of the Burkholderia cepacia complex and implications for risk assessment of biological control strains
    J L Parke
    Department of Botany and Plant Pathology, Department of Crop and Soil Science, Oregon State University, Corvallis, Oregon 97331 7306, USA
    Annu Rev Phytopathol 39:225-58. 2001
    ..The capability of this bacterial complex to cause disease in plants and humans, as well as to control plant diseases, affords a rare opportunity to explore traits that may function in all three environments...
  36. pmc Agricultural use of Burkholderia (Pseudomonas) cepacia: a threat to human health?
    A Holmes
    Imperial College School of Medicine, Hammersmith Hospital, London, United Kingdom
    Emerg Infect Dis 4:221-7. 1998
    ..Therefore, the widespread agricultural use of B. cepacia should be approached with caution...
  37. ncbi An outbreak of Burkholderia cenocepacia bacteremia in immunocompromised oncology patients
    T Mann
    Infectious Diseases Unit, Sheba Medical Center, 52621, Tel Hashomer, Israel
    Infection 38:187-94. 2010
    ..We describe an outbreak of B. cenocepacia bacteremia among hospitalized oncology patients...
  38. pmc Inactivation of macrophage Rab7 by Burkholderia cenocepacia
    Kassidy K Huynh
    Division of Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada
    J Innate Immun 2:522-33. 2010
    ..Jointly, these experiments indicate that B. cenocepacia express virulence factors capable of interfering with Rab7 function and thereby with membrane traffic...
  39. pmc Antimicrobial susceptibility and synergy studies of Burkholderia cepacia complex isolated from patients with cystic fibrosis
    Juyan Zhou
    Division of Infectious Diseases, Department of Pediatrics, Columbia University, College of Physicians and Surgeons, 650 West 168th Street, PH 4 West Room 470, New York, NY 10032, USA
    Antimicrob Agents Chemother 51:1085-8. 2007
    ..Minocycline, meropenem, and ceftazidime were the most active, inhibiting 38%, 26%, and 23% of strains, respectively. Synergy was rarely noted (range, 1% to 15% of strains per antibiotic combination)...
  40. pmc Burkholderia cenocepacia ET12 strain activates TNFR1 signalling in cystic fibrosis airway epithelial cells
    Umadevi S Sajjan
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 1150 W Medical Center Drive, Ann Arbor, MI 48109, USA
    Cell Microbiol 10:188-201. 2008
    ..cenocepacia, ET12 strain. Together, these results suggest that interaction of B. cenocepacia, ET12 strain with TNFR1 may contribute to robust inflammatory responses elicited by this organism...
  41. ncbi Invasion and biofilm formation of Burkholderia dolosa is comparable with Burkholderia cenocepacia and Burkholderia multivorans
    Emma Caraher
    Department of Applied Science, National Institute of Cellular Biotechnology, Institute of Technology, Tallaght, Dublin 24, Ireland
    J Cyst Fibros 6:49-56. 2007
    ..The two most common species among the CF community are Burkholderia cenocepacia and Burkholderia multivorans. However, Burkholderia dolosa has recently been causing concern due to its transmissibility and virulence in CF patients...
  42. ncbi A napkin-associated outbreak of Burkholderia cenocepacia bacteraemia in haemodialysis patients
    G Lo Cascio
    Dipartimento di Patologia, Sezione di Microbiologia, Universita di Verona, Verona, Italy
    J Hosp Infect 64:56-62. 2006
    ..cenocepacia isolated from the napkin. In conclusion, this study found that a contaminated commercial napkin soaked in quaternary ammonium, even when quality certified, was the source of infection...
  43. ncbi Investigation of an outbreak of central venous catheter-associated bloodstream infection due to contaminated water
    Richard W Douce
    Department of Infectious Diseases, Hospital Vozandes Quito, Quito, Ecuador
    Infect Control Hosp Epidemiol 29:364-6. 2008
    ..Commercially produced ampoules of water for injection were found to be contaminated with Burkholderia cepacia and Myroides odoratus. Removal of these ampoules yielded a 10-fold reduction in the incidence of catheter-associated infection...
  44. pmc Persistence of Burkholderia multivorans within the pulmonary macrophage in the murine lung
    Karen K Chu
    Department of Pathology, University of British Columbia, Vancouver, British Columbia, Canada
    Infect Immun 72:6142-7. 2004
    ..multivorans-infected lungs localized bacteria to macrophages. Clearance of B. cenocepacia was associated with greater interleukin-1beta and neutrophil responses than the responses induced by B. multivorans...
  45. pmc Moisturizing body milk as a reservoir of Burkholderia cepacia: outbreak of nosocomial infection in a multidisciplinary intensive care unit
    Francisco Alvarez-Lerma
    Service of Intensive Care Medicine, Hospital Universitari del Mar, Universitat Autonoma de Barcelona, Passeig Maritim 25 29, E 08003 Barcelona, Spain
    Crit Care 12:R10. 2008
    ..An outbreak of severe nosocomial Burkholderia cepacia infections in patients admitted to intensive care unit (ICU), including investigation of the reservoir, is described...
  46. ncbi Characterizing uncommon Burkholderia cepacia complex isolates from an outbreak in a haemodialysis unit
    Andrea V Souza
    Centro de Pesquisa Experimental, Instituto de Ensino e Pesquisa Albert Einstein Av Albert Einstein 627, 05651 901, Sao Paulo, SP, Brazil
    J Med Microbiol 53:999-1005. 2004
    ..The genomovars recovered here were distinct from those commonly reported for cystic fibrosis isolates...
  47. ncbi Outbreak of Burkholderia cepacia bloodstream infections traced to the use of Ringer lactate solution as multiple-dose vial for catheter flushing, Phnom Penh, Cambodia
    B De Smet
    Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
    Clin Microbiol Infect 19:832-7. 2013
    ..The use of multiple-dose vials should be avoided and newly introduced procedures should be assessed for infection control risks...
  48. ncbi Case-crossover study of Burkholderia cepacia complex bloodstream infection associated with contaminated intravenous bromopride
    Ianick Souto Martins
    Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    Infect Control Hosp Epidemiol 31:516-21. 2010
    ..To investigate an outbreak of healthcare-associated Burkholderia cepacia complex (BCC) primary bloodstream infections (BCC-BSI)...
  49. pmc Species distribution and ribotype diversity of Burkholderia cepacia complex isolates from French patients with cystic fibrosis
    Sylvain Brisse
    Unité Biodiversité des Bactéries Pathogènes Emergents U389 INSERM, Institut Pasteur Paris, France
    J Clin Microbiol 42:4824-7. 2004
    ..B. multivorans genotype A (found in 32 French patients) and two other genotypes were also identified among isolates from Austrian, German, Italian, and Canadian patients...
  50. pmc Mutational analysis of Burkholderia thailandensis quorum sensing and self-aggregation
    Josephine R Chandler
    Department of Microbiology, University of Washington School of Medicine, Seattle, WA 98195 7242, USA
    J Bacteriol 191:5901-9. 2009
    ..We propose that the key function of the BtaR1-BtaI1 quorum-sensing system is to cause cells to gather into aggregates once a sufficient population has been established...
  51. pmc Colonial morphology of Burkholderia cepacia complex genomovar III: implications in exopolysaccharide production, pilus expression, and persistence in the mouse
    Jacqueline W Chung
    Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada
    Infect Immun 71:904-9. 2003
    ..Colonial morphology in the BCC strain variant was associated with persistence after pulmonary challenge and appeared to be correlated with the elaboration of putative virulence determinants...
  52. pmc Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis
    Pavel Drevinek
    Department of Pediatrics, 2nd Medical School, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic
    J Clin Microbiol 48:1888-91. 2010
    ..The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum samples, of which 8 were culture negative...
  53. pmc Differential intracellular fate of Burkholderia pseudomallei 844 and Burkholderia thailandensis UE5 in human monocyte-derived dendritic cells and macrophages
    Jaruek Charoensap
    Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand
    BMC Immunol 10:20. 2009
    ..pseudomallei strain 844 (Bp-844) in human monocyte-derived dendritic cells (MoDCs) and macrophages (Mphis), as well as its ability to stimulate host cell responses with those of B. thailandensis strain UE5 (Bt-UE5)...
  54. pmc Experimental bacteriophage therapy increases survival of Galleria mellonella larvae infected with clinically relevant strains of the Burkholderia cepacia complex
    Kimberley D Seed
    Department of Biological Sciences, University of Alberta, M354 Biological Sciences Building, Edmonton, Alberta, Canada T6G 2E9
    Antimicrob Agents Chemother 53:2205-8. 2009
    ..In a caterpillar infection model, we show that BCC phage therapy is an alternative treatment possibility and is highly effective under specific conditions...
  55. ncbi Outbreak of Burkholderia cepacia bloodstream infection at an outpatient hematology and oncology practice
    Karon Abe
    Division of Public Health, Georgia Department of Human Resources, Atlanta, GA, USA
    Infect Control Hosp Epidemiol 28:1311-3. 2007
    ..Though we did not identify the source of the organism, our findings support the hypothesis that cross-contamination of multidose medications through the use of the same needle and syringe was a contributing factor...
  56. ncbi Hospital-wide outbreak of Burkholderia contaminans caused by prefabricated moist washcloths
    M Martin
    University of Lubeck, Institute for Medical Microbiology and Hygiene, Lubeck, Germany
    J Hosp Infect 77:267-70. 2011
    ..In case of infections due to contaminated products, immediate communication to healthcare authorities is required, including RAPEX warning if products are sold across Europe...
  57. pmc Outbreak of subclinical mastitis in a flock of dairy sheep associated with Burkholderia cepacia complex infection
    E Berriatua
    Sanidad Animal, Instituto Vasco de Investigacion y Desarrollo Agrario NEIKER, 48160 Derio, Bizkaia, Spain
    J Clin Microbiol 39:990-4. 2001
    ..cepacia" in any of the samples taken. To our knowledge, this is the first report of an outbreak of natural infection in animals caused by B. cepacia complex and the first description of B. cepacia complex infection in sheep...
  58. ncbi Molecular characterization of Burkholderia cepacia isolates from cystic fibrosis (CF) patients in an Italian CF center
    Andrea Petrucca
    Azienda Ospedaliera Sant Andrea, Laboratorio di Microbiologia Clinica, Via di Grottarossa 1035, 00189 Rome, Italy
    Res Microbiol 154:491-8. 2003
    ..cepacia complex strains were determined and probable patient-to-patient spread was observed...
  59. pmc Potential mechanisms underlying the acute lung dysfunction and bacterial extrapulmonary dissemination during Burkholderia cenocepacia respiratory infection
    Luiz G Cunha
    Departamento de Microbiologia, Faculdade de Ciencias Medicas, Universidade do Estado do Rio de Janeiro, Brazil
    Respir Res 11:4. 2010
    ..cenocepacia from the ET-12 lineage, that has been linked to the cepacia syndrome, and four clinical isolates recovered from CF patients with mild clinical courses were analysed in both in vitro and in vivo assays...
  60. ncbi Pseudomonas aeruginosa and Burkholderia cenocepacia infections in patients affected by cystic fibrosis: serum resistance and antibody response
    D Savoia
    Department of Clinical and Biological Sciences, University of Torino, Orbassano, Italy
    Immunol Invest 37:19-27. 2008
    ..aeruginosa was higher than that against B. cenocepacia. The contribution of these data to the assessment of the importance of the humoral immune response in CF pulmonary infections by Pseudomonas and Burkholderia is briefly discussed...
  61. ncbi Survival after lung transplantation of cystic fibrosis patients infected with Burkholderia cepacia complex
    B D Alexander
    Division of Infectious Diseases and International Health, Department of Medicine, Duke University School of Medicine, Durham, NC, USA
    Am J Transplant 8:1025-30. 2008
    ..00005). Following LT, infection with Bcc species other than B. cenocepacia does not significantly impact 5-year survival whereas infection with B. cenocepacia pretransplant is associated with decreased survival...
  62. pmc The Burkholderia cepacia epidemic strain marker is part of a novel genomic island encoding both virulence and metabolism-associated genes in Burkholderia cenocepacia
    Adam Baldwin
    Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, Wales, Canada TN2 4N1
    Infect Immun 72:1537-47. 2004
    ....
  63. pmc Toll-like receptor signaling in airborne Burkholderia thailandensis infection
    T Eoin West
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington School of Medicine, Seattle, Washington 98104, USA
    Infect Immun 77:5612-22. 2009
    ..TLR signaling in B. thailandensis infection is substantially comparable to signaling induced by virulent B. pseudomallei. These studies provide additional insights into the host-pathogen interaction in pneumonic Burkholderia infection...
  64. ncbi Epidemiology of Burkholderia cepacia complex colonisation in cystic fibrosis patients
    K De Boeck
    University Hospital Gasthuisberg, Leuven, Belgium
    Eur Respir J 23:851-6. 2004
    ..The cross-infection rate is low. Three patients had transient colonisation. The impact of Burkholderia cepacia complex on morbidity in the Belgian cystic fibrosis population is high and not limited to Burkholderia cenocepacia...
  65. ncbi Immunolocalisation of Burkholderia cepacia in the lungs of cystic fibrosis patients
    U Sajjan
    Department of Structural Biology and Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada
    J Med Microbiol 50:535-46. 2001
    ..These results suggest that B. cepacia has the capacity to be highly invasive, migrating from the airways across the epithelial barrier to invade the lung parenchyma and capillaries, thereby initiating septicaemia...
  66. pmc Reconstitution of O-specific lipopolysaccharide expression in Burkholderia cenocepacia strain J2315, which is associated with transmissible infections in patients with cystic fibrosis
    Ximena Ortega
    Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada N6A 5C1
    J Bacteriol 187:1324-33. 2005
    ....
  67. pmc A type IV secretion system contributes to intracellular survival and replication of Burkholderia cenocepacia
    S Umadevi Sajjan
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Infect Immun 76:5447-55. 2008
    ..cenocepacia is similar in both professional and nonprofessional phagocytes and that a functional plasmid-encoded type IV secretion system contributes to the survival and replication of B. cenocepacia in eukaryotic cells...
  68. pmc Identification of potential therapeutic targets for Burkholderia cenocepacia by comparative transcriptomics
    Deborah R Yoder-Himes
    Center for Microbial Ecology, Michigan State University, East Lansing, Michigan, United States of America
    PLoS ONE 5:e8724. 2010
    ..The identification of virulence factors and potential therapeutic targets has been hampered by the genomic diversity within the species as many factors are not shared among the pathogenic members of the species...
  69. pmc Efficacy of bacteriophage therapy in a model of Burkholderia cenocepacia pulmonary infection
    Lisa A Carmody
    Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Infect Dis 201:264-71. 2010
    ..cenocepacia lung infection model. Systemic phage administration was more effective than inhalational administration, suggesting that circulating phages have better access to bacteria in lungs than do topical phages...
  70. ncbi Burkholderia cepacia complex: epithelial cell-pathogen confrontations and potential for therapeutic intervention
    Siobhan McClean
    Centre of Microbial Host Interactions, Institute of Technology Tallaght Dublin, Dublin 24, Ireland
    J Med Microbiol 58:1-12. 2009
    ..This review outlines these interactions and examines the therapeutic implications of understanding the mechanisms of pathogenesis of this difficult, antibiotic-resistant, opportunistic pathogen...
  71. pmc CpG-modified plasmid DNA encoding flagellin improves immunogenicity and provides protection against Burkholderia pseudomallei infection in BALB/c mice
    Yao Shen Chen
    Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, Taiwan
    Infect Immun 74:1699-705. 2006
    ..pseudomallei infection. This CpG-modified plasmid DNA vaccination is an important potential strategy that should be developed to protect against melioidosis...
  72. pmc Role of bacteriocins in mediating interactions of bacterial isolates taken from cystic fibrosis patients
    Suphan Bakkal
    Department of Biology, University of Massachusetts Amherst, 611 North Pleasant Street, Amherst, MA 01003, USA
    Microbiology 156:2058-67. 2010
    ..These data suggest that bacteriocin-based inhibition may play a role in governing Pa and Bcc interactions in the CF lung and may, therefore, offer a novel approach to mediating these often fatal infections...
  73. ncbi A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins
    Ashraful Haque
    London School of Hygiene and Tropical Medicine, London, United Kingdom
    J Infect Dis 194:1241-8. 2006
    ..CD4+ and CD8+ cell depletion studies demonstrated that CD4+ cells, but not CD8+ cells, mediated this protection in vivo. Thus, CD4+ T cells can mediate vaccine-induced immunity to experimental melioidosis...
  74. pmc Pseudomonas aeruginosa alginate promotes Burkholderia cenocepacia persistence in cystic fibrosis transmembrane conductance regulator knockout mice
    Sangbrita S Chattoraj
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109, USA
    Infect Immun 78:984-93. 2010
    ..These observations suggest that P. aeruginosa alginate may facilitate B. cenocepacia infection by interfering with host innate defense mechanisms...
  75. pmc Multilocus sequence typing breathes life into a microbial metagenome
    Eshwar Mahenthiralingam
    Cardiff School of Biosciences, Cardiff University, United Kingdom
    PLoS ONE 1:e17. 2006
    ....
  76. ncbi Burkholderia cepacia complex bacteria: opportunistic pathogens with important natural biology
    E Mahenthiralingam
    Cardiff School of Biosciences, Cardiff University, Main Building, Cardiff, UK
    J Appl Microbiol 104:1539-51. 2008
    ..In the last 10 years considerable progress has been made in understanding the natural biology and clinical infections caused by this fascinating group of bacteria...
  77. ncbi Burkholderia cepacia: current clinical issues, environmental controversies and ethical dilemmas
    A M Jones
    Manchester Adult Cystic Fibrosis Unit, Wythenshawe Hospital, UK
    Eur Respir J 17:295-301. 2001
    ..The article also addresses the potential conflict between medicine and agriculture on plans to reintroduce strains of Burkholderia cepacia back into the environment...
  78. ncbi Differential susceptibility of inbred mouse strains to Burkholderia thailandensis aerosol infection
    Lisa A Morici
    Department of Microbiology and Immunology, Tulane University School of Medicine, Covington, LA 70112, USA
    Microb Pathog 48:9-17. 2010
    ..These results demonstrate that B. thailandensis can be used as a surrogate for experimental laboratory investigation of melioidosis in small animal models and that TLR4 may not play a prominent role during acute pneumonic melioidosis...
  79. ncbi Burkholderia cenocepacia J2315 acyl carrier protein: a potential target for antimicrobials' development?
    Silvia A Sousa
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Avenida Rovisco Pais, Torre Sul, Piso 6, 1049 001 Lisboa, Portugal
    Microb Pathog 45:331-6. 2008
    ..pseudomallei and B. mallei...
  80. ncbi Invasion of murine respiratory epithelial cells in vivo by Burkholderia cepacia
    C H Chiu
    Department of Pediatrics, Chang Gung Children's Hospital, Taoyuan, Taiwan
    J Med Microbiol 50:594-601. 2001
    ..cepacia strains from patients with CF adhere to and then invade respiratory epithelial cells. The invasion phenotype in B. cepacia may be an important virulence factor in CF infections...
  81. ncbi Characteristics of patients with Burkholderia cepacia bacteremia
    C H Huang
    Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, ROC
    J Microbiol Immunol Infect 34:215-9. 2001
    ..In view of the fact that B. cepacia develops resistance to a wide range of antimicrobial agents, ceftazidime and/or trimethoprim/sulfamethoxazole should be the drug of choice for empiric therapy...
  82. ncbi Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy
    A Fiore
    Ente Nazionale per le Nuove Tecnologie, l'Energia e l'Ambiente, CR Casaccia, Dip. to Innovazione, Rome, Italy
    Environ Microbiol 3:137-43. 2001
    ..cepacia complex simply on the basis of genomovar status, and that the environment may serve as a reservoir for B. cepacia genomovar III infections in vulnerable humans...
  83. ncbi Lipopolysaccharide of Burkholderia cepacia complex
    Arlene D Vinion-Dubiel
    Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0734, USA
    J Endotoxin Res 9:201-13. 2003
    ..The structures of O antigen subunits and the consequent serotypes will also be discussed, with particular reference to the O antigen biosynthetic loci of two Bcc strains...
  84. ncbi Chemical and biological features of Burkholderia cepacia complex lipopolysaccharides
    Anthony De Soyza
    Transplantation and Immunobiology Group, Institute of Cellular Medicine, Newcastle University and The Freeman Hospital, Newcastle upon Tyne, UK
    Innate Immun 14:127-44. 2008
    ..In this review, we highlight important data within the field commenting on LPS/lipid A structure-to-function relationships and cytokine induction capacity of Burkholderia strains studied so far...
  85. pmc A complete lipopolysaccharide inner core oligosaccharide is required for resistance of Burkholderia cenocepacia to antimicrobial peptides and bacterial survival in vivo
    Slade A Loutet
    Department of Microbiology and Immunology, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, Canada N6A 5C1
    J Bacteriol 188:2073-80. 2006
    ..Together, these data show that the B. cenocepacia LPS inner core oligosaccharide is needed for in vitro resistance to three structurally unrelated antimicrobial peptides and for in vivo survival in a rat model of chronic lung infection...
  86. pmc Importance of the ornibactin and pyochelin siderophore transport systems in Burkholderia cenocepacia lung infections
    M B Visser
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta T2N 4N1, Canada
    Infect Immun 72:2850-7. 2004
    ..These studies suggest that the ornibactin uptake system is the most important siderophore-mediated iron transport system in B. cenocepacia lung infections...
  87. ncbi The complete structure and pro-inflammatory activity of the lipooligosaccharide of the highly epidemic and virulent gram-negative bacterium Burkholderia cenocepacia ET-12 (strain J2315)
    Alba Silipo
    Dipartimento di Chimica Organica e Biochimica, Universita di Napoli, Complesso Universitario Monte Sant Angelo, Via Cintia 4, 80126 Napoli, Italy
    Chemistry 13:3501-11. 2007
    ..The structural analysis was carried out by compositional analysis, mass spectrometry and 2D NMR spectroscopy on the intact lipooligosacchride (LOS) and its fragments, which were obtained by selective chemical degradations...
  88. pmc Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei
    Jay E Gee
    Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 41:4647-54. 2003
    ..This report provides a basis for rapidly identifying and differentiating B. pseudomallei and B. mallei by molecular methods...
  89. ncbi Interactions of Burkholderia cenocepacia and other Burkholderia cepacia complex bacteria with epithelial and phagocytic cells
    M Soledad Saldías
    Infectious Diseases Research Group, Siebens Drake Research Institute, Department of Microbiology and Immunology, University of Western Ontario, London, ON N6A 5C1, Canada
    Microbiology 155:2809-17. 2009
    ..This review summarizes recent findings concerning the interaction between Bcc bacteria and epithelial and phagocytic cells...
  90. ncbi Clinical outcome of Burkholderia cepacia complex infection in cystic fibrosis adults
    J M Courtney
    Adult Cystic Fibrosis Centre, Belfast City Hospital, Lisburn Road, Belfast BT9 7AB, North Ireland, UK
    J Cyst Fibros 3:93-8. 2004
    ..The Burkholderia cepacia complex (BCC) is one of the most important groups of organisms infecting cystic fibrosis (CF) patients. The aim of the study was to examine how infection with BCC affects clinical outcome...
  91. ncbi Mucoid and nonmucoid Burkholderia cepacia complex bacteria in cystic fibrosis infections
    James E A Zlosnik
    Centre for Understanding and Preventing Infections, 950 West 28th Avenue, Vancouver, BC, V5Z 4H4 Canada
    Am J Respir Crit Care Med 183:67-72. 2011
    ..Some BCC, but not others, elaborate copious mucoid exopolysaccharide, endowing them with a gross mucoid phenotype, the clinical significance of which has not been described...
  92. ncbi Epidemiologic investigation of Burkholderia cepacia acquisition in two pediatric intensive care units
    Chawki Loukil
    Department of Microbiology, Hopital Robert Debre, 48, boulevard Serurier, 75019 Paris, France
    Infect Control Hosp Epidemiol 24:707-10. 2003
    ..To investigate and describe an outbreak of Burkholderia cepacia in a neonatal intensive care unit (NICU) and a pediatric intensive care unit (PICU), and to report the interventions leading to the cessation of the outbreak...
  93. ncbi Investigation of Burkholderia cepacia nosocomial outbreak with high fatality in patients suffering from diseases other than cystic fibrosis
    Asem A Shehabi
    Department of Clinical Laboratories, Jordan University Hospital, Amman, Jordan
    Scand J Infect Dis 36:174-8. 2004
    ..This study demonstrates the nosocomial and high fatality of B. cepacia genomovar IIIa in Jordanian patients suffering from diseases other than cystic fibrosis...
  94. pmc Management of accidental laboratory exposure to Burkholderia pseudomallei and B. mallei
    Sharon J Peacock
    Mahidol University, Bangkok, Thailand
    Emerg Infect Dis 14:e2. 2008
    ..pseudomallei. The overall mortality rate of infected persons is 50% in northeast Thailand (35% in children) and 19% in Australia...
  95. ncbi Sustained endemicity of Burkholderia cepacia complex in a pediatric institution, associated with contaminated ultrasound gel
    Michelle Jacobson
    Hospital for Sick Children, Toronto, Ontario, Canada
    Infect Control Hosp Epidemiol 27:362-6. 2006
    ..To determine whether contaminated ultrasound gel is the source of intermittent outbreaks of nosocomial infection due to Burkholderia cepacia complex in patients without cystic fibrosis since 1992...
  96. ncbi Outbreak of Burkholderia cepacia bacteremia in immunocompetent children caused by contaminated nebulized sulbutamol in Saudi Arabia
    Sameeh S Ghazal
    Department of Infection Control and Environmental Health Administration, King Fahad Medical City, Riyadh, Saudi Arabia
    Am J Infect Control 34:394-8. 2006
    ..The Infection Control Department (ICD) in the King Fahad Medical City (KFMC) detected and investigated the outbreak to identify the source of the organism and mode of transmission...
  97. ncbi Caenorhabditis elegans killing assay as an infection model to study the role of type III secretion in Burkholderia cenocepacia
    Kevin M Markey
    J Med Microbiol 55:967-9. 2006
  98. pmc Elucidating global epidemiology of Burkholderia multivorans in cases of cystic fibrosis by multilocus sequence typing
    Adam Baldwin
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    J Clin Microbiol 46:290-5. 2008
    ..multivorans...
  99. ncbi Burkholderia cepacia complex epidemiology in persons with cystic fibrosis from Australia and New Zealand
    Timothy J Kidd
    Department of Microbiology, Pathology Queensland, The Prince Charles Hospital, Rode Road, Chermside, Brisbane, Queensland 4032, Australia
    Res Microbiol 159:194-9. 2008
    ..Although person-to-person transmission of B. cenocepacia strains has occurred in Australia, the majority of CF-related Bcc infections in Australia and New Zealand are most likely acquired from the environment...
  100. ncbi Differential interaction of bacterial species from the Burkholderia cepacia complex with human airway epithelial cells
    Jane A Moura
    Departmento de Microbiologia, Immunologia e Parasitologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil
    Microbes Infect 10:52-9. 2008
    ..Hence, our data demonstrate, for the first time, that all BCC species exhibit a similar ability to induce the expression of host immune mediators whereas they differ on their ability to adhere to, invade and kill airway epithelial cells...
  101. ncbi Evolving epidemiology of Pseudomonas aeruginosa and the Burkholderia cepacia complex in cystic fibrosis lung infection
    John R W Govan
    University of Edinburgh, Cystic Fibrosis Group, Centre for Infectious Diseases, Edinburgh, UK
    Future Microbiol 2:153-64. 2007
    ..Strict cross-infection control was prompted by early epidemiological experience of the B. cepacia complex and is essential in the management of all CF pathogens...

Research Grants5

  1. Novel Recombinant Vaccines to Protect Against Burkholderia Infections
    JOANNA GOLDBERG; Fiscal Year: 2008
    ..These constructs will be administered to mice and their efficacy tested in relevant mouse models of infection. Our long-term goal is to develop similar reagents for human use. [unreadable] [unreadable] [unreadable]..
  2. Burkholderia sp: Identification of major clonal lineages
    John LiPuma; Fiscal Year: 2004
    ..mallei and B. pseudomallei. ..
  3. Prevention/diagnosis/fungal infections/transplantation
    Barbara Alexander; Fiscal Year: 2005
    ..This approach will shorten the time from development to clinical implementation in therapeutic trials and guidelines. ..
  4. 2005 Phagocytes Gordon Conference
    Mary Dinauer; Fiscal Year: 2005
    ..The program will provide opportunities for participation of scientists at all levels and we will particularly promote the participation of junior investigators, postdoctoral trainees and "new investigators to the field". ..
  5. STRUCTURE/FUNCTION ANALYSIS OF PHAGOCYTE PROTEINS
    Mary Dinauer; Fiscal Year: 2009
    ..These studies will provide further insight into the superoxide- generating system of the phagocyte, which may lead to new approaches in modulating superoxide formation in the inflammatory response and host defense. ..