intracytoplasmic sperm injections

Summary

Summary: An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).

Top Publications

  1. ncbi Highly efficient vitrification method for cryopreservation of human oocytes
    Masashige Kuwayama
    Kato Ladies Clinic, Tokyo, Japan
    Reprod Biomed Online 11:300-8. 2005
  2. ncbi Time-dependent capability of human oocytes for activation and pronuclear formation during metaphase II arrest
    Hanna Balakier
    CReATe Program Inc Sunnybrook and Women s College Health Sciences Center, University of Toronto, Toronto, Ontario, Canada
    Hum Reprod 19:982-7. 2004
  3. ncbi Cytoplasmic lipid droplets and mitochondrial distribution in equine oocytes: Implications on oocyte maturation, fertilization and developmental competence after ICSI
    B Ambruosi
    Department of Animal Production, University of Bari, Strada Provinciale per Casamassima km 3 70010 Valenzano, Bari, Italy
    Theriogenology 71:1093-104. 2009
  4. ncbi Long-term effects of mouse intracytoplasmic sperm injection with DNA-fragmented sperm on health and behavior of adult offspring
    Raul Fernandez-Gonzalez
    Departamento de Reproduccion Animal, INIA, 28040 Madrid, Spain
    Biol Reprod 78:761-72. 2008
  5. pmc Embryo development of fresh 'versus' vitrified metaphase II oocytes after ICSI: a prospective randomized sibling-oocyte study
    Laura Rienzi
    G E N E R A Centre for Reproductive Medicine, Clinica Valle Giulia, Via De Notaris 2b, Rome, Italy
    Hum Reprod 25:66-73. 2010
  6. ncbi The outcome of ICSI of immature MI oocytes and rescued in vitro matured MII oocytes
    D Strassburger
    Assaf Harofeh Medical Center, Tel Aviv University, Zerifin, 70300, Israel
    Hum Reprod 19:1587-90. 2004
  7. ncbi Prenatal testing in ICSI pregnancies: incidence of chromosomal anomalies in 1586 karyotypes and relation to sperm parameters
    Maryse Bonduelle
    Centre for Medical Genetics and Centre for Reproductive Medicine, Dutch Speaking Brussels Free University Vrije Universiteit Brussel, Belgium
    Hum Reprod 17:2600-14. 2002
  8. ncbi Use of surgical sperm retrieval in azoospermic men: a meta-analysis
    James D M Nicopoullos
    Assisted Conception Unit, Chelsea and Westminster Hospital, London, United Kingdom
    Fertil Steril 82:691-701. 2004
  9. ncbi Predicting ongoing pregnancy chances after IVF and ICSI: a national prospective study
    A M E Lintsen
    Department of Obstetrics and Gynaecology, Radboud University Nijmegen Medical Centre, PO Box 9101, Nijmegen 6500 HB, The Netherlands
    Hum Reprod 22:2455-62. 2007
  10. ncbi Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids
    Satoshi Kishigami
    Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN Kobe, Japan
    Dev Biol 289:195-205. 2006

Research Grants

  1. Preservation of ejaculated mouse spermatozoa
    MONIKA WARD; Fiscal Year: 2006
  2. Sperm DNA damage in fertilization
    MONIKA WARD; Fiscal Year: 2006
  3. PORTABILITY OF BIOCHEMICAL MARKERS OF SPERM MATURITY
    Gabor Huszar; Fiscal Year: 2001
  4. FACTORS AFFECTING SPERM FERTILITY
    Gabor Huszar; Fiscal Year: 2004
  5. KLHL 10-mediated uniquitination during spermiogenesis
    Wei Yan; Fiscal Year: 2010
  6. Food intake and obesity in cloned mice
    Randall R Sakai; Fiscal Year: 2010
  7. Short-term course in human embryonic stem cell culture techniques
    Joseph Itskovitz Eldor; Fiscal Year: 2008
  8. DNA methylation programming to oocyte environment
    Kevin Sinclair; Fiscal Year: 2007
  9. Infrastructure for Human Embryonic Stem Cells Productio*
    Joseph Itskovitz Eldor; Fiscal Year: 2007
  10. Reproduction of Transgenic Pigs by Nuclear Transplantation
    Randall S Prather; Fiscal Year: 2010

Detail Information

Publications239 found, 100 shown here

  1. ncbi Highly efficient vitrification method for cryopreservation of human oocytes
    Masashige Kuwayama
    Kato Ladies Clinic, Tokyo, Japan
    Reprod Biomed Online 11:300-8. 2005
    ..The results suggest that vitrification using the Cryotop is the most efficient method for human oocyte cryopreservation...
  2. ncbi Time-dependent capability of human oocytes for activation and pronuclear formation during metaphase II arrest
    Hanna Balakier
    CReATe Program Inc Sunnybrook and Women s College Health Sciences Center, University of Toronto, Toronto, Ontario, Canada
    Hum Reprod 19:982-7. 2004
    ..The purpose of this study was to investigate the fertilization rate and developmental potential of human oocytes in relation to the duration of their metaphase II (MII) arrest stage following the extrusion of the first polar body (1PB)...
  3. ncbi Cytoplasmic lipid droplets and mitochondrial distribution in equine oocytes: Implications on oocyte maturation, fertilization and developmental competence after ICSI
    B Ambruosi
    Department of Animal Production, University of Bari, Strada Provinciale per Casamassima km 3 70010 Valenzano, Bari, Italy
    Theriogenology 71:1093-104. 2009
    ..However, it is not related with heterogeneous mt distribution and cannot be considered a predictive indicator for normal fertilization and embryo development...
  4. ncbi Long-term effects of mouse intracytoplasmic sperm injection with DNA-fragmented sperm on health and behavior of adult offspring
    Raul Fernandez-Gonzalez
    Departamento de Reproduccion Animal, INIA, 28040 Madrid, Spain
    Biol Reprod 78:761-72. 2008
    ..Our data indicate that use of DFS in ICSI can generate effects that only emerge during later life, such as aberrant growth, premature aging, abnormal behavior, and mesenchymal tumors...
  5. pmc Embryo development of fresh 'versus' vitrified metaphase II oocytes after ICSI: a prospective randomized sibling-oocyte study
    Laura Rienzi
    G E N E R A Centre for Reproductive Medicine, Clinica Valle Giulia, Via De Notaris 2b, Rome, Italy
    Hum Reprod 25:66-73. 2010
    ..Vitrification has been recently proposed as an effective procedure for this purpose...
  6. ncbi The outcome of ICSI of immature MI oocytes and rescued in vitro matured MII oocytes
    D Strassburger
    Assaf Harofeh Medical Center, Tel Aviv University, Zerifin, 70300, Israel
    Hum Reprod 19:1587-90. 2004
    ....
  7. ncbi Prenatal testing in ICSI pregnancies: incidence of chromosomal anomalies in 1586 karyotypes and relation to sperm parameters
    Maryse Bonduelle
    Centre for Medical Genetics and Centre for Reproductive Medicine, Dutch Speaking Brussels Free University Vrije Universiteit Brussel, Belgium
    Hum Reprod 17:2600-14. 2002
    ..Prenatal testing was offered in all pregnancies obtained after ICSI with ejaculated or non-ejaculated sperm as part of the evaluation of the safety of ICSI...
  8. ncbi Use of surgical sperm retrieval in azoospermic men: a meta-analysis
    James D M Nicopoullos
    Assisted Conception Unit, Chelsea and Westminster Hospital, London, United Kingdom
    Fertil Steril 82:691-701. 2004
    ....
  9. ncbi Predicting ongoing pregnancy chances after IVF and ICSI: a national prospective study
    A M E Lintsen
    Department of Obstetrics and Gynaecology, Radboud University Nijmegen Medical Centre, PO Box 9101, Nijmegen 6500 HB, The Netherlands
    Hum Reprod 22:2455-62. 2007
    ..We evaluated the predictive value of patient characteristics that are used in the Dutch IVF guideline and developed a model that predicts the IVF ongoing pregnancy chance within 12 months...
  10. ncbi Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids
    Satoshi Kishigami
    Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN Kobe, Japan
    Dev Biol 289:195-205. 2006
    ..These data provide insight into epigenetic errors that may be associated with the poor development of embryos generated from immature spermatozoa...
  11. ncbi Elective single-embryo transfer versus double-embryo transfer in in vitro fertilization
    Ann Thurin
    From the Department of Obstetrics and Gynecology, Institute for Health of Women and Children, Sahlgrenska University Hospital, Goteborg, Sweden
    N Engl J Med 351:2392-402. 2004
    ..The risks of premature birth and perinatal death are increased after in vitro fertilization. These risks are mainly due to the high incidence of multiple births, which relates to the number of embryos transferred...
  12. ncbi Long-term preservation of mouse spermatozoa after freeze-drying and freezing without cryoprotection
    Monika A Ward
    Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, Hawaii 96822, USA
    Biol Reprod 69:2100-8. 2003
    ..Both preservation methods are successful, but rapid freezing without cryoprotection is the preferred method for preservation of spermatozoa from mouse strains carrying unique genes and mutations...
  13. ncbi Decline in fertility of mouse sperm with abnormal chromatin during epididymal passage as revealed by ICSI
    Ryota Suganuma
    Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, HI 96822, USA
    Hum Reprod 20:3101-8. 2005
    ..These studies suggested that sperm nuclear quality declined after release from the testis, but the site where this loss of fertility occurs has not been localized...
  14. ncbi Rate of aneuploidy in miscarriages following in vitro fertilization and intracytoplasmic sperm injection
    Ruth B Lathi
    Stanford University Medical Center, Department of Obstetrics Gynecology, Division of Reproductive Endocrinology and Infertility, Stanford University Medical Center, Stanford, California 94305, USA
    Fertil Steril 81:1270-2. 2004
    ..To evaluate the incidence of aneuploidy in miscarriages after IVF and intracytoplasmic sperm injection (ICSI) procedures...
  15. pmc Aneuploidy in abortuses following IVF and ICSI
    Vitaly A Kushnir
    New Jersey Medical School, UMDNJ, Dept of Obstetrics, Gynecology and Women s Health, MSB E 506, 185 South Orange Avenue, Newark, NJ, 07101 1709, USA
    J Assist Reprod Genet 26:93-7. 2009
    ..To compare aneuploidy rates in first trimester pregnancy losses following IVF+/-ICSI...
  16. pmc Correlation between human follicular diameter and oocyte outcomes in an ICSI program
    Anusorn Triwitayakorn
    Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
    J Assist Reprod Genet 20:143-7. 2003
    ..To determine the correlation between the follicular sizes and oocyte recovery, metaphase II oocyte recovery, fertilization rate and good embryo quality from mature and immature oocytes in an intracytoplasmic sperm injection (ICSI) program...
  17. ncbi Effect of reduced oocyte aging on the outcome of rescue intracytoplasmic sperm injection
    Zsolt P Nagy
    Reproductive Biology Associates, Atlanta, Georgia 30342, USA
    Fertil Steril 85:901-6. 2006
    ..To evaluate the results of a novel protocol that allows to rescue IVF unfertilized oocytes by intracytoplasmic sperm injection (ICSI)...
  18. ncbi Release of phospholipase C zetaand [Ca2+]i oscillation-inducing activity during mammalian fertilization
    Sook Young Yoon
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003, USA
    Reproduction 134:695-704. 2007
    ....
  19. ncbi A review of ten years experience of ICSI
    P Devroey
    Centre for Reproductive Medicine, Dutch Speaking Brussels Free University Vrije Universiteit Brussel, Laarbeeklaan 101, B 1090 Brussels, Belgium
    Hum Reprod Update 10:19-28. 2004
    ..ICSI is frequently used in couples undergoing preimplantation genetic diagnosis. PGD stricto sensu as well as PGD for aneuploidy screening and for Klinefelter patients are reviewed using the ESHRE PGD Consortium data...
  20. ncbi Antimüllerian hormone predicts ovarian responsiveness, but not embryo quality or pregnancy, after in vitro fertilization or intracyoplasmic sperm injection
    Jesper M J Smeenk
    Department of Obstetrics and Gynaecology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Fertil Steril 87:223-6. 2007
    ..In a multivariate regression analysis, AMH was found to be predictive of the number of oocytes and the number of embryos, but not of embryo quality or the chance of a pregnancy, after IVF/ICSI...
  21. ncbi Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-analysis
    Armand Zini
    Department of Surgery, Division of Urology, St Mary s Hospital Center, Mary s Hospital, Montreal, Quebec, Canada
    Hum Reprod 23:2663-8. 2008
    ..The aim of this study was to examine the influence of sperm DNA damage on the risk of spontaneous pregnancy loss after IVF and ICSI...
  22. ncbi Possible predictive factors for ICSI? Molecular biology techniques in combination with therapeutic testicular biopsies
    K Steger
    Institute of Veterinary Anatomy, Histology and Embryology, Giessen, Germany
    Andrologia 35:200-8. 2003
    ..In future, both the outstanding role of the haploid spermatids and the involvement of molecular biological techniques will improve the role of therapeutic testicular biopsies...
  23. ncbi Egg activation events are regulated by the duration of a sustained [Ca2+]cyt signal in the mouse
    Jean Pierre Ozil
    Unité de Biologie du Développement et Reproduction, INRA, 78352 Jouy en Josas Cedex, France
    Dev Biol 282:39-54. 2005
    ..Thus, the total Ca2+ signal input appears to be an important regulatory parameter that ensures completion of fertilization-associated events and oocytes have a surprising degree of tolerance for a prolonged change in [Ca2+]cyt...
  24. ncbi [Ten years of results of in-vitro fertilisation in the Netherlands 1996-2005]
    J A M Kremer
    Universitair Medisch Centrum St Radboud, Afd Gynaecologie, Nijmegen
    Ned Tijdschr Geneeskd 152:146-52. 2008
    ..To present the numbers and results of Dutch IVF treatment from 1996-2005 and to describe trends and differences between centres...
  25. ncbi Sperm defect severity rather than sperm Source is associated with lower fertilization rates after intracytoplasmic sperm injection
    Sidney Verza
    Androfert, Center for Male Reproduction, Campinas, Sao Paulo, Brazil
    Int Braz J Urol 34:49-56. 2008
    ..To evaluate the impact of sperm defect severity and the type of azoospermia on the outcomes of intracytoplasmic sperm injection (ICSI)...
  26. ncbi A prospective, randomized, controlled trial comparing highly purified hMG with recombinant FSH in women undergoing ICSI: ovarian response and clinical outcomes
    Z Kilani
    Farah Hospital, Amman, Jordan and Reproductive Endocrinology Center, University of Bologna, Via Massarenti 13 40138, Italy
    Hum Reprod 18:1194-9. 2003
    ....
  27. ncbi A family history of twinning in relation to multiple implantation
    M J Lambers
    Department of Obstetrics, Gynecology and Reproductive Medicine, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam, The Netherlands
    Hum Reprod 23:889-93. 2008
    ..If multiple implantation is not hereditary, the incidences of twins in families of patients with single and multiple implantation after IVF/ICSI with double embryo transfer (DET) should be comparable...
  28. ncbi Evidence of a high proportion of premature unbalanced separation of sister chromatids in the first polar bodies of women of advanced age
    F Vialard
    Department of Reproductive Biology, Cytogenetics, Gynaecology and Obstetrics, CHI Poissy Saint Germain, Poissy, France
    Hum Reprod 21:1172-8. 2006
    ..Previous studies of unfertilized oocytes argue for a major role of PSSC in the aetiology of aneuploidy for women of advanced age, but in vitro ageing of the oocytes could influence the results...
  29. pmc Severe oligoasthenoteratozoospermias, secretory and obstructive azoospermias: motility as a criterion of sperm viability
    I Molina
    Human Assisted Reproduction Unit, La Fe University Hospital of Valencia, Valencia, Spain
    J Assist Reprod Genet 24:125-9. 2007
    ..Comparison of pregnancy rates in cases of Secretory Azoospermias (SA), Obstructive Azoospermias (OA) and severe Oligoasthenoteratozoospermias (OATZ). Evaluation of sperm motility as a quality criterion...
  30. ncbi Ejaculated and epididymal mouse spermatozoa are different in their susceptibility to nuclease-dependent DNA damage and in their nuclease activity
    Yasuhiro Yamauchi
    Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, Hawaii 96822, USA
    Biol Reprod 77:636-47. 2007
    ..This nuclease activity was detected both in the sperm-free fraction of preparations and isolated sperm...
  31. ncbi Zygote transfer on day 1 versus cleavage stage embryo transfer on day 3: a prospective randomized trial
    Kamal Jaroudi
    King Faisal Specialist Hospital and Research Center, Department of Obstetrics and Gynecology, Laboratory Medicine, Riyadh, Saudi Arabia
    Hum Reprod 19:645-8. 2004
    ..The objective of this study was to investigate whether transferring zygotes on day 1 would result in similar pregnancy rates compared to transferring cleavage stage embryos on day 3 in a prospective randomized trial...
  32. ncbi Apoptosis in human unfertilized oocytes after intracytoplasmic sperm injection
    Liana Bosco
    Dipartimento Materno Infantile, University of Palermo, Palermo, Italy
    Fertil Steril 84:1417-23. 2005
    ..To investigate the presence of programmed cell death in unfertilized oocytes after intracytoplasmic sperm injection (ICSI), assuming that previous apoptotic events could be correlated with the fertilization failure...
  33. ncbi Impact of overweight and underweight on assisted reproduction treatment
    Peter Fedorcsak
    Department of Obstetrics and Gynaecology, Rikshospitalet University Hospital, Oslo, Norway
    Hum Reprod 19:2523-8. 2004
    ..Underweight and overweight may affect reproduction and interfere with treatment of infertility. The purpose of this report is to describe the independent effect of body weight on treatment with IVF and ICSI...
  34. ncbi Neonatal outcome in a Danish national cohort of 8602 children born after in vitro fertilization or intracytoplasmic sperm injection: the role of twin pregnancy
    Anja Pinborg
    The Fertility Clinic, University of Copenhagen, Rigshospitalet, Denmark
    Acta Obstet Gynecol Scand 83:1071-8. 2004
    ..In Denmark, 4% of all infants are born after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and 40% of these children are twins...
  35. ncbi A prospective study of predictive factors of ovarian response in 'standard' IVF/ICSI patients treated with recombinant FSH. A suggestion for a recombinant FSH dosage normogram
    B Popovic-Todorovic
    The Fertility Clinic, Rigshospitalet, Copenhagen University Hospital, Blegdamsvej 9, 2100 Copenhagen, Denmark
    Hum Reprod 18:781-7. 2003
    ..The aim was to identify independent predictors of ovarian response to recombinant (r)FSH through a multiple regression analysis...
  36. ncbi Early embryo cleavage is a strong indicator of embryo quality in human IVF
    K Lundin
    Department of Obstetrics and Gynecology, Goteborg University, Sahlgrenska University Hospital, Gothenburg, Sweden
    Hum Reprod 16:2652-7. 2001
    ..It has been shown that human embryos which cleave early, i.e. complete the first mitotic division within 25-27 h post insemination, provide higher pregnancy and implantation rates...
  37. ncbi Factors influencing the outcome of ICSI in patients with obstructive and non-obstructive azoospermia: a comparative study
    S Friedler
    Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center, Zerifin 70300, Israel
    Hum Reprod 17:3114-21. 2002
    ..Factors influencing success of sperm retrieval in azoospermic patients and outcome of ICSI were evaluated...
  38. ncbi One versus two embryo transfer after IVF and ICSI: a randomized study
    H Martikainen
    Department of Obstetrics and Gynecology, Oulu University Central Hospital, Oulu, Finland
    Hum Reprod 16:1900-3. 2001
    ..The main reason for adverse treatment outcome in assisted reproduction is the high rate of multiple pregnancies. The only strategy to avoid dizygotic twins is to transfer one embryo at a time...
  39. ncbi Age- and substrain-dependent sperm abnormalities in BALB/c mice and functional assessment of abnormal sperm by ICSI
    Hiroshi Ohta
    Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Hum Reprod 24:775-81. 2009
    ..Although the BALB/c male may be a useful model of human male infertility, it remains unclear whether the sperm abnormality rate (SAR) is affected by age or the BALB/c substrain...
  40. ncbi Embryo quality and implantation rate in two different culture media: ISM1 versus Universal IVF Medium
    Susanna Xella
    Mother Infant Department, Institute of Obstetrics and Gynaecology, University of Modena and Reggio Emilia, Modena, Italy
    Fertil Steril 93:1859-63. 2010
    ....
  41. ncbi Human menopausal gonadotropin versus recombinant follicle-stimulating hormone in normogonadotropic women down-regulated with a gonadotropin-releasing hormone agonist who were undergoing in vitro fertilization and intracytoplasmic sperm injection: a prospe
    L G Westergaard
    Fertility Clinic, Department of Obstetrics and Gynecology, Odense University Hospital, Odense, Denmark
    Fertil Steril 76:543-9. 2001
    ..To evaluate clinical and endocrinological effects of intranasal (IN) vs. subcutaneous (SC) GnRH-a for pituitary down-regulation combined with hMG vs. rFSH...
  42. ncbi The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development
    I D Morris
    School of Biological Sciences, University of Manchester, Manchester, UK
    Hum Reprod 17:990-8. 2002
    ..ICSI, by bypassing sperm selection mechanisms, increases the risk of transmitting damaged DNA and the significance of this requires investigation...
  43. ncbi Developmental competence in vivo and in vitro of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection with fresh or frozen-thawed spermatozoa
    Y H Choi
    Departments of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843 4466, USA
    Reproduction 123:455-65. 2002
    ..2 medium results in a similar cleavage rate but reduced number of cells compared with in vivo culture within the oviduct. Bovine oocytes may be useful as models for assessing sperm function in horses...
  44. ncbi Prevention of severe ovarian hyperstimulation syndrome in IVF with or without ICSI and embryo transfer: a modified 'coasting' strategy based on ultrasound for identification of high-risk patients
    T Al-Shawaf
    Fertility Centre, St Bartholomew s Hospital, West Smithfield, London, EC1A 7BE, UK
    Hum Reprod 16:24-30. 2001
    ..Identification of patients at risk by ultrasound reduces the number of serum oestradiol measurements and thus inconvenience to patients as well as costs and workload...
  45. ncbi Vitrification of oocytes produces high pregnancy rates when carried out in fertile women
    Thomas J Kim
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
    Fertil Steril 93:467-74. 2010
    ..To determine the efficiency of our vitrification technique when applied in young fertile women...
  46. ncbi Fertilization, pregnancy and embryo implantation rates after ICSI in cases of obstructive and non-obstructive azoospermia
    I De Croo
    Infertility Centre, Department of Obstetrics and Gynaecolog, University Hospital of Ghent, Belgium
    Hum Reprod 15:1383-8. 2000
    ....
  47. ncbi Meiotic competence of equine oocytes and pronucleus formation after intracytoplasmic sperm injection (ICSI) as related to granulosa cell apoptosis
    Maria Elena Dell'Aquila
    Department of Animal Production Section of Reproductive Biology and Veterinary Obstetrics, University of Bari, 70010 Valenzano, Bari, Italy
    Biol Reprod 68:2065-72. 2003
    ..These results provide selection criteria for horse oocytes used in assisted reproductive techniques so that embryo production may be maximized...
  48. pmc Efficiency of using frozen-thawed testicular sperm for multiple intracytoplasmic sperm injections
    N Fukunaga
    Ladies Clinic Kyono, Furukawa, Miyagi, Japan
    J Assist Reprod Genet 18:634-7. 2001
    ..To compare fertilization and pregnancy rates of fresh and frozen-thawed testicular sperm injections (TESE-ICSI)...
  49. ncbi Chromatin configuration within the germinal vesicle of horse oocytes: changes post mortem and relationship to meiotic and developmental competence
    K Hinrichs
    Department of Veterinary, College of Veterinary Medicine, Texas A and M University, Texas 77843 4466, USA
    Biol Reprod 72:1142-50. 2005
    ..Time of oocyte recovery and duration of maturation significantly affect the rate of blastocyst development. Oocytes with Cp and Ex cumuli have similar developmental competence to the blastocyst stage...
  50. ncbi Androgen priming using aromatase inhibitor and hCG during early-follicular-phase GnRH antagonist down-regulation in modified antagonist protocols
    K Lossl
    The Fertility Clinic 4071, Laboratory of Reproductive Biology 5712, Copenhagen University Hospital, Rigshospitalet, Denmark
    Hum Reprod 21:2593-600. 2006
    ..The aim of this study was to increase the intraovarian androgen level using aromatase inhibitors and hCG before controlled ovarian stimulation (COH) and to test this concept clinically...
  51. ncbi The optimal time for intracytoplasmic sperm injection in the human is from 37 to 41 hours after administration of human chorionic gonadotropin
    Dmitri Dozortsev
    Clinica e Centro de Pesquisa em Reproducao Humana Roger Abdelmassih, Sao Paulo, Brazil
    Fertil Steril 82:1492-6. 2004
    ..However, the highest implantation rate, 24%, was achieved when ICSI was performed 37-41 hours after hCG administration...
  52. ncbi Spontaneous embryonic loss rates in twin and singleton pregnancies after transfer of top- versus intermediate-quality embryos
    Giovanni B La Sala
    Department of Obstetrics and Gynecology, Arcispedale Santa Maria Nuova, Reggio Emilia, Italy
    Fertil Steril 84:1602-5. 2005
    ..To determine whether embryo quality is associated with early spontaneous loss rates in twin and singleton pregnancies after IVF/intracytoplasmic sperm injection (ICSI)...
  53. ncbi Comparison of intracytoplasmic sperm injection for inbred and hybrid mice
    Y Kawase
    Pharmaceutical Technology Laboratory, Chugai Pharmaceutical Co, Ltd, Shizuoka, Japan
    Mol Reprod Dev 60:74-8. 2001
    ..Reciprocal crosses of C57BL/6 and B6C3F1 gametes revealed that the low rate of post-implantation development of C57BL/6 embryos was due to oocyte factor(s), rather than the sperm factor...
  54. ncbi Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection
    Akie Shinmen
    RIKEN BioResource Center, Tsukuba, Ibaraki, Japan
    Mol Reprod Dev 74:1081-8. 2007
    ..Thus, ICSI using cryopreserved spermatozoa allows the efficient and immediate production of a number of F1 hybrid mice and ES cell lines, which can be used for polymorphic analysis of mouse genetics...
  55. ncbi The effect of intracytoplasmic sperm injection and semen parameters on blastocyst development in vitro
    J E Miller
    North Hudson IVF Center, 385 Sylvan Avenue, Englewood Cliffs, NJ 07632, USA
    Hum Reprod 16:918-24. 2001
    ..05). From these data, we conclude that paternal factors and/or performing ICSI in cases of severe male factor infertility may have a detrimental effect on blastocyst development and their quality...
  56. ncbi Successful ongoing pregnancies after vitrification of oocytes
    Elkin Lucena
    Centro Colombiano de Fertilid y Esterilidad CECOLFES LTDA, Bogota, Colombia
    Fertil Steril 85:108-11. 2006
    ..To demonstrate the efficiency of vitrifying mature human oocytes for different clinical indications...
  57. ncbi Intracytoplasmic sperm injection in the bovine induces abnormal [Ca2+]i responses and oocyte activation
    Christopher Malcuit
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
    Reprod Fertil Dev 18:39-51. 2006
    ..However, either the release and/or activation of the sperm factor are compromised after ICSI, leading to the delivery of a defective Ca2+ stimulus, which results in premature termination of embryo development...
  58. ncbi Influence of oocyte collection technique on initial chromatin configuration, meiotic competence, and male pronucleus formation after intracytoplasmic sperm injection (ICSI) of equine oocytes
    M E Dell'Aquila
    Department of Animal Production Section of Reproduction, University of Bari, Valenzano, Bari, Italy
    Mol Reprod Dev 60:79-88. 2001
    ..6/94, 6%, respectively; P <0.05). These results demonstrate that the collection method affects the population of recovered oocytes and may contribute to differences in results observed among laboratories working with horse oocytes...
  59. ncbi Impact of oocyte cryopreservation on embryo development
    M Cristina Magli
    Reproductive Medicine Unit, Società Italiana Studi Medicina della Riproduzione, Bologna, Italy
    Fertil Steril 93:510-6. 2010
    ..To verify whether the morphologic evaluation of zygotes and embryos derived from thawed oocytes could provide some relevant information regarding their developmental performance...
  60. ncbi Pregnancies and births after oocyte cryopreservation
    Andrea Borini
    Tecnobios Procreazione, Via Dante 15, 40125 Bologna, Italy
    Fertil Steril 82:601-5. 2004
    ..To determine the potential of oocyte cryopreservation techniques...
  61. ncbi The effects of deletions of the mouse Y chromosome long arm on sperm function--intracytoplasmic sperm injection (ICSI)-based analysis
    Monika A Ward
    Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    Biol Reprod 74:652-8. 2006
    ..Thus, a normal complement of the Ssty and/or Sly gene families on mouse Yq appears necessary for normal sperm function...
  62. ncbi Blastocyst transfer following intracytoplasmic injection of ejaculated, epididymal or testicular spermatozoa
    B Balaban
    Assisted Reproduction Unit, American Hospital of Istanbul, Turkey
    Hum Reprod 16:125-129. 2001
    ..Spermatozoa from non-obstructive azoospermic subjects, when utilized for ICSI, result in embryos that progress to the blastocyst stage at a lower and slower rate and implant less efficiently...
  63. ncbi Cytogenetic analysis of early nonviable pregnancies after assisted reproduction treatment
    M Carmen Martinez
    Instituto Valenciano de Infertilidad in Murcia, Murcia, Spain
    Fertil Steril 93:289-92. 2010
    ....
  64. ncbi Basal and stimulation day 5 anti-Mullerian hormone serum concentrations as predictors of ovarian response and pregnancy in assisted reproductive technology cycles stimulated with gonadotropin-releasing hormone agonist--gonadotropin treatment
    Joana Peñarrubia
    Institut Clinic of Gynecology, Obstetrics and Neonatology, Faculty of Medicine University of Barcelona, Hospital Clínic Institut d Investigacions Biomèdiques August Pi i Sunyer IDIBAPS, Barcelona, Spain
    Hum Reprod 20:915-22. 2005
    ....
  65. ncbi Mouse zygotes with one diploid pronucleus formed as a result of ICSI can develop normally beyond birth
    Anna Krukowska
    Department of Embryology, Institute of Zoology, Faculty of Biology, Warsaw University, Warsaw, Poland
    Mol Reprod Dev 72:346-53. 2005
    ....
  66. ncbi Influence of co-culture during maturation on the developmental potential of equine oocytes fertilized by intracytoplasmic sperm injection (ICSI)
    X Li
    Thoroughbred Breeders' Association Equine Fertility Unit, Mertoun Paddocks, Woodditton Road, Newmarket CB8 9BH, UK
    Reproduction 121:925-32. 2001
    ..These results demonstrate a beneficial influence of co-culture with either oviduct epithelial cells or fetal fibroblasts for maturation of oocytes in vitro...
  67. ncbi Number of embryos for transfer after IVF and ICSI: a Cochrane review
    Zabeena Pandian
    Department of Obstetrics and Gynaecology, Aberdeen Maternity Hospital, Aberdeen, UK
    Hum Reprod 20:2681-7. 2005
    ..Single embryo transfer can minimize twin pregnancies but could also lower live birth rates. Our aim was to perform a systematic review of randomized trials to determine the effectiveness of single versus double embryo transfer...
  68. ncbi Blastocyst formation in in vitro fertilization versus intracytoplasmic sperm injection cycles: influence of the fertilization procedure
    Lisbet Van Landuyt
    Center for Reproductive Medicine, University Hospital, Dutch Speaking Brussels Free University, Vrije Universiteit Brussel VUB, Brussels, Belgium
    Fertil Steril 83:1397-403. 2005
    ..To compare the quality of early cleaving embryos and blastocysts obtained by IVF or intracytoplasmic sperm injection (ICSI)...
  69. ncbi Algorithm to predict assisted reproductive technology pregnancy outcome reveals minimal embryo synergy
    Sergey P Torsky
    Baylor Assisted Reproductive Technology Program, Baylor College of Medicine, Houston, Texas
    Fertil Steril 83:782-4. 2005
    ..The algorithm was validated by comparing the calculated pregnancy probability and multiple pregnancy probability with the actual outcome of 281 separate IVF/ICSI cases. Finally, an estimation of embryo synergy was calculated...
  70. pmc Live offspring from mice lacking the Y chromosome long arm gene complement
    Yasuhiro Yamauchi
    Institute for Biogenesis Research, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    Biol Reprod 81:353-61. 2009
    ..The present work also led to development of a new mouse model lacking NPYq gene complement that will facilitate future studies of Y-encoded gene function...
  71. ncbi Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture, cryopreservation and somatic cell nuclear transfer
    C Galli
    Laboratorio di Tecnologie della Riproduzione, Istituto Sperimentale Italiano Lazzaro Spallanzani, CIZ s r l, Via Porcellasco 7f, 26100 Cremona, Italy
    Anim Reprod Sci 98:39-55. 2007
    ..These percentages are reduced to 11 and 23%, respectively, for cloned embryos. In conclusion, extensive evidence exists that in vitro matured equine oocytes can efficiently develop into viable embryos and offspring...
  72. ncbi Cryopreservation of supernumerary oocytes in IVF/ICSI cycles
    P E Levi Setti
    UO di Medicina della Riproduzione, IRCCS Istituto Clinico Humanitas, Rozzano Milano, Italy
    Hum Reprod 21:370-5. 2006
    ....
  73. ncbi Intracytoplasmic injection of spermatozoa and spermatogenic cells: its biology and applications in humans and animals
    Ryuzo Yanagimachi
    Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, Hawaii 96822, USA
    Reprod Biomed Online 10:247-88. 2005
    ..Whether human ROSI and assisted fertilization involving younger spermatogenic cells are medically safe must be the subject of further investigations...
  74. ncbi Comparison of GnRH agonists and antagonists in normoresponder IVF/ICSI in Turkish female patients
    Ozlem Moraloglu
    Zekai Tahir Burak Women s Health and Education Hospital, Ankara, Turkey
    Adv Ther 25:266-73. 2008
    ....
  75. ncbi Is intracytoplasmic sperm injection safe?
    Matthew G Retzloff
    Department of Obstetrics and Gynecology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Fertil Steril 80:851-9. 2003
    ..To review the pregnancy and birth outcomes of patients undergoing intracytoplasmic sperm injection (ICSI)...
  76. ncbi Fertilization of oocytes and birth of normal pups following intracytoplasmic injection with spermatids in mastomys (Praomys coucha)
    Narumi Ogonuki
    BioResource Center, RIKEN, Tsukuba, Ibaraki 305 0074, Japan
    Biol Reprod 68:1821-7. 2003
    ....
  77. ncbi Activation regimens for full-term development of rabbit oocytes injected with round spermatids
    Masumi Hirabayashi
    Section of Mammalian Transgenesis, Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi, Japan
    Mol Reprod Dev 76:573-9. 2009
    ..In conclusion, rabbit ROSI oocytes were capable of developing into full-term when the oocytes were activated with a combined treatment of ionomycin and CHX/DMAP...
  78. ncbi Embryo technologies in the horse
    E L Squires
    Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO 80523, USA
    Theriogenology 59:151-70. 2003
    ..Production of a cloned horse appears to be likely, as several cloned pregnancies have recently been produced...
  79. ncbi Predictive value of testicular histology in secretory azoospermic subgroups and clinical outcome after microinjection of fresh and frozen-thawed sperm and spermatids
    M Sousa
    Department of Medical Genetics, Faculty of Medicine, University of Porto, Portugal
    Hum Reprod 17:1800-10. 2002
    ....
  80. ncbi Presence, but not type or degree of extension, of a cytoplasmic halo has a significant influence on preimplantation development and implantation behaviour
    T Ebner
    Women s General Hospital, IVF Unit, Lederergasse 47, A 4010 Linz, Upper Austria, Austria
    Hum Reprod 18:2406-12. 2003
    ..Since there is considerable disagreement in grading cytoplasmic haloes, this prospective study was set up to evaluate if certain subtypes of haloes are related to further development...
  81. ncbi Effects of exogenous LH administration during ovarian stimulation of pituitary down-regulated young oocyte donors on oocyte yield and developmental competence
    Jan Tesarik
    MAR and Gen, Molecular Assisted Reproduction and Genetics, Gracia 36, 18002 Granada
    Hum Reprod 17:3129-37. 2002
    ..The role of exogenous LH supplementation in ovarian stimulation is a matter of debate. Here we evaluate the impact of exogenous LH on oocyte yield and developmental competence in an oocyte donation programme...
  82. ncbi Comparison of ICSI and conventional IVF in patients with increased oocyte immaturity
    Tyl H Taylor
    Main Line Fertility, 130 S Bryn Mawr Avenue, Suite 1000, D Wing, Bryn Mawr, PA 19010, USA
    Reprod Biomed Online 17:46-52. 2008
    ..Furthermore, IVF provided more embryos available for transfer or cryopreservation when compared with ICSI, thereby optimizing the patient's cycle...
  83. ncbi Significance of metaphase II human oocyte morphology on ICSI outcome
    Laura Rienzi
    Reproductive Medicine, European Hospital, Rome, Italy
    Fertil Steril 90:1692-700. 2008
    ..The identification of oocyte quality markers is particularly important when a low number of oocytes can be used for IVF...
  84. ncbi Intracytoplasmic sperm injection is more efficient than in vitro fertilization for generating mouse embryos from cryopreserved spermatozoa
    Monika A Szczygiel
    Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu 96822, USA
    Biol Reprod 67:1278-84. 2002
    ..More important, ICSI is especially valuable for strains where IVF with fresh spermatozoa produces few or no embryos...
  85. ncbi The developmental ability of vitrified oocytes from different mouse strains assessed by parthenogenetic activation and intracytoplasmic sperm injection
    Keisuke Endoh
    RIKEN BioResource Center, Tsukuba, Japan
    J Reprod Dev 53:1199-206. 2007
    ..Moreover, the oocytes of these four major inbred strains of mice can be cryopreserved safely for production of offspring...
  86. ncbi Freezing within 2 h from oocyte retrieval increases the efficiency of human oocyte cryopreservation when using a slow freezing/rapid thawing protocol with high sucrose concentration
    L Parmegiani
    Reproductive Medicine Unit, GynePro Medical Centers, Via Tranquillo Cremona 8, 40137 Bologna, Italy
    Hum Reprod 23:1771-7. 2008
    ..The aim of the present study is to establish the ideal time, after oocyte retrieval, for human oocyte cryopreservation via slow freezing/rapid thawing protocol with 0.3 M sucrose concentration in cryoprotectant solution (SF/RT 0.3 M)...
  87. ncbi Attitudes of IVF/ICSI-twin mothers towards twins and single embryo transfer
    Anja Pinborg
    The Fertility Clinic, University of Copenhagen, Rigshospitalet, Blegdamsvej 9, DK 2100 Copenhagen Ø, Denmark
    Hum Reprod 18:621-7. 2003
    ..Our aim was to study attitudes towards twins and single embryo transfer (SET) among IVF/ICSI-twin mothers with 3 to 4-year old children...
  88. ncbi Blastocyst formation rates in vivo and in vitro of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection
    Y H Choi
    Department of Veterinary Physiology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843, USA
    Biol Reprod 70:1231-8. 2004
    ..While we identified an in vitro culture system that provided repeatable blastocyst development without coculture, this yielded only half the rate of development achieved in vivo...
  89. ncbi Outcome from consecutive assisted reproduction cycles in patients treated with recombinant follitropin alfa filled-by-bioassay and those treated with recombinant follitropin alfa filled-by-mass
    Juan Balasch
    Institut Clinic of Gynecology, Obstetrics and Neonatology, Faculty of Medicine University of Barcelona, Hospital Clinic, Institut d Investigacions Biomediques August Pi i Sunyer IDIBAPS, Barcelona, Spain
    Reprod Biomed Online 8:408-13. 2004
    ..It is concluded that the new formulation of FSH-mass is more effective than the standard FSH-bio in terms of embryo quality, implantation rates, and number of days of stimulation...
  90. ncbi Intracytoplasmic sperm injection with testicular spermatozoa is less successful in men with nonobstructive azoospermia than in men with obstructive azoospermia
    Valerie Vernaeve
    Center for Reproductive Medicine, University Hospital, Dutch Speaking Brussels Free University Vrije Universiteit Brussel, Belgium
    Fertil Steril 79:529-33. 2003
    ..To assess the efficiency of intracytoplasmic sperm injection (ICSI) using testicular spermatozoa in cases of nonobstructive azoospermia...
  91. ncbi Reduced oocyte activation and first cleavage rate after ICSI with spermatozoa from a sterile mouse chromosome mutant
    E B Baart
    Laboratory of Genetics, Wageningen Institute of Animal Sciences, Wageningen University, 6701BH Wageningen, The Netherlands
    Hum Reprod 19:1140-7. 2004
    ..Male mice, heterozygous for two semi-identical reciprocal translocations T(1;13)70H and T(1;13)1Wa are usually sterile. We have investigated this oligoasthenoteratozoospermic mouse model using ICSI...
  92. ncbi Microinsemination with first-wave round spermatids from immature male mice
    Hiromi Miki
    Institute of Physical and Chemical Research RIKEN, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    J Reprod Dev 50:131-7. 2004
    ..The use of male germ cells from immature animals may save time in the production of inbred/congenic strains and rescue male-factor infertility of early onset...
  93. ncbi A comparative study of the effect of ovarian stimulation protocols with different gonadotropin preparations on the biological and clinical parameters of the outcome of intracytoplasmic sperm injection
    P Drakakis
    IVF Unit, Alexandra Maternity Hospital, 1st Dept of OB GYN, Athens University Medical School, Athens, Greece
    Clin Exp Obstet Gynecol 29:286-9. 2002
    ..This is very helpful for the physician to know since a gonadotropin with a lower cost can be employed and, in addition, shortage of some preparations of gonadotropins occurs frequently...
  94. pmc Intracytoplasmic sperm injection with testicular spermatozoa in men with azoospermia
    M L Windt
    Department of Obstetrics and Gynaecology, University of Stellenbosch, Tygerberg Hospital, South Africa
    J Assist Reprod Genet 19:53-9. 2002
    ..The aim of the study was to gain an insight into the optimal management of the infertile couple with the husband suffering from azoospermia...
  95. ncbi [Prospective evaluation of elective single-embryo transfer versus double-embryo transfer following in vitro fertilization: a two-year French hospital experience]
    L Leniaud
    Service d Histologie Embryologie Cytogénétique, Laboratoire de Biologie de la Reproduction, CHU Jean Verdier, Assistance Publique Hopitaux de Paris AP HP, Avenue du 14 Juillet, 93140 Bondy, France
    Gynecol Obstet Fertil 36:159-65. 2008
    ..This prospective non randomized study aimed to assess the ability of an elective single-embryo transfer (eSET) policy to reduce the twin pregnancy rate, compared to a double embryo transfer (DET) approach...
  96. ncbi In vitro production and transfer of cat embryos in the 21st century
    C E Pope
    Audubon Nature Institute Center for Research of Endangered Species, New Orleans, LA 70131, USA
    Theriogenology 66:59-71. 2006
    ..Furthermore, extrapolating the domestic biotechniques to non-domestic felids has produced encouraging results in some species...
  97. ncbi Normal developmental competence to the blastocyst stage is preserved in rabbit ovarian tissue following cryopreservation and autografting to the mesometrium
    Lan Chao
    Infertility Center, Qilu Hospital, Shandong University, Jinan 250012, China
    Reprod Fertil Dev 20:466-73. 2008
    ..In conclusion, the mesometrium is a promising site for ovarian autografts in the rabbit. Oocytes retrieved from mesometrial grafts can develop to the blastocyst stage...
  98. ncbi Conventional in vitro fertilization versus intracytoplasmic sperm injection in patients with borderline semen: a randomized study using sibling oocytes
    Lucette van der Westerlaken
    Department of Reproductive Medicine, Leiden University Medical Center, Leiden, The Netherlands
    Fertil Steril 85:395-400. 2006
    ..To determine whether patients with borderline semen should be treated with conventional IVF or intracytoplasmic sperm injection (ICSI)...
  99. ncbi Assessing centrosomal function of infertile males using heterologous ICSI
    Tomoko Yoshimoto-Kakoi
    Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Miyagi, Japan
    Syst Biol Reprod Med 54:135-42. 2008
    ..These results suggested that centrosomal function is essential for pregnancy and embryonic development. The method described using bovine eggs is suitable to assay human centrosome function and predict pregnancy after ART...
  100. ncbi [In vitro maturation and fertilization of unstimulated immature oocyte for the treatment of infertile women]
    Jun Zhao Zhao
    Reproductive Medicine Center, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China
    Zhonghua Fu Chan Ke Za Zhi 41:173-6. 2006
    ..To evaluate the efficacy of in vitro maturation (IVM) and fertilization of unstimulated immature oocytes for the treatment of infertile women...
  101. ncbi Effect of aspiration vacuum on the developmental competence of immature human oocytes retrieved using a 20-gauge needle
    Shu Hashimoto
    IVF Namba Clinic, 1 17 28 Minamihorie, Nishi ku, Osaka, 550 0015 Japan
    Reprod Biomed Online 14:444-9. 2007
    ..01) than that in 300 mmHg (4.3%). The data indicate that lower pressure of vacuum aspiration with a 20-gauge needle improves the developmental competence of immature oocytes following IVM, IVF and embryo transfer...

Research Grants16

  1. Preservation of ejaculated mouse spermatozoa
    MONIKA WARD; Fiscal Year: 2006
    ..Our expectations are that the results of this application will allow us to recommend ICSI with preserved ejaculated spermatozoa as a simple and efficient method for preservation of valuable mouse genomes. ..
  2. Sperm DNA damage in fertilization
    MONIKA WARD; Fiscal Year: 2006
    ....
  3. PORTABILITY OF BIOCHEMICAL MARKERS OF SPERM MATURITY
    Gabor Huszar; Fiscal Year: 2001
    ..We will identify and make accessible the most effective and practical methods, in order to simplify the monitoring of reproductive toxicity. ..
  4. FACTORS AFFECTING SPERM FERTILITY
    Gabor Huszar; Fiscal Year: 2004
    ..non-bound cells; and Aim #4 - extension of the above to testicular biopsies and analysis of spherical spermatids. ..
  5. KLHL 10-mediated uniquitination during spermiogenesis
    Wei Yan; Fiscal Year: 2010
    ..On the other hand, KLHL10 and its substrate proteins may be good targets for future non-hormonal male contraceptives. ..
  6. Food intake and obesity in cloned mice
    Randall R Sakai; Fiscal Year: 2010
    ..In addition, the behavioral, physiological, and neurochemical data obtained will enhance our understanding of the mechanisms of obesity, a serious and growing chronic public health issue worldwide. ..
  7. Short-term course in human embryonic stem cell culture techniques
    Joseph Itskovitz Eldor; Fiscal Year: 2008
    ..Thus, this guiding program will further encourage the distribution and research based on hESC. [unreadable] [unreadable] [unreadable]..
  8. DNA methylation programming to oocyte environment
    Kevin Sinclair; Fiscal Year: 2007
    ..abstract_text> ..
  9. Infrastructure for Human Embryonic Stem Cells Productio*
    Joseph Itskovitz Eldor; Fiscal Year: 2007
    ..These improvements will constitute the ability to grow and propagate hESCs in large quantities in conditions that promote both research and clinical utilization of these cells. ..
  10. Reproduction of Transgenic Pigs by Nuclear Transplantation
    Randall S Prather; Fiscal Year: 2010
    ..The third aim is to determine the methylation profile and transcriptional profile of two different types of stem cells as compared to fetal-derived fibroblast-like cells. ..
  11. Role of integrins and activin in granulosa cell growth
    Kutluk Oktay; Fiscal Year: 2007
    ..Cell proliferation and survival will be determined before and after transfection with the dominant-negative mutants. ..
  12. GENOMIC & NONGENOMIC EFFECTS OF STEROIDS ON SALT INTAKE
    Randall Sakai; Fiscal Year: 2004
    ....
  13. Angiogenesis Induction by Vasculogenic Progenitor Cells
    Joseph Itskovitz Eldor; Fiscal Year: 2006
    ..We expect that this research will establish the foundation for an effective therapeutic modality using hES cells for some of the most leading causes of morbidity and mortality in the world. ..
  14. A NOVEL APPROACH TO GERM-LIINE PRESERVATION IN MACAQUES
    Stuart Meyers; Fiscal Year: 2004
    ..Dobrinski and experiments involving germ cell transplantation into male macaques will be performed at UC Davis. We expect to expand this study into a fully-independent project. ..
  15. Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative Stress
    Stuart A Meyers; Fiscal Year: 2010
    ....
  16. Molecular Analysis of Alagille Syndrome
    NANCY SPINNER; Fiscal Year: 2006
    ..abstract_text> ..