Top Publications

  1. pmc Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner
    Gregory N Bancroft
    Department of Bioengineering, Rice University, 6100 Main, Houston, TX 77005, USA
    Proc Natl Acad Sci U S A 99:12600-5. 2002
  2. pmc Microfluidic local perfusion chambers for the visualization and manipulation of synapses
    Anne M Taylor
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Neuron 66:57-68. 2010
  3. doi Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
    Harald C Ott
    Department of Surgery, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Boston, Massachusetts 02114, USA
    Nat Med 14:213-21. 2008
  4. doi Normothermic ex vivo lung perfusion in clinical lung transplantation
    Marcelo Cypel
    Toronto Lung Transplant Program, University of Toronto, ON, Canada
    N Engl J Med 364:1431-40. 2011
  5. ncbi Effects of topical hypotensive drugs on circadian IOP, blood pressure, and calculated diastolic ocular perfusion pressure in patients with glaucoma
    Luciano Quaranta
    Clinica Oculistica, Universita degli Studi di Brescia, Brescia, Italy
    Invest Ophthalmol Vis Sci 47:2917-23. 2006
  6. doi Pittfalls of MRI measurement of white matter perfusion based on arterial spin labeling
    P van Gelderen
    Advanced MRI, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892 1065, USA
    Magn Reson Med 59:788-95. 2008
  7. doi Retrograde and antegrade cerebral perfusion: results in short elective arch reconstructive times
    Rita Karianna Milewski
    Division of Cardiovascular Surgery, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Ann Thorac Surg 89:1448-57. 2010
  8. doi Role of perfusion CT in assessing tumor blood flow and malignancy level of gastric cancer
    Asami Satoh
    Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chuo Ku, Chiba, Japan
    Dig Surg 27:253-60. 2010
  9. doi Computed tomography and magnetic resonance perfusion imaging in ischemic stroke: definitions and thresholds
    Krishna A Dani
    Department of Neurology, Institute of Neurological Sciences, University of Glasgow Southern General Hospital, Glasgow, Scotland
    Ann Neurol 70:384-401. 2011
  10. doi Video rate optoacoustic tomography of mouse kidney perfusion
    Andreas Buehler
    Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Ingoldstädter Landstrasse 1, 85764 Neuherberg, Germany
    Opt Lett 35:2475-7. 2010

Detail Information

Publications414 found, 100 shown here

  1. pmc Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner
    Gregory N Bancroft
    Department of Bioengineering, Rice University, 6100 Main, Houston, TX 77005, USA
    Proc Natl Acad Sci U S A 99:12600-5. 2002
    ..To investigate this phenomenon in vitro, marrow stromal osteoblasts were cultured on 3D scaffolds under flow perfusion with different rates of flow for an extended period to permit osteoblast differentiation and significant matrix ..
  2. pmc Microfluidic local perfusion chambers for the visualization and manipulation of synapses
    Anne M Taylor
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Neuron 66:57-68. 2010
    ..Here we developed a microfluidic local perfusion (microLP) chamber to access and manipulate synaptic regions and presynaptic and postsynaptic compartments in ..
  3. doi Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
    Harald C Ott
    Department of Surgery, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Boston, Massachusetts 02114, USA
    Nat Med 14:213-21. 2008
    ..We decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular ..
  4. doi Normothermic ex vivo lung perfusion in clinical lung transplantation
    Marcelo Cypel
    Toronto Lung Transplant Program, University of Toronto, ON, Canada
    N Engl J Med 364:1431-40. 2011
    ..With the use of normothermic ex vivo lung perfusion (EVLP), the retrieved donor lung can be perfused in an ex vivo circuit, providing an opportunity to reassess its ..
  5. ncbi Effects of topical hypotensive drugs on circadian IOP, blood pressure, and calculated diastolic ocular perfusion pressure in patients with glaucoma
    Luciano Quaranta
    Clinica Oculistica, Universita degli Studi di Brescia, Brescia, Italy
    Invest Ophthalmol Vis Sci 47:2917-23. 2006
    ..2%, dorzolamide 2%, and latanoprost 0.005% on intraocular pressure (IOP), blood pressure (BP), and diastolic ocular perfusion pressure (DOPP), calculated as the difference between the diastolic blood pressure (DBP) and IOP.
  6. doi Pittfalls of MRI measurement of white matter perfusion based on arterial spin labeling
    P van Gelderen
    Advanced MRI, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892 1065, USA
    Magn Reson Med 59:788-95. 2008
    Although arterial spin labeling (ASL) MRI has been successfully applied to measure gray matter (GM) perfusion in vivo, accurate detection of white matter (WM) perfusion has proven difficult...
  7. doi Retrograde and antegrade cerebral perfusion: results in short elective arch reconstructive times
    Rita Karianna Milewski
    Division of Cardiovascular Surgery, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Ann Thorac Surg 89:1448-57. 2010
    ..Both retrograde cerebral perfusion with deep hypothermic circulatory arrest (RCP/DHCA) and antegrade cerebral perfusion with moderate hypothermic ..
  8. doi Role of perfusion CT in assessing tumor blood flow and malignancy level of gastric cancer
    Asami Satoh
    Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chuo Ku, Chiba, Japan
    Dig Surg 27:253-60. 2010
    Intratumoral hemodynamics or tumor perfusion is useful in understanding the pathological background of the cancer. A parameter for a non-invasive, preoperative assessment of tumor perfusion has yet to be developed.
  9. doi Computed tomography and magnetic resonance perfusion imaging in ischemic stroke: definitions and thresholds
    Krishna A Dani
    Department of Neurology, Institute of Neurological Sciences, University of Glasgow Southern General Hospital, Glasgow, Scotland
    Ann Neurol 70:384-401. 2011
    Cerebral perfusion imaging with computed tomography (CT) or magnetic resonance (MR) is widely available. The optimum perfusion values to identify tissue at risk of infarction in acute stroke are unclear...
  10. doi Video rate optoacoustic tomography of mouse kidney perfusion
    Andreas Buehler
    Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Ingoldstädter Landstrasse 1, 85764 Neuherberg, Germany
    Opt Lett 35:2475-7. 2010
    ..The previously undocumented capacity of whole-body optoacoustic tomography at a video rate has been demonstrated by visualizing mouse kidney perfusion using Indocyanine Green in vivo.
  11. doi Myocardial perfusion imaging is feasible for infarct size quantification in mice using a clinical single-photon emission computed tomography system equipped with pinhole collimators
    Tim Wollenweber
    Department of Nuclear Medicine, University of Munich, Munich, Germany
    Mol Imaging Biol 12:427-34. 2010
    The aim of this study is to evaluate a non-invasive method for measuring myocardial perfusion defect size in mice using a clinical single-photon emission computed tomography system equipped with pinhole collimators (pinhole SPECT).
  12. doi In vivo Gd-DTPA concentration for MR lung perfusion measurements: assessment with computed tomography in a porcine model
    Michael Puderbach
    Department of Radiology, German Cancer Research Center, Heidelberg, Germany
    Eur Radiol 18:2102-7. 2008
    ..MR signal and contrast-agent concentration (CAC) of the arterial-input function (AIF) is crucial for MR lung-perfusion quantification...
  13. ncbi Assessment of differential pulmonary blood flow using perfusion magnetic resonance imaging: comparison with radionuclide perfusion scintigraphy
    Francesco Molinari
    Department of Radiological Sciences, Catholic University of Rome, Rome, Italy
    Invest Radiol 41:624-30. 2006
    We sought to assess the agreement between lung perfusion ratios calculated from pulmonary perfusion magnetic resonance imaging (MRI) and those calculated from radionuclide (RN) perfusion scintigraphy.
  14. ncbi Do acute diffusion- and perfusion-weighted MRI lesions identify final infarct volume in ischemic stroke?
    C S Rivers
    Division of Clinical Neurosciences, Western General Hospital, Edinburgh, EH4 2XU, UK
    Stroke 37:98-104. 2006
    An acute mismatch on diffusion-weighted MRI (DWI) and perfusion-weighted MRI (PWI) may represent the "tissue-at-risk...
  15. pmc Inhibition of Dll4-mediated signaling induces proliferation of immature vessels and results in poor tissue perfusion
    Jeffrey S Scehnet
    Department of Pathology, Keck School of Medicine, University of Southern California, 1441 Eastlake Avenue, Los Angeles, CA, USA
    Blood 109:4753-60. 2007
    ..thin caliber, a markedly reduced vessel lumen, markedly reduced pericyte recruitment, and deficient vascular perfusion. sDll4 similarly induced defective vascular response in tumor implants leading to reduced tumor growth...
  16. ncbi Implementation of quantitative perfusion imaging techniques for functional brain mapping using pulsed arterial spin labeling
    E C Wong
    Department of Radiology, University of California, San Diego, La Jolla 92037 7756, USA
    NMR Biomed 10:237-49. 1997
    We describe here experimental considerations in the implementation of quantitative perfusion imaging techniques for functional MRI using pulsed arterial spin labeling...
  17. ncbi Perfusion CT: a worthwhile enhancement?
    K A Miles
    Southernex Imaging Group, Wesley Hospital, Chasely Street, Auchenflower, Queensland 4066, Australia
    Br J Radiol 76:220-31. 2003
  18. ncbi EANM/ESC procedural guidelines for myocardial perfusion imaging in nuclear cardiology
    B Hesse
    Department of Clinical Physiology and Nuclear Medicine, Rigshospitalet, University Hospital of Copenhagen, Copenhagen, Denmark
    Eur J Nucl Med Mol Imaging 32:855-97. 2005
    The European procedural guidelines for radionuclide imaging of myocardial perfusion and viability are presented in 13 sections covering patient information, radiopharmaceuticals, injected activities and dosimetry, stress tests, imaging ..
  19. ncbi Magnetic resonance quantification of the myocardial perfusion reserve with a Fermi function model for constrained deconvolution
    M Jerosch-Herold
    Department of Radiology, University of Minnesota Medical School, Minneapolis 55455, USA
    Med Phys 25:73-84. 1998
    The myocardial perfusion reserve, defined as the ratio of hyperemic and basal myocardial blood flow, is a useful indicator of the functional significance of a coronary artery lesion...
  20. doi Machine perfusion or cold storage in deceased-donor kidney transplantation
    Cyril Moers
    Department of Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    N Engl J Med 360:7-19. 2009
    Static cold storage is generally used to preserve kidney allografts from deceased donors. Hypothermic machine perfusion may improve outcomes after transplantation, but few sufficiently powered prospective studies have addressed this ..
  21. doi Longitudinal MRI study of cortical thickness, perfusion, and metabolite levels in major depressive disorder
    Hanna Järnum
    Department of Radiology, Aalborg Hospital Aarhus University Hospital, Denmark
    Acta Psychiatr Scand 124:435-46. 2011
  22. doi Hypothermic machine preservation in human liver transplantation: the first clinical series
    J V Guarrera
    Center for Liver Disease and Transplantation, Department of Surgery, Columbia University Medical Center, New York, NY, USA
    Am J Transplant 10:372-81. 2010
    Hypothermic machine perfusion (HMP) is widely used to preserve kidneys for transplantation with improved results over cold storage (CS)...
  23. doi Regeneration and orthotopic transplantation of a bioartificial lung
    Harald C Ott
    Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Nat Med 16:927-33. 2010
    ..Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange...
  24. doi Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants
    Vasiliy N Goral
    Science and Technology, Corning Incorporated, Corning, New York 14831 0001, USA
    Lab Chip 10:3380-6. 2010
    We describe a perfusion-based microfluidic device for three-dimensional (3D) dynamic primary human hepatocyte cell culture...
  25. pmc Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix
    Basak E Uygun
    Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Nat Med 16:814-20. 2010, allowing efficient recellularization of the liver matrix with adult hepatocytes and subsequent perfusion for in vitro culture...
  26. pmc Comparing kidney perfusion using noncontrast arterial spin labeling MRI and microsphere methods in an interventional swine model
    Nathan S Artz
    Department of Medical Physics, Wisconsin Institute for Medical Research, University of Wisconsin Madison, 1111 Highland Ave, Madison, WI 53705, USA
    Invest Radiol 46:124-31. 2011
    ..of a flow-sensitive alternating inversion recovery-arterial spin labeling (FAIR-ASL) technique to track renal perfusion changes during pharmacologic and physiologic alterations in renal blood flow using microspheres as a gold ..
  27. doi Protective mechanisms of end-ischemic cold machine perfusion in DCD liver grafts
    Andrea Schlegel
    Swiss HPB and Transplant Center, Department of Surgery, University Hospital Zurich, Switzerland
    J Hepatol 58:278-86. 2013
    The aim of this study was to identify protective mechanisms of cold machine perfusion in liver grafts donated after cardiac death.
  28. pmc Perfusion network shift during seizures in medial temporal lobe epilepsy
    Karen M Sequeira
    Comprehensive Epilepsy Center, Division of Neurology, Department of Neurosciences, Medical University of South Carolina, Charleston, SC, USA
    PLoS ONE 8:e53204. 2013 static structural limbic compromise, it is unknown whether the limbic system undergoes dynamic regional perfusion network alterations during seizures. In this study, we aimed to investigate state specific (i.e...
  29. ncbi Influence of perfusion on hepatic MR diffusion measurement
    K G Hollingsworth
    University Department of Radiology, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 2QQ, UK
    NMR Biomed 19:231-5. 2006
    ..The factors that influence hepatic diffusion measurements are complex and are thought to include hepatic perfusion. This study assesses whether hepatic diffusion measurements are influenced by hepatic perfusion changes as this ..
  30. ncbi Bolus delay and dispersion in perfusion MRI: implications for tissue predictor models in stroke
    Fernando Calamante
    Radiology and Physics Unit, UCL Institute of Child Health, University College London, London, UK
    Magn Reson Med 55:1180-5. 2006
    b>Perfusion maps, which are calculated from dynamic-susceptibility contrast (DSC)-MRI data by deconvolution of the arterial input function (AIF), are commonly used to predict tissue infarction in acute stroke...
  31. ncbi Autologous bone marrow cell transplantation increases leg perfusion and reduces amputations in patients with advanced critical limb ischemia due to peripheral artery disease
    Berthold Amann
    Department of Medicine, Franziskuskrankenhaus, Berlin Vascular Center, Berlin, Germany
    Cell Transplant 18:371-80. 2009
    ..7 x 10(9)). Limb salvage was 59% at 6 months and 53% at last follow-up (mean 411 +/- 261 days, range 175-1186). Perfusion measured with ankle-brachial index (ABI) and transcutaneous oxygen tension (tcpO(2)) at baseline and after 6 ..
  32. ncbi Cerebral blood perfusion changes in multiple sclerosis
    Jens Wuerfel
    Institute of Neuroimmunology, Charite Universitatsmedizin Berlin, Germany
    J Neurol Sci 259:16-20. 2007
    ..Until recently, the detection of cerebral blood perfusion changes was technically challenging, and perfusion studies in multiple sclerosis patients yielded contradictory ..
  33. doi Diffusion-weighted imaging of the prostate and rectal wall: comparison of biexponential and monoexponential modelled diffusion and associated perfusion coefficients
    S F Riches
    MRI Unit, Royal Marsden NHS Foundation Trust, Surrey, UK
    NMR Biomed 22:318-25. 2009 an apparent diffusion coefficient (ADC) from the monoexponential model and a diffusion coefficient, perfusion coefficient and perfusion fraction from the biexponential model...
  34. ncbi Mild therapeutic hypothermia for postischemic vasoconstriction in the perfused rat liver
    H A Zar
    Safar Center for Resuscitation Research and the Department of Anesthesiology and Critical Care Medicine, University of Pittsburgh School of Medicine, Pennsylvania, USA
    Anesthesiology 90:1103-11. 1999
    ..a promising therapy being evaluated for various clinical situations, may suppress the formation of reactive oxygen species during reperfusion and may ameliorate microcirculatory perfusion failure (the "no-reflow phenomenon").
  35. pmc First-pass perfusion CMR two days after infarction predicts severity of functional impairment six weeks later in the rat heart
    Daniel J Stuckey
    Department of Physiology, Anatomy and Genetics, University of Oxford, UK
    J Cardiovasc Magn Reson 13:38. 2011
    ..In order to identify perfusion delays in the infarcted rat heart, here we present a method in which a T1 weighted MR image has been acquired ..
  36. doi Diagnostic performance of noninvasive myocardial perfusion imaging using single-photon emission computed tomography, cardiac magnetic resonance, and positron emission tomography imaging for the detection of obstructive coronary artery disease: a meta-anal
    Caroline Jaarsma
    Department of Cardiology, Maastricht University Medical Center, Maastricht, The Netherlands
    J Am Coll Cardiol 59:1719-28. 2012
    This study aimed to determine the diagnostic accuracy of the 3 most commonly used noninvasive myocardial perfusion imaging modalities, single-photon emission computed tomography (SPECT), cardiac magnetic resonance (CMR), and positron ..
  37. doi Human liver cell spheroids in extended perfusion bioreactor culture for repeated-dose drug testing
    Rui M Tostões
    ITQB UNL, Oeiras, Portugal
    Hepatology 55:1227-36. 2012
    ..In this work, an automated perfusion bioreactor was used to obtain and maintain human hepatocyte spheroids...
  38. pmc Quantitative MR evaluation of atrophy, as well as perfusion and diffusion alterations within hippocampi in patients with Alzheimer's disease and mild cognitive impairment
    Anna Zimny
    Department of General and Interventional Radiology, and Neuroradiology, Wroclaw Medical University, Wroclaw, Poland
    Med Sci Monit 19:86-94. 2013
    The aim of this study was to evaluate atrophy rates, perfusion, and diffusion disturbances within the hippocampus, which is the site of characteristic changes in Alzheimer's disease (AD) and mild cognitive impairment (MCI).
  39. doi Bilateral versus unilateral antegrade cerebral perfusion in arch reconstruction for aortic dissection
    Shuyang Lu
    Shanghai Institute of Cardiovascular Disease, Department of Cardiovascular Surgery, Zhongshan Hospital, Fudan University, Shanghai, China
    Ann Thorac Surg 93:1917-20. 2012
    The superiority of bilateral versus unilateral antegrade cerebral perfusion (ACP) has been the subject of much debate. This study aimed to compare the two methods of cerebral perfusion.
  40. ncbi Quantitation of renal perfusion using arterial spin labeling with FAIR-UFLARE
    N Karger
    Klinikum an der CAU zu Kiel, Klinik für Diagnostische Radiologie, Michaelisstr 9, 24105, Kiel, Germany
    Magn Reson Imaging 18:641-7. 2000
    Quantitative perfusion imaging of human kidneys was performed using arterial spin labeling MRI with a fast spin echo readout-sequence. Perfusion maps of centrally located single slices were obtained in axial and coronal orientations...
  41. ncbi Autonomic modulation of electrical restitution, alternans and ventricular fibrillation initiation in the isolated heart
    G Andre Ng
    Cardiology Group, Department of Cardiovascular Sciences, University of Leicester, Leicester, United Kingdom
    Cardiovasc Res 73:750-60. 2007
    ..We examined the effects of direct sympathetic (SS) and vagus (VS) nerve stimulation on electrical restitution, alternans and VF threshold (VFT) in a novel isolated rabbit heart preparation with intact dual autonomic innervation...
  42. ncbi Pulsed star labeling of arterial regions (PULSAR): a robust regional perfusion technique for high field imaging
    Xavier Golay
    Department of Neuroradiology, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore 308433
    Magn Reson Med 53:15-21. 2005
    Regional perfusion imaging (RPI) has recently been introduced as a potentially powerful technique to map the perfusion territories of patients with vascular diseases in a fully noninvasive manner...
  43. ncbi Arterial spin labeling perfusion fMRI with very low task frequency
    Jiongjiong Wang
    Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Magn Reson Med 49:796-802. 2003
    ..By contrast, drift effects are minimized in arterial spin labeling (ASL) perfusion contrast, primarily as a result of successive pairwise subtraction between images acquired with and without ..
  44. pmc The role of dynamic instability and wavelength in arrhythmia maintenance as revealed by panoramic imaging with blebbistatin vs. 2,3-butanedione monoxime
    Qing Lou
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130 4899, USA
    Am J Physiol Heart Circ Physiol 302:H262-9. 2012
    ..This relatively high dynamic instability leads to the self-termination of arrhythmia because of the sufficiently long wavelength under blebbistatin...
  45. ncbi Pediatric perfusion imaging using pulsed arterial spin labeling
    Jiongjiong Wang
    Department of Radiology, University of Pennsylvania, Philadelphia 19104, USA
    J Magn Reson Imaging 18:404-13. 2003
    To test the feasibility of pediatric perfusion imaging using a pulsed arterial spin labeling (ASL) technique at 1.5 T.
  46. pmc Heterogeneity of pulmonary perfusion as a mechanistic image-based phenotype in emphysema susceptible smokers
    Sara K Alford
    Departmentsof Radiology, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 107:7485-90. 2010
    ..We sought to test the hypothesis, via multidetector row CT (MDCT) perfusion imaging, that smokers showing early signs of emphysema susceptibility have a greater heterogeneity in regional ..
  47. doi Ex-vivo normothermic liver perfusion: an update
    Thomas Vogel
    Nuffield Department of Surgery, Oxford Transplant Centre, University of Oxford, Oxford, UK
    Curr Opin Organ Transplant 15:167-72. 2010
    ..In the case of the liver, a substantial number of organs are discarded because of a risk of primary nonfunction...
  48. ncbi Atrioventricular conduction with and without AV nodal delay: two pathways to the bundle of His in the rabbit heart
    William J Hucker
    Biomedical Engineering Department, Washington University, 1 Brookings Drive, St Louis, MO 63130, USA
    Am J Physiol Heart Circ Physiol 293:H1122-30. 2007
    ..In conclusion, optical maps and S-H intervals for SP/His activation suggest that AV conduction via SP bypasses the compact AV node via the lower nodal bundle, which may be utilized to achieve long-term ventricular synchronization...
  49. ncbi Application of perfusion culture system improves in vitro and in vivo osteogenesis of bone marrow-derived osteoblastic cells in porous ceramic materials
    Yichao Wang
    Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Tissue Eng 9:1205-14. 2003
    ..b>Perfusion culture has potential utility for many cell types in three-dimensional (3D) culture...
  50. ncbi 3-D computational modeling of media flow through scaffolds in a perfusion bioreactor
    Blaise Porter
    Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 315 Ferst Dr NW, Atlanta, GA 30332, USA
    J Biomech 38:543-9. 2005
    Media perfusion bioreactor systems have been developed to improve mass transport throughout three-dimensional (3-D) tissue-engineered constructs cultured in vitro...
  51. ncbi Chronic mild reduction of cerebral perfusion pressure induces ischemic tolerance in focal cerebral ischemia
    Kazuo Kitagawa
    Division of Strokology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    Stroke 36:2270-4. 2005
    ..We focused on collateral development after mild reduction of perfusion pressure to find an endogenous response of the vascular system that contributes to development of ischemic ..
  52. ncbi The potential of ferumoxytol nanoparticle magnetic resonance imaging, perfusion, and angiography in central nervous system malignancy: a pilot study
    Edward A Neuwelt
    Department of Neurology, Oregon Health and Science University, Portland, OR 97239, USA
    Neurosurgery 60:601-11; discussion 611-2. 2007
    ..Our objectives were to determine the optimum time of delayed contrast enhancement of ferumoxytol, and to compare ferumoxytol and gadolinium contrast agents for magnetic resonance angiography and perfusion.
  53. pmc Determination of comprehensive arterial blood inflow in abdominal-pelvic organs: impact of respiration and posture on organ perfusion
    Takuya Osada
    Department of Sports Medicine for Health Promotion, Tokyo Medical University, Tokyo, Japan
    Med Sci Monit 17:CR57-66. 2011
    ..However, little information exists regarding the influence of respiration (interplay between inspiration and expiration) and posture on BFAP...
  54. ncbi Preservation of steatotic livers: a comparison between cold storage and machine perfusion preservation
    Maud Bessems
    Surgical Laboratory, Department of Surgery, Academic Medical Center, Amsterdam, The Netherlands
    Liver Transpl 13:497-504. 2007
    ..Hypothermic machine perfusion (MP) has been introduced for preservation of donor livers instead of cold storage (CS), resulting in superior ..
  55. doi Subnormothermic machine perfusion protects steatotic livers against preservation injury: a potential for donor pool increase?
    Mariapia Vairetti
    Department of Internal Medicine and Therapeutics, University of Pavia, Pavia, Italy
    Liver Transpl 15:20-9. 2009
    We tested whether rat liver preservation performed by machine perfusion (MP) at 20 degrees C can enhance the functional integrity of steatotic livers versus simple cold storage...
  56. doi Perfusion CT of the brain using 40-mm-wide detector and toggling table technique for initial imaging of acute stroke
    Sung Won Youn
    Department of Radiology, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam si 463 707, Korea
    AJR Am J Roentgenol 191:W120-6. 2008
    Limited coverage of the brain in the z-axis has been a drawback of perfusion CT...
  57. ncbi Convection perfusion of glucocerebrosidase for neuronopathic Gaucher's disease
    Russell R Lonser
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA
    Ann Neurol 57:542-8. 2005
    ..Primates underwent serial magnetic resonance imaging during enzyme perfusion of the right frontal lobe or brainstem, were observed and then sacrificed after infusion completion...
  58. doi Retrograde heart perfusion: the Langendorff technique of isolated heart perfusion
    Robert M Bell
    The Hatter Cardiovascular Institute, University College London Hospital and Medical School, 67 Chenies Mews, London WC1E 6HX, UK
    J Mol Cell Cardiol 50:940-50. 2011
    ..The Langendorff perfusion apparatus is highly adaptable and this is reflected not only in the procedure's longevity but also in the number ..
  59. pmc Intravenous desmoteplase in patients with acute ischaemic stroke selected by MRI perfusion-diffusion weighted imaging or perfusion CT (DIAS-2): a prospective, randomised, double-blind, placebo-controlled study
    Werner Hacke
    Department of Neurology, University of Heidelberg, Heidelberg, Germany
    Lancet Neurol 8:141-50. 2009
    ..activator, has clinical benefit when given 3-9 h after the onset of the symptoms of stroke in patients with presumptive tissue at risk that is identified by magnetic resonance perfusion imaging (PI) and diffusion-weighted imaging (DWI).
  60. ncbi Three-dimensional perfusion culture of human adipose tissue-derived endothelial and osteoblastic progenitors generates osteogenic constructs with intrinsic vascularization capacity
    Arnaud Scherberich
    Department of Surgery and Research, Institute for Surgical Research and Hospital Management, University Hospital Basel, Hebelstrasse 20, CH 4031 Basel, Switzerland
    Stem Cells 25:1823-9. 2007
    ..from human lipoaspirates were seeded and cultured for 5 days in porous hydroxyapatite scaffolds by alternate perfusion through the scaffold pores, eliminating standard monolayer (two-dimensional [2D]) culture...
  61. ncbi Oscillatory shear stress stimulates endothelial production of O2- from p47phox-dependent NAD(P)H oxidases, leading to monocyte adhesion
    Jinah Hwang
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    J Biol Chem 278:47291-8. 2003
  62. doi Machine perfusion versus cold storage for preservation of kidneys from expanded criteria donors after brain death
    Jürgen Treckmann
    Clinic for General, Visceral and Transplantation Surgery, University Hospital Essen, Hufelandstrasse 55, Germany
    Transpl Int 24:548-54. 2011
    The purpose of this study was to analyze the possible effects of machine perfusion (MP) versus cold storage (CS) on delayed graft function (DGF) and early graft survival in expanded criteria donor kidneys (ECD)...
  63. ncbi Advantages of normothermic perfusion over cold storage in liver preservation
    Charles J Imber
    Nuffield Department of Surgery, Oxford University, Oxford, United Kingdom
    Transplantation 73:701-9. 2002
    ..we have used an extracorporeal circuit to preserve the liver with normothermic, oxygenated, sanguineous perfusion. In this study, we directly compared preservation by the standard method of simple cold storage in University of ..
  64. ncbi The third gas: H2S regulates perfusion pressure in both the isolated and perfused normal rat liver and in cirrhosis
    Stefano Fiorucci
    Dipartimento di Medicina Clinica, Patologia, Clinica di Gastroenterologia ed Endoscopia Digestiva, University of Perugia, Perugia, Italy
    Hepatology 42:539-48. 2005
    ..The reduction of CSE expression in the liver with cirrhosis contributes to the development of increased intrahepatic resistance and portal hypertension...
  65. pmc Penetrating arterioles are a bottleneck in the perfusion of neocortex
    Nozomi Nishimura
    Department of Physics, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 104:365-70. 2007
    ..We conclude that penetrating arterioles are a bottleneck in the supply of blood to neocortex, at least to superficial layers...
  66. ncbi Evidence for intestinal and liver epithelial cell injury in the early phase of sepsis
    Joep P M Derikx
    Department of Surgery, University Hospital Maastricht and Nutrition and Toxicology Research Institute NUTRIM, Maastricht University, Maastricht, The Netherlands
    Shock 28:544-8. 2007
    ..Our aim was to study (1) the relation between intramucosal perfusion and intestinal and hepatic cell damage in an early phase of sepsis and (2) the correlation of these parameters ..
  67. ncbi Diffusion and perfusion MRI: basic physics
    R Luypaert
    MR-Centre, Academisch Ziekenhuis, , Laarbeeklaan 101, 1090, Brussels, Belgium
    Eur J Radiol 38:19-27. 2001
    Diffusion and perfusion MR imaging are now being used increasingly in neuro-vascular clinical applications...
  68. ncbi Isolated heart perfusion according to Langendorff---still viable in the new millennium
    Monika Skrzypiec-Spring
    Department of Pharmacology, Medical University Wroclaw, Wroclaw, Poland
    J Pharmacol Toxicol Methods 55:113-26. 2007
    ..In the last decade the method has brought many important advances in many areas including ischemia-reperfusion injury, cell-based therapy and donor heart preservation for transplant...
  69. pmc Maintaining network activity in submerged hippocampal slices: importance of oxygen supply
    Norbert Hajos
    Department of Cellular and Network Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony u 43, 1083 Budapest, Hungary
    Eur J Neurosci 29:319-27. 2009
    ..These improvements to the recording conditions enable detailed studies of neurons under more realistic conditions of network activity, which are essential for a better understanding of neuronal network operation...
  70. ncbi Advanced hepatocellular carcinoma: CT perfusion of liver and tumor tissue--initial experience
    Dushyant V Sahani
    Department of Abdominal Imaging and Interventional Radiology, Massachusetts General Hospital, 55 Fruit St, White 270, Boston, MA 02114, USA
    Radiology 243:736-43. 2007
    To prospectively assess computed tomographic (CT) perfusion for evaluation of tumor vascularity of advanced hepatocellular carcinoma (HCC) and to correlate CT perfusion parameters with tumor grade and serum markers.
  71. doi Diffusion of docosahexaenoic and eicosapentaenoic acids through the blood-brain barrier: An in situ cerebral perfusion study
    Melissa Ouellet
    Laval University, Quebec, QC, Canada
    Neurochem Int 55:476-82. 2009
    ..Here, using an in situ brain perfusion technique in mice, we show that [(14)C]-DHA and [(14)C]-EPA readily cross the mouse blood-brain barrier (BBB) ..
  72. pmc Decellularized tissue-engineered blood vessel as an arterial conduit
    Clay Quint
    Department of Biomedical Engineering, Yale University, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 108:9214-9. 2011
    ..grown from banked porcine smooth muscle cells that were allogeneic to the intended recipient, using a biomimetic perfusion system...
  73. doi Hemorrhagic transformation of ischemic stroke: prediction with CT perfusion
    Richard I Aviv
    Division of Neuroradiology, Sunnybrook Health Science Centre, Toronto, Ontario, Canada
    Radiology 250:867-77. 2009
    To determine whether admission computed tomography (CT) perfusion-derived permeability-surface area product (PS) maps differ between patients with hemorrhagic acute stroke and those with nonhemorrhagic acute stroke.
  74. ncbi Intestinal permeability of chlorpyrifos using the single-pass intestinal perfusion method in the rat
    Thomas J Cook
    Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Toxicology 184:125-33. 2003
    ..of chlorpyrifos (CPF), an organothiophosphate pesticide, was investigated using the single-pass intestinal perfusion (SPIP) technique in male, Sprague-Dawley rats...
  75. ncbi Glucose-stimulated insulin secretion is coupled to the interaction of actin with the t-SNARE (target membrane soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein) complex
    Debbie C Thurmond
    Department of Biochemistry and Molecular Biology, Center for Diabetes Research, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Mol Endocrinol 17:732-42. 2003
  76. ncbi Effects of medium perfusion rate on cell-seeded three-dimensional bone constructs in vitro
    Sarah H Cartmell
    School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Tissue Eng 9:1197-203. 2003
    ..This study examined the effects of medium perfusion rate on cell viability, proliferation, and gene expression within cell-seeded 3D bone scaffolds...
  77. pmc Evidence that hypoxia markers detect oxygen gradients in liver: pimonidazole and retrograde perfusion of rat liver
    G E Arteel
    Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill 27599, USA
    Br J Cancer 72:889-95. 1995
    ..To answer this question, the binding patterns of the 2-nitroimidazole, pimonidazole, were compared following perfusion of surgically isolated rat livers in anterograde and retrograde directions...
  78. ncbi A working heart-brainstem preparation of the mouse
    J F Paton
    Department of Physiology, School of Medical Sciences, University of Bristol, UK
    J Neurosci Methods 65:63-8. 1996
    ..The WH-BP may provide an advantaged environment for analysis of both synaptic and cellular mechanisms within the medulla that regulate cardio-respiratory activity...
  79. ncbi Arterial spin labeling in combination with a look-locker sampling strategy: inflow turbo-sampling EPI-FAIR (ITS-FAIR)
    M Gunther
    Deutsches Krebsforschungszentrum DKFZ, Forschungsschwerpunkt Radiologische Diagnostik und Therapie E0201, Heidelberg, Germany
    Magn Reson Med 46:974-84. 2001
    Arterial spin labeling (ASL) permits quantification of tissue perfusion without the use of MR contrast agents...
  80. ncbi Tissue-engineered vascular grafts composed of marine collagen and PLGA fibers using pulsatile perfusion bioreactors
    Sung In Jeong
    School of Chemical Engineering, College of Engineering, Hanyang University, Seoul 133 791, Republic of Korea
    Biomaterials 28:1115-22. 2007
    ..The pulsatile perfusion system enhanced the SMCs and ECs proliferation...
  81. pmc Spatiotemporal dynamics of diffusional kurtosis, mean diffusivity and perfusion changes in experimental stroke
    Edward S Hui
    Research Imaging Institute, University of Texas Health Science Center at San Antonio, TX, USA
    Brain Res 1451:100-9. 2012
    ..The current study showed that DKI has the potential to complement existing stroke imaging techniques, particularly in the assessment of subacute to early chronic stroke evolution...
  82. pmc Reproducibility of renal perfusion MR imaging in native and transplanted kidneys using non-contrast arterial spin labeling
    Nathan S Artz
    Department of Medical Physics, University of Wisconsin Madison, Wisconsin Institute for Medical Research, Madison, Wisconsin 53705 2275, USA
    J Magn Reson Imaging 33:1414-21. 2011
    To examine both inter-visit and intra-visit reproducibility of a MR arterial spin labeling (ASL) perfusion technique in native and transplanted kidneys over a broad range of renal function.
  83. pmc Perfused multiwell plate for 3D liver tissue engineering
    Karel Domansky
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    Lab Chip 10:51-8. 2010
    ..To address this, we have developed a bioreactor that fosters maintenance of 3D tissue cultures under constant perfusion and we have integrated multiple bioreactors into an array in a multiwell plate format...
  84. pmc Reliability and precision of pseudo-continuous arterial spin labeling perfusion MRI on 3.0 T and comparison with 15O-water PET in elderly subjects at risk for Alzheimer's disease
    Guofan Xu
    GRECC, Madison VA Hospital, Madison, WI, USA
    NMR Biomed 23:286-93. 2010
    ..The ICCs were greater than 0.90 in pcASL global perfusion measurements for both the young and the elderly groups...
  85. ncbi Development of a perfusion fed bioreactor for embryonic stem cell-derived cardiomyocyte generation: oxygen-mediated enhancement of cardiomyocyte output
    Celine Bauwens
    Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada
    Biotechnol Bioeng 90:452-61. 2005
    ..We developed a single-step bioprocess for ES cell-derived cardiomyocyte production that enables both medium perfusion and direct monitoring and control of dissolved oxygen...
  86. doi Quantitative analysis of transmural gradients in myocardial perfusion magnetic resonance images
    G L T F Hautvast
    Philips Healthcare, Imaging Systems, MR, Best, The Netherlands
    Magn Reson Med 66:1477-87. 2011
    Conventional quantitative assessments of myocardial perfusion analyze the temporal relation between the arterial input function and the myocardial signal intensity curves, thereby neglecting the important spatial relation between the ..
  87. ncbi Assessment of the severity of liver disease and fibrotic change: the usefulness of hepatic CT perfusion imaging
    Kazuhiko Hashimoto
    Department of Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan
    Oncol Rep 16:677-83. 2006
    This study assessed the utility of CT perfusion for quantitative assessment of liver function and fibrosis...
  88. ncbi Measurement of cerebral perfusion with arterial spin labeling: Part 1. Methods
    Thomas T Liu
    Department of Radiology, University of California San Diego, San Diego, California 92037, USA
    J Int Neuropsychol Soc 13:517-25. 2007
    ..Recent advances in acquisition and analysis methods have improved the somewhat limited sensitivity of ASL to perfusion changes associated with neural activity...
  89. doi Chondrogenic differentiation of human bone marrow mesenchymal stem cells in chitosan-based scaffolds using a flow-perfusion bioreactor
    M L Alves da Silva
    3Bs Research Group Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Zona Industrial da Gandra, S Cláudio do Barco, 4806 909 Caldas das Taipas, Guimaraes, Portugal
    J Tissue Eng Regen Med 5:722-32. 2011
    ..We intended to find out whether the shear stress caused by perfusion of the medium through the constructs was capable of augmenting the differentiation process...
  90. doi Clinical value of absolute quantification of myocardial perfusion with (15)O-water in coronary artery disease
    Sami A Kajander
    Turku PET Centre, University of Turku, Turku, Finland
    Circ Cardiovasc Imaging 4:678-84. 2011
    The standard interpretation of perfusion imaging is based on the assessment of relative perfusion distribution. The limitations of that approach have been recognized in patients with multivessel disease and endothelial dysfunction...
  91. doi A multiphase model for tissue construct growth in a perfusion bioreactor
    R D O'Dea
    School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK
    Math Med Biol 27:95-127. 2010
    The growth of a cell population within a rigid porous scaffold in a perfusion bioreactor is studied, using a three-phase continuum model of the type presented by Lemon et al...
  92. ncbi Comparative effects of combretastatin A-4 disodium phosphate and 5,6-dimethylxanthenone-4-acetic acid on blood perfusion in a murine tumour and normal tissues
    R Murata
    Danish Cancer Society, Department of Experimental Clinical Oncology, Aarhus University Hospital
    Int J Radiat Biol 77:195-204. 2001
    To compare the ability of combretastatin A-4 disodium phosphate (CA4DP) and 5,6-dimethylxanthenone-4-acetic acid (DMXAA) to change tissue blood perfusion.
  93. doi Optimal location for arterial input function measurements near the middle cerebral artery in first-pass perfusion MRI
    Egbert J W Bleeker
    Department of Radiology, C J Gorter Center for High Field MRI, Leiden University Medical Center, Leiden, The Netherlands
    J Cereb Blood Flow Metab 29:840-52. 2009
    One of the main difficulties in obtaining quantitative perfusion values from dynamic susceptibility contrast-magnetic resonance imaging is a correct arterial input function (AIF) measurement, as partial volume effects can lead to an ..
  94. pmc Prognostic value of normal regadenoson stress perfusion cardiovascular magnetic resonance
    Benjamin H Freed
    Department of Medicine, University of Chicago, Chicago, IL, USA
    J Cardiovasc Magn Reson 15:108. 2013
    ..Although extensively studied in patients undergoing nuclear perfusion imaging (MPI), its use for perfusion cardiovascular magnetic resonance (CMR) is not well described...
  95. pmc A novel porous mechanical framework for modelling the interaction between coronary perfusion and myocardial mechanics
    A N Cookson
    Imaging Sciences and Biomedical Engineering Division, St Thomas Hospital, King s College London, SE1 7EH, UK
    J Biomech 45:850-5. 2012
    ..tissue is a central feature of cardiac physiology and must therefore be accounted for by models of coronary perfusion. Currently available geometrically explicit vascular models fail to capture this interaction satisfactorily, are ..
  96. ncbi Identification of penumbra and infarct in acute ischemic stroke using computed tomography perfusion-derived blood flow and blood volume measurements
    B D Murphy
    University of Western Ontario, London, ON, Canada
    Stroke 37:1771-7. 2006
    We investigated whether computed tomography (CT) perfusion-derived cerebral blood flow (CBF) and cerebral blood volume (CBV) could be used to differentiate between penumbra and infarcted gray matter in a limited, exploratory sample of ..
  97. doi Optimization of culture conditions for osteogenically-induced mesenchymal stem cells in β-tricalcium phosphate ceramics with large interconnected channels
    Anne Bernhardt
    Max Bergmann Centre of Biomaterials and Institute for Materials Science, Technische Universitat Dresden, Germany
    J Tissue Eng Regen Med 5:444-53. 2011
    ..bone marrow-derived MSCs were seeded into the scaffolds and cultivated for up to 3 weeks in both static and perfusion culture in the presence of osteogenic supplements (dexamethasone, β-glycerophosphate, ascorbate)...
  98. doi "Dynamic spot sign" on CT perfusion source images predicts haematoma expansion in acute intracerebral haemorrhage
    Sheng jun Sun
    Department of Neuroradiology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
    Eur Radiol 23:1846-54. 2013
    To evaluate the association between dynamic progressive enhancing foci ("dynamic spot sign") in acute haematoma on CT perfusion source images (CTP-SI) and haematoma expansion.
  99. pmc Arterial spin labelling reveals an abnormal cerebral perfusion pattern in Parkinson's disease
    Tracy R Melzer
    Van der Veer Institute for Parkinson s and Brain Research, Christchurch 8011, New Zealand
    Brain 134:845-55. 2011
    ..tomography and single photon emission computed tomography studies have suggested patterns of abnormal cerebral perfusion in Parkinson's disease as potential functional biomarkers...
  100. doi Multislice perfusion of the kidneys using parallel imaging: image acquisition and analysis strategies
    Alexander G Gardener
    Sir Peter Mansfield Magnetic Resonance Centre, School of Physics and Astronomy, University of Nottingham, Nottingham, UK
    Magn Reson Med 63:1627-36. 2010
    ..recovery arterial spin labeling with parallel imaging acquisition is used to acquire single-shot, multislice perfusion maps of the kidney...
  101. doi A novel Schlemm's Canal scaffold increases outflow facility in a human anterior segment perfusion model
    Lucinda J Camras
    Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, Nebraska 68198 5840, USA
    Invest Ophthalmol Vis Sci 53:6115-21. 2012
    ..An intracanalicular scaffold (Hydrus microstent) designed to reduce intraocular pressure as a glaucoma treatment was tested in human anterior segments to determine changes in outflow facility (C)...

Research Grants77

  1. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
    ..Magnetic resonance imaging (MRI) with tissue tagging and gadolinium perfusion will be performed at 2 days and 7 days after I/R injury...
  2. Hybrid Optical/Doppler Imaging of Ischemia in NEC - a multicenter trial
    David A Benaron; Fiscal Year: 2010
    ..NEC) can be reliably detected early in the process using broadband optical spectroscopy sensitive to changes in perfusion of the gut...
  3. Effects of Standardized Aerobic Exercise-Training on Neurocognitive and Neurodege
    Thomas O Obisesan; Fiscal Year: 2010
    ..homeostasis, hypertension and endothelia dysfunction are precursors of arteriolosclerosis, decreased cerebral perfusion and oxygen deprivation, all of which may increase AD risk...
    Alex Brown; Fiscal Year: 2010
    ..and prostaglandins that have well established roles in physiologic and pathologic processes such as renal perfusion, gastric cytoprotection, hemostasis, cancer, host defense, inflammation, and asthma...
  5. Intrathoracic Pressure Regulator for Resuscitation
    Keith G Lurie; Fiscal Year: 2011
    ..cardiac output and blood pressure and b) uniquely lowering intracranial pressure thereby increasing cerebral perfusion. Phase 1 pre-clinical studies demonstrated significantly improved survival and neurological outcome without ..
  6. Roles of oxidation and inflammation in aortic stiffening
    David G Harrison; Fiscal Year: 2013 accommodating a portion of stroke volume during systole, reducing systolic pressure and maintaining systemic perfusion during diastole...
  7. Complement Activation in Pregnancy and Hypertension
    Jean F Regal; Fiscal Year: 2013
    ..These studies will use reduced uteroplacental perfusion pressure (RUPP) model in rat that produces placental ischemia in third trimester resulting in hypertension and ..
  8. CT Evaluation of the Myocardial Effects of Risk Factors
    Lilach O Lerman; Fiscal Year: 2013
    ..Multi-detector computed tomography (CT) will be used to quantify non-invasively myocardial regional perfusion, ischemia, and microvascular integrity, in conjunction with blood oxygen level- dependent magnetic resonance ..
    Vijay Sharma; Fiscal Year: 2013
    ..Myocardial perfusion imaging (MPI), a versatile tool in clinical diagnosis, plays an important role in the noninvasive assessment of ..
  10. Tumor Cell Arrest and Adhesion in the Microcirculation
    Bingmei M Fu; Fiscal Year: 2013
    ..On the basis of our preliminary studies, we shall use a newly developed in vivo single vessel perfusion/bending method that can create non-uniformly distributed shear rates/stresses along the vessel wall to test two ..
  11. Detection of Liver Fibrosis Using IVIM Diffusion and DCE MRI in HCV
    Bachir Taouli; Fiscal Year: 2013
    ..we would like to build and validate a predictive model based on advanced functional MRI metrics (diffusion and perfusion)...
  12. Mechanisms of Vasovagal Syncope
    Julian M Stewart; Fiscal Year: 2013 repeating studies after NOS inhibition with nitro-L-arginine (NLA) and after repletion of NO during perfusion with NLA+sodium nitroprusside (SNP). We will obtain skin biopsies for NOS isoform protein and expression...
  13. Astrocytic Ca2+ Signaling in the Ischemic Penumbra
    Maiken Nedergaard; Fiscal Year: 2011
    ..Combined imaging of capillary flow and Ca2+ or NADH imaging will allow a correlation between local perfusion and astrocytic Ca2+ signaling or NADH on a single cell level in live animals...
  14. Real-Time Optical Diagnosis of Inflammatory Bowel Disease using SFDI
    Sylvain Gioux; Fiscal Year: 2013
    ..advances in structured illumination, will permit real-time quantification of tissue optical properties, inclding perfusion, hydration, and metabolism during colonoscopy, thus improving IBD detection...
  15. Endothelium-Myocardium Interaction in Netrin-1 Induced Cardioprotection
    HUA LINDA CAI; Fiscal Year: 2013
    ..potent cardioprotective effect of netrin-1 and the signaling mechanisms involved, using an ex vivo Langendorff perfusion system for cardiac I/R injury...
  16. Arm Exercise versus Other Stress Test Modalities for Clinical Outcome Prediction
    WADE HAMPTON MARTIN; Fiscal Year: 2013
    ..this project is that composite findings of arm ergometer exercise ECG stress testing, with selective myocardial perfusion imaging (MPI) in a subset of patients such as those with highly abnormal resting ECGs or blunted hemodynamic ..
  17. Basic and Clinical Studies of Noise-Induced and Age-Related Hearing Loss
    Sharon G Kujawa; Fiscal Year: 2012
    ..if acute excitotoxicity is the key upstream elicitor of the neuropathy, we exploit our techniques for cochlear perfusion in mouse to either 1) block noise-induced excitotoxicity with the glutamate antagonist DNQX (Aim 2a), 2) mimic ..
  18. Surgical Revascularization's Impact on Mitochondria in Hibernating Myocardium
    ROSEMARY FRANCES KELLY; Fiscal Year: 2013
    ..will be obtained to determine LIMA patency, recovery of regional wall thickening, and recovery of subendocardial perfusion. Also, studies of the recovery induced by surgical revascularization of hibernating myocardial blood flow, ..
  19. Experimental COPD: remediation, mitigation
    DONALD JOHN MASSARO; Fiscal Year: 2010
    ..g., hypoxia, inflammation (which may be caused by hypoxia), decreased perfusion and inadequate delivery of nutrients. Our hypothesis is that hypoxia, i.e...
  20. Monitoring of Cardiovascular Function in Infants By Transcutaneous Dye Dilution
    Istvan Seri; Fiscal Year: 2013
    ..In fact, clinical decisions about the treatment of low perfusion states (shock) in these patients are currently being made based primarily on blood pressure and other indirect, ..
  21. Role of Inflammation in the Pathobiology of Abdominal Aortic Aneurysm
    KATHLEEN RAMAN; Fiscal Year: 2013
    ..the M1 and M2 subtypes in the aortic tissues of RAGE null, sRAGE-treated, and untreated mice following elastase perfusion. We will confirm our findings with in vitro studies using primary bone marrow derived macrophages stimulated ..
  22. Homocysteine and Angiotensin II in Renovascular Remodeling
    Utpal Sen; Fiscal Year: 2013
    ..Barium sulfate-contrast X-ray angiography and renal cortical blood flow by moorFLPI full-filled laser perfusion imager. Renal function will be determined by measuring glomerular filtration rate (GFR)...
  23. Microvascular Dysfunction in Hyperhomocysteinemia
    Shawn E Bearden; Fiscal Year: 2012
    ..coordination of blood flow within microvascular networks and is an important mechanism for integrating tissue perfusion and peripheral resistance. Little is known of how disease alters CVD within a microvascular network...
  24. Aqueous humor dynamics studies in vivo and in vitro
    PAUL LEON KAUFMAN; Fiscal Year: 2010
    ..will be employed: aqueous humor formation by fluorophotometry;outflow facility by two-level constant pressure perfusion;uveoscleral outflow calculated from isotope dilution and accumulation measures;IOP by Goldmann applanation ..
  25. Experimental engineering of ovarian grafting to promote angiogenesis for fertilit
    GLENN LEWIS SCHATTMAN; Fiscal Year: 2010
    ..In Aim 2, we will test two experimental strategies for improving vascular perfusion and follicle survival by tissue engineering either at the future graft site or the graft itself...
  26. C. Elegans and Mitochondrial K+ Channels
    KEITH W NEHRKE; Fiscal Year: 2013
    ..Mitochondria are central to the pathogenesis of ischemia- perfusion injury and are believed to be a major target for preconditioning...
  27. Age-Related Decrease in A-Beta Peptide Clearance Pathways: CSF and BBB
    Conrad Earl Johanson; Fiscal Year: 2010
    ..CSF formation rate/volume will be determined by dilution of blue dextran in ventriculo-cisternal perfusion. Age-related changes in CP transport of tagged Ap and CSF turnover rate will be analyzed by ANCOVA and multiple ..
  28. Vascular Mechanisms in Pregnancy-Induced Hypertension
    Raouf A Khalil; Fiscal Year: 2013
    ..Reduction in uterine perfusion pressure (RUPP) in late pregnant rats is associated with HTN, increased vasoconstriction and reduced relaxation...
  29. Human hepatocytes for drug toxicity screening from Cardiac Death Donor livers
    Mark G Clemens; Fiscal Year: 2012
    ..human hepatocytes isolated from non-transplantable DCD livers through the modification of a hypothermic machine perfusion (HMP) resuscitation system that we have developed to restore function to ischemia damaged DCD livers for use in ..
  30. Role of Mitochondria Targeted CYP2E1 and HO-1 in Alcohol Mediated Tissue Injury
    Narayan G Avadhani; Fiscal Year: 2012
    ..Myocardial ischemia/reperfusion will be carried out using Langendorff perfusion system...
  31. Adjunctive Use of Apyrase to Fibrinolytic Therapy
    Ridong Chen; Fiscal Year: 2013 rigorously determine whether in the coronary fibrinolysis model in dogs, APT102 promotes improved myocardial perfusion and left ventricular function with minimal bleeding compared with clopidogrel...
  32. Bifunctional Redox Catalyst &Organic Nitrate for Limb Reperfusion
    ANDREW LURIE SALZMAN; Fiscal Year: 2013
    ..At 7, 14, and 21 days after reperfusion we will correlate limb perfusion (by Doppler flow), nerve conduction velocity ("NCV", per electromyography, "EMG"), and muscle strength (by foot ..
  33. Effect of Prone Position on Ventilator-Induced Lung Injury
    Guido Musch; Fiscal Year: 2013
    ..tracer kinetic modeling will be employed to measure regional metabolic activity of neutrophils, pulmonary perfusion and aeration in vivo...
  34. Neuroimmune modulation across the BBB: Regulation of ILS trafficking by TNF
    Weihong Pan; Fiscal Year: 2012
    ..We will identify the roles of IL15 receptors in transporting IL15 by use of in-situ brain perfusion studies in receptor knockout and wildtype mice, determine the effects of TNF on the transcellular permeability ..
  35. Novel non-invasive device for treatment of elevated intracranial pressures
    Keith G Lurie; Fiscal Year: 2011
    ..neuronal injury, advances are needed in the treatment of elevated intracranial pressure and decreased cerebral perfusion secondary brain injury...
    Itzhak Nissim; Fiscal Year: 2013
    ..using a rat model of fatty liver and/or MS and various systems, including (a) isolated hepatocytes;(b) a liver perfusion system;(c) isolated mitochondria;and (d) in vivo study...
  37. Perinatal Assessment of At-Risk Populations
    William P Fifer; Fiscal Year: 2013
    ..EEG and temperature, we propose to incorporate new state of the art measures of venous return and tissue perfusion, as we believe these parameters will provide more direct information about the physiological forces that drive ..
  38. Protein Handling By Renal Proximal Tubule Epithelial Cells
    Nicholas Ferrell; Fiscal Year: 2013
    ..We have developed a novel microfluidic bioreactor system for perfusion culture of renal epithelial cells with controlled shear stress and simulated filtrate composition...
  39. The Organ Specific Role of Superoxide Dismutase in Sepsis
    Alix Ashare; Fiscal Year: 2010
    ..vascular endothelial cells contributes to microvascular dysfunction in sepsis, which may lead to impaired tissue perfusion. Mitochondrial antioxidants are known to prevent cellular apoptosis in response to oxidative stress such as that ..
  40. Insulin action in human cardiac and skeletal muscle microvasculature
    Zhenqi Liu; Fiscal Year: 2013
    ..hypotheses that: 1) elevation of plasma FFAs blunts insulin-mediated cardiac and skeletal muscle microvascular perfusion and glucose uptake by activating inflammatory signaling and increasing endothelin 1 (ET- 1) secretion/action;2) ..
  41. Novel bundled mechanical and pharmacological CPR platform
    Anja Metzger; Fiscal Year: 2012
    ..pressure by immediate transference of the negative intrathoracic pressure to the brain, and increases cerebral perfusion pressure...
  42. Blood Pressure -- Determinants &Controllers
    Allen W Cowley; Fiscal Year: 2012
    ..Project 5 focuses on the microcirculation and the mechanisms that control and alter organ and tissue perfusion in hypertension and the impact that reductions in ANGllstimulated O2 inhibit the VEGF signaling pathway leading ..
  43. The Role of Complement in Airway Microvascular Disease
    MARK ROBERT NICOLLS; Fiscal Year: 2013
    ..Specific Aim 1 will be to determine how C3 activation impacts graft hypoxia, vascular perfusion and airway remodeling in transplant recipients and tests the hypothesis that the deleterious impact of C3 ..
  44. Surgical Indirect Revascularization For Symptomatic Intracranial Aterial Stenosis
    Nestor Gonzalez; Fiscal Year: 2013
    ..and allowing gradual development of collateral circulation where the brain demands it, deterring early hyper perfusion and hemorrhage...
  45. Volumetric Perfusion MRI of Liver Tumors to Detect Early Response to Chemotherapy
    Scott B Reeder; Fiscal Year: 2010
    ..agents in liver tumors, using new magnetic resonance imaging (MRI) methods that measure blood flow to tumors (perfusion)...
  46. Regulation of Intestinal Epithelial Tight Junction Barrier
    Thomas Y Ma; Fiscal Year: 2011
    ..consisting of filter-grown Caco-2 intestinal epithelial monolayers) and in-vivo (re-cycling intestinal perfusion) murine model systems...
    Julian H Lombard; Fiscal Year: 2012
    ..dramatic and widespread impairment of vascular relaxation mechanisms and an impaired ability to regulate tissue perfusion in the absence of an elevated blood pressure...
  48. Hypertension, Kidney and Pregnancy
    Joey P Granger; Fiscal Year: 2013
    ..endothelial factors will be examined in a conscious rat model of PE produced by long-term reductions in uterine perfusion pressure (RUPP)...
  49. Stem Cells For Myocardial Repair: A Large Bore Magnet Study
    Jianyi Zhang; Fiscal Year: 2012
    ..i) transdifferentiation of sMPC to cardiomyocytes, endothelial and, smooth muscle cells that improve BZ perfusion and function, ii ) a trophic effect: sMPC release cytokines that spare native cardiomyocytes from apoptosis and ..
  50. The Kidney, Hypertension, Pregnancy and Inflammation
    Babbette LaMarca; Fiscal Year: 2013
    ..We have shown that reduced uterine perfusion pressure (RUPP) in pregnant rats, a rat model of preeclampsia, is an important stimulus for the production of ..
  51. Resuscitation using novel impedance threshold devices in pediatrics
    Keith G Lurie; Fiscal Year: 2013
    ..draws venous blood back to the heart and lowers intracranial pressure, thereby increasing systemic and cerebral perfusion. Cerebral perfusion is increased by decreased intracranial resistance to forward blood flow secondary to higher ..
  52. Modulating Secondary Damage Following Traumatic Brain Injury in the Child
    Stuart H Friess; Fiscal Year: 2013
    ..that early stabilization of cerebral hypoperfusion following TBI with higher than currently accepted cerebral perfusion pressures and modulation of endothelin-1 signal transduction will reduce brain injury and improve functional ..
  53. Cardiovascular effects of GLP-1 in obesity/metabolic syndrome
    Johnathan D Tune; Fiscal Year: 2013
    ..These studies involve triple tracer PET approaches to measure basal and GLP-1 stimulated myocardial perfusion, total oxidation rate, and substrate utilization rates as well as quantificatin of the effects of GLP-1 on ..
    Michael Regnier; Fiscal Year: 2013
    ..mice (TG-R1R2) have elevated left ventricular (LV) function, measured by echocardiography and Langendorff perfusion. Here we propose a translational approach, i.e...
  55. Sex Differences in Myocardial Ischemia Triggered by Emotional Factors after MI
    Viola Vaccarino; Fiscal Year: 2010
    ..The aims of this study are: (1) Using single photon emission tomography (SPECT) [Tc-99m] sestamibi myocardial perfusion imaging, to compare myocardial perfusion during rest, during exercise, and during an emotionally stressful ..
  56. Calcium wave propagation along microvascular endothelial tubes
    MATTHEW JOHN SOCHA; Fiscal Year: 2012
    ..My overall goal is to apply the findings of this research to the development of novel strategies for treating vascular disease in which tissue perfusion is impaired.
  57. Establishing a neuroprotection model in the nonhuman primate to support translati
    MATTHEW SWAN LAWRENCE; Fiscal Year: 2013
    ..After an initial focal ischemia associated with reduced perfusion of the microvasculature of the optic disc, often in the setting of an anatomically susceptible disc and/or ..
    Itzhak Nissim; Fiscal Year: 2009
    ..will be performed using a rat model of fatty liver and/or MS and various systems, including (a) a liver perfusion system;(b) isolated mitochondria;and (c) in vivo study...
  59. Role of trabecular meshwork contractility in modulating outflow resistance
    CHEE HIAN TAN; Fiscal Year: 2013
    ..For Aim 1, an assay for outflow resistance using perfusion techniques will be established...
  60. Development of an In Vivo Stem Cell Tracking Method Using Ultrasound Imaging
    Flordeliza S Villanueva; Fiscal Year: 2010
    ..such as unfractionated adult bone marrow or marrow-derived mesenchymal stem cells (MSCs) has improved perfusion and indices of ventricular function...
  61. Fetal HCMV Infection: Role of the Human Placenta
    LENORE PALMA PEREIRA; Fiscal Year: 2013
    ..cytotrophoblasts (CTB) and blood vessels, and the chorionic villi become progressively edematous reducing blood perfusion. Accumulation of fluid in the placental-fetal unit also suggests lymphatic vessels in the decidua have ..
  62. African and European Descent, Ocular Blood Flow and Glaucoma Progression
    Alon Harris; Fiscal Year: 2013
    ..Given these findings, this study is designed to investigate if abnormal ocular perfusion pressure and blood flow in persons of AD with glaucoma contribute to the differences observed in the prevalence ..
  63. Temperature & Vasomotor Tone During the First 5 Days in Preterms Less than 1000g
    ROBIN BRITT KNOBEL; Fiscal Year: 2010
    ..and peripheral body temperature of 30 extremely low birth weight (ELBW) infants in relationship to peripheral perfusion during the first 5 days of life...
    CHARLES HALES; Fiscal Year: 2003
    ..Pulmonary embolism is common, yet frequently undiagnosed and fatal. Ventilation/perfusion lung scans are the most commonly used test...
  65. New Solutions to Improve Islet Recovery after Machine Preservation of Pancreas
    Michael Taylor; Fiscal Year: 2009
    ..b>Perfusion/preservation technology has been shown to have a major impact in circumventing ischemic injury in kidney ..
  66. Thoroughfare Channels in CBF Autoregulation at High ICP
    Edwin M Nemoto; Fiscal Year: 2009
    ..requiring precise clinical management of ICP control. Maintenance of cerebral perfusion pressure (CPP) >60 to 70 mmHg to ensure adequate brain perfusion is recommended based on cerebral blood flow (..
  67. Comparative effectiveness of noninvasive cardiac imaging
    Marcelo F Di Carli; Fiscal Year: 2010
    ..In addition to conventional imaging approaches (e.g., myocardial perfusion SPECT), newer imaging technology including coronary computed tomography angiography (CCTA), and positron ..
  68. Venous Ulcers: Testing Effects of Compression & Position
    Deidre Wipke Tevis; Fiscal Year: 2005
    ..aims are: Specific Aim 1) To measure baseline (supine) skin oxygen (02), skin carbon dioxide (C02), skin blood perfusion, and limb volume in the lower limbs of venous ulcer subjects and healthy adults; Specific Aim 2) To measure skin ..
  69. Nano-sized Cell Guidance System for Ischemic Tissue Repair
    Hyunjoon Kong; Fiscal Year: 2010
    ..of ischemic tissue via angiogenesis, vasculogenesis or both is being extensively studied to restore blood perfusion in various tissues...
    Sanjiv Kaul; Fiscal Year: 2004
    ..verbatim): Contrast-enhanced ultrasound has become a valuable experimental and clinical tool for assessing organ perfusion. It used gas-filled microbubbles as perfusion tracers, which scatter ultrasound during their transit through any ..
    Sanjiv Kaul; Fiscal Year: 2002
    ..verbatim): Contrast-enhanced ultrasound has become a valuable experimental and clinical tool for assessing organ perfusion. It used gas-filled microbubbles as perfusion tracers, which scatter ultrasound during their transit through any ..
  72. Effects of Vasoactive Drugs on Perfusion in Septic Shock
    Patrick Murray; Fiscal Year: 2005
    ..Standard indicators of adequate tissue perfusion which are used to titrate therapy in hypovolemic or cardiogenic shock are unreliable in hyperdynamic septic ..
  73. Bone Tissue Engineerig: Effect of Dynamic Perfusion
    Aaron Goldstein; Fiscal Year: 2009
    ..We propose that in vitro perfusion culture of osteoprogenitor cells remains a promising means for achieving clinically effective materials, but ..
  74. CTRIP Ex-vivo perfusion and ventilation of lungs to assess transplant suitability
    Thomas M Egan; Fiscal Year: 2010
    ..The aims of this proposal are: 1). to perfect a technique of lung perfusion and ventilation outside the body (ex-vivo) to reliably assess lung function and suitability for transplant;2)...
  75. Translation of pH-weighted MRI to Image Human Acute Stroke
    ALMA SORENSEN; Fiscal Year: 2009
    ..MRI, particularly perfusion and diffusion MRI, may highlight potentially salvageable ischemic tissue for treatment, and is being used as ..
    GILBERT NUSSBAUM; Fiscal Year: 1991
    ..assumed mechanism through which such improvement would be obtained is vasodilator-induced reduction of the tumor perfusion rate and/or increase in perfusion rates in critical normal tissues...
    Elias Melhem; Fiscal Year: 2001
    ..used in the sickle cell population, in an effort to document subcortical, as well as cortical, changes in perfusion and how these changes correlate with neuropsychological test performance...