western blotting


Summary: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

Top Publications

  1. ncbi Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein
    Ann M Sheehy
    Department of Microbiology, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nature 418:646-50. 2002
  2. ncbi Wnt5a signaling directly affects cell motility and invasion of metastatic melanoma
    Ashani T Weeraratna
    National Human Genome Research Institute, Cancer Genetics Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Cancer Cell 1:279-88. 2002
  3. ncbi Doublecortin is a microtubule-associated protein and is expressed widely by migrating neurons
    J G Gleeson
    Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, Boston, Massachusetts 02115, USA
    Neuron 23:257-71. 1999
  4. ncbi Discordant protein and mRNA expression in lung adenocarcinomas
    Guoan Chen
    Department of Surgery, University of Michigan, Ann Arbor, Michigan 48109, USA
    Mol Cell Proteomics 1:304-13. 2002
  5. pmc Plasminogen activator inhibitor-1 is a critical downstream target of p53 in the induction of replicative senescence
    Roderik M Kortlever
    Division of Molecular Carcinogenesis and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 1211066 CX Amsterdam, The Netherlands
    Nat Cell Biol 8:877-84. 2006
  6. pmc MicroRNA-192 in diabetic kidney glomeruli and its function in TGF-beta-induced collagen expression via inhibition of E-box repressors
    Mitsuo Kato
    Gonda Diabetes Center, Division of Molecular Biology, Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 104:3432-7. 2007
  7. ncbi The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress
    Amy S Lee
    Department of Biochemistry and Molecular Biology and the USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089 9176, USA
    Methods 35:373-81. 2005
  8. ncbi Negative control of p53 by Sir2alpha promotes cell survival under stress
    J Luo
    Institute of Cancer Genetics and Department of Pathology, College of Physicians and Surgeons, Columbia University, 1150 St Nicholas Avenue, New York, NY 10032, USA
    Cell 107:137-48. 2001
  9. ncbi Endoplasmic reticulum stress contributes to beta cell apoptosis in type 2 diabetes
    D R Laybutt
    Diabetes and Obesity Research Program, Garvan Institute of Medical Research, St Vincent s Hospital, Sydney, NSW, Australia
    Diabetologia 50:752-63. 2007
  10. ncbi Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta
    Motoyuki Itoh
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Dev Cell 4:67-82. 2003

Research Grants

Detail Information

Publications407 found, 100 shown here

  1. ncbi Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein
    Ann M Sheehy
    Department of Microbiology, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nature 418:646-50. 2002
    ..Because the Vif:CEM15 regulatory circuit is critical for HIV-1 replication, perturbing the circuit may be a promising target for future HIV/AIDS therapies...
  2. ncbi Wnt5a signaling directly affects cell motility and invasion of metastatic melanoma
    Ashani T Weeraratna
    National Human Genome Research Institute, Cancer Genetics Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Cancer Cell 1:279-88. 2002
    ..Furthermore, Wnt5a expression in human melanoma biopsies directly correlated to increasing tumor grade. These observations support a role for Wnt5a in human melanoma progression...
  3. ncbi Doublecortin is a microtubule-associated protein and is expressed widely by migrating neurons
    J G Gleeson
    Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, Boston, Massachusetts 02115, USA
    Neuron 23:257-71. 1999
    ..Therefore, DCX likely directs neuronal migration by regulating the organization and stability of microtubules...
  4. ncbi Discordant protein and mRNA expression in lung adenocarcinomas
    Guoan Chen
    Department of Surgery, University of Michigan, Ann Arbor, Michigan 48109, USA
    Mol Cell Proteomics 1:304-13. 2002
    ..Using a quantitative analysis of mRNA and protein expression within the same lung adenocarcinomas, we showed that only a subset of the proteins exhibited a significant correlation with mRNA abundance...
  5. pmc Plasminogen activator inhibitor-1 is a critical downstream target of p53 in the induction of replicative senescence
    Roderik M Kortlever
    Division of Molecular Carcinogenesis and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 1211066 CX Amsterdam, The Netherlands
    Nat Cell Biol 8:877-84. 2006
    ..Our data indicate that PAI-1 is not merely a marker of senescence, but is both necessary and sufficient for the induction of replicative senescence downstream of p53...
  6. pmc MicroRNA-192 in diabetic kidney glomeruli and its function in TGF-beta-induced collagen expression via inhibition of E-box repressors
    Mitsuo Kato
    Gonda Diabetes Center, Division of Molecular Biology, Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 104:3432-7. 2007
    ..These results uncover a role for miRs in the kidney and DN in controlling TGF-beta-induced Col1a2 expression by down-regulating E-box repressors...
  7. ncbi The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress
    Amy S Lee
    Department of Biochemistry and Molecular Biology and the USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089 9176, USA
    Methods 35:373-81. 2005
    ..This can be achieved by measuring the Grp78 promoter activity or by measuring the level of Grp78 transcripts or GRP78 protein. These techniques can be applied to tissue culture cells as well as tissues and organs...
  8. ncbi Negative control of p53 by Sir2alpha promotes cell survival under stress
    J Luo
    Institute of Cancer Genetics and Department of Pathology, College of Physicians and Surgeons, Columbia University, 1150 St Nicholas Avenue, New York, NY 10032, USA
    Cell 107:137-48. 2001
    ..These results have significant implications regarding an important role for Sir2alpha in modulating the sensitivity of cells in p53-dependent apoptotic response and the possible effect in cancer therapy...
  9. ncbi Endoplasmic reticulum stress contributes to beta cell apoptosis in type 2 diabetes
    D R Laybutt
    Diabetes and Obesity Research Program, Garvan Institute of Medical Research, St Vincent s Hospital, Sydney, NSW, Australia
    Diabetologia 50:752-63. 2007
    ..We investigated whether endoplasmic reticulum (ER) stress is necessary for lipid-induced apoptosis in beta cells and also whether ER stress is present in islets of an animal model of diabetes and of humans with type 2 diabetes...
  10. ncbi Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta
    Motoyuki Itoh
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Dev Cell 4:67-82. 2003
    ..This facilitates intramembranous cleavage of the remaining Notch receptor, release of the Notch intracellular fragment, and activation of target genes in neighboring cells...
  11. pmc Hypoxia-induced neutrophil survival is mediated by HIF-1alpha-dependent NF-kappaB activity
    Sarah R Walmsley
    Division of Respiratory Medicine, Department of Medicine, School of Clinical Medicine, University of Cambridge, Addenbrooke s and Papworth Hospitals, Cambridge, CB2 2QQ, UK
    J Exp Med 201:105-15. 2005
    ..Gene array, TaqMan PCR, Western blotting, and oligonucleotide binding assays identify NF-kappaB as a novel hypoxia-regulated and HIF-dependent target, ..
  12. ncbi Reverse phase protein array: validation of a novel proteomic technology and utility for analysis of primary leukemia specimens and hematopoietic stem cells
    Raoul Tibes
    Department of Medical Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030 4095, USA
    Mol Cancer Ther 5:2512-21. 2006
    ..amplification, and staining precision are very high, reproducible, and that they correlate with traditional Western blotting. Using replicates of the same sample on the same and/or separate arrays, or using separate protein samples ..
  13. ncbi Phosphatidylinositol 3-kinase/AKT-mediated activation of estrogen receptor alpha: a new model for anti-estrogen resistance
    R A Campbell
    Department of Surgery, Indiana University School of Medicine, Indianapolis 46202, USA
    J Biol Chem 276:9817-24. 2001
    ..Taken together, these results define a molecular link between activation of the PI 3-kinase/AKT survival pathways, hormone-independent activation of ERalpha, and inhibition of tamoxifen-induced apoptotic regression...
  14. pmc The nuclear factor HMGB1 mediates hepatic injury after murine liver ischemia-reperfusion
    Allan Tsung
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Exp Med 201:1135-43. 2005
    ..Together, these results demonstrate that HMGB1 is an early mediator of injury and inflammation in liver I/R and implicates TLR4 as one of the receptors that is involved in the process...
  15. ncbi LKB1 is the upstream kinase in the AMP-activated protein kinase cascade
    Angela Woods
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London W12 0NN, United Kingdom
    Curr Biol 13:2004-8. 2003
    ..These results identify a link between two protein kinases, previously thought to lie in unrelated, distinct pathways, that are associated with human diseases...
  16. pmc Persistent DNA damage signalling triggers senescence-associated inflammatory cytokine secretion
    Francis Rodier
    Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
    Nat Cell Biol 11:973-9. 2009
    ..Thus, in addition to orchestrating cell-cycle checkpoints and DNA repair, a new and important role of the DDR is to allow damaged cells to communicate their compromised state to the surrounding tissue...
  17. ncbi Conversion of proepithelin to epithelins: roles of SLPI and elastase in host defense and wound repair
    Jing Zhu
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA
    Cell 111:867-78. 2002
    ..Supplying PEPI corrects the wound-healing defect in SLPI null mice. Thus, SLPI/elastase act via PEPI/EPIs to operate a switch at the interface between innate immunity and wound healing...
  18. ncbi Molecular characterization of human prostate carcinoma cell lines
    Adrie van Bokhoven
    Department of Pathology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Prostate 57:205-25. 2003
    ..This study presents a comprehensive survey and characterization of available prostate carcinoma cell lines, most of which have been widely used but are incompletely characterized...
  19. ncbi Activating gene expression in mammalian cells with promoter-targeted duplex RNAs
    Bethany A Janowski
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    Nat Chem Biol 3:166-73. 2007
    ..These data show that, like proteins, hormones and small molecules, small duplex RNAs interact at promoters and can activate or repress gene expression...
  20. ncbi Identification of novel argonaute-associated proteins
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Curr Biol 15:2149-55. 2005
    ..The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage...
  21. ncbi Tissue distribution of protease resistant prion protein in variant Creutzfeldt-Jakob disease using a highly sensitive immunoblotting assay
    J D Wadsworth
    MRC Prion Unit and Department of Neurogenetics, Imperial College School of Medicine at St Mary s, Norfolk Place, W2 1PG, London, UK
    Lancet 358:171-80. 2001
    ..We aimed to improve immunoblotting methods for high sensitivity detection of PrP(Sc) to investigate the distribution of PrP(Sc) in a range of vCJD tissues...
  22. ncbi Significant association of Rho/ROCK pathway with invasion and metastasis of bladder cancer
    Takao Kamai
    Department of Urology, Dokkyo University School of Medicine, Tochigi 321 0293, Japan
    Clin Cancer Res 9:2632-41. 2003
    ..We investigated the roles of Rho and ROCK in bladder cancer...
  23. ncbi Western blotting
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA
    Methods 38:283-93. 2006
    b>Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance...
  24. ncbi The survival motor neuron protein in spinal muscular atrophy
    D D Coovert
    Department of Molecular Genetics, College of Biological Sciences, Ohio State University, Columbus 43210, USA
    Hum Mol Genet 6:1205-14. 1997
    ..We suggest that disruption of SMN(T) in type I patients results in loss of SMN from motor neurons, resulting in the degeneration of these neurons...
  25. pmc MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development
    Paul L Boutz
    Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, California 90095, USA
    Genes Dev 21:71-84. 2007
    ..These results indicate that miR-133 directly down-regulates a key splicing factor during muscle development and establishes a role for microRNAs in the control of a developmentally dynamic splicing program...
  26. ncbi ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation
    Tatsuya Nakamura
    Kimmel Cancer Center and Department of Microbiology, Jefferson Medical College, Philadelphia, PA 19107, USA
    Mol Cell 10:1119-28. 2002
    ..In parallel, H3-K4 is methylated, and histones H3 and H4 are acetylated at this promoter...
  27. ncbi The unfolded protein response is activated in Alzheimer's disease
    J J M Hoozemans
    Neurogenetics Laboratory, Academic Medical Center, University of Amsterdam, P O Box 22660, 1100 DD, Amsterdam, The Netherlands
    Acta Neuropathol 110:165-72. 2005
    ..Although the initial participation of the UPR in AD pathogenesis might be neuroprotective, sustained activation of the UPR in AD might initiate or mediate neurodegeneration...
  28. pmc Mitochondrial remodeling in adipose tissue associated with obesity and treatment with rosiglitazone
    Leanne Wilson-Fritch
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, 01601, USA
    J Clin Invest 114:1281-9. 2004
  29. pmc Modulation of TLR2 protein expression by miR-105 in human oral keratinocytes
    Manjunatha R Benakanakere
    Center for Oral Health and Systemic Disease, University of Louisville School of Dentistry, Louisville, Kentucky 40202, USA
    J Biol Chem 284:23107-15. 2009
    ..Further understanding of the role of miRNA in host responses may elucidate disease susceptibility and suggest new anti-inflammatory therapeutics...
  30. ncbi Vascular endothelial growth factor expression of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin through nuclear factor-kappa B activation in endothelial cells
    I Kim
    National Creative Research Initiatives Center for Cardiac Regeneration, and the Department of Urology, Chonbuk National University School of Medicine, Chonju, 560-180, Republic of Korea
    J Biol Chem 276:7614-20. 2001
    ..The existence of parallel inverse signaling implies that the induction of adhesion molecule expression by VEGF is very finely regulated...
  31. pmc HIF-1alpha induces cell cycle arrest by functionally counteracting Myc
    Minori Koshiji
    Laboratory of Human Carcinogenesis, NCI, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 23:1949-56. 2004
    ..Hence, we propose that Myc is an integral part of a novel HIF-1alpha pathway, which regulates a distinct group of Myc target genes in response to hypoxia...
  32. pmc Up-regulation of dicer, a component of the MicroRNA machinery, in prostate adenocarcinoma
    Simion Chiosea
    Department of Pathology, University of Pittsburgh, University of Pittsburgh Medical Center Presbyterian, C920 1, 200 Lothrop St, Pittsburgh, PA 15213, USA
    Am J Pathol 169:1812-20. 2006
    ..The presence of up-regulated microRNA machinery may predict the susceptibility of prostate adenocarcinoma to RNA interference-based therapy...
  33. ncbi Chromatin remodeling factors and BRM/BRG1 expression as prognostic indicators in non-small cell lung cancer
    Junya Fukuoka
    Laboratory of Population Genetics, Biometric Research Branch, Center for Bioinformatics, Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland, USA
    Clin Cancer Res 10:4314-24. 2004
  34. pmc Cyclin A is required at two points in the human cell cycle
    M Pagano
    Differentiation, Programme European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 11:961-71. 1992
    ..Cyclin A binds both cdk2 and cdc2, giving two distinct cyclin A kinase activities, one appearing in S phase, the other in G2. These results suggest that cyclin A defines novel control points of the human cell cycle...
  35. ncbi Mammalian SIRT1 represses forkhead transcription factors
    Maria Carla Motta
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 116:551-63. 2004
    ..We speculate how down-regulating these two classes of damage-responsive mammalian factors may favor long lifespan under certain environmental conditions, such as calorie restriction...
  36. ncbi Inhibition of nuclear factor kappaB activation in PC3 cells by genistein is mediated via Akt signaling pathway
    Yiwei Li
    Department of Pathology, Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Clin Cancer Res 8:2369-77. 2002
  37. ncbi Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors
    Carlos Lois
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 295:868-72. 2002
    ..We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species...
  38. ncbi Transient expression of doublecortin during adult neurogenesis
    Jason P Brown
    Department of Neurology, University of Regensburg, 93053 Regensburg, Germany
    J Comp Neurol 467:1-10. 2003
    ..This assumption is further supported by our observation that the number of DCX-expressing cells in the dentate gyrus was decreased with age according to the reduction of neurogenesis in the aging dentate gyrus previously reported...
  39. pmc The human homologs of checkpoint kinases Chk1 and Cds1 (Chk2) phosphorylate p53 at multiple DNA damage-inducible sites
    S Y Shieh
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genes Dev 14:289-300. 2000
  40. pmc The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44
    H Morrison
    Forschungszentrum Karlsruhe, Institute of Toxicology and Genetics, and University of Karlsruhe, Institute of Genetics, 76021 Karlsruhe, Germany
    Genes Dev 15:968-80. 2001
    ..At low cell density, merlin is phosphorylated, growth permissive, and exists in a complex with ezrin, moesin, and CD44. These data indicate that merlin and CD44 form a molecular switch that specifies cell growth arrest or proliferation...
  41. ncbi Protein expression profiles in pancreatic adenocarcinoma compared with normal pancreatic tissue and tissue affected by pancreatitis as detected by two-dimensional gel electrophoresis and mass spectrometry
    Jianjun Shen
    Department of Carcinogenesis, Science Park Research Division, The University of Texas M D Anderson Cancer Center, Smithville, Texas, USA
    Cancer Res 64:9018-26. 2004
    ..The newly identified proteins in pancreatic tumors may eventually serve as diagnostic markers or therapeutic targets...
  42. ncbi Association between Pak1 expression and subcellular localization and tamoxifen resistance in breast cancer patients
    Caroline Holm
    Division of Pathology, Department of Laboratory Medicine, Lund University, Malmo, Sweden
    J Natl Cancer Inst 98:671-80. 2006
    ..Here we studied associations between Pak1 expression and subcellular localization in tumor cells and tamoxifen resistance...
  43. ncbi Critical role of CDK2 for melanoma growth linked to its melanocyte-specific transcriptional regulation by MITF
    Jinyan Du
    Department of Pediatric Hematology Oncology, Dana Farber Cancer Institute and Boston Children s Hospital, Boston, MA 02115, USA
    Cancer Cell 6:565-76. 2004
    ..Collectively, these data indicate that CDK2 activity in melanoma is largely maintained at the transcriptional level by MITF, and unlike other malignancies, it may be a suitable drug target in melanoma...
  44. ncbi A 10-kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b
    W H Wu
    Department of Veterinary Science, South Dakota State University, Brookings, South Dakota 57007, USA
    Virology 287:183-91. 2001
    ..These results suggest that the 2b protein is virion associated and the principal product of ORF2...
  45. pmc c-Kit triggers dual phosphorylations, which couple activation and degradation of the essential melanocyte factor Mi
    M Wu
    Division of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 14:301-12. 2000
  46. ncbi Human PAD4 regulates histone arginine methylation levels via demethylimination
    Yanming Wang
    Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Science 306:279-83. 2004
    ..Moreover, PAD4 activity is linked with the transcriptional regulation of estrogen-responsive genes in MCF-7 cells. These data suggest that PAD4 mediates gene expression by regulating Arg methylation and citrullination in histones...
  47. pmc Hypoxia and acidosis activate cardiac myocyte death through the Bcl-2 family protein BNIP3
    Lori A Kubasiak
    Department of Molecular and Cellular Pharmacology, University of Miami Medical Center, Miami, FL 33136, USA
    Proc Natl Acad Sci U S A 99:12825-30. 2002
    ..Overexpression of wild-type BNIP3, but not a translocation-defective mutant, activated cardiac myocyte death only when the myocytes were acidic. This pathway may figure significantly in muscle loss during myocardial ischemia...
  48. ncbi Persistent activation of stat3 signaling induces survivin gene expression and confers resistance to apoptosis in human breast cancer cells
    Tanya Gritsko
    Molecular Oncology, H Lee Moffitt Cancer Center and Research Institute Department of Interdisciplinary Oncology, University of South Florida College of Medicine, Tampa, Florida, USA
    Clin Cancer Res 12:11-9. 2006
    ..To gain a better understanding of the role of constitutive Stat3 signaling in breast cancer progression, we evaluated the expression profile of potential Stat3-regulated genes that may confer resistance to apoptosis...
  49. ncbi Global analysis of protein expression in yeast
    Sina Ghaemmaghami
    Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California 94143 2240, USA
    Nature 425:737-41. 2003
    ..Many of these molecules, including essential proteins and most transcription factors, are present at levels that are not readily detectable by other proteomic techniques nor predictable by mRNA levels or codon bias measurements...
  50. pmc Coupling endoplasmic reticulum stress to the cell death program: role of the ER chaperone GRP78
    Rammohan V Rao
    Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, CA 94945 1400, USA
    FEBS Lett 514:122-8. 2002
    ..These results define a novel protective role for GRP78 in preventing ER stress-induced cell death...
  51. ncbi Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery
    Jaya Mishra
    Division of Nephrology and Hypertension, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229 3039, USA
    Lancet 365:1231-8. 2005
    ..We tested the hypothesis that neutrophil gelatinase-associated lipocalin (NGAL) is an early biomarker for ischaemic renal injury after cardiopulmonary bypass...
  52. pmc C/EBPalpha is a DNA damage-inducible p53-regulated mediator of the G1 checkpoint in keratinocytes
    Kyungsil Yoon
    Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh, NC 27695 7633, USA
    Mol Cell Biol 24:10650-60. 2004
    ..Our results uncover a novel role for C/EBPalpha as a p53-regulated DNA damage-inducible gene that has a critical function in the DNA damage G(1) checkpoint response in keratinocytes...
  53. pmc Potent rescue of human immunodeficiency virus type 1 late domain mutants by ALIX/AIP1 depends on its CHMP4 binding site
    Yoshiko Usami
    Program in Gene Function and Expression, University of Massachusetts Medical School, LRB 526, 364 Plantation Street, Worcester, MA 01605, USA
    J Virol 81:6614-22. 2007
    ..Our data establish that ALIX can have a dramatic effect on HIV-1 release and suggest that the ability to use ALIX may allow HIV-1 to replicate in cells that express only low levels of Tsg101...
  54. ncbi Pretreatment with paclitaxel enhances apo-2 ligand/tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis of prostate cancer cells by inducing death receptors 4 and 5 protein levels
    R Nimmanapalli
    Moffitt Cancer Center and Research Institute, University of South Florida, Tampa 33612, USA
    Cancer Res 61:759-63. 2001
    ..These findings indicate that up-regulation of DR4 and DR5 protein levels by treatment with paclitaxel enhances subsequent Apo-2L/TRAIL-induced apoptosis of human prostate cancer cells...
  55. pmc A monoclonal antibody against Wnt-1 induces apoptosis in human cancer cells
    Biao He
    Thoracic Oncology Laboratory, Department of Surgery, Comprehensive Cancer Center, University of California, San Francisco, CA 94115, USA
    Neoplasia 6:7-14. 2004
    ..Our results indicate that both monoclonal anti-Wnt-1 antibody and Wnt-1 siRNA inhibit Wnt-1 signaling and can induce apoptosis in human cancer cells. These findings hold promise as a novel therapeutic strategy for cancer...
  56. ncbi The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling
    E J Lowenstein
    Department of Pharmacology, New York University Medical Center, New York 10016
    Cell 70:431-42. 1992
    ..These results suggest that GRB2/sem-5 plays a crucial role in a highly conserved mechanism for growth factor control of ras signaling...
  57. ncbi Buthionine sulfoximine enhancement of arsenic trioxide-induced apoptosis in leukemia and lymphoma cells is mediated via activation of c-Jun NH2-terminal kinase and up-regulation of death receptors
    Duo Chen
    Division of Hematology Oncology, Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029 6547, USA
    Cancer Res 66:11416-23. 2006
    ..These observations suggest that a DR-mediated pathway activated by JNK is involved in apoptosis induced by treatment with As(2)O(3) plus BSO...
  58. ncbi Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein
    H Bueler
    Institut für Molekularbiologie I, Universitat Zurich, Switzerland
    Nature 356:577-82. 1992
    ..It is now feasible to determine whether mice devoid of PrPC can propagate prions and are susceptible to scrapie pathogenesis...
  59. ncbi Inhibition of STAT3 signaling induces apoptosis and decreases survivin expression in primary effusion lymphoma
    Yoshiyasu Aoki
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 101:1535-42. 2003
    ..Since constitutive STAT3 activation and survivin expression have been widely documented in different types of cancers, their linkage may extend to many malignancies and be critical to their pathogenesis...
  60. pmc Characterization of Dicer-deficient murine embryonic stem cells
    Elizabeth P Murchison
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 102:12135-40. 2005
    ..These studies provide a conditional model of RNAi deficiency in mammals that will permit the dissection of the biological roles of the RNAi machinery in cultured mammalian cells...
  61. ncbi Activation of Stat3 by receptor tyrosine kinases and cytokines regulates survival in human non-small cell carcinoma cells
    Lanxi Song
    Experimental Therapeutics, H Lee Moffitt Cancer Center and Research Insitute, University of South Florida College of Medicine Tampa, FL 33612, USA
    Oncogene 22:4150-65. 2003
    ..These results demonstrate a role of Stat3 in transducing survival signals downstream of tyrosine kinases such as Src, EGF-R, and c-Met, as well as cytokines such as IL-6, in human nonsmall cell lung cancers...
  62. ncbi Proinvasive properties of ovarian cancer ascites-derived membrane vesicles
    Laura E Graves
    Department of Cellular and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA
    Cancer Res 64:7045-9. 2004
    ..Our data suggest that vesicle-stimulated proteinase activation leads to increased extracellular matrix degradation, which may facilitate tumor cell invasion and metastasis...
  63. ncbi Sulfasalazine and BAY 11-7082 interfere with the nuclear factor-kappa B and I kappa B kinase pathway to regulate the release of proinflammatory cytokines from human adipose tissue and skeletal muscle in vitro
    Martha Lappas
    Department of Obstetrics and Gynecology, University of Melbourne and Mercy Perinatal Research Center, Mercy Hospital for Women, 126 Clarendon Street, East Melbourne, 3002 Victoria, Australia
    Endocrinology 146:1491-7. 2005
    ..and I kappa B kinase (IKK-beta) and insulin receptor-beta protein expression were assessed by ELISA and Western blotting, respectively...
  64. ncbi Inhibition of heme oxygenase-1 increases responsiveness of pancreatic cancer cells to anticancer treatment
    Pascal O Berberat
    Division of Pancreatic Surgery and Molecular Pancreatic Research, Department of General Surgery, Institute of Immunology, University of Heidelberg, Heidelberg, Germany
    Clin Cancer Res 11:3790-8. 2005
    ..Therefore, specific inhibition of HO-1 expression may be a new option in pancreatic cancer therapy and may be used as sensitizer to chemotherapy and radiotherapy...
  65. ncbi The induction of prostaglandin E2 production, interleukin-6 production, cell cycle arrest, and cytotoxicity in primary oral keratinocytes and KB cancer cells by areca nut ingredients is differentially regulated by MEK/ERK activation
    Mei Chi Chang
    Team of Biomedical Science, Chang Gung Institute of Technology, Kwei Shan, Taoyuan, Taiwan
    J Biol Chem 279:50676-83. 2004
    ..We used cultured primary keratinocytes and KB cells, RT-PCR, flow cytometry, Western blotting, and ELISA to evaluate whether alterations in early gene expression is crucial in the carcinogenic processes ..
  66. pmc Proteomics of early zebrafish embryos
    Vinzenz Link
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauer Str 108, 01307 Dresden, Germany
    BMC Dev Biol 6:1. 2006
    ..While genetic methods are readily available in zebrafish, protocols for two dimensional (2D) gel electrophoresis and proteomics have yet to be developed...
  67. ncbi Cdk5 phosphorylation of doublecortin ser297 regulates its effect on neuronal migration
    Teruyuki Tanaka
    Department of Neurosciences, University of California, San Diego, La Jolla, 92093, USA
    Neuron 41:215-27. 2004
    ..These results suggest that Dcx phosphorylation by Cdk5 regulates its actions on migration through an effect on microtubules...
  68. ncbi Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-gamma production, IgG avidity and antigen recognition by Western blotting
    B J A Paule
    Laboratorio de Imunologia, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Av Reitor Miguel Calmon, S N Vale do Canela, CEP 40140 100, Salvador BA, Brazil
    Vet Immunol Immunopathol 96:129-39. 2003
    ..Both humoral and cellular responses kinetics presented a similar pattern of activation/deactivation but necropsy results suggested that the IFN-gamma test would be a very specific marker of CLA status...
  69. ncbi Exosome-like vesicles with dipeptidyl peptidase IV in human saliva
    Yuko Ogawa
    Institute of Medicinal Chemistry, Hoshi University, Tokyo, Japan
    Biol Pharm Bull 31:1059-62. 2008
    ..Our results demonstrate that human whole saliva contains exosome-like vesicles; they might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity...
  70. doi CTGF enhances migration and MMP-13 up-regulation via alphavbeta3 integrin, FAK, ERK, and NF-kappaB-dependent pathway in human chondrosarcoma cells
    Tzu Wei Tan
    Department of Pharmacology, China Medical University, Taichung, Taiwan
    J Cell Biochem 107:345-56. 2009
    ..Taken together, our results indicated that CTGF enhances the migration of chondrosarcoma cells by increasing MMP-13 expression through the alphavbeta3 integrin, FAK, ERK, and NF-kappaB signal transduction pathway...
  71. ncbi Alterations in the focal adhesion kinase/Src signal transduction pathway correlate with increased migratory capacity of prostate carcinoma cells
    J K Slack
    Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    Oncogene 20:1152-63. 2001
  72. ncbi Disabled-2 inactivation is an early step in ovarian tumorigenicity
    Z Fazili
    Department of Biochemistry and Winship Cancer Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Oncogene 18:3104-13. 1999
    ..more than a dozen established tumor cell lines derived from breast and ovarian epithelial tumors examined by Western blotting, Dab2 expression was undetectable in 90% of these cell lines...
  73. pmc Immunodiagnosis of Echinococcus infections: confirmatory testing and species differentiation by a new commercial Western Blot
    M Liance
    Laboratoire de Parasitologie Mycologie, Hôpital Henri Mondor AP HP, et Université Paris XII, 94010 Creteil, France
    J Clin Microbiol 38:3718-21. 2000
    ..This study shows the usefulness of the commercially available Echinococcus Western Blot IgG for the serological confirmation of human echinococcosis...
  74. ncbi The mycosins of Mycobacterium tuberculosis H37Rv: a family of subtilisin-like serine proteases
    G D Brown
    Department of Medical Biochemistry MRC Centre for Molecular and Cellular Biology, University of Stellenbosch, 7505, Tygerberg, South Africa
    Gene 254:147-55. 2000
    ..Mycosin-2 was modified by cleavage in all three mycobacterial species. The multiplicity and constitutive expression of these proteins suggests that they have an important role in the biology of M. tuberculosis...
  75. ncbi Expression and function of vascular endothelial growth factor receptor-1 on human colorectal cancer cells
    Fan Fan
    Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030 4009, USA
    Oncogene 24:2647-53. 2005
  76. ncbi Angiogenesis-associated protein annexin II in breast cancer: selective expression in invasive breast cancer and contribution to tumor invasion and progression
    Meena R Sharma
    University of Pennsylvania, Philadelphia, PA 19104, USA
    Exp Mol Pathol 81:146-56. 2006
    ..These results suggest that annexin II may be an attractive target for new anti-angiogenic and anti-breast cancer therapies...
  77. ncbi Fibroblasts cocultured with keloid keratinocytes: normal fibroblasts secrete collagen in a keloidlike manner
    Ivor Jiun Lim
    Division of Plastic Surgery, Department of Surgery, National University of Singapore, Singapore 119074
    Am J Physiol Cell Physiol 283:C212-22. 2002
  78. doi Proteomic analysis of membrane microdomain-associated proteins in the dorsolateral prefrontal cortex in schizophrenia and bipolar disorder reveals alterations in LAMP, STXBP1 and BASP1 protein expression
    A T Behan
    Department of Psychiatry, Royal College of Surgeons in Ireland, RCSI ERC, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland
    Mol Psychiatry 14:601-13. 2009
    ..signal protein 1 (BASP1) and limbic system-associated membrane protein (LAMP)) for validation by western blotting. This revealed significantly increased expression of these proteins in SCZ (STXBP1 (24% difference; P<0...
  79. ncbi Axonal transport, amyloid precursor protein, kinesin-1, and the processing apparatus: revisited
    Orly Lazarov
    Department of Neurobiology, Pharmacology, and Physiology, The University of Chicago, Chicago, Illinois 60637, USA
    J Neurosci 25:2386-95. 2005
  80. pmc The nuclear factor-kappaB pathway controls the progression of prostate cancer to androgen-independent growth
    Ren Jie Jin
    Vanderbilt Prostate Cancer Center and Department of Urologic Surgery, Vanderbilt University Medical Center, A1329 MCN, 1161 21st Avenue South, Nashville, TN 37232 2765, USA
    Cancer Res 68:6762-9. 2008
    ..Thus, the NF-kappaB pathway may be a potential target for therapy against androgen-independent prostate cancer...
  81. ncbi ARPE-19, a human retinal pigment epithelial cell line with differentiated properties
    K C Dunn
    Department of Ophthalmology, University of California, Davis 95616, USA
    Exp Eye Res 62:155-69. 1996
    ..Our results demonstrate that ARPE-19 has structural and functional properties characteristic of RPE cells in vivo and suggest that this cell line will be valuable for in vitro studies of retinal pigment epithelium physiology...
  82. ncbi PRL-3 phosphatase is implicated in ovarian cancer growth
    Federica Polato
    Laboratory of Molecular Pharmacology, Department of Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy
    Clin Cancer Res 11:6835-9. 2005
    ..In the present study, we evaluated the expression of PRL-3 in ovarian cancer tissue and its role in ovarian cancer cell growth...
  83. ncbi Fibroblast growth factor 2 induction of the osteocalcin gene requires MAPK activity and phosphorylation of the osteoblast transcription factor, Cbfa1/Runx2
    Guozhi Xiao
    Department of Periodontics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109 1078, USA
    J Biol Chem 277:36181-7. 2002
    ..This study is the first demonstration that Runx2 is phosphorylated and activated by FGF-2 via the MAPK pathway and suggests that FGF-2 plays an important role in regulation of Runx2 function and bone formation...
  84. ncbi Overexpression of oncogenic STK15/BTAK/Aurora A kinase in human pancreatic cancer
    Donghui Li
    Department of Gastrointestinal Medical Oncology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Clin Cancer Res 9:991-7. 2003
    ..The purpose of this study was to investigate the expression and copy number alteration of STK15 in pancreatic cancer...
  85. pmc Megabase chromatin domains involved in DNA double-strand breaks in vivo
    E P Rogakou
    Laboratory of Molecular Pharmacology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 146:905-16. 1999
    ..The results further suggest the possible existence of units of higher order chromatin structure involved in monitoring DNA integrity...
  86. pmc Adiponectin stimulates angiogenesis by promoting cross-talk between AMP-activated protein kinase and Akt signaling in endothelial cells
    Noriyuki Ouchi
    Molecular Cardiology Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 279:1304-9. 2004
    ..These data indicate that adiponectin can function to stimulate the new blood vessel growth by promoting cross-talk between AMP-activated protein kinase and Akt signaling within endothelial cells...
  87. ncbi Identification and biochemical characterisation of a protein phosphatase 5 homologue from Plasmodium falciparum
    Christoph Lindenthal
    Department of Parasitology, Institute for Tropical Medicine, University of Tubingen, Wilhelmstrasse 27, 72074 Tubingen, Germany
    Mol Biochem Parasitol 120:257-68. 2002
    ..The function of PfPP5 is presently unclear, but like other PP5s of many eukaryotic organisms, it may have important regulatory functions in the parasite cell cycle...
  88. pmc Beta-catenin mutations in cell lines established from human colorectal cancers
    M Ilyas
    Cancer Genetics and Immunology Laboratory, Imperial Cancer Research Fund, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, OX3 9DU, United Kingdom
    Proc Natl Acad Sci U S A 94:10330-4. 1997
    ..All 23 cell lines had full-length beta-catenin protein that was detectable by Western blotting and that coprecipitated with E-cadherin...
  89. ncbi Disruption of methylarginine metabolism impairs vascular homeostasis
    James Leiper
    Centre for Clinical Pharmacology, Division of Medicine, University College London, London WC1E 6JJ, UK
    Nat Med 13:198-203. 2007
    ..Our results also suggest that DDAH inhibition could be harnessed therapeutically to reduce the vascular collapse associated with sepsis...
  90. pmc Preservation and expansion of the primate keratocyte phenotype by downregulating TGF-beta signaling in a low-calcium, serum-free medium
    Tetsuya Kawakita
    TissueTech, Inc, Ocular Surface Center, Miami, Florida 33176, USA
    Invest Ophthalmol Vis Sci 47:1918-27. 2006
    ..To demonstrate whether the original keratocyte phenotype is maintained with proliferative activity by suppressing TGF-beta signaling in rhesus monkey keratocytes expanded in a serum-free and low-[Ca2+] medium...
  91. doi Delivery of PTEN via a novel gene microcapsule sensitizes prostate cancer cells to irradiation
    Atsushi Tomioka
    Department of Urology, Kinki University School of Medicine, 377 2 Ohno Higashi, Osaka Sayama, Osaka 589 8511, Japan
    Mol Cancer Ther 7:1864-70. 2008
    ..These data show that GelaTen gene therapy, enabling radiosensitization, can potentially treat prostate cancers that have MMAC/PTEN gene alterations associated with radioresistance...
  92. ncbi Evasion of a single-step, chemotherapy-induced senescence in breast cancer cells: implications for treatment response
    Lynne W Elmore
    Department of Pathology, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298 230, USA
    Clin Cancer Res 11:2637-43. 2005
    ..Here, we test the hypothesis that evasion of senescence confers resistance to chemotherapeutic drugs and ionizing radiation...
  93. ncbi Focal adhesion kinase is upstream of phosphatidylinositol 3-kinase/Akt in regulating fibroblast survival in response to contraction of type I collagen matrices via a beta 1 integrin viability signaling pathway
    Hong Xia
    Department of Medicine, University of Minnesota, Minneapolis, 55455, USA
    J Biol Chem 279:33024-34. 2004
    ..Our data identify a novel role for FAK, functioning upstream of PI3K/Akt, in transducing a beta(1) integrin viability signal in collagen matrices...
  94. pmc The hDcp2 protein is a mammalian mRNA decapping enzyme
    Zuoren Wang
    Department of Cell Biology and Neuroscience, Rutgers University, 604 Allison Road, Piscataway, NJ 08854 8082, USA
    Proc Natl Acad Sci U S A 99:12663-8. 2002
    ..Therefore the mammalian homologue of the yeast Dcp2 protein is an mRNA decapping enzyme demonstrated to contain intrinsic decapping activity...
  95. pmc Inactivation of Btk by insertion of lacZ reveals defects in B cell development only past the pre-B cell stage
    R W Hendriks
    Department of Cell Biology and Genetics, Faculty of Medicine, Erasmus University Rotterdam, The Netherlands
    EMBO J 15:4862-72. 1996
    ..Our results show that Btk expression is essential at two distinct differentiation steps, both past the pre-B cell stage...
  96. pmc Upregulation of neutrophil gelatinase-associated lipocalin in oesophageal squamous cell carcinoma: significant correlation with cell differentiation and tumour invasion
    Haihua Zhang
    Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou, Guangdong Province, People s Republic of China
    J Clin Pathol 60:555-61. 2007
    ..Recently, an elevated NGAL expression was reported in several types of cancers. However, the characteristics of NGAL expression in oesophageal squamous cell carcinoma (ESCC) are still unknown...
  97. ncbi 17beta-oestradiol up-regulates longevity-related, antioxidant enzyme expression via the ERK1 and ERK2[MAPK]/NFkappaB cascade
    Consuelo Borras
    Department of Physiology, School of Medicine, University of Valencia, Spain
    Aging Cell 4:113-8. 2005
  98. ncbi Palmitoylated cysteine 192 is required for RhoB tumor-suppressive and apoptotic activities
    De An Wang
    Drug Discovery Program, H Lee Moffitt Cancer Center and Research Institute, Department of Interdisciplinary Oncology, Tampa, Florida 33612, USA
    J Biol Chem 280:19243-9. 2005
  99. pmc Protein phosphatase 4 regulates apoptosis in leukemic and primary human T-cells
    Mirna Mourtada-Maarabouni
    Institute for Science and Technology in Medicine and School of Life Sciences, Huxley Building, Keele University, Keele ST5 5BG, UK
    Leuk Res 33:1539-51. 2009
    ..PP4 activity significantly affects the mutation rate in leukemic T-cells, indicating that PP4 dysfunction may be important in the development and progression of leukemia...
  100. ncbi ATP hydrolysis is required for cohesin's association with chromosomes
    Prakash Arumugam
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Curr Biol 13:1941-53. 2003
    ..These Smc "heads" are connected by Scc1, creating a tripartite proteinaceous ring...
  101. ncbi Axonal transport of amyloid precursor protein is mediated by direct binding to the kinesin light chain subunit of kinesin-I
    A Kamal
    Howard Hughes Medical Institute, Department of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla 92093, USA
    Neuron 28:449-59. 2000
    ..We propose that one of the normal functions of APP may be as a membrane cargo receptor for kinesin-I and that KLC is important for kinesin-I-driven transport of APP into axons...

Research Grants114 found, 100 shown here

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. Optimization of DNA Repair by Abh8
    Lauren Endres; Fiscal Year: 2013
    ..Overall, these findings will form the basis of future studies into Abh8 regulation during the DDR. ..
  3. Cardiotrophin-Like Cytokine 1, a Candidate Molecule for the FSGS Factor
    Virginia J Savin; Fiscal Year: 2010
    ..We will test the sera of 4 patients with FSGS for the presence of CLC-1 using LC-MS/MS and Western blotting for CLC-1 using polyclonal antibodies...
  4. Novel Mitochondrial Mechanisms of Ethanol-Induced Cardiac Injury in Aged Females
    DONNA HOPE KORZICK; Fiscal Year: 2010
    ..b>Western blotting and qRT- PCR will be employed for mitochondrial ER subtype targeting and downstream signals;iTRAQ and ..
  5. Epigenetic mechanisms relevant to the pathogenesis of ALS
    Neil W Kowall; Fiscal Year: 2013
    ..We will measure LSD1 gene expression and activity levels using Real-Time PCR, Western blotting, immunofluorescent staining and by assessing transcriptional activity using a luciferase assay and the ..
  6. DNA Damage and Response in the Bladder Microenvironment.
    Bradley P Dixon; Fiscal Year: 2013
    ..This may lead to cancer of the bladder, especially in children who have their bladders surgically reconstructed. ..
  7. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2009
    ..Our research will be useful in the development of novel treatments for the most aggressive forms of the childhood cancer neuroblastoma. ..
  8. Steroid Responsive Mechanisms in the Ear
    DENNIS ROYAL TRUNE; Fiscal Year: 2012
    ..Of particular interest are immune suppression of inflammatory cytokines and related transcription factors compared to the regulation of ion transport pathways that control fluid homeostasis. ..
  9. Ethanol Regulation of Serum Glucocorticoid Kinase I
    BLAIR N COSTIN; Fiscal Year: 2012
    ..can be characterized by measuring Sgk1 protein and isoform levels following acute ethanol exposure through western blotting followed by densitometry analysis and the quantitative polymerase chain reaction (Q-rtPCR)...
  10. New insights in mechanisms of renal injury
    Balakuntalam S Kasinath; Fiscal Year: 2013
    ..Structural parameters, glomerular and tubular hypertrophy and changes in matrix proteins will be examined by western blotting, immunohistochemistry, and qRT-PCR. Glomerular filtration rate (GFR) and albuminuria will be measured...
  11. Iron in the pathogenesis of Friedreich's ataxia
    ..This potential contributing factor will be examined by Western blotting of frataxin precursors and the mature functional protein, and in a more robust approach, isotope-coded ..
  12. Ghrelin: Behavioral, Anatomical, and Physiological Components of Obesity
    BRETT JOHN WARREN TEUBNER; Fiscal Year: 2011
  13. Overcoming Temozolomide Resistance using MGMT-targeting Spherical Nucleic Acids
    TIMOTHY L SITA; Fiscal Year: 2013
    ..In vivo studies with orthotopic GBM mouse models will assess the combination treatment of MGMTi-SNAs and TMZ;we intend to (1) enhance intratumoral apoptosis, (2) reduce glioma formation, and (3) increase survival time. ..
  14. Identification of Proteins Important for Male Osteoporosis
    Hong Wen Deng; Fiscal Year: 2013
    ..Significant differentially expressed proteins identified will be verified by Western blotting using samples from the replication cohort...
  15. Nuclear-Cytoplasmic Shuttling of Foxc2 via 14-3-3 Determines Proximal Tubule Cell
    DIANA LYNNE GOLDEN; Fiscal Year: 2012
    ..Extensive examination of the mechanisms and potential therapeutic targets will advance the understanding and potential cure for AKI in proximal tubular regeneration. ..
  16. Mechanisms and Consequences of Reduced PPAR gamma in Pulmonary Hypertension
    C Michael Hart; Fiscal Year: 2013
    ..will be measured in the proposed models using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting. Vascular cell proliferation, vascular remodeling, and PH will be determined with techniques published by the ..
  17. Characterization of epsilon-sarcoglycan interacting proteins in mouse brain
    Yuqing Li; Fiscal Year: 2012
    ..The results should significantly increase our understanding of the pathophysiology of M-D, which can ultimately aid the development of therapeutic treatments for M-D patients and other dystonia and myoclonus patients. ..
  18. Modifiers of Cyst Progression in Polycystic Kidney Disease
    Phillip Darwin Bell; Fiscal Year: 2013
    ..Thus there is a compelling reason to focus on this disease and its effective treatment or cure, which would greatly benefit the VA patient population. ..
  19. Mitochondrial and Nuclear Mechanisms of Estrogen Receptor-Mediated Cardioprotecti
    DONNA HOPE KORZICK; Fiscal Year: 2012
    ..Apoptosis will be assessed through in vivo coronary ligation, western blotting for cellular ER subtype targeting and downstream signals, qRT- PCR for ER- mediated gene expression, and ..
  20. Genistein and Prostate Cancer
    Rajvir Dahiya; Fiscal Year: 2010
    ..of steroid receptor genes and tumor suppressor genes at the mRNA and protein levels using Northern blot, and Western blotting analysi respectively...
  21. Extracellular ubiquitin: role in myocyte apoptosis and myocardial remodeling
    Krishna Singh; Fiscal Year: 2010
  22. Mechanisms of Angiogenesis in Retinopathy of Prematurity
    Mary Elizabeth Hartnett; Fiscal Year: 2009
    ..g., dihydroethidium);and NADPH oxidase activation. ..
    ROBERT STAFFORD BRIDGES; Fiscal Year: 2012
    ..Overall, reproductive experience-induced shifts in endocrine activities may affect endocrine-related diseases and behaviors later in adulthood, i.e. breast cancer, autoimmune diseases, and postpartum mood disorders. ..
  24. Pilot Studies of the Effect of Aging on Mucociliary Clearance
    Lawrence E Ostrowski; Fiscal Year: 2013
    ..amount of secreted and total airway mucin in the lung will be quantified by agarose gel electrophoresis and Western blotting. 2c) To test the hypothesis that aged mice exhibit defects in the regulation of ion transport...
  25. Regulation of Astrocyte TIMP-1 in HIV-Associated Dementia
    JEREL ADAM FIELDS; Fiscal Year: 2011
    ..Our studies will provide a better understanding of the specific mechanistic contributions of activated astrocytes to HIV-1-neuropathogensis and neuroinflammation. ..
  26. Neuropeptide regulation of neurohypophyseal function
    Francis W Flynn; Fiscal Year: 2012
    ..The nuclear translocation of the neurokinin receptor to the nucleus is novel and has implications for a large number of pathologies, such as epilepsy, that are linked to this receptor system in the brain. ..
  27. Mechanisms of coronary microvascular dysfunction in metabolic syndrome
    Johnathan D Tune; Fiscal Year: 2012
    ..The goal of our research is to delineate the precise mechanisms of obesity-related cardiovascular disease and thus establish a rationale for therapeutic intervention. ..
  28. Abnormal Bladder Epithelial Cell Gene Expression in BPS/IC
    SUSAN F KEAY; Fiscal Year: 2013
    ..These studies should yield valuable information about the mechanism of aberrant gene expression/ differentiation in BPS/IC bladder cells, potentially leading to development of more effective therapies. ..
  29. Mechanisms of Angiogenesis of Retinopathy of Prematurity
    ..g., dihydroethidium);and NADPH oxidase activation. ..
  30. Hepatitis B virus replication and DDB1
    Betty L Slagle; Fiscal Year: 2013
    ..This important new knowledge will reveal virus-induced changes at the post-translational level, and is predicted to provide mechanistic insight into new strategies through which to treat chronic HBV infection and prevent liver disease. ..
  31. Mechanisms of Early Growth Response Factor 1 (Egr-1) Induction by HSV-1 Lytic Inf
    SHAOCHUNG VICTOR HSIA; Fiscal Year: 2010
    ..The completion of this proposal will shed light on the molecular mechanism of HSV-1 mediated Egr1 expression and assist to develop new strategy to prevent viral replication and blindness caused by HSV-1. ..
  32. The unique role of B-Raf in murine cardiac development and postnatal function
    Natasha N Chattergoon; Fiscal Year: 2013
    ..We will couple the results of B-Raf disruption to morphological, biochemical, and functional parameters of cardiac performance in the newborn and adult using echocardiographic measures. (End of Abstract) ..
  33. Arsenic, Histone Modification, and Transcription
    LYNN SHELDON; Fiscal Year: 2009
    ..abstract_text> ..
  34. Potentiation of Glycine Receptors by Ethanol
    LUIS GERARDO AGUAYO; Fiscal Year: 2010
  35. Pathogenesis of Postoperative Cognitive Dysfunction
    Zhongcong Xie; Fiscal Year: 2013
    ..This proposal aims to investigate an understudied topic in an innovative system by testing novel hypotheses. Our efforts would ultimately lead to safer surgical care and better post-operative outcomes for senior patients. ..
  36. Localization and characterization of the Clostridium difficile biofilm
    Adam Driks; Fiscal Year: 2013
    ..In Aim 2, we will examine possible mechanisms by which C. difficile biofilms may contribute to CDI. ..
    HANNAH CHASE KINNEY; Fiscal Year: 2013
    ..to each other and to the medullary 5-HT system in normative development and SIDS using immunocytochemical, western blotting, and other tissue techniques...
  38. Mitochondrial Mechanisms of Programmed Necrosis in the Aged Female Heart
    Alexandra M Machikas; Fiscal Year: 2013
    ..of all types of cell death will include infarct assessment using TTC TUNEL staining, DNA laddering, and western blotting for protein markers of cell death signaling pathways...
  39. Modulation of cancer prevention by social stress
    Hildegard M Schuller; Fiscal Year: 2010
    ..of xenograft size as well as the quantitative determination of molecular markers by real-time PCR and Western blotting accompanied by histopathology and immunohistochemistry...
  40. Regulation of the Endothelial Citrulline-Nitric Oxide Cycle
    Duane C Eichler; Fiscal Year: 2010
    ..siRNA;3) functional studies via enzyme activity and NO assays;4) fluorescence microscopy;5) real time PCR and western blotting;and 6) promoter analysis using mutagenesis, luciferase assays and EMSA studies...
  41. Surgical Revascularization's Impact on Mitochondria in Hibernating Myocardium
    ROSEMARY FRANCES KELLY; Fiscal Year: 2013
    ..By performing a surgical operation to resupply blood to the heart, our research will test whether these adaptations to ischemia are reversible and allow the heart to return to normal function, or if the adaptations are permanent. ..
  42. ATM Controls Genomic Stability in Pluripotent Stem Cells
    JASON MARK BECKTA; Fiscal Year: 2013
    ..b>Western blotting and flow cytometry revealed that inhibition of ATM halts apoptosis at 3 and 6 hours, but increases it at 18 ..
  43. Identifying Key Structural Elements in the Activation of the Arp2/3 Complex
    Patricia A Khuu; Fiscal Year: 2013
    ..Arp2/3 is also involved in metastasis as it is required in the formation of cellular structures such as lamellipodia of migrating cells and invadopodia of cancers cells [5, 6]. ..
  44. Regulation of Fibronectin Matrix Assembly by uPAR
    PAULA J MC KEOWN-LONGO; Fiscal Year: 2012
    Sung Wan Kim; Fiscal Year: 2012
    ..In this application, we propose to develop a novel bioreducible polymer to deliver VEGF gene to primary myoblasts for the treatment of acute myocardial infarct to address these short- comings of current therapeutic strategies. ..
  46. Validation of Novel Infertility Biomarker
    PETER SUTOVSKY SUTOVSKY; Fiscal Year: 2012
    ..and epifluorescence-light microscopic analysis of immunolabeled spermatozoa, and by semi-quantitative western blotting/densitometry technique...
  47. The role of SIRT1/FOXO4 pathway in podocyte apoptosis of diabetes mellitus
    PETER YENLUNG CHUANG; Fiscal Year: 2013
    ..This study could give new insight into the pathogenesis of diabetic nephropathy and provide targets for novel therapy. ..
  48. Downregulation of Rod Metabolism in Retinopathy of Prematurity
    JAMES DANIEL AKULA; Fiscal Year: 2010
    ..Markers of oxidative stress are assessed by ELISA and western blotting. qPCR is used to supplement these measures and to evaluate the expression of neurogenic and angiogenic growth ..
  49. In vitro Type Two Diabetes Mellitus Tissue Model to Investigate Insulin Resistanc
    KELLY ANNE BURKE; Fiscal Year: 2013
    ..abstract_text> ..
  50. Endothelial-reactive antibodies: a diagnostic test for Susac's syndrome
    WILLIAM JAMES WALDMAN; Fiscal Year: 2011
    ..The goal of this project is the development of such a test, which would facilitate prompt treatment and thus prevent or minimize residual defects associated with the disease. ..
  51. Combined use of green tea and quercetin in prostate cancer
    ..This novel treatment will retain the non-toxic character of green tea and at the same time increase its activity in chemoprevention of CaP. ..
  52. Role in Myopia Development of Retinal Pigment Epithelium - A New Therapeutic Targ
    Yan Zhang; Fiscal Year: 2013
    ..UC Berkeley and UC San Francisco offer world-class research environments and support for the preparation and training of young scientists for independent research careers. ..
  53. Molecular Regulation of Alpha-Synuclein Misfolding and Toxicity in Two Yeast Mode
    SHUBHIK DEBBURMAN; Fiscal Year: 2009
    ..association and toxicity, a- synuclein localization (via GFP microscopy), aggregation (via GFP microcopy, Western blotting, and differential centrifugation), and toxicity (via growth curves, serial plating, and yeast cell death ..
  54. Homocysteine and Angiotensin II in Renovascular Remodeling
    Utpal Sen; Fiscal Year: 2013
    ..abstract_text> ..
  55. Is symmetrical dimethylation of Arginine 2 of Histone H3 used as an epigenetic si
    Marjorie A Oettinger; Fiscal Year: 2010
    ..The experiments proposed here should define the roles of this modification and lead to insights into its possible role in both normal development and human disease. ..
  56. Mediators &prognostic value of muscle mass &function in chronic kidney disease
    Francis Perry Wilson; Fiscal Year: 2013
    ..Controls will be matched based upon age, race, gender eGFR, and baseline muscle mass but be selected from the lowest quintile. The primary analysis will use mixed-effects models clustered at the matched-pair level. ..
  57. Resolving the role of nicotine-mediated phosphorylation on pancreatic fibrosis
    Joao A Paulo; Fiscal Year: 2013
    ..A human PaSC cell line will be incubated with and without nicotine. Western blotting will assess proteins characteristic of PaSC activation and identify nAChR subunits that are present...
  58. Mechanisms of Reperfusion-induced Endothelial Injury
    Cuihua Zhang; Fiscal Year: 2010
    ..production, electrochemical detection of authentic NO, real time PCR of RNA transcripts, and Western Blotting to evaluate expression of key proteins in the models...
  59. A Validated Resource of Thyroid Cancer Cell Lines for Pathway Discovery
    JEFFREY ALLEN KNAUF; Fiscal Year: 2010
    ..screen of key oncogenes and tumor suppressor genes and high throughput pathway activation analysis by Western blotting)...
  60. Role of Sirt1 in Regulating Glucose Homeostasis and Insulin Action in Aging
    COLETTE M KNIGHT; Fiscal Year: 2012
    ..Most significantly this study will assess how Sirtl function is altered in aging and its relationship to metabolic processes. ..
  61. Abnormality in Gene Expression of Key Mediators of Vitamin A Action in COPD
    Salil K Das; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: The objective of this study is to elucidate the relationship between severity of emphysema in COPD patients and abnormality in the expression of key mediators of retinoid action in lung. (End of Abstract) ..
  62. Genome-Wide Analysis of RXR-alpha Binding In Mouse Liver Chromatin with ChIP-SEQ
    SAUL JOSEPH KARPEN; Fiscal Year: 2010
    ..Methodologies will include western blotting, ChIP qPCR, RNA quantitation by rtPCR, exploration by ChIP of other members of the transcription complexes, ..
  63. The Role of Cks Proteins in Mammalian Meiosis
    Charles H Spruck; Fiscal Year: 2010
    ..Uncovering the function(s) of Cks2 in mammalian meiosis could lead to a better understanding about how Ml is regulated, and help to define the underlying causes of infertility and birth defects in humans. ..
  64. Epigenetic regulation in neuropathic pain
    Zhonghui Guan; Fiscal Year: 2013
    ..My department has guaranteed lab space and 80% of my professional time for my research, neither of which is contingent upon my receipt of this career award. ..
  65. Prevention of EtOH-induced promotion of hepatocarcinogenesis by genistein/soy
    Martin J J Ronis; Fiscal Year: 2013
    ..the molecular mechanisms underlying DEN/EtOH induction of b-catenin, and interactions with dietary genistein/SPI, by analyzing gene expression using targeted microarrays, Western blotting, immunohistochemistry and immunoprecipitation.
  66. Bmi-1, a potential therapeutic target in ovarian cancer
    Resham Bhattacharya; Fiscal Year: 2013
    ..The proposed studies will validate Bmi-1 as an important new target using readily translatable nanoliposomal delivery methods with potential for therapy even in chemoresistant ovarian cancer. ..
  67. Interactions between P. aeruginosa and autophagy in Airway epithelial cells
    INDHIRA H DE LA ROSA; Fiscal Year: 2013
    ..containing the sequence of T3SS effector proteins and by evaluating autophagy induction or blockage via Western blotting, fluorescence microscopy and co-Immunoprecipitation...
  68. Role of trabecular meshwork contractility in modulating outflow resistance
    CHEE HIAN TAN; Fiscal Year: 2013
    ..Next a contractility assay involving histomorphometry, immunohistochemistry and Western blotting will be assembled...
  69. Prenatal Cannabis Exposure and Epigenetic Mechanisms Underlying Vulnerability to
    CLAUDIA VARGAS MORRIS; Fiscal Year: 2013
  70. Molecular and Cellular Determinants of the Exercise Pressor Reflex in CHF
    Irving H Zucker; Fiscal Year: 2013
    ..Furthermore, w believe that these data will uncover new targets for therapy in CHF. ..
  71. Characterization of Estrogen Sensitive Hypothalamic Glucose Sensing Neurons
    AMMY MARIE SANTIAGO; Fiscal Year: 2013
  72. Nutrient Restriction: Placental and Fetal Brain and Renal Outcomes and Mechanism
    Peter W Nathanielsz; Fiscal Year: 2012
    ..Clinicians will use the information to understand optimal life style and diet in pregnancy. ..
  73. Role of LRG-47 (Irgm1) in EAE
    Shidu Yan; Fiscal Year: 2013
  74. Role of miRNA-375 in Invasive Phenotype of Head and Neck Squamous Cell Carcinoma
    Lizandra Jimenez; Fiscal Year: 2013
    ..Functional validation of the phenotypic effects of the down-regulation of putative targets of miR-375 will be performed by siRNA knockdown and rescue of the targets in the transductant cells. ..
  75. Genetics and Functional Studies of Age-Related Macular Degeneration
    Kang Zhang; Fiscal Year: 2012
    ..PUBLIC HEALTH RELEVANCE: This grant application focuses on investigation of a major gene (HTRA1) that causes age-related macular degeneration, the leading cause of blindness with a significant public health impact. ..
  76. Androgen Modulation of Neurodegeneration in Dopamine Neurons
    Rebecca L Cunningham; Fiscal Year: 2010
    ..Nopage numbering necessary. Not to exceed 3 publications;2 for predoctoral candidates.) E3 Check if Appendix is included PHS 416-1 (Rev. 10/05) Page 4 Form Page 4 ..
  77. polr1c and polr1d mutant zebrafish as new models for Treacher Collins syndrome
    KRISTIN EMILY NOACK WATT; Fiscal Year: 2013
    ..Additionally, these aims will identify new therapies for the treatment of TCS in humans which could alleviate the need for extensive corrective surgeries. ..
  78. Invasion of glioblastoma multiforme (GBM) cells in organotypic brain slices
    ZEV ARI BINDER; Fiscal Year: 2013
    ..2, ?3, ?5, ?6?1, ?v?3, and ?v?5 and CD44 in GBM tumor cells will be determined using ELISA, PCR, and Western blotting techniques...
  79. Effect of Chronic Alcohol on Liver APC and Ag-Specific T-Cells
    KATHERINE BREMPELIS; Fiscal Year: 2013
    ..exposure on the cell biology of the liver APC populations by using multiple methods such as microscopy, western blotting, qRT-PCR, and in vitro cell assays to study antigen presentation pathways in the presence and absence of ..
  80. The Role of Polycystin 1 C-terminal Cleavage in the Regulation of Cyst Growth
    BENJAMIN J FLAHERTY; Fiscal Year: 2013
    ..We hypothesize that TAZ morpholino knockdown will prevent this rescue by the PC1-CTT and that expression of a constitutively active mutant of TAZ may be sufficient to restore the wild type phenotype in the absence of the PC1-CTT. ..
  81. Spinocerebellar Ataxia Type 2 Gene and Gene Product
    Stefan M Pulst; Fiscal Year: 2013
    ..Answers to these questions will help in the identification of new avenues towards treatments of SCA2 and other degenerative ataxias. ..
  82. Dietary prevention of prostate cancer
    CHENDIL DAMODARAN; Fiscal Year: 2013
  83. Wear Particle Disease and NF-kappa B Signaling
    Stuart B Goodman; Fiscal Year: 2013
    ..The proposed research is important, innovative and clinically relevant;it will suggest potential local pharmacological strategies to mitigate the chronic inflammatory reaction to wear particles and periprosthetic osteolysis. ..
  84. Palmitic Acid Regulation of Dendritic Cell Toll-Like Receptor 4 Signaling
    ..Once H2O2 mechanisms are defined, targeted therapeutics such as ROS scavengers, anti-oxidants, and signaling inhibitors can be combined with dietary interventions to achieve maximum health benefits. ..
  85. Integrative Mechanisms of Adipose Tissue Dysfunction In obesity
    ROSARIO G SCALIA; Fiscal Year: 2013
    ..We hope that the results of this work will advance our understanding of the integrated mechanisms that initiate and maintain adipose tissue inflammation and related metabolic disorders. ..
  86. Brain Pericyte and Amyloid-beta Peptide Interaction
    Donghui Zhu; Fiscal Year: 2013
    ..Using in vitro capillary-like structures, we will idenify the rate-limiting step and key-signaling molecules in pericyte adhesion and migration affected by A[unreadable]. The rate-limiting step would be the ideal target for therapies. ..
  87. Neural Control of Vasopressin Release
    J Thomas Cunningham; Fiscal Year: 2013
    ..The findings of these experiments could potentially alter the way that inappropriate vasopressin release is studied and conceptualized clinically. ..
  88. The Role of Tfam in Mitochondrial Biogenesis following sepsis
    Jeffrey Keenan; Fiscal Year: 2013
    ..Confirmation of this hypothesis will form the basis for future experimentation as well as the development of targeted therapies to improve outcomes in sepsis. ..
  89. Integrating cellular and molecular heterogeneity of TKI response in NSCLC cells
    PETER LEE FRICK; Fiscal Year: 2013
    ..I will answer these questions using protein arrays and quantitative western blotting. Each of the aims will provide novel information on the dynamics of erlotinib response in the PC9 clone model ..
  90. Cellular, molecular and functional characterization of proteasomes in EAE
    Oscar A Bizzozero; Fiscal Year: 2013
    ..Identification of the cell(s) with altered proteasome function will ultimately serve to design a therapeutic approach geared to decrease the toxic consequence of oxidative burden in EAE and MS. ..
  91. Nitric oxide pathway requirement for the correction of cardiomyopathy in muscular
    James Patrick Gonzalez; Fiscal Year: 2013
    ..cell type is required for proper function and rescue can be discerned through the use of immunofluorescence, western blotting, and experiments viewing pathway activity...
  92. Identifying novel therapeutic strategies in mutant NRAS melanoma
    Ha Linh Vu; Fiscal Year: 2013
    ..Our long-term objective is to improve the treatment options for NRAS mutant melanomas. ..
  93. Alpha2-adrenoceptors modulate TRPV4 and reduce inflammation-induced visceral pain
    MARTIN R WATTS; Fiscal Year: 2013
    ..By revealing mechanisms of pain associated with IBD, in addition to how this pain can be alleviated, this project could contribute to improved treatment options for patients with IBD. ..
  94. GABAergic inhibition in stress-enhanced fear learning
    EDWARD MEYER; Fiscal Year: 2013
    ..In parallel experiments, Western blotting techniques will be done t compare SEFL conditioning induced alterations in surface and intracellular levels ..
  95. Mechanisms Modulating the Association between the ENG Pathway and Preeclampsia
    MANDY JO SCHMELLA; Fiscal Year: 2013
    ..Ultimately, results from this project will improve our understanding of the pathophysiologic underpinnings of PE and may help to identify preventive, predictive, and therapeutic/modifiable targets for women at risk for PE. ..
  96. Identification of mechanisms underlying reduction of cocaine seeking by exercise
    YVONNE ENIYE OGBONMWAN; Fiscal Year: 2013
    ..abstract_text> ..
    Daniel Johnston; Fiscal Year: 2013
    ..The experiments will utilize hippocampal brain slices from rats, somatic and dendritic patch-clamp electrophysiology, and immunohistochemistry and western blotting.
  98. Stem Cell Based Therapy for Regenerative Endodontics
    George T J Huang; Fiscal Year: 2013
    ..If successful, a subsequent human trial may be carried out in the future and its success will promise a reduced need of traditional aggressive root canal therapies. ..
  99. DNA methylation in aging, race and prostate cancer
    Rajvir Dahiya; Fiscal Year: 2006
    ..Protein expression of ERs will be analyzed by immunohistochemistry (for localization) and western blotting (for quantitation)...
  100. Gene Networks for Prostate Cancer Progression
    Leif Peterson; Fiscal Year: 2005
    ..Characterization of the FGFR1 and FGFR2 pathways in prostate cancer will contribute to the molecular understanding and early detection of patients that may experience rapid progression of metastases. ..
  101. Role and Mechanisms of Prolactin on HPA Axis Activation Following Stress
    James Janik; Fiscal Year: 2009
    ..Discovering the neuroendocrine mechanisms regulating the stress response will lead to improved treatments of stress-related disorders, and positively impact human health. ..