southern blotting

Summary

Summary: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

Top Publications

  1. pmc Small evolutionarily conserved RNA, resembling C/D box small nucleolar RNA, is transcribed from PWCR1, a novel imprinted gene in the Prader-Willi deletion region, which Is highly expressed in brain
    T de los Santos
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Am J Hum Genet 67:1067-82. 2000
  2. ncbi Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation
    Daniel J Weisenberger
    Urologic Cancer Research Laboratory, Department of Biochemistry and Molecular Biology, University of Southern California Norris Comprehensive Cancer Center, Keck School of Medicine, 1441 Eastlake Avenue, Los Angeles, CA 90089 9181, USA
    Mol Cancer Res 2:62-72. 2004
  3. pmc Evidence that plasmid-borne botulinum neurotoxin type B genes are widespread among Clostridium botulinum serotype B strains
    Giovanna Franciosa
    Department of Food Safety and Veterinary Public Health, Unit of Microorganisms and Food Technologies, Istituto Superiore di Sanita, Rome, Italy
    PLoS ONE 4:e4829. 2009
  4. pmc The bHLH factor Olig3 coordinates the specification of dorsal neurons in the spinal cord
    Thomas Muller
    Max Delbruck Center for Molecular Medicine, 13122 Berlin, Germany
    Genes Dev 19:733-43. 2005
  5. ncbi Possible horizontal transfer of a transposable element from host to parasitoid
    M Yoshiyama
    Laboratory of Applied Zoology, Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki, Japan
    Mol Biol Evol 18:1952-8. 2001
  6. pmc Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts
    H Daniell
    Department of Molecular Biology and Microbiology and Center for Discovery of Drugs and Diagnostics, University of Central Florida, 12722 Research Parkway, Orlando, FL 32826 3227, USA
    J Mol Biol 311:1001-9. 2001
  7. pmc Analysis of repetitive element DNA methylation by MethyLight
    Daniel J Weisenberger
    Department of Surgery, Keck School of Medicine, USC Norris Comprehensive Cancer Center, University of Southern California Los Angeles, CA, USA
    Nucleic Acids Res 33:6823-36. 2005
  8. pmc Insights into the role of histone H3 and histone H4 core modifiable residues in Saccharomyces cerevisiae
    Edel M Hyland
    High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Mol Cell Biol 25:10060-70. 2005
  9. ncbi Telomere length in small-for-gestational-age babies
    A Akkad
    Fetal Growth and Development Research Group, Reproductive Science Section, Department of Cancer Studies and Molecular Medicine, Univerity of Leicester, Leicester, UK
    BJOG 113:318-23. 2006
  10. pmc Allopolyploidy-induced rapid genome evolution in the wheat (Aegilops-Triticum) group
    H Ozkan
    Department of Plant Sciences, The Weizmann Institute of Science, Rehovot 72100, Israel
    Plant Cell 13:1735-47. 2001

Detail Information

Publications296 found, 100 shown here

  1. pmc Small evolutionarily conserved RNA, resembling C/D box small nucleolar RNA, is transcribed from PWCR1, a novel imprinted gene in the Prader-Willi deletion region, which Is highly expressed in brain
    T de los Santos
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Am J Hum Genet 67:1067-82. 2000
    ..On a multispecies Southern blot, hybridization to an HMCR probe encoding the putative snoRNA is limited to mammals...
  2. ncbi Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation
    Daniel J Weisenberger
    Urologic Cancer Research Laboratory, Department of Biochemistry and Molecular Biology, University of Southern California Norris Comprehensive Cancer Center, Keck School of Medicine, 1441 Eastlake Avenue, Los Angeles, CA 90089 9181, USA
    Mol Cancer Res 2:62-72. 2004
    ..Methylation analyses of immunodeficiency, chromosomal instabilities, and facial abnormalities cells revealed that an Alu repeat sequence was highly methylated, suggesting that Alu sequences are not DNMT3b targets...
  3. pmc Evidence that plasmid-borne botulinum neurotoxin type B genes are widespread among Clostridium botulinum serotype B strains
    Giovanna Franciosa
    Department of Food Safety and Veterinary Public Health, Unit of Microorganisms and Food Technologies, Istituto Superiore di Sanita, Rome, Italy
    PLoS ONE 4:e4829. 2009
    ..botulinum type B, Ab, and A(B), and whether plasmid carriage is bont/B subtype-related...
  4. pmc The bHLH factor Olig3 coordinates the specification of dorsal neurons in the spinal cord
    Thomas Muller
    Max Delbruck Center for Molecular Medicine, 13122 Berlin, Germany
    Genes Dev 19:733-43. 2005
    ..We conclude that Olig3 expression distinguishes the two major classes of progenitors in the dorsal spinal cord and determines the distinct specification program of class A neurons...
  5. ncbi Possible horizontal transfer of a transposable element from host to parasitoid
    M Yoshiyama
    Laboratory of Applied Zoology, Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki, Japan
    Mol Biol Evol 18:1952-8. 2001
    ..honmai were 97.6% identical in DNA sequence. This high similarity suggests a recent horizontal transfer, probably from the moth host to the wasp parasitoid, facilitated by the intimacy of the host-parasitoid relationship...
  6. pmc Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts
    H Daniell
    Department of Molecular Biology and Microbiology and Center for Discovery of Drugs and Diagnostics, University of Central Florida, 12722 Research Parkway, Orlando, FL 32826 3227, USA
    J Mol Biol 311:1001-9. 2001
    ....
  7. pmc Analysis of repetitive element DNA methylation by MethyLight
    Daniel J Weisenberger
    Department of Surgery, Keck School of Medicine, USC Norris Comprehensive Cancer Center, University of Southern California Los Angeles, CA, USA
    Nucleic Acids Res 33:6823-36. 2005
    ....
  8. pmc Insights into the role of histone H3 and histone H4 core modifiable residues in Saccharomyces cerevisiae
    Edel M Hyland
    High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Mol Cell Biol 25:10060-70. 2005
    ..Finally, we allude to the possible molecular mechanisms underlying the effects of these modifications...
  9. ncbi Telomere length in small-for-gestational-age babies
    A Akkad
    Fetal Growth and Development Research Group, Reproductive Science Section, Department of Cancer Studies and Molecular Medicine, Univerity of Leicester, Leicester, UK
    BJOG 113:318-23. 2006
    ..Short telomeres are associated with adult cardiovascular disease. Our aim was to determine whether small-for-gestational-age (SGA) newborns have shortened telomeres compared with appropriately grown controls...
  10. pmc Allopolyploidy-induced rapid genome evolution in the wheat (Aegilops-Triticum) group
    H Ozkan
    Department of Plant Sciences, The Weizmann Institute of Science, Rehovot 72100, Israel
    Plant Cell 13:1735-47. 2001
    ..In our view, this rapid genome adjustment may have contributed to the successful establishment of newly formed allopolyploids as new species...
  11. ncbi A chromosomal ars operon homologue of Pseudomonas aeruginosa confers increased resistance to arsenic and antimony in Escherichia coli
    J Cai
    Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada
    Microbiology 144:2705-13. 1998
    ..DNA sequences homologous with this operon were detected in some, but not all, species of the genus Pseudomonas, suggesting that its conservation follows their taxonomic-based evolution...
  12. ncbi Comparative gene analysis of Biomphalaria glabrata hemocytes pre- and post-exposure to miracidia of Schistosoma mansoni
    Nithya Raghavan
    Biomedical Research Institute, Rockville, MD 20852, USA
    Mol Biochem Parasitol 126:181-91. 2003
    ..However, identification of this and other transcripts from these cells by the EST approach provides a useful resource towards elucidating the molecular basis of resistance/susceptibility in this snail-host parasite relationship...
  13. ncbi Transformation of Stomoxys calcitrans with a Hermes gene vector
    D A O'Brochta
    Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park, MD 20742 4450, USA
    Insect Mol Biol 9:531-8. 2000
    ..melanogaster should be considered for widespread, constitutive expression. All transgenic lines contained multiple (2-4) integrated Hermes elements. Hermes integration events occurred through a canonical cut-and-paste mechanism...
  14. pmc Ancient mitochondrial haplotypes and evidence for intragenic recombination in a gynodioecious plant
    Thomas Stadler
    Department of Biology, Jordan Hall, 1001 East Third Street, Indiana University, Bloomington, IN 47405, USA
    Proc Natl Acad Sci U S A 99:11730-5. 2002
    ..Heteroplasmy and recombination between divergent haplotypes imply unexplored consequences for the evolutionary dynamics of gynodioecy, a widespread plant breeding system...
  15. pmc B lymphocyte-specific, Cre-mediated mutagenesis in mice
    R C Rickert
    Institute for Genetics, University of Cologne, Weyertal 121, 50931 Cologne, Germany
    Nucleic Acids Res 25:1317-8. 1997
    ..In a model system involving the cross of CD19-Cre mice with mice bearing a loxP -flanked substrate, we find a deletion efficiency of 75-80% in bone marrow-derived pre-B cells that increases to 90-95% in splenic B cells...
  16. ncbi Development of a new PCR assay to identify Brucella abortus biovars 5, 6 and 9 and the new subgroup 3b of biovar 3
    Alain A Ocampo-Sosa
    Departamento de Biologia Molecular, Facultad de Medicina Universidad de Cantabria, Unidad Asociada al CIB, CSIC, c Cardenal Herrera Oria s n, 39011 Santander, Spain
    Vet Microbiol 110:41-51. 2005
    ..Addition of this primer to the AMOS-ERY PCR primer cocktail allows the positive distinction of B. abortus biovars 3b, 5, 6 and 9 from the rest of Brucella species and biovars...
  17. pmc Doubling genome size without polyploidization: dynamics of retrotransposition-driven genomic expansions in Oryza australiensis, a wild relative of rice
    Benoit Piegu
    Laboratoire Génome et Développement des Plantes, UMR 5096 CNRS IRD, Universite de Perpignan, Perpignan 66860, France
    Genome Res 16:1262-9. 2006
    ..This provides direct evidence of retrotransposon-mediated variation of genome size within a plant genus...
  18. ncbi Vector fragmentation: characterizing vector integrity in transfected clones by Southern blotting
    Say Kong Ng
    Bioprocessing Technology Institute, Agency for Science, Technology and Research A STAR, Centros, Singapore 138668
    Biotechnol Prog 26:11-20. 2010
    ..Here, we elucidated the vector integration patterns of 40 transfected single-cell clones by Southern blotting and showed that vector fragmentation occurs at a significant level in our experiment...
  19. pmc A parasitic phase-specific adhesin of Coccidioides immitis contributes to the virulence of this respiratory Fungal pathogen
    Chiung Yu Hung
    Department of Microbiology and Immunology, Medical College of Ohio, Toledo, Ohio 43614 5086, USA
    Infect Immun 70:3443-56. 2002
    ..The parasitic cell surface glycoprotein encoded by the SOWgp gene appears to function as an adhesin and contributes to the virulence of C. immitis...
  20. ncbi Ongoing loss of the tirant transposable element in natural populations of Drosophila simulans
    Marie Fablet
    UMR CNRS 5558, Biometrie et Biologie Evolutive, Universite Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, France
    Gene 375:54-62. 2006
    ..simulans populations reflects an ancient invasion of D. simulans by tirant copies followed by the loss of active copies from most populations, apart from the African ones, suggesting that this loss is still ongoing in this species...
  21. ncbi Polyadenylated transcripts containing random gene fragments are expressed in dinoflagellate mitochondria
    Hélène Chaput
    Departement de sciences biologiques, Universite de Montreal, Canada
    Protist 153:111-22. 2002
    ..Gene fragments or multiple copies of the cox3 and cob sequences may be abundant in the mitochondrial genome since Southern blots, using these sequences separately as probes, show multiple restriction fragments with several enzymes...
  22. pmc The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection
    D A Haake
    Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California 90073, USA
    Infect Immun 68:2276-85. 2000
    ..The sequence and expression of LipL32 is highly conserved among pathogenic Leptospira species. These findings indicate that LipL32 may be important in the pathogenesis, diagnosis, and prevention of leptospirosis...
  23. ncbi Southern blotting
    Ed Southern
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Nat Protoc 1:518-25. 2006
    ..This protocol can be completed in 1-5 d and is inexpensive to carry out, as it requires only basic laboratory equipment...
  24. ncbi The necessity of mitochondrial genome DNA for normal development of Dictyostelium cells
    Junji Chida
    Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980 8578, Japan
    J Cell Sci 117:3141-52. 2004
    ..Taken together, these results suggest that mtDNA might have important multiple functions in a variety of cellular processes during Dictyostelium development...
  25. ncbi Identification and characterization of a spinal muscular atrophy-determining gene
    S Lefebvre
    Unite de Recherches sur les Handicaps Genetiques de l Enfant, Institut National de la Sante et de la Recherche Medicale, Institut Necker, Hopital des Enfants Malades, Paris, France
    Cell 80:155-65. 1995
    ..These data suggest that this gene, termed the survival motor neuron (SMN) gene, is an SMA-determining gene...
  26. pmc The length of telomeric G-rich strand 3'-overhang measured by oligonucleotide ligation assay
    G Cimino-Reale
    Istituto di Neurobiologia e Medicina Molecolare, CNR, Viale Marx 15-43, I-00137, Roma, Italy
    Nucleic Acids Res 29:E35. 2001
    ..Our protocol provides a simple and sensitive method for measuring the length of naturally occurring unpaired repeated DNA...
  27. ncbi The stage-regulated expression of Leishmania mexicana CPB cysteine proteases is mediated by an intercistronic sequence element
    D R Brooks
    Wellcome Centre for Molecular Parasitology, University of Glasgow, The Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, United Kingdom
    J Biol Chem 276:47061-9. 2001
    ..This is the first well defined cis-regulatory element implicated in post-transcriptional stage-specific gene expression in Leishmania...
  28. ncbi Efficiency and stability of high molecular weight DNA transformation: an analysis in tomato
    A Frary
    Plant Science Center, Center for Advanced Technology Biotechnology, Cornell University, Ithaca, NY 14853, USA
    Transgenic Res 10:121-32. 2001
    ..Expression of a selectable marker gene within the T-DNA was also examined through several generations and no gene silencing was observed. Thus, the BIBAC is a useful system for transfer of large DNA fragments into the plant genome...
  29. pmc "Parahomologous" gene targeting in Drosophila cells: an efficient, homology-dependent pathway of illegitimate recombination near a target site
    L Cherbas
    Department of Biology, Indiana University, Bloomington 47405, USA
    Genetics 145:349-58. 1997
    ..The efficiency of this process, its homology dependence, and the clustering of the products define a novel transformation pathway that we call "parahomologous targeting."..
  30. ncbi Characterization of the murine mu opioid receptor gene
    D L Kaufman
    Department of Molecular and Medical Pharmacology, University of California, Los Angeles 90024, USA
    J Biol Chem 270:15877-83. 1995
    ....
  31. ncbi A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases
    K T Arndt
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142
    Cell 56:527-37. 1989
    ..sit4 mutations in combination with suppressor mutations in RPB1 or RPB2 (sit1, sit4 or sit2, sit4) are lethal, suggesting an interaction between SIT4 and RNA polymerase II...
  32. ncbi Substrate specificity and expression profile of amino acid transporters (AAPs) in Arabidopsis
    W N Fischer
    Institut fur Genbiologische Forschung, Berlin, Germany
    J Biol Chem 270:16315-20. 1995
    ..The specific distribution in the plant and the different substrate specificities of AAP transporters may indicate that tissues differ both qualitatively and quantitatively regarding import or export of amino acids...
  33. ncbi The gene PPG encodes a novel yeast protein phosphatase involved in glycogen accumulation
    F Posas
    Departament de Bioquimica i Biologia Molecular, Facultat de Veterinaria, Universitat Autonoma de Barcelona, Bellaterra, Spain
    J Biol Chem 268:1349-54. 1993
    ....
  34. ncbi Genome expansion in three hybrid sunflower species is associated with retrotransposon proliferation
    Mark C Ungerer
    Curr Biol 16:R872-3. 2006
  35. pmc Novel mutations and deletions of the KIT (steel factor receptor) gene in human piebaldism
    K Ezoe
    Department of Medical Genetics, University of Wisconsin, Madison 53706
    Am J Hum Genet 56:58-66. 1995
    ..Thus, most patients with typical piebaldism appear to have abnormalities of the KIT gene...
  36. pmc Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules
    G N Wheeler
    National Institute for Medical Research, Mill Hill, London, United Kingdom
    Proc Natl Acad Sci U S A 88:4796-800. 1991
    ..As in the cadherins, the extracellular domain contains possible Ca2(+)-binding sequences and a potential protease processing site. The cell adhesion recognition region (His-Ala-Val) of the cadherins is modified to Arg-Ala-Leu...
  37. pmc Genetic characterization of Clostridium botulinum associated with type B infant botulism in Japan
    Kaoru Umeda
    Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8 34 Tojo cho, Tennoji ku, Osaka 543 0026, Japan
    J Clin Microbiol 47:2720-8. 2009
    ..We established a multiplex PCR assay for BoNT/B subtyping which will be useful for epidemiological studies of type B strains and the infectious diseases that they cause...
  38. ncbi Differential expression of the "B" subunit of the vacuolar H(+)-ATPase in bovine tissues
    K Puopolo
    Department of Physiology and Molecular Biology, Tufts University School of Medicine, Boston, Massachusetts 02111
    J Biol Chem 267:3696-706. 1992
    ..2- or 2.0-kb message on Northern blot. Southern blot analysis of bovine genomic DNA with probes derived from both isolated cDNAs indicates that the bovine B subunit is encoded by a multigene family...
  39. ncbi Neonatal lethality and lymphopenia in mice with a homozygous disruption of the c-abl proto-oncogene
    V L Tybulewicz
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    Cell 65:1153-63. 1991
    ..Most mice homozygous for the c-abl mutation became runted and died 1 to 2 weeks after birth. In addition, many showed thymic and splenic atrophy and a T and B cell lymphopenia...
  40. pmc Coordinate hypermethylation at specific genes in prostate carcinoma precedes LINE-1 hypomethylation
    A R Florl
    Department of Urology, Heinrich Heine University Duesseldorf, Moorenstr 5, 40225 Duesseldorf, Germany
    Br J Cancer 91:985-94. 2004
    ..Hypermethylation allows detection of >82% of PCas. PCa may fall into three classes, that is, with few DNA methylation changes, with frequent hypermethylation, or with additional LINE-1 hypomethylation...
  41. ncbi A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae
    L Cao
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Cell 61:1089-101. 1990
    ..More importantly, these observations begin to define a pathway for the physical changes in DNA that lead to recombination and to define the roles of meiotic recombination functions in that pathway...
  42. ncbi Proviral status of HTLV-1 integrated into the host genomic DNA of adult T-cell leukemia cells
    S Kamihira
    Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1 7 1 Sakamoto, Nagasaki City, Japan 852 8501
    Clin Lab Haematol 27:235-41. 2005
    ..These results suggest that analysis of the proviral status provides useful diagnostic and virologic-oncological information about ATL and HTLV-1 pathology, especially the important role of pX gene in tumorigenesis...
  43. pmc DNA amplification by breakage/fusion/bridge cycles initiated by spontaneous telomere loss in a human cancer cell line
    Anthony W I Lo
    Radiation Oncology Research Laboratory, University of California, 1855 Folsom Street, MCB 200, San Francisco, CA 94103, USA
    Neoplasia 4:531-8. 2002
    ..The amplification of subtelomeric DNA on the marker chromosome provides conclusive evidence that B/F/B cycles initiated by spontaneous telomere loss are a mechanism for gene amplification in human cancer cells...
  44. pmc Transcriptional network of multiple capsule and melanin genes governed by the Cryptococcus neoformans cyclic AMP cascade
    Read Pukkila-Worley
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Eukaryot Cell 4:190-201. 2005
    ..These studies provide mechanistic insights into the regulation of capsule and melanin production by the C. neoformans cAMP pathway and demonstrate that multiple laccases contribute to C. neoformans melanin production and pathogenesis...
  45. ncbi Decreased expression of specific genes in yeast cells lacking histone H1
    K Hellauer
    Department of Medicine, Royal Victoria Hospital, McGill University, , , Canada
    J Biol Chem 276:13587-92. 2001
    ..A similar effect was observed with an integrated lacZ reporter. Thus, our data demonstrate that removal of yeast histone H1 only results in decreased gene expression...
  46. ncbi PpRT1: the first complete gypsy-like retrotransposon isolated in Pinus pinaster
    Margarida Rocheta
    Forest Biotech Lab, Instituto de Biologia Experimental e Tecnológica IBET ITQB, Quinta do Marques, 2784 505 Oeiras, Portugal
    Planta 225:551-62. 2007
    ....
  47. ncbi A functional antigen in a practical crop: LT-B producing maize protects mice against Escherichia coli heat labile enterotoxin (LT) and cholera toxin (CT)
    Rachel Chikwamba
    Department ofAgronomy, Iowa State University, Ames 50011 1010, USA
    Transgenic Res 11:479-93. 2002
    ..We have demonstrated that maize, a major food and feed ingredient, can be efficiently transformed to produce, accumulate, and store a fully assembled and functional candidate vaccine antigen...
  48. ncbi Foxp1 is an essential transcriptional regulator of B cell development
    Hui Hu
    Department of Pathology, Harvard Medical School, CBR Institute for Biomedical Research, Boston, Massachusetts 02115, USA
    Nat Immunol 7:819-26. 2006
    ..Our results identify Foxp1 as an essential participant in the transcriptional regulatory network of B lymphopoiesis...
  49. ncbi Diversification of Ig superfamily genes in an invertebrate
    Si Ming Zhang
    Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA
    Science 305:251-4. 2004
    ..Diverse FREP3 transcripts are also produced. We hypothesize a mechanism present in snails that is capable of diversifying molecules involved in internal defense...
  50. pmc Identification of novel serine/threonine protein phosphatases in Trypanosoma cruzi: a potential role in control of cytokinesis and morphology
    G A Orr
    Departments of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Infect Immun 68:1350-8. 2000
    ..cruzi epimastigotes and metacyclic trypomastigote extracts. These inhibitor studies suggest that in T. cruzi, type 1 protein phosphatases are important for the completion of cell division and for the maintenance of cell shape...
  51. ncbi RNA silencing in the phytopathogenic fungus Magnaporthe oryzae
    Naoki Kadotani
    Laboratory of Plant Pathology, Kobe University, 1 1 Rokkodaicho, Nada, 657 8501 Kobe, Japan
    Mol Plant Microbe Interact 16:769-76. 2003
    ..Overall, these results indicate that RNA silencing operates in M. oryzae, which gives us a new tool for genome-wide gene analysis in this fungus...
  52. ncbi A two-step protocol for the detection of rearrangements at the AZFc region on the human Y chromosome
    Y W Lin
    Graduate Institute of Life Sciences, National Defense Medical Center and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
    Mol Hum Reprod 12:347-51. 2006
    ..It offers a simple and reliable way to screen large populations for AZFc rearrangements and study their effects on male fertility...
  53. pmc Comparison of virulence plasmids among Clostridium perfringens type E isolates
    Jihong Li
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Infect Immun 75:1811-9. 2007
    ..Further iap/ibp plasmid diversity occurred when either the iap/ibp genes later remobilized and inserted onto other conjugative plasmids or some iap/ibp plasmids acquired additional DNA sequences...
  54. pmc Disruption of the PfPK7 gene impairs schizogony and sporogony in the human malaria parasite Plasmodium falciparum
    Dominique Dorin-Semblat
    INSERM U609, Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow G12 8TA, Scotland, United Kingdom
    Eukaryot Cell 7:279-85. 2008
    ..Hence, PfPK7 is involved in a pathway that regulates parasite proliferation and development...
  55. pmc A targeted gene knockout in Drosophila
    Y S Rong
    Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
    Genetics 157:1307-12. 2001
    ..The results indicate that this method of homologous recombination is likely to be generally useful for Drosophila gene targeting...
  56. pmc TAC1, transcriptional activator of CDR genes, is a new transcription factor involved in the regulation of Candida albicans ABC transporters CDR1 and CDR2
    Alix T Coste
    Institute of Microbiology, University Hospital Lausanne, Rue du Bugnon 48, CH 1011 Lausanne, Switzerland
    Eukaryot Cell 3:1639-52. 2004
    ..In conclusion, TAC1 appears to be the first C. albicans transcription factor involved in the control of genes mediating antifungal resistance...
  57. ncbi Mutagenesis of Streptococcus equi and Streptococcus suis by transposon Tn917
    J D Slater
    Department of Clinical Veterinary Medicine, Centre for Veterinary Science, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
    Vet Microbiol 93:197-206. 2003
    ..suis and S. equi mutants was analysed by Southern blotting. However, when analysis of 60 S. equi mutants was carried out using the S...
  58. pmc Virulence plasmid diversity in Clostridium perfringens type D isolates
    Sameera Sayeed
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Infect Immun 75:2391-8. 2007
    ..b>Southern blotting of pulsed-field gels showed that the etx gene of type D isolates can be present on at least five different ..
  59. ncbi Chromosomal engineering of Clostridium perfringens using group II introns
    Phalguni Gupta
    Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA
    Methods Mol Biol 435:217-28. 2008
    ..perfringens genes. The usefulness of this system has now been demonstrated by specifically inactivating four different C. perfringens toxin genes...
  60. pmc Agrobacterium tumefaciens integrates transfer DNA into single chromosomal sites of dimorphic fungi and yields homokaryotic progeny from multinucleate yeast
    Thomas D Sullivan
    Department of Pediatrics, University of Wisconsin School of Medicine, Madison, Wisconsin, USA
    Eukaryot Cell 1:895-905. 2002
    ..Taken together, the results demonstrate that A. tumefaciens efficiently transfers DNA into B. dermatitidis and H. capsulatum and has the properties necessary for use as an insertional mutagen in these fungi...
  61. pmc Genetic characterization of type A enterotoxigenic Clostridium perfringens strains
    Agi Deguchi
    Department of Microbiology, Wakayama Medical University School of Medicine, Wakayama, Japan
    PLoS ONE 4:e5598. 2009
    ..perfringens strains...
  62. pmc A novel streptococcal surface protease promotes virulence, resistance to opsonophagocytosis, and cleavage of human fibrinogen
    Theresa O Harris
    Division of Infectious Disease, Children s Hospital and Regional Medical Center, and University of Washington, Seattle, Washington, USA
    J Clin Invest 111:61-70. 2003
    ..gene was present among representative clinical isolates from all nine capsular serotypes, as revealed by Southern blotting. A cspA(-) mutant was ten times less virulent in a neonatal rat sepsis model of GBS infections, as measured ..
  63. ncbi Genetic and epigenetic changes of rDNA in a synthetic allotetraploid, Aegilops sharonensis x Ae. umbellulata
    A B Shcherban
    Institute of Cytology and Genetics, Lavrentiev ave 10, Novosibirsk, 630090, Russia
    Genome 51:261-71. 2008
    ..umbellulata-derived rDNA units. The fast uniparental elimination of rDNA observed in the synthetic allopolyploid agrees well with patterns observed previously in natural wheat allotetraploids...
  64. pmc Prevalence of enterotoxigenic Clostridium perfringens Isolates in Pittsburgh (Pennsylvania) area soils and home kitchens
    Jihong Li
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Appl Environ Microbiol 73:7218-24. 2007
    ....
  65. ncbi Rates of movement and distribution of transposable elements in Drosophila melanogaster: in situ hybridization vs Southern blotting data
    X Maside
    Institute for Cell, Animal and Population Biology, University of Edinburgh, Ashworth Laboratories, Edinburgh EH9 3JT, UK
    Genet Res 78:121-36. 2001
    ..b>Southern blotting was used to screen a large number of samples from both genetic backgrounds for TEs...
  66. ncbi Deletion of the C4-CYP21 repeat module leading to the formation of a chimeric CYP21P/CYP21 gene in a 9.3-kb fragment as a cause of steroid 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Ltd, Yuan Shan Research Institute, No 326 Yuan Shan Rd, Sec 2, Yuan Shan, Ilan 264, Taiwan, Republic of China
    Clin Chem 49:319-22. 2003
  67. ncbi Enhancer mutations of Akv murine leukemia virus inhibit the induction of mature B-cell lymphomas and shift disease specificity towards the more differentiated plasma cell stage
    Karina Dalsgaard Sørensen
    Department of Molecular Biology, University of Aarhus, C F Møllers Alle, Bldg 130, DK 8000 Aarhus C, Denmark
    Virology 362:179-91. 2007
    ..Our results indicate that enhancer mutations weaken the ability of Akv to induce mature B-cell lymphomas prior to the plasma cell stage, whereas development of plasma cell proliferations is less dependent of viral enhancer strength...
  68. pmc Organization of the plasmid cpe Locus in Clostridium perfringens type A isolates
    Kazuaki Miyamoto
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pennsylvania 15261, USA
    Infect Immun 70:4261-72. 2002
    ..perfringens plasmid. The similarity of the plasmid cpe locus in many type A isolates is consistent with horizontal transfer of a common cpe plasmid among C. perfringens type A strains...
  69. ncbi Telomere shortening of peripheral blood mononuclear cells in coronary disease patients with metabolic disorders
    Nobuya Obana
    Department of Gastroenterology, Tohoku University Postgraduate School of Medicine, Sendai
    Intern Med 42:150-3. 2003
    ..Telomere shortening is correlated with cell turnover and aging, but it has been recently suggested to occur not only by aging but by several biochemical factors of metabolic disorders predisposing to atherosclerosis...
  70. pmc Plasmid encoded neurotoxin genes in Clostridium botulinum serotype A subtypes
    Kristin M Marshall
    Department of Bacteriology, University of Wisconsin, 1925 Willow Drive, Madison, WI 53706, USA
    Biochem Biophys Res Commun 361:49-54. 2007
    ..The finding of BoNT type A and B genes on extrachromosomal elements has important implications for the evolution of neurotoxigenicity in clostridia including the origin, expression, and lateral transfer of botulinum neurotoxin genes...
  71. ncbi T-DNA transfer and integration in the ectomycorrhizal fungus Suillus bovinus using hygromycin B as a selectable marker
    Mubashir Hanif
    Department of Biosciences, University of Helsinki, Finland
    Curr Genet 41:183-8. 2002
    ..bovinus genome. A. tumeficiens transformation should make possible the development of tagged mutagenesis and targeted gene disruption technology for S. bovinus...
  72. pmc Sequencing and diversity analyses reveal extensive similarities between some epsilon-toxin-encoding plasmids and the pCPF5603 Clostridium perfringens enterotoxin plasmid
    Kazuaki Miyamoto
    Department of Microbiology, Wakayama Medical University School of Medicine, Wakayama, Japan
    J Bacteriol 190:7178-88. 2008
    ..These results suggest that pCPF5603 and pCP8533etx evolved from insertion of mobile genetic elements carrying enterotoxin or etx genes, respectively, onto a common progenitor plasmid...
  73. ncbi Erosion of the telomeric single-strand overhang at replicative senescence
    Sheila A Stewart
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Nat Genet 33:492-6. 2003
    ..We thus provide evidence for a specific molecular alteration in telomere structure at senescence and suggest that this change, rather than overall telomere length, serves to trigger this state...
  74. pmc Regulation of genome stability by TEL1 and MEC1, yeast homologs of the mammalian ATM and ATR genes
    Rolf J Craven
    Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Genetics 161:493-507. 2002
    ..Similar chromosome rearrangements have been detected in mammalian cells with mutations in ATM (related to TEL1) and ATR (related to MEC1) and in mammalian cells that approach cell crisis...
  75. pmc Two CD1 genes map to the chicken MHC, indicating that CD1 genes are ancient and likely to have been present in the primordial MHC
    Jan Salomonsen
    Department of Pathobiology, Laboratory of Immunology, Royal Veterinary and Agricultural University, Stigbøjlen 7, DK 1870 Frederiksberg C, Denmark
    Proc Natl Acad Sci U S A 102:8668-73. 2005
    ..Flow cytometry confirms expression from one gene on B cells. Based on Southern blotting and cloning, only two such CD1 genes are detected, located approximately 800 nucleotides apart and in the ..
  76. ncbi The dingo non-long terminal repeat retrotransposons from the genome of the hookworm, Ancylostoma caninum
    Thewarach Laha
    Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
    Exp Parasitol 113:142-53. 2006
    ..caninum. The dingo elements are the first retrotransposons to be characterized from a hookworm genome...
  77. ncbi Detection and quantitation of HPV DNA replication by Southern blotting and real-time PCR
    Iain M Morgan
    Institute of Comparative Medicine, University of Glasgow, Scotland
    Methods Mol Med 119:349-62. 2005
    ..introduction into the mechanism of DNA replication by the E1 and E2 proteins and describes the traditional Southern blotting technique that is used to monitor E1- and E2-mediated DNA replication...
  78. pmc Contractions of D4Z4 on 4qB subtelomeres do not cause facioscapulohumeral muscular dystrophy
    Richard J F L Lemmers
    Leiden University Medical Center, Center for Human and Clinical Genetics, Department of Human Genetics, Leiden, The Netherlands
    Am J Hum Genet 75:1124-30. 2004
    ..Thus, in addition to a contraction of D4Z4, additional cis-acting elements on 4qA may be required for the development of FSHD. Alternatively, 4qB subtelomeres may contain elements that prevent FSHD pathogenesis...
  79. ncbi Extensive alterations in DNA methylation and transcription in rice caused by introgression from Zizania latifolia
    Zhenlan Liu
    Laboratory of Molecular Epigenetics, Northeast Normal University, Changchun 130024, China
    Plant Mol Biol 54:571-82. 2004
    ..Thus, we have demonstrated that alien DNA introgression into a plant genome can induce extensive alterations in DNA methylation and transcription of both cellular genes and TE-related DNA segments in a genotype-independent manner...
  80. pmc The genome sequence of Clostridium botulinum type C neurotoxin-converting phage and the molecular mechanisms of unstable lysogeny
    Yoshihiko Sakaguchi
    Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Proc Natl Acad Sci U S A 102:17472-7. 2005
    ....
  81. ncbi MHC RFLP analyses in channel catfish full-sibling families: identification of the role of MHC molecules in spontaneous allogeneic cytotoxic responses
    Sylvie M A Quiniou
    Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA
    Dev Comp Immunol 29:457-67. 2005
    ....
  82. pmc The Sinbad retrotransposon from the genome of the human blood fluke, Schistosoma mansoni, and the distribution of related Pao-like elements
    Claudia S Copeland
    Department of Tropical Medicine, Tulane University Health Sciences Center, New Orleans, USA
    BMC Evol Biol 5:20. 2005
    ..It is becoming apparent that numerous LTR retrotransposons and other mobile genetic elements have colonized the genome of the human blood fluke, Schistosoma mansoni...
  83. pmc Rapid directional shift of mitochondrial DNA heteroplasmy in animal tissues by a mitochondrially targeted restriction endonuclease
    Maria Pilar Bayona-Bafaluy
    Department of Neurology and Cell Biologyalysis, University of Miami Miller School of Medicine, Miami, FL 33136, USA
    Proc Natl Acad Sci U S A 102:14392-7. 2005
    ..This strategy could prevent disease onset or reverse clinical symptoms in patients harboring certain heteroplasmic pathogenic mutations in mtDNA...
  84. ncbi Identification of an active transposon in intact rice plants
    Kenji Fujino
    Hokkaido Green Bio Institute, Naganuma, Hokkaido, 0691317, Japan
    Mol Genet Genomics 273:150-7. 2005
    ..Because the transposons are active in intact rice plants, they should be useful tools for tagging genes in studies of functional genomics...
  85. ncbi Screening for mutations in haemophilia A patients by multiplex PCR-SSCP, Southern blotting and RNA analysis: the detection of a genetic abnormality in the factor VIII gene in 30 out of 35 patients
    W C Pieneman
    Department of Haematology, University Hospital, Leiden, The Netherlands
    Br J Haematol 90:442-9. 1995
    ..molecular characterization of mutations in haemophilia A patients in this study was carried out by PCR-SSCP, Southern blotting, and reverse transcribed-PCR...
  86. pmc Molecular breakpoint cloning and gene expression studies of a novel translocation t(4;15)(q27;q11.2) associated with Prader-Willi syndrome
    Birgitt Schule
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    BMC Med Genet 6:18. 2005
    ..To further dissect the PWS phenotype and define the minimal critical region for PWS features, we have studied a 22 year old male with a milder PWS phenotype and a de novo translocation t(4;15)(q27;q11.2)...
  87. ncbi Polymorphisms associated with the DAZ genes on the human Y chromosome
    Yi Wen Lin
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
    Genomics 86:431-8. 2005
    ..Additional polymorphisms identified within the DAZ repeat regions of the DAZ genes indicate that sister chromatid exchange plays a significant role in the genesis of deletions, duplications, and polymorphisms of the Y chromosome...
  88. ncbi Retrotransposon populations of Vicia species with varying genome size
    Pamela Hill
    Plant Research Unit, University of Dundee at SCRI, Invergowrie, Dundee, DD2 5DA, UK
    Mol Genet Genomics 273:371-81. 2005
    ..These data show that the three retrotransposon groups have proliferated to different extents in members of the Vicia genus and high proliferation has been associated with homogenisation of the retrotransposon population...
  89. ncbi Analysis of telomeric single-strand overhang length in human endometrial cancers
    Manabu Hashimoto
    Department of Obstetrics and Gynecology, Graduate School of Kanazawa University, Ishikawa, Japan
    FEBS Lett 579:2959-64. 2005
    ..These findings suggest that erosion of 3'-OH length, rather than overall telomere length, play roles in endometrial carcinogenesis. Furthermore, long 3'-OH may serve as a molecular marker for aggressive phenotype of tumors...
  90. pmc Characterization of two avian MHC-like genes reveals an ancient origin of the CD1 family
    Marcia M Miller
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Harvard Medical School, Smith 516C, 1 Jimmy Fund Way, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:8674-9. 2005
    ..The existence of CD1 genes in an avian species suggests that the origin of CD1 extends deep into the evolutionary history of terrestrial vertebrates...
  91. ncbi Telomere attrition in white blood cell correlating with cardiovascular damage
    Hidekatsu Nakashima
    Department of Clinical Laboratory Medicine, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan
    Hypertens Res 27:319-25. 2004
    ....
  92. ncbi Duplications of the functional CYP21A2 gene are primarily restricted to Q318X alleles: evidence for a founder effect
    S Kleinle
    Department of Internal Medicine III, Division of Clinical Endocrinology and Metabolism, Medical University of Vienna, A 1090 Vienna, Austria
    J Clin Endocrinol Metab 94:3954-8. 2009
    ....
  93. ncbi A group of related cDNAs encoding secreted proteins from Hessian fly [Mayetiola destructor (Say)] salivary glands
    M S Chen
    USDA ARS, Plant Science and Entomology Research Unit, Department of Entomology, Kansas State University, Manhattan, KS 66506, USA
    Insect Mol Biol 13:101-8. 2004
    ..Genomic analysis demonstrated that multiple copies of similar genes are clustered within a short region on chromosome 2A. This is the same arm in which two avirulence genes have been mapped...
  94. pmc Comparative genomics of Rickettsia prowazekii Madrid E and Breinl strains
    Hong Ge
    Rickettsial Diseases Department, Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    J Bacteriol 186:556-65. 2004
    ..The results of this first rickettsial DNA microarray may provide some important information for the elucidation of pathogenic mechanisms of R. prowazekii...
  95. ncbi A candidate autonomous version of the wheat MITE Hikkoshi is present in the rice genome
    M Saito
    Department of Crop Breeding, Tohoku National Agriculture Research Center, 4 Akahira, Shimo kuriyagawa, Morioka, Iwate, 020 0198, Japan
    Mol Genet Genomics 273:404-14. 2005
    ..The sequence information from the TPase of OsHikkoshi will thus be useful in isolating the autonomous element of the Hikkoshi system from wheat...
  96. pmc Characterization of SR3 reveals abundance of non-LTR retrotransposons of the RTE clade in the genome of the human blood fluke, Schistosoma mansoni
    Thewarach Laha
    Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
    BMC Genomics 6:154. 2005
    ..Of more than 12 recognized clades of non-LTR retrotransposons, only members of the CR1, RTE, and R2 clades have been reported from the schistosome genome...
  97. ncbi Undermethylation associated with retroelement activation and chromosome remodelling in an interspecific mammalian hybrid
    R J O'Neill
    Department of Genetics and Human Variation, La Trobe University, Bundoora, Victoria, Australia
    Nature 393:68-72. 1998
    ....
  98. ncbi Epstein-Barr virus integrates frequently into chromosome 4q, 2q, 1q and 7q of Burkitt's lymphoma cell line (Raji)
    Jianming Gao
    Cancer Research Institute, Xiang Ya School of Medicine, Central South University, Changsha, Hunan 410078, China
    J Virol Methods 136:193-9. 2006
    ..This study is the first comprehensive FISH analysis of EBV integration into the chromosomes of the Raji cell line. The findings support the notion that EBV integrates into the Raji cell genome non-randomly...
  99. pmc Horizontal transfer of segments of the 16S rRNA genes between species of the Streptococcus anginosus group
    Leo M Schouls
    Laboratory for Vaccine Preventable Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
    J Bacteriol 185:7241-6. 2003
    ..Such recombination may lead to the construction of incorrect phylogenetic trees based on the 16S rRNA genes...
  100. ncbi cDNA characterization and expression analysis of two arylphorin-like hexameric protein genes from the diamondback moth, Plutella xylostella (L.)
    Muhammad Ashfaq
    Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, Japan
    Arch Insect Biochem Physiol 64:175-85. 2007
    ..Expression of both genes in fourth instar larvae was suppressed after treatment with a JH-analog. Southern hybridization revealed the presence of multiple arylphorin genes in the genome...
  101. ncbi Molecular cloning and characterization of a mouse spermatogenesis-related ring finger gene znf230
    Weimin Qiu
    Department of Medical Genetics, West China Hospital, Sichuan University, Renminnanlu No 17, Section 3, 610041, Chengdu, PR China
    Biochem Biophys Res Commun 306:347-53. 2003
    ..Therefore, it is postulated that znf230 may function as a testis specific transcription factor during mouse spermatogenesis...

Research Grants62

  1. Gene therapy in the cornea
    RAJIV RAVINDRA MOHAN; Fiscal Year: 2012
    ..Targeted gene therapy delivered to the cornea with a simple topical technique offers promise to cure, treat and/or prevent vision loss by modulating excessive healing mechanisms that cause these blinding corneal disorders. ..
  2. Role of a new polyomavirus in Merkel cell carcinoma
    Patrick S Moore; Fiscal Year: 2013
    ..b>Southern blotting shows that MCPyV is integrated into MCC genome at different sites in a somatic and monoclonal pattern...
  3. The Role of Plasmids in Rickettsia Biology
    ULRIKE GERTRUD MUNDERLOH; Fiscal Year: 2013
    ..The results of this research may provide new tools to control Rickettsia infections of humans. ..
  4. Role of Calpastatin in mutant SOD1-mediated motor neuron disease
    Mala V Rao; Fiscal Year: 2010
    ..We will use mouse breeding techniques, Southern Blotting, polymerase chain reactions, immunoblotting, immunocytochemistry, electron microscopy and Rotarod tests to ..
  5. RECOMBINATION MECHANISMS IN YEAST CELL DIFFERENTIATION
    James E Haber; Fiscal Year: 2013
    ..Identification of the phospho-threonine target in the region around the DSB will be carried out, both by characterization of the FHA domain and by analysis of DSB-dependent proteins that associate with the FHA domain. ..
  6. FMR 1-SLS: Improving Fragile X diagnosis using amplification-free single locus ta
    STEPHEN WHITFIELD TURNER; Fiscal Year: 2013
    ..DNA fragment sizing methods are used today, either Southern Blotting or PCR followed by capillary electrophoresis, to categorize individuals as having normal, premutation or ..
  7. Enabling use of blood spot cards for accurate high-throughput Fragile X screening
    Gary J Latham; Fiscal Year: 2011
    ..The diagnostic tests detects DNA from a small drop of blood spotted on paper and will support early diagnosis which may be beneficial because of current and emerging options for early interventions. ..
  8. Osteosarcoma Genetics Using an Animal Model
    JENNIE L ROWELL; Fiscal Year: 2011
    ..100 CNVs will be validated by breakpoint-specific PCR where possible, as well as Southern blotting and Q-PCR. Genotyping will be done to detect CNV presence/absence using CNV allele specific PCR assays...
  9. Clostridium perfringens Type B-D Virulence Plasmids
    Francisco A Uzal; Fiscal Year: 2013
    ..abstract_text> ..
  10. Curcumin inhbits BPDE-induced damage by lowering the threshold of p53 activation
    ERICA NICOLE ROGERS; Fiscal Year: 2011
    ..In addition, the results from this project will benefit the public by providing easy and inexpensive alternative methods in preventing initial and continuing signs of lung cancer. ..
  11. Hematopoietic Reconstitution Using Ex Vivo Expanded Cord Blood CD34+ Cells
    Gerald J Spangrude; Fiscal Year: 2012
    ..the level of engraftment for each test groups will be evaluated using immunophenotyping/flow cytometry and Southern blotting (if necessary)...
  12. Leukocyte Telomere Length and Cardiovascular Disease in Jackson Heart Study
    JAMES GRAHAM WILSON; Fiscal Year: 2013
    ..Elucidating the LTL-CVD links in AfAs will provide mechanistic insight into pathways that promote atherosclerosis and LVH, as well as provide new diagnostic tools to identify susceptibility to CVD before its overt manifestations. ..
  13. CHARACTERIZATION OF SFHR MODIFICATION OF GENOMIC HPRT
    Dieter C Gruenert; Fiscal Year: 2010
    ..Characterization and optimization of SFHR-mediated modification will be a significant step in the development of this approach as a therapeutic intervention for genetic diseases. ..
  14. MOLECULAR ANALYSIS OF FLOWERING GENES OF PHARBITIS NIL
    Anath Das; Fiscal Year: 1991
    ..thaliana genes by similar procedures. Induction of flowering is a critical process in angiosperm lifecycle. Understanding how photoperiod control this process will greatly broaden our knowledge on developmental processes in plants...
  15. CELLULAR REGULATION OF BLOOD CLOTTING
    Marc Shuman; Fiscal Year: 1992
    ..c) to identify and characterize other antigens involved in immune disorders of platelets...
  16. FIBRONECTIN RECEPTOR IN CANDIDA TROPICALIS
    MARGARET HOSTETTER; Fiscal Year: 2003
    ..tropicalis and other pathogenic fungi by Southern blotting or PCR...
  17. MOLECULAR ANALYSIS OF A POTENTIAL TRANSPORT OPERON IN AA
    Karen Novak; Fiscal Year: 2001
    ..Identification of genes involved in the secretion of macromolecules from Aa may lead to the development of strategies aimed at diminishing the virulence potential of this pathogenic microorganism. ..
  18. P30 - CORE GRANT FOR VISION RESEARCH
    Matthew M Lavail; Fiscal Year: 2013
    ..The Core Vision Research Grant has been highly successful in enhancing the productivity of vision research and facilitating collaborative studies on the visual system at UCSF. ..
  19. TWO TYPES OF MONOAMINE OXIDASE
    Jean Shih; Fiscal Year: 2007
    ..unreadable] [unreadable] These studies are important for both basic neuropharmacology and clinical[unreadable] research. ..
  20. CELLULAR CONSEQUENCES OF DEFECTIVE IIB AND IIIA GENES
    Paul Bray; Fiscal Year: 1993
    ..DNA will be analyzed by Southern blotting, and the abnormal alleles will be isolated from genomic DNA libraries...
  21. MOLECULAR PHYSIOLOGY OF HYPERKALEMIC PERIODIC PARALYSIS
    Lawrence Hayward; Fiscal Year: 2001
    ..In eight transgenic mouse lines, wild type or mutant Na channel gene propagation will be verified by Southern blotting, and specificity of expression will be quantitated by an RNAse protection assay...
  22. Amplification Strategies for Detection of Fragile X Trinucleotide Repeats
    ANDREW HADD; Fiscal Year: 2009
    ..This will enable widespread screening to identify carriers and permit earlier diagnosis and intervention for Fragile X Syndrome patients. ..
  23. Signaling in Tissue Injury and Repair
    Robert Diegelmann; Fiscal Year: 2009
    ..The Program Director has made concerted efforts and will continue to strive to attract underrepresented minorities to apply to this unique training program. ..
  24. A New Tuberculin for the Diagnosis of Tuberculosis
    Leo Einck; Fiscal Year: 2001
    ..Diagnosis of TB worldwide is by sputum smear, with 50% accuracy. This new diagnostic is desperately needed. Short term markets will be in developing countries where the test will replace the current skin test as an adjunct diagnostic. ..
  25. MOLECULAR BIOLOGY OF B-CELL CHRONIC LYMPHOCYTIC LEUKEMIA
    Elizabeth Newcomb; Fiscal Year: 1993
    ..This study will identify the transforming activity associated with B-CLL disease. Understanding the genetic mechanism of oncogene activation in B- CLL disease may have potential for improved patient diagnosis and treatment...
  26. GENETIC STUDIES OF HUMAN CENTROMERES
    ETHYLIN JABS; Fiscal Year: 1990
    ..5. Centromeric DNA probes will be used as cytological markers in in situ hybridization studies to determine the arrangement of chromosomes in the interphase nucleus...
  27. INTERACTION BETWEEN BLASTOCYST AND UTERINE ENDOMETRIUM
    R Roberts; Fiscal Year: 1999
    ..genes for the embryonic IFN'S, ovine trophoblast protein-1 (oTP-1) and the related bovine protein, bTP-1, by Southern blotting procedures with CDNA probes that will distinguish these IFN's from related IFN-alphas; ii) To map and ..
  28. BIOMARKERS OF TREATMENT RELATED LEUKEMIA
    CAROLYN FELIX; Fiscal Year: 2004
    ..Risk factors for treatment-related leukemia are poorly understood. Predictive biomarker assays will enable rational modifications of primary cancer therapies and provide new opportunities for early intervention. ..
  29. TENASCIN-X AND THE EHLERS-DANLOS PHENOTYPE
    James Bristow; Fiscal Year: 2002
    ..fibroblasts will be evaluated, and the mutations present on each TN-X mutant allele will be identified by Southern blotting, PCR and the protein truncation test...
  30. CLONAL DOMINANCE OF T-LYMPHOCYTES IN ARTHRITIS
    JAMES KURNICK; Fiscal Year: 1991
    ..These studies are central to questions of in vivo selection of the immune repertoire in inflammatory processes, including auto-immune diseases where tolerance-breaking T lymphocyte clones could be pathogenic...
  31. MOLECULAR IMMUNOGENETICS OF NOVEL MHC TUMOR ANTIGENS
    ROBERT GOODENOW; Fiscal Year: 1991
    ....
  32. PHYSICAL MONITORING OF MEIOTIC DNA RECOMBINATION
    James Haber; Fiscal Year: 1993
    ..Finally, we will pursue our observation that ectopic recombination between sites on two different chromosomes is sometimes accompanied by chromosomal non- disjunction. ..
  33. Anthrax spore-surface antigens for biosensor development
    Manohar John; Fiscal Year: 2004
    ..anthracis as determined by BLAST and Southern blotting experiments...
  34. CHARACTERIZATION OF A PUTATIVE MOUSE EMBRYONIC GPDH GENE
    ROBERT IRELAND; Fiscal Year: 1991
    ....
  35. PHYSICAL MAP FOR HUMAN GENOMIC REGION
    Robert Gemmill; Fiscal Year: 1990
    ..This map should provide a foundation upon which a comprehensive map of the entirety of chromosome 3 can be built...
  36. ONCOGENES & GROWTH FACTORS IN HUMAN GYNECOLOGIC CANCERS
    Lundy Braun; Fiscal Year: 1991
    ..These studies should give us a better understanding of the cellular response to HPV infection and help elucidate the role of human papilloma-viruses in the pathogenesis of genital tract malignancies...
  37. IMMUNE CELL COOPERATION IN HUMAN HAPLOIDENTICAL CHIMERA
    JOSEPH ROBERTS; Fiscal Year: 1990
    ....
  38. PAPILLARY SEROUS CARCINOMA OF THE PERITONEUM
    Samuel Mok; Fiscal Year: 2000
    ..A better understanding of PSCP is of clinical important in the clinical management of patients with familial ovarian cancer syndromes who are considering or have undergone prophylactic oophorectomy. ..
  39. CHROMOSOMAL TRANSLOCATIONS IN CHILDHOOD ACUTE LEUKEMIA
    Nora Heisterkamp; Fiscal Year: 1990
    ..In addition, the proposed research might shed light on the molecular significance of the bcr part of the bcr/ ABL protein, which represents the hallmark of CML...
  40. HUMAN COLOR VISION: PSYCHOPHYSICS & MOLECULAR
    THOMAS PIANTANIDA; Fiscal Year: 1990
    ..color-defective males will be digested with restriction enzymes and the restriction fragments analyzed by Southern blotting. A determination of genotype will be made by stoichiometric analysis of the Southern blots, and restriction ..
  41. SOMATIC MUTATIONS IN CHILDREN
    BARRY FINETTE; Fiscal Year: 2002
    ..analysis of hprt and p53 mutations will be performed using several methods: multiplex PCR, RT-PCR, IPCR, Southern blotting and DNA sequencing...
  42. FRAMEWORK LINKAGE MAPS OF HUMAN CHROMOSOMES 13Q AND 15Q
    Anne Bowcock; Fiscal Year: 1993
    ..reaction (PCR) b) Variable Alu polymorphisms (AluVpa) detected with PCR c) VNTR polymorphisms detected by Southern blotting d) polymorphisms in the 3' regions of genes known to reside on chromosomes 13 and 15...
  43. NON-RADIOACTIVE SOUTHERN BLOTTING DETECTION SYSTEM
    Richard Powell; Fiscal Year: 2000
    Fast, convenient detection and visualization reagents will be developed for Southern blotting, based upon the 1.4 nm Nanogold gold cluster label combined with autometallographic enhancement...
  44. INTRAVIRION REVERSE TRANSCRIPTION AND HIV-1 PATHOGENESIS
    Roger Pomerantz; Fiscal Year: 1999
    ..direct radiolabelling of de novo synthesis of intravirion DNA, plus modified RNase protection assays and Southern blotting using strand specific probes, and certain modifying enzymes...
  45. HL-A SYSTEM AND THE GENETICS OF JUVENILE DIABETES
    Pablo Rubinstein; Fiscal Year: 1990
    ..b>Southern blotting of genomic DNA restriction fragments (RF) with cDNA probes corresponding to the alpha and beta chain genes ..
  46. MOLECULAR STUDIES OF CYSTIC FIBROSIS
    Arthur Beaudet; Fiscal Year: 1990
    ..Conserved and transcribed regions will be identified using Southern blotting with DNA from various species as well as Northern blotting analysis...
  47. NOVEL MITOGENIC REGULATORY GENE
    Irwin Gelman; Fiscal Year: 1999
    ..This down-regulation is not the result of gross loss or damage to the 322 gene allele, as determined by southern blotting. Activation of a ts-src allele or addition of fetal calf serum to starved cells results in a rapid increase ..
  48. PATHOGENESIS OF HUMAN T-CELL LEUKEMIAS/LYMPHOMAS
    Stuart Lessin; Fiscal Year: 1990
    ..This study should result in a better understanding of the genetic mechanisms leading to T-cell neoplasia and should provide probes to be used in the diagnosis of this human malignancy...
  49. MOLECULAR GENETICS OF PAR-3
    Lisa Cupit; Fiscal Year: 2004
    ..The candidate's long-term goal is the pursuit of a career in academic medicine. ..
  50. G PROTEIN SIGNALING AND SKIN BIOLOGY
    Barbara McAlpine; Fiscal Year: 2000
    ..An efficient strategy for cloning, screening by southern blotting and sequencing unique cDNAs has been established...
  51. Single molecule fluorescence detection of fragile X mutations
    Liangjing Chen; Fiscal Year: 2009
    ..Our long-term goal is to develop Fragile X mutation screening assays that will identify individuals at-risk for developing or passing on these genes. ..
  52. Impact of Genetic Variation on Th Cell Differentiation
    Donata Vercelli; Fiscal Year: 2005
    ....
  53. IMMUNOBIOLOGY OF NORMAL AND LEUKEMIC B-CELL PRECURSORS
    Fatih Uckun; Fiscal Year: 1992
    ....
  54. Enzymatic Metallography for Ultrasensitive Biodetection
    Richard Powell; Fiscal Year: 2006
    ..For DNA and RNA in situ hybridization and Southern Blotting, enhancements will be pursued to maximize sensitivity: the enzymatic metallation will be accelerated by ..
  55. Cellular Response to Retroviral DNA Integration
    Rene Daniel; Fiscal Year: 2007
    ..They should also significantly enhance our understanding of the function of these important cellular pathways. [unreadable] [unreadable]..
  56. NOVEL FUSION PROTEIN IN CMML
    Theodora Ross; Fiscal Year: 2002
    ..b>Southern blotting analysis has identified a breakpoint in this patient at the same genomic localization in the PDGFBR as the t(..
  57. CELLULAR PHENOTYPES OF SALT SENSITIVITY
    Abraham Aviv; Fiscal Year: 2001
    ..The investigators state that the results of the study will generate a novel perspective about phenotypes of salt sensitivity and, an insight into the cardiovascular effects of high salt in humans. ..
  58. Human Telomere Genetics
    Abraham Aviv; Fiscal Year: 2008
    ..Results will serve to define genes as determinants of telomere length. Such information would lead to new diagnostic and therapeutic approaches to treat age-related diseases. ..
  59. Genetics of the Sodium-Lithium Countertransport
    Abraham Aviv; Fiscal Year: 2008
    ..abstract_text> ..
  60. Telomeres and Vascular Aging
    Abraham Aviv; Fiscal Year: 2007
    ..abstract_text> ..
  61. Hematopoietic Potential of Muscle-Derived Cells
    Hans Peter Kiem; Fiscal Year: 2004
    ..abstract_text> ..
  62. INSULIN RECEPTOR RELATED AND THE KIDNEY
    Carlton Bates; Fiscal Year: 2002
    ..After being subjected to systemic alkalosis, IRR-null mouse kidneys will be examined to determine if the intercalated cells have appropriately altered their morphology and intracellular distribution of proton transporters. ..