protein array analysis

Summary

Summary: Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.

Top Publications

  1. pmc Diversity and complexity in DNA recognition by transcription factors
    Gwenael Badis
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5S 3E1, Canada
    Science 324:1720-3. 2009
  2. pmc Curated collection of yeast transcription factor DNA binding specificity data reveals novel structural and gene regulatory insights
    Raluca Gordân
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Genome Biol 12:R125. 2011
  3. ncbi Autoantibody signatures in prostate cancer
    Xiaoju Wang
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    N Engl J Med 353:1224-35. 2005
  4. pmc A prospective analysis of the Ab response to Plasmodium falciparum before and after a malaria season by protein microarray
    Peter D Crompton
    Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20852, USA
    Proc Natl Acad Sci U S A 107:6958-63. 2010
  5. pmc Universal protein-binding microarrays for the comprehensive characterization of the DNA-binding specificities of transcription factors
    Michael F Berger
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Protoc 4:393-411. 2009
  6. ncbi Reverse phase protein array: validation of a novel proteomic technology and utility for analysis of primary leukemia specimens and hematopoietic stem cells
    Raoul Tibes
    Department of Medical Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030 4095, USA
    Mol Cancer Ther 5:2512-21. 2006
  7. pmc Diagnosis of Parkinson's disease based on disease-specific autoantibody profiles in human sera
    Min Han
    University of Medicine and Dentistry of New Jersey Graduate School of Biomedical Sciences at the School of Osteopathic Medicine, Stratford, New Jersey, United States of America
    PLoS ONE 7:e32383. 2012
  8. pmc Profiling the humoral immune response to infection by using proteome microarrays: high-throughput vaccine and diagnostic antigen discovery
    D Huw Davies
    Center for Virus Research, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 102:547-52. 2005
  9. pmc Protein acetylation microarray reveals that NuA4 controls key metabolic target regulating gluconeogenesis
    Yu yi Lin
    High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 136:1073-84. 2009
  10. pmc Profiling humoral immune responses to P. falciparum infection with protein microarrays
    Denise L Doolan
    Naval Medical Research Center, Silver Spring, MD, USA
    Proteomics 8:4680-94. 2008

Detail Information

Publications325 found, 100 shown here

  1. pmc Diversity and complexity in DNA recognition by transcription factors
    Gwenael Badis
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5S 3E1, Canada
    Science 324:1720-3. 2009
    ..This complexity in DNA recognition may be important in gene regulation and in the evolution of transcriptional regulatory networks...
  2. pmc Curated collection of yeast transcription factor DNA binding specificity data reveals novel structural and gene regulatory insights
    Raluca Gordân
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Genome Biol 12:R125. 2011
    ..Recent surveys have examined the DNA binding specificities of most Saccharomyces cerevisiae TFs, but a comprehensive evaluation of their data has been lacking...
  3. ncbi Autoantibody signatures in prostate cancer
    Xiaoju Wang
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    N Engl J Med 353:1224-35. 2005
    ..New biomarkers, such as autoantibody signatures, may improve the early detection of prostate cancer...
  4. pmc A prospective analysis of the Ab response to Plasmodium falciparum before and after a malaria season by protein microarray
    Peter D Crompton
    Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20852, USA
    Proc Natl Acad Sci U S A 107:6958-63. 2010
    ....
  5. pmc Universal protein-binding microarrays for the comprehensive characterization of the DNA-binding specificities of transcription factors
    Michael F Berger
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Protoc 4:393-411. 2009
    ..Multiple proteins can be tested in parallel in separate chambers on a single microarray, enabling the processing of a dozen or more TFs in a single day...
  6. ncbi Reverse phase protein array: validation of a novel proteomic technology and utility for analysis of primary leukemia specimens and hematopoietic stem cells
    Raoul Tibes
    Department of Medical Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030 4095, USA
    Mol Cancer Ther 5:2512-21. 2006
    ....
  7. pmc Diagnosis of Parkinson's disease based on disease-specific autoantibody profiles in human sera
    Min Han
    University of Medicine and Dentistry of New Jersey Graduate School of Biomedical Sciences at the School of Osteopathic Medicine, Stratford, New Jersey, United States of America
    PLoS ONE 7:e32383. 2012
    ..0%, 96.6%, and 100%, respectively. Results demonstrate that serum autoantibodies can be used as highly specific and accurate biomarkers for PD diagnosis throughout the course of the disease...
  8. pmc Profiling the humoral immune response to infection by using proteome microarrays: high-throughput vaccine and diagnostic antigen discovery
    D Huw Davies
    Center for Virus Research, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 102:547-52. 2005
    ..These results verify this platform as a rapid way to comprehensively scan humoral immunity from vaccinated or infected humans and animals...
  9. pmc Protein acetylation microarray reveals that NuA4 controls key metabolic target regulating gluconeogenesis
    Yu yi Lin
    High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 136:1073-84. 2009
    ..Our findings demonstrate a regulatory function for the NuA4 complex in glucose metabolism and life span by acetylating a critical metabolic enzyme...
  10. pmc Profiling humoral immune responses to P. falciparum infection with protein microarrays
    Denise L Doolan
    Naval Medical Research Center, Silver Spring, MD, USA
    Proteomics 8:4680-94. 2008
    ....
  11. pmc A protein microarray-based analysis of S-nitrosylation
    Matthew W Foster
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 106:18948-53. 2009
    ....
  12. ncbi Profiling of humoral immune responses to influenza viruses by using protein microarray
    M Koopmans
    Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
    Clin Microbiol Infect 18:797-807. 2012
    ..Clinical and vaccination studies, but also exposure studies, should take these findings into consideration, as they may indicate some level of humoral immunity not measured by HI assays...
  13. ncbi Antibody-based proteomics: analysis of signaling networks using reverse protein arrays
    Hans Voshol
    Novartis Institutes for BioMedical Research, Basel, Switzerland
    FEBS J 276:6871-9. 2009
    ....
  14. pmc Identification of autoantibody clusters that best predict lupus disease activity using glomerular proteome arrays
    Quan Zhen Li
    Department of Internal Medicine Rheumatology, Center for Immunology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 8884, USA
    J Clin Invest 115:3428-39. 2005
    ..Hence, the glomerular proteome array promises to be a powerful analytical tool for uncovering novel autoantibody disease associations and for distinguishing patients at high risk for end-organ disease...
  15. pmc Diagnosis of Alzheimer's disease based on disease-specific autoantibody profiles in human sera
    Eric Nagele
    Durin Technologies, Inc, New Brunswick, New Jersey, United States of America
    PLoS ONE 6:e23112. 2011
    ..Results demonstrate that serum autoantibodies can be used effectively as highly-specific and accurate biomarkers to diagnose AD throughout the course of the disease...
  16. ncbi Robust-linear-model normalization to reduce technical variability in functional protein microarrays
    Andrea Sboner
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    J Proteome Res 8:5451-64. 2009
    ..Hence, we show RLM normalization is better suited to protein arrays than approaches used for DNA microarrays...
  17. ncbi Antigen microarrays: descriptive chemistry or functional immunomics?
    Jozsef Prechl
    Research Group of Immunology of the Hungarian Academy of Sciences, Eotvos Lorand University, Budapest 1117, Hungary
    Trends Immunol 31:133-7. 2010
    ..Furthermore, we could generate functional immune profiles characterizing general immune responsiveness of the individual by designing arrays incorporating epitope collections from diverse subsets of antibody targets...
  18. ncbi A roadmap to generate renewable protein binders to the human proteome
    Karen Colwill
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Nat Methods 8:551-8. 2011
    ..We believe that this work serves as a foundation and template for future larger-scale studies to create renewable protein binders...
  19. ncbi A general method for characterization of humoral immunity induced by a vaccine or infection
    Joseph Barten Legutki
    Center for Innovations in Medicine, The Biodesign Institute, School of Life Sciences, Arizona State University, Tempe, AZ 85287 5901, USA
    Vaccine 28:4529-37. 2010
    ..Taken together the data suggest that immunosignaturing on a random peptide array can serve as a universal platform to assess antibody status in ways that cannot be replicated by conventional immunological assays...
  20. pmc Next-generation high-density self-assembling functional protein arrays
    Niroshan Ramachandran
    Harvard Institute of Proteomics, Harvard Medical School, 320 Charles Street, Cambridge, Massachusetts 02141, USA
    Nat Methods 5:535-8. 2008
    ..This method will enable various experimental approaches to study protein function in high throughput...
  21. ncbi High throughput identification of potential Arabidopsis mitogen-activated protein kinases substrates
    Tanja Feilner
    Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics MPI MG, Ihnestr 73, 14195 Berlin, Germany
    Mol Cell Proteomics 4:1558-68. 2005
    ..Follow-up in vivo experiments are essential to evaluate their physiological relevance...
  22. ncbi Optimizing antibody immobilization strategies for the construction of protein microarrays
    Paul Peluso
    Zyomyx, Inc, 26101 Research Road, Hayward, CA 94544, USA
    Anal Biochem 312:113-24. 2003
    ..These results emphasize the importance of attaching proteins to surfaces such that their binding sites are oriented toward the solution phase...
  23. ncbi Antibody-based proteomics for human tissue profiling
    Mathias Uhlen
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 10691 Stockholm, Sweden
    Mol Cell Proteomics 4:384-93. 2005
    ....
  24. ncbi Quantitative protein analysis from formalin-fixed tissues: implications for translational clinical research and nanoscale molecular diagnosis
    K F Becker
    Technische Universitat, Institut fur Pathologie, Munchen, Germany
    J Pathol 211:370-8. 2007
    ..This technique may also be used to identify, characterize, and validate known and new protein markers in a variety of human diseases...
  25. pmc PDZ domain binding selectivity is optimized across the mouse proteome
    Michael A Stiffler
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Science 317:364-9. 2007
    ..We predict that focusing on families of interaction domains, which facilitates the integration of experimentation and modeling, will play an increasingly important role in future investigations of protein function...
  26. pmc Protein microarray signature of autoantibody biomarkers for the early detection of breast cancer
    Karen S Anderson
    Cancer Vaccine Center, Dana Farber Cancer Institute, Boston, Massachusetts, USA
    J Proteome Res 10:85-96. 2011
    ..05). Using all 28 AAb, a classifier was identified with a sensitivity of 80.8% and a specificity of 61.6% (AUC = 0.756). These are potential biomarkers for the early detection of breast cancer...
  27. pmc Systems biology approach predicts antibody signature associated with Brucella melitensis infection in humans
    Li Liang
    Department of Medicine, Division of Infectious Diseases, University of California, Irvine, California 92697, United States
    J Proteome Res 10:4813-24. 2011
    ..This systems biology approach provides an empirical basis for understanding the breadth and specificity of the immune response to B. melitensis and a new framework for comparing the humoral responses against other microorganisms...
  28. ncbi Comprehensive identification of human bZIP interactions with coiled-coil arrays
    John R S Newman
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Science 300:2097-101. 2003
    ..Biophysical studies in solution support the relative binding strengths observed with the arrays. New associations provide insights into the circadian clock and the unfolded protein response...
  29. pmc Human body fluid proteome analysis
    Shen Hu
    School of Dentistry, Division of Oral Biology and Medicine, Dental Research Institute, University of California, Los Angeles, CA 90095, USA
    Proteomics 6:6326-53. 2006
    ..With the advances made in proteomics technologies, the impact of HBFP analysis in the search for clinically relevant disease biomarkers would be realized in the future...
  30. ncbi Proteome-wide analysis of the serological response to vaccinia and smallpox
    D Huw Davies
    Center for Virus Research, University of California, Irvine, CA 92697, USA
    Proteomics 7:1678-86. 2007
    ....
  31. ncbi Protein microarrays: meeting analytical challenges for clinical applications
    Lance A Liotta
    FDA NCI Clinical Proteomics Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Cancer Cell 3:317-25. 2003
    ....
  32. pmc Glycome diagnosis of human induced pluripotent stem cells using lectin microarray
    Hiroaki Tateno
    From the Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    J Biol Chem 286:20345-53. 2011
    ..Hence, the high density lectin microarray has proved to be valid for not only comprehensive analysis of glycans but also diagnosis of stem cells under the concept of the cellular glycome...
  33. pmc Discovery of potential diagnostic and vaccine antigens in herpes simplex virus 1 and 2 by proteome-wide antibody profiling
    Mina Kalantari-Dehaghi
    Division of Infectious Diseases, School of Medicine, University of California, Irvine, Irvine, California, USA
    J Virol 86:4328-39. 2012
    ..e., recognized by both HSV-1- and HSV-2-positive donors). Both membrane and nonmembrane virion proteins were antigenic, although type-specific antigens were enriched for membrane proteins, despite being expressed in E. coli...
  34. pmc Nε-lysine acetylation of a bacterial transcription factor inhibits Its DNA-binding activity
    Sandy Thao
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, United States of America
    PLoS ONE 5:e15123. 2010
    ..These findings further the likelihood that reversible N(ε)-Lys acetylation of transcription factors is a mode of regulation of gene expression used by all cells...
  35. ncbi A novel strategy for mammalian cell surface glycome profiling using lectin microarray
    Hiroaki Tateno
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2, 1 1 1 Umezono, Ibaraki 305 8568, Japan
    Glycobiology 17:1138-46. 2007
    ..These results demonstrate that the technique provides a novel strategy for profiling global changes of the mammalian cell surface glycome...
  36. ncbi Identification of human embryonic stem cell surface markers by combined membrane-polysome translation state array analysis and immunotranscriptional profiling
    Gabriel Kolle
    Institute for Molecular Bioscience, The University of Queensland, Queensland, Australia
    Stem Cells 27:2446-56. 2009
    ....
  37. pmc Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments
    Yoav Soen
    Department of Biochemistry, Stanford University, Stanford, CA 94305 5307, USA
    Mol Syst Biol 2:37. 2006
    ....
  38. pmc Monitoring proteins and protein networks using reverse phase protein arrays
    Bridget Wilson
    Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA
    Dis Markers 28:225-32. 2010
    ....
  39. ncbi Transcript and protein expression profiles of the NCI-60 cancer cell panel: an integromic microarray study
    Uma T Shankavaram
    Genomics and Bioinformatics Group, Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute NIH, Bethesda, MD 20892, USA
    Mol Cancer Ther 6:820-32. 2007
    ..71). Because the transcript-based technologies are more mature and are currently able to assess larger numbers of genes at one time, they continue to be useful, even when the ultimate aim is information about proteins...
  40. ncbi Identification of a novel autoantigen in inflammatory bowel disease by protein microarray
    Nathalie Vermeulen
    Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium
    Inflamm Bowel Dis 17:1291-300. 2011
    ..Patients with inflammatory bowel disease (IBD) display immunoreactivity to self-antigens and microbial antigens. We used a protein microarray approach to identify novel autoantigens in IBD...
  41. pmc Peptide microarray analysis of in silico-predicted epitopes for serological diagnosis of Toxoplasma gondii infection in humans
    Pavlo Maksimov
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Clin Vaccine Immunol 19:865-74. 2012
    ..gondii epitopes as candidate antigens for serological diagnosis...
  42. pmc Immune complex signatures of patients with active and inactive SLE revealed by multiplex protein binding analysis on antigen microarrays
    Krisztián Papp
    Department of Immunology, Eotvos Lorand University, Budapest, Hungary
    PLoS ONE 7:e44824. 2012
    ..Our observations confirmed previously known markers of disease and showed that C3 and C4 deposition data were at least as powerful as Ig binding data in separating the study groups...
  43. pmc Leptospiral outer membrane protein microarray, a novel approach to identification of host ligand-binding proteins
    Marija Pinne
    Research Service, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California, USA
    J Bacteriol 194:6074-87. 2012
    ..Protein microarrays are valuable tools for high-throughput identification of novel host ligand-binding proteins that have the potential to play key roles in the virulence mechanisms of pathogens...
  44. pmc Assessment of algorithms for inferring positional weight matrix motifs of transcription factor binding sites using protein binding microarray data
    Yaron Orenstein
    Blavatnik School of Computer Science, Tel Aviv University, Israel
    PLoS ONE 7:e46145. 2012
    ..Interestingly, in predicting high-affinity binding, all methods give far poorer results for in vivo assays compared to in vitro assays...
  45. ncbi Computational analysis of high-density peptide microarray data with application from systemic sclerosis to multiple sclerosis
    Michael Hecker
    Department of Immunology, University of Rostock, Rostock, Germany
    Autoimmun Rev 11:180-90. 2012
    ..Presented experimental and bioinformatic approach can be applied in the same manner for multiple sclerosis research...
  46. ncbi Molecular analysis of HER2 signaling in human breast cancer by functional protein pathway activation mapping
    Julia D Wulfkuhle
    Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, Virginia 20110, USA
    Clin Cancer Res 18:6426-35. 2012
    ....
  47. ncbi The natural autoantibody repertoire in newborns and adults: a current overview
    Asaf Madi
    Faculty of Medicine, Tel Aviv University, Israel
    Adv Exp Med Biol 750:198-212. 2012
    ..Thus, repertoires of autoantibodies in healthy subjects, the immunological homunculus, are structured in hierarchies of antigen reactivities...
  48. pmc High-Throughput GoMiner, an 'industrial-strength' integrative gene ontology tool for interpretation of multiple-microarray experiments, with application to studies of Common Variable Immune Deficiency (CVID)
    Barry R Zeeberg
    Genomics and Bioinformatics Group, Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    BMC Bioinformatics 6:168. 2005
    ..We wanted to provide a computational resource that automates the analysis of multiple microarrays and then integrates the results across all of them in useful exportable output files and visualizations...
  49. ncbi Protein microarray-based comparison of HER2, estrogen receptor, and progesterone receptor status in core biopsies and surgical specimens from FFPE breast cancer tissues
    Daniela Berg
    Department of Pathology, Technische Universitat Munchen, Munich, Germany
    Appl Immunohistochem Mol Morphol 19:300-5. 2011
    ..These results might facilitate the implementation of RPPA technology in routine clinical settings...
  50. pmc Functional proteomic analysis of advanced serous ovarian cancer using reverse phase protein array: TGF-beta pathway signaling indicates response to primary chemotherapy
    Mark S Carey
    Department of Systems Biology, University of Texas M D Anderson Cancer Center, Houston, Texas, USA
    Clin Cancer Res 16:2852-60. 2010
    ..Using reverse phase protein array, we measured protein expression associated with response to primary chemotherapy in patients with advanced-stage, high-grade serous ovarian cancer...
  51. pmc Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes
    Li A Kung
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06620 8103, USA
    Mol Syst Biol 5:308. 2009
    ....
  52. pmc Gene expression profiles of primary colorectal carcinomas, liver metastases, and carcinomatoses
    Kristine Kleivi
    Department of Genetics, Institute for Cancer Research, Rikshospitalet Radiumhospitalet Medical Center, Oslo, Norway
    Mol Cancer 6:2. 2007
    ..Therefore, the aim of the present study is to investigate and compare the gene expression patterns of primary carcinomas (n = 18), liver metastases (n = 4), and carcinomatoses (n = 4), relative to normal samples from the large bowel...
  53. ncbi Producing reverse phase protein microarrays from formalin-fixed tissues
    Claudia Wolff
    Institut fur Pathologie, Technische Universitat Munchen, Munich, Germany
    Methods Mol Biol 785:123-40. 2011
    ..In this chapter, we describe a protein extraction protocol established in our laboratory combined with the use of reverse phase protein microarray...
  54. pmc Autoantibody approach for serum-based detection of head and neck cancer
    Ho Sheng Lin
    Department of Surgery, John D Dingell VA Medical Center, Wayne State University, Detroit, Michigan 48201, USA
    Cancer Epidemiol Biomarkers Prev 16:2396-405. 2007
    ....
  55. pmc Glycan microarray profiling of parasite infection sera identifies the LDNF glycan as a potential antigen for serodiagnosis of trichinellosis
    Carmen Aranzamendi
    Centre for Infectious Disease Control Netherlands, National Institute of Public Health and Environment, Bilthoven, The Netherlands
    Exp Parasitol 129:221-6. 2011
    ....
  56. ncbi High-density peptide arrays
    Frank Breitling
    German Cancer Research Center, Im Neuenheimer Feld 580, Heidelberg, Germany
    Mol Biosyst 5:224-34. 2009
    ..However, peptide arrays lag behind oligonucleotide arrays. This review discusses recent developments in the field with an emphasis on methods that lead to very high-density peptide arrays...
  57. pmc Improved models for transcription factor binding site identification using nonindependent interactions
    Yue Zhao
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63108, USA
    Genetics 191:781-90. 2012
    ..Having more accurate models of specificity greatly improves the interpretation of in vivo TF localization data, such as from chromatin immunoprecipitation followed by sequencing (ChIP-seq) experiments...
  58. pmc Glycosylation variants of mucins and CEACAMs as candidate biomarkers for the diagnosis of pancreatic cystic neoplasms
    Brian B Haab
    Van Andel Research Institute, Grand Rapids, MI, USA
    Ann Surg 251:937-45. 2010
    ..Here we explored whether glycosylation variants on specific proteins in cyst fluid samples could serve as biomarkers to aid in this diagnosis...
  59. pmc Histone H3K4me3 binding is required for the DNA repair and apoptotic activities of ING1 tumor suppressor
    P V Peña
    Department of Pharmacology, University of Colorado Health Sciences Center, 12801 East 17th Avenue, Aurora, CO 80045 0511, USA
    J Mol Biol 380:303-12. 2008
    ..Together, our findings reveal the critical role of the H3K4me3 interaction in mediating cellular responses to genotoxic stresses and offer new insight into the molecular mechanism underlying the tumor suppressive activity of ING1...
  60. ncbi Antibody arrays in cancer research
    Brian B Haab
    Van Andel Research Institute, Grand Rapids, Michigan 49503, USA
    Mol Cell Proteomics 4:377-83. 2005
    ..This article reviews the various types of antibody array methods and their applications to cancer research...
  61. pmc Protein array identification of substrates of the Epstein-Barr virus protein kinase BGLF4
    Jian Zhu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, BRB 333, 733 N Broadway Ave, Baltimore, MD 21205, USA
    J Virol 83:5219-31. 2009
    ..We propose that BGLF4 contributes to effective EBV lytic cycle progression, not only through phosphorylation of EBV lytic DNA replication and virion proteins, but also by interfering with the EBNA1 replication function...
  62. ncbi Circulating interleukin (IL)-8, IL-2R, IL-12, and IL-15 levels are independently prognostic in primary myelofibrosis: a comprehensive cytokine profiling study
    Ayalew Tefferi
    Mayo Clinic, 200 First St SW, Rochester, MN 55905, USA
    J Clin Oncol 29:1356-63. 2011
    ..This study describes the spectrum of plasma cytokine abnormalities in primary myelofibrosis (PMF) and examines their phenotypic correlates and prognostic significance...
  63. ncbi Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion
    Kai Hilpert
    Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall Research Station, Vancouver, British Columbia, Canada V6T 1Z3
    Nat Protoc 2:1333-49. 2007
    ..The time required to complete the protocol depends on the number and length of the peptides. For example, 400 9-mer peptides can be synthesized within 6 days...
  64. pmc Diverse protein kinase interactions identified by protein microarrays reveal novel connections between cellular processes
    Joseph Fasolo
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
    Genes Dev 25:767-78. 2011
    ..Our results further demonstrate that protein microarrays uncover a diverse set of interactions not observed previously...
  65. ncbi Serum protein profiling in mice: identification of Factor XIIIa as a potential biomarker for muscular dystrophy
    Sharmini Alagaratnam
    Center for Human and Clinical Genetics, Leiden University Medical Center, Leiden, The Netherlands
    Proteomics 8:1552-63. 2008
    ..This work underlines the translational aspect of serum protein profiling in mice and supports a further study with serum from patients with neuromuscular disorders...
  66. pmc Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray
    Claudia List
    Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland
    PLoS Negl Trop Dis 4:e771. 2010
    ....
  67. ncbi Reverse-phase protein lysate microarrays for cell signaling analysis
    Brett Spurrier
    Molecular Translational Technology Section, Molecular Therapeutics Program, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
    Nat Protoc 3:1796-808. 2008
    ....
  68. ncbi Prognostic impact of alterations in P-cadherin expression and related cell adhesion markers in endometrial cancer
    Ingunn M Stefansson
    Department of Pathology, The Gade Institute, Haukeland University Hospital, Bergen, Norway
    J Clin Oncol 22:1242-52. 2004
    ..We wanted to examine the expression pattern and prognostic value of these molecules in a population-based series of endometrial carcinoma patients...
  69. ncbi Protein microarrays for diagnostic assays
    Michael Hartmann
    School of Chemical Science and Engineering, Department of Analytical Chemistry, Royal Institute of Technology, 10044, Stockholm, Sweden
    Anal Bioanal Chem 393:1407-16. 2009
    ..This article reviews the current state of protein microarrays and discusses developments and future demands relating to protein arrays in their role as multiplexed immunoassays in the field of diagnostics...
  70. ncbi Serum proteome profiling of metastatic breast cancer using recombinant antibody microarrays
    Anders Carlsson
    Department of Immunotechnology, Lund University, BMC D13, SE 221 84 Lund, Sweden
    Eur J Cancer 44:472-80. 2008
    ..In a longer perspective, antibody microarray analysis could provide a tool for the development of improved diagnostics and intensified biomarker discovery for breast cancer patients...
  71. ncbi Design of recombinant antibody microarrays for complex proteome analysis: choice of sample labeling-tag and solid support
    Christer Wingren
    Department of Immunotechnology, BMC D13, Lund University, Lund, Sweden
    Proteomics 7:3055-65. 2007
    ....
  72. ncbi Three biomarkers identified from serum proteomic analysis for the detection of early stage ovarian cancer
    Zhen Zhang
    Department of Pathology, Biomarker Discovery Center, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 64:5882-90. 2004
    ..These biomarkers demonstrated the potential to improve the detection of early stage ovarian cancer...
  73. pmc A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes
    Tanja Scherrer
    Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland
    PLoS ONE 5:e15499. 2010
    ..Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation...
  74. ncbi Non-parametric quantification of protein lysate arrays
    Jianhua Hu
    Department of Bioinformatics and Computational Biology, University of Texas M D Anderson Cancer Center, TX, USA
    Bioinformatics 23:1986-94. 2007
    ..Existing approaches to quantify protein arrays use parametric response curves fit to dilution series data. The results can be biased when the parametric function does not fit the data...
  75. pmc Bladder cancer associated glycoprotein signatures revealed by urinary proteomic profiling
    Paweena Kreunin
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA
    J Proteome Res 6:2631-9. 2007
    ....
  76. ncbi Molecular profiling of the immune response in colon cancer using protein microarrays: occurrence of autoantibodies to ubiquitin C-terminal hydrolase L3
    Myeong J Nam
    Department of Pediatrics, University of Michigan Medical School, 1150 W Medical Drive, Ann Arbor, MI 48109 0656, USA
    Proteomics 3:2108-15. 2003
    ..Our findings indicate the occurrence of an immune response to a broad set of antigens in colon cancer and the feasibility of identifying the antigenic targets using a combination of protein microarrays and mass spectrometry...
  77. ncbi Functional peptide microarrays for specific and sensitive antibody diagnostics
    Heiko Andresen
    Fraunhofer Institute for Biomedical Engineering, Department of Molecular Bioanalytics and Bioelectronics, Potsdam Nuthetal, Germany
    Proteomics 6:1376-84. 2006
    ..We further demonstrate that the peptide array is capable of detecting low-picomolar concentrations of mAbs in buffered solutions and diluted human serum with high specificity...
  78. ncbi Protein arrays as tools for serum autoantibody marker discovery in cancer
    Gregor Kijanka
    Centre for Human Proteomics, Royal College of Surgeons in Ireland, Dublin 2, Ireland
    J Proteomics 72:936-44. 2009
    ....
  79. ncbi Proteomic analysis of individual human embryos to identify novel biomarkers of development and viability
    Mandy G Katz-Jaffe
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80113, USA
    Fertil Steril 85:101-7. 2006
    ..To develop a method to analyze the proteome of individual human blastocysts and identify differentially expressed proteins prior to implantation...
  80. ncbi Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening
    Chenggang Wu
    Department of Biochemistry and the Siebens Drake Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada
    Proteomics 7:1775-85. 2007
    ....
  81. ncbi Protein microarrays: molecular profiling technologies for clinical specimens
    Virginia Espina
    FDA NCI Clinical Proteomics Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute NIH, Building 10 Room B1B53, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Proteomics 3:2091-100. 2003
    ..A subclass of protein microarrays, Reverse Phase Arrays, created to meet these challenges, has been optimized for use with tissue specimens, and is now in use for the analysis of biopsy samples for clinical trial research...
  82. pmc Chromatin immunoprecipitation and microarray-based analysis of protein location
    Tong Ihn Lee
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Protoc 1:729-48. 2006
    ..These protocols require approximately 1 week to complete once sufficient numbers of cells have been obtained, and have been used to produce robust, high-quality ChIP-chip results in many different cell and tissue types...
  83. pmc Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications
    Emre Ozkumur
    Department of Electrical and Computer Engineering, Boston University, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 105:7988-92. 2008
    ..The presented method is particularly relevant for protein microarray analysis because it is label-free, simple, sensitive, and easily scales to high-throughput...
  84. ncbi Protein biomarker discovery and validation: the long and uncertain path to clinical utility
    Nader Rifai
    Departments of Laboratory Medicine and Pathology, Children s Hospital Boston and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    Nat Biotechnol 24:971-83. 2006
    ....
  85. ncbi Reliability of tissue microarrays in detecting protein expression and gene amplification in breast cancer
    Daohai Zhang
    Molecular Diagnosis Centre, National University Hospital, National University of Singapore, Singapore
    Mod Pathol 16:79-84. 2003
    ....
  86. pmc New tools for classification and monitoring of autoimmune diseases
    Holden T Maecker
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Nat Rev Rheumatol 8:317-28. 2012
    ..These analyses are revealing a more comprehensive and interconnected view of the immune system than ever before and should have an important role in directing future treatment approaches for autoimmune diseases...
  87. pmc Protein array autoantibody profiles for insights into systemic lupus erythematosus and incomplete lupus syndromes
    Q Z Li
    Center for Immunology, University of Texas Southwestern Medical Center, Dalls, TX, USA
    Clin Exp Immunol 147:60-70. 2007
    ..Determining whether the IgM aurotreactivity that predominates in ILE represents an early stage prior to IgG switching or is persistent and relatively protective will require further longitudinal studies...
  88. ncbi Validation of peptide epitope microarray experiments and extraction of quality data
    Tatjana Nahtman
    Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden
    J Immunol Methods 328:1-13. 2007
    ....
  89. pmc RAP: accurate and fast motif finding based on protein-binding microarray data
    Yaron Orenstein
    Blavatnik School of Computer Science, Tel Aviv University, Tel Aviv, Israel
    J Comput Biol 20:375-82. 2013
    ..A large drop in the results quality of all methods is observed between in vitro and in vivo binding prediction. The software is available on acgt.cs.tau.ac.il/rap...
  90. ncbi Investigation of autoantibody profiles for cerebrospinal fluid biomarker discovery in patients with relapsing-remitting multiple sclerosis
    Natascha Helena Beyer
    Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark
    J Neuroimmunol 242:26-32. 2012
    ..A combination of the 10-15 most significant proteins may be investigated further as autoantigens for diagnostic purposes. Additional investigations may include minimizing the number of proteins used in such diagnostic tests...
  91. pmc Experimental infections of the reservoir species Peromyscus leucopus with diverse strains of Borrelia burgdorferi, a Lyme disease agent
    ELISABETH BAUM
    Departments of Microbiology and Molecular Genetics, Medicine, and Ecology and Evolutionary Biology, University of California, Irvine, CA, USA
    MBio 3:e00434-12. 2012
    ..We propose that for B. burgdorferi, there is selection in reservoir species for (i) sequence diversity, as for OspC and VlsE, and (ii) the presence or absence of polymorphisms, as for BBK07 and BBK12...
  92. pmc Peptide-MHC cellular microarray with innovative data analysis system for simultaneously detecting multiple CD4 T-cell responses
    Xinhui Ge
    Benaroya Research Institute at Virginia Mason, Seattle, Washington, USA
    PLoS ONE 5:e11355. 2010
    ....
  93. pmc Identification of the feline humoral immune response to Bartonella henselae infection by protein microarray
    ADAM VIGIL
    Division of Infectious Diseases, Department of Medicine, University of California Irvine, Irvine, California, United States of America
    PLoS ONE 5:e11447. 2010
    ..Understanding the complex interactions between the host's immune system and bacterial pathogens is central to the field of infectious diseases and to the development of effective diagnostics and vaccines...
  94. pmc An antibody profile of systemic lupus erythematosus detected by antigen microarray
    Ittai Fattal
    Department of Nephrology, Rabin Medical Center, Petach Tikva, Israel
    Immunology 130:337-43. 2010
    ..Thus, SLE is characterized by an enduring antibody profile irrespective of clinical state. The association of SLE with decreased IgM natural autoantibodies suggests that these autoantibodies might enhance resistance to SLE...
  95. ncbi Protein array analysis of oligomerization-induced changes in alpha-synuclein protein-protein interactions points to an interference with Cdc42 effector proteins
    C Schnack
    Boehringer Ingelheim Pharma GmbH and Co KG, CNS Research, Biberach an der Riss, Germany
    Neuroscience 154:1450-7. 2008
    ..Our data indicate that components of small GTPase signal transduction pathways may be directly targeted by alpha-synuclein oligomers which potentially leads to signaling deficits and neurodegeneration...
  96. ncbi Construction of a natural mucin microarray and interrogation for biologically relevant glyco-epitopes
    Michelle Kilcoyne
    Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland
    Anal Chem 84:3330-8. 2012
    ..This study introduces natural mucin microarrays as an effective tool for profiling mucin glyco-epitopes and highlights their potential for discovery of biologically important motifs in bacterial-host interactions and fertility...
  97. ncbi Cytokines and growth factors in mostly atherosclerotic patients on hemodialysis determined by biochip array technology
    Jadranka Sertic
    Department of Clinical Chemistry, Zagreb University School of Medicine, Zagreb, Croatia
    Clin Chem Lab Med 45:1347-52. 2007
    ....
  98. pmc Protein array profiling of tic patient sera reveals a broad range and enhanced immune response against Group A Streptococcus antigens
    Mauro Bombaci
    Novartis Vaccines and Diagnostics, Siena, Italy
    PLoS ONE 4:e6332. 2009
    ..This may be relevant in the context of one of the hypothesis proposing that GAS antigen-dependent induction of autoantibodies in susceptible individuals may be involved the occurrence of tic disorders...
  99. ncbi Attomolar protein detection in complex sample matrices with semi-homogeneous fluidic force discrimination assays
    S P Mulvaney
    Naval Research Laboratory, Washington, DC 20375, USA
    Biosens Bioelectron 24:1109-15. 2009
    ....
  100. pmc Analysis of glycans on serum proteins using antibody microarrays
    Songming Chen
    Van Andel Research Institute, Grand Rapids, MI, USA
    Methods Mol Biol 520:39-58. 2009
    ..We describe those methods along with the methods for preparing and treating serum samples, running the experiments, and designing and interpreting the experiments...
  101. ncbi Protein microarrays with carbon nanotubes as multicolor Raman labels
    Zhuo Chen
    Department of Chemistry and Laboratory for Advanced Materials, Stanford University, 333 Campus Drive, Mudd Building, Room 121, Stanford, California 94305, USA
    Nat Biotechnol 26:1285-92. 2008
    ..SWNT Raman tags are not subject to photobleaching or quenching. By conjugating different antibodies to pure (12)C and (13)C SWNT isotopes, we demonstrate multiplexed two-color SWNT Raman-based protein detection...

Research Grants65

  1. Screening Complete TB Proteome for Protective Antigens
    Alan Greener; Fiscal Year: 2010
    ....
  2. Seriodiagnostic arrays for biodefense and emerging infectious diseases
    DAVID HUW DAVIES; Fiscal Year: 2010
    ....
  3. Energy Restrction and Mammary Cancer
    Henry J Thompson; Fiscal Year: 2013
    ....
  4. Proteome-wide screen for M. tuberculosis antigens
    Robert N Husson; Fiscal Year: 2010
    ..Earlier, simpler, and more sensitive and specific TB diagnosis could have a major impact on the identification and treatment of people with TB in the U.S. and worldwide. ..
  5. MALDI-TOF/TOF MS TO SUPPORT BIOMEDICAL RESEARCH
    Catherine E Costello; Fiscal Year: 2012
    ..The proposed instrument system will accelerate progress towards diagnosis and treatment based on understanding the mechanisms of these diseases and conditions and will thus contribute directly toward improving human health. ..
  6. A protein array platform for anti-citrulline antibodies in Rheumatoid Arthritis
    Ji Qiu; Fiscal Year: 2013
    ..Furthermore, what we propose to develop can be applied to studies in understanding the biology of other post-translational modifications. ..
  7. Development of Novel Inducers of Non-Apoptotic Cell Death to Target Glioblastoma
    William A Maltese; Fiscal Year: 2013
    ..In addition, the development of tumor-homing NP or prodrugs that can be targeted to GBM would represent a technological advance that might be applied more generally for delivery of other therapeutic agents. ..
  8. Biosignature of the endometrium in women with endometriosis
    Neil Sidell; Fiscal Year: 2010
    ....
  9. IDO-silencing Salmonella therapy for the treatment of primary and metastatic PDAC
    Don J Diamond; Fiscal Year: 2013
    ..Cytokines involved in PMN recruitment will be evaluated using protein array analysis and flow cytometry of PDAC tumors from mice treated with shIDO-ST versus scrambled sequence control (shScr-..
  10. Amino Acid Regulation of Alternative Splicing
    Michael S Kilberg; Fiscal Year: 2013
    ..The insight gained from these studies will impact the fields of: 1) macro-nutrient control of pre- mRNA alternative splicing;2) amino acid-dependent control of transcription;and 3) ATF3 function in nutrition and disease. ..
  11. Cold Spring Harbor Laboratory Course on Proteomics
    David J Stewart; Fiscal Year: 2013
    ....
  12. Antibody Biomarkers for Early Detection of Tuberculosis
    D Mitchell Magee; Fiscal Year: 2012
    ..The successful completion of this program will provide new and improved strategies for detecting TB rapidly and sensitively, in formats that can be applied in all settings globally. ..
  13. Integrating cellular and molecular heterogeneity of TKI response in NSCLC cells
    PETER LEE FRICK; Fiscal Year: 2013
    ..This knowledge would help reconcile the promise of targeted therapies with the challenge of tumor heterogeneity, and could have high translational value. ..
  14. Piezoelectric Pipetting for High Density Nucleic Acid Programmable Protein Arrays
    PETER J WIKTOR; Fiscal Year: 2013
    ..This grant will develop technology to mass produce better and less expensive protein microarrays, making them more readily accessible to the broad research and health care communities. ..
  15. Piezoelectric Pipetting for High Density Nucleic Acid Programmable Protein Arrays
    PETER J WIKTOR; Fiscal Year: 2011
    ..This grant will develop technology to mass produce better and less expensive protein microarrays, making them more readily accessible to the broad research and health care communities. ..
  16. Targeted and global proteomic strategies for early breast cancer detection
    Susan J Fisher; Fiscal Year: 2011
    ..abstract_text> ..
  17. Development of Biomarkers for IBD Using Protein Chips
    Xuhang Li; Fiscal Year: 2010
    ..Moreover, identification of IBD-specific antibodies might not only reveal new pathogenic mechanisms of IBD, but also provide potential molecular targets for therapeutic intervention of IBD. ..
  18. A Novel single-tier Lyme disease assay using Borrelia burgdorferi Protein Arrays.
    Benjamin J Luft; Fiscal Year: 2012
    ..This will ultimately allow the development of an office test which will facilitate the quick initiation of therapy which is associated with cure. ..
  19. ACTIVITY-BASED KINASE DISCOVERY IN SMALL CELL LUNG CANCER
    Eric B Haura; Fiscal Year: 2013
    ..Aim 4 will interrogate the functional significance of ABPP identified proteins kinases using RNAi and small molecule inhibitors to better guide biomarker selection. ..
  20. Cockayne syndrome: role of the innate immune response in neurodegeneration
    Alan M Weiner; Fiscal Year: 2013
    ....
  21. Discovery Platform for Cancer Antigens
    Kevin P Claffey; Fiscal Year: 2012
    ..Finally, these reagents will be made available to the broad cancer research community to rapidly define their utility and application in multiple diagnostic and clinical therapeutic approaches. ..
  22. The PI3K/PTEN/AKT Signal Transduction Cascade in Breast Cancer
    Ana M Gonzalez Angulo; Fiscal Year: 2010
    ..The outcome of this project is expected to position me to submit a competitive R01 application during the fourth year of the proposed award. ..
  23. The Role of the PI3K-AKT Pathway in Melanoma Brain Metastasis
    Michael Davies; Fiscal Year: 2013
    ..This project may lead to new therapies to prevent and/or treat melanoma brain metastases, and may suggest if similar approaches will benefit patients with other types of cancer. ..
  24. MAPP Research Networdk TIssue Analysis &Technology Core
    M S Lucia; Fiscal Year: 2012
    ..The establishment of a Tissue Analysis and Technology Core Facility is crucial to the success of this research program. ..
  25. Fluorescence Lifetime Imaging Microscopy (FLIM)
    Joseph R Lakowicz; Fiscal Year: 2010
    ..Indeed, providing access to state-of-the-art instrumentation is a key aspect of the training mission at UMB. The new ITC will enhance the training of students supported by a number of NIH training grants. ..
  26. TARGETING REGULATORS OF APOPTOSIS IN AML
    MICHAEL W ANDREEFF; Fiscal Year: 2011
    ..If successful, these studies will provide rationale for greatly improved therapies for AML that target both, the leukemic cells and their microenvironment. ..
  27. Ras-mediated erbB signaling in Osteosarcoma
    Dennis P Hughes; Fiscal Year: 2010
    ....
  28. Computational methods for molecular tomography
    Hanspeter Winkler; Fiscal Year: 2011
    ..This will ensure that our methods are readily available in other areas of biological research. ..
  29. Random Display of Gut Microflora Proteins to Analyze Obesity
    Douglas M Fowler; Fiscal Year: 2010
    ..This proposal describes a novel technology that should discover why certain types of gut bacteria are associated with obesity. A better understanding of this potential cause of obesity could lead to an effective treatment. ..
  30. Developing functional membrane protein arrays for highly efficient drug screening
    Xichun Zhou; Fiscal Year: 2011
    ..The membrane protein arrays will be useful in the development of effective and safe therapeutic agents for the treatment of wide range diseases such as cancer, diabetes, inflammation, and cardiac dysfunction. ..
  31. A Rational Design of a Platform for de novo Gene Synthesis
    Hendrik J Viljoen; Fiscal Year: 2010
    ..The combination of rapid automated gene synthesis with proteomics may accelerate the development of new disease markers and drug targets. ..
  32. Harnessing p27 for Prognostication of Pediatric Osteosarcoma
    Tsz Kwong Man; Fiscal Year: 2013
    ..Completion of the proposed study is a critical step to achieve this goal. Furthermore, since p27 has been implicated in other cancers, the results obtained from this study will have a larger impact on the field of cancer biomarkers. ..
  33. Nonparametric Analysis of Reverse-Phase Protein Lysate Array Data
    JIANHUA contact HU; Fiscal Year: 2010
    ..These tools are needed to support better applications of protein lysate array technology in clinical and biomedical research. ..
  34. Human Proteome Arrays for Auto-Antibody Identification in Clinical Cancer Studies
    John H Kenten; Fiscal Year: 2011
    ..abstract_text> ..
  35. Chemoprevention of Peutz-Jeghers Polyposis in a Mouse Model by Niclosamide
    Chongjuan Wei; Fiscal Year: 2012
    ....
  36. SURFACE ANALYSIS FACILITY FOR BIOMEDICAL PROBLEMS
    David G Castner; Fiscal Year: 2013
    ..Dissemination (presentations, publications, website, etc.) and Training projects (workshops, courses, one-on-one instruction, etc.) are also key parts of the NESAC/BIO program. . ..
  37. Notch and stem cells in lung cancer treated with erlotinib plus notch inhibitor.
    DON LYNN GIBBONS; Fiscal Year: 2012
    ..While erlotinib is less effective vs EGFR wild type tumors, therapeutic benefit is nevertheless seen. ..
  38. Submerged Printing of Lipid and Membrane Protein Arrays
    Paul O Neilsen; Fiscal Year: 2012
    ....
  39. Targeting the PI3K/Akt pathway in High Grade Glioma
    Anna Joy; Fiscal Year: 2013
    ..We will also find ways to identify tumor subgroups that use different arms of the Akt pathway and understand which proteins and pathways are active in tumor subgroups. ..
  40. MPSA Pathology Coordinating Center
    M Lucia; Fiscal Year: 2004
    ..abstract_text> ..
  41. Platform for Massively Parallel Selection of Aptamer Ligands
    George W Jackson; Fiscal Year: 2012
    ..The developed ligands can then be further characterized as promising drug candidates, diagnostic labels, and other research applications perhaps eventually including personalized medicine. ..
  42. A Nanotechnology Enabled Point of Care Diagnostic Platform for Early Stage Cancer
    James Nelson; Fiscal Year: 2009
    ..We propose to develop a nanotechnology enabled protein signature based diagnostic test for prostate cancer available at the point of primary care. ..
  43. Self-Assembling Protein Microchips
    ANDREW OLEINIKOV; Fiscal Year: 2002
    ..These chips can be used for diagnostic analysis, drug-lead screening, and functional genomics studies including protein-protein and protein-target interactions, catalytic and inhibitory activity. ..
  44. A Novel Method for Saliva Proteomic Analysis
    Gang Peng; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  45. Protein arrays to profile cytokine expression in ovaria*
    Ruo Pan Huang; Fiscal Year: 2005
    ..The data will be analyzed using statistics and bioinformatics tool and correlated with pathological and clinical data. The successful outcome of this grant may impact on the development of new biomarkers for patient-care management. ..
  46. Prostate Cancer Prognosis by FFPE Tissue Proteomics
    David Krizman; Fiscal Year: 2004
    ..2) Demonstrate the capability of Liquid Tissue TM protein array analysis to provide for molecular distinctions between early, moderate, and high Gleason grades of prostate cancers ..
  47. MAPPING VACULAR DIVERSITY OF HUMAN CANCER
    Wadih Arap; Fiscal Year: 2006
    ..We expect that the striking molecular diversity of blood vessels will be translated into clinical applications. If successful, our efforts will accelerate the development of targeted anticancer therapies. [unreadable] [unreadable]..
  48. Multiplex Microarray for the Detection of Pre-Type 1 Diabetes Autoantibodies
    ZUHEIR AWDEH; Fiscal Year: 2009
    ..A multiplex microarray methodology that would allow testing for numerous significant T1D autoantibodies should also allow for early identification of asymptomatic individuals and provide better chances of preventing disease. ..
  49. Site-directed Covalently Bound Fusion Protein Microarray
    Bryce Nelson; Fiscal Year: 2004
    ..abstract_text> ..
  50. Proteomics Approaches to Interstitial Cystitis.
    Brian Liu; Fiscal Year: 2006
    ..Through these specific aims, our goal is to fulfill the need to develop reliable predictive and diagnostic tools for IC, which is deemed to be a high-priority by the NIDDK Bladder Research Progress Group. ..
  51. RETINAL DEGENERATION: MOLECULAR AND BIOCHEMICAL ASPECTS
    MUAYYAD AL UBAIDI; Fiscal Year: 2006
    ..Uncovering these genes will lend itself to understanding the initiation and execution of retinal apoptosis in degenerative disorders and serves to enhance our understanding of normal age-related cell death. ..
  52. Microsphere Coatings for Protein Arrays
    PATRICK GUIRE; Fiscal Year: 2007
    ..Additionally, the improved microarrays will benefit researchers studying protein-protein interactions which may ultimately greatly aid in disease management. [unreadable] [unreadable] [unreadable]..
  53. Modulation of cytokine networks in mycoplasma pneumonia
    Jerry Simecka; Fiscal Year: 2004
    ..abstract_text> ..
  54. PEPTIDE FOR GENTLE PURIFICATION OF BIOTINYLATED PROTEIN
    Ronald Gill; Fiscal Year: 2001
    ..proposed commercial application: not available ..
  55. A Rational Design of a Platform for de novo Gene Synthesis
    Hendrik Viljoen; Fiscal Year: 2007
    ..The combination of rapid automated gene synthesis with proteomics may accelerate the development of new disease markers and drug targets. ..
  56. Adaptive immunity of P. aeruginosa in cystic fibrosis
    GREGORY PRIEBE; Fiscal Year: 2006
    ..Knowledge gained from these studies will elucidate new strategies for the prevention and treatment of P. aeruginosa infections in the setting of CF. [unreadable] [unreadable] [unreadable]..
  57. Self-immobilizing Proteins
    Russell Stewart; Fiscal Year: 2005
    ..The widely expected future impact of protein arrays on clinical diagnosis and other areas of human health care may be realized more quickly with new and effective protein modification technology. ..
  58. Protein arrays for screening novel ligands for nuclear hormone receptors.
    Hui Ge; Fiscal Year: 2006
    ..This research will provide a rapid method of screening new compounds for nuclear hormone receptor binding and determining specificity of that binding. [unreadable] [unreadable] [unreadable]..
  59. Llama-derived phage display antibody arrays for FSHD
    Silvere van der Maarel; Fiscal Year: 2003
    ..Evidently, these antibodies can also be used individually for specific immunohistochemical and immunocytochemical studies. ..
  60. Selective patterning of biomolecules into protein arrays
    Karen Christman; Fiscal Year: 2006
    ..This technology may also be applied to the detection and diagnosis of a variety of other diseases. [unreadable] [unreadable]..
  61. Spectral Imaging System for Encoded Particle Arrays
    David Kelso; Fiscal Year: 2005
    ..In the latter phases of our proposed research, we plan on extending the method to particles sedimented in microtiter wells and demonstrate the feasibility of detecting proteins bound to QD-encoded particles. ..
  62. Novel Protein Array Based on Binary Coded Microchips
    Herve Le Calvez; Fiscal Year: 2001
    ..Both the equipment and detection method software is expected to be significantly cheaper than the current systems. The protein chip commercial field is as large as the DNA array field. ..
  63. Protein Expression in Failing Human Hearts
    Margaret V Westfall; Fiscal Year: 2010
    ..These experiments will provide fundamental information needed to test new hypotheses in animal models of heart failure, and design future therapeutic targets for treating heart failure. ..
  64. CODA Assembly of Mutant Genes
    She pin Hung; Fiscal Year: 2005
    ..abstract_text> ..
  65. A Metal-Mediated Approach To Protein Immobilization
    CHRISTOPHER BIELAWSKI; Fiscal Year: 2004
    ..In a broader sense, the proposed research attempts to integrate the strongest attributes of organometallic chemistry, materials science, and biology. ..