enzyme linked immunosorbent assay

Summary

Summary: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

Top Publications

  1. ncbi Immunization with amyloid-beta attenuates Alzheimer-disease-like pathology in the PDAPP mouse
    D Schenk
    Elan Pharmaceuticals, South San Francisco, California 94080, USA
    Nature 400:173-7. 1999
  2. ncbi High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever
    Daniel H Libraty
    Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, 55 Lake Avenue N, Worcester, MA 01655, USA
    J Infect Dis 186:1165-8. 2002
  3. pmc Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia
    Sharon E Maynard
    Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115, USA
    J Clin Invest 111:649-58. 2003
  4. ncbi A beta peptide vaccination prevents memory loss in an animal model of Alzheimer's disease
    D Morgan
    Department of Pharmacology, University of South Florida, Tampa 33612, USA
    Nature 408:982-5. 2000
  5. ncbi Ccr2 deficiency impairs microglial accumulation and accelerates progression of Alzheimer-like disease
    Joseph El Khoury
    Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129, USA
    Nat Med 13:432-8. 2007
  6. ncbi Autoantibodies in breast cancer: their use as an aid to early diagnosis
    C Chapman
    Division of Breast Surgery, The University of Nottingham, Nottingham, UK
    Ann Oncol 18:868-73. 2007
  7. pmc Nature of nonfunctional envelope proteins on the surface of human immunodeficiency virus type 1
    Penny L Moore
    Torrey Pines Institute for Molecular Studies, San Diego, CA 92121, USA
    J Virol 80:2515-28. 2006
  8. pmc High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes
    U Andersson
    Department of Medicine, Rheumatology Unit, Karolinska Hospital, 17176 Stockholm, Sweden
    J Exp Med 192:565-70. 2000
  9. ncbi Human antibodies to recombinant protein constructs of Plasmodium falciparum Apical Membrane Antigen 1 (AMA1) and their associations with protection from malaria
    Spencer D Polley
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Vaccine 23:718-28. 2004
  10. doi Dectin-1 directs T helper cell differentiation by controlling noncanonical NF-kappaB activation through Raf-1 and Syk
    Sonja I Gringhuis
    Department of Molecular Cell Biology and Immunology, Vrije University Medical Center, 1007 MC Amsterdam, The Netherlands
    Nat Immunol 10:203-13. 2009

Detail Information

Publications414 found, 100 shown here

  1. ncbi Immunization with amyloid-beta attenuates Alzheimer-disease-like pathology in the PDAPP mouse
    D Schenk
    Elan Pharmaceuticals, South San Francisco, California 94080, USA
    Nature 400:173-7. 1999
    ..Our results raise the possibility that immunization with amyloid-beta may be effective in preventing and treating Alzheimer's disease...
  2. ncbi High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever
    Daniel H Libraty
    Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, 55 Lake Avenue N, Worcester, MA 01655, USA
    J Infect Dis 186:1165-8. 2002
    ..An elevated free sNS1 level (> or =600 ng/mL) within 72 h of illness onset identified patients at risk for developing DHF...
  3. pmc Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia
    Sharon E Maynard
    Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115, USA
    J Clin Invest 111:649-58. 2003
    ..These observations suggest that excess circulating sFlt1 contributes to the pathogenesis of preeclampsia...
  4. ncbi A beta peptide vaccination prevents memory loss in an animal model of Alzheimer's disease
    D Morgan
    Department of Pharmacology, University of South Florida, Tampa 33612, USA
    Nature 408:982-5. 2000
    ..The A beta-vaccinated mice also had a partial reduction in amyloid burden at the end of the study. This therapeutic approach may thus prevent and, possibly, treat Alzheimer's dementia...
  5. ncbi Ccr2 deficiency impairs microglial accumulation and accelerates progression of Alzheimer-like disease
    Joseph El Khoury
    Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129, USA
    Nat Med 13:432-8. 2007
    ..Thus, Ccr2-dependent microglial accumulation plays a protective role in the early stages of Alzheimer disease by promoting Abeta clearance...
  6. ncbi Autoantibodies in breast cancer: their use as an aid to early diagnosis
    C Chapman
    Division of Breast Surgery, The University of Nottingham, Nottingham, UK
    Ann Oncol 18:868-73. 2007
    ..There is increasing evidence that the immune system produces a humoral response to cancer-derived antigens. This study assessed the diagnostic potential of autoantibodies to multiple known tumour-associated proteins...
  7. pmc Nature of nonfunctional envelope proteins on the surface of human immunodeficiency virus type 1
    Penny L Moore
    Torrey Pines Institute for Molecular Studies, San Diego, CA 92121, USA
    J Virol 80:2515-28. 2006
    ..We hypothesize that these nonfunctional forms of Env on particle surfaces serve to divert the antibody response, helping the virus to evade neutralization...
  8. pmc High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes
    U Andersson
    Department of Medicine, Rheumatology Unit, Karolinska Hospital, 17176 Stockholm, Sweden
    J Exp Med 192:565-70. 2000
    ..Together, these results indicate that, like other cytokine mediators of endotoxin lethality (e.g., TNF and IL-1), extracellular HMG-1 is a regulator of monocyte proinflammatory cytokine synthesis...
  9. ncbi Human antibodies to recombinant protein constructs of Plasmodium falciparum Apical Membrane Antigen 1 (AMA1) and their associations with protection from malaria
    Spencer D Polley
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Vaccine 23:718-28. 2004
    ..Results encourage the development of an AMA1 vaccine based on the full-length ectodomain, and indicate that the function of human antibodies to allele-specific and conserved epitopes in AMA1 should be studied further...
  10. doi Dectin-1 directs T helper cell differentiation by controlling noncanonical NF-kappaB activation through Raf-1 and Syk
    Sonja I Gringhuis
    Department of Molecular Cell Biology and Immunology, Vrije University Medical Center, 1007 MC Amsterdam, The Netherlands
    Nat Immunol 10:203-13. 2009
    ..Thus, dectin-1 activates two independent signaling pathways, one through Syk and one through Raf-1, to induce immune responses...
  11. pmc Phase 1/2a study of the malaria vaccine candidate apical membrane antigen-1 (AMA-1) administered in adjuvant system AS01B or AS02A
    Michele D Spring
    United States Military Malaria Vaccine Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 4:e5254. 2009
    ....
  12. ncbi Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1
    Daniel J Ceradini
    Laboratory of Microvascular Research and Vascular Tissue Engineering, Institute of Reconstructive Plastic Surgery, New York University School of Medicine, New York, New York 10016, USA
    Nat Med 10:858-64. 2004
    ..These data show that the recruitment of CXCR4-positive progenitor cells to regenerating tissues is mediated by hypoxic gradients via HIF-1-induced expression of SDF-1...
  13. ncbi Low plasma concentrations of interleukin 10 in severe malarial anaemia compared with cerebral and uncomplicated malaria
    J A Kurtzhals
    Immunology Unit, Noguchi Memorial Institute for Medical Research, Legon, Ghana
    Lancet 351:1768-72. 1998
    ..Conversely, interleukin 10 (IL-10), which mediates feed-back regulation of TNF, stimulates bone-marrow function in vitro and counteracts anaemia in mice. We investigated the associations of these cytokines with malarial anaemia...
  14. pmc Cohort study of the association of antibody levels to AMA1, MSP119, MSP3 and GLURP with protection from clinical malaria in Ghanaian children
    Daniel Dodoo
    Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
    Malar J 7:142. 2008
    ..This study was aimed at assessing in a standardized manner the relationship between the antibody responses to four malaria vaccine candidate antigens and protection from clinical malaria, in a cohort of Ghanaian children...
  15. ncbi High-affinity binders selected from designed ankyrin repeat protein libraries
    H Kaspar Binz
    Biochemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Nat Biotechnol 22:575-82. 2004
    ..Thus, our AR protein libraries are valuable sources for binding molecules and, because of the very favorable biophysical properties of the designed AR proteins, an attractive alternative to antibody libraries...
  16. pmc Development and characterization of a standardized ELISA including a reference serum on each plate to detect antibodies induced by experimental malaria vaccines
    Kazutoyo Miura
    Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    Vaccine 26:193-200. 2008
    b>Enzyme linked immunosorbent assay (ELISA) has been widely used to measure antibody titers for evaluating the immunogenicity of a vaccine...
  17. ncbi Dengue diagnosis, advances and challenges
    Maria G Guzman
    Virology Department, PAHO WHO Collaborating Center for Viral Diseases, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodi a, Km 6, Ciudad Habana, Cuba
    Int J Infect Dis 8:69-80. 2004
    ....
  18. pmc Cerebrospinal fluid and serum biomarkers of cerebral malaria mortality in Ghanaian children
    Henry B Armah
    Morehouse School of Medicine, Department of Microbiology, Biochemistry and Immunology, Atlanta, Georgia, USA
    Malar J 6:147. 2007
    ..Identification of prognostic markers that can predict CM severity will enable development of better intervention...
  19. ncbi Complexity of the malaria vectorial system in Cameroon: contribution of secondary vectors to malaria transmission
    Christophe Antonio-Nkondjio
    Laboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la Lutte Contre les Endemies en Afrique Centrale, P O Box 288, Yaounde, Cameroon
    J Med Entomol 43:1215-21. 2006
    ..Both species have strong exophilic and/or exophagic habits such that they might elude vector control directed against endophilic and endophagic malaria vectors...
  20. ncbi Clinical, epidemiologic, and virologic features of dengue in the 1998 epidemic in Nicaragua
    E Harris
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley 94720 7360, USA
    Am J Trop Med Hyg 63:5-11. 2000
    ..As evidenced by the analysis of this epidemic, the epidemiology of dengue can differ according to geographic region and viral serotype...
  21. pmc Isolation of genetically diverse Marburg viruses from Egyptian fruit bats
    Jonathan S Towner
    Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA
    PLoS Pathog 5:e1000536. 2009
    ..These data indicate common Egyptian fruit bats can represent a major natural reservoir and source of Marburg virus with potential for spillover into humans...
  22. pmc The role of CXCR4 in maintaining peripheral B cell compartments and humoral immunity
    Yuchun Nie
    Dept of Microbiology, Columbia University, 701 West 168th St, HHSC 1406, New York, NY 10032, USA
    J Exp Med 200:1145-56. 2004
    ..These findings establish an important role for CXCR4 in regulating homeostasis of B cell compartmentalization and humoral immunity...
  23. pmc Histidine-rich protein II: a novel approach to malaria drug sensitivity testing
    Harald Noedl
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences USAMC AFRIMS, Bangkok, Thailand
    Antimicrob Agents Chemother 46:1658-64. 2002
    ..Standard operating procedures, updated information, and analytical software are available from http://malaria.farch.net...
  24. ncbi Microglial activation with atypical proinflammatory cytokine expression in a rat model of Parkinson's disease
    Amaicha M Depino
    Institute Leloir Foundation CONICET University of Buenos Aires, Avenue Patricias Argentinas 435, 1405 Buenos Aires, Argentina
    Eur J Neurosci 18:2731-42. 2003
    ..The production of proinflammatory cytokines from "primed" microglia may in turn modulate disease progression as has been recently proposed in a model of prion disease...
  25. ncbi The serum level of soluble urokinase receptor is elevated in tuberculosis patients and predicts mortality during treatment: a community study from Guinea-Bissau
    J Eugen-Olsen
    Clinical Research Unit, Hvidovre Hospital, Denmark
    Int J Tuberc Lung Dis 6:686-92. 2002
    ..To investigate whether the serum level of soluble urokinase plasminogen activator receptor (suPAR) carries prognostic information in individuals infected with Mycobacterium tuberculosis...
  26. pmc Newly discovered ebola virus associated with hemorrhagic fever outbreak in Uganda
    Jonathan S Towner
    Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
    PLoS Pathog 4:e1000212. 2008
    ....
  27. ncbi Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery
    Jaya Mishra
    Division of Nephrology and Hypertension, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229 3039, USA
    Lancet 365:1231-8. 2005
    ..We tested the hypothesis that neutrophil gelatinase-associated lipocalin (NGAL) is an early biomarker for ischaemic renal injury after cardiopulmonary bypass...
  28. ncbi Immunogenicity and protective efficacy of Escherichia coli expressed Plasmodium falciparum merozoite surface protein-1(42) using human compatible adjuvants
    Suraksha Sachdeva
    International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
    Vaccine 24:2007-16. 2006
    ..berghei/P. falciparum chimeric line (Pb-PfM19) that expresses Plasmodium falciparum MSP-1(19). These findings may be useful for the development of a malaria vaccine based on Plasmodium falciparum MSP-1(42)...
  29. pmc Toll-like receptor 9 mediates innate immune activation by the malaria pigment hemozoin
    Cevayir Coban
    Exploratory Research for Advanced Technology ERATO, Japan Science and Technology Agency JST, Osaka, Japan
    J Exp Med 201:19-25. 2005
    ..These data suggest that TLR9-mediated, MyD88-dependent, and CQ-sensitive innate immune activation by HZ may play an important role in malaria parasite-host interactions...
  30. pmc The detection of antibodies against peste des petits ruminants virus in cattle, sheep and goats and the possible implications to rinderpest control programmes
    J Anderson
    ARFC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK
    Epidemiol Infect 112:225-31. 1994
    ....
  31. ncbi Urokinase plasminogen activator: a prognostic marker in breast cancer including patients with axillary node-negative disease
    M J Duffy
    Department of Nuclear Medicine, St Vincent s Hospital, Dublin, Ireland
    Clin Chem 44:1177-83. 1998
    ..Measurement of uPA concentrations might thus be of value in selecting the more aggressive subpopulation of node-negative breast cancer patients that could benefit from adjuvant therapy...
  32. pmc Broadly neutralizing antibodies targeted to the membrane-proximal external region of human immunodeficiency virus type 1 glycoprotein gp41
    M B Zwick
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Virol 75:10892-905. 2001
    ..g., subtypes B, C, and E). The results suggest that a rather extensive region of gp41 close to the transmembrane domain is accessible to neutralizing Abs and could form a useful target for vaccine design...
  33. ncbi High plasma levels of intact and cleaved soluble urokinase receptor reflect immune activation and are independent predictors of mortality in HIV-1-infected patients
    Sisse Rye Ostrowski
    Department of Infectious Diseases, Rigshospitalet, Copenhagen, DK 2100 Denmark
    J Acquir Immune Defic Syndr 39:23-31. 2005
    ..This study investigated plasma levels of suPAR(I-III), suPAR(II-III), and 1-domain suPAR [suPAR(I)] and their predictive value for survival in HIV patients...
  34. pmc An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients
    P R Young
    Sir Albert Sakzewski Virus Research Centre, The Royal Children s Hospital, Herston, Brisbane 4029, Australia
    J Clin Microbiol 38:1053-7. 2000
    ....
  35. pmc An outbreak of Rift Valley fever in Northeastern Kenya, 1997-98
    Christopher W Woods
    Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Emerg Infect Dis 8:138-44. 2002
    ..Public education during epizootics may reduce human illness and deaths associated with future outbreaks...
  36. ncbi Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos
    Stuart D Blacksell
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR
    Diagn Microbiol Infect Dis 60:43-9. 2008
    ....
  37. pmc Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections
    D A Martin
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80522, USA
    J Clin Microbiol 38:1823-6. 2000
    ..0 as a positive cutoff value. With a blind-coded human serum panel, this combined MAC-ELISA was shown to have test sensitivity and specificity that correlated well with those of other serological techniques...
  38. ncbi A potent cross-clade neutralizing human monoclonal antibody against a novel epitope on gp41 of human immunodeficiency virus type 1
    G Stiegler
    Institute of Applied Microbiology, University of Agricultural Sciences, A 1190 Vienna, Austria
    AIDS Res Hum Retroviruses 17:1757-65. 2001
    ..Moreover, our results suggest that 4E10 should be further investigated for passive anti-HIV immunotherapy...
  39. ncbi Alternative macrophage activation is essential for survival during schistosomiasis and downmodulates T helper 1 responses and immunopathology
    De Broski R Herbert
    University of Cape Town, Health Sciences Faculty, 7925 Cape Town, South Africa
    Immunity 20:623-35. 2004
    ..Together, this shows that alternative macrophages are essential during schistosomiasis for protection against organ injury through downregulation of egg-induced inflammation...
  40. ncbi IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge
    Shabaana A Khader
    Trudeau Institute, Saranac Lake, New York 12983, USA
    Nat Immunol 8:369-77. 2007
    ....
  41. ncbi Monoclonal antibodies to plant cell wall xylans and arabinoxylans
    Lesley McCartney
    University of Leeds, Centre for Plant Sciences, Faculty of Biological Sciences, Leeds, LS2 9JT, United Kingdom
    J Histochem Cytochem 53:543-6. 2005
    ..Immunocytochemical analyses indicated the presence of both epitopes in secondary cell walls of xylem but differences in occurrence in other cell types...
  42. ncbi Risk factors associated with asymptomatic infection by Leishmania chagasi in north-east Brazil
    A J M Caldas
    Federal University of Maranhao, Departamento de Patologia, Praça Madre Deus, No 2, Madre Deus, 65 025 560, São Luis Maranhão, Brazil
    Trans R Soc Trop Med Hyg 96:21-8. 2002
    ..Malnutrition was not found to be associated with infection...
  43. pmc Use of serological assays for diagnosis of hepatitis E virus genotype 1 and 3 infections in a setting of low endemicity
    M Herremans
    National Institute for Public Health and the Environment, RIVM, Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, P O Box 1, 3720 BA Bilthoven, The Netherlands
    Clin Vaccine Immunol 14:562-8. 2007
    ..We conclude that a combination of ELISA and immunoblotting is needed for acceptable specificity and sensitivity of HEV assays under conditions of low endemicity...
  44. ncbi Exploring sources of heterogeneity in systematic reviews of diagnostic tests
    Jeroen G Lijmer
    Department of Clinical Epidemiology and Biostatistics, Academic Medical Centre, University of Amsterdam, The Netherlands
    Stat Med 21:1525-37. 2002
    ..Results of such analyses will help understand problems with the transferability of diagnostic tests and to point out flaws in primary studies. As such, they can guide the design of future studies...
  45. ncbi Serum level of soluble urokinase-type plasminogen activator receptor is a strong and independent predictor of survival in human immunodeficiency virus infection
    N Sidenius
    Department of Molecular Pathology and Medicine, Molecular Genetics Unit, DIBIT, San Raffaele Scientific Institute, Milan, Italy
    Blood 96:4091-5. 2000
    ..This report is the first to demonstrate an important association between the plasminogen activator system and disease progression in HIV-1 infection...
  46. ncbi Sulfasalazine and BAY 11-7082 interfere with the nuclear factor-kappa B and I kappa B kinase pathway to regulate the release of proinflammatory cytokines from human adipose tissue and skeletal muscle in vitro
    Martha Lappas
    Department of Obstetrics and Gynecology, University of Melbourne and Mercy Perinatal Research Center, Mercy Hospital for Women, 126 Clarendon Street, East Melbourne, 3002 Victoria, Australia
    Endocrinology 146:1491-7. 2005
    ..Control of the IKK-beta/NF-kappa B pathway may therefore provide an alternative therapeutic strategy for regulating aberrant cytokine release and thereby alleviating insulin resistance in type 2 diabetes mellitus...
  47. pmc Preclinical assessment of the receptor-binding domain of Plasmodium vivax Duffy-binding protein as a vaccine candidate in rhesus macaques
    A Moreno
    Emory Vaccine Center and Yerkes National Primate Research Center, Emory University, 954 Gatewood Road, Atlanta, GA 30329, USA
    Vaccine 26:4338-44. 2008
    ..These results provide support for further clinical development of a vaccine for P. vivax malaria based on recombinant PvRII...
  48. ncbi Hodgkin/Reed-Sternberg cells induce fibroblasts to secrete eotaxin, a potent chemoattractant for T cells and eosinophils
    F Jundt
    Universitatsklinikum Charite, Robert Rossle Klinik, Humboldt University of Berlin, Berlin, Germany
    Blood 94:2065-71. 1999
    ..Our data suggest that eotaxin is involved in the pathobiology of Hodgkin's disease by contributing to eosinophil and T-lymphocyte recruitment...
  49. ncbi Correlation between direct ELISA, single epitope-based inhibition ELISA and pseudovirion-based neutralization assay for measuring anti-HPV-16 and anti-HPV-18 antibody response after vaccination with the AS04-adjuvanted HPV-16/18 cervical cancer vaccine
    Francis J Dessy
    Research and Development, GlaxoSmithKline Biologicals, Rixensart, Belgium
    Hum Vaccin 4:425-34. 2008
    ..4 years) and the age of the vaccine recipient...
  50. pmc Biomarkers of acute kidney injury
    Vishal S Vaidya
    Renal Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Annu Rev Pharmacol Toxicol 48:463-93. 2008
    ....
  51. ncbi Results of a tuberculosis-specific IFN-gamma assay in children at high risk for tuberculosis infection
    S J Tsiouris
    Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY 10032, USA
    Int J Tuberc Lung Dis 10:939-41. 2006
    ..001). If studies confirm that this difference is due to higher specificity of this IGRA, it may have an important role to play in the diagnosis of LTBI and identification of children at true risk for TB...
  52. ncbi Distinct immunity in patients with visceral leishmaniasis from that in subclinically infected and drug-cured people: implications for the mechanism underlying drug cure
    A Hailu
    Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia
    J Infect Dis 184:112-5. 2001
    ..These findings may have implications for the immunologic mechanism underlying drug cure in visceral leishmaniasis...
  53. ncbi Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax
    Mayara B Barbedo
    Departamento de Analises Clinicas e Toxicologicas, Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo, Súo Paulo, Brasil
    Mem Inst Oswaldo Cruz 102:335-9. 2007
    ..The reason for that is presently unknown but it may contribute to the high risk of re-infection in individuals living in the endemic areas...
  54. ncbi Puumala and Dobrava viruses cause hemorrhagic fever with renal syndrome in Bosnia-Herzegovina: evidence of highly cross-neutralizing antibody responses in early patient sera
    A Lundkvist
    Swedish Institute for Infectious Disease Control, Stockholm
    J Med Virol 53:51-9. 1997
    ..In conclusion, the results revealed that both Puumala- and Dobrava-like viruses caused HFRS in Bosnia-Herzegovina, whereas no signs of Hantaan or Seoul virus involvement were found...
  55. pmc Fibroblast growth factor-21 is induced in human skeletal muscles by hyperinsulinemia
    Pernille Hojman
    Department of Infectious Diseases, Centre of Inflammation and Metabolism, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Diabetes 58:2797-801. 2009
    ..Recently, FGF-21 was shown to be expressed and secreted from murine muscle cells in response to insulin stimulation...
  56. ncbi Antibodies to CBir1 flagellin define a unique response that is associated independently with complicated Crohn's disease
    Stephan R Targan
    Cedars Sinai Inflammatory Bowel Disease Center, Los Angeles, California 90048, USA
    Gastroenterology 128:2020-8. 2005
    ....
  57. doi Autoimmunity against myelin oligodendrocyte glycoprotein is dispensable for the initiation although essential for the progression of chronic encephalomyelitis in common marmosets
    S Anwar Jagessar
    Department of Immunobiology, Biomedical Primate Research Centre, Rijswijk, The Netherlands
    J Neuropathol Exp Neurol 67:326-40. 2008
    ....
  58. doi Subversion of complement activation at the bacterial surface promotes serum resistance and opsonophagocytosis of Francisella tularensis
    Abdelhakim Ben Nasr
    Department of Microbiology and Immunology, Medical Research Building, University of Texas Medical Branch, Galveston, TX, USA
    J Leukoc Biol 84:77-85. 2008
    ..C3b inactivation also leads to inefficient MAC assembly, which contributes to the ability of this bacterium to resist complement lysis...
  59. pmc Amyloid precursor protein regulates brain apolipoprotein E and cholesterol metabolism through lipoprotein receptor LRP1
    Qiang Liu
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Neuron 56:66-78. 2007
    ....
  60. ncbi Prevalence of antibodies against Mycobacterium avium subsp paratuberculosis among beef cow-calf herds
    D A Dargatz
    USDA Animal and Plant Health Inspection Service, Centers for Epidemiology and Animal Health, Ft Collins, CO 80521, USA
    J Am Vet Med Assoc 219:497-501. 2001
    ..CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that the prevalence of antibodies to M avium subsp paratuberculosis among beef cows in the United States is low. Herds with seropositive animals were widely distributed geographically...
  61. ncbi Evaluation of protective efficacy and immune mechanisms of using a non-structural protein NS1 in DNA vaccine against dengue 2 virus in mice
    Shu Fen Wu
    Department of Microbiology and Immunology, National Defense Medical Center, P O Box 90048 505, Neihu, Taipei, Taiwan, ROC
    Vaccine 21:3919-29. 2003
    ..In summary, our results suggest the potential of NS1-DNA vaccine against DEN infection, and indicate both NS1-specific humoral and cellular immune responses contribute to the protection...
  62. ncbi Implication of cartilage intermediate layer protein in cartilage destruction in subsets of patients with osteoarthritis and rheumatoid arthritis
    J Tsuruha
    St Marianna University School of Medicine, Kawasaki, Japan
    Arthritis Rheum 44:838-45. 2001
    ....
  63. ncbi Detection and subtyping (H5 and H7) of avian type A influenza virus by reverse transcription-PCR and PCR-ELISA
    M Munch
    Danish Veterinary Laboratory, Arhus, Denmark
    Arch Virol 146:87-97. 2001
    ..As far as we know this is the first demonstration of RT-PCR detection on a panel of H7 strains using only one primer set...
  64. ncbi gammadelta-T cells expressing NK receptors predominate over NK cells and conventional T cells in the innate IFN-gamma response to Plasmodium falciparum malaria
    Diana S Hansen
    Infection and Immunity Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Vic, Australia
    Eur J Immunol 37:1864-73. 2007
    ..Applied to longitudinal cohort studies in endemic regions, similar comparative phenotyping should allow assessment of the contribution of diverse cell populations and regulatory receptors to risk of infection and disease...
  65. ncbi Production and characterization of mouse monoclonal antibodies reactive to Chikungunya envelope E2 glycoprotein
    Anne Claire Brehin
    Interactions Moléculaires Flavivirus Hôtes, Institut Pasteur, 75724 Paris, France
    Virology 371:185-95. 2008
    ..Anti-CHIK E2 MAbs 3C3, 3E4, and 8A4 should be helpful for studying the biology of CHIK virus and pathogenesis of disease. The combination of 8A4 and 3E4 is suitable for developing a specific antigen-capture ELISA...
  66. ncbi Evaluation of an IgG enzyme-linked immunosorbent assay for dengue diagnosis
    M P Miagostovich
    Department of Virology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil
    J Clin Virol 14:183-9. 1999
    ..The spread of dengue throughout the world and the increasing number of cases to be tested makes an ELISA-format test for IgG antibodies to replace the HI test highly desirable...
  67. pmc Serum TIMP-1, TIMP-2, and MMP-1 in patients with systemic sclerosis, primary Raynaud's phenomenon, and in normal controls
    S A Young-Min
    Department of Rheumatology, University of Newcastle upon Tyne, UK
    Ann Rheum Dis 60:846-51. 2001
    ..It is suggested that decreased serum levels of matrix metalloproteinases (MMPs) or increased levels of tissue inhibitors of matrix metalloproteinases (TIMPs) may account for this matrix accumulation...
  68. pmc Mice lacking the type I interferon receptor are resistant to Listeria monocytogenes
    Victoria Auerbuch
    Department of Molecular and Cell Biology, 508 Barker Hall, University of California, Berkeley, 94720, USA
    J Exp Med 200:527-33. 2004
    ..The results of this study suggest that L. monocytogenes might be exploiting an innate antiviral response to promote its pathogenesis...
  69. ncbi Aqueous humor levels of cytokines are related to vitreous levels and progression of diabetic retinopathy in diabetic patients
    Hideharu Funatsu
    Department of Ophthalmology, Diabetes Center, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Graefes Arch Clin Exp Ophthalmol 243:3-8. 2005
    ..It is unclear whether aqueous humor levels of vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) are related to the vitreous fluid levels of these substances and to the progression of diabetic retinopathy...
  70. ncbi Chlamydia trachomatis infection & female infertility
    Abida Malik
    Department of Microbiology, Aligarh Muslim University, Aligarh, India
    Indian J Med Res 123:770-5. 2006
    ..trachomatis is increasingly being associated with long-term complications like infertility. The present study was undertaken to assess the role of C. trachomatis in female infertility as such data are lacking...
  71. ncbi Regulation of endothelial matrix metalloproteinase-2 by hypoxia/reoxygenation
    Yaara Ben-Yosef
    Neuroimmunology Unit, Carmel Medical Center, Haifa, Israel
    Circ Res 90:784-91. 2002
    ..Prolonged hypoxia of ECs appears to enhance MMP-2 production and secretion, whereas reoxygenation further increases its level. These H/R-mediated effects on MMPs have the potential of enabling EC migration and possible angiogenesis...
  72. ncbi Hyperendemic human and porcine Taenia solium infection in Perú
    Hector H Garcia
    Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru
    Am J Trop Med Hyg 68:268-75. 2003
    ..Besides confirming the close relationship between taeniasis and cysticercosis cases, this large-scale field study demonstrated early age of tapeworm and cysticercosis infections in humans, and short duration of taeniasis infections...
  73. ncbi Detailed analysis of an experimental challenge model for Leishmania infantum (JPC strain) in dogs
    Jacqueline Poot
    Intervet International B V, Parasitology R and D, Wim de Korverstraat 35, 5831 AN Boxmeer, The Netherlands
    Vet Parasitol 130:41-53. 2005
    ..However, this procedure did result in infection in 80% of the dogs and is appealing for future development as a natural challenge model in vaccine development...
  74. pmc Toxocara seroprevalence in schizophrenic patients in Turkey
    Mustafa Kaplan
    Department of Medical Parasitology, Firat University, Faculty of Medicine, TR 23119 Elazig, Turkey
    Yonsei Med J 49:224-9. 2008
    ..To investigate the seroprevalence of toxocariasis in patients diagnosed as schizophrenia...
  75. ncbi Seroprevalence of Chikungunya virus infection on Grande Comore Island, union of the Comoros, 2005
    Kibet Sergon
    Epidemiology and Laboratory Training Program, Nairobi, Kenya Ministry of Health, Moroni, Comoros
    Am J Trop Med Hyg 76:1189-93. 2007
    ..The findings suggest that CHIKV was broadly transmitted during the outbreak with a high attack rate. Although not fatal during this outbreak, CHIKV infection caused significant morbidity and decreased economic productivity...
  76. pmc Application of phage display to high throughput antibody generation and characterization
    Darren J Schofield
    Abcam Ltd, Cambridge Science Park, Cambridge CB4 0FW, UK
    Genome Biol 8:R254. 2007
    ..Thus, we have demonstrated the potential of and illuminated the issues associated with genome-wide monoclonal antibody generation...
  77. pmc Detection of anti-arboviral immunoglobulin G by using a monoclonal antibody-based capture enzyme-linked immunosorbent assay
    A J Johnson
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80522, USA
    J Clin Microbiol 38:1827-31. 2000
    ..The tests were standardized for laboratory diagnosis of arboviral infections, with the intent that they be used in tandem with the corresponding IgM antibody-capture ELISAs...
  78. pmc Cerebrovascular transforming growth factor-beta contributes to inflammation in the Alzheimer's disease brain
    Paula Grammas
    Department of Pathology and the Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Am J Pathol 160:1583-7. 2002
    ..Our results suggest that vessel-derived TGF-beta contributes to inflammatory processes in the Alzheimer brain...
  79. doi Impact of the IL-4 -590 C/T transition on the levels of Plasmodium falciparum specific IgE, IgG, IgG subclasses and total IgE in two sympatric ethnic groups living in Mali
    Manijeh Vafa
    Department of Immunology, Stockholm University, S 106 91 Stockholm, Sweden
    Microbes Infect 11:779-84. 2009
    ..Taken together, these data indicate that the impact of IL-4 -590 variants on antibody levels may vary in different ethnic populations, and that this might affect the Ig-class and subclass distributions...
  80. ncbi A novel mechanism of action for anti-thymocyte globulin: induction of CD4+CD25+Foxp3+ regulatory T cells
    Marta Lopez
    Brigham and Women s Hospital, Transplantation Research Center, EBRC, 221 Longwood Avenue, 3rd Floor, Boston, MA 02115, USA
    J Am Soc Nephrol 17:2844-53. 2006
    ..These novel data suggest that ATG not only may promote expansion/generation of regulatory T cells but also may be useful in future ex vivo expansion of these cells for cellular therapy in autoimmunity and clinical transplantation...
  81. ncbi Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection
    V Kumarasamy
    Makmal Kesihatan Awam Kebangsaan National Public Health Laboratory, Kementerian Kesihatan, Lot 1853 Kg Melayu, 47000 Sungai Buloh, Selangor, Malaysia
    J Virol Methods 140:75-9. 2007
    ..The results indicate that the commercial dengue NS1 antigen-capture ELISA may be superior to virus isolation and RT-PCR for the laboratory diagnosis of acute dengue infection based on a single serum sample...
  82. ncbi Correlation of serum VEGF levels with clinical stage, therapy efficacy, tumor metastasis and patient survival in ovarian cancer
    Li Li
    Department of Gynecologic Oncology, Guangxi Medical University Cancer Institute and Hospital, Nanning 530021, China
    Anticancer Res 24:1973-9. 2004
    ....
  83. pmc West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H
    Kyung Min Chung
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 103:19111-6. 2006
    ..Accordingly, extracellular NS1 may function to minimize immune system targeting of West Nile virus by decreasing complement recognition of infected cells...
  84. pmc Commercial serological antibody detection tests for the diagnosis of pulmonary tuberculosis: a systematic review
    Karen R Steingart
    Division of Pulmonary and Critical Care Medicine, San Francisco General Hospital, University of California, San Francisco, California, United States of America
    PLoS Med 4:e202. 2007
    ..Currently, dozens of distinct commercial antibody detection tests are sold in developing countries. The question is "do they work?"..
  85. ncbi Influence of the dengue serotype, previous dengue infection, and plasma viral load on clinical presentation and outcome during a dengue-2 and dengue-4 co-epidemic
    Laurent Thomas
    Service des Urgences, Centre Hospitalier Universitaire, Fort de France, Martinique
    Am J Trop Med Hyg 78:990-8. 2008
    ..The most severe cases of dengue resulted from the combined effects of DENV-2 and secondary infection...
  86. ncbi Preclinical study of influenza virus A M2 peptide conjugate vaccines in mice, ferrets, and rhesus monkeys
    Jiang Fan
    Department of Vaccine and Biologics Research, Merck Research Laboratories, 770 Sumneytown Pike, P O Box 4, WP16 306, West Point, PA 19486, USA
    Vaccine 22:2993-3003. 2004
    ..The data presented here will permit better understanding of the potential of an M2-based vaccine approach...
  87. ncbi Corticotropin-releasing hormone and its structurally related urocortin are synthesized and secreted by human mast cells
    Duraisamy Kempuraj
    Department of Pharmacology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Endocrinology 145:43-8. 2004
    ..These findings indicate that mast cells are not only the target, but also a potential source of CRH and Ucn that could have both autocrine and paracrine functions, especially in allergic inflammatory disorders exacerbated by stress...
  88. ncbi Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents
    Chris Druar
    Cangene Corporation, Mississauga, ON, Canada
    FEMS Immunol Med Microbiol 52:78-87. 2008
    ..However, these ELISAs had low diagnostic accuracy in endemic regions, possibly due to previous patient exposure to B. pseudomallei...
  89. ncbi Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA
    J C ter Steege
    Department of Pediatrics, Academic Hospital Maastricht, The Netherlands
    Free Radic Biol Med 25:953-63. 1998
    ..0 +/- 28.2 microM in celiac disease patients. Both nitrotyrosine and NOx levels declined when the patients were on a gluten-free diet, suggesting a relation between intestinal inflammation and plasma nitrotyrosine and NOx levels...
  90. ncbi Sensitive ELISA detection of amyloid-beta protofibrils in biological samples
    Hillevi Englund
    Department of Public Health Molecular Geriatrics, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    J Neurochem 103:334-45. 2007
    ..This assay provides a novel tool for investigating the role of Abeta protofibrils in AD and has the potential of becoming an important diagnostic assay...
  91. ncbi Therapeutically effective antibodies against amyloid-beta peptide target amyloid-beta residues 4-10 and inhibit cytotoxicity and fibrillogenesis
    J McLaurin
    Centre for Research in Neurodegenerative Diseases, University of Toronto, Toronto, Ontario, Canada
    Nat Med 8:1263-9. 2002
    ..These findings provide the basis for improved immunization antigens as well as attempts to design small-molecule mimics as alternative therapies...
  92. ncbi Real-time PCR to improve the diagnosis of respiratory syncytial virus infection
    R Mentel
    Friedrich Loeffler Institute of Medical Microbiology, University Hospital, Ernst Moritz Arndt University Greifswald, Germany
    J Med Microbiol 52:893-6. 2003
    ..7 %) by nested RT-PCR. The assay was specific for RSV. The procedure offers a rapid and sensitive alternative to conventional RT-PCR. Closed-tube detection eliminates the risk of contamination...
  93. ncbi Dengue virus infection and immune response in humanized RAG2(-/-)gamma(c)(-/-) (RAG-hu) mice
    Jes G Kuruvilla
    Department of Microbiology, Immunology and Pathology, 1619 Campus Delivery, Colorado State University, Fort Collins, CO 80523 1619, USA
    Virology 369:143-52. 2007
    ..These results demonstrate for the first time that humanized mice are capable of dengue viral primary human immune responses thus paving the way for new dengue immunopathogenesis and vaccine studies...
  94. ncbi A Toll-like receptor-independent antiviral response induced by double-stranded B-form DNA
    Ken J Ishii
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Nat Immunol 7:40-8. 2006
    ..These results suggest that both TBK1 and IKKi are required for innate immune activation by B-DNA, which might be important in antiviral innate immunity and other DNA-associated immune disorders...
  95. pmc Serum neutrophil gelatinase-associated lipocalin (NGAL) as a marker of acute kidney injury in critically ill children with septic shock
    Derek S Wheeler
    Division of Critical Care Medicine, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
    Crit Care Med 36:1297-303. 2008
    ..To validate serum neutrophil gelatinase-associated lipocalin (NGAL) as an early biomarker for acute kidney injury in critically ill children with septic shock...
  96. pmc Broad neutralization of human immunodeficiency virus type 1 (HIV-1) elicited from human rhinoviruses that display the HIV-1 gp41 ELDKWA epitope
    Gail Ferstandig Arnold
    Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854 5638, USA
    J Virol 83:5087-100. 2009
    ....
  97. ncbi Epigallocatechin gallate modulates cytokine production by bone marrow-derived dendritic cells stimulated with lipopolysaccharide or muramyldipeptide, or infected with Legionella pneumophila
    James Rogers
    Department of Medical Microbiology and Immunology, University of South Florida, Tampa, FL 33612, USA
    Exp Biol Med (Maywood) 230:645-51. 2005
    ..Further studies are necessary to characterize the physiologic function of the effect of EGCG on TNF-alpha and IL-12 during Lp infection, and the mechanisms involved...
  98. ncbi NGAL is an early predictive biomarker of contrast-induced nephropathy in children
    Russel Hirsch
    Division of Cardiology, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH, USA
    Pediatr Nephrol 22:2089-95. 2007
    ..52, p < 0.0001) and plasma (R (2) = 0.72, p < 0.0001) were found to be powerful independent predictors of CIN. Patient demographics and contrast volume were not predictive of CIN...
  99. ncbi Plasma Abeta(1-40) and Abeta(1-42) and the risk of dementia: a prospective case-cohort study
    Marieke van Oijen
    Department of Epidemiology and Biostatistics, Erasmus Medical Centre, Rotterdam, The Netherlands
    Lancet Neurol 5:655-60. 2006
    ..However, Abeta(1-42) concentrations may decrease early in the dementia process. We postulated that concentrations of Abeta(1-40) and Abeta(1-42) in plasma are associated with risk of dementia...
  100. ncbi Early Dengue infection and outcome study (EDEN) - study design and preliminary findings
    Jenny G H Low
    Department of Infectious Diseases, Communicable Disease Centre, Tock Seng Hospital, Singapore
    Ann Acad Med Singapore 35:783-9. 2006
    ..Hence, the World Health Organization (WHO) guidelines on dengue diagnosis and management which were developed using the paediatric experiences, may not be suitable for the management of adult dengue infections...
  101. pmc Cyclic adenosine monophosphate is a key component of regulatory T cell-mediated suppression
    Tobias Bopp
    Institute for Immunology, Johannes Gutenberg University, 55131 Mainz, Germany
    J Exp Med 204:1303-10. 2007
    ..Hence, naturally occurring T reg cells unexpectedly may control the immune regulatory network by a well-known mechanism based on the intercellular transport of cAMP via gap junctions...

Research Grants62

  1. Hepcidin-based screening for infantile iron deficiency
    Mark D Fleming; Fiscal Year: 2010
    ....
  2. Regulation of Astrocyte TIMP-1 in HIV-Associated Dementia
    JEREL ADAM FIELDS; Fiscal Year: 2011
    ..analysis tools such as western blot, real-time polymerase chain reaction, immunocytochemistry and enzyme linked immunosorbent assay. Aim 2 will be completed by combining pathway-specific inhibitors with a panel of luciferase ..
  3. Effects of Cannabinoids on the Immune Response to Murine Systemic Candidiasis
    NANCY ELIZABETH BUCKLEY-NIEVES; Fiscal Year: 2012
    ..and splenic macrophages cytokine mRNA levels from cannabinoid and vehicle treated mice using enzyme linked immunosorbent assay and real time RT-PCR, respectively...
  4. Biomarker Profiles for Early Diagnosis of Sepsis in Neonates
    JAMES PRESTON MAPES; Fiscal Year: 2011
    ....
  5. Continuation of FERN Activities at the Connecticut Agricultural Experiment Statio
    LAURIE J PETERSON-WRIGHT; Fiscal Year: 2013
    ..The CAES Department of Analytical Chemistry has demonstrated the expertise, capability, and adaptability to fully meet and exceed these program needs. ..
  6. Neuroimmune modulation across the BBB: Regulation of ILS trafficking by TNF
    Weihong Pan; Fiscal Year: 2012
    ..Their interactions with the blood-brain barrier largely determine the final outcome of these blood-borne cytokines. ..
  7. Mechanisms of mitochondrial fission/fusion dysregulation during HIV-1-associated
    JEREL ADAM FIELDS; Fiscal Year: 2013
    ..These studies will provide valuable insight into neuronal mitochondria dynamics during HAND and other CNS disease, and possibly provide novel therapeutic targets. ..
  8. Identification of a Multi-Analyte Profile for Primary Hyperoxaluria
    James P Mapes; Fiscal Year: 2012
    ..abstract_text> ..
  9. Prevention of diabetes by pDNA encoded with IL-10
    Jagdish Singh; Fiscal Year: 2010
    ..The proposed study will contribute towards the development of a high efficiency and low toxicity non-viral gene delivery vehicle in order to deliver plasmid encoding IL-10 gene for prevention of type 1 diabetes. ..
  10. Biomarker Profiles for Prediction and Diagnosis of Post-Transplant Renal Injury
    JAMES PRESTON contact MAPES; Fiscal Year: 2010
    ..The goal will be to improve the long-term outcome of recipients of thoracic and abdominal organ transplants by developing novel biomarker patterns that clinicians can use to predict, diagnose and monitor transplant outcomes. ..
  11. Central Mechanisms of Orofacial Pain
    Jianren Mao; Fiscal Year: 2012
    ..The outcome of this study could lead to the development of new therapeutic tools to treat often intractable and debilitating clinical orofacial pain. ..
  12. IgM Fc receptor in CLL
    Hiromi Kubagawa; Fiscal Year: 2013
    ..Evidence supporting the central hypothesis will greatly improve the understanding of the pathogenesis of CLL and justify the future exploration of the effectiveness of targeting Fc[unreadable]R in CLL. ..
  13. Micromagnetic Aptamer PCR System for Ultrasensitive Multiplexed Protein Detection
    HYONGSOK TOM SOH; Fiscal Year: 2010
    ....
  14. Mycobacterium Tuberculosis Lipoarabinomannan In Vivo
    Libin Shi; Fiscal Year: 2013
    ..The definition of the terminal ends of LAM in vivo will impact the future when a sensitive and specific anti-LAM antibody can be generated based on this study to enhance LAM based existing assays for use in resource poor regions. ..
  15. Development and Testing of Novel Glanders Vaccine Candidates
    Paul J Brett; Fiscal Year: 2011
    ....
  16. Food and Drug Administration Research FERN Chemistry Cooperative Agreement: MN De
    MICHELLE DAWN CAMPBELL; Fiscal Year: 2013
    ..1 ..
  17. Phase II Trial of Tiopronin in Aneurysmal Subarachnoid Hemorrhage
    EDWARD SANDER CONNOLLY; Fiscal Year: 2011
    ..This would spark further study that could further explore neuroprotective pre-treatment of cerebral ischemia, and thus help neutralize a major source of morbidity and mortality in this disease process. ..
  18. Development of a Hand-Held Cancer Biomarker Monitor
    Guodong Liu; Fiscal Year: 2011
    ..If the project is successful, the developed device can also be applied for the noninvasive monitoring of other tumor biomarkers in biological fluids such as blood. ..
  19. Photonic Crystal Enhanced Fluorescence: Development of Sensors Structures and Det
    Richard C Zangar; Fiscal Year: 2013
    ..The overall goal is to develop a highly sensitive, multiplexed, rapid, and automated assay platform for fluorescent microarray ELISA that can perform biomarker analysis on a droplet of plasma. ..
  20. Mechanisms of Lymphatic Pump-Enhancement of Immune Function
    LISA MICHELLE HODGE; Fiscal Year: 2012
    ..In addition, this new information will expand basic understanding of the lymphatic system and its role in immunological function. ..
  21. Improved Diagnosis of Kaposi's Sarcoma Herpes Virus Infection
    Steven D Dong; Fiscal Year: 2013
    ..Achieving the goals in this project would generate a commercial HHV-8 diagnostic test which will identify infected patients and donors to reduce transmission risk and to make the blood supply safer. ..
  22. Validation of a Vascular Health Profile for Cardiovascular Disease
    Emile R Mohler; Fiscal Year: 2010
    ..with diabetes mellitus (DM) and compared flow cytometry results with those of a non cell specific Enzyme Linked ImmunoSorbent assay (ELISA)...
  23. Food Safety and Security Monitoring Project (U18), FERN
    Abul Anisuzzaman; Fiscal Year: 2013
    ..The project narrative provides detailed information to address the complementary needs of the FDA and shows our thorough commitment to the FERN Chemistry Program. ..
  24. Nicotine, Neurotrophins and Depression
    Yousef Tizabi; Fiscal Year: 2009
    ..The findings may also facilitate the development and evaluation of novel antidepressants and/or compounds useful in smoking cessation. ..
  25. Biomarker for liver ischemia/reperfusion-induced injury
    MONIKA OLI; Fiscal Year: 2006
    ..The overall goal of this proposal is to develop a prototype ELISA kit for the quantitative determination of ASS as novel diagnostic biomarker in biological fluids including serum or plasma. [unreadable] [unreadable] [unreadable]..
  26. Plasmacytoid Dendritic Cells and Their Role in Transplant Tolerance
    Audrey H Lau; Fiscal Year: 2013
    ..Hepatic pDC have the potential to induce Ag specific tolerance in transplant models. By utilizing antigen specific cellular therapy, we have the potential to transform the outcome and management of organ transplantation. ..
  27. Drug Screening with Nano-Porous Silicon Optical Biosensors
    LISA BONANNO; Fiscal Year: 2010
    ..Ultimately this would also benefit the patient by providing doctors with results at the specific time of treatment. ..
  28. Nanoparticle-Hydrogel Strips for Detection of Staphylococcal Enterotoxins
    Chuanmin Ruan; Fiscal Year: 2009
    ..The successful outcome of this research should provide direct economic benefits to food and diagnostics industries and the whole society in terms of biosafety. ..
  29. Ultra-sensitive method for HIV p24 antigen detection
    SIMON BYSTRYAK; Fiscal Year: 2012
    ..Successful completion of these studies will be beneficial for public health, and make available all of the technology needed for a substantial business opportunity to license the technology and manufacture commercial products. ..
  30. High Dimensional Cytometric Assay for Clinical Assessment of Vascular Health
    Todd R Johnson; Fiscal Year: 2013
    ..with diabetes mellitus and compared flow cytometry results with those of a non cell specific Enzyme Linked ImmunoSorbent assay (ELISA)...
  31. UROTHELIAL CELL ACTIVATION IN INTERSTITIAL CYSTITIS
    Monica Liebert; Fiscal Year: 1993
    ....
  32. Biosimulation of vocal fold surgical injury
    YEE KEY LI; Fiscal Year: 2013
    ....
  33. Prospective Study of Serum MIS and Gynecologic Cancer Risk
    Joanne F Dorgan; Fiscal Year: 2013
    ..The proposed study also could potentially identify new avenues for research on ovarian and endometrial cancer risk assessment and screening. ..
  34. Meso-plex miRNA and protein profiling for cancer diagnostics using chip-integrate
    Mark D Johnson; Fiscal Year: 2013
    ..Well-established miRNA and protein biomarkers exist for glioblastoma, thus keeping the proposed efforts entirely on technology validation. ..
  35. FDA FERN Chemistry Cooperative Agreement 2010
    STEVEN SOBEK; Fiscal Year: 2013
    ..2. The use of Liquid Chromatography\Mass Spectrometry (LC\MS) analysis. 3. The use of Inductively Coupled Plasma\Mass Spectrometry (ICP\MS) analysis. 4. The use of Enzyme Linked Immunosorbent Assay (ELISA) analysis.
  36. Food Safety and Security Monitoring Project (U18), FERN Chemical Laboratories
    Shane A Wyatt; Fiscal Year: 2013
    ..A further area of study will be the investigation of Solid Phase MicroExtraction (SPME) for the detection of polyaromatic hydrocarbons in marine tissue. ..
  37. OREXIN AND THE CONTROL OF SLEEP AND WAKEFULNESS
    ROBERT MC CARLEY; Fiscal Year: 2005
    ..recording of behavioral states, measurement of extracellular levels of orexin peptides using enzyme linked immunosorbent assay (ELISA), and a method combining extracellular single unit recording with microdialysis-delivered ..
  38. MYELIN BASIC PROTEIN PEPTIDES IN BODY FLUIDS
    JOHN WHITAKER; Fiscal Year: 1993
    ....
  39. IMMUNOCHEMICAL STUDIES ON CARCINOGENIC MYCOTOXIN
    FUN CHU; Fiscal Year: 1990
    ..In addition, immunohistochemical techniques for monitoring the aflatoxin, ochratoxin, and T-2 toxin in the tissues of mice and rats that have ingested these mycotoxins have been developed. (HI) ..
  40. QUANTIFICATION OF SKIN ACRYLATE ADDUCTS
    Leena Nylander French; Fiscal Year: 2000
    ..Through biological monitoring of workers and exposure assessment using these new methods, strategies for minimizing exposure and, thus, preventing adverse health effects can be developed and implemented. ..
  41. Modulating the fate of tau in the Hsp70 chaperone system.
    ANDREA DOOLEY THOMPSON; Fiscal Year: 2012
    ..By therapeutically targeting a chaperone factor involved in the folding and degradation of misfolded proteins we may be able to take advantage of the body's own intrinsic system to treat AD. ..
  42. Validation of Fumonisin and Microcystin Biomarkers
    Jia Sheng Wang; Fiscal Year: 2003
    ..R. China, for exploring cancer risks from exposures to fumonisins and microcystins. ..
  43. Glucosyltransferase as a Marker for Caries Activity
    William Bowen; Fiscal Year: 2005
    ..Our eventual goal (in a future application) is to determine caries risk well before the onset of lesions and to identify those who are caries active short of cavitation or even white spot formation. ..
  44. Molecular Epidemiology of Recurrent Aphthous Stomatitis: Role of Ghrelin
    Lorena Baccaglini; Fiscal Year: 2009
    ..In particular, we are interested in knowing if patients with canker sores have a deficit of ghrelin (a newly discovered hormone involved in growth and mucosal protection from injury). ..
  45. DEVELOPMENT OF IMMUNO-PCR NEURODIAGNOSTIC KITS
    Arezoo Campbell; Fiscal Year: 2003
    ....
  46. HUMAN BLOOD PLATELET GLYCOPROTEIN IB
    Alan Michelson; Fiscal Year: 1991
    ....
  47. Biomarkers for Alcohol and Alcoholic Liver Disease
    PH D NEILSEN; Fiscal Year: 2013
    ..Future research is anticipated using these reagents and tools to test the possible role of PEth in ethanol-induced organ damage. ..
  48. 'Lyme disease: A possible test for cure
    MARIO PHILIPP; Fiscal Year: 2003
    ..Cure of LD will be assessed objectively (by culture and PCR) both in rhesus monkeys (chronic LD) and in mice (acute LD). Correlation between LD cure and anti-C6 Ab titers will be evaluated. ..
  49. FLOW/SURFACE EFFECTS ON PLATELET GPIIB-IIIA
    Steven Slack; Fiscal Year: 1992
    ..By means of such carefully controlled studies, they hope to better characterize factors governing blood-foreign material interactions...
  50. BIOLOGICAL MONITORING FOR EXPOSURE TO COAL TAR
    Regina Santella; Fiscal Year: 1991
    ..diol expoxide-DNA adducts will be used to monitor adduct formation in lymphocytes by competitive enzyme linked immunosorbent assay (ELISA)...
  51. CHARACTERIZATION OF A GHRH-LIKE SUBSTANCE IN RAT TESTIS
    ORA PESCOVITZ; Fiscal Year: 1992
    ..In summary, the experiments proposed in the current grant application will result in detailed characterization of a novel neuropeptide which may be important in modulation of testicular function...
  52. Designed Inhibitors of Pin1 in Mitosis
    FELICIA ETZKORN; Fiscal Year: 2009
    ..The best ligands will be useful in collaborations designed to elucidate the structure, function and dynamic relationships within Pin1 and between Pin1 and its ligands. ..
  53. An Enzyme Linked Immunosorbent Assay (ELISA) for Monitoring Galactosemia
    Menashi Cohenford; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  54. Development of immune assays for HPV-32
    Michael Hagensee; Fiscal Year: 2006
    ..The understanding of immune response to oral HPV infection may help to predict HPV disease progression as well as elucidate therapeutic and preventive strategies for HPV-related disease. ..
  55. TREATMENT OF TRAUMATIC BRAIN INJURY WITH SIMVASTATIN
    Asim Mahmood; Fiscal Year: 2009
    ..abstract_text> ..
  56. ENZYME IMMUNOASSAYS FOR THE DIAGNOSIS OF BLASTOMYCOSIS
    GENE SCALARONE; Fiscal Year: 1993
    ..The ultimate aim of this project is to develop sensitive and specific methods to detect antibodies and antigens in humans and animals with blastomycosis. ..
  57. Novel Biomarkers for Stroke Injury
    MONIKA OLI; Fiscal Year: 2005
    ..In addition, both the SBDP150 and SBDP120 ELISA will be validated in CSF and serum in a rodent model of stroke injury (MCAO injury). (Months 4-6) ..
  58. PHASE 2 & 3 TRIALS OF HIGH-DOSE ORAL N-ACETYLCYSTEINE IN CYSTIC FIBROSIS
    Carol Conrad; Fiscal Year: 2007
    ..To this end, pharmaceutical-grade N-acetylcysteine will be given orally in high doses. [unreadable] [unreadable] [unreadable]..
  59. GENETIC EPIDEMIOLOGY OF LIPOPROTEIN/LIPID LEVELS
    M Kamboh; Fiscal Year: 2000
    ....
  60. IMMUNOTHERAPY DURING GRAM-NEGATIVE BACTERIAL SEPSIS
    David Dunn; Fiscal Year: 1992
    ....
  61. Angiogenic & Premeability Growth Factors in Preeclampsia
    Donna Johnson; Fiscal Year: 2002
    ..In other words PIGF gene expression will be reduced and VEGF gene expression will be unchanged or increased. ..
  62. REGULATION OF JUVENILE HORMONE SYNTHESIS
    Barbara Stay; Fiscal Year: 2003
    ....