immunoenzyme techniques

Summary

Summary: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.

Top Publications

  1. ncbi Quantitative detection of increasing HIV type 1 antibodies after seroconversion: a simple assay for detecting recent HIV infection and estimating incidence
    Bharat S Parekh
    Division of AIDS, STD, TB Laboratory Research, and Division of HIV AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    AIDS Res Hum Retroviruses 18:295-307. 2002
  2. pmc Performance characteristics of the immunoglobulin G-capture BED-enzyme immunoassay, an assay to detect recent human immunodeficiency virus type 1 seroconversion
    Trudy Dobbs
    HIV Immunology and Diagnostics Branch, Mailstop D12, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA
    J Clin Microbiol 42:2623-8. 2004
  3. ncbi Snail, Slug, and Smad-interacting protein 1 as novel parameters of disease aggressiveness in metastatic ovarian and breast carcinoma
    Sivan Elloul
    Department of Pharmacology and Experimental Therapeutics, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel
    Cancer 103:1631-43. 2005
  4. ncbi Determination of mean recency period for estimation of HIV type 1 Incidence with the BED-capture EIA in persons infected with diverse subtypes
    Bharat S Parekh
    Division of Global AIDS Program, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    AIDS Res Hum Retroviruses 27:265-73. 2011
  5. ncbi Gene expression profiling of breast cell lines identifies potential new basal markers
    E Charafe-Jauffret
    Marseille Cancer Institute, Laboratory of Molecular Oncology, Inserm Institut Paoli Calmettes, Marseille, France
    Oncogene 25:2273-84. 2006
  6. ncbi Dynamics of HIV viremia and antibody seroconversion in plasma donors: implications for diagnosis and staging of primary HIV infection
    Eberhard W Fiebig
    Department of Laboratory Medicine, University of California, San Francisco, CA, USA
    AIDS 17:1871-9. 2003
  7. ncbi Overexpression of hypoxia-inducible factor 1alpha is associated with an unfavorable prognosis in lymph node-positive breast cancer
    Monika Schindl
    Institute of Clinical Pathology, Department of Surgery, University of Vienna Medical School, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Clin Cancer Res 8:1831-7. 2002
  8. ncbi High syndecan-1 expression in breast carcinoma is related to an aggressive phenotype and to poorer prognosis
    Mattia Barbareschi
    Department of Pathology, Santa Chiara Hospital, Trento, Italy
    Cancer 98:474-83. 2003
  9. ncbi Basal-like breast cancer defined by five biomarkers has superior prognostic value than triple-negative phenotype
    Maggie C U Cheang
    Genetic Pathology Evaluation Centre, Vancouver Coastal Health Research Institute, British Columbia Cancer Agency, and University of British Columbia, Vancouver, British Columbia, Canada
    Clin Cancer Res 14:1368-76. 2008
  10. pmc Rab25 and CLIC3 collaborate to promote integrin recycling from late endosomes/lysosomes and drive cancer progression
    Marta A Dozynkiewicz
    Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, UK
    Dev Cell 22:131-45. 2012

Research Grants

  1. MoAbs for the Management of Pancreatic Cancer
    David Gold; Fiscal Year: 2005
  2. Uveal Melanoma Micrometastasis
    Pierre L Triozzi; Fiscal Year: 2010
  3. Molecular Regulation of TIMP-2 in Neuron Cell Cycle
    DIANE JAWORSKI; Fiscal Year: 2007
  4. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
  5. Cerebral Vasoregulation In Elderly with Stroke
    Vera Novak; Fiscal Year: 2007
  6. VEGF gene expression in central nervous system microvascular pericyte
    Paula Dore Duffy; Fiscal Year: 2007
  7. In utero dietary exposure to endocrine disruptors
    Leena Hilakivi Clarke; Fiscal Year: 2007
  8. Dynamical Assessment of Cerebral Autoregulation
    Vera Novak; Fiscal Year: 2007
  9. Vitamin D5 in Prevention of Mammary Carcinogenesis
    Rajendra Mehta; Fiscal Year: 2006
  10. PKCepsilon-Related Proteins in Lung Fibrosis
    Stanley Hoffman; Fiscal Year: 2006

Detail Information

Publications334 found, 100 shown here

  1. ncbi Quantitative detection of increasing HIV type 1 antibodies after seroconversion: a simple assay for detecting recent HIV infection and estimating incidence
    Bharat S Parekh
    Division of AIDS, STD, TB Laboratory Research, and Division of HIV AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    AIDS Res Hum Retroviruses 18:295-307. 2002
    ..This assay should be useful for detecting recent HIV-1 infection and for estimating incidence among diverse HIV-1 subtypes worldwide...
  2. pmc Performance characteristics of the immunoglobulin G-capture BED-enzyme immunoassay, an assay to detect recent human immunodeficiency virus type 1 seroconversion
    Trudy Dobbs
    HIV Immunology and Diagnostics Branch, Mailstop D12, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA
    J Clin Microbiol 42:2623-8. 2004
    ..This information provides baseline data for the successful transfer of BED-CEIA to other laboratories and the use of BED-CEIA for the detection of recent HIV seroconversion and the calculation of incidence estimates worldwide...
  3. ncbi Snail, Slug, and Smad-interacting protein 1 as novel parameters of disease aggressiveness in metastatic ovarian and breast carcinoma
    Sivan Elloul
    Department of Pharmacology and Experimental Therapeutics, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel
    Cancer 103:1631-43. 2005
    ..For the current study, the authors analyzed the relation between the expression of Snail, Slug, and Sip1; the expression of MMP-2 and E-cadherin; and clinical parameters in patients with metastatic ovarian and breast carcinoma...
  4. ncbi Determination of mean recency period for estimation of HIV type 1 Incidence with the BED-capture EIA in persons infected with diverse subtypes
    Bharat S Parekh
    Division of Global AIDS Program, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    AIDS Res Hum Retroviruses 27:265-73. 2011
    ..The determination of ω with more data and new methodology suggests that ω of the BED-CEIA varies between different subtypes and/or populations. These estimates for ω may affect incidence estimates in various studies...
  5. ncbi Gene expression profiling of breast cell lines identifies potential new basal markers
    E Charafe-Jauffret
    Marseille Cancer Institute, Laboratory of Molecular Oncology, Inserm Institut Paoli Calmettes, Marseille, France
    Oncogene 25:2273-84. 2006
    ....
  6. ncbi Dynamics of HIV viremia and antibody seroconversion in plasma donors: implications for diagnosis and staging of primary HIV infection
    Eberhard W Fiebig
    Department of Laboratory Medicine, University of California, San Francisco, CA, USA
    AIDS 17:1871-9. 2003
    ..The characterization of primary HIV infection by the analysis of serial plasma samples from newly infected persons using multiple standard viral assays...
  7. ncbi Overexpression of hypoxia-inducible factor 1alpha is associated with an unfavorable prognosis in lymph node-positive breast cancer
    Monika Schindl
    Institute of Clinical Pathology, Department of Surgery, University of Vienna Medical School, Wahringer Gurtel 18 20, A 1090 Vienna, Austria
    Clin Cancer Res 8:1831-7. 2002
    ..The aim of this study was to determine the prognostic influence of HIF-1alpha expression in patients with advanced-stage breast cancer, evident by positive lymph nodes...
  8. ncbi High syndecan-1 expression in breast carcinoma is related to an aggressive phenotype and to poorer prognosis
    Mattia Barbareschi
    Department of Pathology, Santa Chiara Hospital, Trento, Italy
    Cancer 98:474-83. 2003
    ..Syndecan-1 is a transmembrane heparan sulphate proteoglycan that is involved in cell-cell adhesion, organization of cell-matrix adhesion, and regulation of growth factor signaling...
  9. ncbi Basal-like breast cancer defined by five biomarkers has superior prognostic value than triple-negative phenotype
    Maggie C U Cheang
    Genetic Pathology Evaluation Centre, Vancouver Coastal Health Research Institute, British Columbia Cancer Agency, and University of British Columbia, Vancouver, British Columbia, Canada
    Clin Cancer Res 14:1368-76. 2008
    ..This study directly compares the prognostic significance between three- and five-biomarker surrogate panels to define intrinsic breast cancer subtypes, using a large clinically annotated series of breast tumors...
  10. pmc Rab25 and CLIC3 collaborate to promote integrin recycling from late endosomes/lysosomes and drive cancer progression
    Marta A Dozynkiewicz
    Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, UK
    Dev Cell 22:131-45. 2012
    ..The identification of CLIC3 as a regulator of a recycling pathway and as an independent prognostic indicator in PDAC highlights the importance of active integrin trafficking as a potential drive to cancer progression in vivo...
  11. ncbi Temsirolimus inhibits malignant pleural mesothelioma growth in vitro and in vivo: synergism with chemotherapy
    Mir Alireza Hoda
    Division of Thoracic Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria
    J Thorac Oncol 6:852-63. 2011
    ..Here, we investigated the feasibility of mammalian target of rapamycin (mTOR) inhibition by temsirolimus as an antimesothelioma strategy...
  12. ncbi Peptidylarginine deiminase modulates the physiological roles of enolase via citrullination: links between altered multifunction of enolase and neurodegenerative diseases
    Byungki Jang
    Ilsong Institute of Life Science, Hallym University, 1605 4 Gwanyang dong, Dongan Gu, Anyang, Gyeonggi do 432 060, Republic of Korea
    Biochem J 445:183-92. 2012
    ..These findings suggest that PAD-mediated citrullination regulates the diverse physiological activities of enolase and that CE may be a candidate diagnostic/prognostic factor for degenerative diseases...
  13. ncbi Epidermal growth factor receptor overexpression in malignant pleural mesothelioma: prognostic correlations
    Ottavio Rena
    Thoracic Surgery Unit, University of Eastern Piedmont, Azienda Ospedaliero Universitaria Maggiore della Carita, C so Mazzini, 18, Novara, Italy
    J Surg Oncol 104:701-5. 2011
    ..To evaluate epidermal growth factor receptor (EGFR) phenotypic expression and related gene status in malignant pleural mesothelioma (MPM) and to correlate the results with patients' prognosis...
  14. ncbi Expression of hedgehog pathway components is associated with bladder cancer progression and clinical outcome
    Hui Chan He
    Department of Urology, Guangzhou First Municipal People s Hospital, Guangzhou Medical College, Guangzhou, 510180, China
    Pathol Oncol Res 18:349-55. 2012
    ..Expression of Shh, Ptch1 and Gli1 proteins was greater in bladder cancers than in the adjacent normal tissues. The examination of their expression is potentially valuable in prognostic evaluation of bladder cancer...
  15. pmc Prognostic relevance of Bmi-1 expression and autoantibodies in esophageal squamous cell carcinoma
    Wan li Liu
    Department of Experimental Research, Sun Yat sen University Cancer Center, Guangzhou, China
    BMC Cancer 10:467. 2010
    ..The objective of this study was to determine the level of Bmi-1 expression or its autoantibodies in human esophageal squamous cell carcinoma (ESCC) and to correlate it with clinicopathologic data...
  16. pmc Prognostic value of HMGB1 overexpression in resectable gastric adenocarcinomas
    Guoqiang Bao
    Department of General Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi an 710038, China
    World J Surg Oncol 8:52. 2010
    ..In the present study, HMGB1 overexpression and its correlation with the clinicopathologic characteristics and recurrence-free survival were evaluated in gastric adenocarcinomas...
  17. pmc Detection of recent HIV-1 infection using a new limiting-antigen avidity assay: potential for HIV-1 incidence estimates and avidity maturation studies
    Yen T Duong
    Division of Global HIV AIDS, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
    PLoS ONE 7:e33328. 2012
    ..We previously described a single-well limiting-antigen avidity enzyme immunoassay (LAg-Avidity EIA) to detect recent HIV-1 infection...
  18. pmc The selective hypoxia inducible factor-1 inhibitor PX-478 provides in vivo radiosensitization through tumor stromal effects
    David L Schwartz
    Department of Radiation Oncology, The University of Texas M D Anderson Cancer Center, Unit 97, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Mol Cancer Ther 8:947-58. 2009
    ..Successful translation of targeted HIF-1 radiosensitization to the clinical setting will require specific consideration of tumor microenvironmental effects and mechanisms...
  19. ncbi Prevalence of primary versus reactivated Epstein-Barr virus infection in patients with VCA IgG-, VCA IgM- and EBNA-1-antibodies and suspected infectious mononucleosis
    Tone Wikene Nystad
    Department of Microbiology and Immunology, The Gade Institute, Haukeland University Hospital, N 5021 Bergen, Norway
    J Clin Virol 38:292-7. 2007
    ....
  20. ncbi Spatial and temporal circulation of dengue virus serotypes: a prospective study of primary school children in Kamphaeng Phet, Thailand
    Timothy P Endy
    Department of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    Am J Epidemiol 156:52-9. 2002
    ..The authors' findings emphasize the complexity of dengue serotype-specific virus transmission and severe dengue disease and have important implications for dengue control and vaccine development...
  21. ncbi Development of two avidity-based assays to detect recent HIV type 1 seroconversion using a multisubtype gp41 recombinant protein
    Xierong Wei
    Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    AIDS Res Hum Retroviruses 26:61-71. 2010
    ..The new avidity-based assays using rIDR-M antigen may improve the accuracy of detecting recent HIV-1 infection and allow a better estimation of incidence in diverse HIV-1 subtypes...
  22. ncbi Early recurrence of benign meningioma correlates with expression of mini-chromosome maintenance-2 protein
    D P J Hunt
    Department of Pathology, University of Cambridge, UK
    Br J Neurosurg 16:10-5. 2002
    ..In conclusion, these results suggest that analysis of Mcm2 expression may facilitate identification of patients with a high risk of meningioma recurrence, for whom adjuvant radiotherapy may be of benefit...
  23. ncbi Aberrant expression of SWI/SNF catalytic subunits BRG1/BRM is associated with tumor development and increased invasiveness in prostate cancers
    Aijing Sun
    Department of Pathology, Shaoxing People s Hospital, First Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang, China
    Prostate 67:203-13. 2007
    ..Brahma gene (BRM) and Brahma-related gene 1 (BRG1) are major components with ATPase enzymatic activities in the nucleosome remodeling SWI/SNF complex, and their expression pattern in human prostate cancers is unknown...
  24. ncbi Association of CXCR4 and CCR7 chemokine receptor expression and lymph node metastasis in human cervical cancer
    J Kodama
    Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan, and Inner Mongolia Agriculture University Hospital, Huhehaote, China
    Ann Oncol 18:70-6. 2007
    ..The chemokine receptors CXCR4 and CCR7 have been suggested to play an important role in cancer invasion and metastasis. The expression of these receptors in human cervical cancer, however, has seldom been characterized...
  25. pmc Relation of hypoxia inducible factor 1 alpha and 2 alpha in operable non-small cell lung cancer to angiogenic/molecular profile of tumours and survival
    A Giatromanolaki
    Department of Pathology, Democritus University of Thrace, P O Box 128, Alexandroupolis 68100, Greece
    Br J Cancer 85:881-90. 2001
    ..Targeting the HIF pathway may prove of importance in the treatment of NSCLC...
  26. ncbi CIP2A is overexpressed in non-small cell lung cancer and correlates with poor prognosis
    Qian Ze Dong
    Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China
    Ann Surg Oncol 18:857-65. 2011
    ..Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an oncoprotein inhibiting proteolytic degradation of c-MYC. In this study, we investigated the clinical relevance of CIP2A in NSCLC...
  27. ncbi Localization of D-beta-aspartic acid-containing proteins in human eyes
    Yuichi Kaji
    Department of Ophthalmology, University of Tsukuba, Institute of Clinical Medicine, Ibaraki, Japan
    Invest Ophthalmol Vis Sci 48:3923-7. 2007
    ..To reveal the role of the D-beta-Asp formation in the aging process of eyes, immunohistochemical localization of D-beta-Asp was investigated in ocular samples of various ages...
  28. ncbi New testing strategy to detect early HIV-1 infection for use in incidence estimates and for clinical and prevention purposes
    R S Janssen
    Division of HIV AIDS Prevention, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    JAMA 280:42-8. 1998
    ..Differentiating individuals with early human immunodeficiency virus 1 (HIV-1) infection from those infected for longer periods is difficult but important for estimating HIV incidence and for purposes of clinical care and prevention...
  29. ncbi Prognostic value of biomarkers in malignant melanoma
    M Hernberg
    Department of Internal Medicine, University of Helsinki, Finland
    Melanoma Res 8:283-91. 1998
    ..Our findings suggest that Ki-67 and bcl-2 may be useful for predicting the prognosis of melanoma patients. Mdm-2 is a new but promising marker in melanoma and deserves further evaluation...
  30. ncbi MMPs, IL-1, and TNF are regulated by IL-17 in periodontitis
    A Beklen
    Department of Medicine Invärtes Medicin, Helsinki University Central Hospital, Helsinki, Finland
    J Dent Res 86:347-51. 2007
    ..Gingival fibroblasts may play an important role in tissue destruction in periodontitis via cytokine-inducible MMP-1 and MMP-3 production, in which IL-17 plays a role as a key regulatory cytokine...
  31. ncbi Sensitive ELISA detection of amyloid-beta protofibrils in biological samples
    Hillevi Englund
    Department of Public Health Molecular Geriatrics, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    J Neurochem 103:334-45. 2007
    ..This assay provides a novel tool for investigating the role of Abeta protofibrils in AD and has the potential of becoming an important diagnostic assay...
  32. ncbi Dating of human bocavirus infection with protein-denaturing IgG-avidity assays-Secondary immune activations are ubiquitous in immunocompetent adults
    Lea Hedman
    Department of Virology, Haartman Institute, University of Helsinki, PO Box 21, FIN 00014 Helsinki, Finland
    J Clin Virol 48:44-8. 2010
    ..Human bocavirus (HBoV) is a widespread human parvovirus causing acute respiratory illness in young children. The HBoV primary infections are viremic and can be diagnosed serologically...
  33. ncbi Angiogenesis in pterygium: morphometric and immunohistochemical study
    Arie L Marcovich
    Department of Ophthalmology, Kaplan Medical Center, Rehovot, Israel
    Curr Eye Res 25:17-22. 2002
    ..To evaluate the role of angiogenesis in the pathogenesis of pterygium by comparing the expression of von-Willebrand factor (vWF) and vascular endothelial growth factor (VEGF) in pterygium, and in normal superior bulbar conjunctiva...
  34. ncbi Clinical significance of BMP7 in human colorectal cancer
    Kazuo Motoyama
    Department of Molecular and Surgical Oncology, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumibaru, Beppu, 874 0838, Japan
    Ann Surg Oncol 15:1530-7. 2008
    ....
  35. pmc Co-expression of nuclear and cytoplasmic HMGB1 is inversely associated with infiltration of CD45RO+ T cells and prognosis in patients with stage IIIB colon cancer
    Rui Qing Peng
    State Key Laboratory of Oncology in South China, 651 Dongfeng R E, 510060 Guangzhou, China
    BMC Cancer 10:496. 2010
    ..This study examined whether high-mobility group box 1 (HMGB1), a damage-associated molecular pattern (DAMP) molecule, is involved in the infiltration of T cells and disease progression in locally advanced colon cancer...
  36. ncbi Low expression of claudin-4 is associated with poor prognosis in esophageal squamous cell carcinoma
    Chang Ohk Sung
    Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
    Ann Surg Oncol 18:273-81. 2011
    ..However, the prognostic significance of claudin-4 expression in esophageal squamous cell carcinoma (ESCC) remains to be clarified...
  37. ncbi Subtyping of undifferentiated non-small cell carcinomas in bronchial biopsy specimens
    Peh Sun Loo
    Department of Pathology, Aberdeen Royal Infirmary, Foresterhill, Aberdeen, Scotland, UK
    J Thorac Oncol 5:442-7. 2010
    ..Histochemical staining for mucin and immunohistochemical (IHC) identification of NSCLC subtype-associated markers could help predict the final subtype of resected NSCLCs diagnosed as NSCLC-NOS on preoperative bronchial biopsy samples...
  38. ncbi Decreased expression of updated NESG1 in nasopharyngeal carcinoma: its potential role and preliminarily functional mechanism
    Zhen Liu
    Cancer Research Institute, Southern Medical University, Guangdong Province, China
    Int J Cancer 128:2562-71. 2011
    ..Our findings first provided evidence that NESG1 may act as a tumor suppressor by inhibiting cell proliferation, invasion and migration of NPC cells...
  39. pmc First international consensus on the methodology of lymphangiogenesis quantification in solid human tumours
    I Van der Auwera
    Translational Cancer Research Group Antwerp, Laboratory of Pathology, University of Antwerp University Hospital Antwerp, Edegem 2650, Belgium
    Br J Cancer 95:1611-25. 2006
    ..With this consensus report, we therefore would like to initiate discussion on the standardisation of the immunohistochemical method for lymphangiogenesis assessment...
  40. ncbi Expression of epidermal growth factor receptor in esophageal and esophagogastric junction adenocarcinomas: association with poor outcome
    Kim L Wang
    Department of Pathology, The University of Texas M D Anderson Cancer Center, Houston, Texas, USA
    Cancer 109:658-67. 2007
    ..It is not known whether EGFR plays a similar role in esophageal and EGJ adenocarcinomas...
  41. pmc Lateral habenula projections to dopamine and GABA neurons in the rat ventral tegmental area
    Natalia Omelchenko
    Department of Neuroscience, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Eur J Neurosci 30:1239-50. 2009
    ..Moreover, the LHb may mediate a direct excitation of DA cells that is over-ridden by indirect inhibition originating from an extrinsic source...
  42. ncbi Use of laboratory tests and clinical symptoms for identification of primary HIV infection
    Frederick M Hecht
    Positive Health Program HIV Section at San Francisco General Hospital, tUniversity of California at San Francisco, 94110, USA
    AIDS 16:1119-29. 2002
    ..To determine the sensitivity and specificity of symptoms, three HIV-1 RNA assays, a p24 antigen EIA and a third-generation enzyme immunoassay (EIA) antibody test for diagnosis of primary HIV infection (PHI)...
  43. ncbi Transmission of occult hepatitis B virus by transfusion to adult and pediatric recipients in Taiwan
    Chun Jen Liu
    Division of Gastroenterology, Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, 1 Chang Te Street, Taipei 100, Taiwan
    J Hepatol 44:39-46. 2006
    ..To address this, we investigated the incidence of post-transfusion HBV infection after receiving screened blood units in Taiwan...
  44. ncbi Improved HIV-1 incidence estimates using the BED capture enzyme immunoassay
    John W Hargrove
    ZVITAMBO Project, Harare, Zimbabwe
    AIDS 22:511-8. 2008
    ..To validate the BED capture enzyme immunoassay for HIV-1 subtype C and to derive adjustments facilitating estimation of HIV-1 incidence from cross-sectional surveys...
  45. ncbi Vascular endothelial growth factor expression in stage I non-small cell lung cancer correlates with neoangiogenesis and a poor prognosis
    H Han
    Department of Pathology, University of Pittsburgh, Pennsylvania, USA
    Ann Surg Oncol 8:72-9. 2001
    ..Adjuvant therapies aimed at retarding tumor angiogenesis may be considered for stage I NSCLC patients with high VEGF levels...
  46. ncbi Effects of low HIV type 1 load and antiretroviral treatment on IgG-capture BED-enzyme immunoassay
    Tsunefusa Hayashida
    AIDS Clinical Center, International Medical Center of Japan, Tokyo, Japan
    AIDS Res Hum Retroviruses 24:495-8. 2008
    ..Those on antiretroviral treatment should be excluded from BED-CEIA testing to improve the predictive value of detecting recent infections...
  47. ncbi Evaluation of HER-2/neu gene amplification and overexpression: comparison of frequently used assay methods in a molecularly characterized cohort of breast cancer specimens
    Michael F Press
    Breast Cancer Research Program of the Lee Breast Center, Department of Pathology, University of Southern California, Los Angeles, USA
    J Clin Oncol 20:3095-105. 2002
    ..To compare and evaluate HER-2/neu clinical assay methods...
  48. pmc Cancer-associated fibroblasts are positively correlated with metastatic potential of human gastric cancers
    Kangkang Zhi
    Department of General Surgery, the Second Military Medical University affiliated Changhai hospital, Shanghai 200433, China
    J Exp Clin Cancer Res 29:66. 2010
    ..Thus raises the hypothesis that the extent of CAFs prevalence may help to establish the prognosis of gastric cancer patients...
  49. pmc Characterization of Treponema pallidum particle agglutination assay-negative sera following screening by treponemal total antibody enzyme immunoassays
    P A C Maple
    Health Protection Agency Birmingham, West Midlands Public Health Laboratory, Heart of England Foundation Trust, Bordesley Green East, Birmingham B9 5SS, United Kingdom
    Clin Vaccine Immunol 17:1718-22. 2010
    ..The reason why certain sera are negative by TPPA but reactive by treponemal EIA and other syphilis confirmatory assays is not clear, and these initial findings should be further explored...
  50. ncbi Increased expression of toll-like receptors (TLR) 2, 4 and 5 in gastric dysplasia
    Pedro Pimentel-Nunes
    Department of Physiology, University of Porto, Porto, Portugal
    Pathol Oncol Res 17:677-83. 2011
    ..Gastric dysplasia presents a high level of TLRs expression, suggesting that these receptors may play a role in adenocarcinoma development...
  51. ncbi Clinical recognition and diagnosis of Clostridium difficile infection
    John G Bartlett
    Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Clin Infect Dis 46:S12-8. 2008
    ..Often overlooked aspects of this diagnosis are high white blood cell counts (which are sometimes in the leukemoid range) and hypoalbuminemia...
  52. ncbi Evaluation of a sensitive/less sensitive testing algorithm using the bioMérieux Vironostika-LS assay for detecting recent HIV-1 subtype B' or E infection in Thailand
    Carrie L Young
    Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    AIDS Res Hum Retroviruses 19:481-6. 2003
    ....
  53. pmc Establishment and characterization of a new hypoxia-resistant cancer cell line, OCUM-12/Hypo, derived from a scirrhous gastric carcinoma
    Y Kato
    Department of Surgical Oncology, Osaka City University Graduate School of Medicine, 1 4 3 Asahi machi, Abeno Ku, Osaka, Japan
    Br J Cancer 102:898-907. 2010
    ..However, the mechanisms by which hypoxia affects the aggressive phenotypes remain unclear...
  54. ncbi Expression of TRAIL and TRAIL receptors in colon carcinoma: TRAIL-R1 is an independent prognostic parameter
    Jorn Strater
    Department of Pathology, University of Ulm, 89081 Ulm, Germany
    Clin Cancer Res 8:3734-40. 2002
    ..We examined the expression of TRAIL and its receptors in normal and neoplastic colon epithelium, and studied its correlation with prognosis in colon cancer...
  55. ncbi Significantly diminished long-term specificity of the BED capture enzyme immunoassay among patients with HIV-1 with very low CD4 counts and those on antiretroviral therapy
    Edmore T Marinda
    School of Public Health, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
    J Acquir Immune Defic Syndr 53:496-9. 2010
    ..To estimate the proportion who test as recent infections by the BED capture enzyme immunoassay (BED) among patients about to commence, and those receiving, antiretroviral therapy...
  56. ncbi Evaluation of HER2 protein expression in gastric carcinomas: comparative analysis of 1,414 cases of whole-tissue sections and 595 cases of tissue microarrays
    Kab Choong Kim
    Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea
    Ann Surg Oncol 18:2833-40. 2011
    ..Although the clinical relevance and prognostic significance of HER2 in breast cancer has been well acknowledged, it remains controversial in GC...
  57. pmc Polycystin-2 and phosphodiesterase 4C are components of a ciliary A-kinase anchoring protein complex that is disrupted in cystic kidney diseases
    Yun Hee Choi
    Department of Internal Medicine and Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 108:10679-84. 2011
    ....
  58. ncbi Reliability of tissue microarrays in detecting protein expression and gene amplification in breast cancer
    Daohai Zhang
    Molecular Diagnosis Centre, National University Hospital, National University of Singapore, Singapore
    Mod Pathol 16:79-84. 2003
    ....
  59. ncbi High expression of indoleamine 2,3-dioxygenase in the tumour is associated with medullary features and favourable outcome in basal-like breast carcinoma
    Jocelyne Jacquemier
    Département d Oncologie Moléculaire, Centre de Recherche en Cancerologie de Marseille, INSERM UMR89, IFR137, Marseille, France
    Int J Cancer 130:96-104. 2012
    ..0024). In conclusion, high IDO expression is associated with morphological medullary features and has an independent favourable prognostic value in BLBC...
  60. ncbi Plasma cell membrane localization of c-MET predicts longer survival in patients with malignant mesothelioma: a series of 157 cases from the MESOPATH Group
    Guénaëlle Levallet
    MESOPATH Center, Caen, France
    J Thorac Oncol 7:599-606. 2012
    ....
  61. pmc Routine laboratory screening for acute and recent HIV infection in Lima, Peru
    Jesse L Clark
    Department of Medicine, Division of Infectious Diseases and Program in Global Health, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Sex Transm Infect 86:545-7. 2010
    ..Before implementing screening programmes for acute HIV infection in developing countries, key issues, including cost, feasibility and public health impact, must be determined...
  62. pmc Mesothelin overexpression promotes mesothelioma cell invasion and MMP-9 secretion in an orthotopic mouse model and in epithelioid pleural mesothelioma patients
    Elliot L Servais
    Division of Thoracic Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Clin Cancer Res 18:2478-89. 2012
    ..The biologic function of MSLN overexpression in MPM is unknown. We hypothesized that MSLN may promote tumor invasion in MPM, a tumor characterized primarily by regional aggressiveness and rare distant metastases...
  63. ncbi European Society of Clinical Microbiology and Infectious Diseases (ESCMID): data review and recommendations for diagnosing Clostridium difficile-infection (CDI)
    M J T Crobach
    Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
    Clin Microbiol Infect 15:1053-66. 2009
    ..This approach resulted in non-significant improvements, and emphasizes the need for better diagnostic tests. Further studies to validate the applicability of two-step testing, including assessment of clinical features, are required...
  64. ncbi Prognostic value of hedgehog signaling pathway in patients with colon cancer
    Meihua Xu
    Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China
    Med Oncol 29:1010-6. 2012
    ..This is the first report describing about the relationship between hedgehog signaling pathway and the prognosis of colon cancer...
  65. ncbi Wnt5a signaling directly affects cell motility and invasion of metastatic melanoma
    Ashani T Weeraratna
    National Human Genome Research Institute, Cancer Genetics Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Cancer Cell 1:279-88. 2002
    ..Furthermore, Wnt5a expression in human melanoma biopsies directly correlated to increasing tumor grade. These observations support a role for Wnt5a in human melanoma progression...
  66. ncbi Immunohistochemical profiling based on Bcl-2, CD10 and MUM1 expression improves risk stratification in patients with primary nodal diffuse large B cell lymphoma
    J J F Muris
    Department of Pathology, VU Medical Centre, Amsterdam, The Netherlands
    J Pathol 208:714-23. 2006
    ..Its predictive power is stronger than previously published algorithms based on only GCB/ABC- or apoptosis-regulating proteins...
  67. ncbi Evaluation of enzyme immunoassay techniques for diagnosis of the most common intestinal protozoa in fecal samples
    Maha R Gaafar
    Parasitology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt
    Int J Infect Dis 15:e541-4. 2011
    ....
  68. ncbi Prognostic significance of CD151 expression in esophageal squamous cell carcinoma with aggressive cell proliferation and invasiveness
    Shigemasa Suzuki
    Department of General Surgical Science, Gunma University, Graduate School of Medicine, Maebashi, Gunma, Japan
    Ann Surg Oncol 18:888-93. 2011
    ..The purpose of this study was to clarify the clinicopathological outcome and prognostic significance of the immunohistochemical expression of CD151 in esophageal squamous cell carcinoma (ESCC)...
  69. ncbi BAG-1 enhances cell-cell adhesion, reduces proliferation and induces chaperone-independent suppression of hepatocyte growth factor-induced epidermal keratinocyte migration
    C A M Hinitt
    Oral and Dental Science, University of Bristol, Lower Maudlin Street, Bristol, BS1 2LY, UK
    Exp Cell Res 316:2042-60. 2010
    ..These findings are the first to suggest that BAG-1 plays a role in regulating cell-cell adhesion and suggest an important function in epidermal cohesion...
  70. ncbi Prognostic value of cyclin B1 in patients with esophageal squamous cell carcinoma
    Shinsuke Takeno
    Departments of Surgery II, Oita Medical University, Hasama machi, Oita, Japan
    Cancer 94:2874-81. 2002
    ..The objective of the current study was to clarify the role of the cell cycle regulators, cyclin B1 and p53, in patients with esophageal squamous cell carcinoma (ESCC)...
  71. ncbi Basal cell carcinoma of the prostate: unusual subtype of prostatic carcinoma
    Kazumasa Komura
    Department of Urology, Osaka Medical College, 2 7 Daigaku machi, Takatsuki, Osaka 569 8686, Japan
    Int J Clin Oncol 15:594-600. 2010
    ..Our findings reveal that tumors with a basaloid cell-predominant pattern have significant potential for a poor prognosis, in contrast with the conventional understanding regarding this neoplasm...
  72. pmc Global levels of histone modifications predict prognosis in different cancers
    David B Seligson
    Departments of Pathology and Laboratory Medicine, Human Genetics, David GeffenSchool of Medicine, University of California, Los Angeles, California 90095 1737, USA
    Am J Pathol 174:1619-28. 2009
    ....
  73. pmc Sec24b selectively sorts Vangl2 to regulate planar cell polarity during neural tube closure
    Janna Merte
    The Solomon H Snyder Department of Neuroscience and Howard Hughes Medical Institute, Johns Hopkins University, Baltimore, MD 21205, USA
    Nat Cell Biol 12:41-6; sup pp 1-8. 2010
    ..Thus, during COPII vesicle formation, Sec24b shows cargo specificity for a core PCP component, Vangl2, of which proper ER-to-Golgi transport is essential for the establishment of PCP, convergent extension and closure of the neural tube...
  74. ncbi HYAL1 overexpression is correlated with the malignant behavior of human breast cancer
    Jin xiang Tan
    Department of Endocrine Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China
    Int J Cancer 128:1303-15. 2011
    ..Further studies showed that the HYAL1, HYAL2 and HA were elevated in breast cancer, and HYAL1 could downregulate HA expression. In conclusion, HYAL1 may be a potential prognostic marker and therapeutic target in breast cancer...
  75. ncbi Prognostic value of ERCC1, thymidylate synthase, and glutathione S-transferase pi for 5-FU/oxaliplatin chemotherapy in advanced colorectal cancer
    Sung Hyun Kim
    Department of Internal Medicine, Dong A University College of Medicine, Busan, South Korea
    Am J Clin Oncol 32:38-43. 2009
    ....
  76. ncbi Mitogen-actived protein kinase activation is an early event in melanoma progression
    Cynthia Cohen
    Department of Pathology, Public Health, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Clin Cancer Res 8:3728-33. 2002
    ..Because of these findings, we wanted to assess the role of MAPK signaling in melanoma progression and angiogenesis...
  77. ncbi Overexpression of epidermal growth factor and hepatocyte growth factor receptors in a proportion of gastrinomas correlates with aggressive growth and lower curability
    Paolo L Peghini
    Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 8:2273-85. 2002
    ..Whether this is true of neuroendocrine tumors such as gastrinomas is unclear...
  78. ncbi Tumor suppressor function of Rab25 in triple-negative breast cancer
    Ji Ming Cheng
    Division of Hematology and Oncology, Department of Internal Medicine, Southern Illinois University School of Medicine and SimmonsCooper Cancer Institute at SIU, Springfield, IL 62794 9678, USA
    Int J Cancer 126:2799-812. 2010
    ..These findings suggest that Rab25 represents a novel class of cellular modulators that can influence both tumor initiation and the progression of the established tumors, thus ultimately affecting the biology of the malignant disease...
  79. ncbi Expression of CD133 correlates with differentiation of human colon cancer cells
    Hai liang Feng
    Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing, China
    Cancer Biol Ther 9:216-23. 2010
    ..Care must be taken if CD133 is used as the only marker of cancer stem cells in colon cancer, especially in established cell lines. CD133 negatively correlates with cell differentiation, but it is not a regulator of differentiation...
  80. ncbi Prevalence of IgG antibodies specific to Toxoplasma gondii among blood donors in Recife, Northeast Brazil
    Raquel A L Coêlho
    Laboratório de Imunopatologia Keizo Asami and Departamento de Bioquímica, Unviersidade Federal de Pernambuco, Recife, PE, Brasil
    Rev Inst Med Trop Sao Paulo 45:229-31. 2003
    ..4%). This percentage increases with age, ranging from 18.2% to 92.6% for individuals aging under 20 and 40-50 years old, respectively. For women of childbearing age (18-40 years) it was found a prevalence of 51.6%...
  81. pmc TLR9 expression in glioma tissues correlated to glioma progression and the prognosis of GBM patients
    Chao Wang
    Department of Neurosurgery, The First Affiliated Hospital of China Medical University, Shenyang, China
    BMC Cancer 10:415. 2010
    ....
  82. pmc Expression and co-expression of the members of the epidermal growth factor receptor (EGFR) family in invasive breast carcinoma
    D M Abd El-Rehim
    Department of Histopathology, The Breast Unit, Nottingham City Hospital NHS Trust and University of Nottingham, Hucknall Road, Nottingham NG5 1PB, UK
    Br J Cancer 91:1532-42. 2004
    ..These data imply that the combined profile expression patterns of the four receptor family members together provide more accurate information on the tumour behaviour than studying the expression of each receptor individually...
  83. ncbi Evaluation of seven oestrogen receptor beta antibodies for immunohistochemistry, western blotting, and flow cytometry in human breast tissue
    G P Skliris
    Molecular Medicine Unit, University of Leeds, Leeds, UK
    J Pathol 197:155-62. 2002
    ..However, there is considerable variation between the specificity and application of these antibodies, highlighting the fact that careful optimization is required when selecting an antibody for use in a particular laboratory technique...
  84. ncbi Histopathological and lymphangiogenic parameters in relation to lymph node metastasis in early stage oral squamous cell carcinoma
    Gary Warburton
    Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 4532, USA
    J Oral Maxillofac Surg 65:475-84. 2007
    ..In this study, we assess various clinical, histopathological and lymphangiogenic parameters in early stage oral SCC and their association with DNM...
  85. ncbi Disturbed synthesis of type II collagen interferes with rate of bone formation and growth and increases bone resorption in transgenic mice
    Jyrki Nieminen
    COXA Hospital for Joint Replacement, PO Box 652, FIN 33101 Tampere, Finland
    Calcif Tissue Int 82:229-37. 2008
    ..This led to a negative net bone balance and small bone size. The results support the idea that an altered synthesis of cartilage-specific molecule(s) can disturb postnatal bone development and growth...
  86. pmc S-100B concentrations predict disease-free survival in stage III melanoma patients
    S Kruijff
    Division of Surgical Oncology, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands
    Ann Surg Oncol 16:3455-62. 2009
    ..Elevation of the tumor marker S-100B in melanoma patients is a highly specific indicator of recurrence...
  87. ncbi Comparison of different enzyme-immunoassays for assessment of adrenocortical activity in primates based on fecal analysis
    M Heistermann
    Department of Reproductive Biology, German Primate Center, Gottingen, Germany
    Am J Primatol 68:257-73. 2006
    ..Nevertheless, regardless of which GC antibody is chosen, our study clearly reinforces the necessity of appropriately validating the respective assay system before it is used...
  88. ncbi Waning antibodies in measles and rubella vaccinees--a longitudinal study
    Jacques R Kremer
    Institute of Immunology and WHO Collaborative Centre for Measles and WHO European Regional Reference Laboratory for Measles and Rubella, Laboratoire National de Santé, 20A, rue Auguste Lumiere, L 1011, Luxembourg
    Vaccine 24:2594-601. 2006
    ..However, as a result of a more rapid decay of high-titre (> 1500 mIU) antibodies (-2.4%/year), many vaccinees may eventually become susceptible to vaccine-modified measles (VMM) and consequently complicate measles control strategies...
  89. pmc Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis
    Thérèse Rachell Theodoro
    Departamento de Bioquimica, Faculdade de Medicina do ABC, Sao Paulo, Brazil
    Neoplasia 9:504-10. 2007
    ..The results suggest that there is a tumor-inducing effect on heparanase expression by lymphocytes present in the PBMCs of breast cancer patients, which depends, in turn, on the interaction between a tumor and normal lymphocytes...
  90. ncbi Prognostic significance of vascular endothelial growth factor-C expression and lymphatic vessel density in supraglottic squamous cell carcinoma
    Seung Kuk Baek
    Department of Otolaryngology Head and Neck Surgery, Korea University College of Medicine, Seoul, South Korea
    Laryngoscope 119:1325-30. 2009
    ..The aim of this study is to identify the prognostic significance of vascular endothelial growth factor-C (VEGF-C) expression and lymphatic vessel density (LVD) in supraglottic cancer...
  91. ncbi Pulmonary sclerosing hemangioma consistently expresses thyroid transcription factor-1 (TTF-1): a new clue to its histogenesis
    A C Chan
    Department of Pathology, Queen Elizabeth Hospital, Hong Kong
    Am J Surg Pathol 24:1531-6. 2000
    ..These results suggest that pulmonary sclerosing hemangioma is an epithelial neoplasm derived from primitive respiratory epithelium or incompletely differentiated type II pneumocyte or Clara cell...
  92. ncbi Microwave decalcification of human temporal bones
    C D Cunningham
    Department of Otolaryngology--Head and Neck Surgery, Medical University of South Carolina, Charleston 29425, USA
    Laryngoscope 111:278-82. 2001
    ..Decalcification time is significantly reduced with no apparent adverse effects on structural preservation or antigenicity...
  93. pmc Augmented expression of secondary lymphoid tissue chemokine and EBI1 ligand chemokine in Crohn's disease
    D Kawashima
    Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1 4 3, Asahi machi, Abeno Ku, Osaka 545 8585, Japan
    J Clin Pathol 58:1057-63. 2005
    ..SLC/CCL21 and ELC/CCL19, chemokines that regulate T cell homing and promote recirculating T and dendritic cell (DC) interactions, help control antigen specific T cell responses...
  94. ncbi Beta-catenin accumulation and gene mutation in exon 3 in dedifferentiated liposarcoma and malignant fibrous histiocytoma
    Akio Sakamoto
    Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Arch Pathol Lab Med 126:1071-8. 2002
    ..beta-Catenin is an adhesion molecule that also plays a role in the Wnt signaling pathway. Objective.-To analyze beta-catenin mutation and accumulation in a series of liposarcomas and malignant fibrous histiocytomas...
  95. ncbi Fibroblast activation protein: purification, epitope mapping and induction by growth factors
    W J Rettig
    Program of Immunology, Memorial Sloan Kettering Cancer Center, New York, NY 10021
    Int J Cancer 58:385-92. 1994
    ..FAP and CD26 may belong to a family of structurally related but functionally distinct activation proteins that are expressed on different cell types and show unique modes of regulation in normal and malignant cells...
  96. ncbi Expression of Cdx2 and hepatocyte antigen in gastric carcinoma: correlation with histologic type and implications for prognosis
    Zhaoqing Fan
    Department of Surgery, Peking University School of Oncology and Beijing Cancer Hospital, Beijing, PR China
    Clin Cancer Res 11:6162-70. 2005
    ....
  97. ncbi Rapid human immunodeficiency virus-1 testing on labor and delivery in 17 US hospitals: the MIRIAD experience
    Denise J Jamieson
    Division of HIV AIDS Prevention, National Center for HIV AIDS, Viral Hepatitis, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Am J Obstet Gynecol 197:S72-82. 2007
    ..Routine rapid testing during labor provides a feasible, acceptable, and accurate way to identify HIV-infected women before delivery...
  98. ncbi Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung
    L Zhou
    Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, Ohio 45229 3039, USA
    J Histochem Cytochem 44:1183-93. 1996
    ..The early expression of TTF-1 and HNF-3beta, preceding and overlapping that of pro-SP-B, mature SP-B, pro-SP-C, and CCSP, supports a regulatory role for TTF-1 and HNF-3beta in lung-specific gene expression...
  99. pmc VEGF-A, VEGF-C, and VEGF-D in colorectal cancer progression
    M L George
    Colorectal Unit, Mayday University Hospital, Thornton Heath, Croydon, Surrey CR7 7YE, UK
    Neoplasia 3:420-7. 2001
    ..Increased expression of VEGF-A within CRCs was associated with lymphatic metastases, and therefore, this member of the VEGF family may be the most important in determining metastatic spread...
  100. ncbi Correlation of osteopontin protein expression and pathological stage across a wide variety of tumor histologies
    Domenico Coppola
    Department of Pathology, University of South Florida College of Medicine, Tampa, Florida, USA
    Clin Cancer Res 10:184-90. 2004
    ..Although various studies have assessed OPN protein levels in several tumor types, a broad survey of OPN expression in human neoplasia under the same experimental conditions has not been carried out...
  101. ncbi Lack of hypoxic response in uterine leiomyomas despite severe tissue hypoxia
    Arnulf Mayer
    Institute of Physiology and Pathophysiology, Johannes Gutenberg University, Mainz, Germany
    Cancer Res 68:4719-26. 2008
    ..Malignancy in the same organ system is associated with growth and metabolism beyond tissue-inherent limitations leading to the induction of hypoxia-related markers, thereby contributing to a self-perpetuating aggressive phenotype...

Research Grants79

  1. MoAbs for the Management of Pancreatic Cancer
    David Gold; Fiscal Year: 2005
    ..Finally, a PAM4IL2 fusion protein will be examined for its ability to enhance tumor uptake of a subsequently administered radiolabeled PAM4 antibody. ..
  2. Uveal Melanoma Micrometastasis
    Pierre L Triozzi; Fiscal Year: 2010
    ..Micrometastases, microscopic deposits of tumor that have spread via the bloodstream, are present in many patients with uveal melanoma at diagnosis. This project will test new methods of identifying and inhibiting these deposits. ..
  3. Molecular Regulation of TIMP-2 in Neuron Cell Cycle
    DIANE JAWORSKI; Fiscal Year: 2007
    ..abstract_text> ..
  4. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  5. Cerebral Vasoregulation In Elderly with Stroke
    Vera Novak; Fiscal Year: 2007
    ..The results of this study may improve our understanding of optimal BP targets for the maintenance of cerebral perfusion in elderly people with stroke. ..
  6. VEGF gene expression in central nervous system microvascular pericyte
    Paula Dore Duffy; Fiscal Year: 2007
    ..We propose that the CNS microvascular pericyte is a regulatory cell important in the vascular response to hypoxic stress and that cyclopentenone prostagladins are involved in the pericyte stress response. ..
  7. In utero dietary exposure to endocrine disruptors
    Leena Hilakivi Clarke; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  8. Dynamical Assessment of Cerebral Autoregulation
    Vera Novak; Fiscal Year: 2007
    ..Therefore, this project may have substantial socioeconomic impact. [unreadable] [unreadable] [unreadable]..
  9. Vitamin D5 in Prevention of Mammary Carcinogenesis
    Rajendra Mehta; Fiscal Year: 2006
    ..Whether prohibitin plays a role in cell transformation and chemopreventive efficacy in these cells? 3. How is 1alpha(OH)D5 metabolized in normal and neoplastic mammary epithelial cells and tissues? ..
  10. PKCepsilon-Related Proteins in Lung Fibrosis
    Stanley Hoffman; Fiscal Year: 2006
    ..These approaches will allow us to validate the role in lung fibrosis of the several target proteins that we have already identified. ..
  11. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  12. Roles of PTH/PTHrP Receptors in Osteoblastic Cells
    Laura Calvi; Fiscal Year: 2006
    ..abstract_text> ..
  13. HIV Vaccine Trials in Women
    Beryl Koblin; Fiscal Year: 2006
    ..The primary outcomes to be compared are measures of unprotected sex and vaccine knowledge scores. ..
  14. ABCA1 knock-in mouse model to study molecular pathology of Alzheimer's Disease
    Iliya Lefterov; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  15. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    ..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
  16. Endothelial Cell Death in Emphysema
    Rubin Tuder; Fiscal Year: 2007
    ..abstract_text> ..
  17. AAV-Based Plasmacytoid Dendritic Cell Cancer Vaccine
    Pierre L Triozzi; Fiscal Year: 2010
    ....
  18. Early experience and low 5-HT markers in alcohol abuse
    Mark Laudenslager; Fiscal Year: 2009
    ..Finally, involvement of the HPA axis in these relationships will be investigated as an exploratory goal. ..
  19. Eicosanoid Balance in Lung Transplant Injury and Repair
    David Pinsky; Fiscal Year: 2009
    ..This grant seeks to understand the balance between these injurious and protective substances, to develop strategies which may tip the balance in favor of protecting transplanted lungs from lethal injury. ..
  20. Efficacy of 25-hydroxyvitamin D3 in colon cancer chemoprevention
    Rajendra G Mehta; Fiscal Year: 2010
    ..The studies will provide a rationale for using 25(OH)D3 for the prevention of colon carcinogenesis. ..
  21. Timing of dietary exposure and breast cancer risk
    Leena Hilakivi Clarke; Fiscal Year: 2008
    ..This program aims to develop novel means to prevent some breast cancers by dietary modifications that take place during pregnancy and puberty. ..
  22. Molecular Classification and Therapy of CNS Lymphomas
    James Rubenstein; Fiscal Year: 2008
    ..We will perform gene expression profile analysis of these tumors from diagnostic specimens obtained from a frozen tumor bank to identify a set of genes, which can be used to predict outcome. ..
  23. THE VASCULAR BIOLOGY OF LUNG PRESERVATION
    David Pinsky; Fiscal Year: 2008
    ..These studies may lead to harnessing inspired "toxic" gases to improve outcomes after lung transplantation. [unreadable] [unreadable]..
  24. Iron Therapy in Renal Disease: Potential Toxicities
    RICHARD ZAGER; Fiscal Year: 2008
    ..Potential reasons for these differences will be assessed, with the ultimate goal of defining the safest way of administering parenteral irons to renal disease patients. ..
  25. NanoArrays in Mass Spectrometry for Proteomic and Metabolomic Applications
    Daojing Wang; Fiscal Year: 2008
    ..Towards this goal, we propose to utilize the enormous potential of nanotechnology to develop novel ionization substrates for mass spectrometry to study proteins and metabolites of single stem cells. [unreadable] [unreadable] [unreadable]..
  26. Mucin-degrading microflora for prophylactic antibiotics
    ROBERT CARMAN; Fiscal Year: 2008
    ..difficile, and reduces the incidence of C. difficile- induced antibiotic-associated diarrhea. [unreadable] [unreadable] [unreadable]..
  27. Cerebromicrovascular disease in elderly with diabetes
    Vera Novak; Fiscal Year: 2010
    ..This study will provide new data and biomarkers on cerebral perfusion and functional outcomes in type 2 DM, that can be used to initialize the efforts to implement brain protection as a new target for prevention of DM complications. ..
  28. Transcytosis of Flagellin Across Airway Epithelia
    ERIK LILLEHOJ; Fiscal Year: 2008
    ..Based on previous studies of ourselvesand others, we suggest that a major bacterial component (flagellin) is processed differently in CF lungs, leading to enhanced inflammatory responses. ..
  29. Changes in Functional Status Across Therapy for Primary Glioma
    Lee Jones; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  30. Characterization of the Chromosome 17q23 Amplicon
    Fergus Couch; Fiscal Year: 2006
    ..Thus, the project may involve a complete transition from benchtop to bedside. Finally, the amplified and overexpressed genes may prove useful as important targets of gene, pharmacological, and immunological therapy in the future. ..
  31. LEUKOCYTE ADHESION RECEPTORS AND THROMBOSIS IN STROKE
    David Pinsky; Fiscal Year: 2006
    ..Overall these studies will identify unifying molecular events which link micro vascular coagulation and inflammation in stroke. ..
  32. TRANSFORMING GROWTH FACTOR ALPHA AND LUNG INJURY
    WILLIAM HARDIE; Fiscal Year: 2003
    ..The experiments proposed will permit the P.I. to develop an independent line of research in a division dedicated to understanding the basic pathobiology of various lung diseases. ..
  33. BEHAVIORAL AND PHYSIOLOGICAL CONSEQUENCES OF LOSS
    Mark Laudenslager; Fiscal Year: 2003
    ..The present application seeks to identify developmental factors affecting the HPA axis and the balance of CORT and DHEA with consequences for individual differences in rates of progression in immunodeficiency disease. ..
  34. Methylphenidate & gene expression in the rat brain.
    JOAN BAIZER; Fiscal Year: 2004
    ..abstract_text> ..
  35. CNS MICROVASCULAR PERICYTE AND EAE
    Paula Dore Duffy; Fiscal Year: 2003
    ..The potential role of PC cytokines will be examined. ..
  36. ROBUST LINKAGE METHODS FOR HUMAN PEDIGREE DATA
    Katrina Goddard; Fiscal Year: 2004
    ..At the end of the project period, a comprehensive set of tools for model-free affected relative pair analysis will be available. ..
  37. Poly (ADP-Ribose) synthetase in expression of endothelia
    Basilia Zingarelli; Fiscal Year: 2003
    ..abstract_text> ..
  38. PRESERVATION AND CARDIAC TRANSPLANT VASCULOPATHY
    David Pinsky; Fiscal Year: 2003
    ..These experiments will provide new insights into mechanisms by which early ischemic injury leads to early graft failure and TCAD, and proffer new strategies for prevention. ..
  39. LEUKOREGULATION BY CD39 IN LUNG ISCHEMIA AND INFLAMMATI*
    David Pinsky; Fiscal Year: 2004
    ..abstract_text> ..
  40. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
  41. P21 INDUCTION BY BRCA2
    Fergus Couch; Fiscal Year: 2002
    ....
  42. R21 Project: Curcumin Treatment of Fibrosis
    Stanley Hoffman; Fiscal Year: 2002
    ..These experiments will demonstrate the efficacy and the scientific basis for that efficacy of a disease treatment already recommended by practitioners of alternative medicine. ..
  43. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  44. Osteoblastic Activation in the Hematopoietic Stem Cell
    Laura Calvi; Fiscal Year: 2005
    ..abstract_text> ..
  45. ACUTE RENAL FAILURE--IMPACT OF FLUORINATED ANESTHETICS
    RICHARD ZAGER; Fiscal Year: 2001
    ....
  46. Pregnancy Estrogens, Diet, and Breast Cancer Risk
    Leena Hilakivi Clarke; Fiscal Year: 2005
    ..abstract_text> ..
  47. VEGF PROTECTS AGAINST PULMONARY VASCULAR REMODELING
    Rubin Tuder; Fiscal Year: 2001
    ....
  48. MECHANISMS FOR CHEMOPREVENTION OF COLON CANCER
    CHINTHALAPALLY RAO; Fiscal Year: 2004
    ..Tissue distribution and comparative metabolism studies of curcumin and PEMC will be studied with synthetic [3H] curcumin and [3H]-PEMC in vivo in male F-344 rats. ..
  49. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
  50. THROMBOREGULATORY ROLE OF CD39 (ECTOADPASE) IN STROKE
    David Pinsky; Fiscal Year: 2004
    ..This collaboration, based on compelling feasibility data and historical collaborative success, will advance the understanding of CD39 thromboregulation, and lead to a novel therapeutic agent for thrombotic diatheses. ..
  51. CD44/VARIANT CYTOSKELETON IN BREAST CANCER PROGRESSION
    LILLY YW BOURGUIGNON; Fiscal Year: 2010
    ....
  52. C. difficile Toxin Membrane Test with Magnetic Particles
    ROBERT CARMAN; Fiscal Year: 2004
    ..perfringens enterotoxin, another cause of AAD. The technology developed in this project will be widely applicable for the development of new highly sensitive stool antigen tests. ..
  53. Potential for HIV transmission in relationships of drug-using women in Russia
    Linda Niccolai; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  54. NF KAPPA B MEDIATED CHEMORESISTANCE IN HUMAN LUNG CANCER
    David Jones; Fiscal Year: 2005
    ..The ultimate goal of this study is to provide the necessary background for the initiation of novel treatment strategies designed to treat patients with advanced lung cancer. ..
  55. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  56. DOPAMINE AND PLASTICITY IN RESPIRATORY CONTROL
    Rebecca Johnson; Fiscal Year: 2002
    ..End of Abstract) ..
  57. PATHOGENIC MECHANISMS OF VENOUS DISEASE
    Vincent Falanga; Fiscal Year: 2004
    ..The proposed studies should advance knowledge of the pathogenesis of venous ulceration and fibrosis. ..
  58. Therapeutic potential-LXR ligands in Alzheimer's Disease
    Iliya Lefterov; Fiscal Year: 2005
    ..abstract_text> ..
  59. Human Metapneumovirus Infection in Adults
    ANN FALSEY; Fiscal Year: 2007
    ..abstract_text> ..
  60. CD44-P185HER2 INTERACTION IN OVARIAN CANCER PROGRESSION
    LILLY BOURGUIGNON; Fiscal Year: 2007
    ..abstract_text> ..
  61. Tissue Engineering for the Treatment of Delayed Healing
    Vincent Falanga; Fiscal Year: 2007
    ..These studies will advance our understanding of how to reconstitute the wound bed and whether it is possible to correct the abnormal cellular phenotype in non-healing wounds. ..
  62. Social Modulation of Neurogenesis
    Lynn Almli; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  63. Yoga, Health, and Meditation
    Frederick Hecht; Fiscal Year: 2004
    ..abstract_text> ..
  64. Apoptosis as an anti-viral response in mosquito midgut
    ROLLIE CLEM; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  65. Middle Ear Epithelial Response to Cytokine Stimulation
    Joseph Kerschner; Fiscal Year: 2005
    ....
  66. Brainstem-Diencephalic Modulation of the Hippocampus
    ROBERT VERTES; Fiscal Year: 2006
    ..This work may have important implications for disorders of memory including Korsakoff's syndrome, diencephalic amnesia and Alzheimer's disease. ..
  67. SYNAPTIC ORGANIZATION OF THE BASOLATERAL AMYGDALA
    ALEXANDER MCDONALD; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  68. Renal Control of Blood Pressure in Early Diabetes
    Michael Brands; Fiscal Year: 2007
    ..whether increased SOD1 in transgenic mice protects against hypertension similar to tempol; d. the role of NADPH oxidase, by measuring p22 phox expression and the effect of apocynin. ..
  69. Tobacco Carcinogen Oral Cancer Model
    Joel Schwartz; Fiscal Year: 2005
    ..lc. Assessments of membrane (aryl hydrolase) and cytoplasmic phase I and II enzymes. ld. Confirmation of early cellular initiation and promotion events critical to our understanding for prevention of oral cancer. ..
  70. CIRCUITRY CONTROLLING THE HIPPOCAMPAL THETA RHYTHM
    ROBERT VERTES; Fiscal Year: 2002
    ..This work may have important implications for conditions such as Alzheimer's that involve a loss of cholinergic neurons of the MS/DBv. ..
  71. Interaction of COX-2 and nNOS in renin secretion
    Tianxin Yang; Fiscal Year: 2006
    ....
  72. STRUCTURE & FUNCTION RELATIONSHIP: PROSTANOID RECEPTORS
    Ke He Ruan; Fiscal Year: 2008
    ..Determine the solution conformation of segments comprising the intracellular domains. ..
  73. Prostaglandin I synthase, Thromboxane A synthase & Prostaglandin E synthase
    Ke He Ruan; Fiscal Year: 2009
    ....
  74. Generation of Collecting Duct Knockout of PPARgamma
    Tianxin Yang; Fiscal Year: 2006
    ..New information provided by this proposal is expected to provide insights into the roles of PPARgamma in regulation of fluid balance and in mediation of the side effects of TDZ. ..
  75. Allogeneic T Cell Responses Against Renal Cell Carcinoma
    Edus Warren; Fiscal Year: 2008
    ..3) Identify the genes encoding antigens recognized by RCC-reactive CD8+ T cell clones isolated from patients with metastatic RCC who exhibit tumor regression after nonmyeloablative HCT. ..
  76. Renal medullary COX-2 in blood pressure control
    Tianxin Yang; Fiscal Year: 2008
    ..The information resulted from these studies will provide new insight into the molecular mechanism responsible for long-term control of arterial blood pressure. ..
  77. Interaction of COX-2 AND nNOS in renin secretion (R01)
    Tianxin Yang; Fiscal Year: 2008
    ..abstract_text> ..