immunoblotting

Summary

Summary: Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.

Top Publications

  1. ncbi Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis
    Manuela Neumann
    Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 314:130-3. 2006
  2. ncbi How to interpret LC3 immunoblotting
    Noboru Mizushima
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo, Japan
    Autophagy 3:542-5. 2007
  3. pmc Promiscuous mutations activate the noncanonical NF-kappaB pathway in multiple myeloma
    Jonathan J Keats
    Comprehensive Cancer Center, Mayo Clinic Arizona, Scottsdale, AZ 85259, USA
    Cancer Cell 12:131-44. 2007
  4. pmc SIRT3 opposes reprogramming of cancer cell metabolism through HIF1α destabilization
    Lydia W S Finley
    Department of Pathology, The Paul F Glenn Labs for the Biological Mechanisms of Aging, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 19:416-28. 2011
  5. pmc The forkhead-associated domain protein Cep170 interacts with Polo-like kinase 1 and serves as a marker for mature centrioles
    Giulia Guarguaglini
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152, Martinsried, Germany
    Mol Biol Cell 16:1095-107. 2005
  6. ncbi Cytidine deamination of retroviral DNA by diverse APOBEC proteins
    Kate N Bishop
    Department of Infectious Diseases, Guy s, King s and St Thomas School of Medicine, King s College London, London, SE1 9RT, United Kingdom
    Curr Biol 14:1392-6. 2004
  7. pmc The physical relationship between infectivity and prion protein aggregates is strain-dependent
    Philippe Tixador
    INRA Institut National de la recherche Agronomique, UR892, Virologie Immunologie Moléculaires, Jouy en Josas, France
    PLoS Pathog 6:e1000859. 2010
  8. ncbi Induction of IRF-3/-7 kinase and NF-kappaB in response to double-stranded RNA and virus infection: common and unique pathways
    T Iwamura
    Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan
    Genes Cells 6:375-88. 2001
  9. ncbi Cdc42-MRCK and Rho-ROCK signalling cooperate in myosin phosphorylation and cell invasion
    Simon Wilkinson
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Nat Cell Biol 7:255-61. 2005
  10. ncbi Knockout of the alpha2 but not alpha1 5'-AMP-activated protein kinase isoform abolishes 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranosidebut not contraction-induced glucose uptake in skeletal muscle
    Sebastian B Jørgensen
    Copenhagen Muscle Research Centre, Department of Human Physiology, 13 Universitetsparken, University of Copenhagen, DK 2100 Copenhagen, Denmark
    J Biol Chem 279:1070-9. 2004

Research Grants

  1. Inhibition of human neutrophil function by Francisella tularensis
    Lee Ann H Allen; Fiscal Year: 2012
  2. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
  3. Axonopathy in Glaucoma
    Samuel D Crish; Fiscal Year: 2013
  4. Ferritin: Protein/mRNA/DNA in Fe/O Regulation/Metabolism
    ELIZABETH C contact THEIL; Fiscal Year: 2010
  5. The Role of Adipocyte-Induced Inflammation in Prostate Tumor Progression
    AIMALIE LYNNETTE HARDAWAY; Fiscal Year: 2012
  6. Iron, NO, and Lipid Peroxide in Photodynamic Therapy
    ALBERT GIROTTI; Fiscal Year: 2013
  7. Signal Transduction Mechanism of Osteoclast Differentiation
    Nandini Ghosh-Choudhury; Fiscal Year: 2013
  8. Role of G-gamma-7 in striatal cannabinoid and D2 dopamine
    Dana E Selley; Fiscal Year: 2010
  9. Dopamine Transporter Structure and Function
    Christopher K Surratt; Fiscal Year: 2010
  10. Epigenetic regulation of stem cell senescence in aging
    DAVID TOCHUKWU ASUZU; Fiscal Year: 2011

Detail Information

Publications435 found, 100 shown here

  1. ncbi Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis
    Manuela Neumann
    Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 314:130-3. 2006
    ..TDP-43 represents the common pathologic substrate linking these neurodegenerative disorders...
  2. ncbi How to interpret LC3 immunoblotting
    Noboru Mizushima
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo, Japan
    Autophagy 3:542-5. 2007
    ..However, LC3-II itself is degraded by autophagy, making interpretation of the results of LC3 immunoblotting problematic...
  3. pmc Promiscuous mutations activate the noncanonical NF-kappaB pathway in multiple myeloma
    Jonathan J Keats
    Comprehensive Cancer Center, Mayo Clinic Arizona, Scottsdale, AZ 85259, USA
    Cancer Cell 12:131-44. 2007
    ..These results highlight the critical importance of the NF-kappaB pathway in the pathogenesis of multiple myeloma...
  4. pmc SIRT3 opposes reprogramming of cancer cell metabolism through HIF1α destabilization
    Lydia W S Finley
    Department of Pathology, The Paul F Glenn Labs for the Biological Mechanisms of Aging, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 19:416-28. 2011
    ..Finally, we find that SIRT3 overexpression represses glycolysis and proliferation in breast cancer cells, providing a metabolic mechanism for tumor suppression...
  5. pmc The forkhead-associated domain protein Cep170 interacts with Polo-like kinase 1 and serves as a marker for mature centrioles
    Giulia Guarguaglini
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152, Martinsried, Germany
    Mol Biol Cell 16:1095-107. 2005
    ..We show that Cep170 labeling can be used to discriminate bona fide centriole overduplication from centriole amplification that results from aborted cell division...
  6. ncbi Cytidine deamination of retroviral DNA by diverse APOBEC proteins
    Kate N Bishop
    Department of Infectious Diseases, Guy s, King s and St Thomas School of Medicine, King s College London, London, SE1 9RT, United Kingdom
    Curr Biol 14:1392-6. 2004
    ..Overall, our findings show that although APOBEC family members are highly related, they exhibit significantly distinct antiviral characteristics that may provide new insights into host-pathogen interactions...
  7. pmc The physical relationship between infectivity and prion protein aggregates is strain-dependent
    Philippe Tixador
    INRA Institut National de la recherche Agronomique, UR892, Virologie Immunologie Moléculaires, Jouy en Josas, France
    PLoS Pathog 6:e1000859. 2010
    ..The distribution of PrP(Sc) and infectivity in the gradient was determined by immunoblotting and mouse bioassay, respectively...
  8. ncbi Induction of IRF-3/-7 kinase and NF-kappaB in response to double-stranded RNA and virus infection: common and unique pathways
    T Iwamura
    Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan
    Genes Cells 6:375-88. 2001
    ..The signal branches to a common NF-kappaB activation cascade, thus resulting in the activation of a set of critical transcription factors for the response...
  9. ncbi Cdc42-MRCK and Rho-ROCK signalling cooperate in myosin phosphorylation and cell invasion
    Simon Wilkinson
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Nat Cell Biol 7:255-61. 2005
    ..Our data show that a Cdc42-MRCK signal mediates myosin-dependent cell motility and highlight convergence between Rho and Cdc42 signalling...
  10. ncbi Knockout of the alpha2 but not alpha1 5'-AMP-activated protein kinase isoform abolishes 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranosidebut not contraction-induced glucose uptake in skeletal muscle
    Sebastian B Jørgensen
    Copenhagen Muscle Research Centre, Department of Human Physiology, 13 Universitetsparken, University of Copenhagen, DK 2100 Copenhagen, Denmark
    J Biol Chem 279:1070-9. 2004
    ..Alternatively, neither alpha-isoform of AMPK is involved in contraction-induced muscle glucose uptake...
  11. doi Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis
    Jesper V Olsen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried near Munich, Germany
    Sci Signal 3:ra3. 2010
    ..In particular, nuclear proteins and proteins involved in regulating metabolic processes have high phosphorylation site occupancy in mitosis. This suggests that these proteins may be inactivated by phosphorylation in mitotic cells...
  12. pmc Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association
    T Matsui
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 140:647-57. 1998
    ..b>Immunoblotting of serum-starved Swiss 3T3 cells with this mAb revealed that after LPA stimulation ERM proteins were rapidly ..
  13. pmc Toll-like receptor-mediated induction of type I interferon in plasmacytoid dendritic cells requires the rapamycin-sensitive PI(3)K-mTOR-p70S6K pathway
    Weiping Cao
    Emory Vaccine Center, Atlanta, GA 30329, USA
    Nat Immunol 9:1157-64. 2008
    ..Thus, mTOR signaling is crucial in TLR-mediated IFN-alpha/beta responses by pDCs...
  14. ncbi General nature of the STAT3-activated anti-inflammatory response
    Karim C El Kasmi
    Department of Infectious Diseases, St Jude s Children s Research Hospital, Memphis, TN 38105, USA
    J Immunol 177:7880-8. 2006
    ..We conclude that the AIR is a generic cytokine signaling pathway dependent on STAT3 but not unique to the IL-10R...
  15. ncbi GEP100 links epidermal growth factor receptor signalling to Arf6 activation to induce breast cancer invasion
    Masaki Morishige
    Department of Molecular Biology, Osaka Bioscience Institute, Osaka 565 0874, Japan
    Nat Cell Biol 10:85-92. 2008
    ..Our results indicate that GEP100 links EGFR signalling to Arf6 activation to induce invasive activities of some breast cancer cells, and hence may contribute to their metastasis and malignancy...
  16. pmc Linking of autophagy to ubiquitin-proteasome system is important for the regulation of endoplasmic reticulum stress and cell viability
    Wen Xing Ding
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15231, USA
    Am J Pathol 171:513-24. 2007
    ..Our studies thus provide a mechanistic view and elucidate the functional significance of the link between the two protein degradation systems...
  17. pmc Drp1 levels constitutively regulate mitochondrial dynamics and cell survival in cortical neurons
    Takuma Uo
    Department of Neurological Surgery, University of Washington School of Medicine, Seattle, 98195 6470, USA
    Exp Neurol 218:274-85. 2009
    ....
  18. ncbi Akt forms an intracellular complex with heat shock protein 90 (Hsp90) and Cdc37 and is destabilized by inhibitors of Hsp90 function
    Andrea D Basso
    Program in Pharmacology, Weill Graduate School of Medical Sciences, Cornell University and the Program in Cell Biology and Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 277:39858-66. 2002
    ..Thus, transduction of growth factor signaling via the Akt and Raf pathways requires functional Hsp90 and can be coordinately blocked by its inhibition...
  19. pmc Anti-inflammatory compounds parthenolide and Bay 11-7082 are direct inhibitors of the inflammasome
    Christine Juliana
    Department of Biochemistry and Molecular Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 285:9792-802. 2010
    ..Our results thus elucidate the molecular mechanism for the therapeutic anti-inflammatory activity of parthenolide and identify vinyl sulfones as a new class of potential therapeutics that target the NLRP3 inflammasome...
  20. pmc Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotype
    Yi Jiun Pan
    Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan
    J Clin Microbiol 46:2231-40. 2008
    ..We conclude that sequences from these two cps regions are very useful in detecting K57 and the new cps genotype...
  21. ncbi T helper 2 cytokines inhibit autophagic control of intracellular Mycobacterium tuberculosis
    James Harris
    Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, University of New Mexico School of Medicine, 915 Camino de Salud NE, Albuquerque, NM 87131, USA
    Immunity 27:505-17. 2007
    ..These findings establish a mechanism through which Th1-Th2 polarization differentially affects the immune control of intracellular pathogens...
  22. ncbi Sir2-dependent activation of acetyl-CoA synthetase by deacetylation of active lysine
    V J Starai
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706 1567, USA
    Science 298:2390-2. 2002
    ..b>Immunoblotting and mass spectrometry analysis showed that Salmonella enterica Acs enzyme activity is posttranslationally ..
  23. pmc Therapeutic trial of metformin and bortezomib in a mouse model of tuberous sclerosis complex (TSC)
    Neil Auricchio
    Translational Medicine Division, Brigham and Women s Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 7:e31900. 2012
    ..We conclude that neither bortezomib nor metformin has significant benefit in this native Tsc2(+/-) mouse model, which suggests limited benefit of these compounds in the treatment of TSC hamartomas and related lesions...
  24. pmc Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain
    Keisuke Tabata
    Department of Genetics, Graduate School of Medicine, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 21:4162-72. 2010
    ..Furthermore, we show that Rubicon but not PLEKHM1 uniquely regulates membrane trafficking via simultaneously binding both Rab7 and PI3-kinase...
  25. ncbi A CBP binding transcriptional repressor produced by the PS1/epsilon-cleavage of N-cadherin is inhibited by PS1 FAD mutations
    Philippe Marambaud
    Department of Psychiatry and Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Cell 114:635-45. 2003
    ..These data raise the possibility that FAD mutation-induced transcriptional abnormalities maybe causally related to the dementia associated with FAD...
  26. pmc Seipin is a discrete homooligomer
    Derk Binns
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, United States
    Biochemistry 49:10747-55. 2010
    ..Seipin appears as a toroid by negative staining electron microscopy. We speculate that seipin plays at least a structural role in organizing droplets or in communication between droplets and ER...
  27. pmc Delaying aging and the aging-associated decline in protein homeostasis by inhibition of tryptophan degradation
    Annemieke T van der Goot
    Department of Genetics, University of Groningen, University Medical Centre Groningen, 9700 RB, Groningen, The Netherlands
    Proc Natl Acad Sci U S A 109:14912-7. 2012
    ..With TDO and Indoleamine 2,3-dioxygenase as evolutionarily conserved human orthologs of TDO-2, intervening with tryptophan metabolism may offer avenues to reducing proteotoxicity in aging and age-related diseases...
  28. pmc Comparison of enzyme-linked immunosorbent assay, immunoblotting, and PCR for diagnosis of toxoplasmic chorioretinitis
    Odile Villard
    Institut de Parasitologie et Pathologie Tropicale, F 67000 Strasbourg, France
    J Clin Microbiol 41:3537-41. 2003
    ..diagnostic sensitivities of the three analytical techniques (enzyme-linked immunosorbent assay [ELISA], immunoblotting, and PCR) were compared by using a group of patients (n = 19) with suspected ocular toxoplasmosis...
  29. ncbi Integrin-specific activation of Rac controls progression through the G(1) phase of the cell cycle
    A Mettouchi
    Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 8:115-27. 2001
    ..This process is mediated by SOS and PI-3K and requires coordinate upstream signals through Shc and FAK. These results indicate that Rac is a crucial mediator of the integrin-specific control of cell cycle in endothelial cells...
  30. pmc Use of a Dictyostelium model for isolation of genetic loci associated with phagocytosis and virulence in Klebsiella pneumoniae
    Yi Jiun Pan
    Department of Microbiology, National Taiwan University College of Medicine, 1, Sec 1, Jen Ai Rd, Taipei 10051, Taiwan
    Infect Immun 79:997-1006. 2011
    ..Therefore, we have identified genes involved in resistance to phagocytosis in K. pneumoniae using Dictyostelium, and this model is useful to explore genes associated with resistance to phagocytosis in heavily encapsulated bacteria...
  31. pmc Casein kinase 1-dependent phosphorylation of familial advanced sleep phase syndrome-associated residues controls PERIOD 2 stability
    Naval P Shanware
    University of Wisconsin School of Medicine and Public Health, The Department of Pharmacology, Madison, WI 53705, USA
    J Biol Chem 286:12766-74. 2011
    ..Our combined findings provide new insights into PER2 regulation and the biochemical basis of FASPS...
  32. pmc Ligand-binding domain determines endoplasmic reticulum exit of AMPA receptors
    Sarah K Coleman
    Department of Biosciences, Division of Biochemistry, Viikki Biocenter, University of Helsinki, FI 00014 University of Helsinki, Finland
    J Biol Chem 285:36032-9. 2010
    ..Considered together with the essential role of bound agonist, our findings reveal the ligand-binding domain as the critical quality control target in AMPAR biogenesis...
  33. pmc Autoantibody profiles in the sera of European patients with myositis
    R Brouwer
    Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands
    Ann Rheum Dis 60:116-23. 2001
    ..Furthermore, our data show that some sera may contain more than one type of MSA and confirm the strong association of anti-Ro52 with anti-Jo-1 reactivity...
  34. pmc The adaptor function of TRAPPC2 in mammalian TRAPPs explains TRAPPC2-associated SEDT and TRAPPC9-associated congenital intellectual disability
    Min Zong
    School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, People s Republic of China
    PLoS ONE 6:e23350. 2011
    ..Here, we reveal that the mammalian equivalent of the TRAPPII complex is structurally distinct from the yeast counterpart thus leading to insight into mechanism of disease...
  35. pmc T cell antigen receptor signaling and immunological synapse stability require myosin IIA
    Tal Ilani
    Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, New York, USA
    Nat Immunol 10:531-9. 2009
    ..Thus, T cell antigen receptor signaling and the subsequent formation of immunological synapses are active processes dependent on myosin IIA...
  36. pmc Truncation of the catalytic domain of the cylindromatosis tumor suppressor impairs lung maturation
    Eirini Trompouki
    Institute of Immunology, Biomedical Sciences Research Center Al Fleming, 34 Al Fleming Str, Vari 16672, Greece
    Neoplasia 11:469-76. 2009
    ..and endothelial structures. Our study identifies an important role of CYLD in lung maturation, which may underlie the development of many cases of lung cancer...
  37. doi Cross-reactivity of autoantibodies from patients with epidermolysis bullosa acquisita with murine collagen VII
    Kinga Csorba
    Department of Dermatology, University of Freiburg, Freiburg, Germany
    Cell Mol Life Sci 67:1343-51. 2010
    ..used to characterize the reactivity of EBA sera and to map the epitopes on the murine antigen by ELISA and immunoblotting. The patients' autoantibody binding to murine collagen VII triggered pathogenic events as demonstrated by a ..
  38. ncbi hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase
    H Vaziri
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 107:149-59. 2001
    ..In contrast, expression of a catalytically inactive hSir2 protein potentiates p53-dependent apoptosis and radiosensitivity. We propose that hSir2 is involved in the regulation of p53 function via deacetylation...
  39. pmc Validity and reproducibility of measurement of islet autoreactivity by T-cell assays in subjects with early type 1 diabetes
    Kevan C Herold
    Yale University, New Haven, Connecticut, USA
    Diabetes 58:2588-95. 2009
    ..Such assays are vital for monitoring responses to interventions that may modulate disease progression...
  40. ncbi Bruton's tyrosine kinase and phospholipase Cgamma2 mediate chemokine-controlled B cell migration and homing
    David J J de Gorter
    Department of Pathology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
    Immunity 26:93-104. 2007
    ....
  41. doi Coronin-1a inhibits autophagosome formation around Mycobacterium tuberculosis-containing phagosomes and assists mycobacterial survival in macrophages
    Shintaro Seto
    Department of Infectious Diseases, Hamamatsu University School of Medicine, 1 20 1 Handa yama, Higashi ku, Hamamatsu 431 3192, Japan
    Cell Microbiol 14:710-27. 2012
    ..These results suggest that Coro1a inhibits autophagosome formation in alveolar macrophages, thereby facilitating M. tuberculosis survival within the lung...
  42. doi STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein
    Takashi Sato
    Department of Molecular Medicine, Global COE Cell Fate Regulation Research and Education Unit, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5 1 Oe honmachi, Kumamoto 862 0973, Japan
    Mol Cell 47:99-110. 2012
    ..Hence we postulate that STT3B-dependent posttranslational N-glycosylation is part of a triage-salvage system recognizing cryptic N-glycosylation sites of secretory proteins to preserve protein homeostasis...
  43. ncbi Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic response to Bortezomib, a novel proteasome inhibitor, in human H460 non-small cell lung cancer cells
    Yi He Ling
    Department of Oncology, Albert Einstein College of Medicine, Bronx, New York 10461, USA New York University Cancer Institute, School of Medicine, New York University, New York, New York 10016, USA
    J Biol Chem 278:33714-23. 2003
    ..Thus ROS generation plays a critical role in the initiation of the bortezomib-induced apoptotic cascade by mediation of the disruption of Delta psi m and the release of cytochrome c from mitochondria...
  44. doi NRF2 blockade suppresses colon tumor angiogenesis by inhibiting hypoxia-induced activation of HIF-1α
    Tae Hyoung Kim
    College of Pharmacy, Yeungnam University, Gyeongsangbuk do, South Korea
    Cancer Res 71:2260-75. 2011
    ....
  45. pmc Failure to activate the IFN-β promoter by a paramyxovirus lacking an interferon antagonist
    M J Killip
    School of Biology, Centre for Biomolecular Sciences, North Haugh, University of St Andrews, St Andrews, Fife, UK
    Virology 415:39-46. 2011
    ....
  46. ncbi Two distinct head-tail interfaces cooperate to suppress activation of vinculin by talin
    Daniel M Cohen
    Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 280:17109-17. 2005
    ..Together the D1-Vt and D4-Vt interfaces provide the high affinity (approximately 10(-9)) autoinhibition observed in full-length vinculin...
  47. ncbi Liver-specific deletion of the NADPH-cytochrome P450 reductase gene: impact on plasma cholesterol homeostasis and the function and regulation of microsomal cytochrome P450 and heme oxygenase
    Jun Gu
    Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York, Albany, New York 12201, USA
    J Biol Chem 278:25895-901. 2003
    ..The Alb-Cre/Cprlox mouse represents a unique model for studying the in vivo function of hepatic HO and microsomal CYP-dependent pathways in the biotransformation of endogenous and xenobiotic compounds...
  48. ncbi PKC-beta controls I kappa B kinase lipid raft recruitment and activation in response to BCR signaling
    Thomas T Su
    The Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Nat Immunol 3:780-6. 2002
    ..Together, these data define an essential role for PKC-beta in BCR survival signaling and highlight PKC-beta as a key therapeutic target for B-lineage malignancies...
  49. pmc Constitutively active Lck kinase in T cells drives antigen receptor signal transduction
    Konstantina Nika
    T Cell Signaling Laboratory, Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Immunity 32:766-77. 2010
    ..Our findings suggest a dynamic regulation of Lck activity that can be promptly utilized to initiate T cell activation and have implications for signaling by other immune receptors...
  50. ncbi Molecular partitioning during host cell penetration by Toxoplasma gondii
    Audra J Charron
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Traffic 5:855-67. 2004
    ..gondii PVM...
  51. ncbi Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    Navdar Sever
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 279:43136-47. 2004
    ..These results provide formal genetic proof for the essential role of Insig-1 in feedback control of lipid synthesis in cultured cells...
  52. pmc Caveolae participate in tumor necrosis factor receptor 1 signaling and internalization in a human endothelial cell line
    Alessio D'Alessio
    Interdepartmental Program in Vascular Biology and Transplantation, Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Ave, New Haven, CT 06536 0812, USA
    Am J Pathol 166:1273-82. 2005
    ....
  53. pmc RNA interference-mediated silencing of mitotic kinesin KIF14 disrupts cell cycle progression and induces cytokinesis failure
    Michael Carleton
    Rosetta Inpharmatics LLC, 401 Terry Ave, N Seattle, Washington 98109, USA
    Mol Cell Biol 26:3853-63. 2006
    ..Furthermore, the link we observed between siRNA efficacy and phenotypic outcome indicates that distinct stages during cell cycle progression are disrupted by the differential modulation of KIF14 expression...
  54. pmc JAM-A regulates epithelial proliferation through Akt/β-catenin signalling
    Porfirio Nava
    Epithelial Pathobiology Research Unit, Department of Pathology, Emory University, Whitehead Biomedical Research Building, Room 105E, 615 Michael Street, Atlanta, Georgia 30322, USA
    EMBO Rep 12:314-20. 2011
    ..Finally, inhibition of Akt reversed colonic crypt hyperproliferation in JAM-A-deficient mice. These data establish a new link between JAM-A and IEC homeostasis...
  55. pmc Variant-specific [PSI+] infection is transmitted by Sup35 polymers within [PSI+] aggregates with heterogeneous protein composition
    Sviatoslav N Bagriantsev
    Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA
    Mol Biol Cell 19:2433-43. 2008
    ..Hsp104, Sis1, and Sse1 interact preferentially with the prion versus nonprion form of Sup35, whereas Sla2 and Ssb1/2 interact with both forms of Sup35 with similar efficiency...
  56. pmc Sequence polymorphism, predicted secondary structures, and surface-exposed conformational epitopes of Campylobacter major outer membrane protein
    Q Zhang
    Food Animal Health Research Program, The Ohio State University, Wooster, Ohio 44691, USA
    Infect Immun 68:5679-89. 2000
    ..These findings are instrumental in the design of MOMP-based diagnostic tools and vaccines...
  57. pmc Treating metastatic solid tumors with bortezomib and a tumor necrosis factor-related apoptosis-inducing ligand receptor agonist antibody
    Anil Shanker
    Laboratory of Experimental Immunology, Cancer and Inflammation Program, SAIC Frederick, Inc, National Cancer Institute Frederick, Frederick, MD 21702, USA
    J Natl Cancer Inst 100:649-62. 2008
    ..All statistical tests were two-sided...
  58. ncbi Functional compensation for adipose differentiation-related protein (ADFP) by Tip47 in an ADFP null embryonic cell line
    Carole Sztalryd
    Geriatric Research, Education and Clinical Center, Baltimore Veterans Affairs Health Care Center, Department of Medicine, School of Medicine, University of Maryland, Baltimore, Maryland 21201, USA
    J Biol Chem 281:34341-8. 2006
    ..sole lipid droplet-associated protein from the PAT family by mass spectroscopy, which was further confirmed by immunoblotting and immunocytochemistry...
  59. ncbi Binding of zinc finger protein ZPR1 to the epidermal growth factor receptor
    Z Galcheva-Gargova
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, 01605, USA
    Science 272:1797-802. 1996
    ..The effect of EGF to regulate ZPR1 binding is dependent on tyrosine phosphorylation of the EGFR. ZPR1 therefore represents a prototype for a class of molecule that binds to the EGFR and is released from the receptor after activation...
  60. pmc O-linked β-N-acetylglucosamine transferase is indispensable in the failing heart
    Lewis J Watson
    Department of Medicine, Institute of Molecular Cardiology, University of Louisville, Louisville, KY 40202, USA
    Proc Natl Acad Sci U S A 107:17797-802. 2010
    ..These data provide keen insights into the pathophysiology of the failing heart and illuminate a previously unrecognized point of integration between metabolism and cardiac function in the failing heart...
  61. ncbi Nephroblastoma overexpressed/cysteine-rich protein 61/connective tissue growth factor/nephroblastoma overexpressed gene-3 (NOV/CCN3), a selective adrenocortical cell proapoptotic factor, is down-regulated in childhood adrenocortical tumors
    Mabrouka Doghman
    Institut de Pharmacologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique Unité Mixte de Recherche 6097, Universite de Nice Sophia Antipolis, 06560 Valbonne, France
    J Clin Endocrinol Metab 92:3253-60. 2007
    ..Nephroblastoma overexpressed (NOV)/cysteine-rich protein 61/connective tissue growth factor/nephroblastoma overexpressed gene-3 mRNA is significantly down-regulated in childhood ACTs...
  62. ncbi Regulation of sphingosine 1-phosphate-induced endothelial cytoskeletal rearrangement and barrier enhancement by S1P1 receptor, PI3 kinase, Tiam1/Rac1, and alpha-actinin
    Patrick A Singleton
    Division of Pulmonary and Critical Care Medicine, Center for Translational Respiratory Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    FASEB J 19:1646-56. 2005
    ..b>Immunoblotting from S1P-treated EC revealed S1P-mediated rapid recruitment (1 microM, 5 min) to CEMs of the S1P receptors ..
  63. pmc The transcription factor ZEB1 (deltaEF1) promotes tumour cell dedifferentiation by repressing master regulators of epithelial polarity
    K Aigner
    Max F Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
    Oncogene 26:6979-88. 2007
    ..In invasive ductal and lobular breast cancer, upregulation of ZEB1 was stringently coupled to cancer cell dedifferentiation. Our data show that ZEB1 represents a key player in pathologic EMTs associated with tumour progression...
  64. doi RKTG sequesters B-Raf to the Golgi apparatus and inhibits the proliferation and tumorigenicity of human malignant melanoma cells
    Fengjuan Fan
    Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China
    Carcinogenesis 29:1157-63. 2008
    ..Collectively, our results suggest that RKTG may play a suppressive role in human melanoma that harbors an oncogenic B-Raf mutation via its antagonistic action on B-Raf...
  65. ncbi Monolysocardiolipins accumulate in Barth syndrome but do not lead to enhanced apoptosis
    Fredoen Valianpour
    Laboratory of Genetic Metabolic Diseases, Department of Clinical Chemistry, Emma Children s Hospital, Academic Medical Center, University of Amsterdam, 1100 DE Amsterdam, The Netherlands
    J Lipid Res 46:1182-95. 2005
    ....
  66. pmc SIRT3 deacetylates mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase 2 and regulates ketone body production
    Tadahiro Shimazu
    Gladstone Institute of Virology and Immunology, San Francisco, CA 94158, USA
    Cell Metab 12:654-61. 2010
    ..Our findings show SIRT3 regulates ketone body production during fasting and provide molecular insight into how protein acetylation can regulate enzymatic activity...
  67. ncbi Ubiquitination of p27Kip1 requires physical interaction with cyclin E and probable phosphate recognition by SKP2
    Dana Ungermannova
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA
    J Biol Chem 280:30301-9. 2005
    ..Thus, our data unravel the molecular organization of the ubiquitination complex that catalyzes p27 ubiquitination and provide unique insights into the specificity of substrate recognition by SCF(Skp2)...
  68. ncbi Distribution of GLUT3 glucose transporter protein in human tissues
    P R Shepherd
    Charles A Dana Research Institute, Beth Israel Hospital, Boston, MA 02215
    Biochem Biophys Res Commun 188:149-54. 1992
    ..No GLUT3 was detected in membranes from any of 3 skeletal muscle groups investigated. We conclude that a major role of GLUT3 in humans is as the brain neuronal glucose transporter...
  69. doi The rules of DNA recognition by the androgen receptor
    Sarah Denayer
    Katholieke Universiteit Leuven, Campus Gasthuisberg, O N1, Herestraat 49, 3000 Leuven, Belgium
    Mol Endocrinol 24:898-913. 2010
    ..So far, all AR-binding genomic fragments that were analyzed contain AREs defined as receptor-dimer binding motifs with the ability to confer responsiveness to a reporter gene...
  70. ncbi CaT1 contributes to the stores-operated calcium current in Jurkat T-lymphocytes
    Jie Cui
    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 277:47175-83. 2002
    ..Taken together these results indicate that CaT1 is the channel protein that contributes to T-lymphocyte SOCCs either alone or as a subunit in a heterogeneous channel complex...
  71. ncbi UV-induced ataxia-telangiectasia-mutated and Rad3-related (ATR) activation requires replication stress
    Irene M Ward
    Guggenheim 1306, Division of Oncology Research, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 279:9677-80. 2004
    ..Phosphorylated H2AX might further facilitate ATR activation by stabilizing ATR at the sites of arrested replication forks...
  72. ncbi Dopamine D5 receptors of rat and human brain
    Z U Khan
    Department of Cell Biology, Faculty of Sciences, University of Malaga, Teatinos 29071, Malaga, Spain
    Neuroscience 100:689-99. 2000
    ..Furthermore, the presence of D5 receptors in the areas of dopamine pathways suggests that this receptor may participate actively in dopaminergic neurotransmission...
  73. ncbi A phosphorylation state-specific antibody recognizes Hsp27, a novel substrate of protein kinase D
    Heike Döppler
    Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 280:15013-9. 2005
    ..We term this antibody anti-PKD pMOTIF and predict that it will enable the discovery of novel PKD substrate proteins in cells...
  74. pmc Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues
    Aldrin V Gomes
    Department of Physiology and Medicine, Cardiac Proteomics and Signaling Laboratory at Cardiovascular Research Laboratory, University of California Los Angeles, School of Medicine, Los Angeles, CA 90095, USA
    Mol Cell Proteomics 8:302-15. 2009
    ..The functional heterogeneity of the mammalian 20 S proteasome complexes underscores the concept of divergent proteomes among organs in the context of an identical genome...
  75. doi Human Merkel cell polyomavirus infection I. MCV T antigen expression in Merkel cell carcinoma, lymphoid tissues and lymphoid tumors
    Masahiro Shuda
    Molecular Virology Program, University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
    Int J Cancer 125:1243-9. 2009
    ..As with some of the other human polyomaviruses, lymphocytes may serve as a tissue reservoir for MCV infection, but hematolymphoid malignancies associated with MCC are unlikely to be caused by MCV...
  76. pmc Arabidopsis STN7 kinase provides a link between short- and long-term photosynthetic acclimation
    Paolo Pesaresi
    Dipartimento di Produzione Vegetale, Università degli studi di Milano c o Parco Tecnologico Padano Via Einstein, 26900 Lodi, Italy
    Plant Cell 21:2402-23. 2009
    ....
  77. pmc WNK kinases regulate thiazide-sensitive Na-Cl cotransport
    Chao Ling Yang
    Division of Nephrology and Hypertension, Department of Medicine, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Clin Invest 111:1039-45. 2003
    ..Together, these results identify WNK kinases as a previously unrecognized sodium regulatory pathway of the distal nephron. This pathway likely contributes to normal and pathological blood pressure homeostasis...
  78. ncbi The fasting-induced adipose factor/angiopoietin-like protein 4 is physically associated with lipoproteins and governs plasma lipid levels and adiposity
    Stéphane Mandard
    Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University, The Netherlands
    J Biol Chem 281:934-44. 2006
    ..Our data indicate that disturbances in FIAF signaling might be involved in dyslipidemia...
  79. ncbi BAD Ser-155 phosphorylation regulates BAD/Bcl-XL interaction and cell survival
    Y Tan
    Cell Signaling Technology, Beverly, MA 01915, USA
    J Biol Chem 275:25865-9. 2000
    ..RSK1 phosphorylates BAD at both Ser-112 and Ser-155 and rescues BAD-mediated cell death in a manner dependent upon phosphorylation at both sites...
  80. pmc Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily
    P F Jones
    Friedrich Miescher Institut, Basel, Switzerland
    Proc Natl Acad Sci U S A 88:4171-5. 1991
    ..Specific antisera directed towards the carboxyl terminal of the rac protein kinase were prepared and used to identify that phosphorylated several substrates in immunoprecipitates prepared with the rac-specific antisera...
  81. ncbi Tyrosine phosphorylation of the beta-amyloid precursor protein cytoplasmic tail promotes interaction with Shc
    Philip E Tarr
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 277:16798-804. 2002
    ..Thus, APP and Shc proteins interact in vitro, in cells, and in the mouse brain. Tyrosine phosphorylation of APP may promote the interaction with Shc proteins...
  82. ncbi ABAD enhances Abeta-induced cell stress via mitochondrial dysfunction
    Kazuhiro Takuma
    Departments of Surgery, Pathology, and Neurology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    FASEB J 19:597-8. 2005
    ....
  83. doi Phosphorylated TDP-43 in Alzheimer's disease and dementia with Lewy bodies
    Tetsuaki Arai
    Department of Psychogeriatrics, Tokyo Institute of Psychiatry, Tokyo Metropolitan Organization for Medical Research, 2 1 8 Kamikitazawa, Setagaya Ku, Tokyo 156 8585, Japan
    Acta Neuropathol 117:125-36. 2009
    ..There may be genetic factors, such as mutations or genetic variants of PGRN underlying the co-occurrence of abnormal deposition of TDP-43, tau and alpha-synuclein...
  84. doi The E3 ubiquitin ligase Nrdp1 'preferentially' promotes TLR-mediated production of type I interferon
    Chen Wang
    National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai, China
    Nat Immunol 10:744-52. 2009
    ..Our data suggest that Nrdp1 functions as both an adaptor protein and an E3 unbiquitin ligase to regulate TLR responses in different ways...
  85. ncbi An androgen receptor NH2-terminal conserved motif interacts with the COOH terminus of the Hsp70-interacting protein (CHIP)
    Bin He
    Laboratories for Reproductive Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 279:30643-53. 2004
    ....
  86. ncbi Dynamics of the p53-Mdm2 feedback loop in individual cells
    Galit Lahav
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Genet 36:147-50. 2004
    ..This approach can be used to study other signaling systems and suggests that the p53-Mdm2 feedback loop generates a 'digital' clock that releases well-timed quanta of p53 until damage is repaired or the cell dies...
  87. pmc Activation of innate immune antiviral responses by Nod2
    Ahmed Sabbah
    Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
    Nat Immunol 10:1073-80. 2009
    ..Thus, the function of Nod2 as a viral PRR highlights the important function of Nod2 in host antiviral defense mechanisms...
  88. pmc Effect of disrupting seven-in-absentia homolog 2 function on lung cancer cell growth
    Atique U Ahmed
    Departments of Surgery and Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Natl Cancer Inst 100:1606-29. 2008
    ..We examined whether inhibiting SIAH-2 function blocks lung cancer growth...
  89. ncbi The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases
    Rebecca L Hurley
    Departments of Medicine and Biochemistry, Dartmouth Medical School and the Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA
    J Biol Chem 280:29060-6. 2005
    ..These data indicate that the CaMKKs function in intact cells as AMPKKs, predicting wider roles for these kinases in regulating AMPK activity in vivo...
  90. pmc ATR-mediated checkpoint pathways regulate phosphorylation and activation of human Chk1
    H Zhao
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110 1093, USA
    Mol Cell Biol 21:4129-39. 2001
    ..In addition, this study implicates ATR as a direct upstream activator of Chk1 in human cells...
  91. pmc An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker a motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens
    Lay Sun Ma
    Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
    J Bacteriol 191:4316-29. 2009
    ..In conclusion, ImpL(M) interacts with ImpK(L), and its Walker A motif is required for its function in mediation of Hcp secretion from A. tumefaciens...
  92. pmc Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells
    M Furuse
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 153:263-72. 2001
    ....
  93. pmc YscP and YscU switch the substrate specificity of the Yersinia type III secretion system by regulating export of the inner rod protein YscI
    Sarah E Wood
    Department of Pediatrics, Center for Vaccine Development, University of Maryland at Baltimore, 685 W Baltimore St, Baltimore, MD 21201, USA
    J Bacteriol 190:4252-62. 2008
    ..Together, these results suggest that the formation of the inner rod, not the needle, is critical for substrate specificity switching and that YscP and YscU exert their effects on substrate export by controlling the secretion of YscI...
  94. ncbi Caveolin-1 interacts with 5-HT2A serotonin receptors and profoundly modulates the signaling of selected Galphaq-coupled protein receptors
    Anushree Bhatnagar
    Department of Biochemistry, Case Western Reserve University School of Medicine, 2109 Adelbert Road, Cleveland, OH 44106, USA
    J Biol Chem 279:34614-23. 2004
    ..These studies provide compelling evidence for a prominent role of Cav-1 in regulating the functional activity of not only 5-HT(2A) serotonin receptors but also selected Galpha(q)-coupled receptors...
  95. pmc Mammalian target of rapamycin protein complex 2 regulates differentiation of Th1 and Th2 cell subsets via distinct signaling pathways
    Keunwook Lee
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Immunity 32:743-53. 2010
    ..Collectively, the data uncover vital mTOR-PKC and mTOR-Akt connections in T cell differentiation and reveal distinct pathways by which mTORC2 regulates development of Th1 and Th2 cell subsets...
  96. ncbi NF-kappa B as a therapeutic target in multiple myeloma
    Teru Hideshima
    Jerome Lipper Multiple Myeloma Center, Dana Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:16639-47. 2002
    ..Furthermore, they provide the framework for clinical evaluation of novel MM therapies based upon targeting NF-kappaB...
  97. ncbi COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence
    Suchithra Menon
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06511, USA
    Nat Immunol 8:1236-45. 2007
    ..Our data define a CSN-dependent pathway of transcriptional control that is essential for antigen-induced initiation of T cell proliferation...
  98. ncbi Insig-dependent ubiquitination and degradation of mammalian 3-hydroxy-3-methylglutaryl-CoA reductase stimulated by sterols and geranylgeraniol
    Navdar Sever
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 278:52479-90. 2003
    ....
  99. ncbi Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the severe-acute respiratory syndrome-coronavirus (SARS-CoV) receptor, angiotensin-converting enzyme-2 (ACE2)
    Daniel W Lambert
    Proteolysis Research Group, School of Biochemistry and Microbiology, University of Leeds, Leeds LS2 9JT, United Kingdom
    J Biol Chem 280:30113-9. 2005
    ..The identification of ADAM17 as the protease responsible for ACE2 shedding may provide new insight into the physiological roles of ACE2...
  100. ncbi Steroid 5alpha-reductase isozymes I and II in recurrent prostate cancer
    Mark A Titus
    Department of Pathology and Laboratory Medicine, Surgery, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7295, USA
    Clin Cancer Res 11:4365-71. 2005
    ..The aim of this study was to characterize steroid 5alpha-reductase isozymes I (S5alphaRI) and II (S5alphaRII) in prostate tissues...
  101. pmc Sequential phosphorylation of CCAAT enhancer-binding protein beta by MAPK and glycogen synthase kinase 3beta is required for adipogenesis
    Qi Qun Tang
    Departments of Pediatrics Division of Endocrinology, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 102:9766-71. 2005
    ..The delayed transactivation of the C/EBPalpha and PPARgamma genes by C/EBPbeta appears necessary to allow mitotic clonal expansion, which would otherwise be prevented, because C/EBPalpha and PPARgamma are antimitotic...

Research Grants75

  1. Inhibition of human neutrophil function by Francisella tularensis
    Lee Ann H Allen; Fiscal Year: 2012
    ..electron microscopy to quantify phagosome escape and PMN viability;in vitro kinase assays and immunoblotting to measure intracellular signaling;DNA microarrays to define changes in neutrophil gene expression;and both ..
  2. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
    ..Data will also be analyzed using biochemical immunoblotting and QRT-PCR techniques and rigorously quantified using imaging software, image segmentation and computational ..
  3. Axonopathy in Glaucoma
    Samuel D Crish; Fiscal Year: 2013
    ..Using a combination of immunoblotting, tract tracing, histology, and quantitative immunochemistry this research enterprise will seek to determine ..
  4. Ferritin: Protein/mRNA/DNA in Fe/O Regulation/Metabolism
    ELIZABETH C contact THEIL; Fiscal Year: 2010
    ..in vitro translation, mutagenesis, protein expression, EMSA, DNA-5mC analysis), and Cell Biology (RT-PCR, immunoblotting)...
  5. The Role of Adipocyte-Induced Inflammation in Prostate Tumor Progression
    AIMALIE LYNNETTE HARDAWAY; Fiscal Year: 2012
    ..including: tumor cell-adipocyte-macrophage co-culture system, in vivo knockout model of bone metastasis, immunoblotting, ELISA assays, cytokine arrays, RT PCR and Lentivirus knockdown technology...
  6. Iron, NO, and Lipid Peroxide in Photodynamic Therapy
    ALBERT GIROTTI; Fiscal Year: 2013
    ..and apoptosis evaluation;use of NOS inhibitors, NO scavengers, and chemical NO donors;RNA interference;immunoblotting;confocal microscopy;and PDT of human tumors implanted in immunosuppressed mice;in addition to ALA-PDT, ..
  7. Signal Transduction Mechanism of Osteoclast Differentiation
    Nandini Ghosh-Choudhury; Fiscal Year: 2013
    ..of BMP-2 in activating these important transcription factors leading to osteoclast differentiation using immunoblotting, immunohistochemistry, in situ hybridization, ChIP analysis and qRTPCR techniques...
  8. Role of G-gamma-7 in striatal cannabinoid and D2 dopamine
    Dana E Selley; Fiscal Year: 2010
    ..Whether any reduction in G-protein activation is due to a loss in receptor expression will be tested by immunoblotting of CB1 and D2 receptors...
  9. Dopamine Transporter Structure and Function
    Christopher K Surratt; Fiscal Year: 2010
    ..surface expression and accessibility of introduced cysteine residues of the mutant transporter proteins via immunoblotting and cysteine alkylation experiments, and immunochemical analysis of DAT proteolysates following covalent ..
  10. Epigenetic regulation of stem cell senescence in aging
    DAVID TOCHUKWU ASUZU; Fiscal Year: 2011
    ..and the role of Wnt signaling and its epigenetic control by polycomb proteins using real-time RT-PCR, Western immunoblotting and RNA interference...
  11. Role of the Renin-Angiotensin system in placental insufficiency
    Eugenia Mata-Greenwood; Fiscal Year: 2013
    ..The expression of RAS members will be determined by real-time PCR and immunoblotting. ACE and ACE2 enzymatic activity and Angiotensin II and 1-7 tissue levels will be assessed by EIA...
  12. Mechanisms of Arsenic-Induced Developmental Toxicity
    Lisa Bain; Fiscal Year: 2009
    ..multinucleation, muscle-specific genes, and myogenic transcription factor expression by real-time PCR and immunoblotting. To investigate the mechanisms responsible for the lack of differentiation, DNA methylation, methyltransferase ..
  13. Epithelial Sodium Channels in Vasopressin and Oxytocin Synthesizing Magnocellular
    Ryoichi Teruyama; Fiscal Year: 2013
    ..employ the whole-cell patch clamp technique combined with immunocytochemistry, semi-quantitative RT-PCR and immunoblotting to determine: 1) how dietary salt intake affects both ENaC activity and the neuronal activity in MNCs;2) the ..
  14. Dietary HDAC Inhibitors in Colon Cancer Prevention
    Roderick H Dashwood; Fiscal Year: 2012
    ..Using immunoblotting, ChIP/re-ChIP, qRT-PCR and siRNA knockdown, systematically examine HDACs associated with the promoter region ..
  15. Mechanism of Renal Cell Injury in Diabetes
    Goutam Ghosh Choudhury; Fiscal Year: 2013
    ..To address these specific aims, techniques including immunoprecipitation, immunoblotting, morphometry, immunohistochemistry, reporter transfection assays, chromatin immunoprecipitation assays, siRNA-..
  16. Gene therapy in the cornea
    RAJIV RAVINDRA MOHAN; Fiscal Year: 2012
    ..and techniques, including slit-lamp, stereomicroscope and optical coherence tomography, immunohistochemistry, immunoblotting, quantitative PCR, southern blot, transmission electron and confocal microscopy, etc...
  17. Obesity Development: Role of Air Pollution and High Fat Diet
    Qinghua Sun; Fiscal Year: 2010
    ..tolerance test, magnetic resonance imaging on body fat mass, myograph on vasomotor tone change, morphology and immunoblotting of skeletal muscle, liver, subcutaneous and visceral adipose tissues on IR, and adipocyte number and size ..
  18. The role of phosphorylation of Ulk1 by GSK-3b in myocardial autophagy and aging
    Peiyong Zhai; Fiscal Year: 2013
    ..b>Immunoblotting of aging marker will also be carried out...
  19. Astrocyte Function and Temporal Lobe Epilepsy
    JAY ROBERT VARGAS; Fiscal Year: 2010
    ..Specific Aim 1 ofthe proposal will use immunohistochemistry and immunoblotting techniques to determine if SE results in a long- term increase in the expression of KAR in astrocytes...
  20. Regulation of a Novel Trypanosome RNA Binding Protein by Arginine Methylation
    Kaylen Lott; Fiscal Year: 2013
    ..In Aim 2, we will determine TbMiz1 subcellular localization by immunoblotting and immunofluorescence and compare localization of the hypomethylated mutant TbMiz1...
  21. Regulation of Angiogenesis by Kininogen
    Keith R McCrae; Fiscal Year: 2013
    ..Screening of mKng1-/- mice by immunoblotting using several different kininogen antibodies as well as a sensitive BK radioimmunoassay, demonstrates that ..
  22. cGMP manufacturing of PBI-220, a broad spectrum immunoadhesin therapeutic against
    LLOYD M YU; Fiscal Year: 2013
    ..Product identity, strength, potency, purity and stability will be evaluated using SDS-PAGE and immunoblotting, isoelectric focusing, UV spectroscopy, binding assays, size exclusion and peptide mapping liquid ..
  23. SIGNALING MECHANISMS IN REGULATION OF TUMOR ANGIOGENESIS
    DOMINIC E COSGROVE; Fiscal Year: 2012
    ..We will analyze these models using immunoblotting, co-immunoprecipitation, immunohistochemistry, Matrigel plug, and tumor studies...
  24. The Role of the DNA Unwinding Element Binding Protein, DUE-B, in DNA Replication
    Michael Leffak; Fiscal Year: 2010
    ..Our major analytical techniques will be immunoblotting, chromatin immunoprecipitation, quantitative PCR, FRET, and immunofluorescence...
  25. Muscle protein synthesis and gene expression in myostatin-deficient mice
    Stephen L Welle; Fiscal Year: 2010
    ..Concentrations and phosphorylation status of selected proteins will be determined by immunoblotting. Immunohistochemistry will be used to assess muscle fiber hypertrophy, fiber types, numbers of fibers, and ..
  26. Molecular Regulation of GABA(A) Receptor Function in Amygdala
    Kerry J Ressler; Fiscal Year: 2010
    ..this hypothesis including: 1) examination of GABAA complex genes and proteins with in situ hybridization and immunoblotting;2) manipulation of GABAA receptor expression via amygdala-specific deletion of the GABAA alpha1 gene using the ..
  27. Role of Calpastatin in mutant SOD1-mediated motor neuron disease
    Mala V Rao; Fiscal Year: 2010
    ..We will use mouse breeding techniques, Southern Blotting, polymerase chain reactions, immunoblotting, immunocytochemistry, electron microscopy and Rotarod tests to achieve our goals...
  28. An Attenuated E.coli Vaccine for Enterotoxigenic E.coli (ETEC)
    Edgar C Boedeker; Fiscal Year: 2010
    ..these ends, the expression of ETEC antigens by the vaccine constructs will be demonstrated by SDS-PAGE, immunoblotting, and bacterial agglutination...
  29. Perinatal choline therapy in a mouse model of Down Syndrome &Alzheimer's Disease
    Barbara J Strupp; Fiscal Year: 2012
    ..Levels of these neurotrophins and receptors will be measured using immunoblotting and correlated with measures of memory and attention from these same subjects to establish structure-function ..
  30. Does Demethylation of the Inactive X Contribute to Lupus in Women?
    Bruce C Richardson; Fiscal Year: 2013
    ..the consequences of OGT overexpression on CD4+ T cell gene expression using transfections, microarrays and immunoblotting, and identify the miRNA target transcripts using Argonaute pulldowns followed by array analyses or sequencing, ..
  31. The Cutaneous Biology of MAGE Transcription Factors
    B Jack Longley; Fiscal Year: 2013
    ..KAP1- KRAB binding in a mammalian two hybrid assay, b. KAP1 mediated gene repression in a luciferase reporter gene assay, and c. KAP1 mediated KZNF ubiquitination by immunoblotting.
  32. Smooth Muscle Cell Protein Serotonylation and Pulmonary Hypertension
    BARRY FANBURG; Fiscal Year: 2013
    ..migration and development of PH with a variety of cell culture, transfection, siRNA, immunoprecipitation and immunoblotting techniques and with the use of normal and transgenic rodent model experiments...
  33. Interplay between Chromatin and Co-activator Complexes
    Michael F Carey; Fiscal Year: 2012
    ..are attached to streptavidin-coated beads to capture the PICs from extracts and analyze their composition by immunoblotting. The functions of the PIC are studied using histone modification/remodeling assays and in vitro transcription...
  34. Actin dynamics and regulation of epithelial barrier in intestinal inflammation
    ANDREI IVANOVICH IVANOV; Fiscal Year: 2013
    ..and cross-linking proteins will be analyzed by a combination of biochemical (actin- monomer sequestration, immunoblotting, detergent fractionation), immunocytochemical and genetic (siRNA-mediated knock-downs, expression of dominant-..
  35. Signal Transduction of Human Keratinocyte Migration
    Wei Li; Fiscal Year: 2010
    ..and secretion in HKs, but not in dermal fibroblasts (DFs);3) Comparison of human skin tissue staining and immunoblotting lysates of isolated human skin cells with anti-hsp90 antibodies suggests that extracellular hsp90alpha pre-..
  36. The role of PI3K/Akt pathway in the pathogenesis and treatment of Krabbe disease
    TUBA FEHR; Fiscal Year: 2012
    ..effect by adding varying concentrations of psychosine in VEGF-induced motorneuron-like cells (NSC34) by immunoblotting and immunohistochemistry...
  37. Mechanisms of Complement Evasion by Treponema denticola
    DANIEL PATRICK MILLER; Fiscal Year: 2013
    ..biochemical approaches such as recombinant protein expression and purification, surface plasmon resonance, immunoblotting and allelic exchange to determine if protein sequence variation affects the FhbB:FH interaction and protective ..
  38. BH4 in hyperoxic lung injury in newborn
    Ru Jeng Teng; Fiscal Year: 2013
    ..by chromatin immunoprecipitation, DNA methylation, quantitative real-time polymerase chain reaction, and immunoblotting. Studies under specific aim 2 will investigate the mechanistic link between BH4 depletion and hyperoxia-..
  39. Modulation of MUC1 Mucin Trafficking
    Rebecca P Hughey; Fiscal Year: 2010
    ..We will use a combination of metabolic labeling, surface biotinylation and immunoblotting of endogenous EGFR and MUC1 and recombinant mutants in normal and glycosylation-defective MDCK cells to ..
  40. The effect of HIV on apoptosis and cellular proliferation in laryngeal papillomas
    Michael P Underbrink; Fiscal Year: 2013
    ..Aim 1 of the research project is to use immunoblotting and shRNA knockdown to determine the ability of HPV6 and HPV11 E6 proteins to blunt the apoptotic response, ..
  41. Mechanism of Renal Cell Injury
    Goutam Ghosh Choudhury; Fiscal Year: 2013
    ..these specific aims, techniques including immunohistochemistry/immunofluorescence, immunoprecipitation, immunoblotting, reporter transfection assays, electrophoretic mobility shift assays, chromatin immunoprecipitation asays, ..
  42. Vitamin D Analogs as Adjuvants in Chemotherapy of Cancer
    George P Studzinski; Fiscal Year: 2013
    ..plasmid constructs to study the molecular consequences of these manipulations, which will be determined by immunoblotting, quantitative RT-PCR, immunoprecipitation, and other molecular techniques...
  43. The Role of Ubiquitin in Sodium Chloride Co-Transporter Regulation
    BENJAMIN S KO; Fiscal Year: 2012
    ..surface expression via biotinylation and immunofluorescence, and ubiquitination via immunoprecipitation and immunoblotting in cells gene-silenced for WNK4 or treated with phorbol esters...
  44. Ethanol Regulation of Neuronal Nicotinic Receptor Expression in Reward Circuitry
    Andrew R Tapper; Fiscal Year: 2010
    ..Quantitative immunoblotting will also be used to measure changes in nAChR protein expression with alcohol exposure...
  45. IMMATURE HIPPOCAMPUS: EPILEPTOGENIC PROPERTIES
    JOHN WILLIAM SWANN; Fiscal Year: 2012
    ..b>Immunoblotting for dendritic proteins as well as dendrite reconstructions at numerous developmental time points will attempt ..
  46. In Vivo Neuronal Modulation via Engineered G Protein-Coupled Receptors
    SARAH CLAIRE ROGAN; Fiscal Year: 2012
    ..used, including immunohistochemistry, in vivo microdialysis, measurement of inositol phosphate accumulation, immunoblotting, and stereotaxic viral infusion...
  47. CaMKII as a Mechanism &Intervention Target for Sickle Cell Pain
    Zaijie Jim Wang; Fiscal Year: 2013
    ..We will use real time PCR, immunoblotting, immunohisto-chemistry, and enzymatic kinetics methods to systematically examine the expression and activity ..
  48. Mechanisms Mediating Age-Related Impairments in Microvascular Collaterals
    STEVEN JEFFREY MILLER; Fiscal Year: 2012
    ..Protein and metabolite expression analysis with immunoblotting and HPLC will be used to identify molecules that regulate NO and reactive oxygen species (ROS) during aging...
  49. Smad4 promotes intestinal homeostasis through suppression of Wnt signaling
    Tanner J Freeman; Fiscal Year: 2013
    ..We will confirm loss of Smad4 expression by immunoblotting, IF and qPCR...
  50. Hypothermia enhances inflammatory cytokine expression via NF-kappa B
    Karen D Fairchild; Fiscal Year: 2010
    ..phosphorylation and acetylation events contribute to the HT effect on pro-inflammatory cytokines, through immunoblotting, in vitro phosphatase and deacetylase assays, and RNA interference...
  51. Molecular mechanism of LPA3-mediated uterine receptivity
    Xiaoqin Ye; Fiscal Year: 2013
    ..Laser microdissection, gene profiling, immunoblotting, ChIP assay, and immonoprecipitation are among the approaches that will be employed...
  52. Epithelial Na Channels and Regulation of Na Excretion
    Lawrence G Palmer; Fiscal Year: 2012
    ..We will use a combination of electrophysiological techniques and immunoblotting using antibodies specific for the 3 channel subunits alpha, beta and gamma ENaC to examine changes in the ..
  53. Calcium-Independent PLA2Beta in Beta-Cell Apoptosis
    Sasanka Ramanadham; Fiscal Year: 2013
    ..Apoptosis, flow cytometry, immunoblotting, real time-PCR, enzymatic activity, confocal microscopy, molecular biology, and mass spectrometry protocols ..
  54. Role of mitochondria in cardiac protection
    Peipei Ping; Fiscal Year: 2010
    ..isoforms of VDAC (VDAC1-3) and ANT (ANT1-2) are expressed in the adult mouse heart as detected by both immunoblotting (using antibodies we developed since the original submission) and LC/MS/MS...
  55. CHD1, Chromatin Dynamics, and Salivary Gland Differentiation
    JOSEPH ADAM HALL; Fiscal Year: 2010
    ..NFS-sId and control NFS-N mice will be utilized for all experiments. Gene expression and immunoblotting assays, combined with in vivo immunoprecipitation experiments, will shed light on the influence of quantity, ..
  56. Molecular Mechanisms Linking Placental Nutrient Sensing and Fetal Programming
    Thomas Jansson; Fiscal Year: 2013
    ..cells will be incubated in trophoblast CM and secretion and phosphorylation of IGFBP-1 (Western blot, 2-D immunoblotting, ELISA and mass spectrometry approaches) as well as IGF-I secretion (ELISA) will be determined...
  57. Regulation of eNOS by Shear Stress in Intact Arteries
    Robin C Looft Wilson; Fiscal Year: 2010
    ..inhibitors), at sequential time-points, and analyzed for phosphorylation and protein-protein interaction using immunoblotting and immunoprecipitation...
  58. Antigen-microarray validated monoclonal antibody library for analysis of brain-ex
    Seth Blackshaw; Fiscal Year: 2010
    ..of protein research because of their use in immunohistochemistry, (co)immunoprecipitation, ChIP on chip, immunoblotting and many other methods...
  59. Cav1.2 as a novel target for cocaine addiction pharmacotherapy
    ANNI SEUNGEUN LEE; Fiscal Year: 2013
    ..I will then use immunoblotting to measure the amount of HDAC5, phospho-HDAC5 and phosphorylated CaMKII, a protein which acts on HDAC5, in ..
  60. Mesenchymal stromal cells and bronchopulmonary dysplasia
    ANTONIA PETROVA POPOVA; Fiscal Year: 2013
    ..Stromal cell mRNA and protein expression will be measured by microarray and ELISA/immunoblotting. Training Objective: Develop experience and understanding in experimental design, informed consent, and ..
  61. Mfge8 and the Role of Apoptotic Cell Clearance in Lung Injury
    Kamran Atabai; Fiscal Year: 2011
    ..proteins, immunohistochemistry, immunocytochemistry, DNA expression arrays, Real-Time PCR, ELISA, and immunoblotting. Dr. Atabai's mentor, Dr...
  62. The Functions of IFITM Proteins in Control of Influenza A Virus Infection
    I Chueh Huang; Fiscal Year: 2013
    ..In this aim, a series of immunoprecipitatio, immunoblotting, drug inhibitory, and viral entry assays will be performed to determine the pathway contributing to IFITM3 ..
  63. Bile acid-induced colon cancer cell proliferation
    Jean Pierre Raufman; Fiscal Year: 2009
    ..1b. The mechanism of cholinergic agonist-induced cell proliferation will be studied using immunoblotting to probe for activated proteins in the p44/42 MARK, p38 MARK and JNK pathways, and by examining the roles of ..
  64. Perlecan modulates VEGF Bio-Availability in Human Osteoarthritic Cartilage
    RONALD GOMES; Fiscal Year: 2009
    ..HPLC and immunoblotting approaches already will then be employed to characterize the disaccharide composition of perlecan...
  65. VON WILLEBRAND FACTOR, FIBRINOLYSIS, & THE ENDOTHELIUM
    Karen Hamilton; Fiscal Year: 1991
    ..The vWF multimer distribution will be evaluated by immunoblotting of agarose gels and compared to that of plasma vWF...
  66. MOLECULAR CLONING OF A NOVEL BASEMENT MEMBRANE COMPONENT
    Lawrence Chan; Fiscal Year: 1999
    ..The purified fractions will be analyzed by 2-dimensional gel electrophoresis and immunoblotting. The internal amino acid sequences will be obtained after proteolytic digestion of th purified protein...
  67. CA2+ RELEASE CHANNEL FROM CARDIAC SARCOPLASMIC RETICULUM
    Kevin Campbell; Fiscal Year: 1991
    ..Structural characterization of the ryanodine receptor will involve immunoblotting of cardiac sarcoplasmic reticulum following proteolytic and glycosidase treatments...
  68. Nutritional Biochemistry of beta-Carotene Dioxygenase
    A Andersson; Fiscal Year: 2005
    ..These analyses will be accomplished using immunoblotting, immunohistochemistry, RNA blotting, in situ mRNA hybridization, and BCDO enzyme assays...
  69. Monoamine Transporter Structure-Function Studies
    CHRISTOPHER SURRATT; Fiscal Year: 2007
    ..competing DAT or SERT ligands, and assessment of cell surface expression of the transporter proteins via immunoblotting techniques...
  70. FLUORESCENT AND LARGE METAL CLUSTER COMBINATION PROBES
    Richard Powell; Fiscal Year: 1999
    ..In the initial phase, anti-IgG antibody-conjugated probes would be evaluated by immunoblotting serially diluted IgG (with silver enhancement) and as secondary reagents for immunofluorescence labeling of ..
  71. DEVELOPMENTAL CHANGES IN HEART AND MUSCLE PFK ISOZYMES
    George Dunaway; Fiscal Year: 1991
    ..subunits which are present in the native PFK isozyme pools employing SDS-polyacrylamide gel electrophoresis, immunoblotting or silver staining and quantitative gel scanning...
  72. Immune Recognition of Modified Antigen in Scleroderma
    Robert Hoffman; Fiscal Year: 2003
    ..known to have anti-70k antibodies from our large well-characterized cohort of patients using oxidative 70k and immunoblotting. Specificity will be determined in blocking studies using molar excess of either intact, apoptotic 70k or ..
  73. REGULATION OF LDL RECEPTORS IN ADIPOCYTES
    FREDRIC KRAEMER; Fiscal Year: 1993
    ..receptor content in subcellular fractions isolated by differential centrifugation will be quantitated using immunoblotting with specific anti-LDL receptor antibodies, LDL receptors in subcellular fractions will be quantitated using ..
  74. DIRECT NEUROTOXICITY OF LOCAL ANESTHETICS
    Michael Johnson; Fiscal Year: 2002
    ..b>Immunoblotting and fluorogenic assays of fractionated cell lysates will also be utilized...
  75. Biochemical Basis of Cortisone Reductase Deficiency
    Perrin White; Fiscal Year: 2007
    ..and human tissues by measuring mRNA levels using quantitative PCR, and by measuring protein expression using immunoblotting and assays of enzymatic activity...