immunoassay

Summary

Summary: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.

Top Publications

  1. ncbi Identifying early markers of Alzheimer's disease using quantitative multiplex proteomic immunoassay panels
    Holly D Soares
    Pfizer Global Research and Development, Groton, Connecticut, USA
    Ann N Y Acad Sci 1180:56-67. 2009
  2. pmc Systematic review: T-cell-based assays for the diagnosis of latent tuberculosis infection: an update
    Madhukar Pai
    McGill University and Montreal Chest Institute, Montreal, Quebec, Canada
    Ann Intern Med 149:177-84. 2008
  3. ncbi Assay for the measurement of copeptin, a stable peptide derived from the precursor of vasopressin
    Nils G Morgenthaler
    Research Department, B R A H M S AG, Biotechnology Centre Hennigsdorf Berlin, Germany
    Clin Chem 52:112-9. 2006
  4. pmc Accuracy of urine circulating cathodic antigen (CCA) test for Schistosoma mansoni diagnosis in different settings of Côte d'Ivoire
    Jean T Coulibaly
    Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland
    PLoS Negl Trop Dis 5:e1384. 2011
  5. ncbi Comparison of serum testosterone and estradiol measurements in 3174 European men using platform immunoassay and mass spectrometry; relevance for the diagnostics in aging men
    Ilpo T Huhtaniemi
    Department of Surgery and Cancer, Imperial College London, Hammersmith Campus, London, UK
    Eur J Endocrinol 166:983-91. 2012
  6. ncbi Specific and sensitive diagnosis of schistosome infection: can it be done with antibodies?
    Michael J Doenhoff
    School of Biological Sciences, University of Wales, Bangor, Gwynedd, Wales, UK LL57 2UW
    Trends Parasitol 20:35-9. 2004
  7. ncbi TR-FRET-based duplex immunoassay reveals an inverse correlation of soluble and aggregated mutant huntingtin in huntington's disease
    Barbara Baldo
    Neuroscience Discovery, Novartis Institutes for BioMedical Research, Basel CH 4002, Switzerland
    Chem Biol 19:264-75. 2012
  8. pmc A rapid, noninvasive immunoassay for frataxin: utility in assessment of Friedreich ataxia
    Eric C Deutsch
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mol Genet Metab 101:238-45. 2010
  9. ncbi Cytometric bead array: a multiplexed assay platform with applications in various areas of biology
    Edward Morgan
    BD Biosciences, San Diego, CA 92121, USA
    Clin Immunol 110:252-66. 2004
  10. ncbi Serum insulin-like growth factor I reference values for an automated chemiluminescence immunoassay system: results from a multicenter study
    Georg Brabant
    Department of Gastroenterology, Hepatology and Endocrinology, Medizinische Hochschule, Hannover, Germany
    Horm Res 60:53-60. 2003

Detail Information

Publications395 found, 100 shown here

  1. ncbi Identifying early markers of Alzheimer's disease using quantitative multiplex proteomic immunoassay panels
    Holly D Soares
    Pfizer Global Research and Development, Groton, Connecticut, USA
    Ann N Y Acad Sci 1180:56-67. 2009
    ..Multivariate analysis of an 89-analyte multivariate panel yielded a diagnostic accuracy of 70% suggesting a plasma-based AD signature that may be a useful screening tool...
  2. pmc Systematic review: T-cell-based assays for the diagnosis of latent tuberculosis infection: an update
    Madhukar Pai
    McGill University and Montreal Chest Institute, Montreal, Quebec, Canada
    Ann Intern Med 149:177-84. 2008
    ..A recent meta-analysis showed that IGRAs have high specificity, even among populations that have received bacille Calmette-Guérin (BCG) vaccination. Sensitivity was suboptimal for TST and IGRAs...
  3. ncbi Assay for the measurement of copeptin, a stable peptide derived from the precursor of vasopressin
    Nils G Morgenthaler
    Research Department, B R A H M S AG, Biotechnology Centre Hennigsdorf Berlin, Germany
    Clin Chem 52:112-9. 2006
    ..We present a method for quantifying AVP release by use of copeptin, a glycopeptide comprising the C-terminal part of the AVP prohormone...
  4. pmc Accuracy of urine circulating cathodic antigen (CCA) test for Schistosoma mansoni diagnosis in different settings of Côte d'Ivoire
    Jean T Coulibaly
    Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland
    PLoS Negl Trop Dis 5:e1384. 2011
    ..We assessed the accuracy of a commercially available CCA cassette test (designated CCA-A) and an experimental formulation (CCA-B) for S. mansoni diagnosis...
  5. ncbi Comparison of serum testosterone and estradiol measurements in 3174 European men using platform immunoassay and mass spectrometry; relevance for the diagnostics in aging men
    Ilpo T Huhtaniemi
    Department of Surgery and Cancer, Imperial College London, Hammersmith Campus, London, UK
    Eur J Endocrinol 166:983-91. 2012
    ..Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce...
  6. ncbi Specific and sensitive diagnosis of schistosome infection: can it be done with antibodies?
    Michael J Doenhoff
    School of Biological Sciences, University of Wales, Bangor, Gwynedd, Wales, UK LL57 2UW
    Trends Parasitol 20:35-9. 2004
  7. ncbi TR-FRET-based duplex immunoassay reveals an inverse correlation of soluble and aggregated mutant huntingtin in huntington's disease
    Barbara Baldo
    Neuroscience Discovery, Novartis Institutes for BioMedical Research, Basel CH 4002, Switzerland
    Chem Biol 19:264-75. 2012
    ..We developed a one-step TR-FRET-based immunoassay to quantify soluble and aggregated mHtt in cell and tissue homogenates...
  8. pmc A rapid, noninvasive immunoassay for frataxin: utility in assessment of Friedreich ataxia
    Eric C Deutsch
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mol Genet Metab 101:238-45. 2010
    ..Recently, a rapid, noninvasive lateral flow immunoassay was developed to accurately measure picogram levels of frataxin protein and shown to distinguish lymphoblastoid ..
  9. ncbi Cytometric bead array: a multiplexed assay platform with applications in various areas of biology
    Edward Morgan
    BD Biosciences, San Diego, CA 92121, USA
    Clin Immunol 110:252-66. 2004
    ..The CBA technology provides the ability to quantify multiple proteins within a given sample, with precision and consistency...
  10. ncbi Serum insulin-like growth factor I reference values for an automated chemiluminescence immunoassay system: results from a multicenter study
    Georg Brabant
    Department of Gastroenterology, Hepatology and Endocrinology, Medizinische Hochschule, Hannover, Germany
    Horm Res 60:53-60. 2003
    ..The objective of the present study was to develop cross-sectional reference values for S-IGF-I measured by an automated chemiluminescence immunoassay (Nichols Advantage).
  11. ncbi Evaluation of a novel chromatographic immunoassay based on Dual-Path Platform technology (DPP® CVL rapid test) for the serodiagnosis of canine visceral leishmaniasis
    Gabriel Grimaldi
    Laboratorio de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz FIOCRUZ, Av Brasil 4365, Rio de Janeiro, RJ 21040 360, Brazil
    Trans R Soc Trop Med Hyg 106:54-9. 2012
    ....
  12. ncbi A novel immunoassay using recombinant allergens simplifies peanut allergy diagnosis
    F Codreanu
    EA3999 Maladies allergiques diagnostic et thérapeutique, Department of Internal Medicine, Clinical Immunology and Allergology, University Hospital, Nancy, France
    Int Arch Allergy Immunol 154:216-26. 2011
    ..Thus, a simpler and safer diagnosis procedure is needed. The aim of this study was to evaluate the diagnostic performance of a new set of in vitro blood tests for peanut allergy...
  13. pmc Multiplexed immunoassay panel identifies novel CSF biomarkers for Alzheimer's disease diagnosis and prognosis
    Rebecca Craig-Schapiro
    Department of Neurology, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS ONE 6:e18850. 2011
    ..We utilized a targeted proteomics approach to discover novel cerebrospinal fluid (CSF) biomarkers that can augment the diagnostic and prognostic accuracy of current leading CSF biomarkers (Aβ42, tau, p-tau181)...
  14. ncbi Suppression of non-specific binding in serological Luminex assays
    Tim Waterboer
    Infection and Cancer Program, German Cancer Research Center DKFZ, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany
    J Immunol Methods 309:200-4. 2006
    ....
  15. pmc Simultaneous quantitation of antibodies to neutralizing epitopes on virus-like particles for human papillomavirus types 6, 11, 16, and 18 by a multiplexed luminex assay
    David Opalka
    Virus and Cell Biology, Merck Research Laboratories, Wayne, Pennsylvania 19087 8630, USA
    Clin Diagn Lab Immunol 10:108-15. 2003
    ..The HPV-Luminex competitive immunoassay measures titers of polyclonal antibodies in serum capable of displacing phycoerythrin-labeled detection ..
  16. pmc Multisite comparison of high-sensitivity multiplex cytokine assays
    Elizabeth Crabb Breen
    UCLA Cousins Center for Psychoneuroimmunology, Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 7076, USA
    Clin Vaccine Immunol 18:1229-42. 2011
    ....
  17. ncbi An evaluation of urine-CCA strip test and fingerprick blood SEA-ELISA for detection of urinary schistosomiasis in schoolchildren in Zanzibar
    J Russell Stothard
    Wolfson Wellcome Biomedical Laboratories, Department of Zoology, Natural History Museum, London, UK
    Acta Trop 111:64-70. 2009
    ..The SEA-ELISA holds promise as a complementary field-based method for monitoring infection dynamics in schoolchildren over and above standard parasitological methods...
  18. pmc Global sequence variation in the histidine-rich proteins 2 and 3 of Plasmodium falciparum: implications for the performance of malaria rapid diagnostic tests
    Joanne Baker
    Department of Drug Resistance and Diagnostics, Australian Army Malaria Institute, Brisbane, Australia
    Malar J 9:129. 2010
    ..This is of particular concern for Plasmodium falciparum histidine-rich protein 2 (PfHRP2)-detecting RDTs since PfHRP2 has been reported to be highly variable in isolates of the Asia-Pacific region...
  19. ncbi Development of a rapid, simple dipstick dye immunoassay for schistosomiasis diagnosis
    Yinchang Zhu
    Jiangsu Institute of Parasitic Diseases, Jiangsu 214064, Wuxi, PR China
    J Immunol Methods 266:1-5. 2002
    ..The results showed that the sensitivity of the dipstick dye immunoassay (DDIA) was 96.7% in 30 cases of acute schistosomiasis (29/30) and 94...
  20. ncbi Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey
    Geertruida A Posthuma-Trumpie
    Department of Psychiatry, University Medical Centre Groningen, University of Groningen, Hanzeplein 1, P O Box 30 001, 9700 RB, Groningen, The Netherlands
    Anal Bioanal Chem 393:569-82. 2009
    ..Articles referred to in this survey were searched for on MEDLINE, Scopus and in references of reviewed papers. Search terms included "immunochromatography", "sol particle immunoassay", "lateral flow immunoassay" and "dipstick assay".
  21. ncbi Enhanced electrochemiluminescence of CdSe quantum dots composited with CNTs and PDDA for sensitive immunoassay
    Guifen Jie
    Key Laboratory of Analytical Chemistry for Life Science Ministry of Education of China, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China
    Biosens Bioelectron 24:3352-8. 2009
    ..This work opened the new avenues for applying QDs ECL in highly sensitive bioassays...
  22. pmc Evaluation of a newly developed lateral flow immunoassay for the diagnosis of cryptococcosis
    Mark D Lindsley
    Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Clin Infect Dis 53:321-5. 2011
    ..This study compares the performance of a recently developed lateral flow immunoassay (LFA) to blood culture and enzyme immunoassay (EIA) for the diagnosis of cryptococcosis.
  23. ncbi Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry
    Dmitry R Bandura
    Department of Chemistry, University of Toronto, 80 St George Street, Toronto, Ontario M5S 3H6, Canada
    Anal Chem 81:6813-22. 2009
    ..The mass range (m/z = 125-215) and abundance sensitivity are sufficient for elemental immunoassay with up to 60 distinct available elemental tags...
  24. pmc Elimination of false-positive results in a luminex assay for pneumococcal antibodies
    Jerry W Pickering
    ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    Clin Vaccine Immunol 17:185-9. 2010
    ..The first group bound nonspecifically to the Luminex microspheres. The second group reacted specifically with bovine serum albumin (BSA). We describe here methods that eliminated the false-positive reactivity of both groups...
  25. pmc Prerequisites for cytokine measurements in clinical trials with multiplex immunoassays
    Wilco de Jager
    Department of Pediatric Immunology, Centre for Molecular and Cellular Intervention CMCI, University Medical Centre Utrecht, Utrecht, The Netherlands
    BMC Immunol 10:52. 2009
    ..Therefore we set out to investigate several processes that might impact cytokine profiles such as blood collecting tubes, duration of storage, and number of freeze thawing cycles...
  26. ncbi Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay
    Kai Grossmann
    Lausitz University of Applied Sciences, Senftenberg, Germany
    Cytometry A 79:118-25. 2011
    ..detection system VideoScan (AKLIDES®) was used for the fluorescence analysis of a multiplex microbead-based immunoassay (MIA)...
  27. pmc Standardization of misleading immunoassay based 25-hydroxyvitamin D levels with liquid chromatography tandem-mass spectrometry in a large cohort study
    Ben Schöttker
    Division of Clinical Epidemiology and Aging Research, German Cancer Research Center, Heidelberg, Germany
    PLoS ONE 7:e48774. 2012
    ..We demonstrate problems in comparability of measures by different immunoassays and the need for standardization in the context of a large population-based cohort study...
  28. ncbi Microarrayed recombinant allergens for diagnostic testing
    Wayne G Shreffler
    Food Allergy Center and Center for Immunology and Inflammatory Diseases at Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    J Allergy Clin Immunol 127:843-9; quiz 850-1. 2011
    ..Promising findings, particularly in the context of food and latex allergy, point to the potential for greater resolution between clinical reactivity and asymptomatic sensitization with this platform...
  29. ncbi Selected reaction monitoring-mass spectrometric immunoassay responsive to parathyroid hormone and related variants
    Mary F Lopez
    ThermoFisher Scientific BRIMS, Cambridge, MA, USA
    Clin Chem 56:281-90. 2010
    ....
  30. pmc Sensitive immunosensor for cancer biomarker based on dual signal amplification strategy of graphene sheets and multienzyme functionalized carbon nanospheres
    Dan Du
    Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    Anal Chem 82:2989-95. 2010
    ..02 ng mL(-1) AFP with a linear calibration range from 0.05 to 6 ng mL(-1). This amplification strategy is a promising platform for clinical screening of cancer biomarkers and point-of-care diagnostics...
  31. ncbi A novel sensitive immunoassay by nucleic acid barcode dot and its application in the detection of prostate-specific antigen
    Xiao Li Kong
    Clinical Medical Research Center, The 2nd Clinic Medicine College Shenzhen People s Hospital, Jinan University, Shenzhen, PR China
    Clin Chem Lab Med 48:279-83. 2010
    ..It also requires specialized equipment, which limits its application. We introduce a nano-nucleic acid barcode dot detection technology to determine ultramicro concentrations of protein. The method is simple, quick and accurate...
  32. pmc Comparison of two dengue NS1 rapid tests for sensitivity, specificity and relationship to viraemia and antibody responses
    Vianney Tricou
    Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, District 5, Ho Chi Minh City, Viet Nam
    BMC Infect Dis 10:142. 2010
    ..The detection of DENV NS1 on rapid lateral flow tests offers a fast route to a presumptive dengue diagnosis but careful evaluations are urgently needed as more and more people use them...
  33. pmc Performance of a dipstick dye immunoassay for rapid screening of Schistosoma japonicum infection in areas of low endemicity
    Jing Xu
    National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, People s Republic of China
    Parasit Vectors 4:87. 2011
    The dipstick dye immunoassay (DDIA), recently commercially available in the People's Republic of China (P.R. China), is a rapid and simple test to detect human antibodies against Schistosoma Japonicum...
  34. ncbi A highly sensitive electrochemiluminescence immunoassay for the neurofilament heavy chain protein
    Jens Kuhle
    Clinical Neuroimmunology, Neurology and Department of Biomedicine, University Hospital Basel, Switzerland
    J Neuroimmunol 220:114-9. 2010
    ..Electrochemiluminescence (ECL) based assays are known to be of superior sensitivity and require less sample volume than conventional ELISAs...
  35. pmc Evaluation of immunoassays for the diagnosis of Schistosoma japonicum infection using archived sera
    Jing Xu
    National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, People s Republic of China
    PLoS Negl Trop Dis 5:e949. 2011
    ..We compared the performance of nine immunodiagnostic tests developed in P.R. China for detection of antibodies against S. japonicum and established their priority for further assessment in field settings...
  36. ncbi Solid-phase and bead-based cytokine immunoassay: a comparison
    Wilco de Jager
    Department of Pediatric Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
    Methods 38:294-303. 2006
    ..sandwich immunoassays such as enzyme-linked immunosorbent assay (ELISA) or with a bead based multiplex immunoassay (MIA)...
  37. pmc Current and developing technologies for monitoring agents of bioterrorism and biowarfare
    Daniel V Lim
    Department of Biology, Center for Biological Defense, University of South Florida, Tampa, FL 33620 5200, USA
    Clin Microbiol Rev 18:583-607. 2005
    ..Although there is no ideal platform, many of these technologies have proved invaluable for the detection and identification of biothreat agents...
  38. pmc Evaluation of a rapid diagnostic test (CareStart Malaria HRP-2/pLDH (Pf/pan) Combo Test) for the diagnosis of malaria in a reference setting
    Jessica Maltha
    Department of Medical Microbiology, Faculty of Health, Medicine and Life Sciences FHML, Maastricht, The Netherlands
    Malar J 9:171. 2010
    ....
  39. pmc Validation of a microsphere-based immunoassay for detection of anti-West Nile virus and anti-St. Louis encephalitis virus immunoglobulin m antibodies
    Alison J Johnson
    Division of Vector Borne Infectious Diseases, National Center for Zoonotic, Vector Borne and Enteric Diseases, Centers for Disease Control and Prevention, 3150 Rampart Rd, Foothills Campus, Fort Collins, CO 80521, USA
    Clin Vaccine Immunol 14:1084-93. 2007
    A microsphere-based immunoassay (MIA) was previously developed that is capable of determining the presence of anti-West Nile (WN) virus or anti-St...
  40. ncbi Development and validation of a multiplex immunoassay for the simultaneous determination of serum antibodies to Bordetella pertussis, diphtheria and tetanus
    Pieter G M van Gageldonk
    Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
    J Immunol Methods 335:79-89. 2008
    ..The method was validated with different serum panels (n=60-78 samples). With the Multiplex Immunoassay (MIA) no evidence for bead interference between monoplex and pentaplex was found...
  41. pmc Comparison of multiplex immunoassay platforms
    Qin Fu
    Departments of Medicine, Biomedical Engineering, Biological Chemistry, Bayview Proteomics Center, Johns Hopkins University, Baltimore, MD 21224, USA
    Clin Chem 56:314-8. 2010
    ..We have measured the reproducibility, reliability, robustness, accuracy, and throughput of commercially available multiplex immunoassays to ascertain their suitability for serum biomarker analysis and validation...
  42. pmc Antibody-based protein multiplex platforms: technical and operational challenges
    Allison A Ellington
    Division of Cardiovascular Diseases, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    Clin Chem 56:186-93. 2010
    ..This concept has stimulated development of multiplexed immunoassay platforms that provide multiple, parallel protein measurements on the same specimen.
  43. ncbi A one-step homogeneous immunoassay for cancer biomarker detection using gold nanoparticle probes coupled with dynamic light scattering
    Xiong Liu
    NanoScience Technology Center, Department of Chemistry, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, Florida 32826, USA
    J Am Chem Soc 130:2780-2. 2008
    A one-step homogeneous immunoassay for the detection of a prostate cancer biomarker, free-PSA (prostate specific antigen), was developed using gold nanoparticle probes coupled with dynamic light scattering (DLS) measurements...
  44. ncbi Analytical error and interference in immunoassay: minimizing risk
    Catharine M Sturgeon
    Department of Clinical Biochemistry, Royal Infirmary of Edinburgh, 51 Little France Crescent, Edinburgh EH16 4SA, UK
    Ann Clin Biochem 48:418-32. 2011
    ....
  45. ncbi Automated immunoassay system for AFP-L3% using on-chip electrokinetic reaction and separation by affinity electrophoresis
    Chiaki Kagebayashi
    Osaka Research Laboratory, New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries Ltd, 6 1 Takada cho, Amagasaki, Hyogo Prefecture, Japan
    Anal Biochem 388:306-11. 2009
    Implementation of the on-chip immunoassay for alpha-fetoprotein (AFP)-L3% was achieved using a fully automated microfluidic instrument platform that will prepare the chip and run the assay with a total assay time of less than 10min...
  46. ncbi Sensitivity and specificity of a point-of-care matrix metalloproteinase 9 immunoassay for diagnosing inflammation related to dry eye
    Robert Sambursky
    Manatee Sarasota Eye Clinic and Laser Center, Sarasota, Bradenton, FL 34202, USA
    JAMA Ophthalmol 131:24-8. 2013
    ..To determine the clinical sensitivity, specificity, negative predictive value, and positive predictive value of a rapid point-of-care diagnostic test to detect elevated matrix metalloproteinase 9 levels (InflammaDry)...
  47. pmc Rapid detection of Mycobacterium tuberculosis biomarkers in a sandwich immunoassay format using a waveguide-based optical biosensor
    Harshini Mukundan
    Chemistry Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Tuberculosis (Edinb) 92:407-16. 2012
    ..Currently, traditional immunoassay platforms that suffer from poor sensitivity and high non-specific interactions are used for the detection of ..
  48. ncbi Development of a sensitive detection method of cancer biomarkers in human serum (75%) using a quartz crystal microbalance sensor and nanoparticles amplification system
    Yildiz Uludag
    Cranfield University, Cranfield Health, Cranfield, Bedfordshire MK43 0AL, England, UK
    Talanta 82:277-82. 2010
    ..With the achieved detection limit in serum samples, the developed QCM assay shows a promising technology for cancer biomarker analysis in patient samples...
  49. ncbi Development of a multiplex microsphere immunoassay for the quantitation of salivary antibody responses to selected waterborne pathogens
    Shannon M Griffin
    Oak Ridge Institute for Science and Education, Oak Ridge, TN, USA
    J Immunol Methods 364:83-93. 2011
    ..The development of a Luminex multiplex immunoassay for measurement of salivary IgG and IgA responses to potentially waterborne pathogens, Helicobacter pylori, ..
  50. ncbi Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay
    Sandeep Kumar Vashist
    Centre for Bioanalytical Sciences CBAS, National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland
    Analyst 136:4431-6. 2011
    ..and combinations of each) were examined to determine their performance in a surface plasmon resonance-based immunoassay using human fetuin A (HFA) as the model antigen system...
  51. pmc Bio-Rad's Bio-Plex® suspension array system, xMAP technology overview
    Brett Houser
    Bio Rad Laboratories, Inc, Hercules, California 94547, USA
    Arch Physiol Biochem 118:192-6. 2012
    ..at analytes simultaneously providing more information from less sample volume in less time than traditional immunoassay methods...
  52. ncbi Upconversion fluorescence resonance energy transfer in a homogeneous immunoassay for estradiol
    Katri Kuningas
    Department of Biotechnology, University of Turku, Tykistokatu 6A, FIN 20520 Turku, Finland
    Anal Chem 78:4690-6. 2006
    ..The UC-FRET has now been applied to a competitive homogeneous immunoassay for 17beta-estradiol (E2) in serum, using a small-molecular dye as an acceptor...
  53. pmc Malaria diagnostics in clinical trials
    Sean C Murphy
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland Yale University School of Public Health, New Haven, Connecticut Office of HIV AIDS Network Coordination, Fred Hutchinson Cancer Research Center, Seattle, Washington Hydas World Health, Hershey, Pennsylvania Uniformed Services University of the Health Sciences, Bethesda, Maryland
    Am J Trop Med Hyg 89:824-39. 2013
    ..Performance evaluation, quality control, and external quality assessment are critical processes that must be implemented in all clinical trials using malaria tests...
  54. ncbi Electrochemical immunosensor for ultrasensitive detection of microcystin-LR based on graphene-gold nanocomposite/functional conducting polymer/gold nanoparticle/ionic liquid composite film with electrodeposition
    Li Ruiyi
    National University of Ireland, Maynooth, Co Kildare, Ireland
    Biosens Bioelectron 44:235-40. 2013
    ..It has been successfully applied to the microcystin-LR determination in water samples with a spiked recovery in the range of 96.3-105.8%...
  55. ncbi Portable optical immunosensor for highly sensitive detection of microcystin-LR in water samples
    F Long
    Environmental Simulate and Pollution Control State Key Joint Laboratory, Dept of Environment Science and Engineering, Tsinghua University, Beijing 100084, China
    Biosens Bioelectron 24:2346-51. 2009
    ..MC-LR) was conducted with a portable trace organic pollutant analyzer (TOPA) based on the principle of immunoassay and total internal reflection fluorescence...
  56. ncbi Compact quantitative optic fiber-based immunoarray biosensor for rapid detection of small analytes
    Feng Long
    Environmental Simulation and Pollution Control State Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing, 100084, China
    Biosens Bioelectron 26:16-22. 2010
    ..This compact and portable quantitative immunoarray provides an excellent multiple assay platform for clinical and environmental samples...
  57. ncbi Performance of an organic photodiode as an optical detector and its application to fluorometric flow-immunoassay for IgA
    Mayo Miyake
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi ku, Fukuoka 819 0395, Japan
    Talanta 96:132-9. 2012
    ..1μW/cm(2). The OPD was successfully used in a flow-immunoassay for IgA, a marker of human stress, in which a sandwich immunoassay was carried out on the microchip and the ..
  58. pmc Detection of immune-complex-dissociated nonstructural-1 antigen in patients with acute dengue virus infections
    Penelopie Koraka
    Laboratory for Exotic Viral Infections, Institute of Virology, Erasmus MC, Rotterdam, The Netherlands
    J Clin Microbiol 41:4154-9. 2003
    ..the diagnostic value of a newly developed immune-complex dissociated nonstructural-1 (NS-1) antigen dot blot immunoassay (DBI) was compared to a commercially available DEN antigen detection kit (denKEY Blue kit; Globio Co...
  59. ncbi Simultaneous determination of HIV antibodies, hepatitis C antibodies, and hepatitis B antigens in dried blood spots--a feasibility study using a multi-analyte immunoassay
    Zoltan Lukacs
    Department of Pediatrics, Metabolic Laboratory, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Clin Chem Lab Med 43:141-5. 2005
    ..We demonstrated the feasibility of the simultaneous detection of infectious diseases from dried blood. Our novel method also provides a convenient tool for monitoring children from HIV-positive mothers and for possible screening efforts...
  60. ncbi Harmonization of immune biomarker assays for clinical studies
    Sjoerd H van der Burg
    Department of Clinical Oncology, Leiden University Medical Center, Leiden, Netherlands
    Sci Transl Med 3:108ps44. 2011
    ..This process enables objective interpretation and comparison of data across clinical trial sites and also facilitates the identification of relevant immune biomarkers, guiding the development of new therapies...
  61. ncbi Early diagnosis of acute myocardial infarction in the elderly using more sensitive cardiac troponin assays
    Miriam Reiter
    Department of Internal Medicine, University Hospital, Petersgraben 4, CH 4031 Basel, Switzerland
    Eur Heart J 32:1379-89. 2011
    ..To examine the diagnostic accuracy of sensitive cardiac troponin (cTn) assays in elderly patients, since elevated levels with sensitive cTn assays were reported in 20% of elderly patients without acute myocardial infarction (AMI)...
  62. ncbi Comparison of techniques to screen and characterize bacteria-specific hybridomas for high-quality monoclonal antibodies selection
    Ratthaphol Charlermroj
    National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Pathumthani 12120, Thailand
    Anal Biochem 421:26-36. 2012
    ..While the antibody array was slightly less sensitive than ELISA, a much faster and lower cost procedure to screen clones against multiple antigens has been demonstrated...
  63. pmc Lateral-flow immunoassay for the frataxin protein in Friedreich's ataxia patients and carriers
    John H Willis
    MitoSciences, Inc, Research and Development, 1850 Millrace Drive, Suite 3A, Eugene, OR 97403, USA
    Mol Genet Metab 94:491-7. 2008
    ..We designed a lateral-flow immunoassay that quantifies frataxin protein levels in a variety of sample materials...
  64. ncbi Evaluation of three rapid screening assays for detection of antibodies to hepatitis C virus
    Bryce D Smith
    Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Infect Dis 204:825-31. 2011
    ..Although therapies are available that can suppress or eliminate infection, identifying persons infected with HCV is challenging. Rapid tests could help identify many of these persons more expeditiously...
  65. ncbi Measurement of 25-hydroxyvitamin D in the clinical laboratory: current procedures, performance characteristics and limitations
    A M Wallace
    Department of Clinical Biochemistry, Macewen Building, Royal Infirmary, Glasgow G4 0SF, UK
    Steroids 75:477-88. 2010
    ..The two main types of methods are competitive immunoassay and those based on chromatographic separation followed by non-immunological direct detection (HPLC, LC-MS/MS)...
  66. ncbi State-of-the-art vitamin D assays: a comparison of automated immunoassays with liquid chromatography-tandem mass spectrometry methods
    Christopher John L Farrell
    Laverty Pathology, North Ryde, New South Wales, Australia
    Clin Chem 58:531-42. 2012
    ..Furthermore, preexisting assays from DiaSorin and Roche have recently been modified. We compared the performance of 5 automated immunoassays, an RIA and 2 liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods...
  67. pmc Peptide microarray analysis of in silico-predicted epitopes for serological diagnosis of Toxoplasma gondii infection in humans
    Pavlo Maksimov
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Wusterhausen, Germany
    Clin Vaccine Immunol 19:865-74. 2012
    ..gondii epitopes as candidate antigens for serological diagnosis...
  68. ncbi Evaluation of a new and automated electrochemiluminescence immunoassay for plasma sFlt-1 and PlGF levels in women with preeclampsia
    Akihide Ohkuchi
    Department of Obstetrics and Gynecology, Jichi Medical University School of Medicine, Shimotsuke shi, Tochigi, Japan
    Hypertens Res 33:422-7. 2010
    ..In conclusion, measurement of the plasma sFlt-1/PlGF ratio determined by Elecsys sFlt-1 and Elecsys PlGF and using a cutoff value of 45 might assist in the diagnosis of preeclampsia, especially for early-onset preeclampsia...
  69. ncbi Highly sensitive, automated immunoassay for immunoglobulin free light chains in serum and urine
    A R Bradwell
    Department of Immunology, The Medical School, Edgbaston, Birmingham B15 2TT, United Kingdom
    Clin Chem 47:673-80. 2001
    ..Automated immunoassays that measure FLCs in urine and serum have considerable clinical potential...
  70. ncbi The biomimetic immunoassay based on molecularly imprinted polymer: a comprehensive review of recent progress and future prospects
    Z X Xu
    College of Food Science and Engineering, Shandong Agricultural Univ, Taian 271018, China
    J Food Sci 76:R69-75. 2011
    b>Immunoassay, based on a selective affinity of the biological antibody for its antigen, is one of the most usual analytical methods in food safety and environmental chemistry...
  71. pmc Opportunities for improving pLDH-based malaria diagnostic tests
    Robert C Piper
    Flow Incorporated, 6127 SW Corbett, Portland, OR 97239 3601, USA
    Malar J 10:213. 2011
    ....
  72. ncbi A method to solubilise protein aggregates for immunoassay quantification which overcomes the neurofilament "hook" effect
    Ching Hua Lu
    Sobell Department of Motor Neuroscience and Movement Disorders, UCL Institute of Neurology, University College London, London WC1N3BG, UK
    J Neurosci Methods 195:143-50. 2011
    ..as a potential disease biomarker, Nf aggregates may mask Nf epitopes, preventing accurate quantification by immunoassay. Using the SOD1(G93A) mouse model of amyotrophic lateral sclerosis, we developed a method to disrupt Nf ..
  73. ncbi A highly sensitive immunoassay for the detection of prion-infected material in whole human blood without the use of proteinase K
    M Howard Tattum
    MRC Prion Unit and the Department of Neurodegenerative Disease, Institute of Neurology, University College London, London, UK
    Transfusion 50:2619-27. 2010
    ....
  74. pmc Novel point-of-care test for simultaneous detection of nontreponemal and treponemal antibodies in patients with syphilis
    Arnold R Castro
    Laboratory Reference and Research Branch, Division of STD Prevention, Centers for Disease Control and Prevention, 1600 Clifton Road, MS A 12, Atlanta, GA 30333, USA
    J Clin Microbiol 48:4615-9. 2010
    ....
  75. pmc Simultaneous measurement of antigen-stimulated interleukin-1 beta and gamma interferon production enhances test sensitivity for the detection of Mycobacterium bovis infection in cattle
    Gareth J Jones
    TB Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom
    Clin Vaccine Immunol 17:1946-51. 2010
    ..In conclusion, we have demonstrated that the use of an additional readout system, such as IL-1β, can potentially complement IFN-γ by increasing overall test sensitivity for the detection of M. bovis infection in cattle...
  76. ncbi A label-free immunoassay based upon localized surface plasmon resonance of gold nanorods
    Kathryn M Mayer
    Department of Physics and Astronomy, Rice University, Houston, Texas 77005, USA
    ACS Nano 2:687-92. 2008
    ....
  77. pmc Short-term reproducibility of a commercial interferon gamma release assay
    A K Detjen
    Desmond Tutu Tuberculosis Centre, Department of Paediatrics and Child Health, Stellenbosch University, Tygerberg, South Africa
    Clin Vaccine Immunol 16:1170-5. 2009
    ..The results indicate that the QFT-IT assay is a robust and highly reproducible assay. Considerable intraindividual variability occurs in the magnitude of IFN-gamma responses, which may influence the interpretation of serial measures...
  78. pmc Comparison of CareStart™ HRP2/pLDH COMBO rapid malaria test with light microscopy in north-west Ethiopia
    Beyene Moges
    Department of Immunology and Molecular Biology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, 196, Gondar, Ethiopia
    Malar J 11:234. 2012
    ..This study was aimed at testing the diagnostic performance of CareStart™ malaria rapid diagnostic test (RDT) with reference to light microscopy for the diagnosis of falciparum and vivax malaria in Ethiopia...
  79. pmc Development of a multiplexed bead-based immunoassay for the simultaneous detection of antibodies to 17 pneumococcal proteins
    S Shoma
    International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh
    Eur J Clin Microbiol Infect Dis 30:521-6. 2011
    ..We developed a multiplex bead-based immunoassay (xMAP(®) Technology, Luminex Corporation) to simultaneously quantify antibodies against these 17 pneumococcal ..
  80. ncbi An integrated microfluidic system for rapid diagnosis of dengue virus infection
    Yu Fang Lee
    Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan
    Biosens Bioelectron 25:745-52. 2009
    ..This integrated system may provide a powerful platform for the rapid diagnosis of dengue virus infection and other types of infectious diseases...
  81. ncbi Use of an immunochromatographic kit for the rapid detection of Mycobacterium tuberculosis from broth cultures
    N A Ismail
    Department of Medical Microbiology, University of Pretoria and the Microbiology Diagnostic Laboratory, Tshwane Academic Division, National Health Laboratory Service NHLS, Pretoria, South Africa
    Int J Tuberc Lung Dis 13:1045-7. 2009
    ..The kit requires a 15-min assay time, is easy to perform and is a good method for simplifying the diagnosis of TB...
  82. ncbi Evaluation of rapid diagnostic tests for malaria case management in Gabon
    Denise Patricia Mawili-Mboumba
    Department of Parasitology Mycology and Tropical Medicine, Universite des Sciences de la Sante, Libreville, Gabon
    Diagn Microbiol Infect Dis 66:162-8. 2010
    ..These tests should be considered as a good alternative to microscopy, allowing not only an efficient and rapid diagnosis of malaria in primary health facilities but also to aid in promoting changes for antimalarial prescription behavior...
  83. pmc Multiplex immunoassay for serological diagnosis of Mycobacterium bovis infection in cattle
    Clare Whelan
    Enfer Scientific, Unit T, M7 Business Park, Newhall, Naas, County Kildare, Ireland
    Clin Vaccine Immunol 15:1834-8. 2008
    ..As reported here, we developed a multiplex chemiluminescent immunoassay that can simultaneously detect antibody activity to 25 antigens in a single well in a 96-well plate array ..
  84. ncbi Development of a biosensor microarray towards food screening, using imaging surface plasmon resonance
    Sabina Rebe Raz
    RIKILT Institute of Food Safety, Wageningen UR, P O Box 230, 6700 AE Wageningen, The Netherlands
    Biosens Bioelectron 24:552-7. 2008
    In this study we examined the possibilities of implementing direct and competitive immunoassay formats for small and large molecule detection on a microarray, using IBIS imaging surface plasmon resonance (iSPR) system...
  85. pmc Proteinase K-sensitive disease-associated ovine prion protein revealed by conformation-dependent immunoassay
    Alana M Thackray
    Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 OES, UK
    Biochem J 401:475-83. 2007
    ..We have developed a CDI (conformation-dependent immunoassay) utilizing time-resolved fluorescence to study the conformers of disease-associated PrP in natural cases of ..
  86. ncbi Quantitative immunochemical fecal occult blood testing for colorectal adenoma detection: evaluation in the target population of screening and comparison with qualitative tests
    Ulrike Haug
    German Cancer Research Center, Heidelberg, Germany
    Am J Gastroenterol 105:682-90. 2010
    ....
  87. ncbi Combining the Capilia TB assay with smear morphology for the identification of Mycobacterium tuberculosis complex
    G H Shen
    Department of Internal Medicine, Division of Respiratory and Critical Care Medicine, Taichung Veterans General Hospital, Taichung, Taiwan, Republic of China
    Int J Tuberc Lung Dis 13:371-6. 2009
    ....
  88. ncbi A polymer lab-on-a-chip for magnetic immunoassay with on-chip sampling and detection capabilities
    Jaephil Do
    Microsystems and BioMEMS Laboratory, Center for BioMEMS and Nanobiosciences, Department of Electrical and Computer Engineering and Computer Science, University of Cincinnati, Cincinnati, OH 45221, USA
    Lab Chip 8:542-9. 2008
    This paper presents a new polymer lab-on-a-chip for magnetic bead-based immunoassay with fully on-chip sampling and detection capabilities, which provides a smart platform of magnetic immunoassay-based lab-on-a-chip for point-of-care ..
  89. ncbi Development of antiricin single domain antibodies toward detection and therapeutic reagents
    George P Anderson
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue SW, Washington, DC 20375, USA
    Anal Chem 80:9604-11. 2008
    ..These results indicate that antiricin sdAb have great potential for both diagnostic and therapeutic applications...
  90. ncbi Quantification of serum 1-84 parathyroid hormone in patients with hyperparathyroidism by immunocapture in situ digestion liquid chromatography-tandem mass spectrometry
    Vivek Kumar
    Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    Clin Chem 56:306-13. 2010
    ..A methodology using immunocapture purification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) detection, along with a stable isotope-labeled internal standard, may help address these issues...
  91. pmc Rapid, high-throughput detection of PrPSc by 96-well immunoassay
    Michelle L Kramer
    Department of Medical Microbiology and Immunology, School of Medicine, Creighton University, Omaha, NE 68178, USA
    Prion 3:44-8. 2009
    ..We have developed a rapid, high-throughput 96-well immunoassay, which is specific for the detection of PrP(Sc)...
  92. ncbi Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples
    J S Abrams
    Department of Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304
    Immunol Rev 127:5-24. 1992
  93. ncbi Molecular distinction of circulating pregnancy-associated plasma protein A in myocardial infarction and pregnancy
    Qiu Ping Qin
    Department of Biotechnology, University of Turku, Turku 20520, Finland
    Clin Chem 51:75-83. 2005
    ..Recently, increased serum concentrations of PAPP-A have been found in acute coronary syndromes (ACS). The aim of this study was to investigate whether the circulating PAPP-A in ACS is the same as that in pregnancy...
  94. pmc Plasmodium falciparum histidine-rich protein 2-based immunocapture diagnostic assay for malaria: cross-reactivity with rheumatoid factors
    J Iqbal
    Department of Microbiology, Faculty of Medicine, Kuwait University, Safat 13110, Kuwait
    J Clin Microbiol 38:1184-6. 2000
    ..Our study shows that the PfHRP-2-based ICT assay gave a false-positive reaction in 26% of the patients who had rheumatoid factors, but were negative for malaria by microscopy...
  95. pmc Genetic heterogeneity of small ruminant lentiviruses involves immunodominant epitope of capsid antigen and affects sensitivity of single-strain-based immunoassay
    Elena Grego
    Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Facolta di Medicina Veterinaria, Universita di Torino, 10095 Grugliasco, Italy
    Clin Diagn Lab Immunol 9:828-32. 2002
    ..Additionally, these results support the hypothesis of cross-species transmission based on the phylogenetic analysis described above. This has implications for the control and eradication of small ruminant lentivirus infections...
  96. ncbi Using latent class analysis to estimate the test characteristics of the γ-interferon test, the single intradermal comparative tuberculin test and a multiplex immunoassay under Irish conditions
    Tracy A Clegg
    Centre for Veterinary Epidemiology and Risk Analysis, Veterinary Sciences Centre, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
    Vet Microbiol 151:68-76. 2011
    ..class models to estimate the diagnostic characteristics of existing (SICTT and γ-IFN) and new (multiplex immunoassay [Enferplex-TB]) diagnostic tests under Irish field conditions where true disease status was unknown...
  97. ncbi Evaluation of a novel kit (TF-Test) for the diagnosis of intestinal parasitic infections
    Jancarlo Ferreira Gomes
    Department of Clinical Parasitology, State University of Campinas, Sao Paulo, Brazil
    J Clin Lab Anal 18:132-8. 2004
    ..sensitive results, we designed a practical and economical kit (TF-Test) that is now commercially available (Immunoassay Com. Ind. Ltda., São Paulo, Brazil)...
  98. pmc Capacity building permitting comprehensive monitoring of a severe case of Lassa hemorrhagic fever in Sierra Leone with a positive outcome: case report
    Jessica N Grove
    Tulane University Department of Microbiology and Immunology, New Orleans, LA, USA
    Virol J 8:314. 2011
    ..These studies provide new insights into the pathophysiology and management of this viral illness and outline the improved infrastructure, research and real-time diagnostic capabilities within LASV endemic areas...
  99. ncbi Measurement of midregional proadrenomedullin in plasma with an immunoluminometric assay
    Nils G Morgenthaler
    Research Department, B R A H M S AG, Biotechnology Centre Hennigsdorf Berlin, Germany
    Clin Chem 51:1823-9. 2005
    ..Here we report the technical evaluation of an assay for midregional pro-ADM (MR-proADM) that does not have these problems...
  100. ncbi Three biomarkers identified from serum proteomic analysis for the detection of early stage ovarian cancer
    Zhen Zhang
    Department of Pathology, Biomarker Discovery Center, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 64:5882-90. 2004
    ..After protein identification, biomarkers for which an immunoassay was available were tested on samples from the fifth center, which included 41 healthy women, 41 patients with ..
  101. pmc Malaria rapid diagnostic tests: Plasmodium falciparum infections with high parasite densities may generate false positive Plasmodium vivax pLDH lines
    Jessica Maltha
    Faculty of Health, Medicine and Life Sciences FHML, Maastricht, The Netherlands
    Malar J 9:198. 2010
    ..Previous observations of false positive Pv-pLDH test lines in P. falciparum samples incited to the present study, which assessed P. vivax-specific RDTs for the occurrence of false positive Pv-pLDH lines in P. falciparum samples...

Research Grants78

  1. Rapid Test for Monitoring Dust Mite Avoidance in Homes
    Martin Chapman; Fiscal Year: 2004
    ..The test detected mite allergen in dust samples within 10 minutes, correlated with enzyme immunoassay (ELISA) results, and was used to monitor allergen levels in homes and changes in allergen levels following an ..
  2. Sensitive Diagnosis of Biowarfare Agents on a Microchip
    Greg Collins; Fiscal Year: 2006
    ..Most importantly, the proposed microchip device will be capable of performing an entire immunoassay on several pathogens of interest, simultaneously and in a single step on the microchip...
  3. Personalized Clinical Diagnostics and Beyond: Integrated Ring Resonator Arrays
    Ryan Bailey; Fiscal Year: 2009
    ..This technology promises to broadly impact the landscape of the biomedical sciences, both meeting the clinical diagnostic challenges of today and pioneering the paradigm-shifting discoveries of tomorrow. ..
  4. Integrated Microfluidic Platform for Point-of-Care Serodiagnostics
    Xiaowu Liang; Fiscal Year: 2013
    ..The diagnosis platform is based on the well-established clinical diagnostic tool, the enzyme immunoassay (eg ELISA)...
  5. Immune Mechanism of Rejection in Human Lung Allografts
    Thalachallour Mohanakumar; Fiscal Year: 2013
    ..using ELISPOT), 5) decreasing de novo development of Abs to mismatched donor HLA (measured by solid phase immunoassay), and 6) decreasing the incidence and severity of BOS (determined by ISHLT criterion)...
  6. Salivary-based Bone-loss Marker Detection Platform for Point-of-Care Screening
    MANAL BESHAY; Fiscal Year: 2012
    ..Current laboratory-based tests such as enzyme linked immunoassay (ELISA) kits for the detection of individual biomarkers in either blood or urine are generally not offered for ..
  7. Innate Immunity, Biomarkers and Myocardial Infarction
    Gregory L Stahl; Fiscal Year: 2010
    ..We developed a novel four point immunoassay to quantify the complement components within the MBL pathway so we can screen human sera repositories and ..
  8. Novel High Affinity and Long-Acting Bovine FSH Analogs for Veterinary Superovulat
    BRUCE DALE WEINTRAUB; Fiscal Year: 2012
    ..analogs expressed by transient transfection and characterize in vitro by previously validated bFSH analog ELISA immunoassay, robust in vitro bioassay, SDS-PAGE electrophoresis and isoelectric focusing gel analysis. 3...
  9. Respiratory Panel Flow-Strip: a Point-of-Care Molecular Diagnostic Test
    CHRISTOPHER GERARD COONEY; Fiscal Year: 2012
    ..g., one study found the sensitivity of a laterl flow immunoassay test for flu to be as low as 10.4%)...
  10. Target discovery and immunoassay for diagnosis of invasive aspergillosis
    Thomas R Kozel; Fiscal Year: 2013
    ..Early diagnosis can markedly improve survival. The goal of this project is development of an immunoassay for detection of Aspergillus antigens in blood or urine to facilitate early diagnosis of IA...
  11. Urine biomarkers of lupus nephritis pathology and response to therapy
    James C Oates; Fiscal Year: 2013
    ..In the third Aim, an immunoassay for reactive-intermediate-modified Tyr will be developed, and all biomarker immunoassays will undergo method ..
  12. Lymph Node Alterations in Cancer
    M ALANNA RUDDELL; Fiscal Year: 2013
    ..immune cells could drive B cell accumulation in TDLNs, and these will be screened for by cDNA microarray or immunoassay comparison of tumors, TDLNs, and normal LNs...
  13. The Effects of Estradiol on Genetic Risk for Disordered Eating During Puberty
    Kelly L Klump; Fiscal Year: 2013
    ..Salivary and serum samples will be collected and assayed for hormone levels using standard enzyme immunoassay techniques...
  14. Nanoscale analysis of proteomic response to novel targeted therapies for lymphoma
    ALICE CHEN FAN; Fiscal Year: 2013
    ..At the bench, I have developed a novel nanofluidic proteomic immunoassay (NIA) for the analysis of oncoproteins in cells from patient specimens collected using minimally invasive ..
  15. Development of Superactive Analogs of FSH for Human Infertility
    BRUCE DALE WEINTRAUB; Fiscal Year: 2013
    ..Using an HPLC-validated immunoassay we have discovered superior pharmacokinetic properties of TR4401 in comparison to standard FSH both in rodents ..
  16. Development of Chemical Chaperonin for Medium Chain Acyl-CoA Dehydrogenase Defici
    AL WALID ABDEL MOHSEN; Fiscal Year: 2010
    ..using two random in vitro chemical library screening techniques, an HCS (high content-image based screening) immunoassay and HTS (high throughput screening) enzymatic assay, and to identify candidate compounds using in silico small ..
  17. Gram-Negative Pneumonia and Lipopolysaccharide Binding Protein
    MARK RICHARD HEMMILA; Fiscal Year: 2010
    ..In addition the research will focus on proteomics with use of a novel protein microarray immunoassay and development of LBP-like peptides...
  18. Technology to improve the assessment of tissue-associated protein biomarkers
    JEFFREY THOMAS MASON; Fiscal Year: 2013
    ..use of an innovative pressure cycling technology to solubilize tissue proteins and a novel and highly sensitive immunoassay format, called immunoliposome-polymerase chain reaction (ILPCR), developed in our laboratory will achieve the ..
  19. Rapid Point-of-Care Test Kit for the Detection of Bacteriuria Calibrated for Imme
    MARK GEISBERG; Fiscal Year: 2010
    ..The proposed test kit will be a lateral flow immunoassay, similar to a home pregnancy test...
  20. Nanoscale proteomic profiles of hypoxia pathways to develop biomarkers of renal c
    JOHN THOMAS LEPPERT; Fiscal Year: 2013
    ..We developed nanoscale immunoassay (NIA) as a highly sensitive, quantitative and unique way to concurrently measure proteins, their different ..
  21. Non-invasive Assessment of Tissue Engineered Human Oral Mucosa
    Mary Ann Mycek; Fiscal Year: 2012
    ..layer(s) of the EVPOME: SPECIFIC AIMS: (1) Test the efficacy of a microfluidic on-line electrophoresis immunoassay on a chip to assay three constitutively secreted proteins of oral keratinocytes important in graft function and ..
  22. Sex-Dependent PTHrP Processing and Lung Cancer Survival
    Randolph H Hastings; Fiscal Year: 2013
    ..Processing will be assessed by region-specific immunoassay, in vitro PTHrP proteolysis assays and mass spectrometry...
  23. Cervical Maturity, Mucosal Immunity, and Chlamydia trachomatis infection
    Loris Y Hwang; Fiscal Year: 2012
    ..to gain knowledge of basic and mucosal immunology;3) to gain practical experience and knowledge of immunoassay techniques to measure mucosal immune factors;4) to become proficient in advanced technologies for manipulation ..
  24. Low Cost Multi-pathogen Laser Diagnostic for HIV and AIDS Co-Infections
    CHRISTOPHER JOHN MYATT; Fiscal Year: 2011
    ..Photonics Corporation proposes to continue development of a low-cost, point-of-care, multiplexed fluorescence immunoassay system for the diagnosis of HIV and common co-infections from a single sample...
  25. A New Cytokine-Based Immunoassay for the Diagnosis of Lyme Disease
    Linda K Bockenstedt; Fiscal Year: 2013
    ..As a strategy that complements current serologic tests, the whole blood cytokine assay will improve the combined sensitivity and specificity of LD diagnostics, especially in situations in which the serologic tests alone under-perform. ..
  26. Point-of-care immunoassay for diagnosis of histoplasmosis in HIV/AIDS
    Sean Bauman; Fiscal Year: 2012
    ..An alternative approach is an immunoassay to detect H. capsulatum polysaccharide in serum or urine...
  27. PalmStat: a self-contained point of care diagnostic platform
    Michael B Prystowsky; Fiscal Year: 2011
    ..Traditional Point of Care single use cartridge tests are lateral flow immunoassay tests where the capture antibody is labeled with a chromagen, which allows for a positive reaction to be read ..
  28. A bioelectronic assay for PSA activity
    Dimitra Georganopoulou; Fiscal Year: 2010
    ..Thus, the major problem with screening for serum PSA by immunoassay is the high false positive rate caused by BPH which places a large burden on the healthcare system and leads to ..
  29. Interaction of Vitamin D &Vitamin A with Lung Cancer Risk in Non-Smoking Females
    Marian L Neuhouser; Fiscal Year: 2013
    ..08. We will use a chemiluminescent immunoassay to measure 25(OH)D and high-performance liquid chromatography with UV detector to measure retinyl esters and ..
  30. NOVEL BIOMARKERS FOR MECHANISTIC DIAGNOSIS OF THE ANTIPHOSPHOLIPID SYNDROME
    JACOB RAND; Fiscal Year: 2010
    ..new and related biomarkers for a thrombogenic APS mechanism: 1) the annexin A5 resistance (A5R) assay and 2) an immunoassay for antibodies to domain I of beta-2-glycoprotein I (anti-D1), an epitope that correlates with Annexin A5 ..
  31. Multiplexed Bead-based In Vitro Pediatric Allergy Test
    Mark Lim; Fiscal Year: 2013
    ..contrast, AmberGen has recently succeeded in developing a high sensitivity, high-throughput, low cost multiplex immunoassay approach by adapting the VeraCode(tm) digital holographic micro-bead technology developed by Illumina for ..
  32. Novel diagnostics and therapeutics for caliciviruses
    MARY KOLB ESTES; Fiscal Year: 2013
    ..universal primers to detect the viral RNA genome are not available, and no rapid, broadly-reactive enzyme immunoassay is approved in the US...
  33. Peptide therapeutics for inhibiting abdominal adhesions
    CYNTHIA ELLEN LANDER; Fiscal Year: 2012
    ..Conduct preliminary safety testing of MK2i in vivo. a. Develop an immunoassay for MK2i. b. Conduct pharmacodynamic studies to determine the effect of MK2i on major physiological systems. c...
  34. The STOP Study
    TRACEY PACKER; Fiscal Year: 2013
    ..To evaluate the yield, cost-effectiveness, and feasibility of screening for AHI with a fourth-generation enzyme immunoassay (EIA) in high-risk/high-incidence settings compared to pooled NAAT. 2...
  35. Novel Biomarkers for Early Detection of Aspergillosis
    Ram Ramabhadran; Fiscal Year: 2012
    ..The GM assay is an immunoassay where structurally similar cell wall mimicking antibiotics which do not contain lipids are well-known to lead ..
  36. Sensitive Measurement of Cardiac Troponin I at Point of Care: The Silicon BioDevi
    OCTAVIAN FLORESCU; Fiscal Year: 2012
    ..In this project we will produce and demonstrate a fully integrated POC immunoassay platform, based on a CMOS microchip, that is as easy to use as an over the counter pregnancy test and as ..
  37. Signaling in Airway Inflammation
    Roberto P Garofalo; Fiscal Year: 2013
    ..Internal and External), and providing biostatistical support to the projects;and a Viral Tissue Culture and Immunoassay Core (VTCIC, Core B), an established core responsible for high-quality viral and cell culture preparations, Bio-..
  38. Rapid Prototyping Method and Design Library for Universal POC Application
    VINCENT JEN JR GAU; Fiscal Year: 2010
    ..establish a design library to accommodate the most common molecular analysis assays including genetic testing, immunoassay and electrolyte detection...
  39. Novel Lipoproteins, Cardiometabolic Risk, and Statin Therapy
    Samia Mora; Fiscal Year: 2012
    ..We propose to conduct a comprehensive study of novel lipoproteins (immunoassay-measured apolipoproteins and nuclear magnetic resonance-measured lipoproteins) in relation to incident CVD and ..
  40. TRANSGENETIC STUDIES OF PRION DISEASE IN CERVIDS
    Glenn C Telling; Fiscal Year: 2013
    ..strains differ with respect to protease-sensitive PrPSc, and assess the sensitivity of Conformational Dependent Immunoassay, Fourier-transform infrared spectroscopy, luminescent conjugated polymers, and Protein Misfolding Cyclic ..
  41. Plasma Osteoprotegerin and Adverse Outcomes in CHD Patients
    Iftikhar J Kullo; Fiscal Year: 2013
    ..Using an immunoassay previously established in the applicant's laboratory, we will measure plasma OPG in 3634 patients with ..
  42. Ultrasensitive Detection of Hepatitis Viruses by Immunoassay Amplification
    Boris Rotman; Fiscal Year: 2013
    ..Project Description ..
  43. Detection and Diagnosis of Pancreatic Cancer
    David Gold; Fiscal Year: 2009
    ..Our recent developments employing an in vitro enzyme immunoassay to quantitate PAM4-reactive antigen in the blood of patients, appears quite promising for detection of ..
  44. Meso Scale Discovery Sector 6000 Imager
    CHARLES ALFRED STANLEY; Fiscal Year: 2010
    ..the acquisition of a Meso Scale Discovery SECTOR 6000 Imager for the detection of biomarkers by direct sandwich immunoassay in single and multiplex formats...
  45. Validation of an Antibody Test for Early Diagnosis of Ovarian Cancer
    Michael A Tainsky; Fiscal Year: 2013
    ..We used protein microarrays as a massively parallel immunoassay system to identify the proteins coded by the T7 bacteriophage cDNA libraries that bind to antibodies ..
  46. Magnetoresistive Sensor Platform for Parallel Cancer Marker Detection
    Marc D Porter; Fiscal Year: 2013
    ..the University of Utah seeks to create a new nanotechnology-based platform - the multiplexed magnetoresistive immunoassay (mMRIA) system - for the early detection of cancer...
  47. Development of a Hand-Held Cancer Biomarker Monitor
    Guodong Liu; Fiscal Year: 2011
    ..cancer biomarker monitor based on imunochromatographic technology and nanoparticle based electrochemical immunoassay for rapid, sensitive, and low- cost detection of cancer biomarkers in human blood samples...
  48. Rapid Point-of-Care Test to Detect Bacteriuria &Confirm Urinary Tract Infection
    MARK GEISBERG; Fiscal Year: 2012
    ..The proposed test kit will be a lateral flow immunoassay, similar to a home pregnancy test...
  49. Innovative Assay Development for the Diagnosis of Invasive Aspergillosis
    Marc D Porter; Fiscal Year: 2012
    ..fumigatus. (2) To identify and optimize multiplexed mAb combinations for a sandwich immunoassay based on surface enhanced Raman scattering (SERS), and mAb-coated gold nanoparticle labels...
  50. Antigen Immunoassay for Diagnosis of Coccidioidomycosis
    Sean Bauman; Fiscal Year: 2013
    ..The goal of this project is an immunoassay that will use serum or urine as a sample to diagnose coccidioidomycosis...
  51. Clade C-specific HIV assay to distinguish true infection from vaccine-induced ser
    ANDREW E LEVIN; Fiscal Year: 2013
    ..The goal of the Phase I project is to demonstrate feasibility of a clade C-specific HIV-1 immunoassay ("HIV Selectest") capable of distinguishing true HIV infection from VISP with high sensitivity and specificity...
  52. R&D- MEDICAL: BIOMEDICAL (BASIC RESEARCH)
    Andrew Levin; Fiscal Year: 2012
    This project is aimed at the development of an enzyme immunoassay (ELISA) for screening blood for antibodies to Babesia microti...
  53. New Multiplexed Quantitative Detection of Pluripotency and Germ Layer Proteins
    Bradley H Garcia; Fiscal Year: 2011
    ..and early germ layer differentiation, and then formatting these in a high throughput, low-cost multiplexed immunoassay format in a frameless microarray that is supported by one touch user friendly software for data analysis and ..
  54. High Throughput Immunoassay for Flagellin
    Eva King; Fiscal Year: 2013
    ..The goal of this SBIR Phase I project is to develop a high throughput immunoassay for flagellin, capable of analyzing several hundred samples per week...
  55. Point-of-Care HIV Antigen/Antibody Diagnostic Device
    Michael J Lochhead; Fiscal Year: 2011
    ..I proposal will focus on development of a novel, multiple particle approach for a HIV-1 p24 antigen sandwich immunoassay that will deliver clinically relevant limits of detection...
  56. Prevalence and immunogenicity of HNA-3a and -3b antibodies and antigens
    RICHARD HERBERT ASTER; Fiscal Year: 2012
    ..and specific for anti-CTL2 detection will be produced in quantity, formatted into a prototype solid phase immunoassay, and used to screen 7,000 serum samples from transfused, non-transfused and parous males and females to ..
  57. Imaging Based Dosimetry for Individualized Internal Emitter Therapy
    Yuni K Dewaraja; Fiscal Year: 2013
    ..g., Ki-67, p53 and FLT3-L from immunohistochemistry/immunoassay);and 4) Develop a mechanistic model (with and without modification for low dose hyper-radiosensitivity) to ..
  58. Function of semaphorin3a in diabetic nephropathy
    Alda Tufro; Fiscal Year: 2013
    ..urine and plasma sema3a levels will be measured in T1D and T2D human cohorts by quantitative enzyme-linked immunoassay. Aim 2 will examine how the sema3a-plexinA1 pathway regulates podocyte signaling and F-actin dynamics by ..
  59. A Low Cost, Accurate, and Portable Detection Method for Contaminant Levels in Foo
    Jiahuan Ding; Fiscal Year: 2012
    ..In Phase 1, Diagtronix aims to establish proof-of-concept for its unique bio-finFET immunoassay technology, for ultrasensitive, rapid, low-cost, and label-free quantification of various food contaminants ..
  60. Immunoassay for diagnosis of Babesia infection
    ANDREW E LEVIN; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): This project is aimed at development of an enzyme immunoassay (EIA) for antibodies to Babesia for use as a clinical diagnostic test...
  61. A high-sensitivity low-cost multiplexed immunoassay platform based on 2-dimension
    Paul Yager; Fiscal Year: 2013
    ....
  62. Saliva Analyzer for Diagnosing SRD Patient Drug Use
    Stuart Farquharson; Fiscal Year: 2012
    ..employing urine- based drug tests. For such tests, a sample is screened with immunoassay test kits, and if a drug is present, it is confirmed and quantified by gas chromatography - mass spectrometry (..
  63. Mass Spectrometry Tools in Pursuit of Salivary Biomarkers of Sjogren's Syndrome
    Steven C Hall; Fiscal Year: 2013
    ..the discovery and verification phases of the biomarker discovery process with development of a clinical immunoassay and validation of a marker panel as the second stage, i.e., outside the scope of the planned experiments...
  64. Plasma biomarkers for Parkinson's disease
    CATHERINE DANHUA PAN; Fiscal Year: 2011
    ..In this proposal, we will apply both mass spectrometry- and immunoassay-based quantitative methods in searching and quantitatively detecting panels of biomarker unique to PD and PD ..
  65. Preclinical assay development of a novel Colon Cancer Marker (CCSA-4)
    JACK CLEMENS; Fiscal Year: 2007
    ..we will (1) continue to characterize monoclonal antibodies to the CCSA-4 epitopes and optimize the immunoassay in order to apply it to larger clinical studies; (2) validate analytical performance of the optimized ..
  66. SIMULTANEOUS SCREENING FOR A/H3N2, A/H1N1, A/H5N1 AND B INFLUENZA VIRUSES
    Erica Dawson; Fiscal Year: 2009
    ..Currently, most health labs rely on a fluorescence-based immunoassay to screen for H3 or H1 viruses, with no confirmation of the neuraminidase subtype and no current capabilities ..
  67. Mannose binding lectin and outcomes following CABG
    GREGORY L contact STAHL; Fiscal Year: 2010
    ..the lectin complement pathway (LCP) in human diseases, we developed and validated a novel fluorochrome-linked immunoassay (FLISA) to measure the functional aspects of the lectin complement pathway to the level of C3 cleavage in human ..
  68. THROMBOPOIETIN: IMMUNOASSAY AND CHARACTERIZATION
    TED MC DONALD; Fiscal Year: 1993
    ....
  69. CLOSTRIDIUM DIFFICILE INFECTION
    JERGANG CHANG; Fiscal Year: 1991
    ..the efficacy of microbiological isolation and identification with that of detection of toxin-A by enzyme immunoassay and toxin-B by tissue culture cytotoxicity assay, (3) To study flora changes during C...
  70. AMPLIFIED IMMUNOASSAY OF BACTERIA AND RECOVERY
    SAGYPASH SADIEV; Fiscal Year: 1999
    A new technique for amplified immunoassay will be developed for sensitive measurement of bacteria under conditions which do not destroy them, coupled with the isolation of these bacteria from the immunoassay matrices...
  71. SERUM OSTEOCLASTIC ACID PHOSPHATASE IMMUNOASSAY
    KIN HING LAU; Fiscal Year: 1993
    ..Consequently, the investigators propose in this application to develop an immunoassay for osteoclastic TrACP from giant cell bone tumors...
  72. BIOCHEMISTRY OF MAST CELL SECRETORY GRANULE ENZYMES
    Lawrence Schwartz; Fiscal Year: 1999
    ..Specific aims concerning human tryptase are four-fold. First, a more sensitive and clinically useful immunoassay for tryptase will be developed as a precise but somewhat insensitive indication of mast cell involvement in ..
  73. ALDOSTERONE, RENIN AND PROSTAGLANDINS IN MAN
    Richard Horton; Fiscal Year: 1980
    ..Recent work in our laboratory has lead to a validated and accurate PGE immunoassay useful in human urine and blood...
  74. Development of immunoassay to measure a novel TNF-like protein
    Sunghee Kim; Fiscal Year: 2007
    ..This prompted us to report a prototype immunoassay to demonstrate the feasibility of measuring soluble TL1A released in human cell culture supernatant...
  75. PGA antigenemia for early diagnosis of anthrax
    Thomas Kozel; Fiscal Year: 2009
    ..by an anti-phagocytic capsule that is composed of poly-y-D-glutamic acid (PGA), raising the possibility than immunoassay for PGA could be a means for early, specific, rapid and inexpensive diagnosis of anthrax...
  76. Mitochondrial dysfunction in HAART: Point of care tests
    Michael F Marusich; Fiscal Year: 2010
    ..A set of dipstick immunoassay tests developed in Phase I have been used in clinical studies to show that OXPHOS deficits in both peripheral ..
  77. Screening Pretest for HNPCC
    Jeremy Fields; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): Our broad, long-term objective for Phase II is to develop an immunoassay to diagnose individuals carrying a hereditary nonpolyposis colon cancer (HNPCC) trait. There are >2...
  78. Development of Superagonist Analogs of Recombinant Human TSH for Imaging and Ther
    Bruce Weintraub; Fiscal Year: 2009
    ..We also plan to develop and validate a novel immunoassay for the detection of TSH analogs in unpurified rodent plasma that quantifies accurately and identically to ..