biosensing techniques

Summary

Summary: Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.

Top Publications

  1. ncbi Genetically encoded fluorescent indicator for intracellular hydrogen peroxide
    Vsevolod V Belousov
    Laboratory of Genes for Regeneration, Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya Street 16 10, Moscow 117997, Russia
    Nat Methods 3:281-6. 2006
  2. doi Biosensing with plasmonic nanosensors
    Jeffrey N Anker
    Chemistry Department, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208 3113, USA
    Nat Mater 7:442-53. 2008
  3. ncbi Spatiotemporal dynamics of RhoA activity in migrating cells
    Olivier Pertz
    University of North Carolina at Chapel Hill, Department of Pharmacology and Lineberger Cancer Center, Chapel Hill, North Carolina 27599, USA
    Nature 440:1069-72. 2006
  4. ncbi Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species
    Y Cui
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 293:1289-92. 2001
  5. pmc Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
    Amy E Palmer
    Department of Pharmacology and Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 101:17404-9. 2004
  6. doi Membrane phosphatidylserine regulates surface charge and protein localization
    Tony Yeung
    Division of Cell Biology, Hospital for Sick Children, Toronto M5G 1X8, Canada
    Science 319:210-3. 2008
  7. ncbi Nanostructures in biodiagnostics
    Nathaniel L Rosi
    Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208 3113, USA
    Chem Rev 105:1547-62. 2005
  8. pmc Development of an optimized backbone of FRET biosensors for kinases and GTPases
    Naoki Komatsu
    Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Kyoto 606 8501, Japan
    Mol Biol Cell 22:4647-56. 2011
  9. doi An electrochemical competitive biosensor for ochratoxin A based on a DNA biotinylated aptamer
    Laura Bonel
    Institute of Environmental Sciences IUCA, University of Zaragoza, Zaragoza, Spain
    Biosens Bioelectron 26:3254-9. 2011
  10. doi Sensitive optical biosensors for unlabeled targets: a review
    Xudong Fan
    Department of Biological Engineering, University of Missouri, 240D, Bond Life Sciences Center, 1201 E Rollins Street, Columbia, Missouri 65211, USA
    Anal Chim Acta 620:8-26. 2008

Research Grants

Detail Information

Publications343 found, 100 shown here

  1. ncbi Genetically encoded fluorescent indicator for intracellular hydrogen peroxide
    Vsevolod V Belousov
    Laboratory of Genes for Regeneration, Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya Street 16 10, Moscow 117997, Russia
    Nat Methods 3:281-6. 2006
    ..Moreover, sensitivity of the probe was sufficient to observe H(2)O(2) increase upon physiological stimulation. Using HyPer we detected temporal increase in H(2)O(2) in the cytoplasm of PC-12 cells stimulated with nerve growth factor...
  2. doi Biosensing with plasmonic nanosensors
    Jeffrey N Anker
    Chemistry Department, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208 3113, USA
    Nat Mater 7:442-53. 2008
    ..This review highlights several exceptionally promising research directions and discusses how diverse applications of plasmonic nanoparticles can be integrated in the near future...
  3. ncbi Spatiotemporal dynamics of RhoA activity in migrating cells
    Olivier Pertz
    University of North Carolina at Chapel Hill, Department of Pharmacology and Lineberger Cancer Center, Chapel Hill, North Carolina 27599, USA
    Nature 440:1069-72. 2006
    ..Our data therefore show that different extracellular cues induce distinct patterns of RhoA signalling during membrane protrusion...
  4. ncbi Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species
    Y Cui
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 293:1289-92. 2001
    ..The small size and capability of these semiconductor nanowires for sensitive, label-free, real-time detection of a wide range of chemical and biological species could be exploited in array-based screening and in vivo diagnostics...
  5. pmc Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
    Amy E Palmer
    Department of Pharmacology and Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 101:17404-9. 2004
    ....
  6. doi Membrane phosphatidylserine regulates surface charge and protein localization
    Tony Yeung
    Division of Cell Biology, Hospital for Sick Children, Toronto M5G 1X8, Canada
    Science 319:210-3. 2008
    ..More strongly cationic proteins, normally associated with the plasma membrane, relocalized to endocytic compartments when the plasma membrane surface charge decreased on calcium influx...
  7. ncbi Nanostructures in biodiagnostics
    Nathaniel L Rosi
    Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208 3113, USA
    Chem Rev 105:1547-62. 2005
  8. pmc Development of an optimized backbone of FRET biosensors for kinases and GTPases
    Naoki Komatsu
    Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Kyoto 606 8501, Japan
    Mol Biol Cell 22:4647-56. 2011
    ..Furthermore, this backbone enabled us to develop novel FRET biosensors for several kinases of RSK, S6K, Akt, and PKC and to perform quantitative evaluation of kinase inhibitors in living cells...
  9. doi An electrochemical competitive biosensor for ochratoxin A based on a DNA biotinylated aptamer
    Laura Bonel
    Institute of Environmental Sciences IUCA, University of Zaragoza, Zaragoza, Spain
    Biosens Bioelectron 26:3254-9. 2011
    ..78-8.74 ng mL(-1) and a limit of detection of 0.07±0.01 ng mL(-1), and was accurately applied to extracts of certified and spiked wheat samples with an RSD lower than about 8%...
  10. doi Sensitive optical biosensors for unlabeled targets: a review
    Xudong Fan
    Department of Biological Engineering, University of Missouri, 240D, Bond Life Sciences Center, 1201 E Rollins Street, Columbia, Missouri 65211, USA
    Anal Chim Acta 620:8-26. 2008
    ..Examples of detecting various types of biomolecules will be presented. Wherever possible, the sensing performance of each optical structure will be evaluated and compared in terms of sensitivity and detection limit...
  11. ncbi Detection of quorum-sensing N-acyl homoserine lactone signal molecules by bacterial biosensors
    Laura Steindler
    Bacteriology Group, International Centre for Genetic Engineering and Biotechnology, Padriciano, Trieste, Italy
    FEMS Microbiol Lett 266:1-9. 2007
    ..This article reviews and discusses the currently available bacterial biosensors which can be used in detecting and studying the different AHLs...
  12. pmc Aptamers and their biological applications
    Kyung Mi Song
    Department of Chemistry, Pohang University of Science and Technology, San31, Hyoja Dong, Pohang, Gyeongbuk 790 784, Korea
    Sensors (Basel) 12:612-31. 2012
    ..In summary, aptamers are promising materials for diverse areas, not just as alternatives to antibodies, but as the core components of medical and analytical equipment...
  13. ncbi Improving biosensor analysis
    D G Myszka
    Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84132, USA
    J Mol Recognit 12:279-84. 1999
    ..It is possible to globally fit high-quality biosensor data with simple bimolecular reaction models, which validates the technology as a biophysical tool for interaction analysis...
  14. pmc A sensing array of radically coupled genetic 'biopixels'
    Arthur Prindle
    Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA
    Nature 481:39-44. 2012
    ....
  15. pmc Imaging cytosolic NADH-NAD(+) redox state with a genetically encoded fluorescent biosensor
    Yin Pun Hung
    Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
    Cell Metab 14:545-54. 2011
    ..Furthermore, using high-content image analysis, we monitored NADH responses to PI3K pathway inhibition in hundreds of live cells. As an NADH reporter, Peredox should enable better understanding of bioenergetics...
  16. doi Fluorescent protein-based redox probes
    Andreas J Meyer
    Heidelberg Institute for Plant Science, Heidelberg University, Heidelberg, Germany
    Antioxid Redox Signal 13:621-50. 2010
    ..In addition, redox biosensor transgenic model organisms offer extended opportunities for dynamic in vivo imaging of redox processes...
  17. doi Biosensors for cancer markers diagnosis
    Ibtisam E Tothill
    Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK 43 0AL England, UK
    Semin Cell Dev Biol 20:55-62. 2009
    ..This review provides an overview of the biosensor technology available today, areas which are currently being developed and researched for cancer markers diagnosis-and a consideration of future prospects for the technology...
  18. doi Direct electrochemistry of glucose oxidase and biosensing for glucose based on graphene
    Changsheng Shan
    State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, and Graduate University of the Chinese Academy of Sciences, Changchun 130022, PR China
    Anal Chem 81:2378-82. 2009
    ....
  19. ncbi Intrinsic peroxidase-like activity of ferromagnetic nanoparticles
    Lizeng Gao
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China
    Nat Nanotechnol 2:577-83. 2007
    ..The stability, ease of production and versatility of these nanoparticles makes them a powerful tool for a wide range of potential applications in medicine, biotechnology and environmental chemistry...
  20. ncbi Quantum dot bioconjugates for imaging, labelling and sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Nat Mater 4:435-46. 2005
    ..The potential of QDs in biology has just begun to be realized and new avenues will arise as our ability to manipulate these materials improves...
  21. doi Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A
    Cheng Yang
    State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China
    Biosens Bioelectron 32:208-12. 2012
    ..As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10 nM, showing a limit of detection of 2.5 nM...
  22. pmc Three dimensional gait analysis using wearable acceleration and gyro sensors based on quaternion calculations
    Shigeru Tadano
    Division of Human Mechanical Systems and Design, Faculty of Engineering, Hokkaido University, Sapporo, Japan
    Sensors (Basel) 13:9321-43. 2013
    ..The average RMSE and correlation coefficient (CC) were 10.14 deg and 0.98, 7.88 deg and 0.97, 9.75 deg and 0.78 for the hip, knee and ankle flexion angles, respectively. ..
  23. pmc A genetically encoded fluorescent reporter of ATP:ADP ratio
    Jim Berg
    Department of Neurobiology, Harvard Medical School, 220 Longwood Ave, Boston, Massachusetts 02115, USA
    Nat Methods 6:161-6. 2009
    ..The engineered sensor, Perceval, can be used to monitor the ATP:ADP ratio during live-cell imaging...
  24. doi Highly sensitive ochratoxin A impedimetric aptasensor based on the immobilization of azido-aptamer onto electrografted binary film via click chemistry
    Akhtar Hayat
    BIOMEM, Universite de Perpignan, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France
    Talanta 103:14-9. 2013
    ..The increase in electron-transfer resistance (R(et)) values due to the specific aptamer-OTA interaction was proportional to the concentration of OTA in a range between 1.25 ng/L and 500 ng/L, with a detection limit of 0.25 ng/L...
  25. pmc Imaging the coordination of multiple signalling activities in living cells
    CHRISTOPHER M WELCH
    Department of Pharmacology and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Rev Mol Cell Biol 12:749-56. 2011
    ..The precision and scope of this approach has been greatly extended by novel computational approaches (referred to as computational multiplexing) that can reveal relationships between network nodes imaged in separate cells...
  26. pmc Highly ordered nanowire arrays on plastic substrates for ultrasensitive flexible chemical sensors
    Michael C McAlpine
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Nat Mater 6:379-84. 2007
    ..The excellent sensing performance coupled with bendable plastic could open up opportunities in portable, wearable or even implantable sensors...
  27. doi Bacteriophage based probes for pathogen detection
    Amit Singh
    Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada
    Analyst 137:3405-21. 2012
    ..This review focuses on the description of pathogen detection approaches based on immobilized phage virions as well as pure recombinant RBPs. Specific advantages of RBP-based molecular probes are also discussed...
  28. doi Deconvolution of complex G protein-coupled receptor signaling in live cells using dynamic mass redistribution measurements
    Ralf Schröder
    Molecular, Cellular, and Pharmacobiology Section, Institute of Pharmaceutical Biology, University of Bonn, Bonn, Germany
    Nat Biotechnol 28:943-9. 2010
    ..Our results suggest that DMR technology will have a substantial impact on systems biology and systems pharmacology as well as for the discovery of drugs with novel mechanisms...
  29. ncbi Direct electron transfer between copper-containing proteins and electrodes
    Sergey Shleev
    Department of Analytical Chemistry, Lund University, P O Box 124, SE 221 00 Lund, Sweden
    Biosens Bioelectron 20:2517-54. 2005
    ..It is shown that long-range electron transfer between these enzymes and electrodes can be established, and the mechanistic schemes of the DET processes are proposed...
  30. pmc Perspective on optical biosensors and integrated sensor systems
    Frances S Ligler
    Naval Research Laboratory, Center for Bio Molecular Science and Engineering, 455 Overlook Avenue South West, Washington, DC 20375, USA
    Anal Chem 81:519-26. 2009
    ..Examples of innovations are identified that will lead to smaller, faster, cheaper optical biosensor systems with capacity to provide effective and actionable information...
  31. pmc Comparative advantages of mechanical biosensors
    J L Arlett
    Kavli Nanoscience Institute and Departments of Physics, Applied Physics, and Bioengineering, California Institute of Technology, Pasadena, CA 91125, USA
    Nat Nanotechnol 6:203-15. 2011
    ..We also discuss the need for a greater understanding of analyte-sensor interactions on the nanoscale and of stochastic processes in the sensing environment...
  32. doi PVP-coated graphene oxide for selective determination of ochratoxin A via quenching fluorescence of free aptamer
    Linfeng Sheng
    State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China
    Biosens Bioelectron 26:3494-9. 2011
    ..This sensor has also been challenged by testing 1% red wine containing buffer solution spiked with a series of concentration of ochratoxin A...
  33. ncbi Photonic crystal surface waves for optical biosensors
    Valery N Konopsky
    Anal Chem 79:4729-35. 2007
    ..The detection limit is approximately 20 fg of the analyte on the probed spot of the surface...
  34. pmc Glucose biosensors: an overview of use in clinical practice
    Eun Hyung Yoo
    Department of Laboratory Medicine, Konyang University Hospital, College of Medical Science, Konyang University, Daejon, Korea
    Sensors (Basel) 10:4558-76. 2010
    ..This article reviews the brief history, basic principles, analytical performance, and the present status of glucose biosensors in the clinical practice...
  35. ncbi Electrochemical DNA sensors
    T Gregory Drummond
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Nat Biotechnol 21:1192-9. 2003
    ....
  36. doi Manual closed-loop insulin delivery in children and adolescents with type 1 diabetes: a phase 2 randomised crossover trial
    Roman Hovorka
    Department of Paediatrics, University of Cambridge, Cambridge, UK
    Lancet 375:743-51. 2010
    ..Closed-loop systems link continuous glucose measurements to insulin delivery. We aimed to establish whether closed-loop insulin delivery could control overnight blood glucose in young people...
  37. doi Aptamer-based colorimetric biosensing of Ochratoxin A using unmodified gold nanoparticles indicator
    Cheng Yang
    State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China
    Biosens Bioelectron 26:2724-7. 2011
    ..The linear range of the colorimetric aptasensor covered a large variation of OTA concentration from 20 to 625 nM and the detection limit of 20 nM (3σ) was obtained...
  38. doi Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors
    Hui Wang Ai
    University of Alberta, Department of Chemistry, 11227 Saskatchewan Drive, Edmonton, Alberta T6G 2G2, Canada
    Nat Methods 5:401-3. 2008
    ..Owing to their distinct excitation profiles, each FRET biosensor can be ratiometrically imaged in the presence of the other...
  39. ncbi Aptamer-based biosensors for the detection of HIV-1 Tat protein
    S Tombelli
    Universita degli Studi di Firenze, Dipartimento di Chimica Polo Scientifico di Sesto Fiorentino Via della Lastruccia 3, 50019 Sesto Fiorentino FI, Italy
    Bioelectrochemistry 67:135-41. 2005
    ..A protein very similar to Tat, Rev protein, has been used as negative control. The two biosensors both were very reproducible in the immobilisation and the binding steps. The selectivity was high in both cases...
  40. pmc Real-time biomolecular binding detection using a sensitive photonic crystal biosensor
    Yunbo Guo
    Center for Ultrafast Optical Science, Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan 48109, USA
    Anal Chem 82:5211-8. 2010
    ..By all important metrics for sensitivity, we anticipate this photonic crystal biosensor will provide new capabilities for highly sensitive measurements of biomolecular binding...
  41. ncbi Present and future of surface plasmon resonance biosensors
    Jiri Homola
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Anal Bioanal Chem 377:528-39. 2003
    ..This paper reviews fundamentals of SPR affinity biosensors and discusses recent advances in development and applications of SPR biosensors...
  42. ncbi Ion-beam sculpting at nanometre length scales
    J Li
    Department of Physics Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 412:166-9. 2001
    ..We show that ion-beam sculpting can be used to fashion an analogous solid-state device: a robust electronic detector consisting of a single nanopore in a Si3N4 membrane, capable of registering single DNA molecules in aqueous solution...
  43. doi Graphene-based single-bacterium resolution biodevice and DNA transistor: interfacing graphene derivatives with nanoscale and microscale biocomponents
    Nihar Mohanty
    Chemical Engineering, Kansas State University, Manhattan, Kansas 66506, USA
    Nano Lett 8:4469-76. 2008
    ....
  44. pmc Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors
    Robert J Chen
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 100:4984-9. 2003
    ..This scheme, combined with the sensitivity of nanotube electronic devices, enables highly specific electronic sensors for detecting clinically important biomolecules such as antibodies associated with human autoimmune diseases...
  45. doi Biological and chemical sensors based on graphene materials
    Yuxin Liu
    Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore
    Chem Soc Rev 41:2283-307. 2012
    ..Finally, the perspective and current challenges of graphene sensors are outlined (312 references)...
  46. ncbi Label-free screening of bio-molecular interactions
    Matthew A Cooper
    Cambridge Centre for Molecular Recognition, University Chemical Laboratory, Lensfield Road, CB2 1EW, Cambridge, UK
    Anal Bioanal Chem 377:834-42. 2003
    ....
  47. doi Aptamer-based optical biosensor for rapid and sensitive detection of 17β-estradiol in water samples
    Nimet Yildirim
    Bioengineering Program, Northeastern University, Boston, United States
    Environ Sci Technol 46:3288-94. 2012
    ..This portable sensor system can be potentially applied for on-site real-time inexpensive and easy-to-use monitoring of 17β-estradiol in environmental samples such as effluents or water bodies...
  48. doi Ultrasensitive flexible graphene based field-effect transistor (FET)-type bioelectronic nose
    Seon Joo Park
    World Class University program of Chemical Convergence for Energy and Environment, School of Chemical and Biological Engineering, Seoul National University, Seoul 151 742, Korea
    Nano Lett 12:5082-90. 2012
    ..3.44 × 10(14) and 1.47 × 10(14) M(-1), respectively. Additionally, the B-noses have long-term stability and excellent mechanical bending durability in flexible systems...
  49. doi Ultrasensitive one-step rapid detection of ochratoxin A by the folding-based electrochemical aptasensor
    Jingjing Wu
    School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, PR China
    Anal Chim Acta 753:27-31. 2012
    ..Study results showed that this electrochemical aptasensor could be a potential useful platform for on-site OTA measurement in real complex samples...
  50. doi Double-probe signal enhancing strategy for toxin aptasensing based on rolling circle amplification
    Ping Tong
    The State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, China
    Biosens Bioelectron 33:146-51. 2012
    ..2 pg mL(-1) with a dynamic range spanning more than 4 orders of magnitude. The proposed approach is tested to determine OTA in red wines and shows good application potential in real samples...
  51. doi Genetically encoded chloride indicator with improved sensitivity
    Olga Markova
    Institut de Neurobiologie de la Méditerranée INMED, INSERM U901, Parc Scientifique de Luminy, Marseille, France
    J Neurosci Methods 170:67-76. 2008
    ..This genetically encoded indicator offers a means for monitoring Cl and pH under different physiological conditions and high-throughput screening of pharmacological agents...
  52. ncbi Glucose biosensor based on the microcantilever
    Jianhong Pei
    Life Science Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 6123, USA
    Anal Chem 76:292-7. 2004
    ..Experiments were carried out under flow conditions. The common interferences for glucose detection in other detection schemes have been tested and have shown to have no effect on the measurement of blood glucose level by this technique...
  53. doi Biosensors for the detection of waterborne pathogens
    John T Connelly
    Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA
    Anal Bioanal Chem 402:117-27. 2012
    ..Further, we identify future areas of focus necessary to realize novel detection devices capable of supplanting the gold standards of today...
  54. pmc Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors
    Mingxing Ouyang
    Department of Bioengineering, Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, IL 61801, USA
    Proc Natl Acad Sci U S A 105:14353-8. 2008
    ..In summary, ECFP/YPet may serve as a general FRET pair for the development of highly sensitive biosensors to allow the determination of molecular hierarchies at subcellular locations in live cells...
  55. doi Recent advances in graphene-based biosensors
    Tapas Kuila
    Department of BIN Fusion technology, Chonbuk National University, Jeonju, Jeonbuk 561 756, Republic of Korea
    Biosens Bioelectron 26:4637-48. 2011
    ..Graphene based FETs have also been discussed in details. In all these cases, the biosensors performed well with low working potentials, high sensitivities, low detection limits, and long-term stabilities...
  56. doi Polyaniline Langmuir-Blodgett film based aptasensor for ochratoxin A detection
    Nirmal Prabhakar
    Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, Materials Physics and Engineering Division, National Physical Laboratory Council of Scientific and Industrial Research, Dr K S Krishnan Marg, New Delhi 110012, India
    Biosens Bioelectron 26:4006-11. 2011
    ..1 ng/ml in 15 min. The Apt-DNA/PANI-SA/ITO aptasensor can be reused ∼13 times. The binding or affinity constant (K(a)) of aptamer with OTA, calculated using Langmuir adsorption isotherm, is found be 1.21×10(7) M(-1)...
  57. ncbi Development of a set of simple bacterial biosensors for quantitative and rapid measurements of arsenite and arsenate in potable water
    Judith Stocker
    Swiss Federal Institute for Environmental Science and Technology, CH 8600 Dubendorf, Switzerland
    Environ Sci Technol 37:4743-50. 2003
    ..Therefore, the biosensor cells and protocols may offer a realistic alternative for measuring arsenic contamination in potable water...
  58. pmc Programmable nano-bio-chip sensors: analytical meets clinical
    Jesse V Jokerst
    The University of Texas at Austin, USA
    Anal Chem 82:1571-9. 2010
    ..To listen to a podcast about this article, please go to the Analytical Chemistry multimedia page at pubs.acs.org/page/ancham/audio/index.html .)...
  59. ncbi Biosensors based on electropolymerized films: new trends
    Serge Cosnier
    Laboratoire d Electrochimie Organique et de Photochimie Redox, UMR CNRS 5630, Institut de Chimie Moleculaire de Grenoble, FR CNRS 2607, Universite Joseph Fourier, BP 53, 38041, Grenoble Cedex 9, France
    Anal Bioanal Chem 377:507-20. 2003
    ....
  60. doi Aptamer-based molecular recognition for biosensor development
    Jing Zhou
    Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, 191 Auditorium Road, Storrs, CT 06269 3222, USA
    Anal Bioanal Chem 398:2471-80. 2010
    ..This review first concisely introduces methods for aptamer discovery including upstream selection and downstream truncation, then discusses aptamer-based biosensor development from the viewpoint of signal production...
  61. pmc Subthreshold regime has the optimal sensitivity for nanowire FET biosensors
    Xuan P A Gao
    Department of Physics, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Nano Lett 10:547-52. 2010
    ..Our work shows that optimization of NW-FET structure and operating conditions can provide significant enhancement and fundamental understanding for the sensitivity limits of NW-FET sensors...
  62. doi Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing
    Xinhuang Kang
    Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Biosens Bioelectron 25:901-5. 2009
    ....
  63. doi An overview of foodborne pathogen detection: in the perspective of biosensors
    Vijayalakshmi Velusamy
    Electronic and Computer Engineering Department, University of Limerick, Limerick, Ireland
    Biotechnol Adv 28:232-54. 2010
    ....
  64. doi Whole-cell aquatic biosensors
    Evgeni Eltzov
    Unit of Environmental Engineering, Faculty of Engineering Science, Ben Gurion University of the Negev, Beer Sheva, Israel
    Anal Bioanal Chem 400:895-913. 2011
    ..Examples of the use of a broad spectrum of whole-cell bioreporters in assessing a variety of environmental problems are summarized...
  65. doi Graphene/AuNPs/chitosan nanocomposites film for glucose biosensing
    Changsheng Shan
    State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate University of the Chinese Academy of Sciences, Changchun, PR China
    Biosens Bioelectron 25:1070-4. 2010
    ..The graphene/AuNPs/GOD/chitosan composites film shows prominent electrochemical response to glucose, which makes a promising application for electrochemical detection of glucose...
  66. doi An integrated microfluidic system for rapid diagnosis of dengue virus infection
    Yu Fang Lee
    Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan
    Biosens Bioelectron 25:745-52. 2009
    ..This integrated system may provide a powerful platform for the rapid diagnosis of dengue virus infection and other types of infectious diseases...
  67. doi Bactenecin 7 peptide fragment as a tool for intracellular delivery of a phosphorescent oxygen sensor
    Ruslan I Dmitriev
    Biochemistry Department, University College Cork, Cork, Ireland
    FEBS J 277:4651-61. 2010
    ..In addition, the responses to metabolic stimuli can be assessed in a wide range of cells, as can the levels of relative cell oxygenation under external hypoxia...
  68. doi Microbial biosensors: a review
    Liang Su
    Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, 191 Auditorium Road, Storrs, CT 06269, USA
    Biosens Bioelectron 26:1788-99. 2011
    ..Prospective strategies for the design of future microbial biosensors will also be discussed...
  69. doi Where microbiology meets microengineering: design and applications of reporter bacteria
    Jan Roelof van der Meer
    University of Lausanne, Department of Fundamental Microbiology, 1015 Lausanne, Switzerland
    Nat Rev Microbiol 8:511-22. 2010
    ..New trends for integrating synthetic biology and microengineering into the design of bacterial bioreporter platforms are also highlighted...
  70. ncbi Enzymatic biofuel cells for implantable and microscale devices
    Scott Calabrese Barton
    Department of Chemical Engineering, Columbia University, New York, New York 10027, USA
    Chem Rev 104:4867-86. 2004
  71. doi Review: Carbon nanotube based electrochemical sensors for biomolecules
    Christopher B Jacobs
    Dept of Chemistry, University of Virginia, PO Box 400319, Charlottesville, VA 22904, United States
    Anal Chim Acta 662:105-27. 2010
    ..Future challenges for the field include miniaturizing sensors, developing methods to use only a specific nanotube allotrope, and simplifying manufacturing...
  72. pmc Waveguide-mode sensors as aptasensors
    Subash C B Gopinath
    Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8562, Japan
    Sensors (Basel) 12:2136-51. 2012
    ..Herein, we provide an overview of the strategies and applications for aptamer-based analyses using waveguide-mode sensors...
  73. doi Nucleic acid aptamers against biotoxins: a new paradigm toward the treatment and diagnostic approach
    Lasse Holm Lauridsen
    School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, Queensland, Australia
    Nucleic Acid Ther 22:371-9. 2012
    ..In this review, we focus on nucleic acid aptamers developed against various biotoxins of plant, microorganism, or animal origin and show how these can be used in diagnostics (e.g., biosensors) and therapy...
  74. pmc Recent advances in bacteriophage based biosensors for food-borne pathogen detection
    Amit Singh
    Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada
    Sensors (Basel) 13:1763-86. 2013
    ....
  75. doi Novel genetically encoded biosensors using firefly luciferase
    Frank Fan
    ACS Chem Biol 3:346-51. 2008
    ..These and other luminescent biosensors will be useful for the sensitive detection of cellular physiology in research and drug discovery...
  76. doi The enhanced cyan fluorescent protein: a sensitive pH sensor for fluorescence lifetime imaging
    Sandrine Poea-Guyon
    Université of Paris Sud, Orsay, France
    Anal Bioanal Chem 405:3983-7. 2013
    ..By fusion of ECFP with the granular chromogranin A, we successfully measured the pH in secretory granules of PC12 cells, and we performed a kinetic analysis of intragranular pH variations in living cells exposed to ammonium chloride...
  77. pmc Bilirubin oxidase from Bacillus pumilus: a promising enzyme for the elaboration of efficient cathodes in biofuel cells
    Fabien Durand
    CRPP UPR 8641, Univ Bordeaux, F 33600, Pessac, France
    Biosens Bioelectron 35:140-6. 2012
    ..tsunodae BOD modified electrode. This makes BOD from B. pumilus an attractive new candidate for application in biofuel cells (BFCs) and biosensors...
  78. doi Enzyme-catalyzed O2 removal system for electrochemical analysis under ambient air: application in an amperometric nitrate biosensor
    Nicolas Plumeré
    Center for Electrochemical Sciences CES, Analytische Chemie, Ruhr Universitat Bochum, Universitatsstrasse 150, 44801 Bochum, Germany
    Anal Chem 84:2141-6. 2012
    ..For general application, the optimum catalyst is pyranose 2-oxidase since the oxidation product does not interfere with the biorecognition element...
  79. doi Six-color time-resolved Förster resonance energy transfer for ultrasensitive multiplexed biosensing
    Daniel Geisler
    Physical Chemistry, University of Potsdam, 14476 Potsdam Golm, Germany
    J Am Chem Soc 135:1102-9. 2013
    ..This novel technology will open new doors for multiple biomarker diagnostics as well as multiplexed real-time imaging and spectroscopy...
  80. doi Visualization of PKA activity in plasma membrane microdomains
    CHARLENE DEPRY
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Mol Biosyst 7:52-8. 2011
    ....
  81. pmc Immunochemical methods for ochratoxin A detection: a review
    Eline P Meulenberg
    ELTI Support VOF, Ambachtsweg 5, 6581 AX Malden, The Netherlands
    Toxins (Basel) 4:244-66. 2012
    ..Further, the application of the various formats to the detection and quantification of ochratoxin will be described, including the use of commercially available kits...
  82. ncbi Protein electrochemistry using aligned carbon nanotube arrays
    J Justin Gooding
    School of Chemical Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    J Am Chem Soc 125:9006-7. 2003
    ..The efficiency of the electron transfer through the SWNTs is demonstrated by electrodes modified with tubes cut to different lengths having the same electron-transfer rate constant...
  83. doi Cell-based electrochemical biosensors for water quality assessment
    Florence Lagarde
    University of Lyon, Laboratoire des Sciences Analytiques, UMR, CNRS, CPE, Villeurbanne, France
    Anal Bioanal Chem 400:947-64. 2011
    ..In some cases, it is also possible to achieve higher selectivities, even without cell modification, by operating at specific potentials where interferences are limited...
  84. ncbi Structure-switching signaling aptamers
    Razvan Nutiu
    Department of Biochemistry, McMaster Univ, Health Sciences Center, 1200 Main Street West, Hamilton, Ontario, L8N 3Z5, Canada
    J Am Chem Soc 125:4771-8. 2003
    ..Our design strategy is easy to generalize for any aptamer without prior knowledge of its secondary or tertiary structure, and should be suited for the development of aptamer-based reporters for real-time sensing applications...
  85. doi Biosensors and their applications in microbial metabolic engineering
    Fuzhong Zhang
    Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA
    Trends Microbiol 19:323-9. 2011
    ..This review will focus on recent developments in biosensor design, and their applications for controlling microbial behavior...
  86. doi Transparent, flexible, all-reduced graphene oxide thin film transistors
    Qiyuan He
    School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
    ACS Nano 5:5038-44. 2011
    ..The specific detection of avidin by using biotinylated all-rGO sensor is also successfully demonstrated...
  87. doi Application of peptide nucleic acid towards development of nanobiosensor arrays
    Ravindra P Singh
    Interdisciplinary Program of Integrated Biotechnology, Sogang University, 1 Sinsoo Dong, Mapo gu, Seoul 121 742, Republic of Korea
    Bioelectrochemistry 79:153-61. 2010
    ..This review paper discusses the various current aspects related with PNAs, making a new hot device in the commercial applications like nanobiosensor arrays...
  88. doi Versatile synthesis strategy for carboxylic acid-functionalized upconverting nanophosphors as biological labels
    Zhigang Chen
    Department of Chemistry and Laboratory of Advanced Materials, Fudan University, 220 Handan Road, Shanghai 200433, People s Republic of China
    J Am Chem Soc 130:3023-9. 2008
    ....
  89. doi Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide
    Ming Zhou
    State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People s Republic of China
    Anal Chem 81:5603-13. 2009
    ....
  90. ncbi Surface plasmon resonance biosensor for direct detection of antibody against Epstein-Barr virus
    Hana Vaisocherová
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 182 51 Prague, Czech Republic
    Biosens Bioelectron 22:1020-6. 2007
    ..It was demonstrated that the sensor is capable of detecting concentrations of anti-EBNA as low as 0.2 ng/ml (approximately 1 pM) both in buffer and 1% human serum and can be stored and regenerated for repeated use...
  91. pmc Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen
    Nazanin Saeidi
    School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore
    Mol Syst Biol 7:521. 2011
    ..These results suggest that E. coli carrying our synthetic genetic system may provide a novel synthetic biology-driven antimicrobial strategy that could potentially be applied to fighting P. aeruginosa and other infectious pathogens...
  92. pmc The use of wearable inertial motion sensors in human lower limb biomechanics studies: a systematic review
    Daniel Tik Pui Fong
    Department of Orthopaedics and Traumatology, Prince of Wales Hospital, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
    Sensors (Basel) 10:11556-65. 2010
    ..Thirty nine full papers and conference abstracts with related topics were included in this review. The type of sensor involved, data collection methods, study design, validation methods and its applications were reviewed...
  93. pmc Self-assembled combinatorial encoding nanoarrays for multiplexed biosensing
    Chenxiang Lin
    Department of Chemistry and Biochemistry and The Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
    Nano Lett 7:507-12. 2007
    ..Accurate control of the interprobe distances and solution-based binding reactions ensures fast target binding kinetics...
  94. ncbi Electro-chemiluminescent biosensing
    Christophe A Marquette
    Laboratoire de Genie Enzymatique et Biomoleculaire, Institut de Chimie et Biochimie Moleculaires et Supramoleculaires, Universite Lyon 1 CNRS 5246 ICBMS, Villeurbanne, France
    Anal Bioanal Chem 390:155-68. 2008
    ..The mechanism, principle, and experimental conditions of these two reactions are described. Then, for each category of analytical tools, experimental set-ups and performances are presented and discussed...
  95. doi Graphene fluorescence resonance energy transfer aptasensor for the thrombin detection
    Haixin Chang
    Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing 100084, People s Republic of China
    Anal Chem 82:2341-6. 2010
    ..The excellent performance of FRET aptasensor based on graphene will also be ascribed to the unique structure and electronic properties of graphene...
  96. ncbi Optical forward-scattering for detection of Listeria monocytogenes and other Listeria species
    Padmapriya P Banada
    Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA
    Biosens Bioelectron 22:1664-71. 2007
    ..monocytogenes and other Listeria species colonies grown on agar plates within 5-10 min analysis time...
  97. ncbi Redox-sensitive GFP in Arabidopsis thaliana is a quantitative biosensor for the redox potential of the cellular glutathione redox buffer
    Andreas J Meyer
    Heidelberg Institute of Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Plant J 52:973-86. 2007
    ..The results with roGFP2 as an artificial GRX target further suggest that redox-triggered changes of biologic processes might be linked directly to the glutathione redox potential via GRX as the mediator...
  98. doi Chemically immobilized T4-bacteriophage for specific Escherichia coli detection using surface plasmon resonance
    Sunil K Arya
    Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Canada
    Analyst 136:486-92. 2011
    ..The results of these studies have implications for the development of online bioassays for the detection of various food and water borne pathogens using the inherent selectivity of bacteriophage recognition...
  99. doi Sequence-specific recognition of DNA oligomer using peptide nucleic acid (PNA)-modified synthetic ion channels: PNA/DNA hybridization in nanoconfined environment
    Mubarak Ali
    Technische Universität Darmstadt, Fachbereich Material u Geowissenschaften, Fachgebiet Materialanalytik, Petersenstrasse 23, D 64287 Darmstadt, Germany
    ACS Nano 4:7267-74. 2010
    ..In this context, we envision that the single conical nanochannels functionalized with a PNA probe will provide a biosensing platform for the detection and discrimination of short single-stranded DNA oligomer of unknown sequence...
  100. doi Nanocapsules incorporating IgG Fc-binding domain derived from Staphylococcus aureus protein A for displaying IgGs on immunosensor chips
    Masumi Iijima
    Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa 464 8601, Japan
    Biomaterials 32:1455-64. 2011
    ..The BNC-coated sensor chip is very stable, and should prove useful for various immunosensor applications due to oriented immobilization of antibodies...
  101. pmc Fluorescence resonance energy transfer biosensors for cancer detection and evaluation of drug efficacy
    Shaoying Lu
    Department of Bioengineering and Beckman Institute for Advanced Science and Technology, Neuroscience Program, Center of Biophysics and Computational Biology, Institute of Genomic Biology, University of Illinois at Urbana Champaign, 405 N Mathews Avenue, Urbana, IL 61801, USA
    Clin Cancer Res 16:3822-4. 2010
    ..This biosensor allowed the detection of cancerous and drug-resistant cells, and the evaluation of kinase inhibitor efficacy. Future biosensor development and imaging can increasingly contribute to cancer diagnosis and therapeutics...

Research Grants59

  1. Biosensors for Environmental Contaminants
    MICHAEL BLAYLOCK; Fiscal Year: 2005
    ..In addition, demonstration of turf grass as a biosensor for soil lead is expected to stimulate development of low-cost biosensing techniques for other environmental health hazards.
  2. Rapid homogeneous antibody-based detection of proteins
    Tomasz Heyduk; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  3. Modeling Mass Transfer to Active Implants for Diabetes
    David Gough; Fiscal Year: 2009
    ..This project will examine the permeability of tissues surrounding implanted devices to assess substrate supply to the implant. This information may accelerate implementation of these therapies. ..
  4. Molecular Beacon Aptamer for Diagnostic Cancer Imaging
    Weihong Tan; Fiscal Year: 2003
    ..in molecular beacons; 2) Develop ultrasensitive optical fiber biosensors and advanced imaging and biosensing techniques based on the newly developed molecular beacon aptamer; 3) Explore the feasibility of using the molecular ..
  5. EXPLORATION OF NANOPARTICLE OPTICAL BISENSOR ARRAYS
    Steven Blair; Fiscal Year: 2003
    Research world-wide on biosensing techniques is motivated by numerous applications in clinical diagnostics...
  6. 5-Day Fiber-Coupled Sensor Interstitial Glucose
    Ralph Ballerstadt; Fiscal Year: 2006
    ..We believe the proposed sensor can provide a solution for short-term (3-5 days) continuous monitoring that will be accurate, reliable, and convenient for patient use. [unreadable] [unreadable] [unreadable]..
  7. Nanoparticle-Based Optical Sensor for Glucose Monitoring
    Ralph Ballerstadt; Fiscal Year: 2006
    ..abstract_text> ..
  8. QCM Quantification of Endocrine Disruptor Activity
    Xiangqun Zeng; Fiscal Year: 2004
    ..Given the U.S. congressional mandate and World Health Organization recommendations for endocrine disrupter testing of chemicals, we anticipate strong market demand for ERE biosensor technology. ..
  9. Fluorescence Sensor Probe for Transcutaneous Glucose Sensing
    Ralph Ballerstadt; Fiscal Year: 2008
    ..The overall result will be a healthier life for people with diabetes and lower medical costs for diabetes care for the society. [unreadable] [unreadable] [unreadable]..
  10. MOLECULAR BEACONS FOR PROTEIN DETECTION
    Tomasz Heyduk; Fiscal Year: 2005
    ..abstract_text> ..
  11. Microarrays for DNA binding proteins
    Tomasz Heyduk; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  12. Reducing the Disproportionate Burden of Orofacial Injury
    Vivek Shetty; Fiscal Year: 2007
    ..abstract_text> ..
  13. BIOSENSOR ARRAYS BASED ON SINGLE WALL CARBON NANOTUBES
    James F Rusling; Fiscal Year: 2010
    ..In addition to achieving our specific aims, this project should provide fundamental guidance for fabrication of sensitive microarray devices for a wide variety of biomedical applications. ..
  14. Biochemical Modulation of Nociceptive Circuits
    MARK JOHN ZYLKA; Fiscal Year: 2010
    ..Finally, we will test the hypothesis that this phosphatase modulates neuropathic pain behaviors in wild-type and phosphatase knockout mice. ..
  15. Macrophage specific magnetic-fluorescent nanoprobes
    Angelique Louie; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  16. A POLYMERIC DRUG DELIVERY SYSTEM FOR CANCER THERAPY
    JINDRICH H KOPECEK; Fiscal Year: 2010
    ..The concept of double-targeted macromolecular therapeutics provides a new paradigm for the design of efficient anticancer drugs for the treatment of ovarian cancer. ..
  17. Nonobiosensors for Probing Chemical Exposure and Metabolism Pathways of Individua
    Tuan Vo Dinh; Fiscal Year: 2009
    ....
  18. MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLY
    Lynne Cassimeris; Fiscal Year: 2006
    ..To address these issues, we will apply a number of fluorescence-based imaging methods combined with siRNA or injection of function-blocking proteins or antibodies. ..
  19. Mesoporus Diamondlike Carbon Medical Device Membrane
    Roger Narayan; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  20. Ultra-High-Throughput Screening (uHTS) Based on Surface*
    Tuan Vo Dinh; Fiscal Year: 2009
    ....
  21. ADVANCED MULTISPECTRAL IMAGING FOR MEDICAL DIAGNOSTICS
    Tuan Vo Dinh; Fiscal Year: 2005
    ..microelectroptics and molecular genetics and mouse studies. In addition, project personnel include a veterinarian at the University of Tennessee, and medical researchers with expertise in clinical studies at the TCSC. ..
  22. HYBRID HYDROGELS SELF-ASSEMBLED FROM GRAFT COPOLYMERS
    Jindrich Kopecek; Fiscal Year: 2008
    ..In addition, the possibility to join non-compatible materials, such as hydrophobic muscle mimics from liquid crystalline polymers and hydrogels, may open a new field of research. ..
  23. Lagrangian detection of biomolecular interactions
    FREDERICK HASELTON; Fiscal Year: 2005
    ..This technology will provide a new tool for the identification of patterns present in gene expression or protein profiles enabling these profiles to be fully utilized for diagnostic and prognostic purposes. ..
  24. Rapid Estimation of Gene Expression in Tissue Slices
    Alexander Asanov; Fiscal Year: 2003
    ..It will also permit physicians to better tailor subsequent treatments that may involve radiation and chemotherapy, treatments that can have debilitating side effects. ..
  25. TARGETING TO LYMPHOCYTES MEDIATED BY SYNTHETIC EPITOPES
    Jindrich Kopecek; Fiscal Year: 2004
    ..The results of the proposed studies will provide a new method to treat human lymphomas. ..
  26. DEGRADABLE HYDROGELS FOR ORAL DELIVERY OF CALCITONIN
    Jindrich Kopecek; Fiscal Year: 2001
    ..Based on in vivo animal data criteria will be studied. Based on in vivo animal data criteria will be established for the design of an oral hCT and CKI delivery system for the treatment of postmenopausal osteoporosis. ..
  27. DEGRADABLE HYDROGELS FOR ORAL DELIVERY OF CALCITONIN
    Jindrich Kopecek; Fiscal Year: 2003
    ..Based on in vivo animal data criteria will be studied. Based on in vivo animal data criteria will be established for the design of an oral hCT and CKI delivery system for the treatment of postmenopausal osteoporosis. ..
  28. Nuclease-Resistant Aptamers for Anthrax Opsonization
    John Bruno; Fiscal Year: 2004
    ..Such shielded aptamers could even be used in inhalers to enhance alveolar macrophage phagocytosis. ..
  29. Silicon Nano-Sensor: Single Molecule RNA/DNA Sequencing
    Rashid Bashir; Fiscal Year: 2002
    ....
  30. Retinal blood barrier permeability using optical tracers
    FREDERICK HASELTON; Fiscal Year: 2004
    ..abstract_text> ..
  31. Passivating Proteins in Implantable Glucose Sensors
    W Ward; Fiscal Year: 2004
    ..8. A histologic evaluation will be performed to assess the effect of passivating proteins and antibody blocked fibrinogen on the nature of the foreign body capsule. ..
  32. MRI Probes for Mapping Retinal Activity In Vivo
    Angelique Louie; Fiscal Year: 2004
    ..While our ultimate goal is to apply these agents in studies of macular degeneration, these agents would be useful for observations of any system in which membrane signaling activity plays a role. ..
  33. MODEL STUDIES OF LOW BARRIER HYDROGEN BONDS IN CATALYSIS
    Eric Anslyn; Fiscal Year: 2001
    ..The significance of such a result goes beyond an increased basic science understanding of enzymology, and has clear ramifications in the design of synthetic catalysts for biomedical and synthetic applications. ..
  34. Assesment of a Chronic Subcutaneous Glucose Sensor
    W Ward; Fiscal Year: 2003
    ..ether-based polymer. We will compare their long term function in studies using rabbits, which will be also be used to ascertain the effect of the growth factors and the real-time data processing. ..
  35. Rapid Detection of Major Food-borne Pathogens
    Peixuan Zhu; Fiscal Year: 2003
    ..The instrument will be redesigned to be more compact and portable, and assays developed for use outside a laboratory setting. Tests will be performed on location where these foods are produced, transported and/or prepared. ..
  36. Home testing of 24/7 closed-loop control in children and adolescents with type 1
    Roman Hovorka; Fiscal Year: 2010
    ..This will be preceded by evaluating 24/7 closed-loop control in compliant and non-compliant subjects in clinical research centre settings to collect data supporting regulatory submission for home testing. ..
  37. FLUORESCENCE SYSTEM FOR SENSING BIOSPECIFIC INTERACTIONS
    Alexander Asanov; Fiscal Year: 2003
    ....
  38. MOLECULAR ANALYSIS OF ANCHORING PROTEINS
    John Scott; Fiscal Year: 2003
    ....
  39. Assay and Instrument Development for Diagnosis of Prion Disease
    Peixuan Zhu; Fiscal Year: 2007
    ..Current tests are all postmortem assays using brain tissue. This proposal is to develop a sensitive blood test to detect the disease in its early stage. [unreadable] [unreadable] [unreadable]..
  40. Nanoarray-Type Detection of Oct3/4 and Cdx2 using AuNPs and E-beam Patterns
    Jeong Yeol Yoon; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  41. REGULATION OF CYTOSKELETON AND MEMBRANE TRAFFIC BY P22
    Margarida Barroso; Fiscal Year: 2002
    ....
  42. Nuclease-Resistant Aptamers to Botox and Anthrax Toxins
    John Bruno; Fiscal Year: 2004
    ..Such reagents could also be used in environmental biosensors or diagnostic test kits for detection of BOTOX and anthrax toxins in body fluids or environmental samples. ..
  43. INFRASTRUCTURE OF BIOENGINEERING DEVELOPMENT
    David Gough; Fiscal Year: 2002
    ..This is necessary to develop biomedical engineering at UCSD to its full potential and to provide a model of bioengineering education and research for the nation. ..
  44. MECHANISM OF KINESIN ATPASE
    David Hackney; Fiscal Year: 2002
    ..These enzymatic studies will be coupled with study of the motility that kinesin is able to produce. A long range goal is to be able to model the physical properties of kinesin based on its solution biochemical mechanism, ..
  45. Validation of Long-Term Glucose Sensor in Tissues
    David Gough; Fiscal Year: 2007
    ..This project will further validate an implantable glucose sensor that will provide a basis for reliable detection of hypoglycemia and automatic insulin administration. [unreadable] [unreadable] [unreadable]..
  46. Pathogen-sensing with novel poly(aryleneethynylene)s
    UWE BUNZ; Fiscal Year: 2003
    ..The sensing event should be detectable by simple inspection, or in more sophisticated cases a simple fluorescence spectrometer will be utilized. ..
  47. Continuous Sequencing-By-Synthesis Based on a Digital Microfluidic Platform
    RICHARD FAIR; Fiscal Year: 2009
    ....
  48. USE OF A TACTILE BREAST IMAGER FOR MASS PRE-SCREENING
    Armen Sarvazyan; Fiscal Year: 2002
    ..The potential gross revenues on the sale of this device is $520,000,000. ..
  49. Droplet-Based Digital Microfluidic Genome Sequencing
    RICHARD FAIR; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  50. Implantable MEMS Drug Delivery Device for Glaucoma Management
    ELLIS MENG; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  51. Diagnostic Technology Based on Acoustic Radiation Force
    Armen Sarvazyan; Fiscal Year: 2004
    ....
  52. An In-Situ Biliary Manometer for Sphincter of Oddi Dysfunction Diagnosis
    KEAT ONG; Fiscal Year: 2008
    ..As an example, with proper modifications the same sensor technology can be applied for measuring the pressure within an artery stent for in-stent stenosis detection. [unreadable] [unreadable] [unreadable]..
  53. Catalytic DNA Biosensor for Toxic Metal Ions
    Yi Lu; Fiscal Year: 2005
    ..abstract_text> ..
  54. IMAGING NETWORK FOR BREAST CANCER MASS SCREENING
    Armen Sarvazyan; Fiscal Year: 2007
    ..Appropriate regulatory testing will be conducted in order to prepare necessary engineering documentation for a FDA application. ..
  55. A Unified Reporter Gene for Multi-Modality Imaging
    Jianghong Rao; Fiscal Year: 2005
    ..abstract_text> ..
  56. MAGNETOELASTIC SENSORS FOR BIOMEDICAL MONITORING
    KEAT ONG; Fiscal Year: 2003
    ..The wireless, remote query nature of the sensor technology would also find great utility for in-situ monitoring of biological chemical analyte concentrations such as glucose. ..